Chy11G205290 (gene) Cucumber (hystrix) v1

Overview
NameChy11G205290
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionEnt-kaurenoic acid oxidase 1-like
LocationchrH11: 25539037 .. 25544958 (+)
RNA-Seq ExpressionChy11G205290
SyntenyChy11G205290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACACCGCAGTGCTCCTGGCAGTGATCCCTTTCCTCGTCTGGCTTCTCAAGAATGCAAACCACTTGCTCTACGAAATCGCCCAGTTGGGTTCGAAACGCTTCGCCCTACCGCCCGGCAACCTCGGCTGGCCCTTCATTGGCAATATGTGGTCTTTTCTAAGAGCTTTCAAGTCTCCTTATCCTGATTCTTTCATGGACTCCTTCCTTTCCAGGTCTCTCTCTCTCTTTGTCTCTTTCTTTCTTTCTTTATTCATATGCACCTATGGAGTTTATAAATTTTGCTATACTAATAATACTAGAAATGGTTCTTCTTTTCTTTTTAATAAAAAAAATACGTTTTTGTTTTTATTCCAATAAGACATTGTCGTATCTTAGACGATATCACAATATCAGAATAATAAATTTGAAGCAAATATATATGAAAATTTTAATTGAAAAGTAGCCATAGTAATTTCTTTTAAGTAAATAATAATACTTTAGGTTTGTCCAACTTCGTATATCTTCGTTACTCTCATGGTAGTCATAGGATGGCATATTAATGATCAGTACGAAAAAGTTTTCATTCCTAAATTTTTTTAAATGTGTCAATTTCTAATAGGTCATTATCTATATAAATTTGTCTATACATAGTCAAATAAGTCTACACCACTTATTGAACCATAAATTAAAAGTTCATTAGACTATTATAGTACTTTTAAATGTTTGAGGTATCATATAGGTTTGAGTACCACACCTTTATTATTTCTATGTTCAATGATAATTTTGGTTAAGATTACAATCGAATATGTGAGGGTTTGATCTTTGGGGTATTTAAAAATGTAATGAGTAATGTTTTAACAGTTAAACTTAACATCATGTTAATTAGAGAAAAAAAAGATAAAATATATAGTTTTCTTTTACTAGATTAAGGAATATTATATCATACATTTTCTTTAAGAAATTTAAATGACAAATTTAATTAGTTATACCATATTATTCAATAATAATCTATCGAGTATGAATATGTTTTCGTACAACAAGTGATTTTGGAAAAAACACATACTATAGAAGTTCTATGAGTGGACCCTCTAGTAGTTTTCATGAGATATATTAGTATAGTTTAAGATTAGGACAAATATTTAAATTTAACTATTAGATATGATATGATATGATTTTATTTTATTTTATTTTATTTTATTCAAACTTTTATACAATATTAATTATTTCTTAATCCCACCTTTTAAAAAATCTTTTTCTGTTTTTTGAAATTTTGACCCATCTTTTAATATTTTTTAGATATAAATAATGGTCCATAAAAGAAGATTTAACTTTAACATACTTTTTTTTAATTGAAATTAAAAAATGTAACAGTTTTATTTTGAAATTTCAACTTTATTTCAATTTAGATTTTAAGATTATTTTAAATTAATTAATATAACATTAATACATCAATAATTGAAATTAAAGTGAGTATTTTAATATAAAAAAAATGAGACTAGAAATGTAAACTTTTTGAAACAGGAACAAAATTGAAACAAAACTCAAAATTTTATCGATAAAATTAAAATATTATTCTAGAATAAAGATCAAATAAAAACTAGAAAGATATAAAATACACACATTTGTTAAACCTAGTACTTTATTTATTTAAAAGAAGAAAACCTAGTACTCTATTATATTGAACACTACCACTCCAATGAAAACTCCTTTTTAGGATAGAAAAGAAAGGTCTTTTTTTCAAAAGAAAATGATAAGAACAAAAATTATTTACATTTTAAGACAAAAATTAATATTTAGTTCATTATGTAATTTTTGACCATTTTCTAAGAAAATTAATGTATTGTATTTCTTATAGTCAACTTTAAAAAGGTTTTCATGGAAATGATAAGGATCCTAAATAATATATATATATATATATATATATATATATATATATATATATATTTATTGTGACAGAAACTTTATGCACCTTAGAAAGCCATCTCATTTTAATTCATCTCTATAAGACAAAATTATTAAGAAAAAAGAAAGTTTTCCATAGAAATTACAAAAATAACTCCCTTTAGTAAGAGACATTTTTAAATATAACAAAATAAACGAAAATATTCAAAAATTTTAGATTTTATCAATAACAAATATTGCTAGATATTGATAAGTTTGTATCGGTGTCTCAAATAGTTACTATAATGAACTTTGAATCTGTGTAAAAAGTATCTAGTATACTCACTAGCTTCGTAAAAGAAAACTTTGAATCTTCTTGATATGTTTTAGATTTATGAGGTTTGTAGTTTGAATTCTCAACGGTATCTTTTACTTGTATTATTATAGAAAAACTTTAATAATTTTTAAAACCATATATATTATTAAAAATTACGTGAAAAAAAAAAAGGAATGAAATTATAAAATAAGAGGGAGAAGAAAAATCCATAGTTGTAAAAGTCATTGGAAAATCAGAGCGGCAGTGGCAAAAATACGTCTCTTTATTCAAAACGGTCATTTCGTCTCTACTGCCGTCGCGGTTAGGCCAATCCTACACTTATCTTTTTATTATTTTTATCTAAGTACGTTTTAGATCAGTTATATCGTTACTGCTTACGTGAAACCCATAAGAACCACAAACTTGAGTCATACTTGATCTTGTAAACTTAGCTCAAATTGATATATGGGTTTTAGAAACGCTTTTAATAGTTTAAGTTTAGGGATGAAACTTGTATAATATAATCCCAACCATTAAAAATATAATTGGTAAAACAGTGAAGGACTAAATGAGAGAATAATTAATGATAAGGACCAATGTGAAACTATAGAAAAGAAGAAGAAAAGTGTAAAAGATATATTTAATCGGAGCTATGAGACAAAGCTTTGCTGCGTCGTTTCCGTTGTCGGTTTCGGTTTCGGTTCTCCAAGGGGTATCGTTTCGGGTAAACCTAACTTTGCCATCTCGGCCCAAGAAATATTTGAGAAAACGTTTAATAATATATATCTCTTTTATTGAATTTTTTTAATTATTGTTTATTTATTTATTTATTTATTTGTGTTTGTCGTTTTCTAATAATAAGTGAAACACCTTTTTTCAAATTCGTGGAAAAGATTTGTTGGGATAAATAATAATGACTTATGAATTACTTTTTTTTTGTTTGGTGGGTGTACCCAAGTTTGTGTAAACCACTCTTTTCAATTTGTTCCAAATTTAAAATGGTGTGTTGTGTTATATTGTCTATGCTTTGATGTCTATTTGCATTTGGAAATGTCTGCAAAGTGGTTTTTGTTTTCAATCTCAGTGCTCTAAAGATTTTGATTCTTTTACCTTTTCTTTATTGTATGAACATAATCTTTTGTGTTTCTTGTTATTCCCTTTCTTTTTAACTTTCTCTATTTATATATATCTATATGGTACAGCTCCACTAGTTTTCATGCTTCTTGTTATCTTTTTAGTTTGATGTTTAAAAGGGTTAGTTACAAATTTAGTCTTCAAACTTTTTGGTTTCAAAACTTCAATGAGTGTCTAATATGGTGTTTGAACTTTCAAATCCGTTTCTAGAAAACGTTTTTCACTTTATAAATGTTTATAGTCTAGTTAGATTCTTAAGCTTTCAATTTAGTCTTTAAGAGTTGAGACAATGAACTTTCTCGATGTTAGTACTTTTTTTTTTTACTAAAAACATATTTTAAATCTTACGTTAGTCCTTGCATCCCTTTTGAATTAGGGTATGATGAAGATACTACGTATGGGTCAATCAAGTTTAGAGATGTTTTTGGTAGTACACTCCGAAAAGCAAGTTTAGATAATACCATGAGGATATTTATTTTTGTCATTGAACTTTTGAAATGTTCATGAGGTTCTTTGTTTTACGTTTTTGTCTCAAAACATTTTGACTCATACTTGAAGATGATCAACATAGAGTAAAACAAAAATTTAGTGCAGAAAAGTAACATAACATCAATTCAAAATCTTTGTTTTTTGAGGGACGATTAAAATAAACGGTTAAGTAGAAAAAATCATATAAACGTTTAGAGACTAGAACAGAACAAATTTAAAATATACGTTTGTGTATTTTTTTGTTTTAGGTATGGAAATACAGGAATGTACAAAGCCTTTATGTTTGGAAGTCCAAGTGTGATTGTAACTTCAGCAGAAGCATGTAAAAGAGTTTTGAATGATGATGAAGCTTTTGGCTCAGGGTGGCCTCTTTCCACCATGAAACTCATAGGAGAAAACTCTTTCATTGGCATTTCTAACCAAGAACACAAGCGGCTCCGCCGTATAACTGCTGCTCCGGTCAACGGTTATGACGCTTTGGCCACCTACCTCACTTACATAGAAAAGATTGTGGTTTCTTCCTTGGACAAATGGGGTTATATGGGAGAAATAGAGTTCTTAACAGAGCTCAGAAAGCTTACCTTTAAGATCATTATGTATATATTTCTTAGCTCTGAAAGTGAGACAATAATGGAGGCTTTGGAGAGAGAGTACACGATGCTTAATCTTGGAGTCAGAGCTATGGCCATCAACATTCCTGGTTTTGCTTACTATAAAGCACTCAAGGTCAGGCATATATGCAAATTATTCGGTTTAGTTTATGAACTTTCTAAGTTTTTCTGAGACTAACACTCAAGGTAAAGACTAAACTTGTAACTTCATTACTAGGCACGAAAAAATCTGGAGGCAACATTTGGATCAATTGTGAGAGGGAGAAGGATGGAAAGAGAGAGGAAGGAGAATTCAAAAACAATGAAAAGAGATATGCTGGATGCTTTGCTAGAAGCTGAAGATGAAGATGGAAGAAGGCTAAGTGATGAAGAAATAATTGATGTCTTGATAATGTATTTAAATGCAGGCCATGAATCTTCAGGTCATACTATGATGTGGGCTACCATTTTCTTACAACAACATCCCCATTTTCTTCAAAAGGCCAAGGTAATTAAGTATATATAAATTAAGGCGAATATATATATATAAATATATGATCTAACTTTGAAAAGAAAAAAATAGGCCGAGCAAGAAGAGATGATAAAAAAACGACCTGTGGGGCAGAAGGGTTTAACTCTCAAGGAAGTCCGACAAATGCCCTATCTTTCAAAGGTATATATATATGTTTAATTTGTTACCTTTTATTTCTTTTAAAAAAACTACTCTTAAATTAATTACTAATCAAAACCAAAAGTTGAACTATCAACGTTTATGATAAATGGTGTAAATTTTTGTAGGTGATCGATGAAACACTACGAGTGGTAACATTCTCACTCACTGTCTTTCGAGAGGCAAAGCATGATGTTAAAATTAGTGGTTAGTTCCATTCATTTTCATGTCCAATCTTTCTCTTTATCATATGGTATTATCCATTATTTGTACATATATATAGCTTATAAATTAAGTAATAGCCTTCCCTCTTTTCTTTTCAATGTAGGCTATACCATTCCAAAAGGTTGGAAAGTTCTAGTTTGGTTTAGGAGTGTTCATTTCGATTCCGAAACTTATCCGAACCCGAGAGAATTTAACCCTTCAAGATGGGATGTGAGAACAAACACATATCTACATAATTCACTGCTCTTTCTTTTTTTTTTCAAGCGGATAGACTTGTAATCTCACTAACATACGTAACGTGAACGTTCAGGATTTCACACCAAAAGCAGGAAGCTTTCTCCCATTTGGAGCAGGAAGTAGATTGTGTCCCGGAAACGATCTTGCAAAACTTGAAATCTCAGTCTTTCTCCACTATTTTCTCCTCAATTATAAGTGAGATCGAGCTCTCTTAATAATCAAATTTAATTAATTTTTTTATTCTTTTTTACCTGTTTTCCCGACGAAACAAGGATATATTGAATCGGGTTGTTTTTGTGGTCAGGTTGGAAAGGGTTAATCCAGAAAGTACAGTGAGGTATCTACCACATTCAAGGCCTCAAGATAACTGTTTGGGAAGGATCAAGAGACTTGCTTAG

mRNA sequence

ATGTACACCGCAGTGCTCCTGGCAGTGATCCCTTTCCTCGTCTGGCTTCTCAAGAATGCAAACCACTTGCTCTACGAAATCGCCCAGTTGGGTTCGAAACGCTTCGCCCTACCGCCCGGCAACCTCGGCTGGCCCTTCATTGGCAATATGTGGTCTTTTCTAAGAGCTTTCAAGTCTCCTTATCCTGATTCTTTCATGGACTCCTTCCTTTCCAGGTATGGAAATACAGGAATGTACAAAGCCTTTATGTTTGGAAGTCCAAGTGTGATTGTAACTTCAGCAGAAGCATGTAAAAGAGTTTTGAATGATGATGAAGCTTTTGGCTCAGGGTGGCCTCTTTCCACCATGAAACTCATAGGAGAAAACTCTTTCATTGGCATTTCTAACCAAGAACACAAGCGGCTCCGCCGTATAACTGCTGCTCCGGTCAACGGTTATGACGCTTTGGCCACCTACCTCACTTACATAGAAAAGATTGTGGTTTCTTCCTTGGACAAATGGGGTTATATGGGAGAAATAGAGTTCTTAACAGAGCTCAGAAAGCTTACCTTTAAGATCATTATGTATATATTTCTTAGCTCTGAAAGTGAGACAATAATGGAGGCTTTGGAGAGAGAGTACACGATGCTTAATCTTGGAGTCAGAGCTATGGCCATCAACATTCCTGGTTTTGCTTACTATAAAGCACTCAAGGCACGAAAAAATCTGGAGGCAACATTTGGATCAATTGTGAGAGGGAGAAGGATGGAAAGAGAGAGGAAGGAGAATTCAAAAACAATGAAAAGAGATATGCTGGATGCTTTGCTAGAAGCTGAAGATGAAGATGGAAGAAGGCTAAGTGATGAAGAAATAATTGATGTCTTGATAATGTATTTAAATGCAGGCCATGAATCTTCAGGTCATACTATGATGTGGGCTACCATTTTCTTACAACAACATCCCCATTTTCTTCAAAAGGCCAAGGCCGAGCAAGAAGAGATGATAAAAAAACGACCTGTGGGGCAGAAGGGTTTAACTCTCAAGGAAGTCCGACAAATGCCCTATCTTTCAAAGGTGATCGATGAAACACTACGAGTGGTAACATTCTCACTCACTGTCTTTCGAGAGGCAAAGCATGATGTTAAAATTAGTGGCTATACCATTCCAAAAGGTTGGAAAGTTCTAGTTTGGTTTAGGAGTGTTCATTTCGATTCCGAAACTTATCCGAACCCGAGAGAATTTAACCCTTCAAGATGGGATGATTTCACACCAAAAGCAGGAAGCTTTCTCCCATTTGGAGCAGGAAGTAGATTGTGTCCCGGAAACGATCTTGCAAAACTTGAAATCTCAGTCTTTCTCCACTATTTTCTCCTCAATTATAAGTTGGAAAGGGTTAATCCAGAAAGTACAGTGAGGTATCTACCACATTCAAGGCCTCAAGATAACTGTTTGGGAAGGATCAAGAGACTTGCTTAG

Coding sequence (CDS)

ATGTACACCGCAGTGCTCCTGGCAGTGATCCCTTTCCTCGTCTGGCTTCTCAAGAATGCAAACCACTTGCTCTACGAAATCGCCCAGTTGGGTTCGAAACGCTTCGCCCTACCGCCCGGCAACCTCGGCTGGCCCTTCATTGGCAATATGTGGTCTTTTCTAAGAGCTTTCAAGTCTCCTTATCCTGATTCTTTCATGGACTCCTTCCTTTCCAGGTATGGAAATACAGGAATGTACAAAGCCTTTATGTTTGGAAGTCCAAGTGTGATTGTAACTTCAGCAGAAGCATGTAAAAGAGTTTTGAATGATGATGAAGCTTTTGGCTCAGGGTGGCCTCTTTCCACCATGAAACTCATAGGAGAAAACTCTTTCATTGGCATTTCTAACCAAGAACACAAGCGGCTCCGCCGTATAACTGCTGCTCCGGTCAACGGTTATGACGCTTTGGCCACCTACCTCACTTACATAGAAAAGATTGTGGTTTCTTCCTTGGACAAATGGGGTTATATGGGAGAAATAGAGTTCTTAACAGAGCTCAGAAAGCTTACCTTTAAGATCATTATGTATATATTTCTTAGCTCTGAAAGTGAGACAATAATGGAGGCTTTGGAGAGAGAGTACACGATGCTTAATCTTGGAGTCAGAGCTATGGCCATCAACATTCCTGGTTTTGCTTACTATAAAGCACTCAAGGCACGAAAAAATCTGGAGGCAACATTTGGATCAATTGTGAGAGGGAGAAGGATGGAAAGAGAGAGGAAGGAGAATTCAAAAACAATGAAAAGAGATATGCTGGATGCTTTGCTAGAAGCTGAAGATGAAGATGGAAGAAGGCTAAGTGATGAAGAAATAATTGATGTCTTGATAATGTATTTAAATGCAGGCCATGAATCTTCAGGTCATACTATGATGTGGGCTACCATTTTCTTACAACAACATCCCCATTTTCTTCAAAAGGCCAAGGCCGAGCAAGAAGAGATGATAAAAAAACGACCTGTGGGGCAGAAGGGTTTAACTCTCAAGGAAGTCCGACAAATGCCCTATCTTTCAAAGGTGATCGATGAAACACTACGAGTGGTAACATTCTCACTCACTGTCTTTCGAGAGGCAAAGCATGATGTTAAAATTAGTGGCTATACCATTCCAAAAGGTTGGAAAGTTCTAGTTTGGTTTAGGAGTGTTCATTTCGATTCCGAAACTTATCCGAACCCGAGAGAATTTAACCCTTCAAGATGGGATGATTTCACACCAAAAGCAGGAAGCTTTCTCCCATTTGGAGCAGGAAGTAGATTGTGTCCCGGAAACGATCTTGCAAAACTTGAAATCTCAGTCTTTCTCCACTATTTTCTCCTCAATTATAAGTTGGAAAGGGTTAATCCAGAAAGTACAGTGAGGTATCTACCACATTCAAGGCCTCAAGATAACTGTTTGGGAAGGATCAAGAGACTTGCTTAG

Protein sequence

MYTAVLLAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSPYPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIGENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELRKLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATFGSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVVTFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAGSFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRIKRLA*
Homology
BLAST of Chy11G205290 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.2e-189
Identity = 321/469 (68.44%), Postives = 388/469 (82.73%), Query Frame = 0

Query: 13  LVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSPYPDSFMDSFLSR 72
           L WL++  N  LYE + LG  R  LPPG+LGWPFIGNM SFLRAFK+  PDSF  + + R
Sbjct: 22  LNWLVRKVNVWLYE-SSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKR 81

Query: 73  YGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIGENSFIGISNQEH 132
           YG  G+YKA MFG+PS+IVT+++ C+RVL DD+AF  GWP STM+LIG  SF+GIS +EH
Sbjct: 82  YGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEH 141

Query: 133 KRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELRKLTFKIIMYIFL 192
           KRLRR+TAAPVNG++AL+TY+ YIE+ V++ LDKW  MGE EFLT LRKLTF+IIMYIFL
Sbjct: 142 KRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIFL 201

Query: 193 SSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATFGSIVRGRRMERE 252
           SSESE +M+ALEREYT LN GVRAMA+NIPGFAY++ALKARK L A F SIV  RR   +
Sbjct: 202 SSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERR--NQ 261

Query: 253 RKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQ 312
           RK+N  + K+DMLD LL  +DEDG+ L DEEIIDVL+MYLNAGHESSGHT+MWAT+FLQ+
Sbjct: 262 RKQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQE 321

Query: 313 HPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVVTFSLTVFREAKH 372
           HP  LQ+AKAEQE ++K RP GQKGL+LKE R+M +LS+V+DETLRV+TFSLT FREAK 
Sbjct: 322 HPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKT 381

Query: 373 DVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDD-FTPKAGSFLPFGAGSRL 432
           DV+++GY IPKGWKVL WFR VH D E +P+PR+F+P+RWD+ F PKAG+FLPFGAGS L
Sbjct: 382 DVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHL 441

Query: 433 CPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRI 481
           CPGNDLAKLEIS+FLH+FLL Y+++R NPE  V YLPH+RP DNCL RI
Sbjct: 442 CPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of Chy11G205290 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 647.1 bits (1668), Expect = 1.6e-184
Identity = 313/482 (64.94%), Postives = 390/482 (80.91%), Query Frame = 0

Query: 1   MYTAVLLAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP 60
           M+  +++  +  L W+LK  N  +Y +++LG K+  LPPG+LGWP IGNMWSFLRAFK+ 
Sbjct: 9   MWFPLIILGLFVLKWVLKRVNVWIY-VSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTS 68

Query: 61  YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIG 120
            P+SF+ S+++RYG TG+YKA MFG P V+VT+ E C+RVL DD+AF  GWP STMKLIG
Sbjct: 69  DPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIG 128

Query: 121 ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELR 180
             SF+GIS +EHKRLRR+T+APVNG +AL+ Y+ +IE+ V + L+KW  MGEIEFL+ LR
Sbjct: 129 RKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLR 188

Query: 181 KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF 240
           KLTFK+IMYIFLSSESE +M++LEREYT LN GVRAM IN+PGFAY++ALKARK L A F
Sbjct: 189 KLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 248

Query: 241 GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 300
            SIV  RR   +RK+N  + ++DMLD L++ +DE+GR L DEEIID+L+MYLNAGHESSG
Sbjct: 249 QSIVTNRR--NQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSG 308

Query: 301 HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV 360
           H  MWATI +Q+HP  LQKAK EQE ++KKR  GQK LTLKE R+M YLS+VIDETLRV+
Sbjct: 309 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 368

Query: 361 TFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAG 420
           TFSLT FREAK DV++ GY IPKGWKVL WFR+VH D E YP+P++F+PSRW+ +TPKAG
Sbjct: 369 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 428

Query: 421 SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRI 480
           +FLPFG GS LCPGNDLAKLEIS+FLH+FLL Y++ER NP   V +LPH+RP+DNCL RI
Sbjct: 429 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486

Query: 481 KR 483
            R
Sbjct: 489 TR 486

BLAST of Chy11G205290 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.4e-161
Identity = 273/484 (56.40%), Postives = 366/484 (75.62%), Query Frame = 0

Query: 4   AVLLAVIPFLVWLLKNANHLLYEIAQLGS-KRFALPPGNLGWPFIGNMWSFLRAFKSPYP 63
           AV+  V+  LV  +    H    +A LG+ +R  LPPG +GWP +G+MW+FLRAFKS  P
Sbjct: 23  AVVAGVV--LVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNP 82

Query: 64  DSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIGEN 123
           D+F+ SF+ R+G TG+Y+ FMF SP+++  + EACK+VL DDE F +GWP +T+ LIG  
Sbjct: 83  DAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPK 142

Query: 124 SFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWG--YMGEIEFLTELR 183
           SF+ +S  +H+R+R++TAAP+NG+DAL TYL++I++ VV+SL +W     G++EFLTELR
Sbjct: 143 SFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELR 202

Query: 184 KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF 243
           ++TFKII+ IF+S   +  MEALER YT LN G+RAMAIN+PGFAYY+AL+AR+ L +  
Sbjct: 203 RMTFKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVL 262

Query: 244 GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 303
             ++ GRR     K   ++   DM+D L+EAEDE GRRL+D+EI+DVLIMYLNAGHESSG
Sbjct: 263 QGVLDGRR-AAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSG 322

Query: 304 HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV 363
           H  MWAT+FLQ++P    +AKAEQEE+++  P  Q GLTL++ ++M +LS+V+DETLR V
Sbjct: 323 HITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCV 382

Query: 364 TFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAG 423
             S   FR+A  D+ ++GY IPKGWKV +W+RSVH D + YP+P+ FNPSRW+   PKAG
Sbjct: 383 NISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAG 442

Query: 424 SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRI 483
           +FLPFG G+RLCPGNDLAKLEISVFLH+FLL YKL+R NP+  VRYLPH RP DNCL  I
Sbjct: 443 TFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNCLATI 502

Query: 484 KRLA 485
            +++
Sbjct: 503 TKVS 503

BLAST of Chy11G205290 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 3.9e-159
Identity = 273/485 (56.29%), Postives = 354/485 (72.99%), Query Frame = 0

Query: 4   AVLLAVIPFLVWLLKNANHLLYEIAQLGS-KRFALPPGNLGWPFIGNMWSFLRAFKSPYP 63
           AV+ A+    +     A H  Y  A LG+ +R  LPPG++GWP +G MW+FLRAFKS  P
Sbjct: 11  AVVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRP 70

Query: 64  DSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIGEN 123
           DSF+DSF  R+G  G+Y+AFMF SP+++ T+ EACK+VL DD+AF +GWP +T+ LIG  
Sbjct: 71  DSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPK 130

Query: 124 SFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKW---GYMGEIEFLTEL 183
           SF+ +   EH+RLR++TAAP+NG+DAL +YL +I+  VV++L  W   G  G  EFLTEL
Sbjct: 131 SFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTEL 190

Query: 184 RKLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEAT 243
           R++TF+II+ IF+    E     LER YT LN G+RAMAI++PGFAY+KA++AR+ L A 
Sbjct: 191 RRMTFRIIVQIFMGGADERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAA 250

Query: 244 FGSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESS 303
              ++  RR    +         DM+D L+  EDE GRRL D+EIIDVL+MYLNAGHESS
Sbjct: 251 LQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESS 310

Query: 304 GHTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRV 363
           GH  MWAT+FLQ++P  L KAKAEQE +++  P GQKGLTL++ R+M YLS+V+DETLR 
Sbjct: 311 GHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVVDETLRF 370

Query: 364 VTFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKA 423
           V  S   FR+A  DV ++GY IPKGWKV +W+RSVH D + YP+P++F+PSRW+   P+A
Sbjct: 371 VNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRA 430

Query: 424 GSFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGR 483
           G+FLPFG G+RLCPGNDLAKLEISVFLH+FLL YKL R NP   VRYLPH RP DNCL +
Sbjct: 431 GTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPHPRPVDNCLAK 490

Query: 484 IKRLA 485
           I RL+
Sbjct: 491 ITRLS 495

BLAST of Chy11G205290 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 5.6e-158
Identity = 267/464 (57.54%), Postives = 348/464 (75.00%), Query Frame = 0

Query: 22  HLLYEIAQLGSKRFA-LPPGNLGWPFIGNMWSFLRAFKSPYPDSFMDSFLSRYGNTGMYK 81
           H  Y  A LG+ R A LPPG +GWP +G MW+FLRAFKS  PD+F+ SF+ R+G TG+Y+
Sbjct: 56  HGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPDAFIASFVRRFGRTGVYR 115

Query: 82  AFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIGENSFIGISNQEHKRLRRITA 141
           +FMF SP+V+VT+AE CK+VL DD+AF +GWP +T+ L+G  SF+ +   EH+R+R++TA
Sbjct: 116 SFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRIRKLTA 175

Query: 142 APVNGYDALATYLTYIEKIVVSSLDKWG-YMGEIEFLTELRKLTFKIIMYIFLSSESETI 201
           AP+NG+DAL  YL +I++ V SSL  W  + G +EFLTELR++TFKII+ IFL    +  
Sbjct: 176 APINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGGADQAT 235

Query: 202 MEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATFGSIVRGRRMERERKENSKT 261
             ALER YT LN G+RAMAIN+PGFAY  AL+AR+ L A    ++  RR  R +  +   
Sbjct: 236 TRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQGVLDERRAARAKGVSGGG 295

Query: 262 MKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPHFLQK 321
           +  DM+D L+EA+DE GR L D+EIIDVL+MYLNAGHESSGH  MWAT+FLQ++P    +
Sbjct: 296 V--DMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPDMFAR 355

Query: 322 AKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVVTFSLTVFREAKHDVKISGY 381
           AKAEQE +++  P  Q+GLTL++ R+M YLS+VIDETLR+V  S   FR+A  DV ++GY
Sbjct: 356 AKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQATRDVFVNGY 415

Query: 382 TIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAGSFLPFGAGSRLCPGNDLAK 441
            IPKGWKV +W+RSVH D + YP+P +F+PSRW+  +P+AG+FL FG G+RLCPGNDLAK
Sbjct: 416 LIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGARLCPGNDLAK 475

Query: 442 LEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRIKRL 484
           LEISVFLH+FLL YKL R NP   VRYLPH RP DNCL +I R+
Sbjct: 476 LEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAKITRV 517

BLAST of Chy11G205290 vs. ExPASy TrEMBL
Match: A0A0A0KD08 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G079220 PE=3 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.1e-246
Identity = 441/483 (91.30%), Postives = 449/483 (92.96%), Query Frame = 0

Query: 1   MYTAVLLAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP 60
           MYTAVLLAVIPFL+WLLKNANHLLYE AQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP
Sbjct: 1   MYTAVLLAVIPFLLWLLKNANHLLYETAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP 60

Query: 61  YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIG 120
           YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWP STMKLIG
Sbjct: 61  YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPPSTMKLIG 120

Query: 121 ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELR 180
           ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWG MGEIEFLTELR
Sbjct: 121 ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGNMGEIEFLTELR 180

Query: 181 KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF 240
           KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF
Sbjct: 181 KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF 240

Query: 241 GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 300
           GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG
Sbjct: 241 GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 300

Query: 301 HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV 360
           HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV
Sbjct: 301 HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV 360

Query: 361 TFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAG 420
           TFSLTVFREAKHDVKIS Y +      L+ F                    ++DF PKAG
Sbjct: 361 TFSLTVFREAKHDVKISAYKLSNS---LLSF-------------------LFNDFIPKAG 420

Query: 421 SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRI 480
           SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPES +RYLPHS+P+DNCLGRI
Sbjct: 421 SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESPIRYLPHSKPEDNCLGRI 461

Query: 481 KRL 484
           KRL
Sbjct: 481 KRL 461

BLAST of Chy11G205290 vs. ExPASy TrEMBL
Match: A0A6J1KIC3 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111494707 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.9e-227
Identity = 396/486 (81.48%), Postives = 431/486 (88.68%), Query Frame = 0

Query: 1   MYTAVL---LAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAF 60
           MYTAVL   L  +   VW+LKNAN  ++E  +LGSKRF LPPG+LGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSPYPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMK 120
           KS  PDSFM+SF++RYG T MYK FMFGSPSVIVTSAEACKRVLNDD+AFGSGWP ST+K
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPHSTLK 120

Query: 121 LIGENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLT 180
           LIGE SFIGIS +EHKRLRRITAAPVNGY+ALATYLTYIE + VSSL KW  MG+IEFLT
Sbjct: 121 LIGEKSFIGISAKEHKRLRRITAAPVNGYEALATYLTYIENVTVSSLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLE 240
           ELRKLTFKIIMYIFLSSESE +MEALEREYT LN GVRAMAINIPGFAY+KALKARKNL 
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 ATFGSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHE 300
            TF SIV  RR  +ER  N  T +RDM+DALL+ EDE+GRRLSDEEIIDVLIMYLNAGHE
Sbjct: 241 TTFQSIVSQRR--KERDNNVATTRRDMMDALLDVEDENGRRLSDEEIIDVLIMYLNAGHE 300

Query: 301 SSGHTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETL 360
           SSGHTMMWATIFLQQHP+FL+KAKAEQEE++KKR  GQKGLTLKEVR+M YLSKVIDETL
Sbjct: 301 SSGHTMMWATIFLQQHPNFLEKAKAEQEEILKKRSPGQKGLTLKEVREMDYLSKVIDETL 360

Query: 361 RVVTFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTP 420
           R+VTFSL VFREAKHDV I GYTIPKGWKVLVWFRSVH+D ETYPNP++F+PSRWDDFTP
Sbjct: 361 RIVTFSLMVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTP 420

Query: 421 KAGSFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCL 480
           KAGSFLPFG GSRLCPGNDLAKLEISVFLHYFLLNY+LER NP S VRYLPHSRP+D CL
Sbjct: 421 KAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYQLERDNPNSPVRYLPHSRPKDQCL 480

Query: 481 GRIKRL 484
            RIKR+
Sbjct: 481 ARIKRV 484

BLAST of Chy11G205290 vs. ExPASy TrEMBL
Match: A0A6J1HJH6 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464585 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.9e-226
Identity = 394/486 (81.07%), Postives = 432/486 (88.89%), Query Frame = 0

Query: 1   MYTAVL---LAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAF 60
           MYTAVL   L  +   VW+LKNAN  ++E  +LGSKRF LPPG+LGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSPYPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMK 120
           KS  PDSFM+SF++RYG T MYK FMFGSPSVIVTS EACKRVLNDD+AFGSGWP ST+K
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSTEACKRVLNDDDAFGSGWPRSTLK 120

Query: 121 LIGENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLT 180
           LIGE SFIGIS++EHKRLRRITAAPVNGY+ALATYLTYIE + V+SL KW  MG+IEFLT
Sbjct: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLE 240
           ELRKLTFKIIMYIFLSSESE +MEALEREYT LN GVRAMAINIPGFAY+KALKARKNL 
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 ATFGSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHE 300
            TF SIV  RR  ++R  +  T +RDM+DALL+ EDE+GRRLSDEEIIDVLIMYLNAGHE
Sbjct: 241 MTFQSIVSQRR--KKRDNDVATTRRDMMDALLDVEDENGRRLSDEEIIDVLIMYLNAGHE 300

Query: 301 SSGHTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETL 360
           SSGHTMMWATIFLQQHP+FL KAKAEQEE++KKRP GQKGLTLKEVR+M YLSKVIDETL
Sbjct: 301 SSGHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETL 360

Query: 361 RVVTFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTP 420
           RVVTFSLTVFREAKHDV I GYTIPKGWKVLVWFRSVH+D ETYPNP++F+PSRWDDFTP
Sbjct: 361 RVVTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTP 420

Query: 421 KAGSFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCL 480
           KAGSFLPFG GSRLCPGNDLAKLEISVFLHYFLLNY+LER NP S VRYLPH+RP+D CL
Sbjct: 421 KAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCL 480

Query: 481 GRIKRL 484
            RIKR+
Sbjct: 481 ARIKRV 484

BLAST of Chy11G205290 vs. ExPASy TrEMBL
Match: A0A6J1DNE5 (ent-kaurenoic acid oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111022826 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 9.1e-220
Identity = 376/479 (78.50%), Postives = 418/479 (87.27%), Query Frame = 0

Query: 5   VLLAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSPYPDS 64
           V  A +   VW+ KNAN  +YE  +LG KRF LPPG+LGWPF+GNMW FLRAFKS  PDS
Sbjct: 9   VAAAAVAAAVWVAKNANRWVYENRRLGEKRFWLPPGDLGWPFVGNMWDFLRAFKSSDPDS 68

Query: 65  FMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIGENSF 124
           FM SF+SRYG  G+YK+ MFGSPSV+V+SAEACKRVL DD+AFGSGWP+STMKLIGE SF
Sbjct: 69  FMASFVSRYGRRGIYKSLMFGSPSVLVSSAEACKRVLTDDDAFGSGWPVSTMKLIGEKSF 128

Query: 125 IGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELRKLTF 184
           IGIS QEHKRLRRITAAPVNGY+ALATYL YIE+ VV SL KW  MG+IEFLT LRKLTF
Sbjct: 129 IGISYQEHKRLRRITAAPVNGYEALATYLKYIEQTVVVSLQKWATMGQIEFLTHLRKLTF 188

Query: 185 KIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATFGSIV 244
            IIM+IFLSSESET+M+A+EREYT LN GVRAMAINIPGFAY+KALKAR+NL ATF SIV
Sbjct: 189 NIIMHIFLSSESETVMDAMEREYTKLNYGVRAMAINIPGFAYHKALKARRNLVATFQSIV 248

Query: 245 RGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSGHTMM 304
             RR ERE   N  T +RDM+DALL+ EDE+GR+LSDEEIIDVLIMYLNAGHESSGHTMM
Sbjct: 249 TNRRKERE--NNLATKRRDMMDALLDVEDENGRKLSDEEIIDVLIMYLNAGHESSGHTMM 308

Query: 305 WATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVVTFSL 364
           WATIFLQQHP F +KAK EQEE++++RP  QKGLTLKEVR+M YLSKVIDETLRVVTFSL
Sbjct: 309 WATIFLQQHPEFFEKAKIEQEEILRRRPASQKGLTLKEVREMYYLSKVIDETLRVVTFSL 368

Query: 365 TVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAGSFLP 424
            VFREAKHDV ISGY IPKGWK+LVWFR VH D +TYPNP+EFNPSRWDDFTPKAGSFLP
Sbjct: 369 MVFREAKHDVNISGYLIPKGWKILVWFRGVHLDPQTYPNPKEFNPSRWDDFTPKAGSFLP 428

Query: 425 FGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRIKRL 484
           FGAGSRLCPGNDLAKLE+S+FLHYFLLNY+LER NP S VRYLPHSRP+DNCL R+KR+
Sbjct: 429 FGAGSRLCPGNDLAKLEVSIFLHYFLLNYQLERANPNSPVRYLPHSRPKDNCLARVKRV 485

BLAST of Chy11G205290 vs. ExPASy TrEMBL
Match: A0A5D3DLR2 (Ent-kaurenoic acid oxidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00760 PE=3 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.6e-214
Identity = 369/414 (89.13%), Postives = 396/414 (95.65%), Query Frame = 0

Query: 72  RYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIGENSFIGISNQE 131
           +YGNTG+YKAFMFGS SVIVTS E CKRVLNDDEAFG GWPLST+KLIGENSF+GIS QE
Sbjct: 8   KYGNTGVYKAFMFGSASVIVTSPETCKRVLNDDEAFGPGWPLSTLKLIGENSFVGISYQE 67

Query: 132 HKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELRKLTFKIIMYIF 191
           HKRLRRITAAPVNGY+AL+TYLTYIEKIVVSSL+KW YMGEIEFLTELR+LTFK+IMYIF
Sbjct: 68  HKRLRRITAAPVNGYEALSTYLTYIEKIVVSSLEKWAYMGEIEFLTELRQLTFKVIMYIF 127

Query: 192 LSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATFGSIVRGRRMER 251
           LSSESE I EALEREYTMLNLGVRAMAINIPGFAY+KALKARKNLEATFGSIVR RRMER
Sbjct: 128 LSSESEAITEALEREYTMLNLGVRAMAINIPGFAYHKALKARKNLEATFGSIVRERRMER 187

Query: 252 ERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQ 311
           ERKENS+  +RDM+DALLEAEDEDGRRLSDEEIIDVL+MYLNAGHESSGHTMMWA IFL 
Sbjct: 188 ERKENSEGKRRDMMDALLEAEDEDGRRLSDEEIIDVLLMYLNAGHESSGHTMMWAAIFLH 247

Query: 312 QHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVR-QMPYLSKVIDETLRVVTFSLTVFREA 371
           QHP FLQKAKAEQEE+++KRP+GQKGLTLKEVR QMPYL+KVIDETLRVVTFSLTVFREA
Sbjct: 248 QHPQFLQKAKAEQEEIVRKRPLGQKGLTLKEVRSQMPYLTKVIDETLRVVTFSLTVFREA 307

Query: 372 KHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAGSFLPFGAGSR 431
           KHDVKISGYTIPKGWKVLVWFRSVHFD ETYPNP+EFNPSRWDDFTPKAGSFLPFGAGSR
Sbjct: 308 KHDVKISGYTIPKGWKVLVWFRSVHFDPETYPNPKEFNPSRWDDFTPKAGSFLPFGAGSR 367

Query: 432 LCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRIKRLA 485
           LCPGNDLAKLE+++FLH+FLLNYKLERVNPES VRYLPH+RP+DNCL RIKR+A
Sbjct: 368 LCPGNDLAKLEVAIFLHHFLLNYKLERVNPESPVRYLPHTRPKDNCLARIKRVA 421

BLAST of Chy11G205290 vs. NCBI nr
Match: XP_011656712.1 (ent-kaurenoic acid oxidase 1 [Cucumis sativus] >KGN46284.2 hypothetical protein Csa_004971 [Cucumis sativus])

HSP 1 Score: 948 bits (2451), Expect = 0.0
Identity = 473/484 (97.73%), Postives = 478/484 (98.76%), Query Frame = 0

Query: 1   MYTAVLLAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP 60
           MYTAVLLAVIPFL+WLLKNANHLLYE AQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP
Sbjct: 1   MYTAVLLAVIPFLLWLLKNANHLLYETAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP 60

Query: 61  YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIG 120
           YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWP STMKLIG
Sbjct: 61  YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPPSTMKLIG 120

Query: 121 ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELR 180
           ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWG MGEIEFLTELR
Sbjct: 121 ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGNMGEIEFLTELR 180

Query: 181 KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF 240
           KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF
Sbjct: 181 KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF 240

Query: 241 GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 300
           GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG
Sbjct: 241 GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 300

Query: 301 HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV 360
           HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV
Sbjct: 301 HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV 360

Query: 361 TFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAG 420
           TFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYP+PREFNPSRWDDF PKAG
Sbjct: 361 TFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPDPREFNPSRWDDFIPKAG 420

Query: 421 SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRI 480
           SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPES +RYLPHS+P+DNCLGRI
Sbjct: 421 SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESPIRYLPHSKPEDNCLGRI 480

Query: 481 KRLA 484
           KRL 
Sbjct: 481 KRLV 484

BLAST of Chy11G205290 vs. NCBI nr
Match: XP_038907052.1 (ent-kaurenoic acid oxidase 2-like [Benincasa hispida])

HSP 1 Score: 859 bits (2219), Expect = 1.43e-312
Identity = 427/483 (88.41%), Postives = 451/483 (93.37%), Query Frame = 0

Query: 1   MYTAVLLAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP 60
           M+ AVL+AV+   VW+LKNANH ++E A+LGSKRFALPPG+LGWPFIGNMWSFLRAFKSP
Sbjct: 1   MFMAVLVAVLVVAVWVLKNANHWVHETARLGSKRFALPPGDLGWPFIGNMWSFLRAFKSP 60

Query: 61  YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIG 120
           YPDSF+DSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWP STMKLIG
Sbjct: 61  YPDSFIDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPTSTMKLIG 120

Query: 121 ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELR 180
           ENSFIGIS QEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSL+KW  MG+IEFLTELR
Sbjct: 121 ENSFIGISYQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLEKWASMGQIEFLTELR 180

Query: 181 KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF 240
           KLTFKIIMYIFLSSESE IMEALEREYT LN GVRAMAINIPGFAY KALKARKNL ATF
Sbjct: 181 KLTFKIIMYIFLSSESEAIMEALEREYTQLNHGVRAMAINIPGFAYNKALKARKNLVATF 240

Query: 241 GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 300
           GSIV GRR ERERK N    +RDM+DALL+AEDEDGRRLSDEEIIDVLIMYLNAGHESSG
Sbjct: 241 GSIVSGRRKERERKNNLIAKRRDMMDALLDAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 300

Query: 301 HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV 360
           HTMMWATIFLQQHP FLQKAK EQEE++KKRP+GQK LTLKEVR+MPYLSKVIDETLRVV
Sbjct: 301 HTMMWATIFLQQHPQFLQKAKVEQEEIVKKRPLGQKNLTLKEVREMPYLSKVIDETLRVV 360

Query: 361 TFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAG 420
           TFSLTVFREAK DVKI GYTIPKGWKVLVWFRSVH+D ETYPNP+EFNPSRWD+F PKAG
Sbjct: 361 TFSLTVFREAKQDVKICGYTIPKGWKVLVWFRSVHYDPETYPNPKEFNPSRWDNFIPKAG 420

Query: 421 SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRI 480
           SFLPFGAGSRLCPGNDLAKLEIS+FLHYFLLNY+LERVNP S VRYLPHSRP+DNCL RI
Sbjct: 421 SFLPFGAGSRLCPGNDLAKLEISIFLHYFLLNYQLERVNPRSPVRYLPHSRPKDNCLARI 480

Query: 481 KRL 483
           KRL
Sbjct: 481 KRL 483

BLAST of Chy11G205290 vs. NCBI nr
Match: XP_023513658.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 793 bits (2049), Expect = 1.35e-286
Identity = 396/487 (81.31%), Postives = 433/487 (88.91%), Query Frame = 0

Query: 1   MYTAVL---LAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAF 60
           MYTAVL   L  +   +W+LKNAN  ++E  +LGSKRF LPPG+LGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATIWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSPYPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMK 120
           KS  PDSFM+SF++RYG T MYK FMFGSPSVIVTSAEACKRVLNDD+AFGSGWP ST+K
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120

Query: 121 LIGENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLT 180
           LIGE SFIGIS++EHKRLRRITAAPVNGY+ALATYLTYIE + V+SL KW  MG+IEFLT
Sbjct: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLE 240
           ELRKLTFKIIMYIFLSSESE +MEALEREYT LN GVRAMAINIPGFAY+KALKARKNL 
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 ATFGSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHE 300
            TF SIV  RR ER+   N  T +RDM+DALL+ EDE+GRRLSDEEIIDVLIMYLNAGHE
Sbjct: 241 TTFQSIVSQRRKERDN--NVATTRRDMMDALLDVEDENGRRLSDEEIIDVLIMYLNAGHE 300

Query: 301 SSGHTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETL 360
           SSGHTMMWATIFLQQHP+FL KAKAEQEE++KKRP GQKGLTLKEVR+M YLSKVIDETL
Sbjct: 301 SSGHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETL 360

Query: 361 RVVTFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTP 420
           RVVTFSLTVFREAKHDV I GYTIPKGWKVLVWFRSVH+D ETYPNP++F+PSRWDDFTP
Sbjct: 361 RVVTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTP 420

Query: 421 KAGSFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCL 480
           KAGSFLPFG GSRLCPGNDLAKLEISVFLHYFLLNY+LER NP S VRYLPH+RP+D CL
Sbjct: 421 KAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYQLERDNPNSPVRYLPHTRPKDQCL 480

Query: 481 GRIKRLA 484
            RIKR+ 
Sbjct: 481 ARIKRVG 485

BLAST of Chy11G205290 vs. NCBI nr
Match: XP_023000440.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita maxima])

HSP 1 Score: 791 bits (2043), Expect = 1.10e-285
Identity = 396/487 (81.31%), Postives = 431/487 (88.50%), Query Frame = 0

Query: 1   MYTAVL---LAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAF 60
           MYTAVL   L  +   VW+LKNAN  ++E  +LGSKRF LPPG+LGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSPYPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMK 120
           KS  PDSFM+SF++RYG T MYK FMFGSPSVIVTSAEACKRVLNDD+AFGSGWP ST+K
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPHSTLK 120

Query: 121 LIGENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLT 180
           LIGE SFIGIS +EHKRLRRITAAPVNGY+ALATYLTYIE + VSSL KW  MG+IEFLT
Sbjct: 121 LIGEKSFIGISAKEHKRLRRITAAPVNGYEALATYLTYIENVTVSSLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLE 240
           ELRKLTFKIIMYIFLSSESE +MEALEREYT LN GVRAMAINIPGFAY+KALKARKNL 
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 ATFGSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHE 300
            TF SIV  RR ER+   N  T +RDM+DALL+ EDE+GRRLSDEEIIDVLIMYLNAGHE
Sbjct: 241 TTFQSIVSQRRKERDN--NVATTRRDMMDALLDVEDENGRRLSDEEIIDVLIMYLNAGHE 300

Query: 301 SSGHTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETL 360
           SSGHTMMWATIFLQQHP+FL+KAKAEQEE++KKR  GQKGLTLKEVR+M YLSKVIDETL
Sbjct: 301 SSGHTMMWATIFLQQHPNFLEKAKAEQEEILKKRSPGQKGLTLKEVREMDYLSKVIDETL 360

Query: 361 RVVTFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTP 420
           R+VTFSL VFREAKHDV I GYTIPKGWKVLVWFRSVH+D ETYPNP++F+PSRWDDFTP
Sbjct: 361 RIVTFSLMVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTP 420

Query: 421 KAGSFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCL 480
           KAGSFLPFG GSRLCPGNDLAKLEISVFLHYFLLNY+LER NP S VRYLPHSRP+D CL
Sbjct: 421 KAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYQLERDNPNSPVRYLPHSRPKDQCL 480

Query: 481 GRIKRLA 484
            RIKR+ 
Sbjct: 481 ARIKRVG 485

BLAST of Chy11G205290 vs. NCBI nr
Match: KAG6593731.1 (Ent-kaurenoic acid oxidase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026070.1 Ent-kaurenoic acid oxidase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 790 bits (2039), Expect = 4.48e-285
Identity = 395/487 (81.11%), Postives = 432/487 (88.71%), Query Frame = 0

Query: 1   MYTAVL---LAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAF 60
           MYTAVL   L  +   VW+LKNAN  ++E  +LGSKRF LPPG+LGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSPYPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMK 120
           KS  PDSFM+SF++RYG T MYK FMFGSPSVIVTSAEACKRVLNDD+AFGSGWP ST+K
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120

Query: 121 LIGENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLT 180
           LIGE SFIGIS++EHKRLRRITAAPVNGY+ALATYLTYIE + V+SL KW  MG+IEFLT
Sbjct: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLE 240
           ELRKLTFKIIMYIFLSSESE +MEALEREYT LN GVRAMAINIPGFAY+KALKARKNL 
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 ATFGSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHE 300
            TF SIV  RR ER+   +  T +RDM+DALL+ EDE+GR LSDEEIIDVLIMYLNAGHE
Sbjct: 241 MTFQSIVSQRRKERDN--DVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHE 300

Query: 301 SSGHTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETL 360
           SSGHTMMWATIFLQQHP+FL KAKAEQEE++KKRP GQKGLTLKEVR+M YLSKVIDETL
Sbjct: 301 SSGHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETL 360

Query: 361 RVVTFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTP 420
           RVVTFSLTVFREAKHDV I GYTIPKGWKVLVWFRSVH+D ETYPNP++F+PSRWDDFTP
Sbjct: 361 RVVTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTP 420

Query: 421 KAGSFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCL 480
           KAGSFLPFG GSRLCPGNDLAKLEISVFLHYFLLNY+LER NP S VRYLPH+RP+D CL
Sbjct: 421 KAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCL 480

Query: 481 GRIKRLA 484
            RIKR+ 
Sbjct: 481 ARIKRVG 485

BLAST of Chy11G205290 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 664.1 bits (1712), Expect = 8.7e-191
Identity = 321/469 (68.44%), Postives = 388/469 (82.73%), Query Frame = 0

Query: 13  LVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSPYPDSFMDSFLSR 72
           L WL++  N  LYE + LG  R  LPPG+LGWPFIGNM SFLRAFK+  PDSF  + + R
Sbjct: 22  LNWLVRKVNVWLYE-SSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKR 81

Query: 73  YGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIGENSFIGISNQEH 132
           YG  G+YKA MFG+PS+IVT+++ C+RVL DD+AF  GWP STM+LIG  SF+GIS +EH
Sbjct: 82  YGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEH 141

Query: 133 KRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELRKLTFKIIMYIFL 192
           KRLRR+TAAPVNG++AL+TY+ YIE+ V++ LDKW  MGE EFLT LRKLTF+IIMYIFL
Sbjct: 142 KRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIFL 201

Query: 193 SSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATFGSIVRGRRMERE 252
           SSESE +M+ALEREYT LN GVRAMA+NIPGFAY++ALKARK L A F SIV  RR   +
Sbjct: 202 SSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERR--NQ 261

Query: 253 RKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQ 312
           RK+N  + K+DMLD LL  +DEDG+ L DEEIIDVL+MYLNAGHESSGHT+MWAT+FLQ+
Sbjct: 262 RKQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQE 321

Query: 313 HPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVVTFSLTVFREAKH 372
           HP  LQ+AKAEQE ++K RP GQKGL+LKE R+M +LS+V+DETLRV+TFSLT FREAK 
Sbjct: 322 HPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKT 381

Query: 373 DVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDD-FTPKAGSFLPFGAGSRL 432
           DV+++GY IPKGWKVL WFR VH D E +P+PR+F+P+RWD+ F PKAG+FLPFGAGS L
Sbjct: 382 DVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHL 441

Query: 433 CPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRI 481
           CPGNDLAKLEIS+FLH+FLL Y+++R NPE  V YLPH+RP DNCL RI
Sbjct: 442 CPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of Chy11G205290 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-185
Identity = 313/482 (64.94%), Postives = 390/482 (80.91%), Query Frame = 0

Query: 1   MYTAVLLAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP 60
           M+  +++  +  L W+LK  N  +Y +++LG K+  LPPG+LGWP IGNMWSFLRAFK+ 
Sbjct: 9   MWFPLIILGLFVLKWVLKRVNVWIY-VSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTS 68

Query: 61  YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIG 120
            P+SF+ S+++RYG TG+YKA MFG P V+VT+ E C+RVL DD+AF  GWP STMKLIG
Sbjct: 69  DPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIG 128

Query: 121 ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELR 180
             SF+GIS +EHKRLRR+T+APVNG +AL+ Y+ +IE+ V + L+KW  MGEIEFL+ LR
Sbjct: 129 RKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLR 188

Query: 181 KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF 240
           KLTFK+IMYIFLSSESE +M++LEREYT LN GVRAM IN+PGFAY++ALKARK L A F
Sbjct: 189 KLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 248

Query: 241 GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 300
            SIV  RR   +RK+N  + ++DMLD L++ +DE+GR L DEEIID+L+MYLNAGHESSG
Sbjct: 249 QSIVTNRR--NQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSG 308

Query: 301 HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV 360
           H  MWATI +Q+HP  LQKAK EQE ++KKR  GQK LTLKE R+M YLS+VIDETLRV+
Sbjct: 309 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 368

Query: 361 TFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAG 420
           TFSLT FREAK DV++ GY IPKGWKVL WFR+VH D E YP+P++F+PSRW+ +TPKAG
Sbjct: 369 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 428

Query: 421 SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRI 480
           +FLPFG GS LCPGNDLAKLEIS+FLH+FLL Y++ER NP   V +LPH+RP+DNCL RI
Sbjct: 429 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486

Query: 481 KR 483
            R
Sbjct: 489 TR 486

BLAST of Chy11G205290 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-185
Identity = 313/482 (64.94%), Postives = 390/482 (80.91%), Query Frame = 0

Query: 1   MYTAVLLAVIPFLVWLLKNANHLLYEIAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSP 60
           M+  +++  +  L W+LK  N  +Y +++LG K+  LPPG+LGWP IGNMWSFLRAFK+ 
Sbjct: 9   MWFPLIILGLFVLKWVLKRVNVWIY-VSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTS 68

Query: 61  YPDSFMDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPLSTMKLIG 120
            P+SF+ S+++RYG TG+YKA MFG P V+VT+ E C+RVL DD+AF  GWP STMKLIG
Sbjct: 69  DPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIG 128

Query: 121 ENSFIGISNQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELR 180
             SF+GIS +EHKRLRR+T+APVNG +AL+ Y+ +IE+ V + L+KW  MGEIEFL+ LR
Sbjct: 129 RKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLR 188

Query: 181 KLTFKIIMYIFLSSESETIMEALEREYTMLNLGVRAMAINIPGFAYYKALKARKNLEATF 240
           KLTFK+IMYIFLSSESE +M++LEREYT LN GVRAM IN+PGFAY++ALKARK L A F
Sbjct: 189 KLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 248

Query: 241 GSIVRGRRMERERKENSKTMKRDMLDALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSG 300
            SIV  RR   +RK+N  + ++DMLD L++ +DE+GR L DEEIID+L+MYLNAGHESSG
Sbjct: 249 QSIVTNRR--NQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSG 308

Query: 301 HTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVV 360
           H  MWATI +Q+HP  LQKAK EQE ++KKR  GQK LTLKE R+M YLS+VIDETLRV+
Sbjct: 309 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 368

Query: 361 TFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAG 420
           TFSLT FREAK DV++ GY IPKGWKVL WFR+VH D E YP+P++F+PSRW+ +TPKAG
Sbjct: 369 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 428

Query: 421 SFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKLERVNPESTVRYLPHSRPQDNCLGRI 480
           +FLPFG GS LCPGNDLAKLEIS+FLH+FLL Y++ER NP   V +LPH+RP+DNCL RI
Sbjct: 429 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486

Query: 481 KR 483
            R
Sbjct: 489 TR 486

BLAST of Chy11G205290 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 251.5 bits (641), Expect = 1.4e-66
Identity = 152/453 (33.55%), Postives = 241/453 (53.20%), Query Frame = 0

Query: 32  SKRFALPPGNLGWPFIGNMWSFLRAFKSPYPDSFMDSFLSRYGNTGMYKAFMFGSPSVIV 91
           + R  LPPG++GWP++G          S  P+ F  S   RYG   ++K  + G P V++
Sbjct: 29  NSRGKLPPGSMGWPYLGETLQLY----SQNPNVFFTSKQKRYGE--IFKTRILGYPCVML 88

Query: 92  TSAEACKRVL-NDDEAFGSGWPLSTMKLIGENSFIGISNQEHKRLRRITAAPVNGYDALA 151
            S EA + VL      F   +P S  KLIG ++        H  +R++  +     + + 
Sbjct: 89  ASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYP-ETIR 148

Query: 152 TYLTYIEKIVVSSLDKWGYMGEIEFLTELRKLTFKI-IMYIFLSSESETIMEALEREYTM 211
             +  IE I +SSL  W  M  +    E++K  F + I+ IF   ES +  E L+  Y +
Sbjct: 149 KLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHLES-SYKEILKHNYNI 208

Query: 212 LNLGVRAMAINIPGFAYYKALKARKNLEATFGSIVRGRRMERERKENSKTMKRDMLDALL 271
           ++ G  +  +++PG +Y+KAL ARK L+     I+  RR +R        ++ D L  LL
Sbjct: 209 VDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKR-------ALQTDFLGHLL 268

Query: 272 EAEDEDGRRLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPHFLQKAKAEQEEMIK 331
             ++E GR L+ E+I D +I  L A  +++   + W   +L      L+  KAEQ+ + +
Sbjct: 269 NFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIYE 328

Query: 332 KRPVGQKGLTLKEVRQMPYLSKVIDETLRVVTFSLTVFREAKHDVKISGYTIPKGWKVLV 391
           +    +K LT ++ R MP   KVI E+LR+ +     FREA  DV+  GY IPKGWKV+ 
Sbjct: 329 ENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMP 388

Query: 392 WFRSVHFDSETYPNPREFNPSRWDDFTPKAGSFLPFGAGSRLCPGNDLAKLEISVFLHYF 451
            FR++H + + + NP  F+PSR+ +  PK  +F+PFG+G   CPGN+LAKL+I +FLH+ 
Sbjct: 389 LFRNIHHNPKYFSNPEVFDPSRF-EVNPKPNTFMPFGSGVHACPGNELAKLQILIFLHHL 448

Query: 452 LLNYKLERVNPESTVRYLPHSRPQDNCLGRIKR 483
           + N++ E    E  ++Y P   PQ+      +R
Sbjct: 449 VSNFRWEVKGGEKGIQYSPFPIPQNGLPATFRR 465

BLAST of Chy11G205290 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 245.4 bits (625), Expect = 9.7e-65
Identity = 145/449 (32.29%), Postives = 238/449 (53.01%), Query Frame = 0

Query: 27  IAQLGSKRFALPPGNLGWPFIGNMWSFLRAFKSPYPDSFMDSFLSRYGNTGMYKAFMFGS 86
           + +  S +  LPPG +G+P++G  +       S  P+ F  +   RYG+  ++K  + G 
Sbjct: 26  VRRSSSTKLPLPPGTMGYPYVGETFQLY----SQDPNVFFAAKQRRYGS--VFKTHVLGC 85

Query: 87  PSVIVTSAEACKRVL-NDDEAFGSGWPLSTMKLIGENSFIGISNQEHKRLRRITAAPVNG 146
           P V+++S EA K VL      F   +P S  +++G+ +        H +LR++       
Sbjct: 86  PCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMP 145

Query: 147 YDALATYLTYIEKIVVSSLDKWGYMGEIEFLTELRKLTFKIIMYIFLSSESETIMEALER 206
            DA+   + +IE I   SL+ W    ++    E++  TF + +   L  +     E L+R
Sbjct: 146 -DAIRNMVPHIESIAQESLNSWDGT-QLNTYQEMKTYTFNVALISILGKDEVYYREDLKR 205

Query: 207 EYTMLNLGVRAMAINIPGFAYYKALKARKNLEATFGSIVRGRRMERERKENSKTMKRDML 266
            Y +L  G  +M IN+PG  ++KA+KARK L     +I+  RR        + +   D+L
Sbjct: 206 CYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRR-------QNPSSHTDLL 265

Query: 267 DALLEAEDEDGRRLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPHFLQKAKAEQE 326
            + +    ED   L+DE+I D +I  + A  +++   + W   +L  +P  L+    EQ 
Sbjct: 266 GSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQM 325

Query: 327 EMIKKRPVGQKGLTLKEVRQMPYLSKVIDETLRVVTFSLTVFREAKHDVKISGYTIPKGW 386
            + K +  G+  LT ++ ++MP   +VI ETLR  T     FREA  DV+  GY IPKGW
Sbjct: 326 AIRKDKKEGE-SLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGW 385

Query: 387 KVLVWFRSVHFDSETYPNPREFNPSRWDDFTPKAGSFLPFGAGSRLCPGNDLAKLEISVF 446
           KVL  FR++H +++ + +P +F+PSR+ +  PK  +F+PFG+G   CPGN+LAKLEISV 
Sbjct: 386 KVLPLFRNIHHNADIFSDPGKFDPSRF-EVAPKPNTFMPFGSGIHSCPGNELAKLEISVL 445

Query: 447 LHYFLLNYKLERVNPESTVRYLPHSRPQD 475
           +H+    Y+   V P   ++Y P + PQ+
Sbjct: 446 IHHLTTKYRWSIVGPSDGIQYGPFALPQN 453

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O230511.2e-18968.44Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q9C5Y21.6e-18464.94Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
Q5VRM71.4e-16156.40Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Q9AXH93.9e-15956.29Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1[more]
Q432465.6e-15857.54Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KD081.1e-24691.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G079220 PE=3 SV=1[more]
A0A6J1KIC35.9e-22781.48ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111494707 PE... [more]
A0A6J1HJH62.9e-22681.07ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464585 ... [more]
A0A6J1DNE59.1e-22078.50ent-kaurenoic acid oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111022826... [more]
A0A5D3DLR22.6e-21489.13Ent-kaurenoic acid oxidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
XP_011656712.10.097.73ent-kaurenoic acid oxidase 1 [Cucumis sativus] >KGN46284.2 hypothetical protein ... [more]
XP_038907052.11.43e-31288.41ent-kaurenoic acid oxidase 2-like [Benincasa hispida][more]
XP_023513658.11.35e-28681.31ent-kaurenoic acid oxidase 2-like [Cucurbita pepo subsp. pepo][more]
XP_023000440.11.10e-28581.31ent-kaurenoic acid oxidase 2-like [Cucurbita maxima][more]
KAG6593731.14.48e-28581.11Ent-kaurenoic acid oxidase 2, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
Match NameE-valueIdentityDescription
AT1G05160.18.7e-19168.44cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT2G32440.11.1e-18564.94ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.21.1e-18564.94ent-kaurenoic acid hydroxylase 2 [more]
AT3G19270.11.4e-6633.55cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT5G45340.19.7e-6532.29cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 379..393
score: 27.44
coord: 432..450
score: 34.01
coord: 52..75
score: 23.72
coord: 395..413
score: 34.21
coord: 285..311
score: 25.93
coord: 346..362
score: 38.24
IPR001128Cytochrome P450PRINTSPR00385P450coord: 351..362
score: 34.95
coord: 294..311
score: 26.29
coord: 432..443
score: 39.45
coord: 423..432
score: 59.89
IPR001128Cytochrome P450PFAMPF00067p450coord: 38..457
e-value: 8.0E-67
score: 226.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 28..483
e-value: 5.6E-108
score: 363.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 33..475
NoneNo IPR availablePANTHERPTHR24286:SF289ENT-KAURENOIC ACID OXIDASE 2coord: 12..474
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 12..474
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 425..434

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G205290.1Chy11G205290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051777 ent-kaurenoate oxidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen