Chy12G210290 (gene) Cucumber (hystrix) v1

Overview
NameChy12G210290
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionberberine bridge enzyme-like 18
LocationchrH12: 5203226 .. 5208507 (-)
RNA-Seq ExpressionChy12G210290
SyntenyChy12G210290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAGTGAAGGAGATGGGAAAGTAAAGCCAGTAGCTATATTCTACACATCATTTCTTGGGAAGGCAAATAAGGCAGTGAAAATCCTAAAAGAAAAATTTCCTCAATTGGGTTTAAAAAAGGAAGAGTGCAAAGAAACAAGTTGGGTTGAATCAGTTGTTATTGCTGCCAATGATTTTAAAGATGGAGAGCCCGTTGAAGCCTTGCTCAATAGATCAGCATTGATTCCACCTATCACAAGCAAAAAAGTAAAAATAAAATCTGATTATGTAAAGGAACCCATGCCCAAAGTTGCAATTGAAGGCATATGGAATAGAGTAAATAACTCCCAAGACATAGGAGGAATAAACGTTCTATTTGTTCCTTATGGAGGGAGAATGAGTGAGATTTCTGAGTCCGAAATTCCTTTCTCACATAGAGCTGGAAATTTGTACAAAATTGCATACCTTACTGGATGGGAAGACCCAAGTATTGATGTAGAGTCAAGACATTTAAATTGGATACGAGAAATTTACAGTTACATGGCTCCTTTTGTTTCCAAATCACCTAGAAGTGCATATGTCAATTACAGAGATCTTGATATTGGATCCAACTCCGACAAGTACGGAAACATCGTGACAAACCATGACCAAGCTAGCAGTTGGGGTTTGAAATACTATGGTAACAATTTTAATAGGTTGGTACAAATTAAAACTAAGGTGGATCCCTATAATTTCTTTAGGCATGAGCAAAGCATTCCAACCGCTCTTTCTCAAGAAGATAATTTAGTAAAATTAGTTTAGTAGTTTGATATTTCTGTTATGTGACTGTAATTGAATTATGCCCCAACCTATTATTATAGTGTGTGCAGAATAATGGGTCGCTTGTGTCCTTTATGCATCAATCTATCTTTTTTTTTTTTTTCTTTTTTGATGGTTAAGTTAACACTATAATTCATCAGGTTTGAAAAATAATTCTATGGTTGACGCTCATCCTGTTGCCACGGTAATGGTATAGTTTGCTTTCCAAGCATTTTGTAACATTTTAATATAAAATCGATTTGGTATTTTAATAATTTATTTTTATTAATTTTACATTAATTTTTTTTAGAATTTGATTGAATTTAAATCGAATCTGATTCCAATAACAAAAAAAAAAAATACGCATACAATGCTTCTCTGACGTCGTTCTCCAGTTGGCGTAGACCCCATCGGGCAAAAGCTTTAGCCTATCATGACGCAACATTGTCAATGCTCTTCCCGACGTGGCATTTTGCATTAGGATAGACACATCCGATGTGTTTTGTAAATTTTGTTGATGCATATGTGCGTCGGGCGAAGCACTACTTCTTGTAGTGGATCAATATGTAAGAGTTCAAGAACATGACTTGTAGTACATTGCCCCCACAGACTTGTGTGATGCTTTAACTTGTTTTTCAACAGGACATTCTACACATAAATTTTCTGAAGCATTCTTCAATAAAGGTATTCTTAAATTTGCTTCTGCCCTGATAGTCTTGCAAATGGTTGAGATACTTATGTGTCCAAGACGTTTATGCCACAAATCTGCTTCATCGGACTTCGAAAAATTACACATATAATTCTTTAGATAATTGTCCCAACGATAATAATTATCTCATAATCGTGTGCTCGTCATTACAATACTTTTGTCTTGATTCAGAACTTTATATGTGTGTCTTTAGTGAAAGTGACAGTGAATCCTTGATCACACAGTTGACTGACACTAATCAAATTTGCTGATAAACCCTTTACAAGCTTGACATTATAAACAAGAACAGGTAATCCATATCTACTTATGTTTCCTTTCCCTATAACTTTTCCTTTTACTCCATCACCAAAAGTCACATGACCCACGTTGCATTCCTTAAATTCAGTTAAAAACAAGAAGTTTCCAGTCATATGTCTAGAGCATCTACTGTCAAAATACTAATCATCTAAATTAATATATGTATAAATAGATGTAAACGCCACATTACATAGGTTGTGTTTTATTTCGTTCTTCGCTCTCTACTCAATTTTTGTAGAGAGACGTGGCTAATTTAGTAAGGTTTTTCTGCTACTTTTATTATTTAATCTTCTATGACTAAAACAACTATATCCCTGTAGCTTGTAACAAAATGGTCGAATATTACCAAATTTTCACAGAAATGACATGTCCACCTTCTTGGAGAGCTTGGGCACACCCTGTGATGGTTTGTTTTGACATACAATACCTCAGAGGGTTCTACACTCCCATTCTTGCTATAAGATGCTCTAACAAATACTATTGATGACCCTCCAGTACACAAACCTTTTGCTGAAAAAGCAAGGCATTTTTTATCTTTCTTAATCTTCCCTTCACTAAAAAGATTATCCAAACTTTGAGTTCTTGAGGTGAGTGACTTTACCGATTTAGACAAGGAGTCAGATTCACTCCGAACATCTCTCGGTTCCATTTTAAAAGATTATCCAAACTGTTTGTACTCTCTCCTTCTGAAGTTTCAAAGCTTCCTGATCTTCTTCCCATTTAGACAACAAGTCCTCACTAGGTCAGCTTTTCTATCATATTCACTAATTAATTTCAGATCAGATACACTCTTAGAAACTTCTAATTCCTTTTAAGTTGTACAACTTATAAGAGCTCTGTCTTATTCTTCTCTATCACTATCAGATGATGATTCCTCATCTGATAGAGTAATTGTGAGGCTCTTCTTTTTACTTTTTATAAAGGCAAGACACTCAGCTTGAAAATGCCCAAAACCTTCACACTTTCATGACACCTGAAACTTCTATTATTTCTGTTAGATGTCAAATTTCTATCACGTTTGTCAATATCTTTATGCTTATGAGCCACATACGAACTAGAAGAGCTTGATCTTGAGTTACTTGAATTACTGAACTCTCTGTTATACTTAGTTTCGTAATTACCGAATCTTTTATGAACCTGGCTTCTAATTAACTTTTGAAACCTACTTTGTTAGTAAGGTTACAGACTCGCCTAAATTTTCATCATGAGTACGCTCTTTGCTCAATTGAGGAGTGTCCTCATTTACAACTTGAAGAGCAATTCCACTTTTCTTCTTAGTTTCTCCATCTTCGAGTTTTAGCTCAAAAGTTCTCAGGGACCCAAACAGCTCATCTAATTTTGAACAAGCTTAGTATCTGAGATCTTTTCTCATAAAGCAAATGATTCATTTCCTAAGTCAAGAATTCAAACCTTAAACTCAGTTATGGATTCATCTTCAGCCATCTTGAGAGCTTCAAATTTTGATCAAGATCTGAAACCGTGACATTTTCACTCGAAAGGTGCCTTCATAAGCCACCTTCAGAATACTCCAAGTCTCCTTTATAGACCTATAAGTATTGATCAATTTGAACACATTTTTATCTACTCCATTAAAAATTGGATTCAAAGCACAAGAATTTAAAAAAAAAAATGTTTCATCTTCTACACTGGACCAGGAGATTTCTAGCTTTTGATAGACTTTACTAGTAGTATCTGTAACAATAGGGGTGTTCCCACCTATAACAACATTCCAAGATTTGCTATCAATTGATTTCAAGAAAGCAATCATTCTAGCTTTTCAGTAGGCATAGTTAGATCCATCCAGAATTGGAGGTTGTGTAGTAAAGCTTCCTTCATGCACAGGTACTATAAGACACACCAATTATGTAACTCGCTTTGATACCAAATGAGATAAAACAATCAACACTCATGCTTCAAGCACACACCTTAGTTAATAACTGAAGAAACAACTTAAAGAACAATATTGTAATGGGTCCCTAAGACATAACAAATGAATAAGTAGACACAACAAATTAAACACCATACATCTAAGGAGTTCTTGACCCATGATAAGAGATTTCGTGTTGCCTTGTGATATAATTCATGATTACAGACCTTATGACTATTAGTAAAAGGGATAGTTGCAAATTTAGCAATTAAATTCAAAATAATTAGGTATATAGCAACATTTTAAACAATTTGTAAATATAGCAAAATTTGTCAAATTCTATCAATGATAAAAGTTTATCATTGATAGACCATGTTGCAAATATTGATTTATCACTGATAGATCATACGAGTCTATCATCAATACAAGTCTATCAACAATAGTTTTGCGATATTTGCAATTTTTTTTAAATGTTGCTACATTCTTAATTATTATTCCTAAAATGGCCATCAATTACAATTTTCCTAGTAAAAAATTCACAACACATTTTCAAACTTACTTTTGTACTTAGTGACACTTTCTTACTGACACATGAAACTAACAACTAATGTTCGTCATAAATGTCACAAAGGAAGACTTCCCCATTAATCAGTGATCACTTTAAGCAATTAATCGAGTTGGACTTGATCTTCAACAATCTCGAATCTCAGATTCTTGGCTAGAAAAACTTTTTTTAACACACTTGACTCACCAAGTAGTACACGCACTCTTTCAGCTTTATCTTTCAATCACAATCTGCACAATGACATATACCCTTACTTTTTTTTTTGCACACCCCAAAACACCCACGAACCAGAAAGATAAACATACTCTAGAGAACCAACCAACCCACAAAGTTAAGAACGAATTTCAACAACCAGAAAACTCACAACAACGCAGCCAAAACAATAATATTTTCTCAAATGTTATCGAGACCACCAACACAACATATTTATTATTCATAGAATATAAAATTTGACTATATTTTGTACTTATATTTCGATCATTTTATATTTCAAAATGCCTCTTTATTTGACCTGTCAGGAGCATTTATTACTTTCTTCTCGAATTTTTTTTAATGGATACTATATATCTTTACTCTGTTTGACCTTCTTTTCTGGTTTAATTTATTTCTTAAAACTAATTTGAAATGAAATAATTTATTTGTGATTGGATTAAGATATACACTCTCTCATTAATTACAGTATTTTGAGATCAAAGTTTATTCCTGTTGATATATAGGTAGAAAGATTTGAAGTAAAGGTAATAGCTTTATTCTTCTCTTTATTTCTTGGAAAGGCAGAGGAATTAGTAGCAATCCTAAAAGAAACATATTCAAATTGTGTTTATCAAAAGAAGTGTATAGAAACAAGTTGGATTGAATCAACATTTATTGTTGCCAATTGAAGAACTACCCATTGAATCTTTGCTTAATAATTCAGCATTTGATTCCAGCTATTAGTAGGCAAGAGAAGCAAAATAAAACTTGATAATGTAAAGGAACCCATGACTGAAGTTGCAACTGAAGGCATGTGGAATAGATTAAAATTTCAAGATATAGAAGGAGAAAACATTATAATGGTTTCTTAA

mRNA sequence

ATGACAAGTGAAGGAGATGGGAAAGTAAAGCCAGTAGCTATATTCTACACATCATTTCTTGGGAAGGCAAATAAGGCAGTGAAAATCCTAAAAGAAAAATTTCCTCAATTGGGTTTAAAAAAGGAAGAGTGCAAAGAAACAAGTTGGGTTGAATCAGTTGTTATTGCTGCCAATGATTTTAAAGATGGAGAGCCCGTTGAAGCCTTGCTCAATAGATCAGCATTGATTCCACCTATCACAAGCAAAAAAGTAAAAATAAAATCTGATTATGTAAAGGAACCCATGCCCAAAGTTGCAATTGAAGGCATATGGAATAGAGTAAATAACTCCCAAGACATAGGAGGAATAAACGTTCTATTTGTTCCTTATGGAGGGAGAATGAGTGAGATTTCTGAGTCCGAAATTCCTTTCTCACATAGAGCTGGAAATTTGTACAAAATTGCATACCTTACTGGATGGGAAGACCCAAGTATTGATGTAGAGTCAAGACATTTAAATTGGATACGAGAAATTTACAGTTACATGGCTCCTTTTGTTTCCAAATCACCTAGAAGTGCATATGTCAATTACAGAGATCTTGATATTGGATCCAACTCCGACAAGTACGGAAACATCGTGACAAACCATGACCAAGCTAGCAGTTGGGGTTTGAAATACTATGGTAACAATTTTAATAGGTTGGTACAAATTAAAACTAAGGTGGATCCCTATAATTTCTTTAGGCATGAGCAAAGCATTCCAACCGCTCTTTCTCAAGAAGATAATTTACTATTAGTAGGCAAGAGAAGCAAAATAAAACTTGATAATGTAAAGGAACCCATGACTGAAGTTGCAACTGAAGGCATGTGGAATAGATTAAAATTTCAAGATATAGAAGGAGAAAACATTATAATGGTTTCTTAA

Coding sequence (CDS)

ATGACAAGTGAAGGAGATGGGAAAGTAAAGCCAGTAGCTATATTCTACACATCATTTCTTGGGAAGGCAAATAAGGCAGTGAAAATCCTAAAAGAAAAATTTCCTCAATTGGGTTTAAAAAAGGAAGAGTGCAAAGAAACAAGTTGGGTTGAATCAGTTGTTATTGCTGCCAATGATTTTAAAGATGGAGAGCCCGTTGAAGCCTTGCTCAATAGATCAGCATTGATTCCACCTATCACAAGCAAAAAAGTAAAAATAAAATCTGATTATGTAAAGGAACCCATGCCCAAAGTTGCAATTGAAGGCATATGGAATAGAGTAAATAACTCCCAAGACATAGGAGGAATAAACGTTCTATTTGTTCCTTATGGAGGGAGAATGAGTGAGATTTCTGAGTCCGAAATTCCTTTCTCACATAGAGCTGGAAATTTGTACAAAATTGCATACCTTACTGGATGGGAAGACCCAAGTATTGATGTAGAGTCAAGACATTTAAATTGGATACGAGAAATTTACAGTTACATGGCTCCTTTTGTTTCCAAATCACCTAGAAGTGCATATGTCAATTACAGAGATCTTGATATTGGATCCAACTCCGACAAGTACGGAAACATCGTGACAAACCATGACCAAGCTAGCAGTTGGGGTTTGAAATACTATGGTAACAATTTTAATAGGTTGGTACAAATTAAAACTAAGGTGGATCCCTATAATTTCTTTAGGCATGAGCAAAGCATTCCAACCGCTCTTTCTCAAGAAGATAATTTACTATTAGTAGGCAAGAGAAGCAAAATAAAACTTGATAATGTAAAGGAACCCATGACTGAAGTTGCAACTGAAGGCATGTGGAATAGATTAAAATTTCAAGATATAGAAGGAGAAAACATTATAATGGTTTCTTAA

Protein sequence

MTSEGDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPTALSQEDNLLLVGKRSKIKLDNVKEPMTEVATEGMWNRLKFQDIEGENIIMVS*
Homology
BLAST of Chy12G210290 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.9e-56
Identity = 112/232 (48.28%), Postives = 150/232 (64.66%), Query Frame = 0

Query: 19  FLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGEPVEALLNRSALIPP 78
           FLG AN+ +++++  FPQLGL K++C ETSW++SV+  A  F    P EALL+  +L   
Sbjct: 319 FLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAG-FPSTAPSEALLDGKSLF-- 378

Query: 79  ITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEIPFS 138
                 K KSDYV+EP+P   +EG+W ++           ++ PYGG M++I E+E PF 
Sbjct: 379 --KNYFKAKSDYVEEPIPVEGLEGLWEKLLEED---SPLTIWNPYGGMMAKIPETETPFP 438

Query: 139 HRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSN 198
           HR+G L+KI +LT W+D     E++H+ W+RE+YSYM  +VSKSPRSAYVNYRDLD+G N
Sbjct: 439 HRSGTLFKIQWLTLWQDGKTS-EAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN 498

Query: 199 SDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPTAL 251
                        A  WG +Y+  NF RLV+IK K DP NFFRHEQSIPT L
Sbjct: 499 G--------KGSDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of Chy12G210290 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.9e-56
Identity = 111/236 (47.03%), Postives = 149/236 (63.14%), Query Frame = 0

Query: 15  FYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGEPVEALLNRSA 74
           +   FLG +N+ ++++++ FP+LGL K++C E SW++SV+  A  F +    EALL   +
Sbjct: 313 YQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAG-FPNSAAPEALLAGKS 372

Query: 75  LIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESE 134
           L         K KSD+VKEP+P   +EG+W R            ++ PYGG MS ISESE
Sbjct: 373 LF----KNHFKAKSDFVKEPIPVEGLEGLWERFLEED---SPLTIWNPYGGMMSRISESE 432

Query: 135 IPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLD 194
           IPF HR G L+KI +L+ W+D  +  E RH+ WIRE+YSYM  +VSK+PR AYVNYRDLD
Sbjct: 433 IPFPHRNGTLFKIQWLSTWQDGKVS-EERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLD 492

Query: 195 IGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPTAL 251
           +G+N             A  WG KYY  NF RLV+IK + DP NFFRHEQS+PT +
Sbjct: 493 LGTNE--------GETDAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of Chy12G210290 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.5e-55
Identity = 110/249 (44.18%), Postives = 158/249 (63.45%), Query Frame = 0

Query: 2   TSEGDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFK 61
           T E   K    A+F   ++G  N  + +++EKFP+LGL+KE C+E SW+ESV+  A DF 
Sbjct: 289 TLERSNKNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFA-DFP 348

Query: 62  DGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFV 121
            GE +  L NR       TS   K K D+V+EP+P+ AI+ IW R+   +   G  ++  
Sbjct: 349 KGESLGVLTNRER-----TSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLG-KIILT 408

Query: 122 PYGGRMSEISESEIPFSHRAGNLYKIAYLTGW---EDPSIDVESRHLNWIREIYSYMAPF 181
           P+GG+MSE++E E PF HR GNLY+I Y+  W   ED +     ++L W+  +Y +M P+
Sbjct: 409 PFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPY 468

Query: 182 VSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYN 241
           VSKSPR AYVN++D+D+G      G   T +++  SWG+KY+ NNF RLV++KT+VDP +
Sbjct: 469 VSKSPRGAYVNFKDMDLGM---YLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTD 527

Query: 242 FFRHEQSIP 248
           FF  EQSIP
Sbjct: 529 FFCDEQSIP 527

BLAST of Chy12G210290 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 7.2e-55
Identity = 113/243 (46.50%), Postives = 151/243 (62.14%), Query Frame = 0

Query: 6   DGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGEP 65
           DG       +   FLG+    +K++++ FP+LGL +++C E SW+E+ +     F  G P
Sbjct: 307 DGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGG-FPTGSP 366

Query: 66  VEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVL-FVPYG 125
           +E LL    L  P+     K  SD+VKEP+P + ++GI+ R+      G    L + PYG
Sbjct: 367 IEILLQ---LKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIE----GNTTFLNWTPYG 426

Query: 126 GRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPR 185
           G MS+I ES IPF HR G L+KI Y   W + +    SR +NWI+EIY+YMAP+VS +PR
Sbjct: 427 GMMSKIPESAIPFPHRNGTLFKILYYANWLE-NDKTSSRKINWIKEIYNYMAPYVSSNPR 486

Query: 186 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 245
            AYVNYRDLD G N +   N   N  +A  WG KY+  NF+RLV+IKTKVDP NFFRHEQ
Sbjct: 487 QAYVNYRDLDFGQNKN---NAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQ 537

Query: 246 SIP 248
           SIP
Sbjct: 547 SIP 537

BLAST of Chy12G210290 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-54
Identity = 114/248 (45.97%), Postives = 155/248 (62.50%), Query Frame = 0

Query: 3   SEGDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVI---AAND 62
           ++G  K      F + FLG  +  V ++ + FP+LG+KK +C++ SW+++++      N 
Sbjct: 300 NQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNY 359

Query: 63  FKDGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVL 122
             D    E LL+RSA      +   KIK DYVK+P+P+     I  ++   +DIG     
Sbjct: 360 DTDNFNKEILLDRSA----GQNGAFKIKLDYVKKPIPESVFVQILEKL-YEEDIGAGMYA 419

Query: 123 FVPYGGRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFV 182
             PYGG M EISES IPF HRAG LY++ Y+  WE    +   +HLNWIR IY++M P+V
Sbjct: 420 LYPYGGIMDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYV 479

Query: 183 SKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNF 242
           SK+PR AY+NYRDLDIG N  K  N   N+ QA  WG KY+G NF+RLV++KT VDP NF
Sbjct: 480 SKNPRLAYLNYRDLDIGINDPKNPN---NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNF 537

Query: 243 FRHEQSIP 248
           FR+EQSIP
Sbjct: 540 FRNEQSIP 537

BLAST of Chy12G210290 vs. ExPASy TrEMBL
Match: A0A0A0LYM2 (BBE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G540370 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.7e-139
Identity = 247/257 (96.11%), Postives = 251/257 (97.67%), Query Frame = 0

Query: 1   MTSEGDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDF 60
           MTSE DGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKE SWVESVVIAANDF
Sbjct: 10  MTSEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDF 69

Query: 61  KDGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF 120
             GEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF
Sbjct: 70  TVGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF 129

Query: 121 VPYGGRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVS 180
           VPYGGRMSEISESEI FSHRAGNL+KIAYLTGWEDPS+DVE+RHLNWIREIYSYMAPFVS
Sbjct: 130 VPYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVS 189

Query: 181 KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF 240
           KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF
Sbjct: 190 KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF 249

Query: 241 RHEQSIPTALSQEDNLL 258
           RHEQSIP ALSQEDNL+
Sbjct: 250 RHEQSIPIALSQEDNLV 266

BLAST of Chy12G210290 vs. ExPASy TrEMBL
Match: A0A6J1KJP1 (berberine bridge enzyme-like 27 OS=Cucurbita maxima OX=3661 GN=LOC111495223 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 2.3e-88
Identity = 160/244 (65.57%), Postives = 197/244 (80.74%), Query Frame = 0

Query: 5   GDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGE 64
           G GK  P+AIF++ FLGKA++ V IL   FP+LGL KE+C ETSW+E+ +IAAN F+ GE
Sbjct: 293 GGGKTNPLAIFFSLFLGKADELVTILNNPFPELGLSKEDCIETSWIEAALIAANGFQTGE 352

Query: 65  PVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYG 124
            +EALLNRSALIPPI S + K KSDYVKEPM + AIEG+WNR + SQ+I   +++F+PYG
Sbjct: 353 VLEALLNRSALIPPINSGRSKTKSDYVKEPMTRTAIEGMWNR-SKSQEIERASLVFIPYG 412

Query: 125 GRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPR 184
           G+MSEISESEIPF HRAGN Y+I Y+ GW++  +  E RHLNWIRE+YSYM PFVSKSPR
Sbjct: 413 GKMSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPR 472

Query: 185 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 244
           + YVN RDLDIGSN D YG   T++ +A  WGLKY+GNNF+RLV +KT+VDPY+FFRHEQ
Sbjct: 473 AVYVNCRDLDIGSN-DMYGR--TSYKRARVWGLKYFGNNFDRLVHVKTEVDPYDFFRHEQ 532

Query: 245 SIPT 249
           SIPT
Sbjct: 533 SIPT 532

BLAST of Chy12G210290 vs. ExPASy TrEMBL
Match: A0A6J1EMV2 (berberine bridge enzyme-like 27 OS=Cucurbita moschata OX=3662 GN=LOC111434009 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 2.3e-88
Identity = 161/244 (65.98%), Postives = 197/244 (80.74%), Query Frame = 0

Query: 5   GDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGE 64
           G GK  P+AIF++ FLGKA++ V IL   FP+LGL K +C ETSW+E+ +IAAN F+ GE
Sbjct: 293 GGGKTNPLAIFFSLFLGKADELVTILNNAFPELGLPKGDCIETSWIEAALIAANGFQTGE 352

Query: 65  PVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYG 124
            +EALLNRSALIPPI S K K KSDYVKEPM + AIEG+WNR + SQ+I   +++F+PYG
Sbjct: 353 VLEALLNRSALIPPINSGKSKTKSDYVKEPMTRTAIEGMWNR-SKSQEIERASLVFIPYG 412

Query: 125 GRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPR 184
           G+MSEISESEIPF HRAGN Y+I Y+ GW++  +  E RHLNWIRE+YSYMAPFVSKSPR
Sbjct: 413 GKMSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMAPFVSKSPR 472

Query: 185 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 244
           +AYVN RDLDIGSN D YG   T++ +A  WGLKY+GNNF+RL  +KT+VDPY+FFRHEQ
Sbjct: 473 AAYVNCRDLDIGSN-DMYGR--TSYQRARVWGLKYFGNNFDRLAHVKTEVDPYDFFRHEQ 532

Query: 245 SIPT 249
           SIPT
Sbjct: 533 SIPT 532

BLAST of Chy12G210290 vs. ExPASy TrEMBL
Match: A0A0A0LY37 (BBE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G539350 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 5.1e-88
Identity = 161/254 (63.39%), Postives = 202/254 (79.53%), Query Frame = 0

Query: 1   MTSEGDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDF 60
           ++++GD K+ P+A+F++ FLGKA++ + IL + FPQLGL KEECKETSW+ESVV   N  
Sbjct: 11  ISAQGD-KINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGL 70

Query: 61  K-DGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVL 120
           + + +P+E LLNR+    P+ +  +K+KSDYVKEP+PK  IE IW R+  SQDI G N++
Sbjct: 71  QIEDQPLEVLLNRT----PLATGNIKMKSDYVKEPIPKATIEEIWQRL-ESQDIEGANLV 130

Query: 121 FVPYGGRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFV 180
           FVPYGGRMS+IS+SEIPFSHRAGNLYKI YLTGW +P ++ E  HLNWIR+IY YM PFV
Sbjct: 131 FVPYGGRMSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFV 190

Query: 181 SKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNF 240
           SKSPR+AYVNYRDLDIGSNS KYG   T++ +A  WGLKY+GNNFNR+V +K KVDPYNF
Sbjct: 191 SKSPRAAYVNYRDLDIGSNS-KYGK--TSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNF 250

Query: 241 FRHEQSIPTALSQE 254
           FRHEQSIP  L  +
Sbjct: 251 FRHEQSIPVLLKHK 255

BLAST of Chy12G210290 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 1.2e-84
Identity = 158/247 (63.97%), Postives = 194/247 (78.54%), Query Frame = 0

Query: 2   TSEGDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFK 61
           T++G  K  P A F++ FLGKA++   IL   FP+LGL KE C E+SW+ES +++A  FK
Sbjct: 301 TAQGGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAG-FK 360

Query: 62  DGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFV 121
            G+ +EALLNR+    P+T+  +KIKSDYVKEP+ +  IEGIW R+  S+D+G   +LFV
Sbjct: 361 IGDSLEALLNRT----PLTNGSIKIKSDYVKEPISEATIEGIWERL-KSRDVGARQLLFV 420

Query: 122 PYGGRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSK 181
           PYGGRMS+ISESEIPFSHRAGNLYKI Y+  W+  ++DVE RHL WIRE+YSYM PFVSK
Sbjct: 421 PYGGRMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSK 480

Query: 182 SPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFR 241
           SPRSAYVNYRDLDIGSN +KYG   T+  +AS WG KY+GNNFNRLV +KTKVDPY+FFR
Sbjct: 481 SPRSAYVNYRDLDIGSN-NKYGK--TSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFR 538

Query: 242 HEQSIPT 249
           HEQSIPT
Sbjct: 541 HEQSIPT 538

BLAST of Chy12G210290 vs. NCBI nr
Match: KAE8653320.1 (hypothetical protein Csa_023331 [Cucumis sativus])

HSP 1 Score: 499 bits (1285), Expect = 7.10e-173
Identity = 247/257 (96.11%), Postives = 251/257 (97.67%), Query Frame = 0

Query: 1   MTSEGDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDF 60
           MTSE DGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKE SWVESVVIAANDF
Sbjct: 275 MTSEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDF 334

Query: 61  KDGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF 120
             GEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF
Sbjct: 335 TVGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF 394

Query: 121 VPYGGRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVS 180
           VPYGGRMSEISESEI FSHRAGNL+KIAYLTGWEDPS+DVE+RHLNWIREIYSYMAPFVS
Sbjct: 395 VPYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVS 454

Query: 181 KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF 240
           KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF
Sbjct: 455 KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF 514

Query: 241 RHEQSIPTALSQEDNLL 257
           RHEQSIP ALSQEDNL+
Sbjct: 515 RHEQSIPIALSQEDNLV 531

BLAST of Chy12G210290 vs. NCBI nr
Match: XP_004135739.1 (berberine bridge enzyme-like 28 [Cucumis sativus])

HSP 1 Score: 499 bits (1285), Expect = 1.58e-172
Identity = 247/257 (96.11%), Postives = 251/257 (97.67%), Query Frame = 0

Query: 1   MTSEGDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDF 60
           MTSE DGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKE SWVESVVIAANDF
Sbjct: 299 MTSEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDF 358

Query: 61  KDGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF 120
             GEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF
Sbjct: 359 TVGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLF 418

Query: 121 VPYGGRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVS 180
           VPYGGRMSEISESEI FSHRAGNL+KIAYLTGWEDPS+DVE+RHLNWIREIYSYMAPFVS
Sbjct: 419 VPYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVS 478

Query: 181 KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF 240
           KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF
Sbjct: 479 KSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFF 538

Query: 241 RHEQSIPTALSQEDNLL 257
           RHEQSIP ALSQEDNL+
Sbjct: 539 RHEQSIPIALSQEDNLV 555

BLAST of Chy12G210290 vs. NCBI nr
Match: XP_023520064.1 (berberine bridge enzyme-like 27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 331 bits (848), Expect = 2.03e-106
Identity = 159/244 (65.16%), Postives = 199/244 (81.56%), Query Frame = 0

Query: 5   GDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGE 64
           G GK  P+AIF++ FLGKA++ V IL   FP+LGL KE C ETSW+E+ +IAAN F+ GE
Sbjct: 293 GGGKTNPLAIFFSLFLGKADELVTILNNAFPELGLSKENCIETSWIEAALIAANGFQTGE 352

Query: 65  PVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYG 124
            +EALLNRSALIPPI S + K KSDYVK+PM + AIEG+WNR + SQ+I   +++F+PYG
Sbjct: 353 VLEALLNRSALIPPINSGRSKTKSDYVKQPMTRTAIEGMWNR-SKSQEIERASLVFIPYG 412

Query: 125 GRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPR 184
           G+MSEIS+SEIPF HRAGN Y+I Y+ GW++  ++ E RHLNW+RE+YSYMAPFVS SPR
Sbjct: 413 GKMSEISDSEIPFPHRAGNRYEIDYIVGWDEQGVEAEKRHLNWMRELYSYMAPFVSNSPR 472

Query: 185 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 244
           +AYVN RDLDIGSN D YG   T++++A  WGLKY+GNNF+RLV +KTKVDPY+FFRHEQ
Sbjct: 473 AAYVNCRDLDIGSN-DMYGR--TSYERARVWGLKYFGNNFDRLVHVKTKVDPYDFFRHEQ 532

Query: 245 SIPT 248
           SIPT
Sbjct: 533 SIPT 532

BLAST of Chy12G210290 vs. NCBI nr
Match: XP_022927075.1 (berberine bridge enzyme-like 27 [Cucurbita moschata])

HSP 1 Score: 330 bits (846), Expect = 3.83e-106
Identity = 161/244 (65.98%), Postives = 197/244 (80.74%), Query Frame = 0

Query: 5   GDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGE 64
           G GK  P+AIF++ FLGKA++ V IL   FP+LGL K +C ETSW+E+ +IAAN F+ GE
Sbjct: 293 GGGKTNPLAIFFSLFLGKADELVTILNNAFPELGLPKGDCIETSWIEAALIAANGFQTGE 352

Query: 65  PVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYG 124
            +EALLNRSALIPPI S K K KSDYVKEPM + AIEG+WNR + SQ+I   +++F+PYG
Sbjct: 353 VLEALLNRSALIPPINSGKSKTKSDYVKEPMTRTAIEGMWNR-SKSQEIERASLVFIPYG 412

Query: 125 GRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPR 184
           G+MSEISESEIPF HRAGN Y+I Y+ GW++  +  E RHLNWIRE+YSYMAPFVSKSPR
Sbjct: 413 GKMSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMAPFVSKSPR 472

Query: 185 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 244
           +AYVN RDLDIGSN D YG   T++ +A  WGLKY+GNNF+RL  +KT+VDPY+FFRHEQ
Sbjct: 473 AAYVNCRDLDIGSN-DMYGR--TSYQRARVWGLKYFGNNFDRLAHVKTEVDPYDFFRHEQ 532

Query: 245 SIPT 248
           SIPT
Sbjct: 533 SIPT 532

BLAST of Chy12G210290 vs. NCBI nr
Match: XP_023000935.1 (berberine bridge enzyme-like 27 [Cucurbita maxima])

HSP 1 Score: 330 bits (846), Expect = 3.83e-106
Identity = 160/244 (65.57%), Postives = 197/244 (80.74%), Query Frame = 0

Query: 5   GDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGE 64
           G GK  P+AIF++ FLGKA++ V IL   FP+LGL KE+C ETSW+E+ +IAAN F+ GE
Sbjct: 293 GGGKTNPLAIFFSLFLGKADELVTILNNPFPELGLSKEDCIETSWIEAALIAANGFQTGE 352

Query: 65  PVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYG 124
            +EALLNRSALIPPI S + K KSDYVKEPM + AIEG+WNR + SQ+I   +++F+PYG
Sbjct: 353 VLEALLNRSALIPPINSGRSKTKSDYVKEPMTRTAIEGMWNR-SKSQEIERASLVFIPYG 412

Query: 125 GRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPR 184
           G+MSEISESEIPF HRAGN Y+I Y+ GW++  +  E RHLNWIRE+YSYM PFVSKSPR
Sbjct: 413 GKMSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPR 472

Query: 185 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 244
           + YVN RDLDIGSN D YG   T++ +A  WGLKY+GNNF+RLV +KT+VDPY+FFRHEQ
Sbjct: 473 AVYVNCRDLDIGSN-DMYGR--TSYKRARVWGLKYFGNNFDRLVHVKTEVDPYDFFRHEQ 532

Query: 245 SIPT 248
           SIPT
Sbjct: 533 SIPT 532

BLAST of Chy12G210290 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 220.3 bits (560), Expect = 2.1e-57
Identity = 112/232 (48.28%), Postives = 150/232 (64.66%), Query Frame = 0

Query: 19  FLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGEPVEALLNRSALIPP 78
           FLG AN+ +++++  FPQLGL K++C ETSW++SV+  A  F    P EALL+  +L   
Sbjct: 319 FLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAG-FPSTAPSEALLDGKSLF-- 378

Query: 79  ITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEIPFS 138
                 K KSDYV+EP+P   +EG+W ++           ++ PYGG M++I E+E PF 
Sbjct: 379 --KNYFKAKSDYVEEPIPVEGLEGLWEKLLEED---SPLTIWNPYGGMMAKIPETETPFP 438

Query: 139 HRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSN 198
           HR+G L+KI +LT W+D     E++H+ W+RE+YSYM  +VSKSPRSAYVNYRDLD+G N
Sbjct: 439 HRSGTLFKIQWLTLWQDGKTS-EAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN 498

Query: 199 SDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPTAL 251
                        A  WG +Y+  NF RLV+IK K DP NFFRHEQSIPT L
Sbjct: 499 G--------KGSDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of Chy12G210290 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 220.3 bits (560), Expect = 2.1e-57
Identity = 111/236 (47.03%), Postives = 149/236 (63.14%), Query Frame = 0

Query: 15  FYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGEPVEALLNRSA 74
           +   FLG +N+ ++++++ FP+LGL K++C E SW++SV+  A  F +    EALL   +
Sbjct: 313 YQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAG-FPNSAAPEALLAGKS 372

Query: 75  LIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESE 134
           L         K KSD+VKEP+P   +EG+W R            ++ PYGG MS ISESE
Sbjct: 373 LF----KNHFKAKSDFVKEPIPVEGLEGLWERFLEED---SPLTIWNPYGGMMSRISESE 432

Query: 135 IPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLD 194
           IPF HR G L+KI +L+ W+D  +  E RH+ WIRE+YSYM  +VSK+PR AYVNYRDLD
Sbjct: 433 IPFPHRNGTLFKIQWLSTWQDGKVS-EERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLD 492

Query: 195 IGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPTAL 251
           +G+N             A  WG KYY  NF RLV+IK + DP NFFRHEQS+PT +
Sbjct: 493 LGTNE--------GETDAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of Chy12G210290 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 217.2 bits (552), Expect = 1.8e-56
Identity = 110/249 (44.18%), Postives = 158/249 (63.45%), Query Frame = 0

Query: 2   TSEGDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFK 61
           T E   K    A+F   ++G  N  + +++EKFP+LGL+KE C+E SW+ESV+  A DF 
Sbjct: 289 TLERSNKNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFA-DFP 348

Query: 62  DGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFV 121
            GE +  L NR       TS   K K D+V+EP+P+ AI+ IW R+   +   G  ++  
Sbjct: 349 KGESLGVLTNRER-----TSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLG-KIILT 408

Query: 122 PYGGRMSEISESEIPFSHRAGNLYKIAYLTGW---EDPSIDVESRHLNWIREIYSYMAPF 181
           P+GG+MSE++E E PF HR GNLY+I Y+  W   ED +     ++L W+  +Y +M P+
Sbjct: 409 PFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPY 468

Query: 182 VSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYN 241
           VSKSPR AYVN++D+D+G      G   T +++  SWG+KY+ NNF RLV++KT+VDP +
Sbjct: 469 VSKSPRGAYVNFKDMDLGM---YLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTD 527

Query: 242 FFRHEQSIP 248
           FF  EQSIP
Sbjct: 529 FFCDEQSIP 527

BLAST of Chy12G210290 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 215.7 bits (548), Expect = 5.1e-56
Identity = 113/243 (46.50%), Postives = 151/243 (62.14%), Query Frame = 0

Query: 6   DGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDGEP 65
           DG       +   FLG+    +K++++ FP+LGL +++C E SW+E+ +     F  G P
Sbjct: 307 DGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGG-FPTGSP 366

Query: 66  VEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVL-FVPYG 125
           +E LL    L  P+     K  SD+VKEP+P + ++GI+ R+      G    L + PYG
Sbjct: 367 IEILLQ---LKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIE----GNTTFLNWTPYG 426

Query: 126 GRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSPR 185
           G MS+I ES IPF HR G L+KI Y   W + +    SR +NWI+EIY+YMAP+VS +PR
Sbjct: 427 GMMSKIPESAIPFPHRNGTLFKILYYANWLE-NDKTSSRKINWIKEIYNYMAPYVSSNPR 486

Query: 186 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 245
            AYVNYRDLD G N +   N   N  +A  WG KY+  NF+RLV+IKTKVDP NFFRHEQ
Sbjct: 487 QAYVNYRDLDFGQNKN---NAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQ 537

Query: 246 SIP 248
           SIP
Sbjct: 547 SIP 537

BLAST of Chy12G210290 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 212.6 bits (540), Expect = 4.3e-55
Identity = 113/244 (46.31%), Postives = 156/244 (63.93%), Query Frame = 0

Query: 5   GDGKVKPVAIFYTS-FLGKANKAVKILKEKFPQLGLKKEECKETSWVESVVIAANDFKDG 64
           G G  K V   Y + FLG     + ++K+ FP+LGL  ++C E SW+ES+   +  F   
Sbjct: 302 GTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISG-FPTH 361

Query: 65  EPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPY 124
            P   LL   +  P ++    K KSD+VK P+P+  ++GI+ ++   +DI    +++ PY
Sbjct: 362 TPTNVLLQGKSPFPKVS---FKAKSDFVKTPIPESGLQGIFKKL-LKEDIP--LMIWNPY 421

Query: 125 GGRMSEISESEIPFSHRAGNLYKIAYLTGWEDPSIDVESRHLNWIREIYSYMAPFVSKSP 184
           GG M++I ES+IPF HR G L+K+ Y+T W D S    SRH+NWIR++YSYM P+VS +P
Sbjct: 422 GGMMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNP 481

Query: 185 RSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHE 244
           R AYVNYRDLD+G N+    ++ T   QA  WG  Y+ NNFNRL+ IK KVDP NFFRHE
Sbjct: 482 REAYVNYRDLDLGRNTK---DVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHE 534

Query: 245 QSIP 248
           QSIP
Sbjct: 542 QSIP 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93ZA32.9e-5648.28Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=... [more]
O647432.9e-5647.03Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV... [more]
Q9FI212.5e-5544.18Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=... [more]
Q9FKV07.2e-5546.50Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=... [more]
A6P6V93.6e-5445.97Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LYM23.7e-13996.11BBE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G540370 PE=4 SV... [more]
A0A6J1KJP12.3e-8865.57berberine bridge enzyme-like 27 OS=Cucurbita maxima OX=3661 GN=LOC111495223 PE=3... [more]
A0A6J1EMV22.3e-8865.98berberine bridge enzyme-like 27 OS=Cucurbita moschata OX=3662 GN=LOC111434009 PE... [more]
A0A0A0LY375.1e-8863.39BBE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G539350 PE=4 SV... [more]
A0A6J1DHZ21.2e-8463.97berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 P... [more]
Match NameE-valueIdentityDescription
KAE8653320.17.10e-17396.11hypothetical protein Csa_023331 [Cucumis sativus][more]
XP_004135739.11.58e-17296.11berberine bridge enzyme-like 28 [Cucumis sativus][more]
XP_023520064.12.03e-10665.16berberine bridge enzyme-like 27 [Cucurbita pepo subsp. pepo][more]
XP_022927075.13.83e-10665.98berberine bridge enzyme-like 27 [Cucurbita moschata][more]
XP_023000935.13.83e-10665.57berberine bridge enzyme-like 27 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G30760.12.1e-5748.28FAD-binding Berberine family protein [more]
AT2G34790.12.1e-5747.03FAD-binding Berberine family protein [more]
AT5G44440.11.8e-5644.18FAD-binding Berberine family protein [more]
AT5G44380.15.1e-5646.50FAD-binding Berberine family protein [more]
AT5G44400.14.3e-5546.31FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 186..247
e-value: 2.8E-22
score: 78.6
NoneNo IPR availableGENE3D3.40.462.20coord: 8..182
e-value: 5.8E-75
score: 255.0
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 12..248
NoneNo IPR availablePANTHERPTHR32448:SF113BERBERINE BRIDGE ENZYME-LIKE 18coord: 12..248
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 48..247
e-value: 5.8E-75
score: 255.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G210290.1Chy12G210290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding