Chy11G205320 (gene) Cucumber (hystrix) v1

Overview


Name	Chy11G205320
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	RING-type E3 ubiquitin transferase
Location	chrH11: 25559004 .. 25560769 (+)
RNA-Seq Expression	Chy11G205320
Synteny	Chy11G205320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGATATTCACCAAAATCCTTTTCCTCCACCGCACGCACCGACTCCCAGCACTGTGGACTTTCCACCACACTTCCGATGCCCGATTTCCATGGAGCTCATGCAAGATCCTGTCACGATTTCCACCGGAGTTTCCTTTGAAAGAGCCAACATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATTGTCAACTTTGATATCACACCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAAGATCAGTCTTGCTCCTCCACGTCGTCGTTGTTGCCATCGTCCTCAATGGAGTATCATGATGTTGTGGCTGATCTCTTGAAGAACATGGAGTCGTCGCCGTTCAAGGCGAGTTCTTTGAAGAAACTGCGATTGCTCATGGCGGTGGATGATGTAGCTGCTGTTGGAGCGAAACCCGTCTTTGTTCGAGTAAATGGGTTTGAAGTTCTTGTCGGAATAGTCACCCAAGTCGCCATTTGTGAGAGCTCCGATTTTGCTAACTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTGTTTCAAAAACTAATCACCTCTAAAGTTCAAGTTTTTTTTTTCCTTTCTTAATGTTTACATTTAAGTGCGTCTTCAAATATCAACTATAATTTATCGGATTGAAAATATATGTTTTAAGTAGTTGAATCTATAAGTTTTTTTATCAAACATTTACACTTTATAACAGAAGAGTTACGTTTGTAGATACCAATATTAATAATTAGAGATGAGGAATATCATTAAAGATATGAGAGTAGCTACTACTAATTAAATTCAAAAGCTTAAATATTTGGAGGGATAGATAGTGATATATTCATTCTTTTATTCATATTCTTAACACAGGTATTAGCTCAATTCCCTTTTTCCAAAACCGAGAAGCCATTCGAGTTGCTATCAGAACCAGAAGCCATCAAATCAATGGCGATTGTCTTACAAAGAGGAAGCATAGAAGGAAGATTTTATGCCATGGAAATGCTCCAACAAATATCCAAAAAGGGATACGATTGGAACTCTTTGACGAAGCATCAAATCATCGACTTATTCAAATCAATATTGGAGCTTGCATCAGATGAGATAATCAAGAGAGCAAGTGCAATCAAGACAACGGATTCCTCGATAATTACACACTGGTACACTATCATCGTCCACATGCATATTTTCATTTTATTTATAGCTTCACTTTTAGTTGCTAATTAACTGTTTCTTTTCTTAGTCGATATGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCGTCGAAAAAAAGCCGTCTTTTATCGATTGAGGCCGGCGCTGTTTGCGTCATGATCGATCTTCTTCCAGACTCGAACCGATCAAAGTGTGAGAAAATACTACACATACTCAAACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGAGAACATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATCTGTAATTATCATCCAAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATAATGGTGATGGAAGATCTTCAACCAAGGAGAAGGCCATGAAGGTTATGAAGCTTCATGGGAGTTTTTGGAGAAGATTTCCTTGTTTTCCTTATGAGTATAGGGATTATTTGAAGTTGATAAATCAATAG

mRNA sequence

ATGGAGGATATTCACCAAAATCCTTTTCCTCCACCGCACGCACCGACTCCCAGCACTGTGGACTTTCCACCACACTTCCGATGCCCGATTTCCATGGAGCTCATGCAAGATCCTGTCACGATTTCCACCGGAGTTTCCTTTGAAAGAGCCAACATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATTGTCAACTTTGATATCACACCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAAGATCAGTCTTGCTCCTCCACGTCGTCGTTGTTGCCATCGTCCTCAATGGAGTATCATGATGTTGTGGCTGATCTCTTGAAGAACATGGAGTCGTCGCCGTTCAAGGCGAGTTCTTTGAAGAAACTGCGATTGCTCATGGCGGTGGATGATGTAGCTGCTGTTGGAGCGAAACCCGTCTTTGTTCGAGTAAATGGGTTTGAAGTTCTTGTCGGAATAGTCACCCAAGTCGCCATTTGTGAGAGCTCCGATTTTGCTAACTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTAGCTCAATTCCCTTTTTCCAAAACCGAGAAGCCATTCGAGTTGCTATCAGAACCAGAAGCCATCAAATCAATGGCGATTGTCTTACAAAGAGGAAGCATAGAAGGAAGATTTTATGCCATGGAAATGCTCCAACAAATATCCAAAAAGGGATACGATTGGAACTCTTTGACGAAGCATCAAATCATCGACTTATTCAAATCAATATTGGAGCTTGCATCAGATGAGATAATCAAGAGAGCAAGTGCAATCAAGACAACGGATTCCTCGATAATTACACACTGTCGATATGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCGTCGAAAAAAAGCCGTCTTTTATCGATTGAGGCCGGCGCTGTTTGCGTCATGATCGATCTTCTTCCAGACTCGAACCGATCAAAGTGTGAGAAAATACTACACATACTCAAACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGAGAACATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATCTGTAATTATCATCCAAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATAATGGTGATGGAAGATCTTCAACCAAGGAGAAGGCCATGAAGGTTATGAAGCTTCATGGGAGTTTTTGGAGAAGATTTCCTTGTTTTCCTTATGAGTATAGGGATTATTTGAAGTTGATAAATCAATAG

Coding sequence (CDS)

ATGGAGGATATTCACCAAAATCCTTTTCCTCCACCGCACGCACCGACTCCCAGCACTGTGGACTTTCCACCACACTTCCGATGCCCGATTTCCATGGAGCTCATGCAAGATCCTGTCACGATTTCCACCGGAGTTTCCTTTGAAAGAGCCAACATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATTGTCAACTTTGATATCACACCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAAGATCAGTCTTGCTCCTCCACGTCGTCGTTGTTGCCATCGTCCTCAATGGAGTATCATGATGTTGTGGCTGATCTCTTGAAGAACATGGAGTCGTCGCCGTTCAAGGCGAGTTCTTTGAAGAAACTGCGATTGCTCATGGCGGTGGATGATGTAGCTGCTGTTGGAGCGAAACCCGTCTTTGTTCGAGTAAATGGGTTTGAAGTTCTTGTCGGAATAGTCACCCAAGTCGCCATTTGTGAGAGCTCCGATTTTGCTAACTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTAGCTCAATTCCCTTTTTCCAAAACCGAGAAGCCATTCGAGTTGCTATCAGAACCAGAAGCCATCAAATCAATGGCGATTGTCTTACAAAGAGGAAGCATAGAAGGAAGATTTTATGCCATGGAAATGCTCCAACAAATATCCAAAAAGGGATACGATTGGAACTCTTTGACGAAGCATCAAATCATCGACTTATTCAAATCAATATTGGAGCTTGCATCAGATGAGATAATCAAGAGAGCAAGTGCAATCAAGACAACGGATTCCTCGATAATTACACACTGTCGATATGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCGTCGAAAAAAAGCCGTCTTTTATCGATTGAGGCCGGCGCTGTTTGCGTCATGATCGATCTTCTTCCAGACTCGAACCGATCAAAGTGTGAGAAAATACTACACATACTCAAACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGAGAACATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATCTGTAATTATCATCCAAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATAATGGTGATGGAAGATCTTCAACCAAGGAGAAGGCCATGAAGGTTATGAAGCTTCATGGGAGTTTTTGGAGAAGATTTCCTTGTTTTCCTTATGAGTATAGGGATTATTTGAAGTTGATAAATCAATAG

Protein sequence

MEDIHQNPFPPPHAPTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNMESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFEACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ*

Homology

[Close]

BLAST of Chy11G205320 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 7.8e-45
Identity = 130/427 (30.44%), Postives = 211/427 (49.41%), Query Frame = 0


Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 79
           ++ PP F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 80  YTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNMESSPFKASSLKKLRLLMA 139
           +TL+RLI SW   + S        P   +   ++   +  +  S   +   LK+LR +++
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVS 129

Query: 140 VDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFEACEEALGVLAQFPFSKT-- 199
            +       K         E L  IV       S+D  N    +EAL +L     S+T  
Sbjct: 130 EN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVL 189

Query: 200 EKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFKSI 259
           +       +   +KS+  ++QRG  E R YA  +L+ I +      S+T     ++F  +
Sbjct: 190 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEV 249

Query: 260 LELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVC 319
           +++  D I ++A+  K     ++  C +G                  ++R  ++EAG + 
Sbjct: 250 VQILDDRISQKAT--KAAMHILVNICPWG------------------RNRHKAVEAGVIS 309

Query: 320 VMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNTAT 379
           V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S TA+
Sbjct: 310 VIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTAS 369

Query: 380 KIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAMKVMKLHGSFW 439
              +++L  +  +  +  +L EM+  G V KL  +L    G   TKEKA +++KLH   W
Sbjct: 370 DRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCG-GKTKEKAKELLKLHARVW 402

[Close]

BLAST of Chy11G205320 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.4e-40
Identity = 122/450 (27.11%), Postives = 224/450 (49.78%), Query Frame = 0


Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 79
           ++ P  F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 80  YTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNMESSPFKASSLKKLRLLMA 139
           +TL+RLI SW   + S        P   +   ++   + ++  S   +   LK+LR +++
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 140 VD-------DVAAVGAKPVFVRVNGFEVLVGIVTQVA------ICESSDFAN-FEA---- 199
            +       + A V      +  N  +      + ++      +C+S+   N F++    
Sbjct: 125 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 184

Query: 200 CEEALGVLAQFPFSKTEKPFELLSE--PEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 259
            +EAL VL     S+T     L ++     +K++  ++QRG  E R YA  +L+++ +  
Sbjct: 185 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 244

Query: 260 YDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEI 319
                +   +  +LF  ++++  D+I  +A+  ++    ++  C +G             
Sbjct: 245 DPMQIILLER--ELFGEVIQILHDQISHKAT--RSAMQILVITCPWG------------- 304

Query: 320 MESSKKSRLLSIEAGAVCVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHI 379
                ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+    H 
Sbjct: 305 -----RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 364

Query: 380 MGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR 439
             IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L    G 
Sbjct: 365 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG- 424

Query: 440 SSTKEKAMKVMKLHGSFWRRFPCFPYEYRD 445
           + TKEKA +++KLH   WR  PC P    D
Sbjct: 425 NKTKEKAKELLKLHARVWRESPCVPRNLYD 431

[Close]

BLAST of Chy11G205320 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.3e-33
Identity = 124/434 (28.57%), Postives = 202/434 (46.54%), Query Frame = 0


Query: 15  PTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 74
           P    +  P HFRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDF 66

Query: 75  DITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLK------NMESSPFKA 134
            + PN+TL+RLI  W + ++S        P    +   V A L +         S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRA 126

Query: 135 SSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFEACEEALGV 194
           ++L++LR      D   V    +    N  E+L+ I+        S+  + E   E+L +
Sbjct: 127 AALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLAL 186

Query: 195 LAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQIS--KKGYDWNSLT 254
           L   P ++  +   + S+P  ++ +  +L   SIE R  A  +++ +S   K  D     
Sbjct: 187 LVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSI 246

Query: 255 KHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEIMESSKKS 314
            +    +F+ +L+L  + I  R  A+K    ++   C                  S K +
Sbjct: 247 SNS-ESVFEGVLDLLRNPISSR-RALKIGIKTLFALC------------------SVKST 306

Query: 315 RLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK- 374
           R ++I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  
Sbjct: 307 RHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTI 366

Query: 375 MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAMKV 434
           + +S+ AT+     L  +C     ER  EE  G G V +LL ++   +     K+KA K+
Sbjct: 367 LRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLL-LMVQSECTERAKKKAQKL 406

Query: 435 MKLHGSFWRRFPCF 439
           +KL    W  +  F
Sbjct: 427 LKLLRDSWPDYNSF 406

[Close]

BLAST of Chy11G205320 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.5e-32
Identity = 115/441 (26.08%), Postives = 213/441 (48.30%), Query Frame = 0


Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 79
           ++ P +F CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 8   IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 67

Query: 80  NYTLKRLILSWKMKDQS---CSSTSSLLPSSSMEYHDVVADLLKNMESSPFKASSLKKLR 139
           N+ L+RLI  W +++++      ++  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 68  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 127

Query: 140 LLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFEACEEALGVLAQFPFS 199
           +L A+D       + +       + L+  V +    +       +  +E+L +L      
Sbjct: 128 VL-AMDG----NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 187

Query: 200 KTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFK 259
             +    L+     ++S+  VL +     + Y + +L+ +++  Y  + + +    ++FK
Sbjct: 188 SNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFK 247

Query: 260 SILELASDEI----------------IKRASAIKTTDSSIITHCRYGTLFSSALELLIEI 319
            I+    D +                  R S  KT  S +          ++AL +L+E 
Sbjct: 248 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE- 307

Query: 320 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSK--CEKILHILKLLSECAEGRSAMGEHIMG 379
             S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+ +  H  G
Sbjct: 308 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 367

Query: 380 IATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSS 438
           IA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L    G  +
Sbjct: 368 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG-LN 427

[Close]

BLAST of Chy11G205320 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.5e-27
Identity = 121/428 (28.27%), Postives = 197/428 (46.03%), Query Frame = 0


Query: 15  PTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 74
           P    +  P HFRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDF 66

Query: 75  DITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDV------VADLLKNMESSPFKA 134
            + PN+TL+RLI  W + ++S        P    +   V       + +     S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRA 126

Query: 135 SSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFEACEEALGV 194
           +++++LR L    +   V    +    N  E+LV I+   A  E++  ++ E   E+L +
Sbjct: 127 AAIRRLRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSESLAL 186

Query: 195 LAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQI--SKKGYDWNSLT 254
           L     ++TE    + S+P  +  M  +L   SIE R  A  +++ +    K  D   + 
Sbjct: 187 LVLLHMTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLII 246

Query: 255 KHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEIMESSKKS 314
                 +F+ +L+L  + I  R  A+K    +I   C                    K++
Sbjct: 247 SGS-DSIFEGVLDLLKNPISSR-RALKIGIKAIFALCLV------------------KQT 306

Query: 315 RLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK- 374
           R L+I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  
Sbjct: 307 RHLAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTI 366

Query: 375 MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAMKV 433
           + +S+ AT+     L  +C     ER  +E    G V +LL +L   D     K KA  +
Sbjct: 367 LRVSDRATEYAAGALLALCT--AEERCRDEAAAAGLVTQLL-LLVQSDCTERAKRKAQML 402

[Close]

BLAST of Chy11G205320 vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 6.9e-254
Identity = 444/451 (98.45%), Postives = 450/451 (99.78%), Query Frame = 0


Query: 1   MEDIHQNPFPPPHAPTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60
           MEDIHQNPFPPPHAPTP+TVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN
Sbjct: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60

Query: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120
           KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN
Sbjct: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120

Query: 121 MESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFE 180
           +ESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVL+GIVTQVAICESSDFANFE
Sbjct: 121 IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE 180

Query: 181 ACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY 240
           ACEEALGVL+QFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY
Sbjct: 181 ACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY 240

Query: 241 DWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEIM 300
           DWNSLTKHQIIDLFKSILELASDEIIK+ASAIKTTDSSI+THCRYGTLFSSALELLIEIM
Sbjct: 241 DWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIM 300

Query: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360
           ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT
Sbjct: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360

Query: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK 420
           VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK
Sbjct: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK 420

Query: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           A KVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 AKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]

BLAST of Chy11G205320 vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.3e-236
Identity = 426/454 (93.83%), Postives = 438/454 (96.48%), Query Frame = 0


Query: 1   MEDIHQNPFPPPHAPTP---STVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+PFPP H P+P   ++VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADL 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSST SLLPSSSMEYHDVVADL
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSST-SLLPSSSMEYHDVVADL 120

Query: 121 LKNMESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFA 180
           LKN+ESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFE+LV IVTQVAICE SDFA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180

Query: 181 NFEACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVL+QFPFSKTEKPFELLSEPEAIKSMAI+LQRGSIEGRFYAMEMLQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLI 300
           KGYDWNSLTKHQIIDLFKSILELASDEIIK+ASAIKT DSS IT CRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSST 420
           IATVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGIL NGDG SST
Sbjct: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGIL-NGDGSSST 420

Query: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]

BLAST of Chy11G205320 vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 5.0e-212
Identity = 386/454 (85.02%), Postives = 412/454 (90.75%), Query Frame = 0


Query: 1   MEDIHQNPFPPPH--APTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN  PPP    P P   D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLP-SSSMEYHDVVADL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCSS SS  P   SMEY DVVA L
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSSASSSSPRPPSMEYDDVVA-L 120

Query: 121 LKNMESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFA 180
           LKN+E SPFKASSLKKLRLLMA+DDV AVGAKP+FV+ NGFEVLVG+VTQ+AICESS+FA
Sbjct: 121 LKNIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFA 180

Query: 181 NFEACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVL+QFPFSK EK FELL+ PEAIKSMAIVLQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIK+AS +K TDSSI THCRYGTLFSSALELLI
Sbjct: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMG
Sbjct: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR-SS 420
           IATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEM+GCGTVKKL+GIL NGDGR SS
Sbjct: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGIL-NGDGRSSS 420

Query: 421 TKEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 451
           TKEKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Chy11G205320 vs. ExPASy TrEMBL
Match: A0A5D3DLY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00740 PE=4 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 1.9e-211
Identity = 391/454 (86.12%), Postives = 404/454 (88.99%), Query Frame = 0


Query: 1   MEDIHQNPFPPPHAPTP---STVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+PFPP H P+P   ++VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADL 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSST SLLPSSSMEYHDVVADL
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSST-SLLPSSSMEYHDVVADL 120

Query: 121 LKNMESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFA 180
           LKN+ESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFE+LV IVTQVAICE SDF 
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFT 180

Query: 181 NFEACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVL+QFPFSKTEKPFELLSEPEAIKSMAI+LQRGSIEGRFYAMEMLQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLI 300
           KGYDWNSLTKHQIIDLFKSILELASDEIIK+ASAIKTTDSS IT CRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEG          
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG---------- 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSST 420
                                     +YHPSERVLEEMMGCGTVKKLLGIL NGDG SST
Sbjct: 361 --------------------------SYHPSERVLEEMMGCGTVKKLLGIL-NGDGSSST 415

Query: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           KEKAMKVMKLHGSFWR+FPCFPYEYRDYLKLINQ
Sbjct: 421 KEKAMKVMKLHGSFWRKFPCFPYEYRDYLKLINQ 415

[Close]

BLAST of Chy11G205320 vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 5.1e-209
Identity = 384/452 (84.96%), Postives = 410/452 (90.71%), Query Frame = 0


Query: 1   MEDIHQNPFPPPHAPTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60
           MEDI QN  PPP    P+  D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFTYN
Sbjct: 1   MEDIRQNRLPPPQ--PPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYN 60

Query: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLP-SSSMEYHDVVADLLK 120
           KKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCS  SS  P   S+EY DVVA LLK
Sbjct: 61  KKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSFASSSSPRPPSVEYDDVVA-LLK 120

Query: 121 NMESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANF 180
           N+E SPFKASSLKKLRLLMA+DDVAAV AK +FV+ NGFEVLVG+VTQVAICESS+FANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVL+QFPFSKTEK FELLS+ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240

Query: 241 YDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEI 300
           YD+NSL+KH  IDLFKSILELASDEIIK+AS IKTTDSSI T CRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360
           MESSKK RLL IEAGA+C+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR-SSTK 420
           TVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GIL NGDGR SSTK
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGIL-NGDGRSSSTK 420

Query: 421 EKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 451
           EKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

[Close]

BLAST of Chy11G205320 vs. NCBI nr
Match: XP_004145072.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical protein Csa_004999 [Cucumis sativus])

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 444/451 (98.45%), Postives = 450/451 (99.78%), Query Frame = 0


Query: 1   MEDIHQNPFPPPHAPTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60
           MEDIHQNPFPPPHAPTP+TVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN
Sbjct: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60

Query: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120
           KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN
Sbjct: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120

Query: 121 MESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFE 180
           +ESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVL+GIVTQVAICESSDFANFE
Sbjct: 121 IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE 180

Query: 181 ACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY 240
           ACEEALGVL+QFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY
Sbjct: 181 ACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY 240

Query: 241 DWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEIM 300
           DWNSLTKHQIIDLFKSILELASDEIIK+ASAIKTTDSSI+THCRYGTLFSSALELLIEIM
Sbjct: 241 DWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIM 300

Query: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360
           ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT
Sbjct: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360

Query: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK 420
           VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK
Sbjct: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK 420

Query: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451
           A KVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 AKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]

BLAST of Chy11G205320 vs. NCBI nr
Match: XP_008460073.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 830 bits (2143), Expect = 4.18e-302
Identity = 426/454 (93.83%), Postives = 438/454 (96.48%), Query Frame = 0


Query: 1   MEDIHQNPFPPPHAPTP---STVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+PFPP H P+P   ++VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADL 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK+ SCSSTS LLPSSSMEYHDVVADL
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKE-SCSSTS-LLPSSSMEYHDVVADL 120

Query: 121 LKNMESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFA 180
           LKN+ESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFE+LV IVTQVAICE SDFA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180

Query: 181 NFEACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVL+QFPFSKTEKPFELLSEPEAIKSMAI+LQRGSIEGRFYAMEMLQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLI 300
           KGYDWNSLTKHQIIDLFKSILELASDEIIK+ASAIKT DSS IT CRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSST 420
           IATVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGILN GDG SST
Sbjct: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILN-GDGSSST 420

Query: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451
           KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]

BLAST of Chy11G205320 vs. NCBI nr
Match: XP_038875803.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 812 bits (2097), Expect = 4.11e-295
Identity = 415/453 (91.61%), Postives = 431/453 (95.14%), Query Frame = 0


Query: 1   MEDIHQNPFPPPHAPTPST--VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDIHQN  PPP  P PS   VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLL 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWK K+ S SS+SS  PSSSM+Y DVVADLL
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSS--PSSSMKYDDVVADLL 120

Query: 121 KNMESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFAN 180
           KN+ES+PFKASSLKKLRLLMA+DDVAAVGAKPVFVR NGF+VLVGI+TQVAICES+DFAN
Sbjct: 121 KNIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFAN 180

Query: 181 FEACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVL+QFPFSKTEKPFELLS PEAIKSMAIVLQRGS+EGRFYAMEMLQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKK 240

Query: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIE 300
            YDWNSLTKH IIDLFKSILELASDEIIK+AS IKTT+ SIIT CRYGTLFSSALELLIE
Sbjct: 241 EYDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTK 420
           ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILN GDGRSSTK
Sbjct: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILN-GDGRSSTK 420

Query: 421 EKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451
           EKAMK+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 450

[Close]

BLAST of Chy11G205320 vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 754 bits (1946), Expect = 4.27e-272
Identity = 391/454 (86.12%), Postives = 414/454 (91.19%), Query Frame = 0


Query: 1   MEDIHQNPFPP--PHAPTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN  PP  P  P P+  D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSS-SMEYHDVVADL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCSS SS  P   SMEY DVVA L
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSSASSSSPGPPSMEYDDVVA-L 120

Query: 121 LKNMESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFA 180
           LKN+E SPFKASSLKKLRLLMA+DDVAAVGAKP+FV+ NGFEVLVG+VTQVAICESS+FA
Sbjct: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFA 180

Query: 181 NFEACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVL+QFPFSKTEK FELLS PEAIKSMAIVLQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLI 300
           KGYD+NSLTKH  IDLFKSILELASDEIIK+AS +K TDSSI T CRYGTLFSSALELLI
Sbjct: 241 KGYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMG
Sbjct: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSS- 420
           IATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GILN GDGRSS 
Sbjct: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILN-GDGRSSS 420

Query: 421 TKEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 450
           TKEKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Chy11G205320 vs. NCBI nr
Match: XP_022958929.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 748 bits (1931), Expect = 8.22e-270
Identity = 386/454 (85.02%), Postives = 412/454 (90.75%), Query Frame = 0


Query: 1   MEDIHQNPFPPPHA--PTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN  PPP    P P   D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSS-SMEYHDVVADL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCSS SS  P   SMEY DVVA L
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSSASSSSPRPPSMEYDDVVA-L 120

Query: 121 LKNMESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFA 180
           LKN+E SPFKASSLKKLRLLMA+DDV AVGAKP+FV+ NGFEVLVG+VTQ+AICESS+FA
Sbjct: 121 LKNIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFA 180

Query: 181 NFEACEEALGVLAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVL+QFPFSK EK FELL+ PEAIKSMAIVLQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIK+AS +K TDSSI THCRYGTLFSSALELLI
Sbjct: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMG
Sbjct: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSS- 420
           IATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEM+GCGTVKKL+GILN GDGRSS 
Sbjct: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILN-GDGRSSS 420

Query: 421 TKEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 450
           TKEKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Chy11G205320 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 183.0 bits (463), Expect = 5.5e-46
Identity = 130/427 (30.44%), Postives = 211/427 (49.41%), Query Frame = 0


Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 79
           ++ PP F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 80  YTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNMESSPFKASSLKKLRLLMA 139
           +TL+RLI SW   + S        P   +   ++   +  +  S   +   LK+LR +++
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVS 129

Query: 140 VDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFEACEEALGVLAQFPFSKT-- 199
            +       K         E L  IV       S+D  N    +EAL +L     S+T  
Sbjct: 130 EN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVL 189

Query: 200 EKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFKSI 259
           +       +   +KS+  ++QRG  E R YA  +L+ I +      S+T     ++F  +
Sbjct: 190 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEV 249

Query: 260 LELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVC 319
           +++  D I ++A+  K     ++  C +G                  ++R  ++EAG + 
Sbjct: 250 VQILDDRISQKAT--KAAMHILVNICPWG------------------RNRHKAVEAGVIS 309

Query: 320 VMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNTAT 379
           V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S TA+
Sbjct: 310 VIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTAS 369

Query: 380 KIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAMKVMKLHGSFW 439
              +++L  +  +  +  +L EM+  G V KL  +L    G   TKEKA +++KLH   W
Sbjct: 370 DRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCG-GKTKEKAKELLKLHARVW 402

[Close]

BLAST of Chy11G205320 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 167.2 bits (422), Expect = 3.1e-41
Identity = 122/450 (27.11%), Postives = 224/450 (49.78%), Query Frame = 0


Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 79
           ++ P  F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 80  YTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNMESSPFKASSLKKLRLLMA 139
           +TL+RLI SW   + S        P   +   ++   + ++  S   +   LK+LR +++
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 140 VD-------DVAAVGAKPVFVRVNGFEVLVGIVTQVA------ICESSDFAN-FEA---- 199
            +       + A V      +  N  +      + ++      +C+S+   N F++    
Sbjct: 125 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 184

Query: 200 CEEALGVLAQFPFSKTEKPFELLSE--PEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 259
            +EAL VL     S+T     L ++     +K++  ++QRG  E R YA  +L+++ +  
Sbjct: 185 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 244

Query: 260 YDWNSLTKHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEI 319
                +   +  +LF  ++++  D+I  +A+  ++    ++  C +G             
Sbjct: 245 DPMQIILLER--ELFGEVIQILHDQISHKAT--RSAMQILVITCPWG------------- 304

Query: 320 MESSKKSRLLSIEAGAVCVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHI 379
                ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+    H 
Sbjct: 305 -----RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 364

Query: 380 MGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR 439
             IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L    G 
Sbjct: 365 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG- 424

Query: 440 SSTKEKAMKVMKLHGSFWRRFPCFPYEYRD 445
           + TKEKA +++KLH   WR  PC P    D
Sbjct: 425 NKTKEKAKELLKLHARVWRESPCVPRNLYD 431

[Close]

BLAST of Chy11G205320 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 144.8 bits (364), Expect = 1.7e-34
Identity = 124/434 (28.57%), Postives = 202/434 (46.54%), Query Frame = 0


Query: 15  PTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 74
           P    +  P HFRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDF 66

Query: 75  DITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLK------NMESSPFKA 134
            + PN+TL+RLI  W + ++S        P    +   V A L +         S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRA 126

Query: 135 SSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFEACEEALGV 194
           ++L++LR      D   V    +    N  E+L+ I+        S+  + E   E+L +
Sbjct: 127 AALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLAL 186

Query: 195 LAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQIS--KKGYDWNSLT 254
           L   P ++  +   + S+P  ++ +  +L   SIE R  A  +++ +S   K  D     
Sbjct: 187 LVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSI 246

Query: 255 KHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEIMESSKKS 314
            +    +F+ +L+L  + I  R  A+K    ++   C                  S K +
Sbjct: 247 SNS-ESVFEGVLDLLRNPISSR-RALKIGIKTLFALC------------------SVKST 306

Query: 315 RLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK- 374
           R ++I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  
Sbjct: 307 RHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTI 366

Query: 375 MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAMKV 434
           + +S+ AT+     L  +C     ER  EE  G G V +LL ++   +     K+KA K+
Sbjct: 367 LRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLL-LMVQSECTERAKKKAQKL 406

Query: 435 MKLHGSFWRRFPCF 439
           +KL    W  +  F
Sbjct: 427 LKLLRDSWPDYNSF 406

[Close]

BLAST of Chy11G205320 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-33
Identity = 115/441 (26.08%), Postives = 213/441 (48.30%), Query Frame = 0


Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 79
           ++ P +F CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 22  IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 81

Query: 80  NYTLKRLILSWKMKDQS---CSSTSSLLPSSSMEYHDVVADLLKNMESSPFKASSLKKLR 139
           N+ L+RLI  W +++++      ++  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 82  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 141

Query: 140 LLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFEACEEALGVLAQFPFS 199
           +L A+D       + +       + L+  V +    +       +  +E+L +L      
Sbjct: 142 VL-AMDG----NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 201

Query: 200 KTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFK 259
             +    L+     ++S+  VL +     + Y + +L+ +++  Y  + + +    ++FK
Sbjct: 202 SNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFK 261

Query: 260 SILELASDEI----------------IKRASAIKTTDSSIITHCRYGTLFSSALELLIEI 319
            I+    D +                  R S  KT  S +          ++AL +L+E 
Sbjct: 262 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE- 321

Query: 320 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSK--CEKILHILKLLSECAEGRSAMGEHIMG 379
             S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+ +  H  G
Sbjct: 322 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 381

Query: 380 IATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSS 438
           IA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L    G  +
Sbjct: 382 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG-LN 441

[Close]

BLAST of Chy11G205320 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-28
Identity = 121/428 (28.27%), Postives = 197/428 (46.03%), Query Frame = 0


Query: 15  PTPSTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 74
           P    +  P HFRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDF 66

Query: 75  DITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDV------VADLLKNMESSPFKA 134
            + PN+TL+RLI  W + ++S        P    +   V       + +     S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRA 126

Query: 135 SSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLVGIVTQVAICESSDFANFEACEEALGV 194
           +++++LR L    +   V    +    N  E+LV I+   A  E++  ++ E   E+L +
Sbjct: 127 AAIRRLRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSESLAL 186

Query: 195 LAQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQI--SKKGYDWNSLT 254
           L     ++TE    + S+P  +  M  +L   SIE R  A  +++ +    K  D   + 
Sbjct: 187 LVLLHMTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLII 246

Query: 255 KHQIIDLFKSILELASDEIIKRASAIKTTDSSIITHCRYGTLFSSALELLIEIMESSKKS 314
                 +F+ +L+L  + I  R  A+K    +I   C                    K++
Sbjct: 247 SGS-DSIFEGVLDLLKNPISSR-RALKIGIKAIFALCLV------------------KQT 306

Query: 315 RLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK- 374
           R L+I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  
Sbjct: 307 RHLAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTI 366

Query: 375 MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAMKV 433
           + +S+ AT+     L  +C     ER  +E    G V +LL +L   D     K KA  +
Sbjct: 367 LRVSDRATEYAAGALLALCT--AEERCRDEAAAAGLVTQLL-LLVQSDCTERAKRKAQML 402

The following BLAST results are available for this feature:

BLAST of Chy11G205320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TG3	7.8e-45	30.44	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SVC6	4.4e-40	27.11	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q9LT79	2.3e-33	28.57	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9SF15	1.5e-32	26.08	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9FXA4	1.5e-27	28.27	U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2...	[more]

BLAST of Chy11G205320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KBP6	6.9e-254	98.45	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE...	[more]
A0A1S3CC54	1.3e-236	93.83	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ...	[more]
A0A6J1H6I8	5.0e-212	85.02	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075...	[more]
A0A5D3DLY2	1.9e-211	86.12	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1KWQ8	5.1e-209	84.96	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P...	[more]

BLAST of Chy11G205320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145072.2	0.0	98.45	E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical pro...	[more]
XP_008460073.1	4.18e-302	93.83	PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo]	[more]
XP_038875803.1	4.11e-295	91.61	E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida]	[more]
XP_023549278.1	4.27e-272	86.12	E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo]	[more]
XP_022958929.1	8.22e-270	85.02	E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata]	[more]

BLAST of Chy11G205320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	5.5e-46	30.44	plant U-box 23	[more]
AT3G52450.1	3.1e-41	27.11	plant U-box 22	[more]
AT3G19380.1	1.7e-34	28.57	plant U-box 25	[more]
AT3G11840.1	1.1e-33	26.08	plant U-box 24	[more]
AT1G49780.1	1.0e-28	28.27	plant U-box 26	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 25..89 e-value: 8.6E-24 score: 95.0
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 23..93 e-value: 5.3E-16 score: 58.6
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 21..96 score: 25.123554
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 14..101 e-value: 1.5E-23 score: 84.6
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 102..430 e-value: 6.9E-10 score: 40.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 21..450
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 21..450
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 26..69 e-value: 6.30331E-21 score: 83.437
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 19..92
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 111..414

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy11G205320.1	Chy11G205320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity