Chy12G214190 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G214190
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	iron-sulfur assembly protein IscA-like 2, mitochondrial
Location	chrH12: 13287690 .. 13289734 (+)
RNA-Seq Expression	Chy12G214190
Synteny	Chy12G214190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTCCCAGATTTCTAATTCAGCGGCTAACACCATATTTGGGAGCACGTATCCTAAAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCCTATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTGCGGGCATTGATACAATTCACATGACAGATAACTGCATACGGGTATCCCTTATCTTTATGTAATCCTTTTTGTGCATTTACCATATGGATTTAAAATTATTCATCTATTTCACTGTCATTATTGTTTTAGTTTTTGATTTTTTGTGCTATCCTCATTTTGTGCATTATTTGGACCTATAATTTCTTGGTTGTAGGATGCTAATGAAGTATTGCCTTGAACACGATTAAAATATGAGTGAAGAATTTGAGATTTGGGCATTTTCTTGTTGTCGCTTCAAATTTGGGCATTTGCTTTGTCATACGTGATGCCCTAAAGCGAGGCTTAATTTATGATACCAAAACTGAAGTATTAAGAAGAATATATGGTGTCAGTTATAATAATTGTCTTATTCTTAATTTTTTAAAGAACTTTAACTAAATTAAAGGAAGTGAGACTTATAAACTTAGGAATGAACTCTTCACAACTAGATGTATGATGTATTATCTGTTACCAAAGGGCTAAATTGGTTAATACTCGTAATTATGTTATTCATCATGGTTGACATAGTCCTTGATCTTCATCCTTTAAAAGCATTCTTACCATCATTTTTCTACACATTTTTTTCCCTGCTGCATTAAAGTAAGTTTTGAATAATGACTTTCTGCTAAGTACTGAGATGCCCTTGCAGAGGATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGGTTGAGTGTAGAAAATGGGGGATGTTCTGGGTTTCAATATGTTTTCAATTTGGATGACAAAACCAATCCAGATGACAGGTCTATTAAATAACTTATACTCACTTCTTAATTTGGATTTTCACCTAAAGATTGCCGATGATGTTTGCTTTCACTCTTTAAAACTTTACCCTTTTCCCTCCTTTTATGTTTGTAGGGTGTGTGAGAAGGGAGGAGTTAAATTGGTAGTTGATATCATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAGGAGCTGATTCGTTCCGCTTTCGTTGTGAGTAAAACCGATGTCTTACCATTTGCTGGATCACCATTGTTGCTTAGATATGTTTATTATTATTTGGGAAAAATATTTGGTCCTAGATTTGGGTCTCTTCCATGTTGGTTCTTAAGTTTCAAAATGTTACACTTTTAGTCAATAAGTTCTACTTTGATTTTAATTTAGCCTTTAGATTTCAAAATATTATGCTTTTAGACCTTAAAATGTGAATTTTGTTTTAATCTAGTCACAGGTCTCAAGATTTACACTTTTAACCTCAATTTTGTTTTAATCTAGTCACAGGTCTCAAGAAACAAAACTCAAACCCTTAATCATTAACAGCTTTTGTCCATTTAGTTTTTTTTTTTTTTTAAATCAAGTTTATTAACATTTCTTCCTTTTATTACTTTCTATGCTTTGTTGTCTCAAAATCGTAGCTAAAATCTGAAAACTAAAAAGGTAAATTTCAATCGCTCGTTTTTTTTTCTAGAAATTGACTAAAGAACTTTATGGGCGTTTGGGACAAGGTGACCACAAATCCAAGTGATGTTGGAGGGAGGCTATGGTTTTTAAAGTACTTTCAAAATTTGCTATAACATATAAATTGTTTAGTCCATTTTTCTATATATAAAAAGCCCCAAACAAAGAAAGACTAAGTAGTTACTAAACGAGGTTTAAAAGGGTAACATTTTGAATTCTAAAAGTTGAACAGAAACTAAATCCAAAGCTTGGAGACCAAAAAACATATATAACTCATTTTACATCATATGTTTTATGCATTCTTATTTCTTATTTCACAACAAAAGTAGTGCTAATGCAGAAGTTGAATTCTTGTAATATGCCATACGCAGGTGACCACAAATCCAAGTGCTGTTGGTGGCTGTAGTTGTAAAAGTTCTTTTATGGTGAAACAATAA

mRNA sequence

ATGGTTCCCAGATTTCTAATTCAGCGGCTAACACCATATTTGGGAGCACGTATCCTAAAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCCTATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTGCGGGCATTGATACAATTCACATGACAGATAACTGCATACGGAGGATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGGTTGAGTGTAGAAAATGGGGGATGTTCTGGGTTTCAATATGTTTTCAATTTGGATGACAAAACCAATCCAGATGACAGGGTGTGTGAGAAGGGAGGAGTTAAATTGGTAGTTGATATCATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAGGAGCTGATTCGTTCCGCTTTCGTTGTGACCACAAATCCAAGTGCTGTTGGTGGCTGTAGTTGTAAAAGTTCTTTTATGGTGAAACAATAA

Coding sequence (CDS)

ATGGTTCCCAGATTTCTAATTCAGCGGCTAACACCATATTTGGGAGCACGTATCCTAAAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCCTATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTGCGGGCATTGATACAATTCACATGACAGATAACTGCATACGGAGGATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGGTTGAGTGTAGAAAATGGGGGATGTTCTGGGTTTCAATATGTTTTCAATTTGGATGACAAAACCAATCCAGATGACAGGGTGTGTGAGAAGGGAGGAGTTAAATTGGTAGTTGATATCATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAGGAGCTGATTCGTTCCGCTTTCGTTGTGACCACAAATCCAAGTGCTGTTGGTGGCTGTAGTTGTAAAAGTTCTTTTATGGTGAAACAATAA

Protein sequence

MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMTDNCIRRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ*

Homology

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BLAST of Chy12G214190 vs. ExPASy Swiss-Prot
Match: Q8LCY2 (Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g03905 PE=1 SV=2)

HSP 1 Score: 201.4 bits (511), Expect = 7.3e-51
Identity = 109/156 (69.87%), Postives = 125/156 (80.13%), Query Frame = 0


Query: 4   RFLIQRLTPYLGARILKNQRLLS-SSVSAYQEASSSLIT--PSAGIDTIHMTDNCIRRMK 63
           R L++R+ PYL  RI +N RLL+ SS SA +EASSS  +   S+  D +H++DNCIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQ--EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVK 123
           ELQ  EP E+KMLRL VE GGCSGFQY F LD++TNPDDRV EK GVKLVVD +SYD VK
Sbjct: 63  ELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVK 122

Query: 124 GATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVK 155
           GATIDY EELIR+AFVV  NPSAVGGCSCKSSFMVK
Sbjct: 123 GATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

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BLAST of Chy12G214190 vs. ExPASy Swiss-Prot
Match: Q2TBG7 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 3.7e-31
Identity = 72/135 (53.33%), Postives = 89/135 (65.93%), Query Frame = 0


Query: 20  KNQRLLSSSVSAYQEASSSLITPSAGIDTIHMTDNCIRRMKELQEPMEEKMLRLSVENGG 79
           +  RLL++S         S  +P AG   IH+TD+C++R+ E+ E  E   LRL VE GG
Sbjct: 19  QRSRLLAASRGPRALREVSFSSPEAGEGQIHLTDSCVQRLLEITEGSE--FLRLEVEGGG 78

Query: 80  CSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVKGATIDYVEELIRSAFVVTTNP 139
           CSGFQY F+LD   NPDDRV EKGG ++VVD  S  FVKGA +D+ +ELIRS+F V  NP
Sbjct: 79  CSGFQYKFSLDTVINPDDRVFEKGGARVVVDSDSLAFVKGAHVDFSQELIRSSFQVLNNP 138

Query: 140 SAVGGCSCKSSFMVK 155
            A  GCSC SSF VK
Sbjct: 139 QAQQGCSCGSSFSVK 151

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BLAST of Chy12G214190 vs. ExPASy Swiss-Prot
Match: Q86U28 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN=ISCA2 PE=1 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 8.4e-31
Identity = 74/134 (55.22%), Postives = 94/134 (70.15%), Query Frame = 0


Query: 23  RLLSSSV--SAYQEASSSLITPSAGIDTIHMTDNCIRRMKELQEPMEEKMLRLSVENGGC 82
           RLL++S+   A +EASSS  +P AG   I +TD+C++R+ E+ E  E   LRL VE GGC
Sbjct: 24  RLLTASLGPQARREASSS--SPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGC 83

Query: 83  SGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVKGATIDYVEELIRSAFVVTTNPS 142
           SGFQY F+LD   NPDDRV E+GG ++VVD  S  FVKGA +D+ +ELIRS+F V  NP 
Sbjct: 84  SGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQ 143

Query: 143 AVGGCSCKSSFMVK 155
           A  GCSC SSF +K
Sbjct: 144 AQQGCSCGSSFSIK 153

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BLAST of Chy12G214190 vs. ExPASy Swiss-Prot
Match: Q5R788 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 8.4e-31
Identity = 74/134 (55.22%), Postives = 94/134 (70.15%), Query Frame = 0


Query: 23  RLLSSSV--SAYQEASSSLITPSAGIDTIHMTDNCIRRMKELQEPMEEKMLRLSVENGGC 82
           RLL++S+   A +EASSS  +P AG   I +TD+C++R+ E+ E  E   LRL VE GGC
Sbjct: 24  RLLAASLGPQARREASSS--SPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGC 83

Query: 83  SGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVKGATIDYVEELIRSAFVVTTNPS 142
           SGFQY F+LD   NPDDRV E+GG ++VVD  S  FVKGA +D+ +ELIRS+F V  NP 
Sbjct: 84  SGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQ 143

Query: 143 AVGGCSCKSSFMVK 155
           A  GCSC SSF +K
Sbjct: 144 AQQGCSCGSSFSIK 153

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BLAST of Chy12G214190 vs. ExPASy Swiss-Prot
Match: Q9DCB8 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 GN=Isca2 PE=1 SV=2)

HSP 1 Score: 129.8 bits (325), Expect = 2.7e-29
Identity = 68/121 (56.20%), Postives = 84/121 (69.42%), Query Frame = 0


Query: 34  EASSSLITPSAGIDTIHMTDNCIRRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKT 93
           E +SS+  P AG   I +TD+C++R+ E+ E  E   LRL VE GGCSGFQY F+LD   
Sbjct: 37  ETTSSI--PEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQYKFSLDTVI 96

Query: 94  NPDDRVCEKGGVKLVVDIISYDFVKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMV 153
           NPDDRV E+GG ++VVD  S  FVKGA +D+ +ELIRS+F V  NP A  GCSC SSF V
Sbjct: 97  NPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSV 153

Query: 154 K 155
           K
Sbjct: 157 K 153

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BLAST of Chy12G214190 vs. ExPASy TrEMBL
Match: A0A6J1KFL9 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111493424 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.3e-66
Identity = 136/159 (85.53%), Postives = 140/159 (88.05%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLI----TPSAGIDTIHMTDNCI 60
           MVPR L QRL PYL ARI +NQRLLSSSVSA QEASS L     +PS   DTIHMTDNCI
Sbjct: 1   MVPRLLSQRLAPYLAARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDF 120
           RRMKELQEPMEEKMLRLSVE GGCSGFQYVFNLDD+TNPDDRVCEK GVKLVVD ISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 156
           VKGAT+DYVEELIR AFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G214190 vs. ExPASy TrEMBL
Match: A0A6J1GA98 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111452373 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.2e-66
Identity = 136/159 (85.53%), Postives = 140/159 (88.05%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLI----TPSAGIDTIHMTDNCI 60
           MVPR L QRL PYL ARI +NQRLLSSSVSA QEASS L     +PS   DTIHMTDNCI
Sbjct: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDF 120
           RRMKELQEPMEEKMLRLSVE GGCSGFQYVFNLDD+TNPDDRVCEK GVKLVVD ISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 156
           VKGAT+DYVEELIR AFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G214190 vs. ExPASy TrEMBL
Match: A0A6J1DIX6 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111020448 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 3.2e-65
Identity = 134/159 (84.28%), Postives = 140/159 (88.05%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLITPSAG----IDTIHMTDNCI 60
           MVPR LIQRLTP+  ARI +NQRLLSSSVSA+QE  S L +PS      IDTIHMTDNCI
Sbjct: 1   MVPRLLIQRLTPHFIARIRENQRLLSSSVSAHQEVPSLLTSPSPSSSVLIDTIHMTDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDF 120
           ++MKELQEPMEEKMLRLSVE GGCSGFQYVFNLDDKTNPDDRV EK GVKLVVD ISYDF
Sbjct: 61  QKMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDKTNPDDRVYEKDGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 156
           VKGATIDY EELIRSAFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYAEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G214190 vs. ExPASy TrEMBL
Match: A0A0A0LSN2 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G108280 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 6.0e-64
Identity = 128/134 (95.52%), Postives = 130/134 (97.01%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMTDNCIRRMK 60
           MVPRFLIQRLTPYLGA I KNQRLLSSSVSAYQEASSSLITPSAGIDTIHM+DNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEK GVKLVVD ISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFV 135
           TIDYVEELIRSAF+
Sbjct: 121 TIDYVEELIRSAFI 134

[Close]

BLAST of Chy12G214190 vs. ExPASy TrEMBL
Match: A0A6J1HPD1 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464825 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 6.0e-64
Identity = 133/159 (83.65%), Postives = 140/159 (88.05%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLITPSAG----IDTIHMTDNCI 60
           M PR LIQRLTP+L ARI +NQRLLSSSVSA+QEASS L +PS      IDTI MTDNCI
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDF 120
           RRMKELQ P EEKMLRLSVE GGCSGFQYVF+LDDKTNPDDRV EK GV+LVVD ISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 156
           VKGATIDYVEELIRSAFVV+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G214190 vs. NCBI nr
Match: XP_004147288.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Cucumis sativus] >KGN64807.2 hypothetical protein Csa_013472 [Cucumis sativus])

HSP 1 Score: 295 bits (756), Expect = 1.33e-100
Identity = 149/155 (96.13%), Postives = 151/155 (97.42%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMTDNCIRRMK 60
           MVPRFLIQRLTPYLGA I KNQRLLSSSVSAYQEASSSLITPSAGIDTIHM+DNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEK GVKLVVD ISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 155
           TIDYVEELIRSAF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 TIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 155

[Close]

BLAST of Chy12G214190 vs. NCBI nr
Match: XP_038895338.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Benincasa hispida] >XP_038895339.1 iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 283 bits (723), Expect = 1.43e-95
Identity = 143/155 (92.26%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMTDNCIRRMK 60
           MVPRFLIQRLTPY  ARI +NQRLLSSSV A+QE SSSLITPS GIDTIHMTDNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYFAARIRENQRLLSSSVLAHQETSSSLITPSVGIDTIHMTDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVKGA 120
           ELQEPMEEKMLRLSVE GGCSGFQYVFNLD+KTNPDDRVCEK GVKLVVD ISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVETGGCSGFQYVFNLDEKTNPDDRVCEKQGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 155
           TIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 TIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 155

[Close]

BLAST of Chy12G214190 vs. NCBI nr
Match: XP_038895337.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 283 bits (723), Expect = 3.60e-95
Identity = 143/155 (92.26%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMTDNCIRRMK 60
           MVPRFLIQRLTPY  ARI +NQRLLSSSV A+QE SSSLITPS GIDTIHMTDNCIRRMK
Sbjct: 28  MVPRFLIQRLTPYFAARIRENQRLLSSSVLAHQETSSSLITPSVGIDTIHMTDNCIRRMK 87

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVKGA 120
           ELQEPMEEKMLRLSVE GGCSGFQYVFNLD+KTNPDDRVCEK GVKLVVD ISYDFVKGA
Sbjct: 88  ELQEPMEEKMLRLSVETGGCSGFQYVFNLDEKTNPDDRVCEKQGVKLVVDNISYDFVKGA 147

Query: 121 TIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 155
           TIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 148 TIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 182

[Close]

BLAST of Chy12G214190 vs. NCBI nr
Match: XP_011653808.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 266 bits (681), Expect = 2.85e-89
Identity = 136/144 (94.44%), Postives = 138/144 (95.83%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMTDNCIRRMK 60
           MVPRFLIQRLTPYLGA I KNQRLLSSSVSAYQEASSSLITPSAGIDTIHM+DNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEK GVKLVVD ISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFVVTTNPSAVGG 144
           TIDYVEELIRSAF+VTTNPS V G
Sbjct: 121 TIDYVEELIRSAFIVTTNPSDVEG 144

[Close]

BLAST of Chy12G214190 vs. NCBI nr
Match: XP_023523533.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 264 bits (674), Expect = 4.83e-88
Identity = 137/159 (86.16%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 1   MVPRFLIQRLTPYLGARILKNQRLLSSSVSAYQEASSSLITPSAG----IDTIHMTDNCI 60
           MVPR L QRL PYL ARI +NQRLLSSSVSA QEASS L +PS      IDTIHMTDNCI
Sbjct: 1   MVPRLLSQRLAPYLAARIRENQRLLSSSVSALQEASSLLTSPSQSPSPDIDTIHMTDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDF 120
           RRMKELQEPMEEKMLRLSVE GGCSGFQYVFNLDD+TNPDDRVCEK GVKLVVD ISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 155
           VKGAT+DYVEELIR AFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G214190 vs. TAIR 10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 201.4 bits (511), Expect = 5.2e-52
Identity = 109/156 (69.87%), Postives = 125/156 (80.13%), Query Frame = 0


Query: 4   RFLIQRLTPYLGARILKNQRLLS-SSVSAYQEASSSLIT--PSAGIDTIHMTDNCIRRMK 63
           R L++R+ PYL  RI +N RLL+ SS SA +EASSS  +   S+  D +H++DNCIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQ--EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVDIISYDFVK 123
           ELQ  EP E+KMLRL VE GGCSGFQY F LD++TNPDDRV EK GVKLVVD +SYD VK
Sbjct: 63  ELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVK 122

Query: 124 GATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVK 155
           GATIDY EELIR+AFVV  NPSAVGGCSCKSSFMVK
Sbjct: 123 GATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

[Close]

BLAST of Chy12G214190 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 71.6 bits (174), Expect = 6.2e-13
Identity = 37/118 (31.36%), Postives = 65/118 (55.08%), Query Frame = 0


Query: 35  ASSSLITPSAGIDTIHMTDNCIRRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTN 94
           A+++ + P+     + +TD    R+  L +  ++  LRL V+  GC+G  Y  N  D+  
Sbjct: 9   AAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYADEKG 68

Query: 95  PDDRVCEKGGVKLVVDIISYDFVKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFM 153
             D + E+ GV+++V+  +   V G  +D+V++ +RS FV   NP++ G C C  SFM
Sbjct: 69  KFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVF-INPNSQGQCGCGESFM 125

[Close]

BLAST of Chy12G214190 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 68.2 bits (165), Expect = 6.8e-12
Identity = 37/101 (36.63%), Postives = 58/101 (57.43%), Query Frame = 0


Query: 51  MTDNCIRRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKGGVKLVVD 110
           ++D    R+++L +  ++  LRL+VE  GC+G  YV N   +    D V E+ GVK++VD
Sbjct: 8   LSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVD 67

Query: 111 IISYDFVKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSF 152
             +   V G  +D+V++ +RS FV   NP+A   C C  SF
Sbjct: 68  PKAVMHVIGTEMDFVDDKLRSEFVF-VNPNAT-KCGCGESF 106

[Close]

BLAST of Chy12G214190 vs. TAIR 10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 67.8 bits (164), Expect = 8.9e-12
Identity = 38/124 (30.65%), Postives = 67/124 (54.03%), Query Frame = 0


Query: 35  ASSSLITPSAGIDTIHMTDNCIRRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTN 94
           A+++ + P+     + +TD    R+  L +  ++  LRL V+  GC+G  Y  N   KT+
Sbjct: 9   AAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYAGKTS 68

Query: 95  PD------DRVCEKGGVKLVVDIISYDFVKGATIDYVEELIRSAFVVTTNPSAVGGCSCK 153
            +      D + E+ GV+++V+  +   V G  +D+V++ +RS FV   NP++ G C C 
Sbjct: 69  GNDEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVF-INPNSQGQCGCG 128

[Close]

BLAST of Chy12G214190 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 65.1 bits (157), Expect = 5.8e-11
Identity = 42/142 (29.58%), Postives = 70/142 (49.30%), Query Frame = 0


Query: 13  YLGARILKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMTDNCIRRMKELQ-EPMEEKML 72
           Y+   + +  RL   S S     +   + P+     I +++N ++ + +++ E  E+  L
Sbjct: 41  YVNLNLNRRNRLSVRSASVPAAPAMEGLKPA-----ISLSENALKHLSKMRSERGEDLCL 100

Query: 73  RLSVENGGCSGFQYVFNLDDKTN--PDDRVCEKGGVKLVVDIISYDFVKGATIDYVEELI 132
           R+ V+ GGCSG  Y  + +++ N  PDD   E  G  +V D  S  F+ G  +DY + LI
Sbjct: 101 RIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSDALI 160

Query: 133 RSAFVVTTNPSAVGGCSCKSSF 152
              F   +NP+A   C C  SF
Sbjct: 161 GGGFSF-SNPNATQTCGCGKSF 176

The following BLAST results are available for this feature:

BLAST of Chy12G214190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LCY2	7.3e-51	69.87	Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana ...	[more]
Q2TBG7	3.7e-31	53.33	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=I...	[more]
Q86U28	8.4e-31	55.22	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN...	[more]
Q5R788	8.4e-31	55.22	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN...	[more]
Q9DCB8	2.7e-29	56.20	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 G...	[more]

BLAST of Chy12G214190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KFL9	1.3e-66	85.53	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3...	[more]
A0A6J1GA98	2.2e-66	85.53	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX...	[more]
A0A6J1DIX6	3.2e-65	84.28	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia O...	[more]
A0A0A0LSN2	6.0e-64	95.52	Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G108280...	[more]
A0A6J1HPD1	6.0e-64	83.65	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX...	[more]

BLAST of Chy12G214190 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147288.1	1.33e-100	96.13	iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Cucumis sati...	[more]
XP_038895338.1	1.43e-95	92.26	iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Benincasa hi...	[more]
XP_038895337.1	3.60e-95	92.26	iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Benincasa hi...	[more]
XP_011653808.1	2.85e-89	94.44	iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Cucumis sati...	[more]
XP_023523533.1	4.83e-88	86.16	iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita pepo subsp. p...	[more]

BLAST of Chy12G214190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03905.1	5.2e-52	69.87	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.1	6.2e-13	31.36	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	6.8e-12	36.63	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.3	8.9e-12	30.65	Iron-sulphur cluster biosynthesis family protein	[more]
AT1G10500.1	5.8e-11	29.58	chloroplast-localized ISCA-like protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 49..153 e-value: 2.8E-33 score: 112.2
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 49..148 e-value: 3.6E-14 score: 52.9
IPR035903	HesB-like domain superfamily	GENE3D	2.60.300.12		coord: 48..155 e-value: 2.1E-33 score: 116.7
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 48..141
None	No IPR available	PANTHER	PTHR43011	IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL	coord: 4..155
None	No IPR available	PANTHER	PTHR43011:SF4	BNACNNG10620D PROTEIN	coord: 4..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G214190.1	Chy12G214190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0106035	protein maturation by [4Fe-4S] cluster transfer
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding