Chy12G209210 (gene) Cucumber (hystrix) v1

BLAST of Chy12G209210 vs. ExPASy Swiss-Prot
Match: Q642R9 (Rab3 GTPase-activating protein catalytic subunit OS=Xenopus laevis OX=8355 GN=rab3gap1 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 5.8e-50
Identity = 230/902 (25.50%), Postives = 378/902 (41.91%), Query Frame = 0

Query: 18  DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGSTQLEISTNLYKVKSD-- 77
           + EV    DFT AS WERFIS++E V  +W   G  +   K   + E ++ +++ K +  
Sbjct: 8   ESEVFEITDFTTASEWERFISKVEEVLTEWKLIGETS--NKPPEKGEYTSGVWEEKGEEV 67

Query: 78  LKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLC---------------------FGVQ 137
           L  D +     +Y    +  K    +     + +C                     +G++
Sbjct: 68  LFADFRFSIRHHYLVQKSSEKEEKEDTGEDSIPVCMQDLLCTNNDFPPVAHCLVRWYGLR 127

Query: 138 EFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGT 197
           EF+VI+P +   VI +S + + LLS+V+IAL N     P FV VH   RK Y+G +  G 
Sbjct: 128 EFVVISPGANDAVISES-KCNLLLSSVSIALGNTGCQVPLFVQVHQKWRKLYVG-ECRGP 187

Query: 198 TFTRRFEADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLS 257
                FE   +  +VP +  HL GL ++F SK       +P     + FT       +L 
Sbjct: 188 GVRTDFEMVHL-RKVPNQYTHLSGLLDIFKSKIGCPLTALPPVNIAIRFT------YVLQ 247

Query: 258 DDDDI----RRADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASW-- 317
           D        +  D D     E   +   GK+ +          + EDP+    L  +W  
Sbjct: 248 DWQQYFWPQQPPDIDALIGGEVGGL-EFGKLPFG---------ACEDPISELHLATTWPC 307

Query: 318 -SEKMIESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDM 377
            +E +I  +   ++L+   P +A +W +  +   +     +G+   F S+ + L    + 
Sbjct: 308 LTEGIIVDNDVYSDLD---PLQAPQWSV-RVRKADNPQCMLGD---FVSEFFRLCRRKES 367

Query: 378 SFQAQFMEDFVSVENPGSD--NLKSSTVVPPPTVIDRV-LKELFHEGKKSHYFAKG---- 437
           + +      F      G+D     S    P P  I ++ +  + H  +K     +G    
Sbjct: 368 TDELLGKSAFEENGKEGADISQALSKLTEPAPVPIHKLSVTSMVHSARKKIRKHRGADES 427

Query: 438 --------------------------------GHRNSQA----------IKAAPVDSLFA 497
                                           G+ +SQ+          +K+AP +SL  
Sbjct: 428 PLNNDVLNAILFFLFPDTKSLDGSEAKPSTSTGNISSQSESEDYNLYSQLKSAPSNSLTY 487

Query: 498 Q--FCLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKL 557
           +   CL ++ F +  ++ +A LW EFV E+R+ WE    +P +  + S DL  CL++QKL
Sbjct: 488 KLALCLCMVNFYHGGVKGVAHLWQEFVLEMRYRWENNFLIPGL-ANGSPDLKCCLLHQKL 547

Query: 558 QMLAICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCD---- 617
           QML  C+ERK    E        +     +   +  V++  S + +P E    K +    
Sbjct: 548 QMLNCCLERKKARDE-------GKKGNPLYSSSESSVNKTASDLLSPVEADKSKYEVAKS 607

Query: 618 ----SHSSEDEFESKAN---LLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSY 677
               S S E+ FE  ++   L E   +S        +++P     G     GN+MLLNS 
Sbjct: 608 WDSWSDSEEEFFECHSDTEELKESGQESARKAKEETKENPSPKPEGRLHQSGNLMLLNSG 667

Query: 678 QIMHAPFTQDVPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPD 737
           + ++ P TQD   MT+D+ EE+ + +   G S +     A+++   L SDM +FKAANP 
Sbjct: 668 EPLYIPVTQDPAPMTDDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPG 727

Query: 738 GVFGDFIRWHSPGDWEEESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLW 797
               DF+RW+SP D+ EE    D            K N   +G LS RM    N+W + W
Sbjct: 728 CCLEDFVRWYSPRDYIEEEVMDD------------KGNKIFKGELSARMKIPNNMWVEAW 787

Query: 798 NDAPALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMV-CTSFKAAADTLSQTNYGG 824
             A  +P   Q+ L D  +E EK+LHYL   +P  L   ++ C    A      +     
Sbjct: 788 ETAKPIPARRQRRLFDDTKEAEKVLHYLAVQKPADLTRHLLPCVIHAALLKLKEEEAAED 847

BLAST of Chy12G209210 vs. ExPASy Swiss-Prot
Match: Q6NUV0 (Rab3 GTPase-activating protein catalytic subunit OS=Danio rerio OX=7955 GN=rab3gap1 PE=2 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 1.3e-49
Identity = 231/877 (26.34%), Postives = 371/877 (42.30%), Query Frame = 0

Query: 18  DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGP--NNLLKKG--------STQLEIS- 77
           + EV    DFT AS WERFIS +E V   W   G      L+KG         +  EIS 
Sbjct: 8   ESEVFEITDFTTASEWERFISRVEEVLNDWKLIGSRVGKPLEKGEYTSGTWEESSQEISF 67

Query: 78  -------TNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVV---DWELSLHELQLCFGVQEF 137
                  T+ Y  +   + D ++   E  +  +    +    D+    H L   FGV+EF
Sbjct: 68  ADFKFSITHHYLKQESAENDGRDELEEDAYPLAMQDLLCINNDFPPRAHCLVRWFGVREF 127

Query: 138 LVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTF 197
           +VI+P +    I+   + S LLS+V+IAL N     P FV +    RK + G +  G   
Sbjct: 128 VVISPGANCEAIISESKCSLLLSSVSIALANTGCQVPLFVQIQQKHRKMFSG-ECQGPGV 187

Query: 198 TRRFEADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDD 257
              FE   +  +VP +  HL GL ++F +K       +P     + FT  L      S  
Sbjct: 188 RSDFEMVHL-RRVPSQYNHLSGLLDIFKNKIGCPLTPLPPINISIRFTYVLQDWQQCSWP 247

Query: 258 DDIRRADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASW---SEKMI 317
              +  D D   + E   V   GK+ +          + EDP+    L A+W   +E ++
Sbjct: 248 Q--QPPDFDALLAGEVGGV-EFGKLPFG---------ACEDPISELHLAATWPSLTEGIV 307

Query: 318 ESSLEMAELEN-SSPH--------EAKKWILFPILS--------PNITDSTMGNRVGFS- 377
             +   ++L+   +PH        E  + +L   L+           T+  +G       
Sbjct: 308 VDNDVYSDLDPLQAPHWSVRVRTAENPQCLLGEFLTEFFKICCRKETTEEILGRSTAEEE 367

Query: 378 -----------SQL----------YLLIDALDMSFQAQFMEDFVSVENPGSDNLKSSTVV 437
                      S+L           L + ++  S + +        E+P ++++ +S ++
Sbjct: 368 GKENSDITQALSKLTEPSTAVPIHKLSVSSMVHSARKRIRRHRRVPESPLNNDVLNSILL 427

Query: 438 P--PPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQ--FCLHVLWFGNC 497
              P   +D+         ++S       +     +K+ P DSL  +   C+ ++ F + 
Sbjct: 428 YLFPDAALDKADVSEVRPPQQSPDRQSEDYHLYNQLKSCPSDSLTHRLALCICMVNFHHG 487

Query: 498 NIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLAICIERK--HQ 557
            +RA+A LW EFV E+R+ WE    L     S   DL  CL++QKLQML  CIERK    
Sbjct: 488 GVRAVAHLWQEFVLEMRYRWEN-NCLIYGLASGPPDLRCCLLHQKLQMLNCCIERKKARD 547

Query: 558 SIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSHSSEDEFESKANLLE 617
             ++     G++D      E  GP  E     +  G+++D   D   SEDEF    +  E
Sbjct: 548 DGKKSSSSDGARDRSRGAPEGAGP--EGAGPAEAAGKSWDSWSD---SEDEFFECVSDTE 607

Query: 618 DSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMTEDMHEERL 677
           +  + ++      +  P+    G     G   LLN+ + ++ P TQ+   MTED+ EE+ 
Sbjct: 608 EMKEDKEEAENRSRSKPE----GRLQPHGTHTLLNTQEPLYIPITQEPAPMTEDLLEEQS 667

Query: 678 QAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEEESGPKD 737
           + +   G S +     A+++   L SDM +FKAANP     DF+RW+SP D+ EE     
Sbjct: 668 EVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCTLLDFVRWYSPRDYVEEQ---- 727

Query: 738 EDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPNREGEK 797
                      +      RG LS RM   GN+W + W  A A P   QK L D  +E EK
Sbjct: 728 --------VTDADGRVEVRGELSARMKIPGNMWVEAWETARATPARRQKRLFDDTKEAEK 787

Query: 798 ILHYLETLRPHQLLEQMV-CTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASVLKFLQ 822
           +LHYL   +P +L   ++ C    A      + +   L  + + ++Q+ ++ + +L+   
Sbjct: 788 VLHYLALQKPSELTRHLLPCVLHAALLKIKEEESAEDLPSVRSSLQQISSSASKILRHPN 840

BLAST of Chy12G209210 vs. ExPASy Swiss-Prot
Match: Q80UJ7 (Rab3 GTPase-activating protein catalytic subunit OS=Mus musculus OX=10090 GN=Rab3gap1 PE=1 SV=4)

HSP 1 Score: 197.2 bits (500), Expect = 8.4e-49
Identity = 222/885 (25.08%), Postives = 357/885 (40.34%), Query Frame = 0

Query: 18  DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPN--NLLKKG----------STQLEI 77
           + EV    DFT AS WERFIS++E V   W   GP+    L+KG          S ++  
Sbjct: 8   ESEVFEITDFTTASEWERFISKVEEVLNDWKLIGPSLGKPLEKGIFTSGTWEERSDEISF 67

Query: 78  S------TNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVV---DWELSLHELQLCFGVQEF 137
           +      T+ Y V+     + K+  +E     S    +    D+    H L   +G++EF
Sbjct: 68  ADFRFSVTHHYLVQESPDKERKDEELEDAIPQSMQDLLCMNNDFPPRAHCLVRWYGLREF 127

Query: 138 LVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTF 197
           +VIAP + S  +L   + + LLS+++IAL N     P FV +H   R+ Y+G +  G   
Sbjct: 128 VVIAPAAHSDAVLSESKCNLLLSSISIALGNTGCQVPLFVQIHHKWRRMYMG-ECQGPGV 187

Query: 198 TRRFEADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDD 257
              FE   +  +VP +  HL GL ++F SK       +P     V   ++LT+  +L D 
Sbjct: 188 RTDFEMVHL-RKVPSQYTHLSGLLDIFKSKIGCPLTPLP----PVSIAIRLTY--VLQDW 247

Query: 258 DDI----RRADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKM 317
                  +  D D     E   +   GK+ +          + EDP+    L  +W    
Sbjct: 248 QQYFWPQQPPDIDALVGGEVGGL-EFGKLPFG---------ACEDPISELHLATTWPHLT 307

Query: 318 IESSLEMAELENSSPHEAKKWILFPILSPN--------------------ITDSTMGNR- 377
               ++     +  P +A  W +    + N                     TD  +G   
Sbjct: 308 EGIIVDNDVYSDLDPVQAPHWSVRVRKADNPQCLLGDFVTEFLKICRRKESTDEILGRST 367

Query: 378 --------VGFSSQLYLLIDALDMSFQAQFMEDFVSV------ENPGSDNLKSSTVVP-- 437
                      +  L  L +   +      + + V        ++ G ++  +S V+   
Sbjct: 368 FEEEGREVADITHALSKLTEPAPVPIHKLSVSNMVHTAKKKIRKHRGEESPLNSDVLNTI 427

Query: 438 -----PPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQ--FCLHVLWFG 497
                P  V ++ L               G +      K+AP DSL  +   CL ++ F 
Sbjct: 428 LLFLFPDAVSEKPLDGTTSIDNSIPAPEAGDYTLYNQFKSAPSDSLTYKLALCLCMINFY 487

Query: 498 NCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLAICIERKHQ 557
           +  ++ +A LW EFV E+R+ WE    +P +  S S DL  CL++QKLQML  CIERK  
Sbjct: 488 HGGLKGVAHLWQEFVLEMRFRWENNFLIPGL-ASGSPDLRCCLLHQKLQMLNCCIERKKA 547

Query: 558 SIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSHSSEDEFESKANLLE 617
             E  +  L S    S +  D G     + +        +      S +   +S+    E
Sbjct: 548 RDEGKKTSL-SDSTTSAYPGDAGKTGGQLGLDHLRDTEKEKGEVGKSWDSWSDSEEEFFE 607

Query: 618 DSTKSEDLISFTDQKSPDSMRRGSAGIV--------GNMMLLNSYQIMHAPFTQDVPLMT 677
             + +EDL     +       +  A +         G + LL++ + ++ P TQ+   MT
Sbjct: 608 CLSDTEDLKGNGQESGKKGGPKEMANLKPEGRLHQHGKLTLLHNGEPLYIPVTQEPAPMT 667

Query: 678 EDMHEERLQAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDW 737
           ED+ EE+ + +   G S +     A+++   L SDM +FKAANP     DF+RW+SP D+
Sbjct: 668 EDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCFLEDFVRWYSPRDY 727

Query: 738 EEESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLL 797
            EE                 K N   +G LS RM    N+W + W  A  +P   Q+ L 
Sbjct: 728 IEEE------------VTDEKGNVVLKGELSARMKIPSNMWVEAWETAKPVPARRQRRLF 787

Query: 798 DPNREGEKILHYLETLRPHQLLEQMV-CTSFKAAADTLSQTNYGGLKLMITKMEQLYTTM 822
           D  RE EK+LHYL   +P  L   ++ C    A      + +   +  +   ++Q+    
Sbjct: 788 DDTREAEKVLHYLAMQKPADLARHLLPCVIHAAVLKVKEEESLENIPSVKKIIKQIIAHS 847

BLAST of Chy12G209210 vs. ExPASy Swiss-Prot
Match: Q15042 (Rab3 GTPase-activating protein catalytic subunit OS=Homo sapiens OX=9606 GN=RAB3GAP1 PE=1 SV=3)

HSP 1 Score: 196.4 bits (498), Expect = 1.4e-48
Identity = 231/890 (25.96%), Postives = 368/890 (41.35%), Query Frame = 0

Query: 18  DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNL---LKKG----------STQLE 77
           + EV    DFT AS WERFIS++E V   W   G N+L   L+KG          S ++ 
Sbjct: 8   ESEVFEITDFTTASEWERFISKVEEVLNDWKLIG-NSLGKPLEKGIFTSGTWEEKSDEIS 67

Query: 78  IS------TNLYKVKSDLKCDNKNYSMEYYFGNSNH---GKVVDWELSLHELQLCFGVQE 137
            +      T+ Y V+     + K+  +E     S     G   D+    H L   +G++E
Sbjct: 68  FADFKFSVTHHYLVQESTDKEGKDELLEDVVPQSMQDLLGMNNDFPPRAHCLVRWYGLRE 127

Query: 138 FLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTT 197
           F+VIAP + S  +L   + + LLS+V+IAL N     P FV +H   R+ Y+G +  G  
Sbjct: 128 FVVIAPAAHSDAVLSESKCNLLLSSVSIALGNTGCQVPLFVQIHHKWRRMYVG-ECQGPG 187

Query: 198 FTRRFEADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSD 257
               FE   +  +VP +  HL GL ++F SK       +P     + FT       +L D
Sbjct: 188 VRTDFEMVHL-RKVPNQYTHLSGLLDIFKSKIGCPLTPLPPVSIAIRFT------YVLQD 247

Query: 258 DDDI----RRADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEK 317
                   +  D D     E   +   GK+ +          + EDP+    L  +W   
Sbjct: 248 WQQYFWPQQPPDIDALVGGEVGGL-EFGKLPFG---------ACEDPISELHLATTWPHL 307

Query: 318 MIESSLEMAELENSSPHEAKKWILFPILSPN--------------------ITDSTMGNR 377
                ++     +  P +A  W +    + N                     TD  +G R
Sbjct: 308 TEGIIVDNDVYSDLDPIQAPHWSVRVRKAENPQCLLGDFVTEFFKICRRKESTDEILG-R 367

Query: 378 VGFSSQ----------LYLLIDALDMSFQAQFMEDFV-----------SVENPGSDNLKS 437
             F  +          L  L +   +      + + V            VE    +N   
Sbjct: 368 SAFEEEGKETADITHALSKLTEPASVPIHKLSVSNMVHTAKKKIRKHRGVEESPLNNDVL 427

Query: 438 STVVP---PPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQ--FCLHVL 497
           +T++    P  V ++ L         +       +      K+AP DSL  +   CL ++
Sbjct: 428 NTILLFLFPDAVSEKPLDGTTSTDNNNPPSESEDYNLYNQFKSAPSDSLTYKLALCLCMI 487

Query: 498 WFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLAICIER 557
            F +  ++ +A LW EFV E+R+ WE    +P +  S   DL  CL++QKLQML  CIER
Sbjct: 488 NFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGL-ASGPPDLRCCLLHQKLQMLNCCIER 547

Query: 558 KHQSIEEFQDCLGSQDPESPHDEDDGPVSEDI---SIMQTP------GENFDGKCDSHSS 617
           K    E  +    + D  + +  D G   + +   ++ +T       G+++D   D  S 
Sbjct: 548 KKARDEGKK--TSASDVTNIYPGDAGKAGDQLVPDNLKETDKEKGEVGKSWDSWSD--SE 607

Query: 618 EDEFESKANLLEDSTKSEDLISFTDQKSPDSMR-RGSAGIVGNMMLLNSYQIMHAPFTQD 677
           E+ FE  ++  E     ++       K   ++R  G     G + LL++ + ++ P TQ+
Sbjct: 608 EEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEPLYIPVTQE 667

Query: 678 VPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPDGVFGDFIRWH 737
              MTED+ EE+ + +   G S +     A+++   L SDM +FKAANP     DF+RW+
Sbjct: 668 PAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCSLEDFVRWY 727

Query: 738 SPGDWEEESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSE 797
           SP D+ EE                 K N   +G LS RM    N+W + W  A  +P   
Sbjct: 728 SPRDYIEEE------------VIDEKGNVVLKGELSARMKIPSNMWVEAWETAKPIPARR 787

Query: 798 QKSLLDPNREGEKILHYLETLRPHQLLEQMV-CTSFKAAADTLSQTNYGGLKLMITKMEQ 822
           Q+ L D  RE EK+LHYL   +P  L   ++ C    A      + +   +  +   ++Q
Sbjct: 788 QRRLFDDTREAEKVLHYLAIQKPADLARHLLPCVIHAAVLKVKEEESLENISSVKKIIKQ 847

BLAST of Chy12G209210 vs. ExPASy Swiss-Prot
Match: Q9VQ26 (Rab3 GTPase-activating protein catalytic subunit OS=Drosophila melanogaster OX=7227 GN=CG31935 PE=1 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 9.0e-35
Identity = 212/862 (24.59%), Postives = 340/862 (39.44%), Query Frame = 0

Query: 14  EALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGSTQLEISTNLYKVK 73
           E +DD E  + ++F+  S WE F +++  + ++W     +      S ++  S N    +
Sbjct: 3   EEIDDNEF-YRENFSADSDWEVFNAQLGEILQKWDVSSDSETRNLKSEEI-FSCNWKVER 62

Query: 74  SDLKCDNKNYSMEYY-------------------------------FGNSNHGKVVDWEL 133
             L        +EY+                                GNS  G  +    
Sbjct: 63  EKLDMLRNGIEVEYHQAILEDEELVRAEAKEITCLQRTSCHHDLMSTGNS-FGPPIRSSQ 122

Query: 134 SLHELQLCFGVQEFLVIAPQSIS-GVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDP 193
            LH L   +G++ F+V+ P + +   +  + E +  LSAVA+      S  P FV ++DP
Sbjct: 123 ELHILARIYGLRRFIVLHPVNPTLNYMRSTSEFNFFLSAVAVVSAEVQSLVPIFVQIYDP 182

Query: 194 SRKAYIGIQNMGTTFTRRFEADRVGTQ-VPIKLMHLEGLYELFVSKFAYSSVDIPTNFFK 253
               Y G+  +       F    +G +  P +   L GL  LF  K       +PT++ +
Sbjct: 183 KWNYYTGVA-LAPALRTNFRL--IGLEKAPPECRFLMGLLTLFREK-------VPTSYTQ 242

Query: 254 VHFTMKLTFRILLSDDDDIRR---ADADMTESAEENAV-GTHGKVQWDDDCPWSEWYSSE 313
                  T   L  D   IR       D   S+E+  V G    ++    C     Y  E
Sbjct: 243 AAMISVCTTYAL--DTMRIRMPMYVPFDHGLSSEDIVVDGEVSHLEVQQFCALPHGYKPE 302

Query: 314 DPVKGFQLIASWSEKMIESSLEMAELENSSPHEAKKWILF------PILSPNITD----- 373
              +   L+ +W E     + +  +  +  P +A    ++        LS  + D     
Sbjct: 303 SRTE-IYLVYTWPELSEHVAFDSEQRSDFVPAKAPLGKIYLSVEASSYLSCCLRDYQSVA 362

Query: 374 -------STMG-NRVGFSSQLYLLIDALDMSFQAQFMEDFV-SVENPGSDNLKSSTVVP- 433
                  S +G N  G SS      + LD   + +  +    S E P    L      P 
Sbjct: 363 EVTRSLESFVGRNFSGTSSGAEAASNPLDRITEHKLTKRRERSFELPSQAGLTKRLPGPM 422

Query: 434 PPTVIDRVLKELF---HEGKKSHYFAKGGHRNS---QAIKAAPVDSLFAQF-CLHVLWFG 493
             + +  +L  LF   H       +AK    +      IK+A  DSL  +  CL      
Sbjct: 423 TESELSELLAYLFPDMHPEMALFPYAKKNFTDKFDPMRIKSAVPDSLVCRLSCLLATCHA 482

Query: 494 NC-NIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLAICIERKH 553
           +  ++  +A +W  F R++R  W+    +P +  +   D  +CL++QKLQML +C+ER+ 
Sbjct: 483 HLGSVEGMAQVWAAFTRQLRLLWDNSLMVPGIS-AGFPDTRTCLLHQKLQMLNVCVERRV 542

Query: 554 Q----SIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSHSSEDEFESK 613
           Q    S  + +  +G    E   DEDD             GE FD  CD  ++     +K
Sbjct: 543 QREANSKRKSEGMVGKASSEEEEDEDD-----------DEGEFFD--CDDLTAGAGSPTK 602

Query: 614 ANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMTEDM 673
           A L                K    +RR    +    +L    + ++ P TQ+    TED 
Sbjct: 603 AVL--------------SLKPEGRLRR----LNNERLLEEPDEYLYIPDTQEPVPKTEDQ 662

Query: 674 HEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEEESGP 733
            ++  + +   G     + Q+    L SDM AFKAANP G+  DFIRW+SP DWEE +  
Sbjct: 663 LQDDAEVMLKLGPGSGLTTQMMCTSLLSDMEAFKAANPRGIMEDFIRWYSPKDWEEVTDE 722

Query: 734 KDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPNREG 793
             +                 +  LS RM+  GN W+++W  A A+PVS QK L D   E 
Sbjct: 723 LGQ----------------VKHQLSIRMTTEGNTWQKVWEQAQAVPVSRQKRLFDDTNEA 782

Query: 794 EKILHYLETLRPHQLLEQMVCTSFKAA----ADTLSQTNYGGLKLMITKMEQLYTTMASV 802
            K+LHYLET + H++    V     +A    AD LS        L  +++E+L + +   
Sbjct: 783 LKVLHYLETRKMHEIYNLTVIPLLHSAILKLADILSNAELE--DLFSSQIEKLLSDLCR- 797

BLAST of Chy12G209210 vs. ExPASy TrEMBL
Match: A0A0A0LV89 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_1G533420 PE=3 SV=1)

HSP 1 Score: 1889.4 bits (4893), Expect = 0.0e+00
Identity = 936/953 (98.22%), Postives = 942/953 (98.85%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MASSTK ESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS
Sbjct: 1   MASSTKVESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNH KVVDWELSLHELQLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHDKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240
           EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKMIESSLEMA 300
           RADADMTESAEENA GTHGKVQWDDDCPWSEWYSSEDPVKGF+LIASWSEKMIESSLEMA
Sbjct: 241 RADADMTESAEENADGTHGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMIESSLEMA 300

Query: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360
           ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQ+YLLIDALDMSFQAQFMEDFVSV
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQMYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQF 420
           ENPGSDNLKSSTVVPPPTV+DRVLKELFHEGKK HYFAKG HRNSQAIKAAPVDSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVVDRVLKELFHEGKKFHYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSHSSEDEF 540
           ICIERKHQSIEEFQDCLGSQDPESP DEDDGPVSED S+MQTPGENFDGKCDSHSSEDEF
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGPVSEDFSLMQTPGENFDGKCDSHSSEDEF 540

Query: 541 ESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMT 600
           ESKANLLEDS+KSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMT
Sbjct: 541 ESKANLLEDSSKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMT 600

Query: 601 EDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEEE 660
           EDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF DFIRWHSPGDWEEE
Sbjct: 601 EDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEEE 660

Query: 661 SGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPN 720
           SGPKDEDLPD SATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPN
Sbjct: 661 SGPKDEDLPDSSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPN 720

Query: 721 REGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASVL 780
           REGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLM TKMEQLYTTMASVL
Sbjct: 721 REGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMKTKMEQLYTTMASVL 780

Query: 781 KFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDFY 840
           KFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDFY
Sbjct: 781 KFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDFY 840

Query: 841 LPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEPM 900
           LPRMGTGSSGSKF+TEFNKKQLVRSHERGVISSMF PPTASQSWRKVLSMGNLFNGHEPM
Sbjct: 841 LPRMGTGSSGSKFRTEFNKKQLVRSHERGVISSMFIPPTASQSWRKVLSMGNLFNGHEPM 900

Query: 901 LREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 954
           LREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD
Sbjct: 901 LREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 953

BLAST of Chy12G209210 vs. ExPASy TrEMBL
Match: A0A5A7UVM5 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00740 PE=3 SV=1)

HSP 1 Score: 1868.2 bits (4838), Expect = 0.0e+00
Identity = 927/954 (97.17%), Postives = 939/954 (98.43%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MASSTK ESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS
Sbjct: 1   MASSTKVESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNY+MEYYFGNSNHGKVVDWEL+LHELQLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYTMEYYFGNSNHGKVVDWELNLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240
           EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKMIESSLEMA 300
           RAD DM+ESA+ENAVGTHGKVQWDDDCPWSEWYSSEDPVKGF+LIASWSEKM+ESSLEMA
Sbjct: 241 RADTDMSESAQENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360
           ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFS+QLYLLIDALDMSFQAQFMEDFVSV
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSTQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQF 420
           ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKS YFAKG HRNSQAIKAAPVDSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSH-SSEDE 540
           ICIERKHQSIEEFQDCLGSQDPESP DEDDG VSED SIMQTPGENFDGKCDSH SSEDE
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGSVSEDFSIMQTPGENFDGKCDSHLSSEDE 540

Query: 541 FESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLM 600
            ESKANLL+DSTKSEDLI+F DQKSPDSMRRGSAGIVGNMMLLNS+QIMHAPFTQDVPLM
Sbjct: 541 LESKANLLKDSTKSEDLITFADQKSPDSMRRGSAGIVGNMMLLNSHQIMHAPFTQDVPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF DFIRWHSPGDWEE
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660

Query: 661 ESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           ESGPKDEDLPDGSATETSKNNW PRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 ESGPKDEDLPDGSATETSKNNWRPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASV 780
           NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLM TKMEQLY+TMASV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMKTKMEQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDF 840
           LKFLQGNRLSAESEV DDLRRLCVVFEHVEKLMALAASLHRK SQAPRLSEVIFKDYFDF
Sbjct: 781 LKFLQGNRLSAESEVFDDLRRLCVVFEHVEKLMALAASLHRKFSQAPRLSEVIFKDYFDF 840

Query: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900
           YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP
Sbjct: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 MLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 954
           MLREIVFSLRDRVNGNHYADSTPTA+EEEIDTHRMYINGTANDLRVALSVTSCD
Sbjct: 901 MLREIVFSLRDRVNGNHYADSTPTAREEEIDTHRMYINGTANDLRVALSVTSCD 954

BLAST of Chy12G209210 vs. ExPASy TrEMBL
Match: A0A1S3C6J8 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103497052 PE=3 SV=1)

HSP 1 Score: 1868.2 bits (4838), Expect = 0.0e+00
Identity = 927/954 (97.17%), Postives = 939/954 (98.43%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MASSTK ESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS
Sbjct: 1   MASSTKVESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNY+MEYYFGNSNHGKVVDWEL+LHELQLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYTMEYYFGNSNHGKVVDWELNLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240
           EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKMIESSLEMA 300
           RAD DM+ESA+ENAVGTHGKVQWDDDCPWSEWYSSEDPVKGF+LIASWSEKM+ESSLEMA
Sbjct: 241 RADTDMSESAQENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360
           ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFS+QLYLLIDALDMSFQAQFMEDFVSV
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSTQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQF 420
           ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKS YFAKG HRNSQAIKAAPVDSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSH-SSEDE 540
           ICIERKHQSIEEFQDCLGSQDPESP DEDDG VSED SIMQTPGENFDGKCDSH SSEDE
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGSVSEDFSIMQTPGENFDGKCDSHLSSEDE 540

Query: 541 FESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLM 600
            ESKANLL+DSTKSEDLI+F DQKSPDSMRRGSAGIVGNMMLLNS+QIMHAPFTQDVPLM
Sbjct: 541 LESKANLLKDSTKSEDLITFADQKSPDSMRRGSAGIVGNMMLLNSHQIMHAPFTQDVPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF DFIRWHSPGDWEE
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660

Query: 661 ESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           ESGPKDEDLPDGSATETSKNNW PRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 ESGPKDEDLPDGSATETSKNNWRPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASV 780
           NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLM TKMEQLY+TMASV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMKTKMEQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDF 840
           LKFLQGNRLSAESEV DDLRRLCVVFEHVEKLMALAASLHRK SQAPRLSEVIFKDYFDF
Sbjct: 781 LKFLQGNRLSAESEVFDDLRRLCVVFEHVEKLMALAASLHRKFSQAPRLSEVIFKDYFDF 840

Query: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900
           YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP
Sbjct: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 MLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 954
           MLREIVFSLRDRVNGNHYADSTPTA+EEEIDTHRMYINGTANDLRVALSVTSCD
Sbjct: 901 MLREIVFSLRDRVNGNHYADSTPTAREEEIDTHRMYINGTANDLRVALSVTSCD 954

BLAST of Chy12G209210 vs. ExPASy TrEMBL
Match: A0A6J1K9B0 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111492852 PE=3 SV=1)

HSP 1 Score: 1753.8 bits (4541), Expect = 0.0e+00
Identity = 869/954 (91.09%), Postives = 909/954 (95.28%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MASS+K ESNGEDEA++DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKK +
Sbjct: 1   MASSSKAESNGEDEAMEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120
           T LEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA
Sbjct: 61  TPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240
           EADR+GTQVPIKLMHLEGLYELFVSKFAYSS++I  N FKVHFTMKLTF+ILLSDDDDIR
Sbjct: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSMEISMNLFKVHFTMKLTFKILLSDDDDIR 240

Query: 241 RADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKMIESSLEMA 300
           R DA+MTES  E+AVGT GK QWDDDCPWSEWYSSEDPVKGF+LIASWSEKM+ESSLEMA
Sbjct: 241 RDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360
           ELENSSPHEAKKWILFPILSPNITD+TMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQF 420
           ENPGSDN KSST VPPPTVIDRVLKELFHEGKKS YF+KG HR SQAIKAA +DSLFAQF
Sbjct: 361 ENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFSKGEHRTSQAIKAAALDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLSSCLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSHSS-EDE 540
           ICIERKHQSIEEFQDCL SQDPESP  E+DGPVSED+S MQTPGENFDGKCDSHSS E E
Sbjct: 481 ICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDGKCDSHSSPEVE 540

Query: 541 FESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLM 600
           FESKANL ++ TKSED+ISFTDQKS DS RRGSAGIVG MMLLNSYQ MH+PFTQD PLM
Sbjct: 541 FESKANLFKERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSMHSPFTQDAPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF DFIRWHSP DW E
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPRDW-E 660

Query: 661 ESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           +S PKDE LP+GSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASV 780
           NREGEKILHYLETLRPHQLLEQMVCT+FKA ADTLSQT+YGGLKLM TKM+QLY+TMASV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTKMDQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKL QAPRLSE IFKDY++F
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLSEAIFKDYYEF 840

Query: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900
           YLPRMGTG S +KF TEFNKKQLVRSHERG ISS+FTPPTASQSWRKVLSMGNLFNGHEP
Sbjct: 841 YLPRMGTGMSSNKFDTEFNKKQLVRSHERGAISSLFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 MLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 954
           +LREI+FSLRDRV+GNHYADSTPTA+ E I+THRMYI+GT+NDLRVALSVTSCD
Sbjct: 901 ILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALSVTSCD 953

BLAST of Chy12G209210 vs. ExPASy TrEMBL
Match: A0A6J1GBV8 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111452560 PE=3 SV=1)

HSP 1 Score: 1752.6 bits (4538), Expect = 0.0e+00
Identity = 869/954 (91.09%), Postives = 907/954 (95.07%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MASS+K ESNG DEA++DEEVEHFDDFTIASTWERFISEIEAVCRQWM+DGPNNLLKK +
Sbjct: 1   MASSSKAESNGGDEAMEDEEVEHFDDFTIASTWERFISEIEAVCRQWMSDGPNNLLKKDA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120
           T LEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA
Sbjct: 61  TPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240
           EADR+GTQVPIKLMHLEGLYELFVSKFAYSS+DI  N FKVHFTMKLTF+ILLSDDDDIR
Sbjct: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSMDISMNLFKVHFTMKLTFKILLSDDDDIR 240

Query: 241 RADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKMIESSLEMA 300
           R DA+MTES  E+AVGT GK QWDDDCPWSEWYSSEDPVKGF+LIASWSEKM+ESSLEMA
Sbjct: 241 RDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360
           ELENSSPHEAKKWILFPILSPNITD+TMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQF 420
           ENPGSDN KSST VPPPTVIDRVLKELFHEGKKS YFAKG HR SQAIKAA +DSLFAQF
Sbjct: 361 ENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFAKGEHRTSQAIKAAALDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLSSCLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSH-SSEDE 540
           ICIERKHQSIEEFQDCL SQDPESP  E+DGPVSED+S MQTPGENFDGKCDSH S E E
Sbjct: 481 ICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDGKCDSHLSPEVE 540

Query: 541 FESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLM 600
           FESK NL E+ TKSED+ISFTDQKS DS RRGSAGIVG MMLLNSYQ MHAPFTQD PLM
Sbjct: 541 FESKGNLFEERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSMHAPFTQDAPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF DFIRWHSP DW E
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPRDW-E 660

Query: 661 ESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           +S PKDE LP+GSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASV 780
           NREGEKILHYLETLRPHQLLEQMVCT+FKA ADTLSQT+YGGLKLM TKM+QLY+TMASV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTKMDQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKL QAPRLSE IFKDY++F
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLSEAIFKDYYEF 840

Query: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900
           YLPRMGTG S +KF+TEFNKKQLVRSHERG ISS+FTPPTASQSWRKVLSMGNLFNGHEP
Sbjct: 841 YLPRMGTGMSSNKFETEFNKKQLVRSHERGAISSLFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 MLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 954
           +LREI+FSLRDRV+GNHYADSTPTA+ E I+THRMYI+GT+NDLRVALSVTSCD
Sbjct: 901 ILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALSVTSCD 953

BLAST of Chy12G209210 vs. NCBI nr
Match: XP_011658733.1 (rab3 GTPase-activating protein catalytic subunit isoform X1 [Cucumis sativus] >KGN65835.1 hypothetical protein Csa_023299 [Cucumis sativus])

HSP 1 Score: 1884 bits (4881), Expect = 0.0
Identity = 936/953 (98.22%), Postives = 942/953 (98.85%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MASSTK ESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS
Sbjct: 1   MASSTKVESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNH KVVDWELSLHELQLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHDKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240
           EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKMIESSLEMA 300
           RADADMTESAEENA GTHGKVQWDDDCPWSEWYSSEDPVKGF+LIASWSEKMIESSLEMA
Sbjct: 241 RADADMTESAEENADGTHGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMIESSLEMA 300

Query: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360
           ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQ+YLLIDALDMSFQAQFMEDFVSV
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQMYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQF 420
           ENPGSDNLKSSTVVPPPTV+DRVLKELFHEGKK HYFAKG HRNSQAIKAAPVDSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVVDRVLKELFHEGKKFHYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSHSSEDEF 540
           ICIERKHQSIEEFQDCLGSQDPESP DEDDGPVSED S+MQTPGENFDGKCDSHSSEDEF
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGPVSEDFSLMQTPGENFDGKCDSHSSEDEF 540

Query: 541 ESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMT 600
           ESKANLLEDS+KSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMT
Sbjct: 541 ESKANLLEDSSKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMT 600

Query: 601 EDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEEE 660
           EDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF DFIRWHSPGDWEEE
Sbjct: 601 EDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEEE 660

Query: 661 SGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPN 720
           SGPKDEDLPD SATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPN
Sbjct: 661 SGPKDEDLPDSSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPN 720

Query: 721 REGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASVL 780
           REGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLM TKMEQLYTTMASVL
Sbjct: 721 REGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMKTKMEQLYTTMASVL 780

Query: 781 KFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDFY 840
           KFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDFY
Sbjct: 781 KFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDFY 840

Query: 841 LPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEPM 900
           LPRMGTGSSGSKF+TEFNKKQLVRSHERGVISSMF PPTASQSWRKVLSMGNLFNGHEPM
Sbjct: 841 LPRMGTGSSGSKFRTEFNKKQLVRSHERGVISSMFIPPTASQSWRKVLSMGNLFNGHEPM 900

Query: 901 LREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 953
           LREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD
Sbjct: 901 LREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 953

BLAST of Chy12G209210 vs. NCBI nr
Match: XP_008457337.1 (PREDICTED: rab3 GTPase-activating protein catalytic subunit [Cucumis melo] >KAA0060023.1 rab3 GTPase-activating protein catalytic subunit [Cucumis melo var. makuwa] >TYJ97280.1 rab3 GTPase-activating protein catalytic subunit [Cucumis melo var. makuwa])

HSP 1 Score: 1863 bits (4826), Expect = 0.0
Identity = 927/954 (97.17%), Postives = 939/954 (98.43%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MASSTK ESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS
Sbjct: 1   MASSTKVESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNY+MEYYFGNSNHGKVVDWEL+LHELQLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYTMEYYFGNSNHGKVVDWELNLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240
           EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKMIESSLEMA 300
           RAD DM+ESA+ENAVGTHGKVQWDDDCPWSEWYSSEDPVKGF+LIASWSEKM+ESSLEMA
Sbjct: 241 RADTDMSESAQENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360
           ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFS+QLYLLIDALDMSFQAQFMEDFVSV
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSTQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQF 420
           ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKS YFAKG HRNSQAIKAAPVDSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSH-SSEDE 540
           ICIERKHQSIEEFQDCLGSQDPESP DEDDG VSED SIMQTPGENFDGKCDSH SSEDE
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGSVSEDFSIMQTPGENFDGKCDSHLSSEDE 540

Query: 541 FESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLM 600
            ESKANLL+DSTKSEDLI+F DQKSPDSMRRGSAGIVGNMMLLNS+QIMHAPFTQDVPLM
Sbjct: 541 LESKANLLKDSTKSEDLITFADQKSPDSMRRGSAGIVGNMMLLNSHQIMHAPFTQDVPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF DFIRWHSPGDWEE
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660

Query: 661 ESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           ESGPKDEDLPDGSATETSKNNW PRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 ESGPKDEDLPDGSATETSKNNWRPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASV 780
           NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLM TKMEQLY+TMASV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMKTKMEQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDF 840
           LKFLQGNRLSAESEV DDLRRLCVVFEHVEKLMALAASLHRK SQAPRLSEVIFKDYFDF
Sbjct: 781 LKFLQGNRLSAESEVFDDLRRLCVVFEHVEKLMALAASLHRKFSQAPRLSEVIFKDYFDF 840

Query: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900
           YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP
Sbjct: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 MLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 953
           MLREIVFSLRDRVNGNHYADSTPTA+EEEIDTHRMYINGTANDLRVALSVTSCD
Sbjct: 901 MLREIVFSLRDRVNGNHYADSTPTAREEEIDTHRMYINGTANDLRVALSVTSCD 954

BLAST of Chy12G209210 vs. NCBI nr
Match: XP_038894474.1 (rab3 GTPase-activating protein catalytic subunit isoform X3 [Benincasa hispida])

HSP 1 Score: 1833 bits (4747), Expect = 0.0
Identity = 908/954 (95.18%), Postives = 932/954 (97.69%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MASS K ESNGEDEAL+DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS
Sbjct: 1   MASSAKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHE+QLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHEIQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240
           EADRVGTQVPIKLMHLEGLYELFVSKFAYSS DIPTNFFKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSADIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKMIESSLEMA 300
           RADAD  ES +ENA+GTHGKVQWDDDCPW+EWYS EDPVKGF+LIASWSEKM+ESSLEMA
Sbjct: 241 RADADTIESVQENAIGTHGKVQWDDDCPWTEWYSLEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360
           ELENSSPHEAKKWILFPILSPNITD+T+GNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDNTIGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQF 420
           ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKS YFAKG HRNSQAIKAAPVDSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPR+P+SSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRIPVSSSIDLSTCLINQKLQMLA 480

Query: 481 ICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSH-SSEDE 540
           ICIERKHQSIEEFQDCLGSQDPESP DEDDGPVSED SIMQ PGENFDGK DSH SSEDE
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGPVSEDFSIMQMPGENFDGKSDSHLSSEDE 540

Query: 541 FESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLM 600
           FESK NLL+DSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQ MHAPFTQDVPLM
Sbjct: 541 FESKVNLLKDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQSMHAPFTQDVPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF DFIRWHSPGDWEE
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660

Query: 661 ESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           +SGP+DEDLP GSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 DSGPEDEDLPHGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASV 780
           NREGEKILHYLETLRPHQLLEQMVCT+F AAADTLSQT+YGGLKLM TKMEQLY TMASV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFNAAADTLSQTSYGGLKLMKTKMEQLYCTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASL+RKLSQAPRLSE+IFKDY+DF
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLYRKLSQAPRLSEMIFKDYYDF 840

Query: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900
           YLPRMGTGSSGSKF TEFNKKQLVR+HERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP
Sbjct: 841 YLPRMGTGSSGSKFGTEFNKKQLVRNHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 MLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 953
           +LREIVFSLRDRVNGNHYADSTPTA+EEEIDTHRMYINGT+NDLRVALSVTSCD
Sbjct: 901 ILREIVFSLRDRVNGNHYADSTPTAREEEIDTHRMYINGTSNDLRVALSVTSCD 954

BLAST of Chy12G209210 vs. NCBI nr
Match: XP_031745160.1 (rab3 GTPase-activating protein catalytic subunit isoform X2 [Cucumis sativus])

HSP 1 Score: 1822 bits (4719), Expect = 0.0
Identity = 903/919 (98.26%), Postives = 909/919 (98.91%), Query Frame = 0

Query: 35  RFISEIEAVCRQWMADGPNNLLKKGSTQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNH 94
           RFISEIEAVCRQWMADGPNNLLKKGSTQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNH
Sbjct: 11  RFISEIEAVCRQWMADGPNNLLKKGSTQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNH 70

Query: 95  GKVVDWELSLHELQLCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPA 154
            KVVDWELSLHELQLCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPA
Sbjct: 71  DKVVDWELSLHELQLCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPA 130

Query: 155 FVPVHDPSRKAYIGIQNMGTTFTRRFEADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDI 214
           FVPVHDPSRKAYIGIQNMGTTFTRRFEADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDI
Sbjct: 131 FVPVHDPSRKAYIGIQNMGTTFTRRFEADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDI 190

Query: 215 PTNFFKVHFTMKLTFRILLSDDDDIRRADADMTESAEENAVGTHGKVQWDDDCPWSEWYS 274
           PTNFFKVHFTMKLTFRILLSDDDDIRRADADMTESAEENA GTHGKVQWDDDCPWSEWYS
Sbjct: 191 PTNFFKVHFTMKLTFRILLSDDDDIRRADADMTESAEENADGTHGKVQWDDDCPWSEWYS 250

Query: 275 SEDPVKGFQLIASWSEKMIESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNRVGF 334
           SEDPVKGF+LIASWSEKMIESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNRVGF
Sbjct: 251 SEDPVKGFELIASWSEKMIESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNRVGF 310

Query: 335 SSQLYLLIDALDMSFQAQFMEDFVSVENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKS 394
           SSQ+YLLIDALDMSFQAQFMEDFVSVENPGSDNLKSSTVVPPPTV+DRVLKELFHEGKK 
Sbjct: 311 SSQMYLLIDALDMSFQAQFMEDFVSVENPGSDNLKSSTVVPPPTVVDRVLKELFHEGKKF 370

Query: 395 HYFAKGGHRNSQAIKAAPVDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQ 454
           HYFAKG HRNSQAIKAAPVDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQ
Sbjct: 371 HYFAKGEHRNSQAIKAAPVDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQ 430

Query: 455 PLPRMPISSSIDLSSCLINQKLQMLAICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVS 514
           PLPRMPISSSIDLSSCLINQKLQMLAICIERKHQSIEEFQDCLGSQDPESP DEDDGPVS
Sbjct: 431 PLPRMPISSSIDLSSCLINQKLQMLAICIERKHQSIEEFQDCLGSQDPESPRDEDDGPVS 490

Query: 515 EDISIMQTPGENFDGKCDSHSSEDEFESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAG 574
           ED S+MQTPGENFDGKCDSHSSEDEFESKANLLEDS+KSEDLISFTDQKSPDSMRRGSAG
Sbjct: 491 EDFSLMQTPGENFDGKCDSHSSEDEFESKANLLEDSSKSEDLISFTDQKSPDSMRRGSAG 550

Query: 575 IVGNMMLLNSYQIMHAPFTQDVPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMS 634
           IVGNMMLLNSYQIMHAPFTQDVPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMS
Sbjct: 551 IVGNMMLLNSYQIMHAPFTQDVPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMS 610

Query: 635 AFKAANPDGVFGDFIRWHSPGDWEEESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEH 694
           AFKAANPDGVF DFIRWHSPGDWEEESGPKDEDLPD SATETSKNNWPPRGHLSKRMSEH
Sbjct: 611 AFKAANPDGVFEDFIRWHSPGDWEEESGPKDEDLPDSSATETSKNNWPPRGHLSKRMSEH 670

Query: 695 GNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTSFKAAADTL 754
           GNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTSFKAAADTL
Sbjct: 671 GNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTSFKAAADTL 730

Query: 755 SQTNYGGLKLMITKMEQLYTTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMAL 814
           SQTNYGGLKLM TKMEQLYTTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMAL
Sbjct: 731 SQTNYGGLKLMKTKMEQLYTTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMAL 790

Query: 815 AASLHRKLSQAPRLSEVIFKDYFDFYLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSM 874
           AASLHRKLSQAPRLSEVIFKDYFDFYLPRMGTGSSGSKF+TEFNKKQLVRSHERGVISSM
Sbjct: 791 AASLHRKLSQAPRLSEVIFKDYFDFYLPRMGTGSSGSKFRTEFNKKQLVRSHERGVISSM 850

Query: 875 FTPPTASQSWRKVLSMGNLFNGHEPMLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRM 934
           F PPTASQSWRKVLSMGNLFNGHEPMLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRM
Sbjct: 851 FIPPTASQSWRKVLSMGNLFNGHEPMLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRM 910

Query: 935 YINGTANDLRVALSVTSCD 953
           YINGTANDLRVALSVTSCD
Sbjct: 911 YINGTANDLRVALSVTSCD 929

BLAST of Chy12G209210 vs. NCBI nr
Match: XP_022998101.1 (rab3 GTPase-activating protein catalytic subunit isoform X1 [Cucurbita maxima])

HSP 1 Score: 1749 bits (4530), Expect = 0.0
Identity = 869/954 (91.09%), Postives = 909/954 (95.28%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MASS+K ESNGEDEA++DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKK +
Sbjct: 1   MASSSKAESNGEDEAMEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120
           T LEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA
Sbjct: 61  TPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240
           EADR+GTQVPIKLMHLEGLYELFVSKFAYSS++I  N FKVHFTMKLTF+ILLSDDDDIR
Sbjct: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSMEISMNLFKVHFTMKLTFKILLSDDDDIR 240

Query: 241 RADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKMIESSLEMA 300
           R DA+MTES  E+AVGT GK QWDDDCPWSEWYSSEDPVKGF+LIASWSEKM+ESSLEMA
Sbjct: 241 RDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360
           ELENSSPHEAKKWILFPILSPNITD+TMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAPVDSLFAQF 420
           ENPGSDN KSST VPPPTVIDRVLKELFHEGKKS YF+KG HR SQAIKAA +DSLFAQF
Sbjct: 361 ENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFSKGEHRTSQAIKAAALDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLSSCLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSHSS-EDE 540
           ICIERKHQSIEEFQDCL SQDPESP  E+DGPVSED+S MQTPGENFDGKCDSHSS E E
Sbjct: 481 ICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDGKCDSHSSPEVE 540

Query: 541 FESKANLLEDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLM 600
           FESKANL ++ TKSED+ISFTDQKS DS RRGSAGIVG MMLLNSYQ MH+PFTQD PLM
Sbjct: 541 FESKANLFKERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSMHSPFTQDAPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF DFIRWHSP DWE+
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPRDWED 660

Query: 661 ESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
            S PKDE LP+GSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 -SDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASV 780
           NREGEKILHYLETLRPHQLLEQMVCT+FKA ADTLSQT+YGGLKLM TKM+QLY+TMASV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTKMDQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKL QAPRLSE IFKDY++F
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLSEAIFKDYYEF 840

Query: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900
           YLPRMGTG S +KF TEFNKKQLVRSHERG ISS+FTPPTASQSWRKVLSMGNLFNGHEP
Sbjct: 841 YLPRMGTGMSSNKFDTEFNKKQLVRSHERGAISSLFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 MLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 953
           +LREI+FSLRDRV+GNHYADSTPTA+ E I+THRMYI+GT+NDLRVALSVTSCD
Sbjct: 901 ILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALSVTSCD 953

BLAST of Chy12G209210 vs. TAIR 10
Match: AT5G58510.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G55060.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 596/978 (60.94%), Postives = 739/978 (75.56%), Query Frame = 0

Query: 1   MASSTKFESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60
           MAS +K     ED+   +EEV+HFDDFT+AS+WERFIS+IEA CRQW+ADGP NL++KG+
Sbjct: 1   MASVSKSNHLEEDDDA-EEEVQHFDDFTLASSWERFISDIEATCRQWLADGPKNLVEKGA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGK----VVDWELSLHELQLCFGVQEF 120
             +E S NL+ VK +LK   K+Y ME+YF   N+G     + +W  + H+LQLCFGV++F
Sbjct: 61  VAVEDSKNLFTVKHELKNVAKSYCMEFYFQIDNNGSQQAGIGNWNSNSHDLQLCFGVKDF 120

Query: 121 LVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTF 180
           L+IAPQS SGV+LD+PE+SKLLSAVAIAL+NC S WPAFVPVHDPSRKAYIGIQNMGT F
Sbjct: 121 LLIAPQSASGVLLDTPESSKLLSAVAIALSNCGSLWPAFVPVHDPSRKAYIGIQNMGTVF 180

Query: 181 TRRFEADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDD 240
           TRRFEADRVG+QVP+KLMHLEGLYELFVSKF YS VD   + F+VHF M+LT++    D+
Sbjct: 181 TRRFEADRVGSQVPVKLMHLEGLYELFVSKFVYSGVDFSMHTFRVHFMMRLTYQTFPYDE 240

Query: 241 D----DIRRADADMTESAEENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFQLIASWSEKM 300
           +    D      D +++AE     +  KV WDDDCPWSEWYS+EDP++GF+L+ +W+++ 
Sbjct: 241 EDEEIDTDEVMGDKSDTAEHYGSESRNKVLWDDDCPWSEWYSAEDPLRGFELVVTWADRT 300

Query: 301 IESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQLYLLIDALDMSFQAQ 360
           +ES+LEMAELEN+SPH+A+KWIL PILSP + D + G R+ F+SQL  L++ALD SF AQ
Sbjct: 301 VESTLEMAELENASPHDAEKWILHPILSPYLGDPSHGKRIDFASQLLCLVEALDSSFSAQ 360

Query: 361 FMEDFVSVENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSHYFAKGGHRNSQAIKAAP 420
           FMEDFVSVENP S+NLK+S V+PPP+V+DRV+K+LF EG K   F KG HR S+A+KAAP
Sbjct: 361 FMEDFVSVENPSSENLKTSVVIPPPSVLDRVIKDLFREGSKLPDFTKGEHRLSRALKAAP 420

Query: 421 VDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLI 480
           ++SLF QFCLH LWFGNCNIRAIA LW+EFVREVRWCWEE QPLP+MPI  SIDLSSCLI
Sbjct: 421 LESLFTQFCLHSLWFGNCNIRAIAFLWIEFVREVRWCWEETQPLPKMPIDGSIDLSSCLI 480

Query: 481 NQKLQMLAICIERKHQSIEEFQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCD 540
           NQKL +LAICIE+K +  EEF DC+GS D            S D S+        D K  
Sbjct: 481 NQKLHLLAICIEKKREMNEEFLDCIGSDD------------SSDASVSMEEHHKVD-KRR 540

Query: 541 SHSSEDEFESK--ANLLEDSTK------SEDLISFTDQKSPDSMRRGSAGIVGNMMLLNS 600
           + SSE+E   K  +++ ED++K        +  +  +Q   D++RRGSAG VG MMLL S
Sbjct: 541 NTSSEEELRRKRDSSIAEDTSKQLRFERKTERTNSVNQSPTDAIRRGSAGPVGTMMLLKS 600

Query: 601 YQIMHAPFTQDVPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGV 660
            Q +HAPFTQD PLMTEDMHEERLQAVEAFGDS +   QLEKDIL SDMSAFKAANPD V
Sbjct: 601 RQQLHAPFTQDPPLMTEDMHEERLQAVEAFGDSLNVPGQLEKDILLSDMSAFKAANPDAV 660

Query: 661 FGDFIRWHSPGDWEEESGPKDEDLPDGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWND 720
           F DFIRWHSPGDWE    PK  +   G +TE SK+ WPPRG LS+RMS+ GNLWR+ WND
Sbjct: 661 FEDFIRWHSPGDWESFE-PKTTEPSAGPSTEGSKDEWPPRGRLSQRMSDQGNLWRKSWND 720

Query: 721 APALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKL 780
           APALP  +QK LLDPNREGEKI+HYLET+RPHQLLEQMVCT+F+ +ADTL+QTN G ++ 
Sbjct: 721 APALPADDQKPLLDPNREGEKIVHYLETVRPHQLLEQMVCTAFRGSADTLNQTNVGNMRQ 780

Query: 781 MITKMEQLYTTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQ 840
           M +K+EQLY  M   L  LQ N L  +++ + DL+RLC+VFE+VEKL+A+AAS+HRK   
Sbjct: 781 MTSKLEQLYLIMKFTLGALQRNNLPDKAKTVKDLKRLCMVFENVEKLVAVAASIHRKFLD 840

Query: 841 APRLSEVIFKDYFDFYLPRMGTGSSG--SKFKTEFN-KKQLVRSHERGVISSMFTPPTAS 900
           A RL++VIF D++  Y P MG  ++   +K +TE    +Q V   ER V+S++F+PP+A+
Sbjct: 841 ASRLAQVIFSDFYGVYAPTMGMSANDEENKSRTEMEVSRQEVSLRERQVVSNLFSPPSAN 900

Query: 901 QSWRKVLSMGNLFNGHEPMLREIVFSLRDRV-NGNHYADSTPTA-----QEEEIDTHRMY 954
           QSWRKVLSMGNL NGHEP+LREI+FS  D V NG HYA +   A     + EEI+THRMY
Sbjct: 901 QSWRKVLSMGNLLNGHEPILREIIFSTGDDVNNGIHYAAAADVAATSDRKGEEIETHRMY 960

BLAST of Chy12G209210 vs. TAIR 10
Match: AT5G55060.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1); Has 223 Blast hits to 218 proteins in 80 species: Archae - 0; Bacteria - 2; Metazoa - 117; Fungi - 6; Plants - 65; Viruses - 0; Other Eukaryotes - 33 (source: NCBI BLink). )

HSP 1 Score: 147.1 bits (370), Expect = 7.1e-35
Identity = 120/396 (30.30%), Postives = 180/396 (45.45%), Query Frame = 0

Query: 433 RAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLAICIERKHQSIEE 492
           R +A  W   V E+R  W E + +P +P+ ++ DL SCL++Q LQ++  C+ RK +++  
Sbjct: 261 RRMALFWCRVVEELRRFWNEEKHIPWIPLDNNPDLKSCLLHQWLQVINCCLARKARNV-- 320

Query: 493 FQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSHSSEDEFESKANLLEDSTK 552
                                SE +  +     +      ++   D  ES  +LL   + 
Sbjct: 321 -------------------AASEALDAVMRQASS------ANEKSDVSESMGSLLYAKSN 380

Query: 553 SEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMTEDMHEERLQAVE 612
           S +LI          +R G    V N+ +L + + +++P TQ+ PL+TED+  E  + V 
Sbjct: 381 SGELI----------LRLGVYNQVENLTMLETGEPVYSPITQEGPLLTEDLIRETEELVL 440

Query: 613 AFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEEESGPKDEDLPDGS 672
             G      +Q     L SDM AFKAANP  +  DF+RWHSP DW E      +D     
Sbjct: 441 RTGSMGAGCSQ-----LLSDMQAFKAANPGCILEDFVRWHSPPDWTENGNMSGDD----- 500

Query: 673 ATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLET 732
                  + P RG LS RM + GNLWR+LW  A  LP  +Q  L D +   E IL+YLE 
Sbjct: 501 -------SSPVRGQLSTRMQKEGNLWRELWETAKPLPAVKQAPLFDEDLAVEGILNYLED 560

Query: 733 LRPHQLLEQ----MVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASVLKFLQGNRL 792
           +   +L EQ    +V   F      L+ TN    KL        +     V+   QG   
Sbjct: 561 IPAAELFEQLFTSLVSLGFVMVEPVLA-TNDDLSKL-------FFECKDYVVAICQG--- 588

Query: 793 SAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQ 825
            A ++ +DD   LC V+E VE ++     + R + Q
Sbjct: 621 GAWTDKLDD---LCQVYETVETMLLRPEEVLRSMKQ 588

BLAST of Chy12G209210 vs. TAIR 10
Match: AT5G55060.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1). )

HSP 1 Score: 147.1 bits (370), Expect = 7.1e-35
Identity = 120/396 (30.30%), Postives = 180/396 (45.45%), Query Frame = 0

Query: 433 RAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLAICIERKHQSIEE 492
           R +A  W   V E+R  W E + +P +P+ ++ DL SCL++Q LQ++  C+ RK +++  
Sbjct: 168 RRMALFWCRVVEELRRFWNEEKHIPWIPLDNNPDLKSCLLHQWLQVINCCLARKARNV-- 227

Query: 493 FQDCLGSQDPESPHDEDDGPVSEDISIMQTPGENFDGKCDSHSSEDEFESKANLLEDSTK 552
                                SE +  +     +      ++   D  ES  +LL   + 
Sbjct: 228 -------------------AASEALDAVMRQASS------ANEKSDVSESMGSLLYAKSN 287

Query: 553 SEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLMTEDMHEERLQAVE 612
           S +LI          +R G    V N+ +L + + +++P TQ+ PL+TED+  E  + V 
Sbjct: 288 SGELI----------LRLGVYNQVENLTMLETGEPVYSPITQEGPLLTEDLIRETEELVL 347

Query: 613 AFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFGDFIRWHSPGDWEEESGPKDEDLPDGS 672
             G      +Q     L SDM AFKAANP  +  DF+RWHSP DW E      +D     
Sbjct: 348 RTGSMGAGCSQ-----LLSDMQAFKAANPGCILEDFVRWHSPPDWTENGNMSGDD----- 407

Query: 673 ATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLET 732
                  + P RG LS RM + GNLWR+LW  A  LP  +Q  L D +   E IL+YLE 
Sbjct: 408 -------SSPVRGQLSTRMQKEGNLWRELWETAKPLPAVKQAPLFDEDLAVEGILNYLED 467

Query: 733 LRPHQLLEQ----MVCTSFKAAADTLSQTNYGGLKLMITKMEQLYTTMASVLKFLQGNRL 792
           +   +L EQ    +V   F      L+ TN    KL        +     V+   QG   
Sbjct: 468 IPAAELFEQLFTSLVSLGFVMVEPVLA-TNDDLSKL-------FFECKDYVVAICQG--- 495

Query: 793 SAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQ 825
            A ++ +DD   LC V+E VE ++     + R + Q
Sbjct: 528 GAWTDKLDD---LCQVYETVETMLLRPEEVLRSMKQ 495