Chy12G213450 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G213450
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	lysosomal Pro-X carboxypeptidase-like
Location	chrH12: 11434578 .. 11435374 (+)
RNA-Seq Expression	Chy12G213450
Synteny	Chy12G213450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTGCATTGTGAGCCAATCCATATTTCTTCCACTACTACTTCTTTTTATTTCTTCATGTGCTCGTGGCCACATCCCAGTCCTTGGAGTGCAAAGAAGGGCATTCCAAAGTACACCCCAACAATTAGATGGACTTGCAACTTTCTATTACAAACAACCACTTGATCATTTCAATTATCAACCTCAAAGTTACGTTACATTTGATCAAAGATATATCATTGATTTTAAGTATTGGAAAGGTATCAATCCCAAAACTCCTATCTTTGCTTTCCTCGGTGCCGAAAGTGACATTGACAATGACGTACCCTACGTTGGCTTTCCTCTTCGTTTCGCCTCTCAATACAAGGCTATGTCGGTATATTTAGAGGTATGCATGCTAAGTAATATACCGAATAATTAATTTGACCACATTGAAACTACTACTACTACATAGAAATATAAAATTTGATTCATACAAGTTTTTTAATAATTCTCACGTGTGAATAAACTAGCAATAACTTAATATAAAAGTTAAAATGTTAAAATATCATTATAATTATAGTCCCTTTAGTTTGAATTTCATTCTAATTTTATGGTTTATATGACACATATATGTTGTAATTTTATAGCATAGATTTTATGGAAAATCAGTACCATTTGGTTCACTAGAGAAGGCTATGAAAAATGGAAGCATTAGAGGGCATTTTAATTCAGCTCAAGCCTTAGCAGATTATGCTGAATTGCTTTTGCACATCAAGAAAATGTTTGCATATGATACTTCTCCAATTATAGTTATGGGAGCCTCCTATGGTGGAA

mRNA sequence

ATGGACTGCATTGTGAGCCAATCCATATTTCTTCCACTACTACTTCTTTTTATTTCTTCATGTGCTCGTGGCCACATCCCAGTCCTTGGAGTGCAAAGAAGGGCATTCCAAAGTACACCCCAACAATTAGATGGACTTGCAACTTTCTATTACAAACAACCACTTGATCATTTCAATTATCAACCTCAAAGTTACGTTACATTTGATCAAAGATATATCATTGATTTTAAGTATTGGAAAGGTATCAATCCCAAAACTCCTATCTTTGCTTTCCTCGGTGCCGAAAGTGACATTGACAATGACGTACCCTACGTTGGCTTTCCTCTTCGTTTCGCCTCTCAATACAAGGCTATGTCGGTATATTTAGAGCATAGATTTTATGGAAAATCAGTACCATTTGGTTCACTAGAGAAGGCTATGAAAAATGGAAGCATTAGAGGGCATTTTAATTCAGCTCAAGCCTTAGCAGATTATGCTGAATTGCTTTTGCACATCAAGAAAATGTTTGCATATGATACTTCTCCAATTATAGTTATGGGAGCCTCCTATGGTGGAA

Coding sequence (CDS)

ATGGACTGCATTGTGAGCCAATCCATATTTCTTCCACTACTACTTCTTTTTATTTCTTCATGTGCTCGTGGCCACATCCCAGTCCTTGGAGTGCAAAGAAGGGCATTCCAAAGTACACCCCAACAATTAGATGGACTTGCAACTTTCTATTACAAACAACCACTTGATCATTTCAATTATCAACCTCAAAGTTACGTTACATTTGATCAAAGATATATCATTGATTTTAAGTATTGGAAAGGTATCAATCCCAAAACTCCTATCTTTGCTTTCCTCGGTGCCGAAAGTGACATTGACAATGACGTACCCTACGTTGGCTTTCCTCTTCGTTTCGCCTCTCAATACAAGGCTATGTCGGTATATTTAGAGCATAGATTTTATGGAAAATCAGTACCATTTGGTTCACTAGAGAAGGCTATGAAAAATGGAAGCATTAGAGGGCATTTTAATTCAGCTCAAGCCTTAGCAGATTATGCTGAATTGCTTTTGCACATCAAGAAAATGTTTGCATATGATACTTCTCCAATTATAGTTATGGGAGCCTCCTATGGTGGAA

Protein sequence

MDCIVSQSIFLPLLLLFISSCARGHIPVLGVQRRAFQSTPQQLDGLATFYYKQPLDHFNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKAMSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPIIVMGASYGGX

Homology

[Close]

BLAST of Chy12G213450 vs. ExPASy Swiss-Prot
Match: P42785 (Lysosomal Pro-X carboxypeptidase OS=Homo sapiens OX=9606 GN=PRCP PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.0e-19
Identity = 56/149 (37.58%), Postives = 81/149 (54.36%), Query Frame = 0


Query: 38  STPQQLDGLATFYYKQPLDHFNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESD 97
           S P      +  Y++Q +DHF +   +  TF+QRY++  KYWK       I  + G E D
Sbjct: 40  SLPAVAKNYSVLYFQQKVDHFGF--NTVKTFNQRYLVADKYWK--KNGGSILFYTGNEGD 99

Query: 98  IDNDVPYVGFPLRFASQYKAMSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALAD 157
           I       GF    A + KAM V+ EHR+YG+S+PFG  + + K+        S QALAD
Sbjct: 100 IIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFG--DNSFKDSRHLNFLTSEQALAD 159

Query: 158 YAELLLHIKKMF-AYDTSPIIVMGASYGG 186
           +AEL+ H+K+     +  P+I +G SYGG
Sbjct: 160 FAELIKHLKRTIPGAENQPVIAIGGSYGG 182

[Close]

BLAST of Chy12G213450 vs. ExPASy Swiss-Prot
Match: Q5RBU7 (Lysosomal Pro-X carboxypeptidase OS=Pongo abelii OX=9601 GN=PRCP PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.3e-19
Identity = 56/149 (37.58%), Postives = 80/149 (53.69%), Query Frame = 0


Query: 38  STPQQLDGLATFYYKQPLDHFNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESD 97
           S P      +  Y++Q +DHF +   +  TF+QRY++  KYWK       I  + G E D
Sbjct: 40  SLPAVAKNYSVLYFQQKVDHFGF--NTVKTFNQRYLVADKYWK--KNGGSILFYTGNEGD 99

Query: 98  IDNDVPYVGFPLRFASQYKAMSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALAD 157
           I       GF    A + KAM V+ EHR+YG+S+PFG  +   K+        S QALAD
Sbjct: 100 IIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFG--DNTFKDSRHLNFLTSEQALAD 159

Query: 158 YAELLLHIKKMF-AYDTSPIIVMGASYGG 186
           +AEL+ H+K+     +  P+I +G SYGG
Sbjct: 160 FAELIKHLKRTIPGAENQPVIAIGGSYGG 182

[Close]

BLAST of Chy12G213450 vs. ExPASy Swiss-Prot
Match: Q9EPB1 (Dipeptidyl peptidase 2 OS=Rattus norvegicus OX=10116 GN=Dpp7 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 5.1e-19
Identity = 54/139 (38.85%), Postives = 79/139 (56.83%), Query Frame = 0


Query: 50  YYKQPLDHFNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPL 109
           Y++Q +DHFN++  S  TF QR+++  K+WK    + PIF + G E DI +     GF +
Sbjct: 45  YFEQYMDHFNFESFSNKTFGQRFLVSDKFWK--MGEGPIFFYTGNEGDIWSLANNSGFIV 104

Query: 110 RFASQYKAMSVYLEHRFYGKSVPFGSLEKAMKNGSIRGH---FNSAQALADYAELLLHIK 169
             A+Q +A+ V+ EHR+YGKS+PFG         + RG+       QALAD+A LL  ++
Sbjct: 105 ELAAQQEALLVFAEHRYYGKSLPFG------VQSTQRGYTQLLTVEQALADFAVLLQALR 164

Query: 170 KMFAYDTSPIIVMGASYGG 186
                  +P I  G SYGG
Sbjct: 165 HNLGVQDAPTIAFGGSYGG 175

[Close]

BLAST of Chy12G213450 vs. ExPASy Swiss-Prot
Match: Q9ET22 (Dipeptidyl peptidase 2 OS=Mus musculus OX=10090 GN=Dpp7 PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 8.7e-19
Identity = 53/139 (38.13%), Postives = 79/139 (56.83%), Query Frame = 0


Query: 50  YYKQPLDHFNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPL 109
           Y++Q +DHFN++     TF QR+++  K+WK    + PIF + G E DI +     GF +
Sbjct: 45  YFEQYMDHFNFESFGNKTFGQRFLVSDKFWK--MGEGPIFFYTGNEGDIWSFANNSGFMV 104

Query: 110 RFASQYKAMSVYLEHRFYGKSVPFGSLEKAMKNGSIRGH---FNSAQALADYAELLLHIK 169
             A+Q +A+ V+ EHR+YGKS+PFG         + RG+       QALAD+A LL  ++
Sbjct: 105 ELAAQQEALLVFAEHRYYGKSLPFG------VQSTQRGYTQLLTVEQALADFAVLLQALR 164

Query: 170 KMFAYDTSPIIVMGASYGG 186
           +      +P I  G SYGG
Sbjct: 165 QDLGVHDAPTIAFGGSYGG 175

[Close]

BLAST of Chy12G213450 vs. ExPASy Swiss-Prot
Match: Q7TMR0 (Lysosomal Pro-X carboxypeptidase OS=Mus musculus OX=10090 GN=Prcp PE=1 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-17
Identity = 60/180 (33.33%), Postives = 89/180 (49.44%), Query Frame = 0


Query: 9   IFLPLLLLFISSCARGHIPVLGVQRRAFQSTPQQLDG--LATFYYKQPLDHFNYQPQSYV 68
           + L  LLL  ++     +  LG    +   TP        +  Y++Q +DHF +      
Sbjct: 7   LLLSFLLLGAATTIPPRLKTLGSPHLSASPTPDPAVARKYSVLYFEQKVDHFGFADMR-- 66

Query: 69  TFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKAMSVYLEHRF 128
           TF QRY++  K+W+       I  + G E DI       GF    A + KAM V+ EHR+
Sbjct: 67  TFKQRYLVADKHWQ--RNGGSILFYTGNEGDIVWFCNNTGFMWDVAEELKAMLVFAEHRY 126

Query: 129 YGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMF-AYDTSPIIVMGASYGG 186
           YG+S+PFG  + + K+        S QALAD+AEL+ H++K        P+I +G SYGG
Sbjct: 127 YGESLPFG--QDSFKDSQHLNFLTSEQALADFAELIRHLEKTIPGAQGQPVIAIGGSYGG 180

[Close]

BLAST of Chy12G213450 vs. ExPASy TrEMBL
Match: A0A1S3CL25 (lysosomal Pro-X carboxypeptidase-like OS=Cucumis melo OX=3656 GN=LOC103502181 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.1e-92
Identity = 169/185 (91.35%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MDCIVSQSIFLPLLLLFISSCARGHIPVLGVQRRAFQSTPQQLDGLATFYYKQPLDHFNY 60
           MD IVS+SIFLPLLLL ISSC  GHIP+LG+QRR F S PQQLDGLATFYYKQPLDHFNY
Sbjct: 1   MDYIVSKSIFLPLLLLSISSCVCGHIPILGMQRRPFHSRPQQLDGLATFYYKQPLDHFNY 60

Query: 61  QPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKAMSV 120
           QPQSYVTFDQRYIIDFK+W+GINPKTPIFAFLGAESDIDNDVPYVGFPLRFAS+YKAMSV
Sbjct: 61  QPQSYVTFDQRYIIDFKHWEGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASRYKAMSV 120

Query: 121 YLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPIIVMG 180
           YLEHRFYGKSVPFGS EKAMKNGSIRGH NSAQALADYAELLLHIKK FAYDTSPIIVMG
Sbjct: 121 YLEHRFYGKSVPFGSTEKAMKNGSIRGHLNSAQALADYAELLLHIKKKFAYDTSPIIVMG 180

Query: 181 ASYGG 186
           ASYGG
Sbjct: 181 ASYGG 185

[Close]

BLAST of Chy12G213450 vs. ExPASy TrEMBL
Match: A0A6J1FDJ0 (lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444393 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.6e-74
Identity = 140/188 (74.47%), Postives = 159/188 (84.57%), Query Frame = 0


Query: 1   MDCIVSQSIFLPLLLLFISSCARGHIPVLGVQRRAFQSTPQQL---DGLATFYYKQPLDH 60
           MD I+SQSI LPLLLL +SS A  HIP LGVQRRA Q+ PQQ    DGLATFYYKQPLDH
Sbjct: 1   MDSILSQSIVLPLLLL-VSSYASAHIPRLGVQRRASQNKPQQASTPDGLATFYYKQPLDH 60

Query: 61  FNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKA 120
           FNY PQSY TFDQRY+I+FK+W+G+NP  PI  F GAE DID+D+ ++ FP+RFAS+YKA
Sbjct: 61  FNYLPQSYDTFDQRYVINFKHWQGVNPNAPIIVFFGAEEDIDSDISFIDFPIRFASRYKA 120

Query: 121 MSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPII 180
           M VYLEHRFYGKSVPFGS+EKAMKN S+RGH NSAQALADYAE+LLHIK+MFA  TSPII
Sbjct: 121 MLVYLEHRFYGKSVPFGSIEKAMKNASVRGHLNSAQALADYAEVLLHIKQMFASQTSPII 180

Query: 181 VMGASYGG 186
           V+G SYGG
Sbjct: 181 VIGGSYGG 187

[Close]

BLAST of Chy12G213450 vs. ExPASy TrEMBL
Match: A0A6J1FJ87 (lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444394 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 7.1e-72
Identity = 135/188 (71.81%), Postives = 158/188 (84.04%), Query Frame = 0


Query: 1   MDCIVSQSIFLPLLLLFISSCARGHIPVLGVQRRAFQSTPQQ---LDGLATFYYKQPLDH 60
           MD I+SQSI LPLLLL +SS A  HIP LG+QRRA ++ PQQ   LDGLATFYYKQPLDH
Sbjct: 1   MDSILSQSIVLPLLLLLVSSYASAHIPRLGLQRRASRNKPQQASTLDGLATFYYKQPLDH 60

Query: 61  FNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKA 120
           FNYQPQSY TFDQRY+I+FK+W+G+N   PI    G+E D+++D+ ++ FP+R AS+YKA
Sbjct: 61  FNYQPQSYDTFDQRYVINFKHWQGVNLNAPIIVGFGSEGDVEDDISFLDFPIRLASRYKA 120

Query: 121 MSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPII 180
           M VYLEHRFYGKSVPFGS+EKAMKN S+RG+ NSAQALADYAE+LLHIKKMFA  TSP I
Sbjct: 121 MLVYLEHRFYGKSVPFGSIEKAMKNASVRGYLNSAQALADYAEMLLHIKKMFASQTSPTI 180

Query: 181 VMGASYGG 186
           VMGASYGG
Sbjct: 181 VMGASYGG 188

[Close]

BLAST of Chy12G213450 vs. ExPASy TrEMBL
Match: A0A6J1DJ73 (lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC111020537 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.2e-71
Identity = 134/188 (71.28%), Postives = 158/188 (84.04%), Query Frame = 0


Query: 1   MDCIVSQSIFLPLLLLFISSCARGHIPVLGVQRRAFQSTPQQL---DGLATFYYKQPLDH 60
           MD IVS+SI + LLLL +S     HIP LG  RR FQ+ PQ+L   D LATFYYKQPLDH
Sbjct: 1   MDYIVSRSILVSLLLLLVSPYVSAHIPHLGRLRRPFQNKPQKLGMSDELATFYYKQPLDH 60

Query: 61  FNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKA 120
           FNYQPQS VTFDQRY+IDFKYWKG+NP TPIFAF GAE ++D+D+P +G PL FAS+YKA
Sbjct: 61  FNYQPQSDVTFDQRYVIDFKYWKGVNPSTPIFAFFGAEENLDDDIPSIGLPLNFASRYKA 120

Query: 121 MSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPII 180
           M VYLEHRFYGKS+PFGSL+KAM+N +IRG+ +SAQALADYA++LLH+KKMFA +TSPII
Sbjct: 121 MLVYLEHRFYGKSIPFGSLKKAMENATIRGYLSSAQALADYAQVLLHVKKMFAAETSPII 180

Query: 181 VMGASYGG 186
           V+G SYGG
Sbjct: 181 VIGGSYGG 188

[Close]

BLAST of Chy12G213450 vs. ExPASy TrEMBL
Match: A0A6J1DHH2 (lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC111020528 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 8.1e-60
Identity = 113/156 (72.44%), Postives = 134/156 (85.90%), Query Frame = 0


Query: 33  RRAFQSTPQQL---DGLATFYYKQPLDHFNYQPQSYVTFDQRYIIDFKYWKGINPKTPIF 92
           RR+FQ+ PQ+L   D L TFYYKQ LDHFNYQPQSY+TFDQRYII+FKYW+G+NP  PIF
Sbjct: 56  RRSFQNKPQELDIYDELVTFYYKQSLDHFNYQPQSYITFDQRYIINFKYWEGVNPNIPIF 115

Query: 93  AFLGAESDIDNDVPYVGFPLRFASQYKAMSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHF 152
           A+LGAE  +DND   + F L FAS+YKAM VYLEHRFYG+S+PFGSLEKAMKN +IRGH 
Sbjct: 116 AYLGAEGSLDND---LSFTLPFASRYKAMVVYLEHRFYGQSIPFGSLEKAMKNTTIRGHL 175

Query: 153 NSAQALADYAELLLHIKKMFAYDTSPIIVMGASYGG 186
           +SAQALADYA+++LH+KKMFA +TSPIIV+G SYGG
Sbjct: 176 SSAQALADYAQVILHVKKMFAAETSPIIVIGGSYGG 208

[Close]

BLAST of Chy12G213450 vs. NCBI nr
Match: XP_031739623.1 (lysosomal Pro-X carboxypeptidase [Cucumis sativus] >KAE8649460.1 hypothetical protein Csa_018870 [Cucumis sativus])

HSP 1 Score: 364 bits (934), Expect = 4.65e-122
Identity = 180/186 (96.77%), Postives = 184/186 (98.92%), Query Frame = 0


Query: 1   MDCIVSQSIFLP-LLLLFISSCARGHIPVLGVQRRAFQSTPQQLDGLATFYYKQPLDHFN 60
           MDCIVSQSIFLP LLLLFISSCARGHIPVLGVQRRAFQSTPQQ DGLATFYYKQPLDHFN
Sbjct: 7   MDCIVSQSIFLPPLLLLFISSCARGHIPVLGVQRRAFQSTPQQSDGLATFYYKQPLDHFN 66

Query: 61  YQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKAMS 120
           YQPQSYVTFDQRYIIDFKYW+GINPKTPIFA+LGAESDIDNDVPYVGFPLRFASQYKAMS
Sbjct: 67  YQPQSYVTFDQRYIIDFKYWEGINPKTPIFAYLGAESDIDNDVPYVGFPLRFASQYKAMS 126

Query: 121 VYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPIIVM 180
           VYLEHRFYGKS+PFGSLEKAMKNGSIRG+FNSAQALADYAELLLHIKKMFAYDTSPIIVM
Sbjct: 127 VYLEHRFYGKSIPFGSLEKAMKNGSIRGYFNSAQALADYAELLLHIKKMFAYDTSPIIVM 186

Query: 181 GASYGG 185
           GASYGG
Sbjct: 187 GASYGG 192

[Close]

BLAST of Chy12G213450 vs. NCBI nr
Match: XP_031745605.1 (lysosomal Pro-X carboxypeptidase-like [Cucumis sativus] >KGN64893.2 hypothetical protein Csa_022850 [Cucumis sativus])

HSP 1 Score: 360 bits (925), Expect = 1.07e-120
Identity = 179/186 (96.24%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 1   MDCIVSQSIFLPLLLL-FISSCARGHIPVLGVQRRAFQSTPQQLDGLATFYYKQPLDHFN 60
           MDCIVSQSIFLPLLLL FISSCARGHIPVLGVQRRAFQSTPQQ DGLATFYYKQPLDHFN
Sbjct: 7   MDCIVSQSIFLPLLLLLFISSCARGHIPVLGVQRRAFQSTPQQSDGLATFYYKQPLDHFN 66

Query: 61  YQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKAMS 120
           YQPQS VTFDQRYIIDFKYW+GINPKTPIFA+LGAESDIDNDVPYVGFPLRFASQYKAMS
Sbjct: 67  YQPQSSVTFDQRYIIDFKYWEGINPKTPIFAYLGAESDIDNDVPYVGFPLRFASQYKAMS 126

Query: 121 VYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPIIVM 180
           VYLEHRFYGKS+PFGSLEKAMKNGSIRG+FNSAQALADYAELLLHIKKMFAYDTSPIIVM
Sbjct: 127 VYLEHRFYGKSIPFGSLEKAMKNGSIRGYFNSAQALADYAELLLHIKKMFAYDTSPIIVM 186

Query: 181 GASYGG 185
           GASYGG
Sbjct: 187 GASYGG 192

[Close]

BLAST of Chy12G213450 vs. NCBI nr
Match: XP_008464256.1 (PREDICTED: lysosomal Pro-X carboxypeptidase-like [Cucumis melo])

HSP 1 Score: 343 bits (881), Expect = 8.35e-116
Identity = 169/185 (91.35%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MDCIVSQSIFLPLLLLFISSCARGHIPVLGVQRRAFQSTPQQLDGLATFYYKQPLDHFNY 60
           MD IVS+SIFLPLLLL ISSC  GHIP+LG+QRR F S PQQLDGLATFYYKQPLDHFNY
Sbjct: 1   MDYIVSKSIFLPLLLLSISSCVCGHIPILGMQRRPFHSRPQQLDGLATFYYKQPLDHFNY 60

Query: 61  QPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKAMSV 120
           QPQSYVTFDQRYIIDFK+W+GINPKTPIFAFLGAESDIDNDVPYVGFPLRFAS+YKAMSV
Sbjct: 61  QPQSYVTFDQRYIIDFKHWEGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASRYKAMSV 120

Query: 121 YLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPIIVMG 180
           YLEHRFYGKSVPFGS EKAMKNGSIRGH NSAQALADYAELLLHIKK FAYDTSPIIVMG
Sbjct: 121 YLEHRFYGKSVPFGSTEKAMKNGSIRGHLNSAQALADYAELLLHIKKKFAYDTSPIIVMG 180

Query: 181 ASYGG 185
           ASYGG
Sbjct: 181 ASYGG 185

[Close]

BLAST of Chy12G213450 vs. NCBI nr
Match: XP_023538113.1 (lysosomal Pro-X carboxypeptidase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 290 bits (742), Expect = 3.36e-93
Identity = 143/188 (76.06%), Postives = 160/188 (85.11%), Query Frame = 0


Query: 1   MDCIVSQSIFLPLLLLFISSCARGHIPVLGVQRRAFQSTPQQ---LDGLATFYYKQPLDH 60
           MD I+SQSI LPLLLL ISS A  HIP LGVQRRA ++ PQQ   LDGLATFYYKQPLDH
Sbjct: 1   MDSILSQSIVLPLLLLLISSYASAHIPRLGVQRRASRNKPQQASTLDGLATFYYKQPLDH 60

Query: 61  FNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKA 120
           FNYQPQSY TFDQRY+IDFKYW+G+NP  PI  F GAE DID+D+ ++ FP+RFAS+YKA
Sbjct: 61  FNYQPQSYDTFDQRYVIDFKYWQGVNPNAPIVVFFGAEEDIDDDISFIDFPIRFASRYKA 120

Query: 121 MSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPII 180
           M VYLEHRFYGKSVPFGS+EKAMKN S+RG+ NSAQALADYAE+LLHIKKM A  TSPII
Sbjct: 121 MLVYLEHRFYGKSVPFGSIEKAMKNASVRGYLNSAQALADYAEVLLHIKKMLASQTSPII 180

Query: 181 VMGASYGG 185
           V+G SYGG
Sbjct: 181 VIGGSYGG 188

[Close]

BLAST of Chy12G213450 vs. NCBI nr
Match: XP_023538112.1 (lysosomal Pro-X carboxypeptidase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 290 bits (742), Expect = 3.87e-93
Identity = 143/188 (76.06%), Postives = 160/188 (85.11%), Query Frame = 0


Query: 1   MDCIVSQSIFLPLLLLFISSCARGHIPVLGVQRRAFQSTPQQ---LDGLATFYYKQPLDH 60
           MD I+SQSI LPLLLL ISS A  HIP LGVQRRA ++ PQQ   LDGLATFYYKQPLDH
Sbjct: 1   MDSILSQSIVLPLLLLLISSYASAHIPRLGVQRRASRNKPQQASTLDGLATFYYKQPLDH 60

Query: 61  FNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKA 120
           FNYQPQSY TFDQRY+IDFKYW+G+NP  PI  F GAE DID+D+ ++ FP+RFAS+YKA
Sbjct: 61  FNYQPQSYDTFDQRYVIDFKYWQGVNPNAPIVVFFGAEEDIDDDISFIDFPIRFASRYKA 120

Query: 121 MSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPII 180
           M VYLEHRFYGKSVPFGS+EKAMKN S+RG+ NSAQALADYAE+LLHIKKM A  TSPII
Sbjct: 121 MLVYLEHRFYGKSVPFGSIEKAMKNASVRGYLNSAQALADYAEVLLHIKKMLASQTSPII 180

Query: 181 VMGASYGG 185
           V+G SYGG
Sbjct: 181 VIGGSYGG 188

[Close]

BLAST of Chy12G213450 vs. TAIR 10
Match: AT5G22860.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 166.0 bits (419), Expect = 2.9e-41
Identity = 84/187 (44.92%), Postives = 120/187 (64.17%), Query Frame = 0


Query: 13  LLLLFISS--------CARGHIPVLGVQRRAFQSTP----QQLD--GLATFYYKQPLDHF 72
           +L+LFI S         A   I  LG+  +  ++ P    Q++D   L  +Y+ Q LDHF
Sbjct: 7   ILILFIFSTSSSYLIPLAHSKIARLGISSKTLKNEPDGSTQKVDESNLKMYYFNQTLDHF 66

Query: 73  NYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKAM 132
            + P+SY+TF QRY ID  +W G     PI AFLG ES +D+D+  +GF      +  A+
Sbjct: 67  TFTPESYMTFQQRYAIDSTHWGGAKANAPILAFLGEESSLDSDLAAIGFLRDNGPRLNAL 126

Query: 133 SVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPIIV 186
            VY+EHR+YG+++PFGS E+A+KN S  G+ N+AQALADYA +LLH+K+ ++ + SPIIV
Sbjct: 127 LVYIEHRYYGETMPFGSAEEALKNASTLGYLNAAQALADYAAILLHVKEKYSTNHSPIIV 186

[Close]

BLAST of Chy12G213450 vs. TAIR 10
Match: AT5G22860.2 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 166.0 bits (419), Expect = 2.9e-41
Identity = 84/187 (44.92%), Postives = 120/187 (64.17%), Query Frame = 0


Query: 13  LLLLFISS--------CARGHIPVLGVQRRAFQSTP----QQLD--GLATFYYKQPLDHF 72
           +L+LFI S         A   I  LG+  +  ++ P    Q++D   L  +Y+ Q LDHF
Sbjct: 7   ILILFIFSTSSSYLIPLAHSKIARLGISSKTLKNEPDGSTQKVDESNLKMYYFNQTLDHF 66

Query: 73  NYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKAM 132
            + P+SY+TF QRY ID  +W G     PI AFLG ES +D+D+  +GF      +  A+
Sbjct: 67  TFTPESYMTFQQRYAIDSTHWGGAKANAPILAFLGEESSLDSDLAAIGFLRDNGPRLNAL 126

Query: 133 SVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPIIV 186
            VY+EHR+YG+++PFGS E+A+KN S  G+ N+AQALADYA +LLH+K+ ++ + SPIIV
Sbjct: 127 LVYIEHRYYGETMPFGSAEEALKNASTLGYLNAAQALADYAAILLHVKEKYSTNHSPIIV 186

[Close]

BLAST of Chy12G213450 vs. TAIR 10
Match: AT2G24280.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 5.6e-29
Identity = 71/186 (38.17%), Postives = 107/186 (57.53%), Query Frame = 0


Query: 8   SIFLPLLLLFISSCARG--------HIPVLGVQRRAFQSTPQQLDGLATFYYKQPLDHFN 67
           S  L L+ LF S  A          H+  L ++++  +S  +      T Y+ Q LDHF+
Sbjct: 2   SACLCLVFLFFSIVAEATYSPGGFHHLSSLRLKKKVSKSKHEL--PFETRYFPQNLDHFS 61

Query: 68  YQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGFPLRFASQYKAMS 127
           + P SY  F Q+Y+I+ ++W+      PIF + G E DID      GF L  A +++A+ 
Sbjct: 62  FTPDSYKVFHQKYLINNRFWR---KGGPIFVYTGNEGDIDWFASNTGFMLDIAPKFRALL 121

Query: 128 VYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKKMFAYDTSPIIVM 186
           V++EHRFYG+S PFG  +K+ K+    G+ NS QALADYA L+  +K+  + + SP++V 
Sbjct: 122 VFIEHRFYGESTPFG--KKSHKSAETLGYLNSQQALADYAILIRSLKQNLSSEASPVVVF 180

[Close]

BLAST of Chy12G213450 vs. TAIR 10
Match: AT5G65760.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 105.9 bits (263), Expect = 3.5e-23
Identity = 51/138 (36.96%), Postives = 82/138 (59.42%), Query Frame = 0


Query: 48  TFYYKQPLDHFNYQPQSYVTFDQRYIIDFKYWKGINPKTPIFAFLGAESDIDNDVPYVGF 107
           T ++ Q LDHF++       F QRY+I+  +W G +   PIF + G E DI+      GF
Sbjct: 60  TKFFSQQLDHFSF--ADLPKFSQRYLINSDHWLGASALGPIFLYCGNEGDIEWFATNSGF 119

Query: 108 PLRFASQYKAMSVYLEHRFYGKSVPFGSLEKAMKNGSIRGHFNSAQALADYAELLLHIKK 167
               A ++ A+ V+ EHR+YG+S+P+GS E+A KN +   +  + QALAD+A  +  +K+
Sbjct: 120 IWDIAPKFGALLVFPEHRYYGESMPYGSREEAYKNATTLSYLTTEQALADFAVFVTDLKR 179

Query: 168 MFAYDTSPIIVMGASYGG 186
             + +  P+++ G SYGG
Sbjct: 180 NLSAEACPVVLFGGSYGG 195

[Close]

BLAST of Chy12G213450 vs. TAIR 10
Match: AT4G36195.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.5e-07
Identity = 48/142 (33.80%), Postives = 67/142 (47.18%), Query Frame = 0


Query: 50  YYKQPLDHFNYQPQSYVTFDQRYIIDFKYWKGIN-PKTPIFAFLGAE---SDIDNDVPYV 109
           ++ Q LDH  Y P  +  F QRY   ++Y   +  P  PIF  +  E   + I ND   V
Sbjct: 49  WFNQTLDH--YSPSDHREFKQRY---YEYLDHLRVPDGPIFMMICGEGPCNGIPNDYITV 108

Query: 110 GFPLRFASQYKAMSVYLEHRFYGKSVPFGSL-EKAMKNGSIRGHFNSAQALADYAELLLH 169
                 A ++ A  V LEHR+YGKS PF SL  + +K  S +       A   Y +  L+
Sbjct: 109 -----LAKKFDAGIVSLEHRYYGKSSPFKSLATENLKYLSSKQALFDLAAFRQYYQDSLN 168

Query: 170 IKKMFAYDT-SPIIVMGASYGG 186
           +K   + D  +P    GASY G
Sbjct: 169 VKFNRSGDVENPWFFFGASYSG 180

The following BLAST results are available for this feature:

BLAST of Chy12G213450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42785	1.0e-19	37.58	Lysosomal Pro-X carboxypeptidase OS=Homo sapiens OX=9606 GN=PRCP PE=1 SV=1	[more]
Q5RBU7	1.3e-19	37.58	Lysosomal Pro-X carboxypeptidase OS=Pongo abelii OX=9601 GN=PRCP PE=2 SV=1	[more]
Q9EPB1	5.1e-19	38.85	Dipeptidyl peptidase 2 OS=Rattus norvegicus OX=10116 GN=Dpp7 PE=1 SV=1	[more]
Q9ET22	8.7e-19	38.13	Dipeptidyl peptidase 2 OS=Mus musculus OX=10090 GN=Dpp7 PE=1 SV=2	[more]
Q7TMR0	1.3e-17	33.33	Lysosomal Pro-X carboxypeptidase OS=Mus musculus OX=10090 GN=Prcp PE=1 SV=2	[more]

BLAST of Chy12G213450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CL25	2.1e-92	91.35	lysosomal Pro-X carboxypeptidase-like OS=Cucumis melo OX=3656 GN=LOC103502181 PE...	[more]
A0A6J1FDJ0	7.6e-74	74.47	lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444...	[more]
A0A6J1FJ87	7.1e-72	71.81	lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444...	[more]
A0A6J1DJ73	1.2e-71	71.28	lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC11102...	[more]
A0A6J1DHH2	8.1e-60	72.44	lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC11102...	[more]

BLAST of Chy12G213450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739623.1	4.65e-122	96.77	lysosomal Pro-X carboxypeptidase [Cucumis sativus] >KAE8649460.1 hypothetical pr...	[more]
XP_031745605.1	1.07e-120	96.24	lysosomal Pro-X carboxypeptidase-like [Cucumis sativus] >KGN64893.2 hypothetical...	[more]
XP_008464256.1	8.35e-116	91.35	PREDICTED: lysosomal Pro-X carboxypeptidase-like [Cucumis melo]	[more]
XP_023538113.1	3.36e-93	76.06	lysosomal Pro-X carboxypeptidase-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023538112.1	3.87e-93	76.06	lysosomal Pro-X carboxypeptidase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy12G213450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G22860.1	2.9e-41	44.92	Serine carboxypeptidase S28 family protein	[more]
AT5G22860.2	2.9e-41	44.92	Serine carboxypeptidase S28 family protein	[more]
AT2G24280.1	5.6e-29	38.17	alpha/beta-Hydrolases superfamily protein	[more]
AT5G65760.1	3.5e-23	36.96	Serine carboxypeptidase S28 family protein	[more]
AT4G36195.1	3.5e-07	33.80	Serine carboxypeptidase S28 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008758	Peptidase S28	PFAM	PF05577	Peptidase_S28	coord: 53..185 e-value: 4.3E-25 score: 88.4
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 44..185 e-value: 4.8E-41 score: 142.8
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 50..185
None	No IPR available	PANTHER	PTHR11010	PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED	coord: 22..185
None	No IPR available	PANTHER	PTHR11010:SF96	PROLYLCARBOXYPEPTIDASE-LIKE PROTEIN-RELATED	coord: 22..185

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G213450.1	Chy12G213450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004180	carboxypeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity