Chy12G206540 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G206540
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	chrH12: 487736 .. 489798 (-)
RNA-Seq Expression	Chy12G206540
Synteny	Chy12G206540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAAATTGGGAACCAGTGAAGGCTTTAGGCCAAGGTTCATGTGCCAGTGTGTTCTTAGCACGATCAACAATGGATCCTCTCGATTATTTTGCTGTTAAAGTAGCTCGCCTTCGAAACAAATCTTCATTGTCGTGGGAAAAGAATATTTTGAAACATTTTATTGGATGCGCAGAAATTGTTCAATGTTTAGGGAGTGAGGTGACTTTAAATGGAGATCACCTTCTTTACAATCTCAAGTTGGAATACGCTGCTGGAGGAACTTTAGCTGATTTGATTCAACAACGAAAAAAGTTATCAGAGGATGAAGTGAAGGAGTATCTCAAGATGATCTTAAAAGGGGTCTCTTGTATTCATCGCAAAGGGTTTGTGCATGCCGATTTGAAGCCAGCTAACATTCTTGCATTCCCTCAAAATAATGGGAAGATGGAGTTGAAGATTGCTGACTTTGGGATTTCGAGGAGATGTGGTGAGAAGCATGAAGTGGCCAAGGATGCTAGTCGTTGTTCAAAGTTATTTAAAGGAACACCATATTATATGTCACCTGAATCTATTACTTCTAAGGTGTTCAACAGTGCTCTTGATATTTGGTCATTGGGTTGCATTTTAATTCAAATGGTTTCTGAAGAACCAGTGTGGGAGGATTTCTCAAGTATCACATATCTGGTGACAAAACTTGTATCTGACATGGAAACACCGAAAATACCTGAAGAATTATCTGAGAAAGGAAAGGATTTTATTAAAAGATGCTTTGATCGAGGCCCTAAACAACGATGGACAGCTGATATGTTACTTGAACATCCCTATCTTAGAAAAGATCAAAAGGAAACCAGAACTAGAAGTATTCTAAAAAAAAATGGTTATATTTTGTTAATAGTTTGTAATATTCTTAGTTAGACCATTCATGTTAATGCAACTATAATCAAAATTATTGTTATTAAATGTTAAACTTTCTTATTTTTACATTATTTTCATGAAAATCAATTAGTCAATAAATTGAATTTAAGTATAAAATATTTTTAAAAAGTCAATTAAACATTTTATATATGATAAATTTGGGATATACAATTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGATTAGTTAGGTCTAGAAATTCAAAATCAACACTCCAAGCACAAACATTAGAAATCCAGATTACTAATTCATGTACCACAAACATAGGCATATGTAATTTTTAAGCATCAAAGTTTCATCAAATAAGTCTACAATCATTTAATTTTTAGACATTTGATTTCGAACGTGCAGGTTGATGAAAAGAGTAACTAGGGAGAGGTTATGAGAAAGGTGAAAATGATTAAAGGGTCAGCTCCAATTATTCTAACGTTTGACTTGAAAAGTAAGAAAGTGGAAACAAACAAACCCAATCCATCAAAACGACACCGCTTCTATCTCTTCAATTTTAATTTTTAATTCAACCTCTTCCGTGGGCCCCACTTCCCCAAATTTAAATTTAAATTCCAACCTGGAAAATTATAAAATTATTAAAGAAAAAGAAAATGAAAAAGTGCATTCAATTAATAATAATAGATGATGTCTTCTCGGACTCCATTTCTCTTTTTGATTTATCATAATCTAATGTTTATGTCCACCTCCCCGGCGCACGTGTGAAGACTTCAACACAACCATCCCAATAAAATAATCTAAATTAAAACACTAATTAGTTCATTAAAATTTTTAAGGTAGAAAGATTAGACCTATAAACAATCATCTATCTCATCTCAATCTCTTACCTTTCGTGCTAAAACTTTAAATTAGATGTGAACTAAATATAGGATTAGATTTATAGATTTGTGATATAGCTCGTTAGAAAAGTGTTTTTTTAACCGTCAGTGAACCTAAATATTGGAAAGCTCATCATTTTGAAGAAACTTACTTATTTTTGTTTGTTGTGTCAAGAAAAGAAAAACAAATTTGTAGTCAGTGTCCAAACTAGAGAAGAATTTGTGAATCACATCAAAACGTTTATGGAGCTCAATTACCATAGCATTTCTCTCAAAGCAGGTGTATTGTACAACTCACATAACTAG

mRNA sequence

ATGGTAAATTGGGAACCAGTGAAGGCTTTAGGCCAAGGTTCATGTGCCAGTGTGTTCTTAGCACGATCAACAATGGATCCTCTCGATTATTTTGCTGTTAAAGTAGCTCGCCTTCGAAACAAATCTTCATTGTCGTGGGAAAAGAATATTTTGAAACATTTTATTGGATGCGCAGAAATTGTTCAATGTTTAGGGAGTGAGGTGACTTTAAATGGAGATCACCTTCTTTACAATCTCAAGTTGGAATACGCTGCTGGAGGAACTTTAGCTGATTTGATTCAACAACGAAAAAAGTTATCAGAGGATGAAGTGAAGGAGTATCTCAAGATGATCTTAAAAGGGGTCTCTTGTATTCATCGCAAAGGGTTTGTGCATGCCGATTTGAAGCCAGCTAACATTCTTGCATTCCCTCAAAATAATGGGAAGATGGAGTTGAAGATTGCTGACTTTGGGATTTCGAGGAGATGTGGTGAGAAGCATGAAGTGGCCAAGGATGCTAGTCGTTGTTCAAAGTTATTTAAAGGAACACCATATTATATGTCACCTGAATCTATTACTTCTAAGGTGTTCAACAGTGCTCTTGATATTTGGTCATTGGGTTGCATTTTAATTCAAATGGTTTCTGAAGAACCAGTGTGGGAGGATTTCTCAAGTATCACATATCTGGTGACAAAACTTGTATCTGACATGGAAACACCGAAAATACCTGAAGAATTATCTGAGAAAGGAAAGGATTTTATTAAAAGATGCTTTGATCGAGGCCCTAAACAACGATGGACAGCTGATATGTTACTTGAACATCCCTATCTTAGAAAAGATCAAAAGGAAACCAGAACTAGAAAAAAGAAAAACAAATTTGTAGTCAGTGTCCAAACTAGAGAAGAATTTGTGAATCACATCAAAACGTTTATGGAGCTCAATTACCATAGCATTTCTCTCAAAGCAGGTGTATTGTACAACTCACATAACTAG

Coding sequence (CDS)

ATGGTAAATTGGGAACCAGTGAAGGCTTTAGGCCAAGGTTCATGTGCCAGTGTGTTCTTAGCACGATCAACAATGGATCCTCTCGATTATTTTGCTGTTAAAGTAGCTCGCCTTCGAAACAAATCTTCATTGTCGTGGGAAAAGAATATTTTGAAACATTTTATTGGATGCGCAGAAATTGTTCAATGTTTAGGGAGTGAGGTGACTTTAAATGGAGATCACCTTCTTTACAATCTCAAGTTGGAATACGCTGCTGGAGGAACTTTAGCTGATTTGATTCAACAACGAAAAAAGTTATCAGAGGATGAAGTGAAGGAGTATCTCAAGATGATCTTAAAAGGGGTCTCTTGTATTCATCGCAAAGGGTTTGTGCATGCCGATTTGAAGCCAGCTAACATTCTTGCATTCCCTCAAAATAATGGGAAGATGGAGTTGAAGATTGCTGACTTTGGGATTTCGAGGAGATGTGGTGAGAAGCATGAAGTGGCCAAGGATGCTAGTCGTTGTTCAAAGTTATTTAAAGGAACACCATATTATATGTCACCTGAATCTATTACTTCTAAGGTGTTCAACAGTGCTCTTGATATTTGGTCATTGGGTTGCATTTTAATTCAAATGGTTTCTGAAGAACCAGTGTGGGAGGATTTCTCAAGTATCACATATCTGGTGACAAAACTTGTATCTGACATGGAAACACCGAAAATACCTGAAGAATTATCTGAGAAAGGAAAGGATTTTATTAAAAGATGCTTTGATCGAGGCCCTAAACAACGATGGACAGCTGATATGTTACTTGAACATCCCTATCTTAGAAAAGATCAAAAGGAAACCAGAACTAGAAAAAAGAAAAACAAATTTGTAGTCAGTGTCCAAACTAGAGAAGAATTTGTGAATCACATCAAAACGTTTATGGAGCTCAATTACCATAGCATTTCTCTCAAAGCAGGTGTATTGTACAACTCACATAACTAG

Protein sequence

MVNWEPVKALGQGSCASVFLARSTMDPLDYFAVKVARLRNKSSLSWEKNILKHFIGCAEIVQCLGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILKGVSCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFKGTPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETPKIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKETRTRKKKNKFVVSVQTREEFVNHIKTFMELNYHSISLKAGVLYNSHN*

Homology

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BLAST of Chy12G206540 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-45
Identity = 113/328 (34.45%), Postives = 184/328 (56.10%), Query Frame = 0


Query: 2   VNWEPVKALGQGSCASVFLARSTMDPLDY---FAVKVARLRNKSSLSWEKNILKHFIGCA 61
           + W   + +G G+ ++V  A  + +  D+    AVK       +SLS EK++L     C 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 62  EIVQCLGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQ--RKKLSEDEVKEYLKMILKGVS 121
           EI++C G + T+     ++NL LEYA+ G+LA  +++   + L E  V+ +   +L+G+ 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 122 CIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFKGT 181
            IH KGF H D+K ANIL F  N+G   +KIADFG++ R     +    A R S   +GT
Sbjct: 121 HIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMRV----DGDLTALRKSVEIRGT 180

Query: 182 PYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVW--EDFSSITYLVTKLVSDMETPK 241
           P YM+PE +    + SA D+W+LGC +++M S +  W  ++ S    L+ ++    E PK
Sbjct: 181 PLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPK 240

Query: 242 IPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKETRTRKKKNKFVVSVQTRE 301
           IPE LSE+GKDF+ +CF + P +RWTA+MLL H ++  D ++      +  FVV V+  +
Sbjct: 241 IPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDH----RENFVVKVKDED 300

Query: 302 EFVNHIKTFMEL-NYHSISLKAGVLYNS 322
           + +   K   E  ++ S +L +   ++S
Sbjct: 301 KVLMSPKCPFEFDDWDSFTLDSNPSFDS 316

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BLAST of Chy12G206540 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.5e-37
Identity = 97/277 (35.02%), Postives = 151/277 (54.51%), Query Frame = 0


Query: 4   WEPVKALGQGSCASVFLARSTMDPLDYFAVKVARL--------RNKSSLSWEKNILKHFI 63
           W+  + LG GS   V+L  ++ +  +  A+K   L         +   L  E ++L   +
Sbjct: 400 WKKGRLLGMGSFGHVYLGFNS-ESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSR-L 459

Query: 64  GCAEIVQCLGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILKGV 123
               IVQ  GSE     D  LY + LEY +GG++  L+Q+  +  E+ ++ Y + IL G+
Sbjct: 460 RHQNIVQYYGSETV---DDKLY-IYLEYVSGGSIYKLLQEYGQFGENAIRNYTQQILSGL 519

Query: 124 SCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFKG 183
           + +H K  VH D+K ANIL  P       +K+ADFG++     KH  A+        FKG
Sbjct: 520 AYLHAKNTVHRDIKGANILVDPHG----RVKVADFGMA-----KHITAQSGPLS---FKG 579

Query: 184 TPYYMSPESI-TSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETPK 243
           +PY+M+PE I  S   N A+DIWSLGC +++M + +P W  +  +  +  K+ +  E P 
Sbjct: 580 SPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMF-KIGNSKELPD 639

Query: 244 IPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLR 272
           IP+ LSE+GKDF+++C  R P  R TA  LL+H ++R
Sbjct: 640 IPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFVR 657

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BLAST of Chy12G206540 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-35
Identity = 88/277 (31.77%), Postives = 148/277 (53.43%), Query Frame = 0


Query: 2   VNWEPVKALGQGSCASVFLARSTMDPLDYFAVKVARLRNKSSLSWEKNILKHFIGCAEIV 61
           + W+  + LG+G   SV+L  +  D  + FAVK   + + +S    KN++  F    E++
Sbjct: 168 IKWQKGQILGRGGYGSVYLGLN-KDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVM 227

Query: 62  QCLGSE-----VTLNGDHLLYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILKGVS 121
           + L  +     +  + D    ++ LEY  GG+++ L+ +    SE+ +K Y K IL+G+S
Sbjct: 228 RSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGLS 287

Query: 122 CIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFK-- 181
            +H    +H D+K ANIL     + K  +K++DFG S          K  S     FK  
Sbjct: 288 FLHANSIIHRDIKGANILI----DTKGIVKLSDFGCS----------KSFSGIVSQFKSM 347

Query: 182 -GTPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETP 241
            GTPY+M+PE I       + DIWSLGC++++M + +P W + + +  ++  + S    P
Sbjct: 348 QGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIP 407

Query: 242 KIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYL 271
            IP  +S++  DF+  CF R PK+R  A+ LL+HP++
Sbjct: 408 NIPSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFI 429

[Close]

BLAST of Chy12G206540 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.8e-35
Identity = 97/290 (33.45%), Postives = 155/290 (53.45%), Query Frame = 0


Query: 4   WEPVKALGQGSCASVFLARSTMDPLDYFAVKVARLRNK--------SSLSWEKNILKHFI 63
           W+  K LG G+   V+L  ++ +     A+K  ++ +           L+ E N+L    
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNS-EKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLC 273

Query: 64  GCAEIVQCLGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILKGV 123
               IVQ  GSE++        ++ LEY +GG++  L++     +E  ++ Y + IL G+
Sbjct: 274 H-PNIVQYYGSELS----EETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGL 333

Query: 124 SCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFKG 183
           + +H +  VH D+K ANIL  P  NG  E+K+ADFG++     KH  A         FKG
Sbjct: 334 AYLHGRNTVHRDIKGANILVDP--NG--EIKLADFGMA-----KHVTAFSTMLS---FKG 393

Query: 184 TPYYMSPESITSK-VFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETPK 243
           +PY+M+PE + S+  +  A+DIWSLGC +++M + +P W  F  +   + K+ +  +TP+
Sbjct: 394 SPYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVA-AIFKIGNSKDTPE 453

Query: 244 IPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKETRTRKKKN 285
           IP+ LS   K+FI+ C  R P  R TA  LLEHP+LR   +   T   K+
Sbjct: 454 IPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVASTSLPKD 484

[Close]

BLAST of Chy12G206540 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.4e-34
Identity = 94/277 (33.94%), Postives = 142/277 (51.26%), Query Frame = 0


Query: 2   VNWEPVKALGQGSCASVFLARSTMDPLDYFAVKVARLRNKSSLSWEKNILKHFIGCAEIV 61
           + W   + LG+GS A+V+ A       +  AVK + +     L  E  IL   +    ++
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSD-EILAVKSSEVHRSEFLQREAKILSS-LSSPYVI 60

Query: 62  QCLGSEVTLNGDH-LLYNLKLEYAAGGTLAD-LIQQRKKLSEDEVKEYLKMILKGVSCIH 121
              GSE     +  ++YNL +EYA  GTL D   +   ++ E  V +Y + ILKG+  IH
Sbjct: 61  GYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIH 120

Query: 122 RKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFKGTPYY 181
            KG VH D+K +N++     + K E KIADFG ++R     E             GTP +
Sbjct: 121 SKGIVHCDVKGSNVVI----SEKGEAKIADFGCAKRVDPVFE---------SPVMGTPAF 180

Query: 182 MSPESITSKVFNSALDIWSLGCILIQMVSEEPVW------EDFSSITYLVTKLVSDMETP 241
           M+PE    +      DIW++GC +I+MV+  P W      ED  S+ Y   ++    ETP
Sbjct: 181 MAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLY---RVGYSSETP 240

Query: 242 KIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYL 271
           ++P  L+E+ KDF+++C  R   +RWTA  LL HP+L
Sbjct: 241 ELPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFL 259

[Close]

BLAST of Chy12G206540 vs. ExPASy TrEMBL
Match: A0A6J1D9A6 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111018431 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 1.3e-89
Identity = 171/278 (61.51%), Postives = 211/278 (75.90%), Query Frame = 0


Query: 1   MVNWEPVKALGQGSCASVFLARSTMDPLDYFAVKVARLRNKSSLSWEKNILKHFIGCAEI 60
           M NW  V  LG+GS   VFLA+ T   L YFAVKVA L++ SSL  EK ILKHF+G  EI
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI 60

Query: 61  VQCLGSEVTLNGDHL---LYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILKGVSC 120
           VQC G E+   GD      Y+L LEYAAGGTLADLI+++KKLSE+ VKEYLKMIL+G+SC
Sbjct: 61  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC 120

Query: 121 IHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFKGTP 180
           IH+ GFVH DLKPANILAFPQ +GKM+LKIADFG+SRRC +K E           F+GTP
Sbjct: 121 IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRE-----CNFKLKFRGTP 180

Query: 181 YYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETPKIPE 240
            YMSPESI  +V N+ LDIWSLGCIL++MV+ +  W+D   +  L+ KL  +METPKIPE
Sbjct: 181 MYMSPESIYGEV-NTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPE 240

Query: 241 ELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQK 276
           ELS++GKDF+++CFDR PK+RWTADMLL+HPYL ++++
Sbjct: 241 ELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKE 272

[Close]

BLAST of Chy12G206540 vs. ExPASy TrEMBL
Match: A0A0A0K835 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407720 PE=4 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 3.0e-86
Identity = 179/298 (60.07%), Postives = 215/298 (72.15%), Query Frame = 0


Query: 3   NWEPVKALGQGSCASVFLARS-TMDPLD---YFAVKVARLR-NKSSLSWEKNILKHFIGC 62
           +W  VKALG+GSC  V LA+  T +  D   YFAVK A LR N SSL WE+++LKHF  C
Sbjct: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63

Query: 63  AEIVQCLGSEVTLNGDHL----LYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILK 122
            EIVQ LGSEVT  GD L    LYNLKLEYAAGGTLADLI+QR KL EDEVK+YL+MILK
Sbjct: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMILK 123

Query: 123 GVSCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLF 182
           G+SCIHRKGFVH DLKP NILAFPQ++GKM+LKIADFG++ R  ++ E  ++  R SK +
Sbjct: 124 GLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQE-DRGSKYY 183

Query: 183 KG------TPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLV 242
            G      T  YMSPESI     N   DIWSLGC L+QMVS E VW D  S   L+TKL+
Sbjct: 184 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 243

Query: 243 SDMETPKIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKETRTRKKKNK 286
              E P IPEELS++GKDF+++CF R  +QRWTADMLL+HPYL ++ K+T+   +K K
Sbjct: 244 ISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 300

[Close]

BLAST of Chy12G206540 vs. ExPASy TrEMBL
Match: A0A6J1DHA9 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111020049 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 3.7e-84
Identity = 158/274 (57.66%), Postives = 199/274 (72.63%), Query Frame = 0


Query: 4   WEPVKALGQGSCASVFLARSTMDPLDYFAVKVARLRNKSSLSWEKNILKHFIGCAEIVQC 63
           W  V  LG+GS   VFLA+ TM P+ Y AVK A +   SSL  EK+IL+HF+GC+EI+QC
Sbjct: 15  WTRVMLLGEGSQGKVFLAKPTMGPIYYVAVKAAFITKSSSLIIEKDILQHFVGCSEIIQC 74

Query: 64  LGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILKGVSCIHRKGF 123
           +G E+TL    L YNL LEYAAGGTL +LI+++K L E+EVKEYLKMIL+G+SCIH KGF
Sbjct: 75  IGDEITLEESSLCYNLILEYAAGGTLENLIKKQKMLPENEVKEYLKMILRGLSCIHDKGF 134

Query: 124 VHADLKPANILAFPQNNGKMELKIADFGISRRC-GEKHEVAKD--ASRCSKLFKGTPYYM 183
           VH DLKP NILAFPQ++GKM+LKIADFG+S+RC  + HEV  D         FKGTP YM
Sbjct: 135 VHGDLKPTNILAFPQSDGKMKLKIADFGLSKRCVHQDHEVVLDTVTPTSKPKFKGTPIYM 194

Query: 184 SPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETP-KIPEEL 243
           SPES+     +   DIWSLGCIL++M++ + VWE   +    + KLV +ME P  IPEEL
Sbjct: 195 SPESVAFGSISPPCDIWSLGCILVEMITGKEVWESCETSGQFIKKLVYEMELPADIPEEL 254

Query: 244 SEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKD 274
           S +G+DF+  CFD  PK+RWTA MLL+HPYL ++
Sbjct: 255 SREGRDFLASCFDHDPKRRWTAHMLLQHPYLTQE 288

[Close]

BLAST of Chy12G206540 vs. ExPASy TrEMBL
Match: A0A5A7TZP4 (Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001750 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 1.2e-82
Identity = 172/294 (58.50%), Postives = 209/294 (71.09%), Query Frame = 0


Query: 1   MVNWEPVKALGQGSCASVFLARS-TMDPLD---YFAVKVARLR-NKSSLSWEKNILKHFI 60
           M +W  +K LG+GSC  V LA+  T +  D   YFAVK A LR N +SL WE+++LKH  
Sbjct: 1   MEDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLT 60

Query: 61  GCAEIVQCLGSEVTLNGDHL----LYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMI 120
            C EIVQ LGSEVT  GD L    LYNLKLEYAAGGTLADLI+QR KL EDEVK+YL+MI
Sbjct: 61  DCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMI 120

Query: 121 LKGVSCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRC--GEKHEVAKDASRC 180
           LKG+SCIHRKGFVH DLKP NILAFPQ++GKM+LKIADFG++ R   GE  +  K     
Sbjct: 121 LKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERFKHGEDDQEDKGCEHY 180

Query: 181 SKL-FKGTPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVS 240
             L  + T  YMSPESI  K  +   DIWSLGC+L+QMVS E VW D  +   L+TKL++
Sbjct: 181 GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLLN 240

Query: 241 DMETPKIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKETRTRKK 283
             E P IP+ELS++G DF+++CF R  KQRWTADMLL+HPYL +  K+T   +K
Sbjct: 241 SKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNKDTSDDEK 294

[Close]

BLAST of Chy12G206540 vs. ExPASy TrEMBL
Match: A0A0A0KAC2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378450 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 3.2e-80
Identity = 161/288 (55.90%), Postives = 202/288 (70.14%), Query Frame = 0


Query: 1   MVNWEPVKALGQGSCASVFLARSTMDPLD------YFAVKVARLRNKSSLSWEKNILKHF 60
           M NW PVK LGQGS A V LA+ ++          YFA+K+  L++ SSL WE+ +LK F
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSIRKCSDNNLPYYFALKIYPLQHNSSLLWEEQVLKQF 60

Query: 61  IGCAEIVQCLGSEVTLNG----DHLLYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKM 120
            GC EIVQ  GSE+T  G    D   Y LKLEYAAGGTL DLI+QR KL EDEVK+YL+M
Sbjct: 61  KGCPEIVQYFGSEITRGGSFCNDKDFYTLKLEYAAGGTLDDLIKQRDKLPEDEVKDYLRM 120

Query: 121 ILKGVSCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVA--KDASR 180
           ILKG+SCIH KGFVH DLKP NILAFPQ++GKM+LKIADFG + RC  + +    K    
Sbjct: 121 ILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERCKYRDDNGQHKRYGY 180

Query: 181 CSKL-FKGTPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLV 240
           CS L FKG+P YMSPESI     + A DIWSLGCIL++M+S + VW+ ++    L+ +++
Sbjct: 181 CSSLKFKGSPRYMSPESIIFNEVDDAHDIWSLGCILVKMISGKCVWDGYTDSKQLMIEVL 240

Query: 241 SDMETPKIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQK 276
            +     IP ELSE+GKDFI++CF R  KQRWTADMLL+HPYL ++ +
Sbjct: 241 DNKIMATIPGELSEQGKDFIRKCFIRSYKQRWTADMLLQHPYLNQENE 288

[Close]

BLAST of Chy12G206540 vs. NCBI nr
Match: XP_022150204.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 337 bits (864), Expect = 4.54e-112
Identity = 174/278 (62.59%), Postives = 213/278 (76.62%), Query Frame = 0


Query: 1   MVNWEPVKALGQGSCASVFLARSTMDPLDYFAVKVARLRNKSSLSWEKNILKHFIGCAEI 60
           M NW  V  LG+GS   VFLA+ T   L YFAVKVA L++ SSL  EK ILKHF+G  EI
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI 60

Query: 61  VQCLGSEVTLNGDHL---LYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILKGVSC 120
           VQC G E+   GD      Y+L LEYAAGGTLADLI+++KKLSE+ VKEYLKMIL+G+SC
Sbjct: 61  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC 120

Query: 121 IHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCS-KL-FKG 180
           IH+ GFVH DLKPANILAFPQ +GKM+LKIADFG+SRRC +K E       C+ KL F+G
Sbjct: 121 IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRE-------CNFKLKFRG 180

Query: 181 TPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETPKI 240
           TP YMSPESI  +V N+ LDIWSLGCIL++MV+ +  W+D   +  L+ KL  +METPKI
Sbjct: 181 TPMYMSPESIYGEV-NTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKI 240

Query: 241 PEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKD 273
           PEELS++GKDF+++CFDR PK+RWTADMLL+HPYL ++
Sbjct: 241 PEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQE 270

[Close]

BLAST of Chy12G206540 vs. NCBI nr
Match: XP_038896325.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 335 bits (859), Expect = 8.48e-111
Identity = 172/285 (60.35%), Postives = 210/285 (73.68%), Query Frame = 0


Query: 1   MVNWEPVKALGQGSCASVFLARS-TMDPLD---YFAVKVARLRNKSSLSWEKNILKHFIG 60
           M NW  VK +G+GS   V LA+  T +  D   YFAVK+  L+ KSSL WE+ +LKHF  
Sbjct: 1   MENWVLVKIIGRGSYGVVCLAKQMTKEKSDRHYYFAVKLTSLQRKSSLLWEEQVLKHFKD 60

Query: 61  CAEIVQCLGSEVTLNG---DHLLYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILK 120
           C EIVQCLGSE+T      D +LYNLKLEYA GGTLADLI+QRKKL EDEVK+YL+MILK
Sbjct: 61  CPEIVQCLGSEITFGDIPDDMVLYNLKLEYAPGGTLADLIKQRKKLPEDEVKDYLRMILK 120

Query: 121 GVSCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLF 180
           G+SCIHR+GFVH DLKPAN+L FPQ++GKM+LKIADFG++ RC  KH V        + F
Sbjct: 121 GLSCIHRRGFVHVDLKPANVLVFPQSDGKMKLKIADFGLAERC--KHIVDDQDRGYLRKF 180

Query: 181 KGTPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETP 240
           KGTP YMSPESI     N   DIWSLGCIL++MVS + VW+D      L+T+L++D   P
Sbjct: 181 KGTPKYMSPESIFFNEVNGTHDIWSLGCILVKMVSGKSVWDDCRGSEQLMTRLLNDKNIP 240

Query: 241 KIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKETR 278
            IP+ELS++GKDFIKRCF R  KQRWTADMLL+HPYL ++ + T+
Sbjct: 241 TIPKELSKQGKDFIKRCFIRNYKQRWTADMLLQHPYLNEENEATK 283

[Close]

BLAST of Chy12G206540 vs. NCBI nr
Match: XP_004136151.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 hypothetical protein Csa_015671 [Cucumis sativus])

HSP 1 Score: 325 bits (833), Expect = 1.43e-106
Identity = 177/291 (60.82%), Postives = 212/291 (72.85%), Query Frame = 0


Query: 3   NWEPVKALGQGSCASVFLARS-TMDPLD---YFAVKVARLR-NKSSLSWEKNILKHFIGC 62
           +W  VKALG+GSC  V LA+  T +  D   YFAVK A LR N SSL WE+++LKHF  C
Sbjct: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63

Query: 63  AEIVQCLGSEVTLNGDHL----LYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILK 122
            EIVQ LGSEVT  GD L    LYNLKLEYAAGGTLADLI+QR KL EDEVK+YL+MILK
Sbjct: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMILK 123

Query: 123 GVSCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLF 182
           G+SCIHRKGFVH DLKP NILAFPQ++GKM+LKIADFG++ R  ++ E  ++  R SK +
Sbjct: 124 GLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQE-DRGSKYY 183

Query: 183 KG------TPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLV 242
            G      T  YMSPESI     N   DIWSLGC L+QMVS E VW D  S   L+TKL+
Sbjct: 184 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 243

Query: 243 SDMETPKIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKETR 278
              E P IPEELS++GKDF+++CF R  +QRWTADMLL+HPYL ++ K+T+
Sbjct: 244 ISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTK 293

[Close]

BLAST of Chy12G206540 vs. NCBI nr
Match: XP_022152301.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 319 bits (818), Expect = 3.17e-105
Identity = 160/272 (58.82%), Postives = 200/272 (73.53%), Query Frame = 0


Query: 3   NWEPVKALGQGSCASVFLARSTMDPLDYFAVKVARLRNKSSLSWEKNILKHFIGCAEIVQ 62
            W  V  LG+GS   VFLA+ TM P+ Y AVK A +   SSL  EK+IL+HF+GC+EI+Q
Sbjct: 14  TWTRVMLLGEGSQGKVFLAKPTMGPIYYVAVKAAFITKSSSLIIEKDILQHFVGCSEIIQ 73

Query: 63  CLGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMILKGVSCIHRKG 122
           C+G E+TL    L YNL LEYAAGGTL +LI+++K L E+EVKEYLKMIL+G+SCIH KG
Sbjct: 74  CIGDEITLEESSLCYNLILEYAAGGTLENLIKKQKMLPENEVKEYLKMILRGLSCIHDKG 133

Query: 123 FVHADLKPANILAFPQNNGKMELKIADFGISRRC-GEKHEVAKDA-SRCSK-LFKGTPYY 182
           FVH DLKP NILAFPQ++GKM+LKIADFG+S+RC  + HEV  D  +  SK  FKGTP Y
Sbjct: 134 FVHGDLKPTNILAFPQSDGKMKLKIADFGLSKRCVHQDHEVVLDTVTPTSKPKFKGTPIY 193

Query: 183 MSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETPK-IPEE 242
           MSPES+     +   DIWSLGCIL++M++ + VWE   +    + KLV +ME P  IPEE
Sbjct: 194 MSPESVAFGSISPPCDIWSLGCILVEMITGKEVWESCETSGQFIKKLVYEMELPADIPEE 253

Query: 243 LSEKGKDFIKRCFDRGPKQRWTADMLLEHPYL 270
           LS +G+DF+  CFD  PK+RWTA MLL+HPYL
Sbjct: 254 LSREGRDFLASCFDHDPKRRWTAHMLLQHPYL 285

[Close]

BLAST of Chy12G206540 vs. NCBI nr
Match: KAA0046699.1 (serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa] >TYK18235.1 serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa])

HSP 1 Score: 314 bits (805), Expect = 2.03e-102
Identity = 171/289 (59.17%), Postives = 207/289 (71.63%), Query Frame = 0


Query: 1   MVNWEPVKALGQGSCASVFLARS-TMDPLD---YFAVKVARLR-NKSSLSWEKNILKHFI 60
           M +W  +K LG+GSC  V LA+  T +  D   YFAVK A LR N +SL WE+++LKH  
Sbjct: 1   MEDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLT 60

Query: 61  GCAEIVQCLGSEVTLNGDHL----LYNLKLEYAAGGTLADLIQQRKKLSEDEVKEYLKMI 120
            C EIVQ LGSEVT  GD L    LYNLKLEYAAGGTLADLI+QR KL EDEVK+YL+MI
Sbjct: 61  DCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMI 120

Query: 121 LKGVSCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRC--GEKHEVAKDASRC 180
           LKG+SCIHRKGFVH DLKP NILAFPQ++GKM+LKIADFG++ R   GE  +  K     
Sbjct: 121 LKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERFKHGEDDQEDKGCEHY 180

Query: 181 SKL-FKGTPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVS 240
             L  + T  YMSPESI  K  +   DIWSLGC+L+QMVS E VW D  +   L+TKL++
Sbjct: 181 GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLLN 240

Query: 241 DMETPKIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKET 277
             E P IP+ELS++G DF+++CF R  KQRWTADMLL+HPYL +  K+T
Sbjct: 241 SKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNKDT 289

[Close]

BLAST of Chy12G206540 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 191.8 bits (486), Expect = 8.5e-49
Identity = 107/283 (37.81%), Postives = 169/283 (59.72%), Query Frame = 0


Query: 2   VNWEPVKALGQGSCASVFLARSTMDPLDYF----AVKVARLRNKSSLSWEKNILKHFIGC 61
           + W   + +G GS ++V LA ++      F    AVK + +   ++L  E+++L     C
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 62  AEIVQCLGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQR-KKLSEDEVKEYLKMILKGVS 121
           +EIV+C G   T+     +YNL LEYA+GG+LAD I+   + L E EV+ + + I+KG+ 
Sbjct: 61  SEIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGLC 120

Query: 122 CIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFKGT 181
            IH  GF H D+K  N+L F    G  ++KI+DFG+++R        +    C ++ +GT
Sbjct: 121 HIHGNGFTHCDIKLENVLVF----GDGDVKISDFGLAKR--------RSGEVCVEI-RGT 180

Query: 182 PYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVW--ED--FSSITYLVTKLVSDMET 241
           P YM+PES+    F S  DIW+LGC +++M S +  W  ED   +++  L+ ++ S  E 
Sbjct: 181 PLYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEV 240

Query: 242 PKIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQK 276
           P+IP ELSE+GKDF+ +CF +   +RWTA+MLL+HP+L  D +
Sbjct: 241 PRIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDE 270

[Close]

BLAST of Chy12G206540 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 185.3 bits (469), Expect = 8.0e-47
Identity = 113/328 (34.45%), Postives = 184/328 (56.10%), Query Frame = 0


Query: 2   VNWEPVKALGQGSCASVFLARSTMDPLDY---FAVKVARLRNKSSLSWEKNILKHFIGCA 61
           + W   + +G G+ ++V  A  + +  D+    AVK       +SLS EK++L     C 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 62  EIVQCLGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQ--RKKLSEDEVKEYLKMILKGVS 121
           EI++C G + T+     ++NL LEYA+ G+LA  +++   + L E  V+ +   +L+G+ 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 122 CIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFKGT 181
            IH KGF H D+K ANIL F  N+G   +KIADFG++ R     +    A R S   +GT
Sbjct: 121 HIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMRV----DGDLTALRKSVEIRGT 180

Query: 182 PYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVW--EDFSSITYLVTKLVSDMETPK 241
           P YM+PE +    + SA D+W+LGC +++M S +  W  ++ S    L+ ++    E PK
Sbjct: 181 PLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPK 240

Query: 242 IPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKETRTRKKKNKFVVSVQTRE 301
           IPE LSE+GKDF+ +CF + P +RWTA+MLL H ++  D ++      +  FVV V+  +
Sbjct: 241 IPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDH----RENFVVKVKDED 300

Query: 302 EFVNHIKTFMEL-NYHSISLKAGVLYNS 322
           + +   K   E  ++ S +L +   ++S
Sbjct: 301 KVLMSPKCPFEFDDWDSFTLDSNPSFDS 316

[Close]

BLAST of Chy12G206540 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 184.5 bits (467), Expect = 1.4e-46
Identity = 109/302 (36.09%), Postives = 176/302 (58.28%), Query Frame = 0


Query: 2   VNWEPVKALGQGSCASVFLA-RSTMDPLDY---FAVKVARLRNKSSLSWEKNILKHF-IG 61
           + W   + +G G+ ++V LA RS  D  ++    AVK A     +SL+ EK++L +    
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDD 60

Query: 62  CAEIVQCLGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQ--RKKLSEDEVKEYLKMILKG 121
           C EIV+C G + T+     ++NL LEYA+ G+L   +++   + + E  V+ +   +L+G
Sbjct: 61  CNEIVRCFGEDRTVENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRG 120

Query: 122 VSCIHRKGFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFK 181
           +  IH  GF H DLK  NIL F    G   +KIADFG+++R G+       A       +
Sbjct: 121 LRHIHANGFAHCDLKLGNILLF----GDGAVKIADFGLAKRIGD-----LTALNYGVQIR 180

Query: 182 GTPYYMSPESITSKVFNSALDIWSLGCILIQMVSEEPVW--EDFSSITYLVTKLVSDMET 241
           GTP YM+PES+    + S  D+W+LGC++++M S +  W  ++ S+   L+ ++    E 
Sbjct: 181 GTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEV 240

Query: 242 PKIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKETRTRKKKNKFVVSVQT 295
           P IPEELSE+G+DF+ +CF + PK+RWTA+MLL HP++  D  +     K+  FVV+++T
Sbjct: 241 PMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVD-VDHDVLVKEEDFVVNMKT 292

[Close]

BLAST of Chy12G206540 vs. TAIR 10
Match: AT2G41930.1 (Protein kinase superfamily protein )

HSP 1 Score: 179.9 bits (455), Expect = 3.3e-45
Identity = 112/284 (39.44%), Postives = 159/284 (55.99%), Query Frame = 0


Query: 5   EPVKALGQGSCASV--FLARSTMDPLDYFAVKVARLRNKSSLSWEKNILKHFIGCAEIVQ 64
           E VK LG+G+  SV  F  +     L Y AVK+    N  S+  E  IL    GC  IVQ
Sbjct: 6   EFVKVLGKGTYGSVELFSHKQNDGSLLYNAVKIMDSENYGSIDQEFRILSELRGCPCIVQ 65

Query: 65  CLGSEVTLNGD---HLLYNLKLEYAAGGTLADLIQQ-RKKLSEDEVKEYLKMILKGVSCI 124
             G+ +    D     +Y + +EYAA GTL + I++ R KLS+  +K++ +MIL+G+  I
Sbjct: 66  LCGNSLVQGIDCNGKKVYMMSMEYAAAGTLTNFIKRNRTKLSDSVIKDFTRMILQGLVSI 125

Query: 125 HRKGFVHADLKPANILAFPQN-----NGKMELKIADFGISRRCGEKHEVAKDASRCSKLF 184
           H  G+VH DLKP NIL FP       N   ELKI+DFGIS R G+K        R  + +
Sbjct: 126 HNHGYVHCDLKPDNILLFPLYDKDTWNCSYELKISDFGISTRAGDK----SGCWRVDEPW 185

Query: 185 KGTPYYMSPESIT-SKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMET 244
            GT  YMSPES++        LD+WSLGCI+++M + +  W  F      V  L+ + + 
Sbjct: 186 VGTSIYMSPESVSDGTTVEKTLDLWSLGCIVLKMYTGKRPWLGFEKD---VKSLLLNQKA 245

Query: 245 PKIPEELSEKGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKE 277
           P+IPE L    + F+++CF R P++R +A  LL HP+L  D+K+
Sbjct: 246 PEIPETLPCDARLFLEKCFSRKPEERGSASELLLHPFLTGDEKK 282

[Close]

BLAST of Chy12G206540 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 177.6 bits (449), Expect = 1.7e-44
Identity = 98/276 (35.51%), Postives = 153/276 (55.43%), Query Frame = 0


Query: 3   NWEPVKALGQGSCASVFLARSTMDPLDYFAVKVARLRNKSSLSWEKNILKHFIGCAEIVQ 62
           NW     +G+GS A+V L  +     D+FAVK A   + + L  E++IL   +    IV+
Sbjct: 5   NWIRGPIIGRGSTATVSLGITNSG--DFFAVKSAEFSSSAFLQREQSILSK-LSSPYIVK 64

Query: 63  CLGSEVTLNGDHLLYNLKLEYAAGGTLADLIQQR-KKLSEDEVKEYLKMILKGVSCIHRK 122
            +GS VT   D L+YNL +EY +GG+L DLI+    KL E  ++ Y + ILKG+  +H +
Sbjct: 65  YIGSNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQ 124

Query: 123 GFVHADLKPANILAFPQNNGKMELKIADFGISRRCGEKHEVAKDASRCSKLFKGTPYYMS 182
           G VH D+K  N++      G    KI D G ++   E   +          F GTP +MS
Sbjct: 125 GIVHCDVKSQNVMI-----GGEIAKIVDLGCAKTVEENENLE---------FSGTPAFMS 184

Query: 183 PESITSKVFNSALDIWSLGCILIQMVSEEPVWEDFSSITYLVTKLVSDMETPKIPEELSE 242
           PE    +  +   D+W+LGC +I+M +    W + + +   + K+    E+P IP  LSE
Sbjct: 185 PEVARGEEQSFPADVWALGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSE 244

Query: 243 KGKDFIKRCFDRGPKQRWTADMLLEHPYLRKDQKET 278
           KG+DF+++C  + PKQRWT + LL+HP+L ++  ++
Sbjct: 245 KGQDFLRKCLRKDPKQRWTVEELLQHPFLDEEDNDS 263

The following BLAST results are available for this feature:

BLAST of Chy12G206540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	1.1e-45	34.45	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
Q9CAD5	2.5e-37	35.02	Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3...	[more]
Q54R82	1.4e-35	31.77	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]
F4HRJ4	1.8e-35	33.45	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]
O80888	3.4e-34	33.94	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Chy12G206540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D9A6	1.3e-89	61.51	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A0A0K835	3.0e-86	60.07	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407...	[more]
A0A6J1DHA9	3.7e-84	57.66	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A5A7TZP4	1.2e-82	58.50	Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A0A0KAC2	3.2e-80	55.90	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378...	[more]

BLAST of Chy12G206540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150204.1	4.54e-112	62.59	mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia]	[more]
XP_038896325.1	8.48e-111	60.35	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_004136151.1	1.43e-106	60.82	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 ...	[more]
XP_022152301.1	3.17e-105	58.82	mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia]	[more]
KAA0046699.1	2.03e-102	59.17	serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa] >TYK18235.1...	[more]

BLAST of Chy12G206540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	8.5e-49	37.81	mitogen-activated protein kinase kinase kinase 21	[more]
AT3G50310.1	8.0e-47	34.45	mitogen-activated protein kinase kinase kinase 20	[more]
AT5G67080.1	1.4e-46	36.09	mitogen-activated protein kinase kinase kinase 19	[more]
AT2G41930.1	3.3e-45	39.44	Protein kinase superfamily protein	[more]
AT5G55090.1	1.7e-44	35.51	mitogen-activated protein kinase kinase kinase 15	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 4..270 e-value: 3.1E-60 score: 216.1
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 6..270 e-value: 2.2E-54 score: 184.6
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 4..270 score: 38.562511
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..319 e-value: 2.4E-63 score: 216.3
None	No IPR available	PIRSR	PIRSR037993-2	PIRSR037993-2	coord: 89..276 e-value: 4.3E-14 score: 50.0
None	No IPR available	PANTHER	PTHR48011:SF51	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 1..302
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 1..302
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G206540.1	Chy12G206540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0004672	protein kinase activity