Chy12G212230 (gene) Cucumber (hystrix) v1

BLAST of Chy12G212230 vs. ExPASy Swiss-Prot
Match: O14214 (tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=trm10 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.1e-35
Identity = 111/302 (36.75%), Postives = 172/302 (56.95%), Query Frame = 0

Query: 25  EPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLASVTEEERAKLI 84
           +P LSK+A K+L +QQ ++A + +K+ + + +KK+               + +EER + I
Sbjct: 29  QPVLSKSALKRLKRQQEWDAGR-EKRAEMRREKKR---------------LRKEERKRKI 88

Query: 85  ESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVIDLEFSHLMNPSEIHSLVQQIMYC 144
           E+         G+  + ++K+I RL         IV+D  F  LMN  EI+SL QQ+  C
Sbjct: 89  EA---------GEVVKSQKKRI-RLGKVVPSSIRIVLDCAFDDLMNDKEINSLCQQVTRC 148

Query: 145 YAVNGRCPSPCHLWLTGCNGEMET-QLRRLPG-FDKW-IIEKERRSYIDALQEQKENLVY 204
           ++ N     P  L+ T   G ++T Q   L G  + W       +SY++  + QKE LVY
Sbjct: 149 HSANRTALHPVELFATNFGGRLKTRQDFVLKGQQNNWKRYNPTTKSYLEEFESQKEKLVY 208

Query: 205 LTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQEIQTAKLPIGSYLKMSSSQV 264
           L+ADS+  + +LD  KIYI+G +VD+NR+K +   KA EQ I+TAKLPI  Y+K++  ++
Sbjct: 209 LSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNKASEQGIKTAKLPIDEYIKITDRKI 268

Query: 265 LTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNSDHPVD-GEENGDGDNKNETKK 323
           LTVNQV EIL  +LE RDW+ +F EV+P+RK  G    SD   D  E+     N+++++ 
Sbjct: 269 LTVNQVFEILSLWLEYRDWEKAFMEVIPKRK--GILLKSDESFDVSEDTRSQSNQSDSEL 302

BLAST of Chy12G212230 vs. ExPASy Swiss-Prot
Match: Q8C1Z8 (tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 3.5e-34
Identity = 100/290 (34.48%), Postives = 155/290 (53.45%), Query Frame = 0

Query: 5   EEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERK 64
           EE   P++D             P+SK   KKL+KQ+++E ++  +K + KE++K      
Sbjct: 26  EERQEPRVDAGAE---------PISKRQLKKLMKQKQWEEQREQRKEKRKEKRK------ 85

Query: 65  RKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVIDLE 124
                                 R+ L + ++   S+  ++K  R   AR     +VID  
Sbjct: 86  ----------------------RKKLERRQLESNSDGNDRKRVRRDVARS-SLRLVIDCS 145

Query: 125 FSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQL-RRLPGFDKW-IIE 184
           F  LM   +I  L +QI  CYA N R   P   +LT   G+++  +     G+  W  I 
Sbjct: 146 FDDLMVLKDIKKLHKQIQRCYAENRRASHPVQFYLTSHGGQLKKNMDENDQGWVNWKDIH 205

Query: 185 KERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQE 244
            +   Y + +  +KE+LVYLT+DS  VL DLD  K Y++GGLVD +  KG+T K+A    
Sbjct: 206 IKSEHYSELI--KKEDLVYLTSDSPNVLKDLDESKAYVIGGLVDHSHHKGLTFKQATSYG 265

Query: 245 IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRK 293
           I+ A+LP+  ++KM+S +VL VN V EI+L++LETRDW+++FF ++PQRK
Sbjct: 266 IEHAQLPLADFVKMNSRKVLAVNHVFEIILEFLETRDWQEAFFTILPQRK 275

BLAST of Chy12G212230 vs. ExPASy Swiss-Prot
Match: Q8TBZ6 (tRNA methyltransferase 10 homolog A OS=Homo sapiens OX=9606 GN=TRMT10A PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.6e-33
Identity = 110/331 (33.23%), Postives = 178/331 (53.78%), Query Frame = 0

Query: 2   EAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDI 61
           E  EE   P++   C          P+SK   KKL+KQ+++E ++     + ++QK+K+ 
Sbjct: 23  EDQEESQKPRLGEGCE---------PISKRQMKKLIKQKQWEEQR-----ELRKQKRKE- 82

Query: 62  ERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVI 121
           +RKRK+ E                     R+ +M   S+  ++K  R          ++I
Sbjct: 83  KRKRKKLE---------------------RQCQMEPNSDGHDRKRVRRDVVHS-TLRLII 142

Query: 122 DLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDK-WI 181
           D  F HLM   +I  L +QI  CYA N R   P   +LT   G+++   + +   DK W+
Sbjct: 143 DCSFDHLMVLKDIKKLHKQIQRCYAENRRALHPVQFYLTSHGGQLK---KNMDENDKGWV 202

Query: 182 IEKERRSYIDALQE--QKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKA 241
             K+     +   E  +KE+L+YLT+DS  +L +LD  K Y++GGLVD N  KG+T K+A
Sbjct: 203 NWKDIHIKPEHYSELIKKEDLIYLTSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQA 262

Query: 242 QEQEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRS---- 301
            +  I  A+LP+G+++KM+S +VL VN V EI+L+YLETRDW+++FF ++PQRK +    
Sbjct: 263 SDYGINHAQLPLGNFVKMNSRKVLAVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTD 313

Query: 302 ---GADSNSDHPVDGEENGDGDNKNETKKTK 323
               + S+ +  V  EE G   + +E + ++
Sbjct: 323 KACESASHDNQSVRMEEGGSDSDSSEEEYSR 313

BLAST of Chy12G212230 vs. ExPASy Swiss-Prot
Match: Q3MHI8 (tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 8.0e-31
Identity = 105/321 (32.71%), Postives = 172/321 (53.58%), Query Frame = 0

Query: 27  PLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLASVTEEERAKLIES 86
           P+SK   KKL+KQ+++E ++     + ++QK+K+ +RKRK+ E +    +  + +     
Sbjct: 39  PISKRQMKKLMKQKQWEEQR-----ELRKQKRKE-KRKRKQLERQCQPESNSDGSDRKRI 98

Query: 87  RRNLRKERMGKRSEDREKKIERLSTARDYGQNIVIDLEFSHLMNPSEIHSLVQQIMYCYA 146
           RR++    +                       ++ID  F  LM   +I  L +QI  CYA
Sbjct: 99  RRDVVHSPL----------------------RLIIDCSFDSLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPCHLWLTGCNGEMETQLRRLPGFDK-WIIEKERRSYIDALQE--QKENLVYLT 206
            N R   P   +LT   G+++   + +   DK W+  K+     +   E  QKE+L+YLT
Sbjct: 159 ENRRALHPVQFYLTSHGGQLK---KNMDENDKGWVNWKDIHIKPEHYSEFIQKEDLIYLT 218

Query: 207 ADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQEIQTAKLPIGSYLKMSSSQVLT 266
           +DS  +L +LD  K Y++GGLVD N  KG+T K+A +  I  A+LP+G+++KM+S +VL 
Sbjct: 219 SDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDHGIDHAQLPLGNFVKMNSRKVLA 278

Query: 267 VNQVIEILLKYLETRDWKDSFFEVVPQRK---------RSGADSNSDHPVDGEENGDG-- 326
           VN V EI+L+YLETRDW+++FF ++PQRK          S +       V+G  N D   
Sbjct: 279 VNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDQACESCSHDKKFARVEGGLNSDSSE 328

Query: 327 -DNKNETKKTKCDEVSSSDHN 333
            +N++E   T  +E    +++
Sbjct: 339 EENRHELDSTHEEEKQDKENS 328

BLAST of Chy12G212230 vs. ExPASy Swiss-Prot
Match: Q66JJ4 (tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 5.2e-30
Identity = 113/348 (32.47%), Postives = 183/348 (52.59%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQ---AKEQK 60
           +EAV E     +DP       N  +   S++A      Q+   A +++ K Q   +K Q 
Sbjct: 3   LEAVTEPGKCSIDP-------NTKDLLASQHAGNNTPLQENSSAPRSECKAQDAMSKRQM 62

Query: 61  KKDIERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQ 120
           KK +  K+K+WE++     ++ + K    +R L ++   + + D   +       +    
Sbjct: 63  KKFL--KQKQWEDQRELRKQKRKEK--RQKRKLERQAQAEHNIDANSRKRFRHEVQPSAL 122

Query: 121 NIVIDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFD 180
            ++ID  F  LM   ++  L +QI  CYA N R   P  L+LT   G++++ +     +D
Sbjct: 123 RLIIDCSFDDLMALRDVKKLNKQIRRCYAENRRAVHPVQLYLTSHGGQLKSNMDE---YD 182

Query: 181 K-WI----IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKG 240
           K WI    I  +   Y D +  +KE+LVYLT+DS  VL +LD  K YI+GGLVD N  KG
Sbjct: 183 KGWINWKDIHIKPEHYKDLI--KKEDLVYLTSDSPEVLSELDETKAYIIGGLVDHNHHKG 242

Query: 241 ITMKKAQEQEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRK 300
           IT KKA E  I  A+LP+G+++KM++ +VL VN V EI+L +LE ++WK++FF V+PQRK
Sbjct: 243 ITYKKALELGISHAQLPLGNFVKMNTRKVLAVNHVFEIILAFLEKKEWKEAFFSVLPQRK 302

Query: 301 RS--------GADSNSDHPVDGEENGDGDNKNET----KKTKCDEVSS 329
            +          +  +    DGE++    + +E+       +C+E +S
Sbjct: 303 GAIPLTETGEQPECRASEQEDGEDSDSDSSIDESATIQPSVRCEEQNS 334

BLAST of Chy12G212230 vs. ExPASy TrEMBL
Match: A0A0A0LYJ3 (SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G173210 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 2.7e-175
Identity = 325/333 (97.60%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEAVEE HNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFSHLM P EIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQL+RLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKR G DSNS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDHNL 334
           DHPVDGEENGDGDNKNETKKTKCDEVSSSD+NL
Sbjct: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL 333

BLAST of Chy12G212230 vs. ExPASy TrEMBL
Match: A0A5D3CW30 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00280 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.6e-165
Identity = 307/327 (93.88%), Postives = 316/327 (96.64%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           ME VE  H+PK+DPPCS SACNPPEP LSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEEKLA VTEEERAKLIESRRNLRKERMGKRSEDREKKIERL+TA+D GQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFSHLM PSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEM +QL+RLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           QEIQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFF+VVPQRKR GADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVS 328
           DHPVDGEENGDGDNK ETKKTKCDEVS
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

BLAST of Chy12G212230 vs. ExPASy TrEMBL
Match: A0A1S3CL37 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502202 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.6e-165
Identity = 307/327 (93.88%), Postives = 316/327 (96.64%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           ME VE  H+PK+DPPCS SACNPPEP LSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEEKLA VTEEERAKLIESRRNLRKERMGKRSEDREKKIERL+TA+D GQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFSHLM PSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEM +QL+RLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           QEIQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFF+VVPQRKR GADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVS 328
           DHPVDGEENGDGDNK ETKKTKCDEVS
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

BLAST of Chy12G212230 vs. ExPASy TrEMBL
Match: A0A6J1I7W5 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111471663 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.6e-153
Identity = 286/331 (86.40%), Postives = 307/331 (92.75%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEA+EED + KMDPPCS S CNPPEPPLSKNARKKLLKQQRYEAKKA+KK Q KEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWE+ LASVTEEERAKLIESRR+LRKERMGKRSE++EKKIERL+ A+D GQ I+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFS LM PSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQL+RLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQ+QKENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFF+VVPQRKR  A+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDH 332
           DH VDGEENGD D++ E+KK KC EVSSS+H
Sbjct: 301 DHAVDGEENGDRDDEQESKKIKCGEVSSSNH 331

BLAST of Chy12G212230 vs. ExPASy TrEMBL
Match: A0A6J1EPG7 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434383 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 3.3e-152
Identity = 286/331 (86.40%), Postives = 306/331 (92.45%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEA EED + KMDPPCS S CNPPEPPLSKNARKKLLKQQRYEAKKA+KK Q KEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEE LASVTEEERAKLIESR++LRKERMGKRSE++EKKIERL+ A+D GQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFS LM PSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQL+RLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQ+ KENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQV+EILL+Y+ETRDWK SFF+VVPQRKR  A+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLRYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDH 332
           DH VDG+ENGD D++ ETKKTKCDEVSSS+H
Sbjct: 301 DHAVDGKENGDRDDEQETKKTKCDEVSSSNH 331

BLAST of Chy12G212230 vs. NCBI nr
Match: XP_004139517.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucumis sativus] >KGN65001.1 hypothetical protein Csa_022718 [Cucumis sativus])

HSP 1 Score: 624 bits (1610), Expect = 7.29e-225
Identity = 325/333 (97.60%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEAVEE HNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFSHLM P EIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQL+RLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKR G DSNS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDHNL 333
           DHPVDGEENGDGDNKNETKKTKCDEVSSSD+NL
Sbjct: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL 333

BLAST of Chy12G212230 vs. NCBI nr
Match: XP_008464275.1 (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase isoform X1 [Cucumis melo] >KAA0042332.1 tRNA (guanine(9)-N1)-methyltransferase isoform X1 [Cucumis melo var. makuwa] >TYK15458.1 tRNA (guanine(9)-N1)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 591 bits (1523), Expect = 1.18e-211
Identity = 307/327 (93.88%), Postives = 316/327 (96.64%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           ME VE  H+PK+DPPCS SACNPPEP LSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEEKLA VTEEERAKLIESRRNLRKERMGKRSEDREKKIERL+TA+D GQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFSHLM PSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEM +QL+RLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           QEIQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFF+VVPQRKR GADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVS 327
           DHPVDGEENGDGDNK ETKKTKCDEVS
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

BLAST of Chy12G212230 vs. NCBI nr
Match: XP_023520714.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 554 bits (1427), Expect = 5.76e-197
Identity = 289/331 (87.31%), Postives = 308/331 (93.05%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEA+EED + KMDPPCS S CNPPEPPLSKNARKKLLKQQRYEAKKA+KK Q KE KKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKELKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEE LASVTEEERAKLIESR++LRKERMGKRSE++EKKIERL+ A+D GQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFS LM PSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQL+RLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQ+QKENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFF+VVPQRKR  A+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDH 331
           DH VDGEENGD D++ ETKKTKCDEVSSS+H
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNH 331

BLAST of Chy12G212230 vs. NCBI nr
Match: XP_038893739.1 (tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida])

HSP 1 Score: 553 bits (1426), Expect = 9.13e-197
Identity = 295/337 (87.54%), Postives = 312/337 (92.58%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEAVEED + KMDPP S  A NPPEPPLSKNA+KKLLKQQRYEAKKA+KKLQAKEQKKKD
Sbjct: 1   MEAVEEDQSAKMDPPPSPFAFNPPEPPLSKNAKKKLLKQQRYEAKKAEKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWE+ LASVTEEER+KLIESRR+LRKERMGKRSE+REKKIERL+ A+D GQNIV
Sbjct: 61  IERKRKEWEDTLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNRAKDCGQNIV 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFSHLM PSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQL+RLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQ+QKENLVYLTAD+ETVLDDLD KKIYI+GGLVDRNRWKGITMKKA E
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGITMKKAHE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFF+VVPQRKR GADSNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCGADSNS 300

Query: 301 DHPVDGEENGDGD----NKNETKKTKCDEVSSSDHNL 333
           DHPVD EENGDGD    +++E KKTKCDEVSSSD  L
Sbjct: 301 DHPVDEEENGDGDGDGDDEHEMKKTKCDEVSSSDDTL 337

BLAST of Chy12G212230 vs. NCBI nr
Match: KAG6583717.1 (hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 553 bits (1425), Expect = 1.16e-196
Identity = 289/331 (87.31%), Postives = 308/331 (93.05%), Query Frame = 0

Query: 1   MEAVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEA EED + KMDPPCS S CNPPEPPLSKNARKKLLKQQRYEAKKA+KK Q KEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEE LASVTEEERAKLIESR++LRKERMGKRSE++EKKIERL+ A+D GQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWI 180
           IDLEFS LM PSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQL+RLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IE+E RSYIDALQ+QKENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFF+VVPQRKR  A+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDH 331
           DH VDGEENGD D++ ETKKTKCDEVSSS+H
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNH 331

BLAST of Chy12G212230 vs. TAIR 10
Match: AT5G47680.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 395.2 bits (1014), Expect = 5.2e-110
Identity = 220/331 (66.47%), Postives = 263/331 (79.46%), Query Frame = 0

Query: 3   AVEEDHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIE 62
           A   D N   D P S  A     PPLSKNA+KK LKQQRYEAKKA+KK Q KE K+K+ E
Sbjct: 4   ATGNDDNGCFDKPESEPAPVNVSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEHKRKEGE 63

Query: 63  RKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVID 122
           RK KEWEE LA+ TEEER KLIESR++LRKERM KRSE++EKKIERL+ A++ GQ IV+D
Sbjct: 64  RKLKEWEETLANATEEERLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEIGQKIVVD 123

Query: 123 LEFSHLMNPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLRRLPGFDKWIIE 182
           ++F+HLM+ SEI SLVQQIMYCYAVNGR  SPCHLWLTG  G+M T+L +LPGF+KW IE
Sbjct: 124 VDFAHLMSESEISSLVQQIMYCYAVNGRSTSPCHLWLTGVQGKMSTELDKLPGFEKWFIE 183

Query: 183 KERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQE 242
           KE R YI+A+ +QK+NLVYLTADSETVLDDLD K IYI+GGLVDRNR+KGITM KAQEQ 
Sbjct: 184 KESRCYIEAMADQKDNLVYLTADSETVLDDLDPKHIYIIGGLVDRNRFKGITMTKAQEQG 243

Query: 243 IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRSGADSNSDH 302
           I+TAKLPIG Y+KMSSSQVLTVNQV+EIL+K+LETRDWK +FF V+PQRKR+G D     
Sbjct: 244 IKTAKLPIGEYMKMSSSQVLTVNQVLEILVKFLETRDWKTAFFTVIPQRKRTGLD----- 303

Query: 303 PVDGEENGDGDNKNETKKTKCDEVSSSDHNL 334
           PVD  +      +++ K T+ +E S+  H+L
Sbjct: 304 PVDCSKLEHISEEHQEKDTEANE-SADKHDL 328