Chy11G197940 (gene) Cucumber (hystrix) v1

Overview


Name	Chy11G197940
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	RING-type E3 ubiquitin transferase
Location	chrH11: 18323587 .. 18324723 (+)
RNA-Seq Expression	Chy11G197940
Synteny	Chy11G197940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTACCGGAACCGCCTCCTATTGGTGTTACCGCTGCAACCGTTTCGTTCGTGCTTGGGCTCAGGACTCCATCACTTGTCCTTACTGTGACGGTGGCTTTGTTGAAGCTATTGAGACCGCTTCTTCTCTTCCGGCCTCCAATCTCCACCGTCGCTTATCTCCCTCTGCGATCCATACTGTGGATCAAGATTCCTTTCAGTCTCCTCGTTTGAGTACCCGCCGTGGTCGTCGGAGACTTGGGGATCGGTCCACTTTTAACCCTGTTGTTGTTCTTCGTGGTTCTGCTGATGCCGGGGATGTGGTTGGCGGTGGGGAAAGGAATAGCTTTGATATTTACTATGACGATGGTGCTGGCTCTGGGCTTCGCCCGGTTCCGGCGACCATGTCTGAGTTTTTGATGGGGACTGGGTTCGATCGGTTGCTCGAGCAGTTGGCTCAACTGGAGATCAACGGGTTTGGTAGATCTGAAAATCCGCCGGCGTCAAAAGCTGCTGTGGAATCAATGCCGACGATCGAGATTTTAGAAAGTCATGTGGATTCTGATTCGCACTGCGCCGTGTGTAAAGAAGCCTTTGAAATCGGAACTGATGCCCGAGAAATGCCGTGTAAGCATATATATCACTCCGAATGCATAATCCCTTGGCTTTCCATGAGAAATTCCTGTCCGGTCTGCCGGCACGAACTGCCCTCGGAGCGTGTGTCTCCGGCTGGTGGGGTTTCAGATCGAGTTGTTGACGAAGAAACCGTAGGGTTAACGATTTGGAGGCTTCCCGGTGGTGGGTTTGCCGTCGGACGATTCTCCGGCGGAAGATTGGCCGGCGAGAGAGACCCACCGGCTGTGTACACCGAAATGGACGGTGGGTTCAATGGGAACGGAGTTCCAAGGAGGGTTTCGTGGGCTTCGAGAAGAAGCAGGGGAAGAGAGAGCCGAGGAATTGGACGAACATTTCGGAACATATTTTCCTTCTTTGGGAGATTTAGGTCGTCGAGTTCATCTTCTTCCTCGCCTTCAAATGAATCTGAGTCTGTAAGTAGAAGCCATAGCCATTCGAGTTCTTCAGTTTTCAGCAGATACTTACGAAGGCAAAACAGAGCTTGGGTGTTGGAGAACCAGAATGAAAATGGCAGATGGTGA

mRNA sequence

ATGTCTACCGGAACCGCCTCCTATTGGTGTTACCGCTGCAACCGTTTCGTTCGTGCTTGGGCTCAGGACTCCATCACTTGTCCTTACTGTGACGGTGGCTTTGTTGAAGCTATTGAGACCGCTTCTTCTCTTCCGGCCTCCAATCTCCACCGTCGCTTATCTCCCTCTGCGATCCATACTGTGGATCAAGATTCCTTTCAGTCTCCTCGTTTGAGTACCCGCCGTGGTCGTCGGAGACTTGGGGATCGGTCCACTTTTAACCCTGTTGTTGTTCTTCGTGGTTCTGCTGATGCCGGGGATGTGGTTGGCGGTGGGGAAAGGAATAGCTTTGATATTTACTATGACGATGGTGCTGGCTCTGGGCTTCGCCCGGTTCCGGCGACCATGTCTGAGTTTTTGATGGGGACTGGGTTCGATCGGTTGCTCGAGCAGTTGGCTCAACTGGAGATCAACGGGTTTGGTAGATCTGAAAATCCGCCGGCGTCAAAAGCTGCTGTGGAATCAATGCCGACGATCGAGATTTTAGAAAGTCATGTGGATTCTGATTCGCACTGCGCCGTGTGTAAAGAAGCCTTTGAAATCGGAACTGATGCCCGAGAAATGCCGTGTAAGCATATATATCACTCCGAATGCATAATCCCTTGGCTTTCCATGAGAAATTCCTGTCCGGTCTGCCGGCACGAACTGCCCTCGGAGCGTGTGTCTCCGGCTGGTGGGGTTTCAGATCGAGTTGTTGACGAAGAAACCGTAGGGTTAACGATTTGGAGGCTTCCCGGTGGTGGGTTTGCCGTCGGACGATTCTCCGGCGGAAGATTGGCCGGCGAGAGAGACCCACCGGCTGTGTACACCGAAATGGACGGTGGGTTCAATGGGAACGGAGTTCCAAGGAGGGTTTCGTGGGCTTCGAGAAGAAGCAGGGGAAGAGAGAGCCGAGGAATTGGACGAACATTTCGGAACATATTTTCCTTCTTTGGGAGATTTAGGTCGTCGAGTTCATCTTCTTCCTCGCCTTCAAATGAATCTGAGTCTGTAAGTAGAAGCCATAGCCATTCGAGTTCTTCAGTTTTCAGCAGATACTTACGAAGGCAAAACAGAGCTTGGGTGTTGGAGAACCAGAATGAAAATGGCAGATGGTGA

Coding sequence (CDS)

ATGTCTACCGGAACCGCCTCCTATTGGTGTTACCGCTGCAACCGTTTCGTTCGTGCTTGGGCTCAGGACTCCATCACTTGTCCTTACTGTGACGGTGGCTTTGTTGAAGCTATTGAGACCGCTTCTTCTCTTCCGGCCTCCAATCTCCACCGTCGCTTATCTCCCTCTGCGATCCATACTGTGGATCAAGATTCCTTTCAGTCTCCTCGTTTGAGTACCCGCCGTGGTCGTCGGAGACTTGGGGATCGGTCCACTTTTAACCCTGTTGTTGTTCTTCGTGGTTCTGCTGATGCCGGGGATGTGGTTGGCGGTGGGGAAAGGAATAGCTTTGATATTTACTATGACGATGGTGCTGGCTCTGGGCTTCGCCCGGTTCCGGCGACCATGTCTGAGTTTTTGATGGGGACTGGGTTCGATCGGTTGCTCGAGCAGTTGGCTCAACTGGAGATCAACGGGTTTGGTAGATCTGAAAATCCGCCGGCGTCAAAAGCTGCTGTGGAATCAATGCCGACGATCGAGATTTTAGAAAGTCATGTGGATTCTGATTCGCACTGCGCCGTGTGTAAAGAAGCCTTTGAAATCGGAACTGATGCCCGAGAAATGCCGTGTAAGCATATATATCACTCCGAATGCATAATCCCTTGGCTTTCCATGAGAAATTCCTGTCCGGTCTGCCGGCACGAACTGCCCTCGGAGCGTGTGTCTCCGGCTGGTGGGGTTTCAGATCGAGTTGTTGACGAAGAAACCGTAGGGTTAACGATTTGGAGGCTTCCCGGTGGTGGGTTTGCCGTCGGACGATTCTCCGGCGGAAGATTGGCCGGCGAGAGAGACCCACCGGCTGTGTACACCGAAATGGACGGTGGGTTCAATGGGAACGGAGTTCCAAGGAGGGTTTCGTGGGCTTCGAGAAGAAGCAGGGGAAGAGAGAGCCGAGGAATTGGACGAACATTTCGGAACATATTTTCCTTCTTTGGGAGATTTAGGTCGTCGAGTTCATCTTCTTCCTCGCCTTCAAATGAATCTGAGTCTGTAAGTAGAAGCCATAGCCATTCGAGTTCTTCAGTTTTCAGCAGATACTTACGAAGGCAAAACAGAGCTTGGGTGTTGGAGAACCAGAATGAAAATGGCAGATGGTGA

Protein sequence

MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHTVDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVDSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSWASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYLRRQNRAWVLENQNENGRW*

Homology

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BLAST of Chy11G197940 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.4e-84
Identity = 194/402 (48.26%), Postives = 244/402 (60.70%), Query Frame = 0


Query: 5   TASYWCYRCNRFVRAWAQD------SITCPYCDGGFVEAIETASS------LPASNLHRR 64
           TASYWCY C RFV  WA        S+ CP+CDGGF+E I  +SS      +PAS   R 
Sbjct: 15  TASYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATELTIPASTEVR- 74

Query: 65  LSPSAIHTVDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSA------DAGDVVGGGE 124
                       S  + R S  R RRR G R +FNPV+VL+G A      + GD     +
Sbjct: 75  ------------SINNNRRSVIR-RRRSGRRPSFNPVIVLQGGAGEREEGEEGD--AARD 134

Query: 125 RNSFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEIN--GFGRSENPPASKA 184
           R +F+ YYDDG+GSGLRP+P ++SE LMG+GF+RLLEQL+Q+E +  G GRS NPPASK+
Sbjct: 135 RRAFEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKS 194

Query: 185 AVESMPTIEILESHVDSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPV 244
           A+ES+P +EI + H+ S+++CAVC E FE  T+AREMPCKH++H +CI+PWLS+RNSCPV
Sbjct: 195 AIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPV 254

Query: 245 CRHELPSERVSPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTE 304
           CR ELPSE        S+   ++  VG+TIWRLPGGGFAVGRF+     GER  P V TE
Sbjct: 255 CRFELPSE----PNRRSNNNEEDNAVGMTIWRLPGGGFAVGRFNAAMRDGERVLPVVLTE 314

Query: 305 MDGGFNGNGV--PRRVSWASRRSRG---RESRGIG----------RTFRNIFSFFGRF-- 363
           MDGG  GN    PRR+SW   RS G    +S G G          R  R + S   R   
Sbjct: 315 MDGGGIGNSQDGPRRISWV--RSHGTLESDSNGTGSGSGSGGRLRRMVRGMVSLMRRVRP 374

[Close]

BLAST of Chy11G197940 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.7e-83
Identity = 180/386 (46.63%), Postives = 236/386 (61.14%), Query Frame = 0


Query: 2   STGTASYWCYRCNRFVRAW----AQDSITCPYCDGGFVEAIE-----TASSLPASNLHRR 61
           +T T SYWCY C RF+  W    A   + CPYC+GGF+E IE     T +++PAS     
Sbjct: 25  TTLTTSYWCYSCTRFISVWEDQDANAGVLCPYCNGGFIEEIEDSSNSTVAAIPAS----- 84

Query: 62  LSPSAIHTVDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAG------DVVGGG- 121
                  T +  S +    S  R RRR   R++FNPV+VL G    G      +  G G 
Sbjct: 85  -------TPEVRSVEETHRSIIR-RRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGA 144

Query: 122 --ERNSFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEI--NGFGRSENPPA 181
             ER +++ YYDDG+GSGLRP+P ++SE LMG+GF+RLLEQL+Q+E   NG GRS NPPA
Sbjct: 145 TRERRAYEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPA 204

Query: 182 SKAAVESMPTIEILESHVDSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNS 241
           SK+A+ES+P +EI + H  ++++CAVC E FE G + REMPCKHI+H +CI+PWLS+RNS
Sbjct: 205 SKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNS 264

Query: 242 CPVCRHELPSERVSPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAV 301
           CPVCR ELPS+ +  +        +E  VG+TIWRLPGGGFAVGRF+ G   GER  P V
Sbjct: 265 CPVCRFELPSDPIQRSN------EEEHAVGMTIWRLPGGGFAVGRFNAGVREGERILPVV 324

Query: 302 YTEMD-GGFNGNGVPRRVSWA-------SRRSRGRESRG--IGRTFRNIFSFFGRFRSSS 356
            TEMD GG   N  PRR+SW          R+ GR   G  + R  R + SF  R R S 
Sbjct: 325 LTEMDGGGLGSNEGPRRISWVRAHETPEMSRNGGRSGNGGRLRRAVRGMVSFMRRVRPSR 384

[Close]

BLAST of Chy11G197940 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 9.6e-81
Identity = 184/399 (46.12%), Postives = 238/399 (59.65%), Query Frame = 0


Query: 5   TASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIE------------TASSLPASNLHRR 64
           + SYWCY C+RFV  W  DSI+CP CDGGF+E I+            T ++    +  R 
Sbjct: 3   SGSYWCYSCSRFV--WVSDSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPTRF 62

Query: 65  LSPSAIHTVDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADA--GDVVGGG-ERNS 124
             PS+  +    S  +    T     R     + NPV+VLRGSA A   DVV  G +R++
Sbjct: 63  PPPSSSSSTPSASMHADNSPTPTIVTRTRSNRSPNPVIVLRGSAAAPSSDVVSEGLDRSA 122

Query: 125 FDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEIN---GFGRSENPPASKAAV 184
           F +YYDDG  SGLRP+P +M+EFL+G+GFDRLL+Q++Q+E+N        E+PPASK+A+
Sbjct: 123 FQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHPPASKSAI 182

Query: 185 ESMPTIEILESHV--DSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPV 244
           E++P IEI  +H+  DS SHCAVCKE F + + AREMPC HIYH +CI+PWL++RNSCPV
Sbjct: 183 EALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLAIRNSCPV 242

Query: 245 CRHELPSERVSPAGGVSDRVV-------DEETVGLTIWRLPGGGFAVGRFSGGRLAGERD 304
           CRHELP+E ++   G +   V       ++   GLTIWRLPGGGFAVGR  GG   G+R 
Sbjct: 243 CRHELPAEDLTDGTGAALTAVTATAEEEEDSAAGLTIWRLPGGGFAVGRIPGGWRGGDRM 302

Query: 305 PPAVYTEMDGGFNGN-GVPRRVSWASRR----SRGRESRGIGRTFRNIFSFFGRFRSSSS 364
            P VYTE+DGG  G+  +PRRV+W SRR      G   RG G   R I   FG F  SS 
Sbjct: 303 MPVVYTEVDGGRLGDERLPRRVAWGSRRGGRDGGGSRERGGGFAGR-IMRLFGCFSGSSG 362

[Close]

BLAST of Chy11G197940 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.7e-37
Identity = 90/242 (37.19%), Postives = 137/242 (56.61%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MS+   ++WC+RC R V   A+D++ C YC GGFVE I+   S    ++ R   P+    
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAV-CSYCGGGFVEEIDIGPSRAHRDVER--DPTFDLM 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDR---------STFNPVVVLRGSADAGDVVGGGERNSFD 120
               +F   RL+ R   R +  R         S   P+++  G A     + GG+ +S +
Sbjct: 61  EAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFR--LAGGDNSSVE 120

Query: 121 IYYDDGAGSGLRPVPAT-MSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMP 180
            +  +GA  G+     T   ++  G G + L+EQL+    +G      PPA K++++++P
Sbjct: 121 AFV-NGAAPGIGIARGTNAGDYFFGPGLEELIEQLS----SGTHHRGPPPAPKSSIDALP 180

Query: 181 TIEILESHV-DSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHEL 232
           TI+I + H+  SDSHC VCK+ FE+ ++A++MPC HIYHS+CI+PWL   NSCPVCR EL
Sbjct: 181 TIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKEL 232

[Close]

BLAST of Chy11G197940 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.0e-33
Identity = 117/378 (30.95%), Postives = 169/378 (44.71%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPAS--NLHRRL--SPS 60
           MS+   ++WC+RC R VR   Q+ + C YC GGFVE ++ A + P      HR +     
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPV-CFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQ 60

Query: 61  AIHTVDQDS-FQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGGE-------RN 120
               +D  S F   RL+ R   R +  R+  +      G A    ++ GG+        N
Sbjct: 61  TFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPL--LIFGGQVPYRLTGDN 120

Query: 121 SFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVES 180
           + +  + +G   G+        ++  G G + L EQL+     G  R   PPA ++A+++
Sbjct: 121 AVEALF-NGGSPGIGITRGNTGDYFFGPGLEELFEQLSA----GTTRRGPPPAPRSAIDA 180

Query: 181 MPTIEILESHV-DSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRH 240
           +PTI+I + H+  SDS+C VCK+ FE+G++A++MPC HIYHS+CI+PWL   NSCPVCR 
Sbjct: 181 LPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQ 240

Query: 241 ELPSERVSPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDG 300
           ELPS     A G S                                              
Sbjct: 241 ELPS-----ASGPSSS-------------------------------------------- 300

Query: 301 GFNGNGVPRRVSWASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSN-----ESE 360
                  P R   +S  S    SR  G   RN FS F  FRSS SSSSS  N      S+
Sbjct: 301 --QNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNSD 319

[Close]

BLAST of Chy11G197940 vs. ExPASy TrEMBL
Match: A0A0A0KE74 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G190250 PE=4 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 4.0e-215
Identity = 374/378 (98.94%), Postives = 376/378 (99.47%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLP SNLHRRLSPSAIHT
Sbjct: 39  MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPPSNLHRRLSPSAIHT 98

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120
           +DQDSFQSPRLSTRR RRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS
Sbjct: 99  LDQDSFQSPRLSTRRSRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 158

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD
Sbjct: 159 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 218

Query: 181 SDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 240
           SDSHCAVCKEAFEIGT+AREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV
Sbjct: 219 SDSHCAVCKEAFEIGTEAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 278

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW
Sbjct: 279 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 338

Query: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360
           ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL
Sbjct: 339 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 398

Query: 361 RRQNRAWVLENQNENGRW 379
           RRQNRAWVLENQNENGRW
Sbjct: 399 RRQNRAWVLENQNENGRW 416

[Close]

BLAST of Chy11G197940 vs. ExPASy TrEMBL
Match: A0A5A7V7N5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00040 PE=4 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 4.9e-213
Identity = 370/378 (97.88%), Postives = 374/378 (98.94%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MSTGTASYWCYRC+RFVRAWAQDSITCPYCDGGFVEAIETASSLPA NLHRRLSP+AIHT
Sbjct: 1   MSTGTASYWCYRCSRFVRAWAQDSITCPYCDGGFVEAIETASSLPAPNLHRRLSPTAIHT 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120
           VDQDSFQSPRLSTRR RRR GDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS
Sbjct: 61  VDQDSFQSPRLSTRRSRRRFGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180

Query: 181 SDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 240
           SDSHCAVCKEAFEIGT+AREMPCKHIYHS+CIIPWLSMRNSCPVCRHELPSERVSPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERVSPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFN NGVPRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGVPRRVSW 300

Query: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360
           ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL
Sbjct: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360

Query: 361 RRQNRAWVLENQNENGRW 379
           RRQNRAWVLENQNENGRW
Sbjct: 361 RRQNRAWVLENQNENGRW 378

[Close]

BLAST of Chy11G197940 vs. ExPASy TrEMBL
Match: A0A1S3CKQ2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103502070 PE=4 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 4.9e-213
Identity = 370/378 (97.88%), Postives = 374/378 (98.94%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MSTGTASYWCYRC+RFVRAWAQDSITCPYCDGGFVEAIETASSLPA NLHRRLSP+AIHT
Sbjct: 1   MSTGTASYWCYRCSRFVRAWAQDSITCPYCDGGFVEAIETASSLPAPNLHRRLSPTAIHT 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120
           VDQDSFQSPRLSTRR RRR GDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS
Sbjct: 61  VDQDSFQSPRLSTRRSRRRFGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180

Query: 181 SDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 240
           SDSHCAVCKEAFEIGT+AREMPCKHIYHS+CIIPWLSMRNSCPVCRHELPSERVSPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERVSPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFN NGVPRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGVPRRVSW 300

Query: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360
           ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL
Sbjct: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360

Query: 361 RRQNRAWVLENQNENGRW 379
           RRQNRAWVLENQNENGRW
Sbjct: 361 RRQNRAWVLENQNENGRW 378

[Close]

BLAST of Chy11G197940 vs. ExPASy TrEMBL
Match: A0A6J1G610 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111451192 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 2.9e-197
Identity = 346/379 (91.29%), Postives = 358/379 (94.46%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           M++GTASYWCYRCNRFVRAW QD+I+CPYCDGGFVEAIETASSLPA+NLHRR SPSAIHT
Sbjct: 1   MASGTASYWCYRCNRFVRAWVQDTISCPYCDGGFVEAIETASSLPAANLHRRFSPSAIHT 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDV-VGGGERNSFDIYYDDGAG 120
           +DQD FQSPRLSTRR RRRLGDRSTFNPVVVLRGSAD GD   GGGE NSFDIYYDDGAG
Sbjct: 61  LDQDPFQSPRLSTRRSRRRLGDRSTFNPVVVLRGSADGGDEGGGGGENNSFDIYYDDGAG 120

Query: 121 SGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHV 180
           SGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHV
Sbjct: 121 SGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHV 180

Query: 181 DSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGG 240
           DSDSHCAVCKEAFEIGT+AREMPCKHIYHS+CIIPWLSMRNSCPVCRHELPSERVSPAGG
Sbjct: 181 DSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERVSPAGG 240

Query: 241 VSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVS 300
           VSDRVVDEETVGLTIWRLPGGGFAVGRF+GGRL GERDPPAVYTEMDGGFN NG PRRVS
Sbjct: 241 VSDRVVDEETVGLTIWRLPGGGFAVGRFTGGRLTGERDPPAVYTEMDGGFNANGAPRRVS 300

Query: 301 WASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRY 360
           WASRRSRGRE+RGIGRTFRNIFSFFGR RSS+SSSS  SNE+ESVSRSHS SSSSVFSRY
Sbjct: 301 WASRRSRGRENRGIGRTFRNIFSFFGRLRSSNSSSSLASNETESVSRSHSRSSSSVFSRY 360

Query: 361 LRRQNRAWVLENQNENGRW 379
           LRR NR W LEN  ENGRW
Sbjct: 361 LRRPNRTW-LENHTENGRW 378

[Close]

BLAST of Chy11G197940 vs. ExPASy TrEMBL
Match: A0A6J1L6T2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499706 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 6.5e-197
Identity = 346/379 (91.29%), Postives = 358/379 (94.46%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           M++GTASYWCYRCNRFVRAW QD+I+CPYCDGGFVEAIETASSLPA+NLHRR SPSAIHT
Sbjct: 1   MASGTASYWCYRCNRFVRAWVQDTISCPYCDGGFVEAIETASSLPAANLHRRFSPSAIHT 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDV-VGGGERNSFDIYYDDGAG 120
           +DQD FQSPRLSTRR RRRLGDRSTFNPVVVLRGSAD GD   GGGE NSFDIYYDDGAG
Sbjct: 61  LDQDPFQSPRLSTRRSRRRLGDRSTFNPVVVLRGSADGGDEGGGGGENNSFDIYYDDGAG 120

Query: 121 SGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHV 180
           SGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHV
Sbjct: 121 SGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHV 180

Query: 181 DSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGG 240
           DSDSHCAVCKEAF IGT+AREMPCKHIYHS+CIIPWLSMRNSCPVCRHELPSERVSPAGG
Sbjct: 181 DSDSHCAVCKEAFVIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERVSPAGG 240

Query: 241 VSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVS 300
           VSDRVVDEETVGLTIWRLPGGGFAVGRF+GGRL GERDPPAVYTEMDGGFN NGVPRRVS
Sbjct: 241 VSDRVVDEETVGLTIWRLPGGGFAVGRFTGGRLTGERDPPAVYTEMDGGFNANGVPRRVS 300

Query: 301 WASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRY 360
           WASRRSRGRE+RGIGRTFRNIFSFFGR RSS+SSSS  SNE+ESVSRSHS SSSSVFSRY
Sbjct: 301 WASRRSRGRENRGIGRTFRNIFSFFGRLRSSNSSSSLASNETESVSRSHSRSSSSVFSRY 360

Query: 361 LRRQNRAWVLENQNENGRW 379
           LRR NR W LEN  ENGRW
Sbjct: 361 LRRPNRTW-LENHTENGRW 378

[Close]

BLAST of Chy11G197940 vs. NCBI nr
Match: KAE8646961.1 (hypothetical protein Csa_020907 [Cucumis sativus])

HSP 1 Score: 748 bits (1930), Expect = 4.07e-272
Identity = 374/378 (98.94%), Postives = 376/378 (99.47%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLP SNLHRRLSPSAIHT
Sbjct: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPPSNLHRRLSPSAIHT 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120
           +DQDSFQSPRLSTRR RRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS
Sbjct: 61  LDQDSFQSPRLSTRRSRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180

Query: 181 SDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 240
           SDSHCAVCKEAFEIGT+AREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 300

Query: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360
           ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL
Sbjct: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360

Query: 361 RRQNRAWVLENQNENGRW 378
           RRQNRAWVLENQNENGRW
Sbjct: 361 RRQNRAWVLENQNENGRW 378

[Close]

BLAST of Chy11G197940 vs. NCBI nr
Match: XP_004143127.2 (E3 ubiquitin-protein ligase RDUF1, partial [Cucumis sativus])

HSP 1 Score: 748 bits (1930), Expect = 1.58e-271
Identity = 374/378 (98.94%), Postives = 376/378 (99.47%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLP SNLHRRLSPSAIHT
Sbjct: 37  MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPPSNLHRRLSPSAIHT 96

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120
           +DQDSFQSPRLSTRR RRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS
Sbjct: 97  LDQDSFQSPRLSTRRSRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 156

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD
Sbjct: 157 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 216

Query: 181 SDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 240
           SDSHCAVCKEAFEIGT+AREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV
Sbjct: 217 SDSHCAVCKEAFEIGTEAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 276

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW
Sbjct: 277 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 336

Query: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360
           ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL
Sbjct: 337 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 396

Query: 361 RRQNRAWVLENQNENGRW 378
           RRQNRAWVLENQNENGRW
Sbjct: 397 RRQNRAWVLENQNENGRW 414

[Close]

BLAST of Chy11G197940 vs. NCBI nr
Match: XP_008464108.1 (PREDICTED: E3 ubiquitin-protein ligase RING1 [Cucumis melo] >KAA0061885.1 E3 ubiquitin-protein ligase RING1 [Cucumis melo var. makuwa] >TYK15372.1 E3 ubiquitin-protein ligase RING1 [Cucumis melo var. makuwa])

HSP 1 Score: 741 bits (1912), Expect = 2.26e-269
Identity = 370/378 (97.88%), Postives = 374/378 (98.94%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MSTGTASYWCYRC+RFVRAWAQDSITCPYCDGGFVEAIETASSLPA NLHRRLSP+AIHT
Sbjct: 1   MSTGTASYWCYRCSRFVRAWAQDSITCPYCDGGFVEAIETASSLPAPNLHRRLSPTAIHT 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120
           VDQDSFQSPRLSTRR RRR GDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS
Sbjct: 61  VDQDSFQSPRLSTRRSRRRFGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180

Query: 181 SDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 240
           SDSHCAVCKEAFEIGT+AREMPCKHIYHS+CIIPWLSMRNSCPVCRHELPSERVSPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERVSPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFN NGVPRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGVPRRVSW 300

Query: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360
           ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL
Sbjct: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360

Query: 361 RRQNRAWVLENQNENGRW 378
           RRQNRAWVLENQNENGRW
Sbjct: 361 RRQNRAWVLENQNENGRW 378

[Close]

BLAST of Chy11G197940 vs. NCBI nr
Match: XP_038902866.1 (E3 ubiquitin-protein ligase RDUF1 [Benincasa hispida])

HSP 1 Score: 728 bits (1880), Expect = 1.70e-264
Identity = 362/378 (95.77%), Postives = 370/378 (97.88%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MS+GTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPA+NLHRR SPS IHT
Sbjct: 1   MSSGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPAANLHRRFSPSTIHT 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120
           +DQDSFQSPRLSTRR RRRLGDRSTFNPVVVLRGSAD GDVVGGGERNSFDIYYDDGAGS
Sbjct: 61  LDQDSFQSPRLSTRRSRRRLGDRSTFNPVVVLRGSADGGDVVGGGERNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180

Query: 181 SDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 240
           SDSHCAVCKEAFEIGT+AREMPCKHIYHS+CIIPWLSMRNSCPVCRHELPSERVSPAG V
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERVSPAGSV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRF+GGRLAGERD PAVYTEMDGGFN NG PRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFAGGRLAGERDTPAVYTEMDGGFNANGAPRRVSW 300

Query: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRYL 360
           ASRRSRGRESRGIGRTFRNIFSFFGRFRSS+SSSSSPSN+SESVSRSHSHSSSSVFSRYL
Sbjct: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSTSSSSSPSNDSESVSRSHSHSSSSVFSRYL 360

Query: 361 RRQNRAWVLENQNENGRW 378
           RRQNRAWVLENQNENGRW
Sbjct: 361 RRQNRAWVLENQNENGRW 378

[Close]

BLAST of Chy11G197940 vs. NCBI nr
Match: XP_023532253.1 (E3 ubiquitin-protein ligase RDUF2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 689 bits (1777), Expect = 8.46e-249
Identity = 346/379 (91.29%), Postives = 359/379 (94.72%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           M++GTASYWCYRCNRFVRAW QD+I+CPYCDGGFVEAIETASSLPA+NLHRR SPSAIHT
Sbjct: 1   MASGTASYWCYRCNRFVRAWVQDTISCPYCDGGFVEAIETASSLPAANLHRRFSPSAIHT 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAGDVVGGG-ERNSFDIYYDDGAG 120
           +DQD FQSPRLSTRR RRRLGDRSTFNPVVVLRGSAD GD  GGG E NSFDIYYDDGAG
Sbjct: 61  LDQDPFQSPRLSTRRSRRRLGDRSTFNPVVVLRGSADGGDEGGGGGENNSFDIYYDDGAG 120

Query: 121 SGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHV 180
           SGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHV
Sbjct: 121 SGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHV 180

Query: 181 DSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGG 240
           DSDSHCAVCKEAFEIGT+AREMPCKHIYHS+CIIPWLSMRNSCPVCRHELPSERVSPAGG
Sbjct: 181 DSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERVSPAGG 240

Query: 241 VSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVS 300
           VSDRVVDEETVGLTIWRLPGGGFAVGRF+GGRL GERDPPAVYTEMDGGFN NG PRRVS
Sbjct: 241 VSDRVVDEETVGLTIWRLPGGGFAVGRFTGGRLTGERDPPAVYTEMDGGFNANGAPRRVS 300

Query: 301 WASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHSSSSVFSRY 360
           WASRRSRGRE+RGIGRTFRNIFSFFGR RSS+SSSS  SNE+ESVSRSHS SSSSVFSRY
Sbjct: 301 WASRRSRGRENRGIGRTFRNIFSFFGRLRSSNSSSSLASNETESVSRSHSRSSSSVFSRY 360

Query: 361 LRRQNRAWVLENQNENGRW 378
           LRR NR+W LEN  ENGRW
Sbjct: 361 LRRPNRSW-LENHTENGRW 378

[Close]

BLAST of Chy11G197940 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 314.7 bits (805), Expect = 1.0e-85
Identity = 194/402 (48.26%), Postives = 244/402 (60.70%), Query Frame = 0


Query: 5   TASYWCYRCNRFVRAWAQD------SITCPYCDGGFVEAIETASS------LPASNLHRR 64
           TASYWCY C RFV  WA        S+ CP+CDGGF+E I  +SS      +PAS   R 
Sbjct: 15  TASYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATELTIPASTEVR- 74

Query: 65  LSPSAIHTVDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSA------DAGDVVGGGE 124
                       S  + R S  R RRR G R +FNPV+VL+G A      + GD     +
Sbjct: 75  ------------SINNNRRSVIR-RRRSGRRPSFNPVIVLQGGAGEREEGEEGD--AARD 134

Query: 125 RNSFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEIN--GFGRSENPPASKA 184
           R +F+ YYDDG+GSGLRP+P ++SE LMG+GF+RLLEQL+Q+E +  G GRS NPPASK+
Sbjct: 135 RRAFEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKS 194

Query: 185 AVESMPTIEILESHVDSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPV 244
           A+ES+P +EI + H+ S+++CAVC E FE  T+AREMPCKH++H +CI+PWLS+RNSCPV
Sbjct: 195 AIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPV 254

Query: 245 CRHELPSERVSPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTE 304
           CR ELPSE        S+   ++  VG+TIWRLPGGGFAVGRF+     GER  P V TE
Sbjct: 255 CRFELPSE----PNRRSNNNEEDNAVGMTIWRLPGGGFAVGRFNAAMRDGERVLPVVLTE 314

Query: 305 MDGGFNGNGV--PRRVSWASRRSRG---RESRGIG----------RTFRNIFSFFGRF-- 363
           MDGG  GN    PRR+SW   RS G    +S G G          R  R + S   R   
Sbjct: 315 MDGGGIGNSQDGPRRISWV--RSHGTLESDSNGTGSGSGSGGRLRRMVRGMVSLMRRVRP 374

[Close]

BLAST of Chy11G197940 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 310.5 bits (794), Expect = 1.9e-84
Identity = 180/386 (46.63%), Postives = 236/386 (61.14%), Query Frame = 0


Query: 2   STGTASYWCYRCNRFVRAW----AQDSITCPYCDGGFVEAIE-----TASSLPASNLHRR 61
           +T T SYWCY C RF+  W    A   + CPYC+GGF+E IE     T +++PAS     
Sbjct: 25  TTLTTSYWCYSCTRFISVWEDQDANAGVLCPYCNGGFIEEIEDSSNSTVAAIPAS----- 84

Query: 62  LSPSAIHTVDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADAG------DVVGGG- 121
                  T +  S +    S  R RRR   R++FNPV+VL G    G      +  G G 
Sbjct: 85  -------TPEVRSVEETHRSIIR-RRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGA 144

Query: 122 --ERNSFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEI--NGFGRSENPPA 181
             ER +++ YYDDG+GSGLRP+P ++SE LMG+GF+RLLEQL+Q+E   NG GRS NPPA
Sbjct: 145 TRERRAYEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPA 204

Query: 182 SKAAVESMPTIEILESHVDSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNS 241
           SK+A+ES+P +EI + H  ++++CAVC E FE G + REMPCKHI+H +CI+PWLS+RNS
Sbjct: 205 SKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNS 264

Query: 242 CPVCRHELPSERVSPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAV 301
           CPVCR ELPS+ +  +        +E  VG+TIWRLPGGGFAVGRF+ G   GER  P V
Sbjct: 265 CPVCRFELPSDPIQRSN------EEEHAVGMTIWRLPGGGFAVGRFNAGVREGERILPVV 324

Query: 302 YTEMD-GGFNGNGVPRRVSWA-------SRRSRGRESRG--IGRTFRNIFSFFGRFRSSS 356
            TEMD GG   N  PRR+SW          R+ GR   G  + R  R + SF  R R S 
Sbjct: 325 LTEMDGGGLGSNEGPRRISWVRAHETPEMSRNGGRSGNGGRLRRAVRGMVSFMRRVRPSR 384

[Close]

BLAST of Chy11G197940 vs. TAIR 10
Match: AT2G39720.1 (RING-H2 finger C2A )

HSP 1 Score: 302.0 bits (772), Expect = 6.8e-82
Identity = 184/399 (46.12%), Postives = 238/399 (59.65%), Query Frame = 0


Query: 5   TASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIE------------TASSLPASNLHRR 64
           + SYWCY C+RFV  W  DSI+CP CDGGF+E I+            T ++    +  R 
Sbjct: 3   SGSYWCYSCSRFV--WVSDSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPTRF 62

Query: 65  LSPSAIHTVDQDSFQSPRLSTRRGRRRLGDRSTFNPVVVLRGSADA--GDVVGGG-ERNS 124
             PS+  +    S  +    T     R     + NPV+VLRGSA A   DVV  G +R++
Sbjct: 63  PPPSSSSSTPSASMHADNSPTPTIVTRTRSNRSPNPVIVLRGSAAAPSSDVVSEGLDRSA 122

Query: 125 FDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEIN---GFGRSENPPASKAAV 184
           F +YYDDG  SGLRP+P +M+EFL+G+GFDRLL+Q++Q+E+N        E+PPASK+A+
Sbjct: 123 FQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHPPASKSAI 182

Query: 185 ESMPTIEILESHV--DSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPV 244
           E++P IEI  +H+  DS SHCAVCKE F + + AREMPC HIYH +CI+PWL++RNSCPV
Sbjct: 183 EALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLAIRNSCPV 242

Query: 245 CRHELPSERVSPAGGVSDRVV-------DEETVGLTIWRLPGGGFAVGRFSGGRLAGERD 304
           CRHELP+E ++   G +   V       ++   GLTIWRLPGGGFAVGR  GG   G+R 
Sbjct: 243 CRHELPAEDLTDGTGAALTAVTATAEEEEDSAAGLTIWRLPGGGFAVGRIPGGWRGGDRM 302

Query: 305 PPAVYTEMDGGFNGN-GVPRRVSWASRR----SRGRESRGIGRTFRNIFSFFGRFRSSSS 364
            P VYTE+DGG  G+  +PRRV+W SRR      G   RG G   R I   FG F  SS 
Sbjct: 303 MPVVYTEVDGGRLGDERLPRRVAWGSRRGGRDGGGSRERGGGFAGR-IMRLFGCFSGSSG 362

[Close]

BLAST of Chy11G197940 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 158.3 bits (399), Expect = 1.2e-38
Identity = 90/242 (37.19%), Postives = 137/242 (56.61%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MS+   ++WC+RC R V   A+D++ C YC GGFVE I+   S    ++ R   P+    
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAV-CSYCGGGFVEEIDIGPSRAHRDVER--DPTFDLM 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDR---------STFNPVVVLRGSADAGDVVGGGERNSFD 120
               +F   RL+ R   R +  R         S   P+++  G A     + GG+ +S +
Sbjct: 61  EAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFR--LAGGDNSSVE 120

Query: 121 IYYDDGAGSGLRPVPAT-MSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMP 180
            +  +GA  G+     T   ++  G G + L+EQL+    +G      PPA K++++++P
Sbjct: 121 AFV-NGAAPGIGIARGTNAGDYFFGPGLEELIEQLS----SGTHHRGPPPAPKSSIDALP 180

Query: 181 TIEILESHV-DSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHEL 232
           TI+I + H+  SDSHC VCK+ FE+ ++A++MPC HIYHS+CI+PWL   NSCPVCR EL
Sbjct: 181 TIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKEL 232

[Close]

BLAST of Chy11G197940 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 158.3 bits (399), Expect = 1.2e-38
Identity = 90/242 (37.19%), Postives = 137/242 (56.61%), Query Frame = 0


Query: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPASNLHRRLSPSAIHT 60
           MS+   ++WC+RC R V   A+D++ C YC GGFVE I+   S    ++ R   P+    
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAV-CSYCGGGFVEEIDIGPSRAHRDVER--DPTFDLM 60

Query: 61  VDQDSFQSPRLSTRRGRRRLGDR---------STFNPVVVLRGSADAGDVVGGGERNSFD 120
               +F   RL+ R   R +  R         S   P+++  G A     + GG+ +S +
Sbjct: 61  EAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFR--LAGGDNSSVE 120

Query: 121 IYYDDGAGSGLRPVPAT-MSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMP 180
            +  +GA  G+     T   ++  G G + L+EQL+    +G      PPA K++++++P
Sbjct: 121 AFV-NGAAPGIGIARGTNAGDYFFGPGLEELIEQLS----SGTHHRGPPPAPKSSIDALP 180

Query: 181 TIEILESHV-DSDSHCAVCKEAFEIGTDAREMPCKHIYHSECIIPWLSMRNSCPVCRHEL 232
           TI+I + H+  SDSHC VCK+ FE+ ++A++MPC HIYHS+CI+PWL   NSCPVCR EL
Sbjct: 181 TIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKEL 232

The following BLAST results are available for this feature:

BLAST of Chy11G197940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940T5	1.4e-84	48.26	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q9SNB6	2.7e-83	46.63	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
O22283	9.6e-81	46.12	Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	1.7e-37	37.19	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
O22197	2.0e-33	30.95	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of Chy11G197940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KE74	4.0e-215	98.94	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G190250 PE...	[more]
A0A5A7V7N5	4.9e-213	97.88	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3CKQ2	4.9e-213	97.88	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103502070 PE=4 ...	[more]
A0A6J1G610	2.9e-197	91.29	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111451192...	[more]
A0A6J1L6T2	6.5e-197	91.29	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499706 P...	[more]

BLAST of Chy11G197940 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8646961.1	4.07e-272	98.94	hypothetical protein Csa_020907 [Cucumis sativus]	[more]
XP_004143127.2	1.58e-271	98.94	E3 ubiquitin-protein ligase RDUF1, partial [Cucumis sativus]	[more]
XP_008464108.1	2.26e-269	97.88	PREDICTED: E3 ubiquitin-protein ligase RING1 [Cucumis melo] >KAA0061885.1 E3 ubi...	[more]
XP_038902866.1	1.70e-264	95.77	E3 ubiquitin-protein ligase RDUF1 [Benincasa hispida]	[more]
XP_023532253.1	8.46e-249	91.29	E3 ubiquitin-protein ligase RDUF2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy11G197940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59550.1	1.0e-85	48.26	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G46620.1	1.9e-84	46.63	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G39720.1	6.8e-82	46.12	RING-H2 finger C2A	[more]
AT3G56580.2	1.2e-38	37.19	RING/U-box superfamily protein	[more]
AT3G56580.1	1.2e-38	37.19	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 185..225 e-value: 3.0E-7 score: 40.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 184..226 e-value: 9.0E-11 score: 42.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 185..226 score: 12.164712
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 7..38 e-value: 3.8E-14 score: 52.7
IPR010543	Domain of unknown function DUF1117	PFAM	PF06547	DUF1117	coord: 250..361 e-value: 2.7E-41 score: 140.7
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 151..232 e-value: 3.7E-21 score: 76.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 328..353
None	No IPR available	PANTHER	PTHR15710:SF24	OS05G0110000 PROTEIN	coord: 4..315
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 4..315
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 185..226 e-value: 2.30153E-23 score: 89.6844
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 165..231
IPR000253	Forkhead-associated (FHA) domain	PROSITE	PS50006	FHA_DOMAIN	coord: 322..378 score: 7.2427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy11G197940.1	Chy11G197940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0061630	ubiquitin protein ligase activity