Chy12G214320 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G214320
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like
Location	chrH12: 13533243 .. 13535027 (-)
RNA-Seq Expression	Chy12G214320
Synteny	Chy12G214320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGCACAACTACCCCCCAGATCCGACGCCGGCCACCCCTGCCGTCCACTCCAATTCCCTTTGCAGGTCATCACCGGTCGCTGGTTCACCGTCTTTGCTACATTTTTAATCATGGCTGGCGCCGGTGCTACCTATCTCTTCGGTGTCTACTCCAAACAGATCAAATCCGCACTTGGGTATGATCAAACCACTTTGAATCTGATGGGCTTTTTCAAAGATCTGGGTGCTAACGTCGGCGTGTTGTCTGGTTTAGTGGCGGAGGTTACGCCCACTTGGTTCGTTCTTCTCCTTGGCTCCGCCTTGAACTTCACCGGTTACTTCATGATTTGGCTTGCCGTCAGCGGCAGAATCGCCAAGCCCAAAGTCTGGCAAATGTGTCTCTACATTTGTGTTGGAGCTAATTCCCAGAATTTTGCTAATACCGGCGCTTTGGTTACTTGTATCCAGAACTTTCCCGAGAGTCGCGGCGCTATGATGGGTCTTTTGAAGGGTTTCACAGGGCTTAGCGGCGCTATTCTCACGGAGATTTATAGGGCCGTTTATGCCGACGATGCCACGGCGTTGATTCTACTTATTGGGTGGCTTCCGGCGGCGATTTCCGTCGTTTTTGTTTTCACCATTCGGAGGCTTAGATCAGAGAGACAACCGAATGAGAAGAGGGTTTTTTACCATTTCCTTTACATCTCAATTGGGTTAGCGGTTTTCATCATGATTATGAATATTGTTCAGAAGAAAGTTCAATTCAATCATACAGCTTATGCTTCAAGTGCTACCGTGATCTGCGCTTTTCTGTTTCTTCCTCTGTTAATTGTGATCCGTGAAGAACTTCGTATCTGGAATACAAAGAAATCTACCTCAGTTCCAATCGAAAGCCCACAACCAAAACCAATCGATGAACCCAAAATCATAACAGAAGAATCAAAACAAATCACTGAGATCCAAAACCAAAACGCAGCAGCACCACCACCAGAGTCCTGTTTCTCCAACATTTGTCATAAACCACCCAGAGGAGACGATTACACAATCCTTCAAGCACTTCTCAGCATCGATATGTTCGTTCTATTCGTCGCAACATTCTGTGGGTTAGGTACAAGCTTAACCGCAGTTGACAATTTAGGACAAATCGGTGAATCACTCGGCTACCCATTAAAAACAGTAAGCTCATTCGTTTCGTTAGTAAGCATTTGGAATTACTTCGGCAGAATATTCGCCGGATTCGTTTCCGAATCCCTTCTCGCAAAATTCAAATTCCCAAGACCATTAATGATGACATTAGTTCTTCTCCTCTCTTGCGTCGGCCAACTCTTAATCGCATTCCCAGTTCCGGGTTCCGTCTACATAGCTTCAGTAATAATCGGATTCTCCTTCGGCGCACAGCTTCCTCTGCTTTTCGCCATAATCTCTGAACTATTTGGACTGAAATATTTCTCCACTTTATTCAATTGTGGGCAAATAGCAAGCCCATTGGGATCGTACATTCTGAATGTGAAGGTGGCGGGAATGTTGTACGATATGGAAGCTTTGAAGCAGTTGAAGGAGAAGGGGTTGGATAGATCGGCGGTGAAGGAATTGATATGCATGGGGAAGCAATGTTACAGGAAATCATTTTCAATAACGGCGATTGTTACGTTTGTTGGAGCAATGGTTTCGTTGATATTGGTGATGAGAACGAGGGAGTTTTACAAAGGGGATATTTACAAAAAGTTTAGGGAAGAAGGAATGAAGAAAGAGGAGATGGTCATCAACGGCGGAGGAGAAGTTACTGCTCGGGAATCTAAGTGA

mRNA sequence

ATGGTTGCACAACTACCCCCCAGATCCGACGCCGGCCACCCCTGCCGTCCACTCCAATTCCCTTTGCAGGTCATCACCGGTCGCTGGTTCACCGTCTTTGCTACATTTTTAATCATGGCTGGCGCCGGTGCTACCTATCTCTTCGGTGTCTACTCCAAACAGATCAAATCCGCACTTGGGTATGATCAAACCACTTTGAATCTGATGGGCTTTTTCAAAGATCTGGGTGCTAACGTCGGCGTGTTGTCTGGTTTAGTGGCGGAGGTTACGCCCACTTGGTTCGTTCTTCTCCTTGGCTCCGCCTTGAACTTCACCGGTTACTTCATGATTTGGCTTGCCGTCAGCGGCAGAATCGCCAAGCCCAAAGTCTGGCAAATGTGTCTCTACATTTGTGTTGGAGCTAATTCCCAGAATTTTGCTAATACCGGCGCTTTGGTTACTTGTATCCAGAACTTTCCCGAGAGTCGCGGCGCTATGATGGGTCTTTTGAAGGGTTTCACAGGGCTTAGCGGCGCTATTCTCACGGAGATTTATAGGGCCGTTTATGCCGACGATGCCACGGCGTTGATTCTACTTATTGGGTGGCTTCCGGCGGCGATTTCCGTCGTTTTTGTTTTCACCATTCGGAGGCTTAGATCAGAGAGACAACCGAATGAGAAGAGGGTTTTTTACCATTTCCTTTACATCTCAATTGGGTTAGCGGTTTTCATCATGATTATGAATATTGTTCAGAAGAAAGTTCAATTCAATCATACAGCTTATGCTTCAAGTGCTACCGTGATCTGCGCTTTTCTGTTTCTTCCTCTGTTAATTGTGATCCGTGAAGAACTTCGTATCTGGAATACAAAGAAATCTACCTCAGTTCCAATCGAAAGCCCACAACCAAAACCAATCGATGAACCCAAAATCATAACAGAAGAATCAAAACAAATCACTGAGATCCAAAACCAAAACGCAGCAGCACCACCACCAGAGTCCTGTTTCTCCAACATTTGTCATAAACCACCCAGAGGAGACGATTACACAATCCTTCAAGCACTTCTCAGCATCGATATGTTCGTTCTATTCGTCGCAACATTCTGTGGGTTAGGTACAAGCTTAACCGCAGTTGACAATTTAGGACAAATCGGTGAATCACTCGGCTACCCATTAAAAACAGTAAGCTCATTCGTTTCGTTAGTAAGCATTTGGAATTACTTCGGCAGAATATTCGCCGGATTCGTTTCCGAATCCCTTCTCGCAAAATTCAAATTCCCAAGACCATTAATGATGACATTAGTTCTTCTCCTCTCTTGCGTCGGCCAACTCTTAATCGCATTCCCAGTTCCGGGTTCCGTCTACATAGCTTCAGTAATAATCGGATTCTCCTTCGGCGCACAGCTTCCTCTGCTTTTCGCCATAATCTCTGAACTATTTGGACTGAAATATTTCTCCACTTTATTCAATTGTGGGCAAATAGCAAGCCCATTGGGATCGTACATTCTGAATGTGAAGGTGGCGGGAATGTTGTACGATATGGAAGCTTTGAAGCAGTTGAAGGAGAAGGGGTTGGATAGATCGGCGGTGAAGGAATTGATATGCATGGGGAAGCAATGTTACAGGAAATCATTTTCAATAACGGCGATTGTTACGTTTGTTGGAGCAATGGTTTCGTTGATATTGGTGATGAGAACGAGGGAGTTTTACAAAGGGGATATTTACAAAAAGTTTAGGGAAGAAGGAATGAAGAAAGAGGAGATGGTCATCAACGGCGGAGGAGAAGTTACTGCTCGGGAATCTAAGTGA

Coding sequence (CDS)

ATGGTTGCACAACTACCCCCCAGATCCGACGCCGGCCACCCCTGCCGTCCACTCCAATTCCCTTTGCAGGTCATCACCGGTCGCTGGTTCACCGTCTTTGCTACATTTTTAATCATGGCTGGCGCCGGTGCTACCTATCTCTTCGGTGTCTACTCCAAACAGATCAAATCCGCACTTGGGTATGATCAAACCACTTTGAATCTGATGGGCTTTTTCAAAGATCTGGGTGCTAACGTCGGCGTGTTGTCTGGTTTAGTGGCGGAGGTTACGCCCACTTGGTTCGTTCTTCTCCTTGGCTCCGCCTTGAACTTCACCGGTTACTTCATGATTTGGCTTGCCGTCAGCGGCAGAATCGCCAAGCCCAAAGTCTGGCAAATGTGTCTCTACATTTGTGTTGGAGCTAATTCCCAGAATTTTGCTAATACCGGCGCTTTGGTTACTTGTATCCAGAACTTTCCCGAGAGTCGCGGCGCTATGATGGGTCTTTTGAAGGGTTTCACAGGGCTTAGCGGCGCTATTCTCACGGAGATTTATAGGGCCGTTTATGCCGACGATGCCACGGCGTTGATTCTACTTATTGGGTGGCTTCCGGCGGCGATTTCCGTCGTTTTTGTTTTCACCATTCGGAGGCTTAGATCAGAGAGACAACCGAATGAGAAGAGGGTTTTTTACCATTTCCTTTACATCTCAATTGGGTTAGCGGTTTTCATCATGATTATGAATATTGTTCAGAAGAAAGTTCAATTCAATCATACAGCTTATGCTTCAAGTGCTACCGTGATCTGCGCTTTTCTGTTTCTTCCTCTGTTAATTGTGATCCGTGAAGAACTTCGTATCTGGAATACAAAGAAATCTACCTCAGTTCCAATCGAAAGCCCACAACCAAAACCAATCGATGAACCCAAAATCATAACAGAAGAATCAAAACAAATCACTGAGATCCAAAACCAAAACGCAGCAGCACCACCACCAGAGTCCTGTTTCTCCAACATTTGTCATAAACCACCCAGAGGAGACGATTACACAATCCTTCAAGCACTTCTCAGCATCGATATGTTCGTTCTATTCGTCGCAACATTCTGTGGGTTAGGTACAAGCTTAACCGCAGTTGACAATTTAGGACAAATCGGTGAATCACTCGGCTACCCATTAAAAACAGTAAGCTCATTCGTTTCGTTAGTAAGCATTTGGAATTACTTCGGCAGAATATTCGCCGGATTCGTTTCCGAATCCCTTCTCGCAAAATTCAAATTCCCAAGACCATTAATGATGACATTAGTTCTTCTCCTCTCTTGCGTCGGCCAACTCTTAATCGCATTCCCAGTTCCGGGTTCCGTCTACATAGCTTCAGTAATAATCGGATTCTCCTTCGGCGCACAGCTTCCTCTGCTTTTCGCCATAATCTCTGAACTATTTGGACTGAAATATTTCTCCACTTTATTCAATTGTGGGCAAATAGCAAGCCCATTGGGATCGTACATTCTGAATGTGAAGGTGGCGGGAATGTTGTACGATATGGAAGCTTTGAAGCAGTTGAAGGAGAAGGGGTTGGATAGATCGGCGGTGAAGGAATTGATATGCATGGGGAAGCAATGTTACAGGAAATCATTTTCAATAACGGCGATTGTTACGTTTGTTGGAGCAATGGTTTCGTTGATATTGGTGATGAGAACGAGGGAGTTTTACAAAGGGGATATTTACAAAAAGTTTAGGGAAGAAGGAATGAAGAAAGAGGAGATGGTCATCAACGGCGGAGGAGAAGTTACTGCTCGGGAATCTAAGTGA

Protein sequence

MVAQLPPRSDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEESKQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPRPLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVSLILVMRTREFYKGDIYKKFREEGMKKEEMVINGGGEVTARESK*

Homology

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BLAST of Chy12G214320 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 6.4e-31
Identity = 142/553 (25.68%), Postives = 243/553 (43.94%), Query Frame = 0


Query: 21  PLQVITGRW--FTVF-ATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 80
           PL+ + GRW  +TV  A   I A  G  + F  YS  +KS LG  Q  LN +    DLG 
Sbjct: 33  PLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGK 92

Query: 81  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGAN-S 140
             G  SG+     P   VL   +A+ F GY + WL ++  I  P  + +    C+ A  S
Sbjct: 93  AFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLS 152

Query: 141 QNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWL 200
             + NT   + CI++FP +R   + L   F G+S A+ +  + A+    +   +LL   +
Sbjct: 153 ICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLV 212

Query: 201 PAAISVVF---VFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTA 260
           P  +S      V T   L +    + +R   H   I   LAV      ++      +   
Sbjct: 213 PLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARL 272

Query: 261 YASSATVICAF-LFLPLLIVIREE-LRIWNTKKSTSVPIESPQPKPIDEPKIITEESKQI 320
               A V+  F L  PLL+  R+  L + N + +     ES     ++    I E   Q 
Sbjct: 273 NFIGAVVLLVFPLCAPLLVYARDYFLPVINARLNH----ESSGYVMLN----IDELKNQK 332

Query: 321 TEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVD 380
           T + ++        +   N       GD+++    +  ++ ++ ++A FCG    L   +
Sbjct: 333 TSVSSKTGYEHMGTAKEGNTVR---LGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSN 392

Query: 381 NLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPRPLMMTLVLLLS 440
           NLGQI +SLG   +  ++ V++ S +++FGR+ +    + +  +F+  R     + LL +
Sbjct: 393 NLGQIAQSLG---QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPT 452

Query: 441 CVGQLLIAF--PVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASP 500
            +   L+A       ++  A+ +IG S G       +I S+LFG        N      P
Sbjct: 453 PIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIP 512

Query: 501 LGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAM 560
           +GS +L   +A  +Y+  A   +     D      ++C+G+ CY K+F     ++ +G +
Sbjct: 513 IGS-LLYGYIAASIYEANASPDITPIVSD-----SIVCIGRDCYFKTFVFWGCLSILGVV 562

Query: 561 VSLILVMRTREFY 563
            SL L +RT+  Y
Sbjct: 573 SSLSLYIRTKPVY 562

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BLAST of Chy12G214320 vs. ExPASy TrEMBL
Match: A0A0A0LXR5 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G097655 PE=4 SV=1)

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 584/594 (98.32%), Postives = 587/594 (98.82%), Query Frame = 0


Query: 1   MVAQLPPRSDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60
           MVAQLPPRSDAGH CRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG
Sbjct: 1   MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60

Query: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120
           YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK
Sbjct: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120

Query: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180
           PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA
Sbjct: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180

Query: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240
           VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM
Sbjct: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240

Query: 241 NIVQKKVQFNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDE 300
           NIVQKKVQFNHTAYASSATVIC FLFLPLL+VIREELRIWNTKKSTSVPIESPQPKPIDE
Sbjct: 241 NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE 300

Query: 301 PKIITEESKQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATF 360
           PKIITEESKQITEIQ QN A PPPESCFSNIC KPPRGDDYTILQALLSIDMFVLFVATF
Sbjct: 301 PKIITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATF 360

Query: 361 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPR 420
           CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLA+FKFPR
Sbjct: 361 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPR 420

Query: 421 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 480
           PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL
Sbjct: 421 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 480

Query: 481 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 540
           FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT
Sbjct: 481 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 540

Query: 541 AIVTFVGAMVSLILVMRTREFYKGDIYKKFREEGMKKEEMVINGGGEVTARESK 595
           AIVTFVGAMVSL+LVMRTREFYKGDIYKKFREE MKKEEMVINGGGEVTARESK
Sbjct: 541 AIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 594

[Close]

BLAST of Chy12G214320 vs. ExPASy TrEMBL
Match: A0A5A7UED5 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold609G00250 PE=4 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.2e-310
Identity = 547/592 (92.40%), Postives = 570/592 (96.28%), Query Frame = 0


Query: 1   MVAQLPPRSDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60
           MV+Q P  SDA HP RPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG
Sbjct: 1   MVSQSPTSSDASHPRRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60

Query: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120
           YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAK
Sbjct: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAK 120

Query: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180
           PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA
Sbjct: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180

Query: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240
           VYADDATALILLIGWLPAAISVVFVFTIRRL++ERQPNEKRVFYHFLYISIGLAVFIMIM
Sbjct: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLKAERQPNEKRVFYHFLYISIGLAVFIMIM 240

Query: 241 NIVQKKVQFNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDE 300
           NIVQKKV+FNHTAY  SATVIC FLFLPLLIVIREELR+WN  KS  +PIE+PQPKPIDE
Sbjct: 241 NIVQKKVEFNHTAYTLSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENPQPKPIDE 300

Query: 301 PKIITEESKQITEIQNQNAAAPPPE-SCFSNICHKPPRGDDYTILQALLSIDMFVLFVAT 360
           PKIITEES QIT+IQNQ+  APPPE +CFSNIC KPPRGDDYTILQALLSIDMFVLF+AT
Sbjct: 301 PKIITEESNQITKIQNQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIAT 360

Query: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFP 420
           FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLA++KFP
Sbjct: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFP 420

Query: 421 RPLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480
           RPLMMTLVLLLSC+GQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST
Sbjct: 421 RPLMMTLVLLLSCIGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480

Query: 481 LFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSI 540
           LFNCGQIASPLGSYILNVKVAGMLYD+EALKQLKEKGLDRSAVKEL C+GK+CYRKSF+I
Sbjct: 481 LFNCGQIASPLGSYILNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTI 540

Query: 541 TAIVTFVGAMVSLILVMRTREFYKGDIYKKFREEGMKKEEMVINGGGEVTAR 592
           T IVTF GAMVSLILVMRTREFYKGDIYKKFREE +KKEE +INGGGE+TA+
Sbjct: 541 TTIVTFAGAMVSLILVMRTREFYKGDIYKKFREEEVKKEEKIINGGGEITAK 592

[Close]

BLAST of Chy12G214320 vs. ExPASy TrEMBL
Match: A0A1S3CP80 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103503227 PE=4 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.2e-310
Identity = 547/592 (92.40%), Postives = 570/592 (96.28%), Query Frame = 0


Query: 1   MVAQLPPRSDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60
           MV+Q P  SDA HP RPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG
Sbjct: 1   MVSQSPTSSDASHPRRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60

Query: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120
           YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAK
Sbjct: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAK 120

Query: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180
           PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA
Sbjct: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180

Query: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240
           VYADDATALILLIGWLPAAISVVFVFTIRRL++ERQPNEKRVFYHFLYISIGLAVFIMIM
Sbjct: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLKAERQPNEKRVFYHFLYISIGLAVFIMIM 240

Query: 241 NIVQKKVQFNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDE 300
           NIVQKKV+FNHTAY  SATVIC FLFLPLLIVIREELR+WN  KS  +PIE+PQPKPIDE
Sbjct: 241 NIVQKKVEFNHTAYTLSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENPQPKPIDE 300

Query: 301 PKIITEESKQITEIQNQNAAAPPPE-SCFSNICHKPPRGDDYTILQALLSIDMFVLFVAT 360
           PKIITEES QIT+IQNQ+  APPPE +CFSNIC KPPRGDDYTILQALLSIDMFVLF+AT
Sbjct: 301 PKIITEESNQITKIQNQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIAT 360

Query: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFP 420
           FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLA++KFP
Sbjct: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFP 420

Query: 421 RPLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480
           RPLMMTLVLLLSC+GQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST
Sbjct: 421 RPLMMTLVLLLSCIGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480

Query: 481 LFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSI 540
           LFNCGQIASPLGSYILNVKVAGMLYD+EALKQLKEKGLDRSAVKEL C+GK+CYRKSF+I
Sbjct: 481 LFNCGQIASPLGSYILNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTI 540

Query: 541 TAIVTFVGAMVSLILVMRTREFYKGDIYKKFREEGMKKEEMVINGGGEVTAR 592
           T IVTF GAMVSLILVMRTREFYKGDIYKKFREE +KKEE +INGGGE+TA+
Sbjct: 541 TTIVTFAGAMVSLILVMRTREFYKGDIYKKFREEEVKKEEKIINGGGEITAK 592

[Close]

BLAST of Chy12G214320 vs. ExPASy TrEMBL
Match: A0A6J1HPV2 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464958 PE=4 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 2.4e-270
Identity = 485/577 (84.06%), Postives = 524/577 (90.81%), Query Frame = 0


Query: 18  LQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 77
           LQF L V+ GRWFTVFATFLIMAGAGATYLFGVYSKQIKS LGYDQTTLNLMGFFKDLGA
Sbjct: 20  LQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNLMGFFKDLGA 79

Query: 78  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQ 137
           NVGVLSGLVAEVTPTWFVLLLGS LNFTGYFMIWLAV+GRI KPKVWQMCLYICVGANSQ
Sbjct: 80  NVGVLSGLVAEVTPTWFVLLLGSVLNFTGYFMIWLAVTGRITKPKVWQMCLYICVGANSQ 139

Query: 138 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLP 197
           NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIY AVY +DATALILLI WLP
Sbjct: 140 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATALILLIAWLP 199

Query: 198 AAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASS 257
           AA+SVVF+F IR+L++ERQPNE RVFYHFLYISIGLA+FIM+MNI+QKKV+FNHTAYA S
Sbjct: 200 AALSVVFIFNIRKLKAERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVEFNHTAYALS 259

Query: 258 ATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEESKQITEIQNQ 317
           ATVICA LFLPL IVIREEL IWN+K +  +PIE+P      EPKI  EESK+I    + 
Sbjct: 260 ATVICALLFLPLFIVIREELHIWNSKINPPIPIENP-----IEPKISIEESKEIKNNPDP 319

Query: 318 NAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVDNLGQIG 377
               PPP SCFS+IC+KP RGDDYTILQALLSIDM VLF+ATFCGLGTSLTAVDNLGQIG
Sbjct: 320 K-PYPPPPSCFSSICNKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLTAVDNLGQIG 379

Query: 378 ESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPRPLMMTLVLLLSCVGQLL 437
           ESLGYPLKTV SFVSLVSIWNYFGR+F+GFVSE+LLAKFKFPRPLMMTLVLLLSC+GQLL
Sbjct: 380 ESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSCIGQLL 439

Query: 438 IAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNV 497
           IAFP+PGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNV
Sbjct: 440 IAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNV 499

Query: 498 KVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVSLILVMR 557
           KVAGM+YD EALKQLKE G+DR+AVKEL C+GKQCYRKSF++ AIVTFVGA+VSL+LVMR
Sbjct: 500 KVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKQCYRKSFTLMAIVTFVGALVSLVLVMR 559

Query: 558 TREFYKGDIYKKFREEGMKKEEMVINGGGEVTARESK 595
           TREFY+GDIYK+FREE +K+E  V    GE  A ESK
Sbjct: 560 TREFYRGDIYKRFREE-VKQETSV----GEAAAVESK 585

[Close]

BLAST of Chy12G214320 vs. ExPASy TrEMBL
Match: A0A6J1I026 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469244 PE=4 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 4.0e-270
Identity = 485/586 (82.76%), Postives = 528/586 (90.10%), Query Frame = 0


Query: 9   SDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNL 68
           +DAG     LQF L V+ GRWFTVFATFLIMAGAGATYLFGVYSKQIKS LGYDQTTLNL
Sbjct: 11  ADAGRGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNL 70

Query: 69  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCL 128
           MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGS LNFTGYFMIWLAV+GRIAKPKVWQMCL
Sbjct: 71  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSVLNFTGYFMIWLAVTGRIAKPKVWQMCL 130

Query: 129 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATA 188
           YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILT+IY AVY +DATA
Sbjct: 131 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGEDATA 190

Query: 189 LILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQ 248
           LILLI WLPAA+SVVF+F IR+L++ER+PNE RVFYHFLY+SIGLA+FIM+MNI+QK+V+
Sbjct: 191 LILLIAWLPAALSVVFIFNIRKLKAERRPNETRVFYHFLYVSIGLAIFIMLMNIIQKEVE 250

Query: 249 FNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEES 308
           FNHTAYA SATVICA LFLPL IVIREEL+IWN+K +  +PIE+P      EPKI T+ES
Sbjct: 251 FNHTAYALSATVICALLFLPLFIVIREELQIWNSKINPPIPIENP-----TEPKISTDES 310

Query: 309 KQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLT 368
           K+I    +     PPP SCFS+IC KP RGDDYTILQALLSIDM VLF+ATFCGLGTSLT
Sbjct: 311 KEIKNNPDPK-PYPPPPSCFSSICDKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLT 370

Query: 369 AVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPRPLMMTLVL 428
           AVDNLGQIGESLGYPLKTV SFVSLVSIWNYFGR+F+GFVSE+LLAKFKFPRPLMMTLVL
Sbjct: 371 AVDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVL 430

Query: 429 LLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 488
           LLSC+GQLLIAFP+PGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS
Sbjct: 431 LLSCIGQLLIAFPIPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 490

Query: 489 PLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGA 548
           PLGSYILNVKVAGM+YD EALKQLKE G+DR+AVKEL C+GK+CYRKSF++ AIVTFVGA
Sbjct: 491 PLGSYILNVKVAGMMYDAEALKQLKEMGIDRAAVKELTCIGKRCYRKSFTLMAIVTFVGA 550

Query: 549 MVSLILVMRTREFYKGDIYKKFREEGMKKEEMVINGGGEVTARESK 595
           +VSL+LVMRTREFY+GDIYK+FREE   KEE      GE  A ESK
Sbjct: 551 LVSLVLVMRTREFYRGDIYKRFREE--VKEE---TSRGEAAAVESK 585

[Close]

BLAST of Chy12G214320 vs. NCBI nr
Match: XP_004147280.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN64786.1 hypothetical protein Csa_014287 [Cucumis sativus])

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 584/594 (98.32%), Postives = 587/594 (98.82%), Query Frame = 0


Query: 1   MVAQLPPRSDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60
           MVAQLPPRSDAGH CRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG
Sbjct: 1   MVAQLPPRSDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60

Query: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120
           YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK
Sbjct: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120

Query: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180
           PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA
Sbjct: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180

Query: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240
           VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM
Sbjct: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240

Query: 241 NIVQKKVQFNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDE 300
           NIVQKKVQFNHTAYASSATVIC FLFLPLL+VIREELRIWNTKKSTSVPIESPQPKPIDE
Sbjct: 241 NIVQKKVQFNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDE 300

Query: 301 PKIITEESKQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATF 360
           PKIITEESKQITEIQ QN A PPPESCFSNIC KPPRGDDYTILQALLSIDMFVLFVATF
Sbjct: 301 PKIITEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATF 360

Query: 361 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPR 420
           CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLA+FKFPR
Sbjct: 361 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPR 420

Query: 421 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 480
           PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL
Sbjct: 421 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 480

Query: 481 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 540
           FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT
Sbjct: 481 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 540

Query: 541 AIVTFVGAMVSLILVMRTREFYKGDIYKKFREEGMKKEEMVINGGGEVTARESK 594
           AIVTFVGAMVSL+LVMRTREFYKGDIYKKFREE MKKEEMVINGGGEVTARESK
Sbjct: 541 AIVTFVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 594

[Close]

BLAST of Chy12G214320 vs. NCBI nr
Match: XP_008465591.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0051859.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK21380.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 547/592 (92.40%), Postives = 570/592 (96.28%), Query Frame = 0


Query: 1   MVAQLPPRSDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60
           MV+Q P  SDA HP RPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG
Sbjct: 1   MVSQSPTSSDASHPRRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALG 60

Query: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAK 120
           YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAK
Sbjct: 61  YDQTTLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAK 120

Query: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180
           PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA
Sbjct: 121 PKVWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRA 180

Query: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIM 240
           VYADDATALILLIGWLPAAISVVFVFTIRRL++ERQPNEKRVFYHFLYISIGLAVFIMIM
Sbjct: 181 VYADDATALILLIGWLPAAISVVFVFTIRRLKAERQPNEKRVFYHFLYISIGLAVFIMIM 240

Query: 241 NIVQKKVQFNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDE 300
           NIVQKKV+FNHTAY  SATVIC FLFLPLLIVIREELR+WN  KS  +PIE+PQPKPIDE
Sbjct: 241 NIVQKKVEFNHTAYTLSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENPQPKPIDE 300

Query: 301 PKIITEESKQITEIQNQNAAAPPPE-SCFSNICHKPPRGDDYTILQALLSIDMFVLFVAT 360
           PKIITEES QIT+IQNQ+  APPPE +CFSNIC KPPRGDDYTILQALLSIDMFVLF+AT
Sbjct: 301 PKIITEESNQITKIQNQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIAT 360

Query: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFP 420
           FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLA++KFP
Sbjct: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFP 420

Query: 421 RPLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480
           RPLMMTLVLLLSC+GQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST
Sbjct: 421 RPLMMTLVLLLSCIGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFST 480

Query: 481 LFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSI 540
           LFNCGQIASPLGSYILNVKVAGMLYD+EALKQLKEKGLDRSAVKEL C+GK+CYRKSF+I
Sbjct: 481 LFNCGQIASPLGSYILNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTI 540

Query: 541 TAIVTFVGAMVSLILVMRTREFYKGDIYKKFREEGMKKEEMVINGGGEVTAR 591
           T IVTF GAMVSLILVMRTREFYKGDIYKKFREE +KKEE +INGGGE+TA+
Sbjct: 541 TTIVTFAGAMVSLILVMRTREFYKGDIYKKFREEEVKKEEKIINGGGEITAK 592

[Close]

BLAST of Chy12G214320 vs. NCBI nr
Match: XP_038895725.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 981 bits (2536), Expect = 0.0
Identity = 501/573 (87.43%), Postives = 532/573 (92.84%), Query Frame = 0


Query: 9   SDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNL 68
           +D  H C  L+F LQVI GRWFTVFATFLIMAGAGATYLFGVYSKQIK+ LGYDQTTLNL
Sbjct: 7   ADGAHGCAALRFTLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKATLGYDQTTLNL 66

Query: 69  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCL 128
           MGF KDLGANVGVLSGLVAEVTPTWFVLLLGSALNF GYFMIWLAV+GRIAKP+VWQMCL
Sbjct: 67  MGFSKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFAGYFMIWLAVTGRIAKPQVWQMCL 126

Query: 129 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATA 188
           YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGA+LT+IY AVY DDATA
Sbjct: 127 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAVLTQIYLAVYGDDATA 186

Query: 189 LILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQ 248
           LILLIGWLPAA+SVVFVFTIRRLR+ERQPNEKRVFYHFLY+SIGLA+FIMIMNIVQKKV+
Sbjct: 187 LILLIGWLPAALSVVFVFTIRRLRAERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKKVE 246

Query: 249 FNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPI--ESPQ------PKPIDE 308
           FNHTAYA SATVIC FLFLPL IVIREEL +WN KK+ + PI  E+PQ      PKP+DE
Sbjct: 247 FNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKTPTPPIPNENPQSQSQSQPKPVDE 306

Query: 309 PKIITEESKQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATF 368
            KIITEES QI EI N     PPP+SCFSNICHKP RGDDYTILQALLS+DM VLF+ATF
Sbjct: 307 SKIITEESNQIKEIPN-----PPPDSCFSNICHKPARGDDYTILQALLSVDMLVLFIATF 366

Query: 369 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPR 428
           CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGR+F+GFVSE+LLAKFKFPR
Sbjct: 367 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPR 426

Query: 429 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 488
           PLMMTLVLLLSC+GQLLIAFPVPGSVY+ASVIIGFSFGAQLPLLFAIISELFGLKYFSTL
Sbjct: 427 PLMMTLVLLLSCIGQLLIAFPVPGSVYLASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 486

Query: 489 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 548
           FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKEL C+GKQC+RKSF++ 
Sbjct: 487 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELTCIGKQCFRKSFTLM 546

Query: 549 AIVTFVGAMVSLILVMRTREFYKGDIYKKFREE 573
           AIVTFVGAMVSL+LVMRTREFY+GDIYKKFR E
Sbjct: 547 AIVTFVGAMVSLVLVMRTREFYRGDIYKKFRGE 574

[Close]

BLAST of Chy12G214320 vs. NCBI nr
Match: XP_023519125.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 951 bits (2457), Expect = 0.0
Identity = 489/586 (83.45%), Postives = 529/586 (90.27%), Query Frame = 0


Query: 9   SDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNL 68
           +DAG     LQF L V+ GRWFTVFATFLIMAGAGATYLFGVYSKQIKS LGYDQTTLNL
Sbjct: 11  ADAGRGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNL 70

Query: 69  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCL 128
           MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAKPKVWQMCL
Sbjct: 71  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQMCL 130

Query: 129 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATA 188
           YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIY AVY +DATA
Sbjct: 131 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATA 190

Query: 189 LILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQ 248
           LILLI WLPAA+SVVF+F IR+L++ERQPNE RVFYHFLYISIGLA+FIM+MNI+QKKV+
Sbjct: 191 LILLIAWLPAALSVVFIFNIRKLKAERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVE 250

Query: 249 FNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEES 308
           FNHTAYA SATVICA LFLPL IVIREEL IWN+K +  +PIE+P    I EPKI  EES
Sbjct: 251 FNHTAYALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENPIEPKIXEPKISIEES 310

Query: 309 KQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLT 368
           K+I    +     PPP SCFS+IC KP RGDDYTILQALLSIDM VLF+ATFCGLGTSLT
Sbjct: 311 KEIKNNPDPKPYPPPP-SCFSSICSKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLT 370

Query: 369 AVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPRPLMMTLVL 428
           AVDNLGQIGESLGYPLKTV SFVSLVSIWNYFGR+F+GFVSE+LLAKFKFPRPLMMTLVL
Sbjct: 371 AVDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVL 430

Query: 429 LLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 488
           LLSC+GQLLIAFP+PGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS
Sbjct: 431 LLSCIGQLLIAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 490

Query: 489 PLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGA 548
           PLGSYILNVKVAGM+YD EALKQLKE G+DR+AVKEL C+GK+CYRKSF++ AIVTFVGA
Sbjct: 491 PLGSYILNVKVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKRCYRKSFTLMAIVTFVGA 550

Query: 549 MVSLILVMRTREFYKGDIYKKFREEGMKKEEMVINGGGEVTARESK 594
           +VSL+LVMRTREFY+GDIYK+FREE +K+E       GE  A ESK
Sbjct: 551 LVSLVLVMRTREFYRGDIYKRFREE-VKQE----TSAGEAAAVESK 590

[Close]

BLAST of Chy12G214320 vs. NCBI nr
Match: XP_023519124.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 946 bits (2444), Expect = 0.0
Identity = 488/586 (83.28%), Postives = 528/586 (90.10%), Query Frame = 0


Query: 9   SDAGHPCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNL 68
           +DAG     LQF L V+ GRWFTVFATFLIMAGAGATYLFGVYSKQIKS LGYDQTTLNL
Sbjct: 11  ADAGRGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNL 70

Query: 69  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCL 128
           MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAKPKVWQMCL
Sbjct: 71  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQMCL 130

Query: 129 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATA 188
           YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIY AVY +DATA
Sbjct: 131 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATA 190

Query: 189 LILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQ 248
           LILLI WLPAA+SVVF+F IR+L++ERQPNE RVFYHFLYISIGLA+FIM+MNI+QKKV+
Sbjct: 191 LILLIAWLPAALSVVFIFNIRKLKAERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVE 250

Query: 249 FNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEES 308
           FNHTAYA SATVICA LFLPL IVIREEL IWN+K +  +PIE+P      EPKI  EES
Sbjct: 251 FNHTAYALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENPI-----EPKISIEES 310

Query: 309 KQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLT 368
           K+I    +     PPP SCFS+IC KP RGDDYTILQALLSIDM VLF+ATFCGLGTSLT
Sbjct: 311 KEIKNNPDPKPYPPPP-SCFSSICSKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLT 370

Query: 369 AVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPRPLMMTLVL 428
           AVDNLGQIGESLGYPLKTV SFVSLVSIWNYFGR+F+GFVSE+LLAKFKFPRPLMMTLVL
Sbjct: 371 AVDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVL 430

Query: 429 LLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 488
           LLSC+GQLLIAFP+PGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS
Sbjct: 431 LLSCIGQLLIAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIAS 490

Query: 489 PLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGA 548
           PLGSYILNVKVAGM+YD EALKQLKE G+DR+AVKEL C+GK+CYRKSF++ AIVTFVGA
Sbjct: 491 PLGSYILNVKVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKRCYRKSFTLMAIVTFVGA 550

Query: 549 MVSLILVMRTREFYKGDIYKKFREEGMKKEEMVINGGGEVTARESK 594
           +VSL+LVMRTREFY+GDIYK+FREE +K+E       GE  A ESK
Sbjct: 551 LVSLVLVMRTREFYRGDIYKRFREE-VKQE----TSAGEAAAVESK 585

[Close]

BLAST of Chy12G214320 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 780.8 bits (2015), Expect = 7.9e-226
Identity = 395/564 (70.04%), Postives = 460/564 (81.56%), Query Frame = 0


Query: 18  LQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 77
           ++F +    GRWF VFA+FLIMA AGATYLFG YSK IKS LGYDQTTLNL+GFFKDLGA
Sbjct: 7   VRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGA 66

Query: 78  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQ 137
           NVGVLSGL+AEVTPTWFVL +GSA+NF GYFMIWL V+G++AKPKVWQMCLYIC+GANSQ
Sbjct: 67  NVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQ 126

Query: 138 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLP 197
           NFANTGALVTC++NFPESRG M+GLLKG+ GLSGAI T++Y A+Y  D+ +LILLI WLP
Sbjct: 127 NFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLP 186

Query: 198 AAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASS 257
           AA+S+VFV+ IR  +  RQ NE  VFY FLYISI LA+F+M MNI +K+V F+  AYA+S
Sbjct: 187 AAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAAS 246

Query: 258 ATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEESKQITEIQNQ 317
           AT+ CA LF+PL + +++EL +WN  K   +PIE P    +++PK   E      +    
Sbjct: 247 ATICCALLFVPLTVSVKQELEVWNMMK---LPIEEPSEVKVEKPK--KELDLDQDKAAKV 306

Query: 318 NAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVDNLGQIG 377
           N      +SCFS +   PPRG+DYTILQALLS DM +LFVATFCGLG+SLTAVDNLGQIG
Sbjct: 307 NGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIG 366

Query: 378 ESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPRPLMMTLVLLLSCVGQLL 437
           ESLGYP  TVSSFVSLVSIWNYFGR+F+GFVSE LLAK+K PRPLMMTLVLLLSC G LL
Sbjct: 367 ESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLL 426

Query: 438 IAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNV 497
           IAFPVPGSVYIAS+++GFSFGAQLPLLFAIISELFGLKY+STLFNCGQ+ASPLGSYILNV
Sbjct: 427 IAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNV 486

Query: 498 KVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVSLILVMR 557
           +V GMLYD EALKQL  +GL R  VK+L C+G QCY+  F I A VTF GA+VSL L +R
Sbjct: 487 RVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIR 546

Query: 558 TREFYKGDIYKKFREEGMKKEEMV 582
           TREFYKGDIYKKFRE    + E+V
Sbjct: 547 TREFYKGDIYKKFRESPESESELV 565

[Close]

BLAST of Chy12G214320 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 673.7 bits (1737), Expect = 1.4e-193
Identity = 343/585 (58.63%), Postives = 444/585 (75.90%), Query Frame = 0


Query: 22  LQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGANVGV 81
           +Q++TGRWF  F + LIM+ AGATY+FG+YS  IK  LGYDQTTLNL+ FFKDLGANVGV
Sbjct: 15  IQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGV 74

Query: 82  LSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQNFAN 141
           L+GL+ EVTP WF+LL+G+ LNF GYFMIWLAV+ RI+KP+VW MCLYICVGANSQ+FAN
Sbjct: 75  LAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFAN 134

Query: 142 TGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLPAAIS 201
           TG+LVTC++NFPESRG ++G+LKG+ GLSGAI+T++YRA Y +D   LIL+IGWLPA +S
Sbjct: 135 TGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVS 194

Query: 202 VVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASSATVI 261
             F+ TIR ++ +RQ NE +VFY+FLYIS+GLA F+M++ I+ K   F  + +  SA V+
Sbjct: 195 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 254

Query: 262 CAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPI--DEPKIIT--------EESKQI 321
              L LP+++VI EE ++W  K+   V +  P P  +  ++PK+ +        EESK++
Sbjct: 255 IVLLLLPIIVVILEEKKLWKEKQ---VALNDPAPINVVTEKPKLDSSEFKDDDGEESKEV 314

Query: 322 TEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVD 381
            E        P   SC++ + + P RGDDYTILQAL S+DM +LF+AT CG+G +LTA+D
Sbjct: 315 VE----KVKTP---SCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAID 374

Query: 382 NLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPRPLMMTLVLLLS 441
           NLGQIG SLGYP ++VS+FVSLVSIWNY+GR+ +G VSE  L K+KFPRPLM+T+VLLLS
Sbjct: 375 NLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLS 434

Query: 442 CVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLG 501
           C G LLIAF VPG +Y+ASVIIGF FGAQ PLLFAIISE+FGLKY+STL+N G +ASP+G
Sbjct: 435 CAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIG 494

Query: 502 SYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVS 561
           SY+LNV+VAG LYD+EA KQ K  G  R   ++L C+G  C++ SF I A VT  G +VS
Sbjct: 495 SYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVS 554

Query: 562 LILVMRTREFYKGDIYKKFREEGMKKE-EMVINGGGEVT-ARESK 595
           ++LV+RT++FYK DIYKKFRE+ +  E EM        T A+E K
Sbjct: 555 MVLVIRTKKFYKSDIYKKFREKALAAEMEMAAPAAARSTVAKEDK 589

[Close]

BLAST of Chy12G214320 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 325.9 bits (834), Expect = 6.9e-89
Identity = 200/571 (35.03%), Postives = 309/571 (54.12%), Query Frame = 0


Query: 25  ITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGANVGVLSG 84
           I  RW    A   I + AG  YLFG  S  IKS+L Y+Q  L+ +G  KDLG +VG ++G
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAG 72

Query: 85  LVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQNFANTGA 144
            ++E+ P W  LL+G+  N  GY  +WL V+GR     +W MC+ I VG N + + NTGA
Sbjct: 73  TLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGA 132

Query: 145 LVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLPAAISVVF 204
           LV+ +QNFP+SRG ++G+LKGF GL GAI+++IY  +++ +  +LIL++   PA + V  
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCL 192

Query: 205 VFTIRRLRSERQ--PNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASSATVIC 264
           +F IR +   +Q  P +   F     + + LA ++M + ++Q  V  +H        V+ 
Sbjct: 193 MFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLF 252

Query: 265 AFLFLPLLIVI-----REELRIWNTKKSTSVPIESPQPKPIDEPKII------------- 324
             L +P+L+ I      E     +T +   VP    Q   +  P +I             
Sbjct: 253 VILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVD 312

Query: 325 ----TEESKQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATF 384
               +E  K+I  +Q Q   A    +   N    P RG+D+T+ QAL+  D +++F +  
Sbjct: 313 LLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLL 372

Query: 385 CGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPR 444
            G G+ LT +DNLGQ+ +SLGY    V   VS++SIWN+ GRI  G+ SE ++  + +PR
Sbjct: 373 LGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYFSELVVRDYAYPR 432

Query: 445 PLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTL 504
           P+ M +  L+  VG +  A+  PG++YI +++IG  +GA   ++ A  SELFGLK F  L
Sbjct: 433 PVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGAL 492

Query: 505 FNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSIT 564
           +N   +A+P GS + +  +A  +YD EA +Q      D      L C G  C+  +  I 
Sbjct: 493 YNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPD--DALRCNGSICFFLTSLIM 552

Query: 565 AIVTFVGAMVSLILVMRTREFYKGDIYKKFR 572
           +    +  M+S+ILV RT+  Y   +Y K R
Sbjct: 553 SGFCIIACMLSMILVRRTKSVYT-HLYGKTR 578

[Close]

BLAST of Chy12G214320 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 1.3e-87
Identity = 197/577 (34.14%), Postives = 313/577 (54.25%), Query Frame = 0


Query: 25  ITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGANVGVLSG 84
           I  RW    A   I + AG  YLFG  S  IKS+L Y+Q  L+ +G  KDLG +VG L+G
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAG 72

Query: 85  LVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQNFANTGA 144
            ++E+ P W  LL+GS  N  GY  +WL V+GR     +W MC+ I VG N + + NT A
Sbjct: 73  TLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAA 132

Query: 145 LVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLPAAISVVF 204
           LV+ +QNFP+SRG ++G+LKGF GL GAIL+++Y  +++ D  +LI ++   P+ + V  
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPL 192

Query: 205 VFTIRRLRSERQ--PNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYASSATVIC 264
           +F IR +   RQ   ++   F     + I LA ++M + +V+  +  +H+   +   V+ 
Sbjct: 193 MFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFTVVLF 252

Query: 265 AFLFLPLLIVIREELRIWNTKKSTSV----------------------------PIESPQ 324
           A L +P+ I I       +T    ++                             +E  +
Sbjct: 253 AILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEK 312

Query: 325 PKPIDEPKIITEESKQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFV 384
           PK +D    + E  K+I ++Q +   A    +        P RG+D+T+ QAL+  D ++
Sbjct: 313 PKEVDLLPAV-ERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWL 372

Query: 385 LFVATFCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLA 444
           +F +   G G+ LT +DNLGQ+ +SLGY    V  FVS++SIWN+ GRI  G+ SE ++ 
Sbjct: 373 IFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--FVSMISIWNFLGRIGGGYFSELIVR 432

Query: 445 KFKFPRPLMMTLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGL 504
            + +PRP+ + +  L+  VG +  A+  PG+++I +++IG  +GA   ++ A  SELFGL
Sbjct: 433 DYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFGL 492

Query: 505 KYFSTLFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYR 564
           K F  L+N   +A+P GS + +  +A  +YD EA +Q +    +   V  L C G  CY 
Sbjct: 493 KKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDV--LRCRGSICYF 552

Query: 565 KSFSITAIVTFVGAMVSLILVMRTREFYKGDIYKKFR 572
            +  I +    + A +S+ILV RT+  Y  ++Y K R
Sbjct: 553 LTSLIMSGFCLIAAALSMILVQRTKPVYT-NLYGKTR 583

[Close]

BLAST of Chy12G214320 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 316.6 bits (810), Expect = 4.2e-86
Identity = 192/564 (34.04%), Postives = 316/564 (56.03%), Query Frame = 0


Query: 22  LQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGANVGV 81
           ++++  +W  + A+  I   +GA+Y FG+YS  +KS   YDQ+TL+ +  FKD+GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 82  LSGLVAEVTPT----------------WFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQ 141
            SGL+     +                W VL +G+   F GYF+IW +V+G I KP V  
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 142 MCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADD 201
           MCL++ + A SQ F NT  +V+ ++NF +  G  +G++KGF GLSGAIL ++Y  + A D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 202 ATALILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQK 261
             + ILL+   P  +S++ V  + R+      ++K+       +S+ +A ++MI+ I+  
Sbjct: 181 PASFILLLAVTPTVLSLL-VMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIIL-- 240

Query: 262 KVQFNHTAYASSATVICAFLFLPLLIVIREELRIWNTKKSTSVPIESPQPKPIDEPKIIT 321
           K  F  +++A+  T++C  + L L ++I    +    +K+           P D   +I+
Sbjct: 241 KNTFGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTV----------PHDYSPLIS 300

Query: 322 EESKQITEIQNQNAAAPPPESCFSNICHKPPRGDDYTILQALLSIDMFVLFVATFCGLGT 381
             S + T   NQ++         S +  +    ++  +LQA+  +  ++LF+A  CG+G+
Sbjct: 301 --SPKATTSGNQSSEGD------SKV--EAGLSENLNLLQAMKKLSFWLLFLAMICGMGS 360

Query: 382 SLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLAKFKFPRPLMMT 441
            L+ ++N+ QIGESL Y    ++S VSL SIWN+ GR  AG+ S++LL K  +PRPL+M 
Sbjct: 361 GLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMA 420

Query: 442 LVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQ 501
             L    +G L+IA    G++Y+ SVI+G  +G+Q  L+  I SELFG+++  T+FN   
Sbjct: 421 ATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTIS 480

Query: 502 IASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTF 561
           +ASP+GSYI +V++ G +YD  A            + +   C G  C+R SF I A V F
Sbjct: 481 VASPIGSYIFSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAF 529

Query: 562 VGAMVSLILVMRTREFYKGDIYKK 570
            G +V+++L  RT+  Y+  + K+
Sbjct: 541 FGFLVAIVLFFRTKTLYRQILVKR 529

The following BLAST results are available for this feature:

BLAST of Chy12G214320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	6.4e-31	25.68	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]

BLAST of Chy12G214320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LXR5	0.0e+00	98.32	Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G09765...	[more]
A0A5A7UED5	2.2e-310	92.40	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CP80	2.2e-310	92.40	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103503227 ...	[more]
A0A6J1HPV2	2.4e-270	84.06	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1I026	4.0e-270	82.76	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469...	[more]

BLAST of Chy12G214320 vs. NCBI nr


Match Name	Identity	Description
XP_004147280.1	98.32	protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN64786.1 hypothetical pr...	[more]
XP_008465591.1	92.40	PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0051859.1 ...	[more]
XP_038895725.1	87.43	protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida]	[more]
XP_023519125.1	83.45	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023519124.1	83.28	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy12G214320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28120.1	7.9e-226	70.04	Major facilitator superfamily protein	[more]
AT2G39210.1	1.4e-193	58.63	Major facilitator superfamily protein	[more]
AT5G14120.1	6.9e-89	35.03	Major facilitator superfamily protein	[more]
AT3G01930.2	1.3e-87	34.14	Major facilitator superfamily protein	[more]
AT1G74780.1	4.2e-86	34.04	Nodulin-like / Major Facilitator Superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 347..563 e-value: 5.2E-10 score: 41.0
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 28..560
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 28..273 e-value: 1.6E-92 score: 309.5
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 16..579
None	No IPR available	PANTHER	PTHR21576:SF29	PROTEIN NUCLEAR FUSION DEFECTIVE 4	coord: 16..579
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 27..548 e-value: 4.08812E-106 score: 323.049

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G214320.1	Chy12G214320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006817	phosphate ion transport
biological_process	GO:0050896	response to stimulus
cellular_component	GO:0016021	integral component of membrane