Chy12G212470 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G212470
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	subtilisin-like protease SBT5.4
Location	chrH12: 9263042 .. 9267445 (+)
RNA-Seq Expression	Chy12G212470
Synteny	Chy12G212470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCATCGGAAGATGAAGATCTATAATTCCATTCTCATTTTATTTTCACTCCTTTTGATAATATCACCTGCCATGGCTGCCAAAAAGGTAATCAAAATATTAGAAAACAGATTATTCTTAAACAAGTCTTGAAGTTGTTTTATTATGCATGCATTATCTAATATCTACACCCATTTTGTTCTTTATTATTAAAAAAGATATATTGATTTGGTTTGGTTCATTAGATTGCTTTTTGGGTTGTGATTTTTTTCTTCACAAAGCCCTAAAAATTAAAATTGTTGTTTTCCTGATCTTTATTATTTGTTTTTTTTGTATACACTGATTGATTCAGTCTATACTTTCTATGTTCATAAACATATTATTAAACAAACCCCAAAACTAATTGCTTTTATTATTATTATTATTATTGATTTGGTTGGGATAAATGAGGTGTTTTGGGTTGTGATTCTTTTGTTTTGTACAAAAAATTCCTAAATTTTTGTTGTTTTCTTCTCTATTACTATTATTCTTTAGTCATTTTACGTCTTGATTGACTTATTCGTATAATATATATGTTTCTAAACATATTATTTTATAAACCGTAGACAAATTTCCTTTTCTTAATATATATATATAATTATCGATTACGTTTGGTCAATATGAAGTGCTATTGGGTTTTGAATTTTTTTTCTTCAGGTTTACAAAACCTTTTTTCTTTTTTTGGTTCTTGAACTCAGTTGATTGGGTGGTGTACTTAATTTGTATGTTTCTATGAATTATTAAAAATAATTTTAAGATTAATTGAACATTTTCAAATATAGCAAAAACAATTAGCTCTATTTTGTAAAATAGAGCAAAATCTAATAAATTCTTTAGGTTCAGTTAAGTTTTTTTTTTTTTTTTTGCATAATATAGTTTCATTTTTTTTTAATTTGCTATCGATAAGAGTTTCTCCAAAACTAAATGATTTTTTTTTAATGTTTGGATTTCAGTCTTATATTGTGTTATTGGGATCCCATTCACATGGATTAGAAGTTACAGATGAAGATCTTAAAAGGGTGGCTGATTCCCATCACAAGTTGCTTGGATCCATCTTTGGAAGGTTTGAAATTTTATCTATAATTTCCTCATCATAACAACTTATTTTTCATTTGATTATTTTTTTATAAAAAAAATCTTGTTGTTGTTATTATTATAATTCTTATTTATTAAATTTTAATTATTTTAAAATTGAAAATTTCAGTGATGAGAAGGCAAGAGATGCTATATTTTACTCTTATAAGAAGAATATAAATGGTTTTGCAGCAATTATGGATGAGGAAGAGGCAACCCAGCTTGCAAGTAAAAAAATCAACTTATATACTTTTTTTTTTAAAAAAAAAAATCTATTTTCACATCTTTCAGCATAACCAAGGTTTAATTTAAAAAAAAGAATTATAATAATAGAAACTAAATTGGAAAATTATAAAAATGTGACAGAACACCCGGAAGTAGCAGCAGTATTGCCAAACAGAGCAAAAAAGTTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCCCTTCATCTGCTTGGCGTAGGGCTAAATCTGGAAAAGACGTCATTATCGCCAATCTTGACACTGGTTCACTTCTTTTTTTCTATTTAATTAATAGCAAAAAATTTATTCTTAGTATTTTACAAATTTAGTAGTGAATAATTAAGTAGCATAAATTATTGGAACAAATATAAAAACATAGGATAAAGACTTTGATGGAAGTAAAGTTTAGTTTATGGTCCCAATTAAAATAAATATATATATTTGTTATCTTTTCTGTTTTGGGAAAAGGTGTGTGGCCGGGGTCCAAGAGTTTTGGAGAACATGGGATCGTTGGACCTGTTCCATCAAAGTGGAAAGGAGGTTGCACTGATAAAACCCCTGATAGAGTGCCATGTAACAGGTTTGATCTTTTTGACACTAAAAGTTAATTAACTACTTTGATATTGATTTAAGGTTGATTAATTTTCTTTAATATTATATTGATTTTAAAATAAATAAATATAGGAAACTAATAGGAGCAAAGTACTTCAACAAGGGCTTTCTCGCATACTTGAAGTCTGAGGATTTGACAGCATCGGTGATTAACTCCACTCGCGACTACGACGGTCATGGTTCACACACCTTGTCCACGGCTGGGGGCAGCTATGTGTCCGGCGCCAGTGCGTTCGGGTTAGGCGTTGGAACTGCCAAAGGGGGCTCTCCCAAGGCTCGTGTTGCTGCCTATAAGGTTTGCTGGCCACTTGAGGATGGAGGTTGTTTCGACGCTGACATTGCCGAGGCATTTGACCATGCCATTCACGACCGCGTTGATGTTCTTTCGCTCTCCCTTGGTGGCGAGCCAGCCGATTATTATGATGATGGAATCGCCATTGCAGCCTTCCATGCAATGAAGAAGGGAATCCCTGTGGTGTGCTCGGCTGGTAACTCTGGCCCTGGTGCTCAGACTGTTTCTAATACTGCACCTTGGATTTTGACTGTTGGTGCAAGTACTGTGGATCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGTCACCGTTATATGGTTCGTCTCTTTCACTCTCGAATTGAATGTGTATGAGCTCTATTTATATTTTGTTGGTATTCTATTCATTAGGGTTCGAGCCTTTCAAAAGGATTAAAGGGAGACAAGCTTTATCAATTAATAACTGGAGCTGAAGCGAAAGCGAAGAACGCCACCGCGGAGGTGGCCATGCTTTGCAAACCAAAAACATTGGATCATTCAAAAGTGAAAGGGAAGATATTGGTTTGCTTGAGAGGAGACACTGCAAGAGTAGACAAAGGAGAACAAGCGGCCCTTGCCGGTGCTGTCGGAATGATTCTATGCAATGATGAGCTAAGTGGGTTCGAAACCATCGCAGATCCCCATGTTCTTCCTGCTTCCCATATCAATTACAATGATGGCCAAGCTATTTTCTCTTACATCAAATCCACCAAGTAGTTAATAATAACAAATTTTTTCCCCTTTTCTAAAAGGAAAAAAAAGTTGATTATGATGATATTTGGTTGTTTCTTTGGCAGGAATCCGATGGGGTATTTAATCCCACCATCGGCAAAAGTTAACACCAAACCTGCTCCCACTATGGCAGCCTTTTCATCTCGAGGACCCAACCTTATTTCCCCTGAGATTATCAAGGTTTGCTTTTTCTCAAACCCTTATTATGATTTTCTCTTAAAAATCTATTTTATACTTTAATGGATGTTAAGAACAGTATTAGTCCTCAGAATATTTTAATTAATATTAAAATATATATGTTAATATAATAAATTTAAGCTGATATGCTTTCGTTTTTGAGTTTTGAAAACAGTTTAATATGATTGCAACATAAACAGTTCACAATATGACGAATCTTTTTAATGTTAAGCAGACATAGCTAACTCTTTCCCACTTAATATATAGCTCAATAACATTAAAGTATATTCCTTCAACGAATTTGTTTGATTTTAAATTTCTCATCTCCCCAAATGGTACTCAAAGAACTCACTCTAAAGTTATGACATATTTTAATTAAATGAATAAATGATAATTTATAGAATCTCACATCTCTCCGAATTAACGACTACTTGAATTAAAATTTCCAGCCAGATGTGACTGCACCGGGTGTGAATATAATTGCAGCATTCTCAGAGGCCGTAAGCCCAACAGGCGAACCATTTGACAATAGAACAGTTCCATTCATAACAATGTCAGGCACCTCCATGTCTTGCCCCCATGTCTCTGGTCTTGTCGGCCTTCTTAGAACTCTCCACCCTCGCTGGAGCCCCTCCGCCATCAAATCTGCCATCATGACCTCTGCCAGAATTCGTGACAACACAAAAAAACCCATGCTCGATGGTGGCTCCCCTGACCTCGCCCCTGCCACCCCCTTTGCCTATGGCTCTGGCCACATTCGCCCTACTGGAGCCATTGACCCCGGCCTCGTCTATGATCTCTCCCCCAATGACTACTTGGAATTCCTCTGCGCCAGTGGCTATAACGAGAAGACCATTCAAGCATTCTCTGATGGCCCTTTCAAATGTCCAGCTTCTGCTTCTATTTTAAACTTCAATTATCCTTCCATTGGGGTTCAGAATCTGACTGGCAGTGTCACCGTCACTAGGAAATTGAAGAACGTTAGCACTCCTGGGGTATACAAAGCCAGAGTTAGGCACCCCAATGGAGTCAAGGTTTTAGTGAAGCCCAAAGTTCTAAAGTTCGAGAGAGTTGGAGAAGAGAAAAGCTTTGAATTGACAATAACTGGAGATGTGCCAGAGGATCAGGTTGTTGACGGCGTGCTGATTTGGACGGATGGCAAGCACTTTGTTAGGAGTCCCATTGTCGTTTCCTCTAGCTTGTTCTGA

mRNA sequence

ATGAAGCATCGGAAGATGAAGATCTATAATTCCATTCTCATTTTATTTTCACTCCTTTTGATAATATCACCTGCCATGGCTGCCAAAAAGAATATAAATGGTTTTGCAGCAATTATGGATGAGGAAGAGGCAACCCAGCTTGCAAAAACTAAATTGGAAAATTATAAAAATGTGACAGAACACCCGGAAGTAGCAGCAGTATTGCCAAACAGAGCAAAAAAGTTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCCCTTCATCTGCTTGGCGTAGGGCTAAATCTGGAAAAGACGTCATTATCGCCAATCTTGACACTGGTGTGTGGCCGGGGTCCAAGAGTTTTGGAGAACATGGGATCGTTGGACCTGTTCCATCAAAGTGGAAAGGAGGTTGCACTGATAAAACCCCTGATAGAGTGCCATGTAACAGGAAACTAATAGGAGCAAAGTACTTCAACAAGGGCTTTCTCGCATACTTGAAGTCTGAGGATTTGACAGCATCGGTGATTAACTCCACTCGCGACTACGACGGTCATGGTTCACACACCTTGTCCACGGCTGGGGGCAGCTATGTGTCCGGCGCCAGTGCGTTCGGGTTAGGCGTTGGAACTGCCAAAGGGGGCTCTCCCAAGGCTCGTGTTGCTGCCTATAAGGTTTGCTGGCCACTTGAGGATGGAGGTTGTTTCGACGCTGACATTGCCGAGGCATTTGACCATGCCATTCACGACCGCGTTGATGTTCTTTCGCTCTCCCTTGGTGGCGAGCCAGCCGATTATTATGATGATGGAATCGCCATTGCAGCCTTCCATGCAATGAAGAAGGGAATCCCTGTGGTGTGCTCGGCTGGTAACTCTGGCCCTGGTGCTCAGACTGTTTCTAATACTGCACCTTGGATTTTGACTGTTGGTGCAAGTACTGTGGATCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGTCACCGTTATATGGGTTCGAGCCTTTCAAAAGGATTAAAGGGAGACAAGCTTTATCAATTAATAACTGGAGCTGAAGCGAAAGCGAAGAACGCCACCGCGGAGGTGGCCATGCTTTGCAAACCAAAAACATTGGATCATTCAAAAGTGAAAGGGAAGATATTGGTTTGCTTGAGAGGAGACACTGCAAGAGTAGACAAAGGAGAACAAGCGGCCCTTGCCGGTGCTGTCGGAATGATTCTATGCAATGATGAGCTAAGTGGGTTCGAAACCATCGCAGATCCCCATGTTCTTCCTGCTTCCCATATCAATTACAATGATGGCCAAGCTATTTTCTCTTACATCAAATCCACCAAGAATCCGATGGGGTATTTAATCCCACCATCGGCAAAAGTTAACACCAAACCTGCTCCCACTATGGCAGCCTTTTCATCTCGAGGACCCAACCTTATTTCCCCTGAGATTATCAAGCCAGATGTGACTGCACCGGGTGTGAATATAATTGCAGCATTCTCAGAGGCCGTAAGCCCAACAGGCGAACCATTTGACAATAGAACAGTTCCATTCATAACAATGTCAGGCACCTCCATGTCTTGCCCCCATGTCTCTGGTCTTGTCGGCCTTCTTAGAACTCTCCACCCTCGCTGGAGCCCCTCCGCCATCAAATCTGCCATCATGACCTCTGCCAGAATTCGTGACAACACAAAAAAACCCATGCTCGATGGTGGCTCCCCTGACCTCGCCCCTGCCACCCCCTTTGCCTATGGCTCTGGCCACATTCGCCCTACTGGAGCCATTGACCCCGGCCTCGTCTATGATCTCTCCCCCAATGACTACTTGGAATTCCTCTGCGCCAGTGGCTATAACGAGAAGACCATTCAAGCATTCTCTGATGGCCCTTTCAAATGTCCAGCTTCTGCTTCTATTTTAAACTTCAATTATCCTTCCATTGGGGTTCAGAATCTGACTGGCAGTGTCACCGTCACTAGGAAATTGAAGAACGTTAGCACTCCTGGGGTATACAAAGCCAGAGTTAGGCACCCCAATGGAGTCAAGGTTTTAGTGAAGCCCAAAGTTCTAAAGTTCGAGAGAGTTGGAGAAGAGAAAAGCTTTGAATTGACAATAACTGGAGATGTGCCAGAGGATCAGGTTGTTGACGGCGTGCTGATTTGGACGGATGGCAAGCACTTTGTTAGGAGTCCCATTGTCGTTTCCTCTAGCTTGTTCTGA

Coding sequence (CDS)

ATGAAGCATCGGAAGATGAAGATCTATAATTCCATTCTCATTTTATTTTCACTCCTTTTGATAATATCACCTGCCATGGCTGCCAAAAAGAATATAAATGGTTTTGCAGCAATTATGGATGAGGAAGAGGCAACCCAGCTTGCAAAAACTAAATTGGAAAATTATAAAAATGTGACAGAACACCCGGAAGTAGCAGCAGTATTGCCAAACAGAGCAAAAAAGTTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCCCTTCATCTGCTTGGCGTAGGGCTAAATCTGGAAAAGACGTCATTATCGCCAATCTTGACACTGGTGTGTGGCCGGGGTCCAAGAGTTTTGGAGAACATGGGATCGTTGGACCTGTTCCATCAAAGTGGAAAGGAGGTTGCACTGATAAAACCCCTGATAGAGTGCCATGTAACAGGAAACTAATAGGAGCAAAGTACTTCAACAAGGGCTTTCTCGCATACTTGAAGTCTGAGGATTTGACAGCATCGGTGATTAACTCCACTCGCGACTACGACGGTCATGGTTCACACACCTTGTCCACGGCTGGGGGCAGCTATGTGTCCGGCGCCAGTGCGTTCGGGTTAGGCGTTGGAACTGCCAAAGGGGGCTCTCCCAAGGCTCGTGTTGCTGCCTATAAGGTTTGCTGGCCACTTGAGGATGGAGGTTGTTTCGACGCTGACATTGCCGAGGCATTTGACCATGCCATTCACGACCGCGTTGATGTTCTTTCGCTCTCCCTTGGTGGCGAGCCAGCCGATTATTATGATGATGGAATCGCCATTGCAGCCTTCCATGCAATGAAGAAGGGAATCCCTGTGGTGTGCTCGGCTGGTAACTCTGGCCCTGGTGCTCAGACTGTTTCTAATACTGCACCTTGGATTTTGACTGTTGGTGCAAGTACTGTGGATCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGTCACCGTTATATGGGTTCGAGCCTTTCAAAAGGATTAAAGGGAGACAAGCTTTATCAATTAATAACTGGAGCTGAAGCGAAAGCGAAGAACGCCACCGCGGAGGTGGCCATGCTTTGCAAACCAAAAACATTGGATCATTCAAAAGTGAAAGGGAAGATATTGGTTTGCTTGAGAGGAGACACTGCAAGAGTAGACAAAGGAGAACAAGCGGCCCTTGCCGGTGCTGTCGGAATGATTCTATGCAATGATGAGCTAAGTGGGTTCGAAACCATCGCAGATCCCCATGTTCTTCCTGCTTCCCATATCAATTACAATGATGGCCAAGCTATTTTCTCTTACATCAAATCCACCAAGAATCCGATGGGGTATTTAATCCCACCATCGGCAAAAGTTAACACCAAACCTGCTCCCACTATGGCAGCCTTTTCATCTCGAGGACCCAACCTTATTTCCCCTGAGATTATCAAGCCAGATGTGACTGCACCGGGTGTGAATATAATTGCAGCATTCTCAGAGGCCGTAAGCCCAACAGGCGAACCATTTGACAATAGAACAGTTCCATTCATAACAATGTCAGGCACCTCCATGTCTTGCCCCCATGTCTCTGGTCTTGTCGGCCTTCTTAGAACTCTCCACCCTCGCTGGAGCCCCTCCGCCATCAAATCTGCCATCATGACCTCTGCCAGAATTCGTGACAACACAAAAAAACCCATGCTCGATGGTGGCTCCCCTGACCTCGCCCCTGCCACCCCCTTTGCCTATGGCTCTGGCCACATTCGCCCTACTGGAGCCATTGACCCCGGCCTCGTCTATGATCTCTCCCCCAATGACTACTTGGAATTCCTCTGCGCCAGTGGCTATAACGAGAAGACCATTCAAGCATTCTCTGATGGCCCTTTCAAATGTCCAGCTTCTGCTTCTATTTTAAACTTCAATTATCCTTCCATTGGGGTTCAGAATCTGACTGGCAGTGTCACCGTCACTAGGAAATTGAAGAACGTTAGCACTCCTGGGGTATACAAAGCCAGAGTTAGGCACCCCAATGGAGTCAAGGTTTTAGTGAAGCCCAAAGTTCTAAAGTTCGAGAGAGTTGGAGAAGAGAAAAGCTTTGAATTGACAATAACTGGAGATGTGCCAGAGGATCAGGTTGTTGACGGCGTGCTGATTTGGACGGATGGCAAGCACTTTGTTAGGAGTCCCATTGTCGTTTCCTCTAGCTTGTTCTGA

Protein sequence

MKHRKMKIYNSILILFSLLLIISPAMAAKKNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRVPCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNGHRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKARVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF*

Homology

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BLAST of Chy12G212470 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 7.7e-244
Identity = 429/709 (60.51%), Postives = 520/709 (73.34%), Query Frame = 0


Query: 29  KKNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           K++INGFAAI+DE EA ++AK           HP+V +V PN+ +KLHTTHSW FM L K
Sbjct: 90  KRHINGFAAILDENEAAEIAK-----------HPDVVSVFPNKGRKLHTTHSWNFMLLAK 149

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRV 148
           NGV+  SS W +A  G+D IIANLDTGVWP SKSF + G  G VP++WKG C       V
Sbjct: 150 NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD----V 209

Query: 149 PCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGL 208
           PCNRKLIGA+YFNKG+LAY      + +   + RD+DGHGSHTLSTA G++V GA+ FG+
Sbjct: 210 PCNRKLIGARYFNKGYLAYTGLP--SNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGI 269

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAEAFDHAIHDRVDVLSLSLGGEPADY 268
           G GTA GGSPKARVAAYKVCWP  DG  CFDADI  A + AI D VDVLS S+GG+  DY
Sbjct: 270 GNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDY 329

Query: 269 YDDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQN 328
             DGIAI +FHA+K G+ VVCSAGNSGP + TVSN APW++TVGAS++DREFQA VEL+N
Sbjct: 330 MSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKN 389

Query: 329 GHRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRG 388
           G  + G+SLSK L  +K+Y LI+ A+A   N     A+LCK  +LD  KVKGKILVCLRG
Sbjct: 390 GQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRG 449

Query: 389 DTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKN 448
           D ARVDKG QAA AGA GM+LCND+ SG E I+D HVLPAS I+Y DG+ +FSY+ STK+
Sbjct: 450 DNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKD 509

Query: 449 PMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGE 508
           P GY+  P+A +NTKPAP MA+FSSRGPN I+P I+KPD+TAPGVNIIAAF+EA  PT  
Sbjct: 510 PKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDL 569

Query: 509 PFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPML 568
             DNR  PF T SGTSMSCPH+SG+VGLL+TLHP WSP+AI+SAIMT++R R+N +KPM+
Sbjct: 570 DSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMV 629

Query: 569 DGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGP 628
           D        A PF+YGSGH++P  A  PGLVYDL+  DYL+FLCA GYN   +Q F++ P
Sbjct: 630 D---ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDP 689

Query: 629 -FKCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKARVRHPNGVKVLVKPKV 688
            + C   A++L+FNYPSI V NLTGS+TVTRKLKNV  P  Y AR R P GV+V V+PK 
Sbjct: 690 QYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQ 749

Query: 689 LKFERVGEEKSFELTITG-DVPEDQVVDGVLIWTDGKHFVRSPIVVSSS 735
           L F + GE K F++T+    V     V G L WTD  H+VRSPIVV  S
Sbjct: 750 LTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLS 777

[Close]

BLAST of Chy12G212470 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 6.0e-212
Identity = 382/709 (53.88%), Postives = 492/709 (69.39%), Query Frame = 0


Query: 30  KNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEKN 89
           K+INGFAA +D + A +++K           HPEV +V PN+A KLHTT SW+F+ LE N
Sbjct: 81  KHINGFAAHLDHDLAYEISK-----------HPEVVSVFPNKALKLHTTRSWDFLGLEHN 140

Query: 90  GVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRVP 149
             +P SS WR+A+ G+D IIANLDTGVWP SKSF + G+ GP+PS+WKG C ++      
Sbjct: 141 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATFH 200

Query: 150 CNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGLG 209
           CNRKLIGA+YFNKG+ A +   +   S  +S RD DGHGSHTLSTA G +V G S FG G
Sbjct: 201 CNRKLIGARYFNKGYAAAVGHLN---SSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQG 260

Query: 210 VGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAEAFDHAIHDRVDVLSLSLGGEPADYY 269
            GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHD  DV+S+SLGGEP  ++
Sbjct: 261 NGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFF 320

Query: 270 DDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNG 329
           +D +AI +FHA KK I VVCSAGNSGP   TVSN APW +TVGAST+DREF + + L NG
Sbjct: 321 NDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNG 380

Query: 330 HRYMGSSLSK-GLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRG 389
             Y G SLS   L   K Y ++    AKAKNA+A  A LCK  +LD  K KGKILVCLRG
Sbjct: 381 KHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRG 440

Query: 390 DTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKN 449
              RV+KG   AL G +GM+L N  ++G + +ADPHVLPA+ +   D  A+  YI  TK 
Sbjct: 441 QNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKK 500

Query: 450 PMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGE 509
           P+ ++ P    +  KPAP MA+FSS+GP++++P+I+KPD+TAPGV++IAA++ AVSPT E
Sbjct: 501 PIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNE 560

Query: 510 PFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPML 569
            FD R + F  +SGTSMSCPH+SG+ GLL+T +P WSP+AI+SAIMT+A I D+   P+ 
Sbjct: 561 QFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQ 620

Query: 570 DGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGP 629
           +  +     ATPF++G+GH++P  A++PGLVYDL   DYL FLC+ GYN   I  FS   
Sbjct: 621 NATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNN 680

Query: 630 FKCPA-SASILNFNYPSIGVQNLTGS-VTVTRKLKNVSTPGVYKARVRHPNGVKVLVKPK 689
           F C +   S++N NYPSI V NLT S VTV+R +KNV  P +Y  +V +P GV V VKP 
Sbjct: 681 FTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 740

Query: 690 VLKFERVGEEKSFELTIT---GDVPEDQVVDGVLIWTDGKHFVRSPIVV 732
            L F +VGE+K+F++ +    G+V +  V  G L+W+D KH VRSPIVV
Sbjct: 741 SLNFTKVGEQKTFKVILVKSKGNVAKGYVF-GELVWSDKKHRVRSPIVV 770

[Close]

BLAST of Chy12G212470 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 585.9 bits (1509), Expect = 6.5e-166
Identity = 342/727 (47.04%), Postives = 449/727 (61.76%), Query Frame = 0


Query: 30  KNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEKN 89
           ++INGFAA+++EEEA  +AK           +P V +V  ++  KLHTT SWEF+ L + 
Sbjct: 81  RHINGFAALLEEEEAADIAK-----------NPNVVSVFLSKEHKLHTTRSWEFLGLHRR 140

Query: 90  GVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGC--TDKTPDR 149
           G    +SAW++ + G++ II N+DTGVWP S+SF + G  G VPSKW+GG    +K P  
Sbjct: 141 G---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKG-YGTVPSKWRGGLCQINKLPGS 200

Query: 150 V--PCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASA 209
           +   CNRKLIGA+Y+NK F A+    D    ++++ RD+ GHG+HTLSTAGG++V GA  
Sbjct: 201 MKNTCNRKLIGARYYNKAFEAHNGQLD---PLLHTARDFVGHGTHTLSTAGGNFVPGARV 260

Query: 210 FGLGVGTAKGGSPKARVAAYKVCWPLED-GGCFDADIAEAFDHAIHDRVDVLSLSLGGE- 269
           F +G GTAKGGSP+ARVAAYKVCW L D   C+ AD+  A D AI D VDV+++S G   
Sbjct: 261 FAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSY 320

Query: 270 ---PADYYDDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQ 329
                  + D I+I AFHA+ K I +V SAGN GP   TV+N APW+ T+ AST+DR+F 
Sbjct: 321 VVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFS 380

Query: 330 APVELQNGHRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGK 389
           + + + N     G+SL   L  ++ + LI   +AK  NAT   A LC+  TLD +KV GK
Sbjct: 381 SNLTI-NNQLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGK 440

Query: 390 ILVCLR-GDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIF 449
           I++C R G    V +G +A  AGA GMIL N   +G    A+PHV    +      ++  
Sbjct: 441 IVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRP 500

Query: 450 SYIKST-----KNPM--GYLIPPS---AKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTA 509
             +K+T      +P+  G  I  S        KPAP MA+FSSRGPN I P I+KPDVTA
Sbjct: 501 HGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTA 560

Query: 510 PGVNIIAAFSEAVSPTGEPFDNRT-VPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAI 569
           PGVNI+AA+SE  S +    DNR    F  + GTSMSCPH SG+ GLL+T HP WSP+AI
Sbjct: 561 PGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAI 620

Query: 570 KSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLE 629
           KSAIMT+A   DNT +P+ D     LA A  FAYGSGH+RP  AI+PGLVYDLS  DYL 
Sbjct: 621 KSAIMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSLTDYLN 680

Query: 630 FLCASGYNEKTIQAFS-DGPFKCPASASILNFNYPSIGVQNL-TGSVTVTRKLKNVSTPG 689
           FLCASGY+++ I A + +  F C  S S+ + NYPSI + NL    VT+ R + NV  P 
Sbjct: 681 FLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPS 740

Query: 690 VYKARVRHPNGVKVLVKPKVLKFERVGEEKSFELTI--TGDVPEDQVVDGVLIWTDGKHF 732
            Y    R PNG  + V P  L F ++GE K+F++ +  +      +   G L WTDGKH 
Sbjct: 741 TYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHI 786

[Close]

BLAST of Chy12G212470 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 9.1e-160
Identity = 329/709 (46.40%), Postives = 429/709 (60.51%), Query Frame = 0


Query: 32  INGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGV 91
           I+GF+  + +EEA  L              P V +VLP    +LHTT +  F+ L+++  
Sbjct: 73  IHGFSTRLTQEEADSL-----------MTQPGVISVLPEHRYELHTTRTPLFLGLDEH-- 132

Query: 92  IPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRVP-C 151
              +  +  A S  DV++  LDTGVWP SKS+ + G  GP+PS WKGGC   T      C
Sbjct: 133 --TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTASLC 192

Query: 152 NRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGLGV 211
           NRKLIGA++F +G+ + +   D  +    S RD DGHG+HT STA GS V GAS  G   
Sbjct: 193 NRKLIGARFFARGYESTMGPID-ESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 252

Query: 212 GTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPADYYDD 271
           GTA+G +P+ARVA YKVCW    GGCF +DI  A D AI D V+VLS+SLGG  +DYY D
Sbjct: 253 GTARGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 312

Query: 272 GIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNGHR 331
           G+AI AF AM++GI V CSAGN+GP + ++SN APWI TVGA T+DR+F A   L NG  
Sbjct: 313 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 372

Query: 332 YMGSSLSKG-LKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDT 391
           + G SL KG    DKL   I      A NAT     LC   TL   KVKGKI++C RG  
Sbjct: 373 FTGVSLFKGEALPDKLLPFIYA--GNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGIN 432

Query: 392 ARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKNPM 451
           ARV KG+    AG VGMIL N   +G E +AD H+LPA+ +    G  I  Y+ +  NP 
Sbjct: 433 ARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 492

Query: 452 GYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPF 511
             +      V  KP+P +AAFSSRGPN I+P I+KPD+ APGVNI+AA++ A  PTG   
Sbjct: 493 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 552

Query: 512 DNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPMLDG 571
           D+R V F  +SGTSMSCPHVSGL  LL+++HP WSP+AI+SA+MT+A       KP+LD 
Sbjct: 553 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 612

Query: 572 GSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFK 631
            +    P+TPF +G+GH+ PT A +PGL+YDL+  DYL FLCA  Y    I++ S   + 
Sbjct: 613 ATG--KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYT 672

Query: 632 CPASA--SILNFNYPSIGVQ-NLTGSVTVTRKLKNVSTPGVYKARV-RHPNGVKVLVKPK 691
           C  S   S+ + NYPS  V  +  G+   TR + +V   G Y  +V     GVK+ V+P 
Sbjct: 673 CDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 732

Query: 692 VLKFERVGEEKSFELTITGD--VPEDQVVDGVLIWTDGKHFVRSPIVVS 733
           VL F+   E+KS+ +T T D   P      G + W+DGKH V SP+ +S
Sbjct: 733 VLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

[Close]

BLAST of Chy12G212470 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 7.7e-151
Identity = 312/719 (43.39%), Postives = 424/719 (58.97%), Query Frame = 0


Query: 33  NGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVI 92
           +GF+A++  +EA            N+  HP V AV  +R ++LHTT S +F+ L+     
Sbjct: 67  HGFSAVVTPDEA-----------DNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQ--- 126

Query: 93  PPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTP-DRVPCN 152
                W  +  G DVII   DTG+WP  +SF +  + GP+P +W+G C          CN
Sbjct: 127 --KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGARFSPRNCN 186

Query: 153 RKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGLGVG 212
           RK+IGA++F KG  A +           S RD DGHG+HT STA G +   AS  G   G
Sbjct: 187 RKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASG 246

Query: 213 TAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGE---PADYY 272
            AKG +PKAR+AAYKVCW  +D GC D+DI  AFD A+ D VDV+S+S+GG     + YY
Sbjct: 247 VAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYY 306

Query: 273 DDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNG 332
            D IAI ++ A  KGI V  SAGN GP   +V+N APW+ TVGAST+DR F A   L +G
Sbjct: 307 LDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDG 366

Query: 333 HRYMGSSLSKGLK-GDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRG 392
           HR  G SL  G+    +++ ++   ++   +A+     LC   TLD  +V+GKI++C RG
Sbjct: 367 HRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVICDRG 426

Query: 393 DTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKN 452
            + RV KG     AG VGMIL N   +G   + D H++PA  +  N+G  I +Y  S  N
Sbjct: 427 SSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPN 486

Query: 453 PMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGE 512
           P+  +      V  KPAP +A+FS RGPN +SPEI+KPD+ APGVNI+AA+++AV PTG 
Sbjct: 487 PIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGL 546

Query: 513 PFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPML 572
           P D R   F  +SGTSM+CPHVSG   LL++ HP WSP+ I+SA+MT+  + DN+ + ++
Sbjct: 547 PSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLI 606

Query: 573 DGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGP 632
           D  +     ATP+ YGSGH+    A++PGLVYD++ +DY+ FLC+ GY  KTIQ  +  P
Sbjct: 607 DESTG--KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTP 666

Query: 633 FKCPAS--ASILNFNYPSIGV---QNLTG--SVTVTRKLKNV-STPGVYKARVRHPNGVK 692
            +CP +   S  N NYPSI      N  G  S TV R   NV     VY+AR+  P GV 
Sbjct: 667 VRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVT 726

Query: 693 VLVKPKVLKFERVGEEKSFELTITGD-----VPEDQVVDGVLIWTD-GKHFVRSPIVVS 733
           V VKP  L F    + +S+ +T+T +     + E   V G + W D GKH VRSPIVV+
Sbjct: 727 VTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

[Close]

BLAST of Chy12G212470 vs. ExPASy TrEMBL
Match: A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)

HSP 1 Score: 1365.9 bits (3534), Expect = 0.0e+00
Identity = 677/708 (95.62%), Postives = 687/708 (97.03%), Query Frame = 0


Query: 29  KKNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           KKNINGFAAIMDEEEA QLAK           HPEVAAVLPNRAKKLHTTHSWEFMHLEK
Sbjct: 61  KKNINGFAAIMDEEEAAQLAK-----------HPEVAAVLPNRAKKLHTTHSWEFMHLEK 120

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRV 148
           NGVIPPSSAWRRAKSGKDVIIANLDTGVWP SKSFGEHGIVGPVPSKWKGGCTDKT DRV
Sbjct: 121 NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 180

Query: 149 PCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGL 208
           PCNRKLIGAKYFNKGFLAYLKSE+LTA VINSTRDYDGHGSHTLSTAGGSYVSGAS FGL
Sbjct: 181 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 240

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPADYY 268
           GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIA+AFDHAIHDRVDVLSLSLGGEPADYY
Sbjct: 241 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY 300

Query: 269 DDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNG 328
           DDGIAI+AFHA+KKGIPVVCSAGNSGPGAQTVSNTAPWILTVGAST+DREFQAPVELQNG
Sbjct: 301 DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG 360

Query: 329 HRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGD 388
           HRYMGSSLSKGLKGDKLY LITGAEAKAKNATAE A LCKPKTLDHSKVKGKILVCLRGD
Sbjct: 361 HRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGD 420

Query: 389 TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKNP 448
           TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQA+FSYIK+TKNP
Sbjct: 421 TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNP 480

Query: 449 MGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEP 508
           MGYLIPP+AKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEP
Sbjct: 481 MGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEP 540

Query: 509 FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPMLD 568
           FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP+WSPSAIKSAIMTSARIRDNTKKPMLD
Sbjct: 541 FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLD 600

Query: 569 GGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPF 628
           GGSPDLAP+TPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPF
Sbjct: 601 GGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPF 660

Query: 629 KCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKARVRHPNGVKVLVKPKVLK 688
           KCPASASILN NYPSIGVQNLTGSVTVTRKLKNVSTPGVYK RVRHPNGVKVLVKPKVLK
Sbjct: 661 KCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLK 720

Query: 689 FERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 737
           FERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF
Sbjct: 721 FERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

[Close]

BLAST of Chy12G212470 vs. ExPASy TrEMBL
Match: A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)

HSP 1 Score: 1360.1 bits (3519), Expect = 0.0e+00
Identity = 692/787 (87.93%), Postives = 706/787 (89.71%), Query Frame = 0


Query: 1   MKHRKMKIYNSILILFSLLLIISPAMAAKK------------------------------ 60
           MKH KMKIYNSILILFSLLL+ISPA+AAKK                              
Sbjct: 1   MKHPKMKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKL 60

Query: 61  ---------------------NINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLP 120
                                NINGFAAIMDEEEATQLAK           HPEVAAVL 
Sbjct: 61  LGSIIGSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAK-----------HPEVAAVLL 120

Query: 121 NRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIV 180
           N+AKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVII NLDTGVW  SKSFGEHGIV
Sbjct: 121 NKAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIV 180

Query: 181 GPVPSKWKGGCTDKTPDRVPCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGS 240
           G VPSKWKGGCTDKTPD V CNRKLIGAKYFNKGFLAYL S++LTASVINSTRDYDGHGS
Sbjct: 181 GAVPSKWKGGCTDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLTASVINSTRDYDGHGS 240

Query: 241 HTLSTAGGSYVSGASAFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAI 300
           HTLSTAGGSYVSGAS FGLGVGTAKGGSPKARVA+YKVCWPLEDGGCF+ADIAEAFDHAI
Sbjct: 241 HTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAI 300

Query: 301 HDRVDVLSLSLGGEPADYYDDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILT 360
           HDRVDVLSLSLGGEPADYYDDGIAIAAFHA+KKGIPVVCSAGNSGP AQTVSNTAPWILT
Sbjct: 301 HDRVDVLSLSLGGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILT 360

Query: 361 VGASTVDREFQAPVELQNGHRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKP 420
           VGAST+DREFQAPVELQNGH YMGSSLSKGLKGDKLY LITGAEAKAKNATAEVAMLCKP
Sbjct: 361 VGASTLDREFQAPVELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKP 420

Query: 421 KTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASH 480
           KTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCND+LSGFETIADPHVLPASH
Sbjct: 421 KTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASH 480

Query: 481 INYNDGQAIFSYIKSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTA 540
           INYNDGQA+FSYIKSTKNPMG LIPPSAKVNTKPAP+MAAFSSRGPNLISPEIIKPDVTA
Sbjct: 481 INYNDGQAVFSYIKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTA 540

Query: 541 PGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIK 600
           PGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP WSPSAIK
Sbjct: 541 PGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIK 600

Query: 601 SAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEF 660
           SAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEF
Sbjct: 601 SAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEF 660

Query: 661 LCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYK 720
           LCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT+TRKLKNVSTPGVYK
Sbjct: 661 LCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYK 720

Query: 721 ARVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPI 737
           ARV HPNGVKVLVKPKVLKFERVGEEK FEL ITGDVPE+QVVDGVLIWTDGKHFVRSPI
Sbjct: 721 ARVMHPNGVKVLVKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPI 776

[Close]

BLAST of Chy12G212470 vs. ExPASy TrEMBL
Match: A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 591/620 (95.32%), Postives = 603/620 (97.26%), Query Frame = 0


Query: 115 GVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRVPCNRKLIGAKYFNKGFLAYLKSEDLT 174
           GVW  SKSFGEHGIVG VPSKWKGGCTDKTPD V CNRKLIGAKYFNKGFLAYL S++LT
Sbjct: 21  GVWGESKSFGEHGIVGAVPSKWKGGCTDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLT 80

Query: 175 ASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGLGVGTAKGGSPKARVAAYKVCWPLEDG 234
           ASVINSTRDYDGHGSHTLSTAGGSYVSGAS FGLGVGTAKGGSPKARVA+YKVCWPLEDG
Sbjct: 81  ASVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVASYKVCWPLEDG 140

Query: 235 GCFDADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIAAFHAMKKGIPVVCSAGNSG 294
           GCF+ADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIAAFHA+KKGIPVVCSAGNSG
Sbjct: 141 GCFEADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSG 200

Query: 295 PGAQTVSNTAPWILTVGASTVDREFQAPVELQNGHRYMGSSLSKGLKGDKLYQLITGAEA 354
           P AQTVSNTAPWILTVGAST+DREFQAPVELQNGH YMGSSLSKGLKGDKLY LITGAEA
Sbjct: 201 PAAQTVSNTAPWILTVGASTLDREFQAPVELQNGHSYMGSSLSKGLKGDKLYPLITGAEA 260

Query: 355 KAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELS 414
           KAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCND+LS
Sbjct: 261 KAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDKLS 320

Query: 415 GFETIADPHVLPASHINYNDGQAIFSYIKSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRG 474
           GFETIADPHVLPASHINYNDGQA+FSYIKSTKNPMG LIPPSAKVNTKPAP+MAAFSSRG
Sbjct: 321 GFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRG 380

Query: 475 PNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVG 534
           PNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVG
Sbjct: 381 PNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVG 440

Query: 535 LLRTLHPRWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAID 594
           LLRTLHP WSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAID
Sbjct: 441 LLRTLHPHWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAID 500

Query: 595 PGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT 654
           PGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT
Sbjct: 501 PGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT 560

Query: 655 VTRKLKNVSTPGVYKARVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDG 714
           +TRKLKNVSTPGVYKARV HPNGVKVLVKPKVLKFERVGEEK FEL ITGDVPE+QVVDG
Sbjct: 561 LTRKLKNVSTPGVYKARVMHPNGVKVLVKPKVLKFERVGEEKRFELIITGDVPENQVVDG 620

Query: 715 VLIWTDGKHFVRSPIVVSSS 735
           VLIWTDGKHFVRSPIV  +S
Sbjct: 621 VLIWTDGKHFVRSPIVRRAS 640

[Close]

BLAST of Chy12G212470 vs. ExPASy TrEMBL
Match: A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 561/712 (78.79%), Postives = 632/712 (88.76%), Query Frame = 0


Query: 29  KKNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           KKNINGFAAI++EEEA +LAK           HPEVAAVL N+ KKLHTTHSW FM LEK
Sbjct: 79  KKNINGFAAILEEEEAAELAK-----------HPEVAAVLANKGKKLHTTHSWNFMRLEK 138

Query: 89  -NGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDR 148
            NGV+PP+S W RA  G+D IIANLDTGVWP SKSFGE+GIVG VP+KWKGGCTD +PDR
Sbjct: 139 MNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDR 198

Query: 149 VPCNRKLIGAKYFNKGFLAYLKSEDLTA---SVINSTRDYDGHGSHTLSTAGGSYVSGAS 208
           VPCNRKLIGAKYFNKGF+AYLK+ + +A   S+ NSTRDY+GHGSHTLSTAGGS+V  AS
Sbjct: 199 VPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDAS 258

Query: 209 AFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEP 268
            FG G+GTAKGGSPKARVAAYK+CWP +DGGCFDADI E FDHAIHD V+V+SLS+GG P
Sbjct: 259 VFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGSP 318

Query: 269 ADYYDDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVE 328
           A+Y+DD IAIAAFHA+KKGIPVVCSAGNSGP A T SNTAPWILTVGAST+DR+FQAPVE
Sbjct: 319 ANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPVE 378

Query: 329 LQNGHRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVC 388
           L+NG R+ GSSLS  L  DKLY LITGA+AKA+NA+A  AMLCKP+TLDHSK KGKIL C
Sbjct: 379 LKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILAC 438

Query: 389 LRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKS 448
           LRGD ARVDKGEQAALAGA GMILCNDELSGFETIADPH+LPASH+NYNDGQA+  YI+S
Sbjct: 439 LRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQS 498

Query: 449 TKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSP 508
           T+NPMGYLIPP+AK+NTKPAP MAAFSSRGPN+++PEIIKPDVTAPGVN+IAA++EAVSP
Sbjct: 499 TQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVSP 558

Query: 509 TGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKK 568
           TGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LHP WSPSAIKSAIMTSARIRDNT K
Sbjct: 559 TGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMK 618

Query: 569 PMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFS 628
           PMLDGG+ +LAPATPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GY+EK I+AFS
Sbjct: 619 PMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFS 678

Query: 629 DGPFKCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKARVRHPNGVKVLVKP 688
           DGP+KCP SAS+LNFNYPSIGVQN+TGSVTVTR+LKNV TPGVY+ARVR P GV+V V+P
Sbjct: 679 DGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEP 738

Query: 689 KVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 737
           + LKF++VGEEKSF+LTI G VP  +VVDG LIWTDG+HFVRSPIV+SS LF
Sbjct: 739 RFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779

[Close]

BLAST of Chy12G212470 vs. ExPASy TrEMBL
Match: A0A6J1EGQ8 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE=3 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 560/712 (78.65%), Postives = 621/712 (87.22%), Query Frame = 0


Query: 29  KKNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           K +INGFAAI+DEEEAT+LAK           HPE+AAVLPN+AK+LHTT SWEFMHLEK
Sbjct: 53  KNHINGFAAILDEEEATKLAK-----------HPEIAAVLPNKAKELHTTRSWEFMHLEK 112

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRV 148
           NGV PPSS WR+A+SG++VIIANLDTGVWP SKSFGEHGIVG VPSKWKGGC DKTPD V
Sbjct: 113 NGVSPPSSPWRKARSGQNVIIANLDTGVWPESKSFGEHGIVGGVPSKWKGGCMDKTPDAV 172

Query: 149 PCNRKLIGAKYFNKGFLAYLKSEDLT---ASVINSTRDYDGHGSHTLSTAGGSYVSGASA 208
           PCN+KLIGAKYFN G +AYLKSE+LT   + ++NSTRDY+GHGSHTLSTAGGSYVSG S 
Sbjct: 173 PCNKKLIGAKYFNHGVIAYLKSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSGVSV 232

Query: 209 FGLGVGTAKGGSPKARVAAYKVCWPLED-GGCFDADIAEAFDHAIHDRVDVLSLSLGGEP 268
           FG G+GTAKGGSPKA VAAYKVCWPL D GGCFDADIA+AFDHAIHD VDVLS+SLG  P
Sbjct: 233 FGSGIGTAKGGSPKAHVAAYKVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSVSLGSPP 292

Query: 269 ADYYDDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVE 328
           A+YY+D IAIA+FHA+KKGIPVVCSAGN GP   TVSNTAPWILTVGAST+DREFQAP+E
Sbjct: 293 AEYYNDIIAIASFHALKKGIPVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQAPIE 352

Query: 329 LQNGHRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVC 388
           L+NG  + GSSLS  L G KLY LITGA+AKA  A+ + AMLCKPKTLDHSKVKGKILVC
Sbjct: 353 LRNGKHFKGSSLSGPLSGRKLYPLITGAQAKATTASTDDAMLCKPKTLDHSKVKGKILVC 412

Query: 389 LRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKS 448
           LRGD++RVDKG QA LAGAVGMILCND LSGFE IADPHVLPASHI+YNDGQA+ SYI S
Sbjct: 413 LRGDSSRVDKGVQALLAGAVGMILCNDRLSGFEIIADPHVLPASHISYNDGQAVSSYINS 472

Query: 449 TKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSP 508
           TKNPMG+LIPP +KVNTKP+PTMAAFSSRGPN++SPEIIKPDVTAPGVNIIAAFS +VSP
Sbjct: 473 TKNPMGHLIPPLSKVNTKPSPTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGSVSP 532

Query: 509 TGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKK 568
           TGEPFDNRTVP+ITMSGTSMSCPHVSG+VGL++ LHP+WSP+AIKSAIMTSARIRDNT  
Sbjct: 533 TGEPFDNRTVPYITMSGTSMSCPHVSGIVGLVKALHPKWSPAAIKSAIMTSARIRDNTMN 592

Query: 569 PMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFS 628
            MLDGGSP  A AT F +GSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY EK ++ F+
Sbjct: 593 IMLDGGSPIFASATAFIFGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNLRVFA 652

Query: 629 DGPFKCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKARVRHPNGVKVLVKP 688
           DG FKCP S+SILNFNYPSIGVQNLTG+VT+TR+LKNV  PGVY+ RV+ P GVKV VKP
Sbjct: 653 DGNFKCPVSSSILNFNYPSIGVQNLTGNVTLTRRLKNVGRPGVYRVRVQQPEGVKVSVKP 712

Query: 689 KVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 737
            VLKFE++GEEK FELT+TG V   Q+  G LIWTDGKH VRSPIVVSS  F
Sbjct: 713 SVLKFEKIGEEKRFELTMTGVVANGQIGYGTLIWTDGKHLVRSPIVVSSGFF 753

[Close]

BLAST of Chy12G212470 vs. NCBI nr
Match: XP_031739111.1 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 1360 bits (3519), Expect = 0.0
Identity = 677/708 (95.62%), Postives = 687/708 (97.03%), Query Frame = 0


Query: 29  KKNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           KKNINGFAAIMDEEEA QLAK           HPEVAAVLPNRAKKLHTTHSWEFMHLEK
Sbjct: 61  KKNINGFAAIMDEEEAAQLAK-----------HPEVAAVLPNRAKKLHTTHSWEFMHLEK 120

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRV 148
           NGVIPPSSAWRRAKSGKDVIIANLDTGVWP SKSFGEHGIVGPVPSKWKGGCTDKT DRV
Sbjct: 121 NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 180

Query: 149 PCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGL 208
           PCNRKLIGAKYFNKGFLAYLKSE+LTA VINSTRDYDGHGSHTLSTAGGSYVSGAS FGL
Sbjct: 181 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 240

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPADYY 268
           GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIA+AFDHAIHDRVDVLSLSLGGEPADYY
Sbjct: 241 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY 300

Query: 269 DDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNG 328
           DDGIAI+AFHA+KKGIPVVCSAGNSGPGAQTVSNTAPWILTVGAST+DREFQAPVELQNG
Sbjct: 301 DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG 360

Query: 329 HRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGD 388
           HRYMGSSLSKGLKGDKLY LITGAEAKAKNATAE A LCKPKTLDHSKVKGKILVCLRGD
Sbjct: 361 HRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGD 420

Query: 389 TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKNP 448
           TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQA+FSYIK+TKNP
Sbjct: 421 TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNP 480

Query: 449 MGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEP 508
           MGYLIPP+AKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEP
Sbjct: 481 MGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEP 540

Query: 509 FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPMLD 568
           FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP+WSPSAIKSAIMTSARIRDNTKKPMLD
Sbjct: 541 FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLD 600

Query: 569 GGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPF 628
           GGSPDLAP+TPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPF
Sbjct: 601 GGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPF 660

Query: 629 KCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKARVRHPNGVKVLVKPKVLK 688
           KCPASASILN NYPSIGVQNLTGSVTVTRKLKNVSTPGVYK RVRHPNGVKVLVKPKVLK
Sbjct: 661 KCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLK 720

Query: 689 FERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 736
           FERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF
Sbjct: 721 FERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

[Close]

BLAST of Chy12G212470 vs. NCBI nr
Match: KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])

HSP 1 Score: 1360 bits (3519), Expect = 0.0
Identity = 677/708 (95.62%), Postives = 687/708 (97.03%), Query Frame = 0


Query: 29  KKNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           KKNINGFAAIMDEEEA QLAK           HPEVAAVLPNRAKKLHTTHSWEFMHLEK
Sbjct: 57  KKNINGFAAIMDEEEAAQLAK-----------HPEVAAVLPNRAKKLHTTHSWEFMHLEK 116

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRV 148
           NGVIPPSSAWRRAKSGKDVIIANLDTGVWP SKSFGEHGIVGPVPSKWKGGCTDKT DRV
Sbjct: 117 NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 176

Query: 149 PCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGL 208
           PCNRKLIGAKYFNKGFLAYLKSE+LTA VINSTRDYDGHGSHTLSTAGGSYVSGAS FGL
Sbjct: 177 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 236

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPADYY 268
           GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIA+AFDHAIHDRVDVLSLSLGGEPADYY
Sbjct: 237 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY 296

Query: 269 DDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNG 328
           DDGIAI+AFHA+KKGIPVVCSAGNSGPGAQTVSNTAPWILTVGAST+DREFQAPVELQNG
Sbjct: 297 DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG 356

Query: 329 HRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGD 388
           HRYMGSSLSKGLKGDKLY LITGAEAKAKNATAE A LCKPKTLDHSKVKGKILVCLRGD
Sbjct: 357 HRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGD 416

Query: 389 TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKNP 448
           TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQA+FSYIK+TKNP
Sbjct: 417 TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNP 476

Query: 449 MGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEP 508
           MGYLIPP+AKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEP
Sbjct: 477 MGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEP 536

Query: 509 FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPMLD 568
           FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP+WSPSAIKSAIMTSARIRDNTKKPMLD
Sbjct: 537 FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLD 596

Query: 569 GGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPF 628
           GGSPDLAP+TPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPF
Sbjct: 597 GGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPF 656

Query: 629 KCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKARVRHPNGVKVLVKPKVLK 688
           KCPASASILN NYPSIGVQNLTGSVTVTRKLKNVSTPGVYK RVRHPNGVKVLVKPKVLK
Sbjct: 657 KCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLK 716

Query: 689 FERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 736
           FERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF
Sbjct: 717 FERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 753

[Close]

BLAST of Chy12G212470 vs. NCBI nr
Match: XP_008464322.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo])

HSP 1 Score: 1353 bits (3503), Expect = 0.0
Identity = 692/787 (87.93%), Postives = 706/787 (89.71%), Query Frame = 0


Query: 1   MKHRKMKIYNSILILFSLLLIISPAMAAKK------------------------------ 60
           MKH KMKIYNSILILFSLLL+ISPA+AAKK                              
Sbjct: 1   MKHPKMKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKL 60

Query: 61  ---------------------NINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLP 120
                                NINGFAAIMDEEEATQLAK           HPEVAAVL 
Sbjct: 61  LGSIIGSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAK-----------HPEVAAVLL 120

Query: 121 NRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIV 180
           N+AKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVII NLDTGVW  SKSFGEHGIV
Sbjct: 121 NKAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIV 180

Query: 181 GPVPSKWKGGCTDKTPDRVPCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGS 240
           G VPSKWKGGCTDKTPD V CNRKLIGAKYFNKGFLAYL S++LTASVINSTRDYDGHGS
Sbjct: 181 GAVPSKWKGGCTDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLTASVINSTRDYDGHGS 240

Query: 241 HTLSTAGGSYVSGASAFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAI 300
           HTLSTAGGSYVSGAS FGLGVGTAKGGSPKARVA+YKVCWPLEDGGCF+ADIAEAFDHAI
Sbjct: 241 HTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAI 300

Query: 301 HDRVDVLSLSLGGEPADYYDDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILT 360
           HDRVDVLSLSLGGEPADYYDDGIAIAAFHA+KKGIPVVCSAGNSGP AQTVSNTAPWILT
Sbjct: 301 HDRVDVLSLSLGGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILT 360

Query: 361 VGASTVDREFQAPVELQNGHRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKP 420
           VGAST+DREFQAPVELQNGH YMGSSLSKGLKGDKLY LITGAEAKAKNATAEVAMLCKP
Sbjct: 361 VGASTLDREFQAPVELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKP 420

Query: 421 KTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASH 480
           KTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCND+LSGFETIADPHVLPASH
Sbjct: 421 KTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASH 480

Query: 481 INYNDGQAIFSYIKSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTA 540
           INYNDGQA+FSYIKSTKNPMG LIPPSAKVNTKPAP+MAAFSSRGPNLISPEIIKPDVTA
Sbjct: 481 INYNDGQAVFSYIKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTA 540

Query: 541 PGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIK 600
           PGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP WSPSAIK
Sbjct: 541 PGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIK 600

Query: 601 SAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEF 660
           SAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEF
Sbjct: 601 SAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEF 660

Query: 661 LCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYK 720
           LCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT+TRKLKNVSTPGVYK
Sbjct: 661 LCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYK 720

Query: 721 ARVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPI 736
           ARV HPNGVKVLVKPKVLKFERVGEEK FEL ITGDVPE+QVVDGVLIWTDGKHFVRSPI
Sbjct: 721 ARVMHPNGVKVLVKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPI 776

[Close]

BLAST of Chy12G212470 vs. NCBI nr
Match: XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1257 bits (3252), Expect = 0.0
Identity = 641/784 (81.76%), Postives = 678/784 (86.48%), Query Frame = 0


Query: 6   MKIYNSILILFSLLLIISPAMAAKKN---------------------------------- 65
           MKI NSILILFS LL+IS A+AAKK+                                  
Sbjct: 1   MKISNSILILFSFLLLISSAIAAKKSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFL 60

Query: 66  -----------------INGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKK 125
                            INGFAA +DEEEAT+LA           EHPEVAA+L NRAK 
Sbjct: 61  GSEEKAKDAIFYSYKKHINGFAATLDEEEATKLA-----------EHPEVAAILANRAKA 120

Query: 126 LHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPS 185
           LHTTHSWEFMHLEKNGVIP SS WR AKSGKDVIIANLDTGVWP SKSFGEHGIVGPVPS
Sbjct: 121 LHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPS 180

Query: 186 KWKGGCTDKTPDRVPCNRKLIGAKYFNKGFLAYLKSEDLT--ASVINSTRDYDGHGSHTL 245
           KWKGGC D+TPDRVPCNRKLIGAKYFNKGF+AYLKSE+ T  +SVINSTRDY+GHGSHTL
Sbjct: 181 KWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYLKSENSTEFSSVINSTRDYEGHGSHTL 240

Query: 246 STAGGSYVSGASAFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDR 305
           STAGGSYVSGAS FG G GTAKGGSPKARVAAYKVCWPLE GGCFD+DI EAFDHAIHD 
Sbjct: 241 STAGGSYVSGASVFGSGAGTAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDG 300

Query: 306 VDVLSLSLGGEPADYYDDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGA 365
           VDVLSLSLG +PA+Y +D IAIAAFHA+KKGIPVVCSAGNSGP A T SNTAPWILTVGA
Sbjct: 301 VDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGA 360

Query: 366 STVDREFQAPVELQNGHRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTL 425
           ST+DREFQAPVELQNGHRYMGSSLSK L+G+KLY LITGA+AKAKNA  + AMLCKP+TL
Sbjct: 361 STLDREFQAPVELQNGHRYMGSSLSKALQGNKLYPLITGAQAKAKNAAMDDAMLCKPETL 420

Query: 426 DHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINY 485
           DHSKVKGKIL CLRGD ARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINY
Sbjct: 421 DHSKVKGKILACLRGDNARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINY 480

Query: 486 NDGQAIFSYIKSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGV 545
           NDGQA+ SYI STKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPN+ISPEIIKPDVTAPGV
Sbjct: 481 NDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGV 540

Query: 546 NIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAI 605
           N+IAAFSEAVSPTGE FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP WSPSAIKSAI
Sbjct: 541 NVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAI 600

Query: 606 MTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 665
           MTSARIRDNT KPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA
Sbjct: 601 MTSARIRDNTMKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 660

Query: 666 SGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKARV 725
           SGY++KTI+AFSDGPFKCP +ASILNFNYPSIGVQNL G+VTVTRKLKNVSTPGVY+AR+
Sbjct: 661 SGYDDKTIRAFSDGPFKCPPNASILNFNYPSIGVQNLNGNVTVTRKLKNVSTPGVYRARI 720

Query: 726 RHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVS 736
            +P+GVKV VKPKVLKFERV EEKSFELT+TG+VPEDQVVDGVLIWTDGKHFVRSPIV+S
Sbjct: 721 MNPDGVKVSVKPKVLKFERVAEEKSFELTMTGNVPEDQVVDGVLIWTDGKHFVRSPIVIS 773

[Close]

BLAST of Chy12G212470 vs. NCBI nr
Match: TYK15479.1 (subtilisin-like protease SBT5.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1190 bits (3078), Expect = 0.0
Identity = 591/620 (95.32%), Postives = 603/620 (97.26%), Query Frame = 0


Query: 115 GVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRVPCNRKLIGAKYFNKGFLAYLKSEDLT 174
           GVW  SKSFGEHGIVG VPSKWKGGCTDKTPD V CNRKLIGAKYFNKGFLAYL S++LT
Sbjct: 21  GVWGESKSFGEHGIVGAVPSKWKGGCTDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLT 80

Query: 175 ASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGLGVGTAKGGSPKARVAAYKVCWPLEDG 234
           ASVINSTRDYDGHGSHTLSTAGGSYVSGAS FGLGVGTAKGGSPKARVA+YKVCWPLEDG
Sbjct: 81  ASVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVASYKVCWPLEDG 140

Query: 235 GCFDADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIAAFHAMKKGIPVVCSAGNSG 294
           GCF+ADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIAAFHA+KKGIPVVCSAGNSG
Sbjct: 141 GCFEADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSG 200

Query: 295 PGAQTVSNTAPWILTVGASTVDREFQAPVELQNGHRYMGSSLSKGLKGDKLYQLITGAEA 354
           P AQTVSNTAPWILTVGAST+DREFQAPVELQNGH YMGSSLSKGLKGDKLY LITGAEA
Sbjct: 201 PAAQTVSNTAPWILTVGASTLDREFQAPVELQNGHSYMGSSLSKGLKGDKLYPLITGAEA 260

Query: 355 KAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELS 414
           KAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCND+LS
Sbjct: 261 KAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDKLS 320

Query: 415 GFETIADPHVLPASHINYNDGQAIFSYIKSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRG 474
           GFETIADPHVLPASHINYNDGQA+FSYIKSTKNPMG LIPPSAKVNTKPAP+MAAFSSRG
Sbjct: 321 GFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRG 380

Query: 475 PNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVG 534
           PNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVG
Sbjct: 381 PNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVG 440

Query: 535 LLRTLHPRWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAID 594
           LLRTLHP WSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAID
Sbjct: 441 LLRTLHPHWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAID 500

Query: 595 PGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT 654
           PGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT
Sbjct: 501 PGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT 560

Query: 655 VTRKLKNVSTPGVYKARVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDG 714
           +TRKLKNVSTPGVYKARV HPNGVKVLVKPKVLKFERVGEEK FEL ITGDVPE+QVVDG
Sbjct: 561 LTRKLKNVSTPGVYKARVMHPNGVKVLVKPKVLKFERVGEEKRFELIITGDVPENQVVDG 620

Query: 715 VLIWTDGKHFVRSPIVVSSS 734
           VLIWTDGKHFVRSPIV  +S
Sbjct: 621 VLIWTDGKHFVRSPIVRRAS 640

[Close]

BLAST of Chy12G212470 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 844.7 bits (2181), Expect = 5.5e-245
Identity = 429/709 (60.51%), Postives = 520/709 (73.34%), Query Frame = 0


Query: 29  KKNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           K++INGFAAI+DE EA ++AK           HP+V +V PN+ +KLHTTHSW FM L K
Sbjct: 90  KRHINGFAAILDENEAAEIAK-----------HPDVVSVFPNKGRKLHTTHSWNFMLLAK 149

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRV 148
           NGV+  SS W +A  G+D IIANLDTGVWP SKSF + G  G VP++WKG C       V
Sbjct: 150 NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD----V 209

Query: 149 PCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGL 208
           PCNRKLIGA+YFNKG+LAY      + +   + RD+DGHGSHTLSTA G++V GA+ FG+
Sbjct: 210 PCNRKLIGARYFNKGYLAYTGLP--SNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGI 269

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAEAFDHAIHDRVDVLSLSLGGEPADY 268
           G GTA GGSPKARVAAYKVCWP  DG  CFDADI  A + AI D VDVLS S+GG+  DY
Sbjct: 270 GNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDY 329

Query: 269 YDDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQN 328
             DGIAI +FHA+K G+ VVCSAGNSGP + TVSN APW++TVGAS++DREFQA VEL+N
Sbjct: 330 MSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKN 389

Query: 329 GHRYMGSSLSKGLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRG 388
           G  + G+SLSK L  +K+Y LI+ A+A   N     A+LCK  +LD  KVKGKILVCLRG
Sbjct: 390 GQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRG 449

Query: 389 DTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKN 448
           D ARVDKG QAA AGA GM+LCND+ SG E I+D HVLPAS I+Y DG+ +FSY+ STK+
Sbjct: 450 DNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKD 509

Query: 449 PMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGE 508
           P GY+  P+A +NTKPAP MA+FSSRGPN I+P I+KPD+TAPGVNIIAAF+EA  PT  
Sbjct: 510 PKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDL 569

Query: 509 PFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPML 568
             DNR  PF T SGTSMSCPH+SG+VGLL+TLHP WSP+AI+SAIMT++R R+N +KPM+
Sbjct: 570 DSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMV 629

Query: 569 DGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGP 628
           D        A PF+YGSGH++P  A  PGLVYDL+  DYL+FLCA GYN   +Q F++ P
Sbjct: 630 D---ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDP 689

Query: 629 -FKCPASASILNFNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKARVRHPNGVKVLVKPKV 688
            + C   A++L+FNYPSI V NLTGS+TVTRKLKNV  P  Y AR R P GV+V V+PK 
Sbjct: 690 QYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQ 749

Query: 689 LKFERVGEEKSFELTITG-DVPEDQVVDGVLIWTDGKHFVRSPIVVSSS 735
           L F + GE K F++T+    V     V G L WTD  H+VRSPIVV  S
Sbjct: 750 LTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLS 777

[Close]

BLAST of Chy12G212470 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 738.8 bits (1906), Expect = 4.2e-213
Identity = 382/709 (53.88%), Postives = 492/709 (69.39%), Query Frame = 0


Query: 30  KNINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEKN 89
           K+INGFAA +D + A +++K           HPEV +V PN+A KLHTT SW+F+ LE N
Sbjct: 81  KHINGFAAHLDHDLAYEISK-----------HPEVVSVFPNKALKLHTTRSWDFLGLEHN 140

Query: 90  GVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRVP 149
             +P SS WR+A+ G+D IIANLDTGVWP SKSF + G+ GP+PS+WKG C ++      
Sbjct: 141 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATFH 200

Query: 150 CNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGLG 209
           CNRKLIGA+YFNKG+ A +   +   S  +S RD DGHGSHTLSTA G +V G S FG G
Sbjct: 201 CNRKLIGARYFNKGYAAAVGHLN---SSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQG 260

Query: 210 VGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAEAFDHAIHDRVDVLSLSLGGEPADYY 269
            GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHD  DV+S+SLGGEP  ++
Sbjct: 261 NGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFF 320

Query: 270 DDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNG 329
           +D +AI +FHA KK I VVCSAGNSGP   TVSN APW +TVGAST+DREF + + L NG
Sbjct: 321 NDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNG 380

Query: 330 HRYMGSSLSK-GLKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRG 389
             Y G SLS   L   K Y ++    AKAKNA+A  A LCK  +LD  K KGKILVCLRG
Sbjct: 381 KHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRG 440

Query: 390 DTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKN 449
              RV+KG   AL G +GM+L N  ++G + +ADPHVLPA+ +   D  A+  YI  TK 
Sbjct: 441 QNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKK 500

Query: 450 PMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGE 509
           P+ ++ P    +  KPAP MA+FSS+GP++++P+I+KPD+TAPGV++IAA++ AVSPT E
Sbjct: 501 PIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNE 560

Query: 510 PFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPML 569
            FD R + F  +SGTSMSCPH+SG+ GLL+T +P WSP+AI+SAIMT+A I D+   P+ 
Sbjct: 561 QFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQ 620

Query: 570 DGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGP 629
           +  +     ATPF++G+GH++P  A++PGLVYDL   DYL FLC+ GYN   I  FS   
Sbjct: 621 NATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNN 680

Query: 630 FKCPA-SASILNFNYPSIGVQNLTGS-VTVTRKLKNVSTPGVYKARVRHPNGVKVLVKPK 689
           F C +   S++N NYPSI V NLT S VTV+R +KNV  P +Y  +V +P GV V VKP 
Sbjct: 681 FTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 740

Query: 690 VLKFERVGEEKSFELTIT---GDVPEDQVVDGVLIWTDGKHFVRSPIVV 732
            L F +VGE+K+F++ +    G+V +  V  G L+W+D KH VRSPIVV
Sbjct: 741 SLNFTKVGEQKTFKVILVKSKGNVAKGYVF-GELVWSDKKHRVRSPIVV 770

[Close]

BLAST of Chy12G212470 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 565.5 bits (1456), Expect = 6.4e-161
Identity = 329/709 (46.40%), Postives = 429/709 (60.51%), Query Frame = 0


Query: 32  INGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGV 91
           I+GF+  + +EEA  L              P V +VLP    +LHTT +  F+ L+++  
Sbjct: 73  IHGFSTRLTQEEADSL-----------MTQPGVISVLPEHRYELHTTRTPLFLGLDEH-- 132

Query: 92  IPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTPDRVP-C 151
              +  +  A S  DV++  LDTGVWP SKS+ + G  GP+PS WKGGC   T      C
Sbjct: 133 --TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTASLC 192

Query: 152 NRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGLGV 211
           NRKLIGA++F +G+ + +   D  +    S RD DGHG+HT STA GS V GAS  G   
Sbjct: 193 NRKLIGARFFARGYESTMGPID-ESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 252

Query: 212 GTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPADYYDD 271
           GTA+G +P+ARVA YKVCW    GGCF +DI  A D AI D V+VLS+SLGG  +DYY D
Sbjct: 253 GTARGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 312

Query: 272 GIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNGHR 331
           G+AI AF AM++GI V CSAGN+GP + ++SN APWI TVGA T+DR+F A   L NG  
Sbjct: 313 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 372

Query: 332 YMGSSLSKG-LKGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDT 391
           + G SL KG    DKL   I      A NAT     LC   TL   KVKGKI++C RG  
Sbjct: 373 FTGVSLFKGEALPDKLLPFIYA--GNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGIN 432

Query: 392 ARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKNPM 451
           ARV KG+    AG VGMIL N   +G E +AD H+LPA+ +    G  I  Y+ +  NP 
Sbjct: 433 ARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 492

Query: 452 GYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPF 511
             +      V  KP+P +AAFSSRGPN I+P I+KPD+ APGVNI+AA++ A  PTG   
Sbjct: 493 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 552

Query: 512 DNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPMLDG 571
           D+R V F  +SGTSMSCPHVSGL  LL+++HP WSP+AI+SA+MT+A       KP+LD 
Sbjct: 553 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 612

Query: 572 GSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFK 631
            +    P+TPF +G+GH+ PT A +PGL+YDL+  DYL FLCA  Y    I++ S   + 
Sbjct: 613 ATG--KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYT 672

Query: 632 CPASA--SILNFNYPSIGVQ-NLTGSVTVTRKLKNVSTPGVYKARV-RHPNGVKVLVKPK 691
           C  S   S+ + NYPS  V  +  G+   TR + +V   G Y  +V     GVK+ V+P 
Sbjct: 673 CDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 732

Query: 692 VLKFERVGEEKSFELTITGD--VPEDQVVDGVLIWTDGKHFVRSPIVVS 733
           VL F+   E+KS+ +T T D   P      G + W+DGKH V SP+ +S
Sbjct: 733 VLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

[Close]

BLAST of Chy12G212470 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 535.8 bits (1379), Expect = 5.5e-152
Identity = 312/719 (43.39%), Postives = 424/719 (58.97%), Query Frame = 0


Query: 33  NGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVI 92
           +GF+A++  +EA            N+  HP V AV  +R ++LHTT S +F+ L+     
Sbjct: 67  HGFSAVVTPDEA-----------DNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQ--- 126

Query: 93  PPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTP-DRVPCN 152
                W  +  G DVII   DTG+WP  +SF +  + GP+P +W+G C          CN
Sbjct: 127 --KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGARFSPRNCN 186

Query: 153 RKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGLGVG 212
           RK+IGA++F KG  A +           S RD DGHG+HT STA G +   AS  G   G
Sbjct: 187 RKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASG 246

Query: 213 TAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGE---PADYY 272
            AKG +PKAR+AAYKVCW  +D GC D+DI  AFD A+ D VDV+S+S+GG     + YY
Sbjct: 247 VAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYY 306

Query: 273 DDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNG 332
            D IAI ++ A  KGI V  SAGN GP   +V+N APW+ TVGAST+DR F A   L +G
Sbjct: 307 LDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDG 366

Query: 333 HRYMGSSLSKGLK-GDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRG 392
           HR  G SL  G+    +++ ++   ++   +A+     LC   TLD  +V+GKI++C RG
Sbjct: 367 HRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVICDRG 426

Query: 393 DTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKN 452
            + RV KG     AG VGMIL N   +G   + D H++PA  +  N+G  I +Y  S  N
Sbjct: 427 SSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPN 486

Query: 453 PMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGE 512
           P+  +      V  KPAP +A+FS RGPN +SPEI+KPD+ APGVNI+AA+++AV PTG 
Sbjct: 487 PIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGL 546

Query: 513 PFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPML 572
           P D R   F  +SGTSM+CPHVSG   LL++ HP WSP+ I+SA+MT+  + DN+ + ++
Sbjct: 547 PSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLI 606

Query: 573 DGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGP 632
           D  +     ATP+ YGSGH+    A++PGLVYD++ +DY+ FLC+ GY  KTIQ  +  P
Sbjct: 607 DESTG--KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTP 666

Query: 633 FKCPAS--ASILNFNYPSIGV---QNLTG--SVTVTRKLKNV-STPGVYKARVRHPNGVK 692
            +CP +   S  N NYPSI      N  G  S TV R   NV     VY+AR+  P GV 
Sbjct: 667 VRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVT 726

Query: 693 VLVKPKVLKFERVGEEKSFELTITGD-----VPEDQVVDGVLIWTD-GKHFVRSPIVVS 733
           V VKP  L F    + +S+ +T+T +     + E   V G + W D GKH VRSPIVV+
Sbjct: 727 VTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

[Close]

BLAST of Chy12G212470 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 523.9 bits (1348), Expect = 2.1e-148
Identity = 316/718 (44.01%), Postives = 410/718 (57.10%), Query Frame = 0


Query: 31  NINGFAAIMDEEEATQLAKTKLENYKNVTEHPEVAAVLPNRAKKLHTTHSWEFMHLEKN- 90
           + +GF+A +D  EA  L          ++    +  +  +    LHTT + EF+ L    
Sbjct: 67  SFHGFSAYLDSTEADSL----------LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEF 126

Query: 91  GVIPPSSAWRRAKSGKDVIIANLDTGVWPGSKSFGEHGIVGPVPSKWKGGCTDKTP-DRV 150
           GV    S      S   VII  LDTGVWP S+SF +   +  +PSKWKG C   +  D  
Sbjct: 127 GVHDLGS------SSNGVIIGVLDTGVWPESRSFDDTD-MPEIPSKWKGECESGSDFDSK 186

Query: 151 PCNRKLIGAKYFNKGFLAYLKSEDLTASVINSTRDYDGHGSHTLSTAGGSYVSGASAFGL 210
            CN+KLIGA+ F+KGF         +     S RD DGHG+HT +TA GS V  AS  G 
Sbjct: 187 LCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGY 246

Query: 211 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPADYY 270
             GTA+G + +ARVA YKVCW     GCF +DI  A D AI D VDVLSLSLGG  A YY
Sbjct: 247 AAGTARGMATRARVATYKVCW---STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYY 306

Query: 271 DDGIAIAAFHAMKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTVDREFQAPVELQNG 330
            D IAI AF AM++G+ V CSAGNSGP   +V+N APW++TVGA T+DR+F A   L NG
Sbjct: 307 RDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNG 366

Query: 331 HRYMGSSLSKGL-KGDKLYQLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRG 390
            R  G SL  G+  G K  +L+           +  + LC P +LD S V+GKI+VC RG
Sbjct: 367 KRLTGVSLYSGVGMGTKPLELV------YNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRG 426

Query: 391 DTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAIFSYIKSTKN 450
             ARV+KG     AG +GMI+ N   SG E +AD H+LPA  +    G  +  Y+KS   
Sbjct: 427 VNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSK 486

Query: 451 PMGYLIPPSAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGE 510
           P   L+     ++ KP+P +AAFSSRGPN ++PEI+KPDV  PGVNI+A +S+A+ PTG 
Sbjct: 487 PTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGL 546

Query: 511 PFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPRWSPSAIKSAIMTSARIRDNTKKPML 570
             D+R   F  MSGTSMSCPH+SGL GLL+  HP WSPSAIKSA+MT+A + DNT  P+ 
Sbjct: 547 DKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLH 606

Query: 571 DGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGP 630
           D     L  + P+A+GSGH+ P  A+ PGLVYD+S  +Y+ FLC+  Y    I A    P
Sbjct: 607 DAADNSL--SNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRP 666

Query: 631 -FKCPASASIL-NFNYPSIGVQNLTGSVTVTRKLKNVSTPG----VYKARVRHPNGVKVL 690
              C    S     NYPS  V  L G   V R  + V+  G    VYK  V     V + 
Sbjct: 667 SVNCSKKFSDPGQLNYPSFSV--LFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGIS 726

Query: 691 VKPKVLKFERVGEEKSFELTIT---GDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 737
           VKP  L F+ VGE+K + +T     G    ++   G + W++ +H VRSP+  S + F
Sbjct: 727 VKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

The following BLAST results are available for this feature:

BLAST of Chy12G212470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JXC5	7.7e-244	60.51	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
Q9ZSP5	6.0e-212	53.88	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
I1N462	6.5e-166	47.04	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
O65351	9.1e-160	46.40	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
O49607	7.7e-151	43.39	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S...	[more]

BLAST of Chy12G212470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVY8	0.0e+00	95.62	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1	[more]
A0A1S3CLB7	0.0e+00	87.93	subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=...	[more]
A0A5D3CZ66	0.0e+00	95.32	Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A6J1CN43	0.0e+00	78.79	subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 P...	[more]
A0A6J1EGQ8	0.0e+00	78.65	subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE...	[more]

BLAST of Chy12G212470 vs. NCBI nr


Match Name	Identity	Description
XP_031739111.1	95.62	subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]
KAE8652826.1	95.62	hypothetical protein Csa_022873 [Cucumis sativus]	[more]
XP_008464322.1	87.93	PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo]	[more]
XP_038895602.1	81.76	subtilisin-like protease SBT5.4 [Benincasa hispida]	[more]
TYK15479.1	95.32	subtilisin-like protease SBT5.4 [Cucumis melo var. makuwa]	[more]

BLAST of Chy12G212470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59810.1	5.5e-245	60.51	Subtilase family protein	[more]
AT2G04160.1	4.2e-213	53.88	Subtilisin-like serine endopeptidase family protein	[more]
AT5G67360.1	6.4e-161	46.40	Subtilase family protein	[more]
AT4G34980.1	5.5e-152	43.39	subtilisin-like serine protease 2	[more]
AT2G05920.1	2.1e-148	44.01	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PRINTS	PR00723	SUBTILISIN	coord: 183..196 score: 33.16 coord: 520..536 score: 51.76 coord: 104..123 score: 32.19
None	No IPR available	GENE3D	3.50.30.30		coord: 316..460 e-value: 1.2E-179 score: 600.0
None	No IPR available	GENE3D	2.60.40.2310		coord: 610..734 e-value: 1.6E-36 score: 126.9
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 29..733
None	No IPR available	PANTHER	PTHR43806:SF40	BNAC02G33520D PROTEIN	coord: 29..733
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 80..593 score: 28.148706
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 323..452 e-value: 9.05384E-36 score: 129.456
None	No IPR available	SUPERFAMILY	52025	PA domain	coord: 374..445
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 104..562 e-value: 9.6E-43 score: 146.9
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 638..731 e-value: 2.0E-26 score: 92.1
IPR003137	PA domain	PFAM	PF02225	PA	coord: 367..438 e-value: 5.0E-11 score: 42.5
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 106..606 e-value: 1.2E-179 score: 600.0
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 75..597
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 521..531
IPR034197	Cucumisin-like catalytic domain	CDD	cd04852	Peptidases_S8_3	coord: 73..556 e-value: 4.46654E-130 score: 386.183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G212470.1	Chy12G212470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008236	serine-type peptidase activity