Chy12G208650 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G208650
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	ervatamin-B-like
Location	chrH12: 2975660 .. 2977073 (+)
RNA-Seq Expression	Chy12G208650
Synteny	Chy12G208650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCATGATGAAATTTCTTATTGTTTCCCTTGTTTTGATTGCTTTCACCTCTCACCTATGTAAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGACGGACAAACGTTTCAAGGTTTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAATTTGCTGATATGTCTGATGATGAGTTTAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGCTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGAAAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCAGATGTGGTAATTAATTTAATTTAATTTATAATTCTTTCTAAACTCTAATGCCTTCTAACTAATAATGGTAATTAATTTAATTTAATTTATAATTCTTTCTAAACTCTAATGCCTTCTAACTAATAATTATGTGTTATGTGTTCCGCAGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAATATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGGAAAATGGTGGAGTCACAATTGAGGATAACTATCCGTATTATGCCGGAGATGGATATTGTCGTAGACCAGGAGTGAGTTTAAATCAATATAATTCTAATTAAACTATCTAAATTTATTATATAAGTGAGTTATTGATCATAATATAATTATGTGTATTGTGTTTGAATTATACTTCACTAATAGCCTAATAATGAGAGAGTGACAATTGATGGATACGAGAACGTACCTCGAAACAATGAGTATGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAGTGGAAGTGACTTTAGATTTTATGGAGAGGCAAGTGTAGTTAATTTTGTTGTTATAATTTAATTATAATTTAATTATAATTTGGTGCAACATAATGAAAAGAAAAAATTGATAATTATTTGCAGGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTACGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGGAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCACAAAGCCCACAAGGTGTATGTGGAATGGCAATGCAACCTGCCTTTCCCGTCAAGTATTGATTTCCTATCTCTTTTTTATTA

mRNA sequence

ATGACCATGATGAAATTTCTTATTGTTTCCCTTGTTTTGATTGCTTTCACCTCTCACCTATGTAAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGACGGACAAACGTTTCAAGGTTTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAATTTGCTGATATGTCTGATGATGAGTTTAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGCTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGAAAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCAGATGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAATATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGGAAAATGGTGGAGTCACAATTGAGGATAACTATCCGTATTATGCCGGAGATGGATATTGTCGTAGACCAGGACCTAATAATGAGAGAGTGACAATTGATGGATACGAGAACGTACCTCGAAACAATGAGTATGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAGTGGAAGTGACTTTAGATTTTATGGAGAGGCAAGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTACGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGGAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCACAAAGCCCACAAGGTGTATGTGGAATGGCAATGCAACCTGCCTTTCCCGTCAAGTATTGATTTCCTATCTCTTTTTTATTA

Coding sequence (CDS)

ATGACCATGATGAAATTTCTTATTGTTTCCCTTGTTTTGATTGCTTTCACCTCTCACCTATGTAAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGACGGACAAACGTTTCAAGGTTTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAATTTGCTGATATGTCTGATGATGAGTTTAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGCTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGAAAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCAGATGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAATATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGGAAAATGGTGGAGTCACAATTGAGGATAACTATCCGTATTATGCCGGAGATGGATATTGTCGTAGACCAGGACCTAATAATGAGAGAGTGACAATTGATGGATACGAGAACGTACCTCGAAACAATGAGTATGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAGTGGAAGTGACTTTAGATTTTATGGAGAGGCAAGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTACGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGGAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCACAAAGCCCACAAGGTGTATGTGGAATGGCAATGCAACCTGCCTTTCCCGTCAAGTATTGATTTCCTATCTCTTTTTTATTA

Protein sequence

MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGEARNVYGRELLRN*N*PHGSGSWVRK**RGRLLDNKEPIWNSMGNEWLYEDATRSTKPTRCMWNGNATCLSRQVLISYLFFIX

Homology

[Close]

BLAST of Chy12G208650 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 9.4e-78
Identity = 149/275 (54.18%), Postives = 194/275 (70.55%), Query Frame = 0


Query: 3   MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 62
           M KF++++L L A    + +SF+   K+ ESE+SL  LY+RW SHH +SR+ HE  KRF 
Sbjct: 1   MQKFILLALSL-ALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 63  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGF 122
           VFK NA HV   N M K  KLKLN+FADM++ EF  TY GS + +++       G  G F
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGN-GTF 120

Query: 123 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 182
           MYE+   +P+S+DWRKKGAV ++K+QG+CGSCWAF+ + AVE I+QIKTN+L+SLSEQE+
Sbjct: 121 MYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQEL 180

Query: 183 VDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 242
           VDCD     GC GG  + AFEFI + GG+T E NYPY A DG C     N   V+IDG+E
Sbjct: 181 VDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHE 240

Query: 243 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           NVP N+E AL+KAVA+QPV+V+I + GSDF+FY E
Sbjct: 241 NVPENDENALLKAVANQPVSVAIDAGGSDFQFYSE 273

[Close]

BLAST of Chy12G208650 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.0e-76
Identity = 150/277 (54.15%), Postives = 191/277 (68.95%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           M M K L V L L +    +  SF+   KD ESE+SL  LY+RW SHH +SR+  E  KR
Sbjct: 1   MAMKKLLWVVLSL-SLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVG 120
           F VFK N  HV   N M K  KLKLN+FADM++ EF  TY GS + ++K       G  G
Sbjct: 61  FNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGS-G 120

Query: 121 GFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQ 180
            FMYE+  ++P+S+DWRKKGAV  +K+QG+CGSCWAF+ + AVE I+QIKTN+L+SLSEQ
Sbjct: 121 TFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQ 180

Query: 181 EVVDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDG 240
           E+VDCD +   GC GG   SAFEFI + GG+T E NYPY A +G C     N+  V+IDG
Sbjct: 181 ELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDG 240

Query: 241 YENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           +ENVP N+E AL+KAVA+QPV+V+I + GSDF+FY E
Sbjct: 241 HENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSE 275

[Close]

BLAST of Chy12G208650 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 280.0 bits (715), Expect = 3.7e-74
Identity = 138/257 (53.70%), Postives = 177/257 (68.87%), Query Frame = 0


Query: 20  LCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNAKHVFKVNHMGK 79
           +  SF+   KD  SE+SL  LY+RW SHH +SR+  E  KRF VFK N  HV   N M K
Sbjct: 19  VANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVFKANLMHVHNTNKMDK 78

Query: 80  SLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYERATNIPSSIDWRKKG 139
             KLKLN+FADM++ EF  TY  +   +  +        G FMYE+  ++P S+DWRKKG
Sbjct: 79  PYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGAFMYEKVVSVPPSVDWRKKG 138

Query: 140 AVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYKVG-GCRGGDYNS 199
           AV  +K+QG+CGSCWAF+ V AVE I+QIKTN+L++LSEQE+VDCD +   GC GG   S
Sbjct: 139 AVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDCDKEENQGCNGGLMES 198

Query: 200 AFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNNEYALMKAVAHQP 259
           AFEFI + GG+T E NYPY A +G C     N+  V+IDG+ENVP N+E AL+KAVA+QP
Sbjct: 199 AFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVPANDEDALLKAVANQP 258

Query: 260 VAVSIASSGSDFRFYGE 276
           V+V+I + GSDF+FY E
Sbjct: 259 VSVAIDAGGSDFQFYSE 275

[Close]

BLAST of Chy12G208650 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 5.3e-73
Identity = 143/276 (51.81%), Postives = 188/276 (68.12%), Query Frame = 0


Query: 3   MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 62
           M K L++ L  +      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E +KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHA-KAGGRVGG 122
           VF+ N  HV   N   +S KLKLN+FAD++ +EF   Y GSNI +++ L   K G +   
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 182
           + +E  + +PSS+DWRKKGAV  IKNQG+CGSCWAF+ VAAVE I++IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGY 242
           +VDCD K   GC GG    AFEFI +NGG+T ED+YPY   DG C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           E+VP N+E AL+KAVA+QPV+V+I +  SDF+FY E
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE 275

[Close]

BLAST of Chy12G208650 vs. ExPASy Swiss-Prot
Match: Q9STL5 (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.5e-72
Identity = 139/270 (51.48%), Postives = 187/270 (69.26%), Query Frame = 0


Query: 11  LVLIAFTSHL--CKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNA 70
           +VLI+F S L   K F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF VF+ N 
Sbjct: 6   IVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRHNV 65

Query: 71  KHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGFMYERAT 130
            HV + N   K  KLK+N+FAD++  EF  +Y GSN+ +++ L     G  GGFMYE  T
Sbjct: 66  LHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYENVT 125

Query: 131 NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYK 190
            +PSS+DWR+KGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+L+SLSEQE+VDCD +
Sbjct: 126 RVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCDTE 185

Query: 191 VG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGD-GYCRRPGPNNERVTIDGYENVPRN 250
              GC GG    AFEFI  NGG+  E+ YPY + D  +CR      E VTIDG+E+VP N
Sbjct: 186 ENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVPEN 245

Query: 251 NEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           +E  L+KAVAHQPV+V+I +  SDF+ Y E
Sbjct: 246 DEEELLKAVAHQPVSVAIDAGSSDFQLYSE 274

[Close]

BLAST of Chy12G208650 vs. ExPASy TrEMBL
Match: A0A1S3BYQ3 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 7.3e-134
Identity = 239/274 (87.23%), Postives = 253/274 (92.34%), Query Frame = 0


Query: 2   TMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRF 61
           T+MKFLIV LVLIA TSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGF 121
           KVFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  G VGGF
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT-GHVGGF 122

Query: 122 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 181
           MYE A  IPSSIDWRKKGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQEV
Sbjct: 123 MYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 182

Query: 182 VDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYEN 241
           VDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERV IDGYEN
Sbjct: 183 VDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYEN 242

Query: 242 VPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           VPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 243 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 275

[Close]

BLAST of Chy12G208650 vs. ExPASy TrEMBL
Match: A0A1S3BYU0 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.6e-133
Identity = 239/275 (86.91%), Postives = 255/275 (92.73%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           M +MKFLIV LVLIAFT HLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FKVFKDNAK+VFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  GRVGG
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK-NGRVGG 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYE A +IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDY+  GC GG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERVTIDGYE
Sbjct: 181 VVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           NVPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 274

[Close]

BLAST of Chy12G208650 vs. ExPASy TrEMBL
Match: A0A5A7TM64 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003430 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 2.1e-133
Identity = 238/274 (86.86%), Postives = 252/274 (91.97%), Query Frame = 0


Query: 2   TMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRF 61
           T+MKFLIV  VLIA TSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KRF
Sbjct: 3   TVMKFLIVPFVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGF 121
           KVFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  G VGGF
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT-GHVGGF 122

Query: 122 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 181
           MYE A  IPSSIDWRKKGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQEV
Sbjct: 123 MYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 182

Query: 182 VDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYEN 241
           VDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERV IDGYEN
Sbjct: 183 VDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYEN 242

Query: 242 VPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           VPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 243 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 275

[Close]

BLAST of Chy12G208650 vs. ExPASy TrEMBL
Match: A0A5A7U0D8 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00020 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 4.4e-131
Identity = 237/284 (83.45%), Postives = 256/284 (90.14%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           MT+MKF IV+LVLIAF SHLC+SF+LERKDF SEKSLMQLYKRWSSHHRISRNA+E  KR
Sbjct: 1   MTVMKFFIVALVLIAFMSHLCESFKLERKDFASEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FKVFKDNAKHVFKVN MG+SLKL+LNQFADMSDDEFS  + SNITYYK+LHAK  G  G 
Sbjct: 61  FKVFKDNAKHVFKVNQMGRSLKLRLNQFADMSDDEFSSIHSSNITYYKHLHAKTVG--GS 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYE A  IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYEHAKEIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERVTIDGYE
Sbjct: 181 VVDCDYRDGGCRGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGEARNV-YGRE 284
           NVPRNNE+ALMKAVAHQPVAV+IAS GSDF+FYG+A  V YG +
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASGGSDFKFYGQAMVVGYGTD 282

[Close]

BLAST of Chy12G208650 vs. ExPASy TrEMBL
Match: A0A0A0KGB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 8.4e-130
Identity = 232/278 (83.45%), Postives = 252/278 (90.65%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           MT+MKFLIV LVL+AF+ ++C+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E   R
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAK----AGG 120
           FKVFK+NAKHVFKVN MGKSLKLKLNQFADMSDDEF   Y SNITYYK+LHAK     GG
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGG 120

Query: 121 RVGGFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISL 180
           R+GGFMYE A NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SL
Sbjct: 121 RIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 180

Query: 181 SEQEVVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTI 240
           SE+EV+DCDY+ GGCRGG YNSAFEF+M+N GVTIEDNYPYY G+GYCRR G  N+RV I
Sbjct: 181 SEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRI 240

Query: 241 DGYENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYG 275
           DGYENVPRNNEYALMKAVAHQPVAV+IAS GSDF+FYG
Sbjct: 241 DGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYG 278

[Close]

BLAST of Chy12G208650 vs. NCBI nr
Match: XP_031739597.1 (ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529 [Cucumis sativus])

HSP 1 Score: 507 bits (1306), Expect = 6.45e-178
Identity = 247/277 (89.17%), Postives = 264/277 (95.31%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           MTMMKFLIV +VLIAFTSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNAHE  KR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGG--RV 120
           FK+F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y NLHAKAGG  RV
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120

Query: 121 GGFMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSE 180
           GGFMYERA  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSE
Sbjct: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDG 240
           QEVVDCDYKVGGCRGGDYNSAFEFIM+NGG+TIE+NYPY+AG+GYCRR GPNNERVTIDG
Sbjct: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240

Query: 241 YENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 275
           YE VPRNNEYALMKAVAHQPVAV++ASSGSDFRFYGE
Sbjct: 241 YERVPRNNEYALMKAVAHQPVAVAVASSGSDFRFYGE 277

[Close]

BLAST of Chy12G208650 vs. NCBI nr
Match: XP_031740503.1 (ervatamin-B [Cucumis sativus])

HSP 1 Score: 504 bits (1299), Expect = 6.97e-177
Identity = 243/275 (88.36%), Postives = 261/275 (94.91%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           MTMMKFLIV +VLIAF SHLC+ F+LERKDFESEKSLMQLYKRWSSHHRISRNAHE  KR
Sbjct: 1   MTMMKFLIVFVVLIAFASHLCEGFDLERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FK+F+DNAK VFKVNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y NLHAKAGGRVGG
Sbjct: 61  FKIFQDNAKRVFKVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNNLHAKAGGRVGG 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYERA NIP SIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYERAMNIPFSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDYKVGGCRGGDYNSAFEFIM+NGG+TIE+NYPY+AG+GYCRR GPN+ERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 275
            VP+NNEYALMKAVAHQPVAVS+ASSGSDFRFYGE
Sbjct: 241 RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGE 275

[Close]

BLAST of Chy12G208650 vs. NCBI nr
Match: XP_031740474.1 (ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cucumis sativus])

HSP 1 Score: 489 bits (1260), Expect = 5.37e-171
Identity = 237/275 (86.18%), Postives = 261/275 (94.91%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           MTMMKFLIV +VLIAFTSHLC+SFELE KDFESE+SLMQLYKRWSSHHRISRNAHE  KR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FK+F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y +LHA    RVGG
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN---RVGG 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYERA NIPSSIDWR++GAVNAIKNQGRCGSCWAFAAVAAVESIHQI+TNEL+SLSEQE
Sbjct: 121 FMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDYKVGGCRGG+Y+SAFEFIM+NGG+TIE+NYPY+AG+GYCRR GPN+ERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 275
            VP+NNEYALMKAVAHQPVAVS+ASSGSDFRFYGE
Sbjct: 241 CVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGE 272

[Close]

BLAST of Chy12G208650 vs. NCBI nr
Match: XP_008454483.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 486 bits (1250), Expect = 2.06e-169
Identity = 239/274 (87.23%), Postives = 253/274 (92.34%), Query Frame = 0


Query: 2   TMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRF 61
           T+MKFLIV LVLIA TSHLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGF 121
           KVFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK G  VGGF
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKTG-HVGGF 122

Query: 122 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 181
           MYE A  IPSSIDWRKKGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNEL+SLSEQEV
Sbjct: 123 MYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 182

Query: 182 VDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYEN 241
           VDCDY+ GGCRGG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERV IDGYEN
Sbjct: 183 VDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYEN 242

Query: 242 VPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 275
           VPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 243 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 275

[Close]

BLAST of Chy12G208650 vs. NCBI nr
Match: XP_008454482.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 485 bits (1248), Expect = 4.00e-169
Identity = 239/275 (86.91%), Postives = 255/275 (92.73%), Query Frame = 0


Query: 1   MTMMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKR 60
           M +MKFLIV LVLIAFT HLC+SFELERKDFESEKSLMQLYKRWSSHHRISRNA+E  KR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGG 120
           FKVFKDNAK+VFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  GRVGG
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK-NGRVGG 120

Query: 121 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 180
           FMYE A +IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNEL+SLSEQE
Sbjct: 121 FMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 240
           VVDCDY+  GC GG YNSAFEF+MENGG+T+EDNYPYY GDGYCRR G  NERVTIDGYE
Sbjct: 181 VVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYE 240

Query: 241 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 275
           NVPRNNE+ALMKAVAHQPVAV+IASSGSDFRFYG+
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ 274

[Close]

BLAST of Chy12G208650 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.8e-74
Identity = 143/276 (51.81%), Postives = 188/276 (68.12%), Query Frame = 0


Query: 3   MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 62
           M K L++ L  +      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E +KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHA-KAGGRVGG 122
           VF+ N  HV   N   +S KLKLN+FAD++ +EF   Y GSNI +++ L   K G +   
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 FMYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQE 182
           + +E  + +PSS+DWRKKGAV  IKNQG+CGSCWAF+ VAAVE I++IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGY 242
           +VDCD K   GC GG    AFEFI +NGG+T ED+YPY   DG C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ENVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           E+VP N+E AL+KAVA+QPV+V+I +  SDF+FY E
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE 275

[Close]

BLAST of Chy12G208650 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 274.6 bits (701), Expect = 1.1e-73
Identity = 139/270 (51.48%), Postives = 187/270 (69.26%), Query Frame = 0


Query: 11  LVLIAFTSHL--CKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFKVFKDNA 70
           +VLI+F S L   K F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF VF+ N 
Sbjct: 6   IVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRHNV 65

Query: 71  KHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGFMYERAT 130
            HV + N   K  KLK+N+FAD++  EF  +Y GSN+ +++ L     G  GGFMYE  T
Sbjct: 66  LHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYENVT 125

Query: 131 NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYK 190
            +PSS+DWR+KGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+L+SLSEQE+VDCD +
Sbjct: 126 RVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCDTE 185

Query: 191 VG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGD-GYCRRPGPNNERVTIDGYENVPRN 250
              GC GG    AFEFI  NGG+  E+ YPY + D  +CR      E VTIDG+E+VP N
Sbjct: 186 ENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVPEN 245

Query: 251 NEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           +E  L+KAVAHQPV+V+I +  SDF+ Y E
Sbjct: 246 DEEELLKAVAHQPVSVAIDAGSSDFQLYSE 274

[Close]

BLAST of Chy12G208650 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 266.2 bits (679), Expect = 3.9e-71
Identity = 138/275 (50.18%), Postives = 183/275 (66.55%), Query Frame = 0


Query: 3   MMKFLIVSLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRWSSHHRISRNAHETDKRFK 62
           M +F++++L ++       K  +   KD ESE SL +LY+RW SHH ++R+  E  KRF 
Sbjct: 1   MKRFIVLALCML-MVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFN 60

Query: 63  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGF 122
           VFK N KH+ + N   KS KLKLN+F DM+ +EF +TY GSNI +++    +       F
Sbjct: 61  VFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKA-TKSF 120

Query: 123 MYERATNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEV 182
           MY     +P+S+DWRK GAV  +KNQG+CGSCWAF+ V AVE I+QI+T +L SLSEQE+
Sbjct: 121 MYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQEL 180

Query: 183 VDCDYKVG-GCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYE 242
           VDCD     GC GG  + AFEFI E GG+T E  YPY A D  C     N   V+IDG+E
Sbjct: 181 VDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHE 240

Query: 243 NVPRNNEYALMKAVAHQPVAVSIASSGSDFRFYGE 276
           +VP+N+E  LMKAVA+QPV+V+I + GSDF+FY E
Sbjct: 241 DVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSE 273

[Close]

BLAST of Chy12G208650 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 219.9 bits (559), Expect = 3.2e-57
Identity = 118/267 (44.19%), Postives = 167/267 (62.55%), Query Frame = 0


Query: 10  SLVLIAFTSHLCKSFELERKDFESEKSLMQLYKRW-SSHHRISRNAHETDKRFKVFKDNA 69
           SL L   +SH         +D ES   L++L++ W S+  +      E   RF+VFKDN 
Sbjct: 20  SLSLSFASSHDYSIVGYSPEDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNL 79

Query: 70  KHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKAGGRVGGFMYERAT 129
           KH+ + N  GKS  L LN+FAD+S +EF K Y G      +    ++      F Y    
Sbjct: 80  KHIDETNKKGKSYWLGLNEFADLSHEEFKKMYLGLKTDIVRRDEERS---YAEFAYRDVE 139

Query: 130 NIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDYK 189
            +P S+DWRKKGAV  +KNQG CGSCWAF+ VAAVE I++I T  L +LSEQE++DCD  
Sbjct: 140 AVPKSVDWRKKGAVAEVKNQGSCGSCWAFSTVAAVEGINKIVTGNLTTLSEQELIDCDTT 199

Query: 190 V-GGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNN 249
              GC GG  + AFE+I++NGG+  E++YPY   +G C      +E VTI+G+++VP N+
Sbjct: 200 YNNGCNGGLMDYAFEYIVKNGGLRKEEDYPYSMEEGTCEMQKDESETVTINGHQDVPTND 259

Query: 250 EYALMKAVAHQPVAVSIASSGSDFRFY 274
           E +L+KA+AHQP++V+I +SG +F+FY
Sbjct: 260 EKSLLKALAHQPLSVAIDASGREFQFY 283

[Close]

BLAST of Chy12G208650 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 216.9 bits (551), Expect = 2.7e-56
Identity = 117/267 (43.82%), Postives = 165/267 (61.80%), Query Frame = 0


Query: 12  VLIAFTSHLCKSFELERKDFESEKSLMQLYKRW-SSHHRISRNAHETDKRFKVFKDNAKH 71
           + +A  S  C S  L R   ++E  + + +  W + H R+  +  E + R+ VFK+N + 
Sbjct: 10  LFVAIFSSFCFSITLSR-PLDNELIMQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVER 69

Query: 72  VFKVNHM--GKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKAGGRVGGFMYERATN 131
           +  +N +  G++ KL +NQFAD+++DEF   Y +       L +++  ++  F Y+  ++
Sbjct: 70  IEHLNSIPAGRTFKLAVNQFADLTNDEFRSMY-TGFKGVSALSSQSQTKMSPFRYQNVSS 129

Query: 132 --IPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELISLSEQEVVDCDY 191
             +P S+DWRKKGAV  IKNQG CG CWAF+AVAA+E   QIK  +LISLSEQ++VDCD 
Sbjct: 130 GALPVSVDWRKKGAVTPIKNQGSCGCCWAFSAVAAIEGATQIKKGKLISLSEQQLVDCDT 189

Query: 192 KVGGCRGGDYNSAFEFIMENGGVTIEDNYPYYAGDGYCRRPGPNNERVTIDGYENVPRNN 251
              GC GG  ++AFE I   GG+T E NYPY   D  C     N +  +I GYE+VP N+
Sbjct: 190 NDFGCEGGLMDTAFEHIKATGGLTTESNYPYKGEDATCNSKKTNPKATSITGYEDVPVND 249

Query: 252 EYALMKAVAHQPVAVSIASSGSDFRFY 274
           E ALMKAVAHQPV+V I   G DF+FY
Sbjct: 250 EQALMKAVAHQPVSVGIEGGGFDFQFY 274

The following BLAST results are available for this feature:

BLAST of Chy12G208650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65039	9.4e-78	54.18	Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1	[more]
P12412	3.0e-76	54.15	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
P25803	3.7e-74	53.70	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
Q9STL4	5.3e-73	51.81	KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ...	[more]
Q9STL5	1.5e-72	51.48	KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ...	[more]

BLAST of Chy12G208650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BYQ3	7.3e-134	87.23	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1	[more]
A0A1S3BYU0	1.6e-133	86.91	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1	[more]
A0A5A7TM64	2.1e-133	86.86	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0034...	[more]
A0A5A7U0D8	4.4e-131	83.45	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00...	[more]
A0A0A0KGB1	8.4e-130	83.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1	[more]

BLAST of Chy12G208650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739597.1	6.45e-178	89.17	ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529...	[more]
XP_031740503.1	6.97e-177	88.36	ervatamin-B [Cucumis sativus]	[more]
XP_031740474.1	5.37e-171	86.18	ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cuc...	[more]
XP_008454483.1	2.06e-169	87.23	PREDICTED: ervatamin-B-like [Cucumis melo]	[more]
XP_008454482.1	4.00e-169	86.91	PREDICTED: ervatamin-B-like [Cucumis melo]	[more]

BLAST of Chy12G208650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48340.1	3.8e-74	51.81	Cysteine proteinases superfamily protein	[more]
AT3G48350.1	1.1e-73	51.48	Cysteine proteinases superfamily protein	[more]
AT5G50260.1	3.9e-71	50.18	Cysteine proteinases superfamily protein	[more]
AT1G20850.1	3.2e-57	44.19	xylem cysteine peptidase 2	[more]
AT5G45890.1	2.7e-56	43.82	senescence-associated gene 12	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 129..329 e-value: 4.3E-48 score: 175.8
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 129..276 e-value: 1.2E-46 score: 159.5
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 41..96 e-value: 1.0E-8 score: 45.0
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 41..96 e-value: 1.3E-9 score: 38.3
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 14..317 e-value: 9.6E-78 score: 264.1
None	No IPR available	PANTHER	PTHR12411:SF710	THIOL PROTEASE SEN102-LIKE	coord: 22..292
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 22..292
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 147..158
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 130..273 e-value: 6.99979E-64 score: 200.158
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 34..275

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G208650.1	Chy12G208650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0004713	protein tyrosine kinase activity
molecular_function	GO:0008234	cysteine-type peptidase activity