Chy11G191270 (gene) Cucumber (hystrix) v1

Overview
NameChy11G191270
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptiontRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
LocationchrH11: 6038955 .. 6043023 (-)
RNA-Seq ExpressionChy11G191270
SyntenyChy11G191270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATCTCTTTCCAATTCTCATCTTCATCTCAAATCTATATTCTCTCTCCCTAACTCTCTCATGGCTTCCTCTCTTACACAAACCCTTAAACCAGATTTACCTCCTCAAGACCTGGACGACCTTCTCTGCTCCCCTTCACAGAAGCAGCCACAACAGGAATCCTCCTCTCTTACCAATTCCAATTCCAATGACTCAACTCCTCTCTTGGGGTCGCCGACTGGATATTTGAGTCGCGAGTCGCGCATTGAACGAGCCTGGGTTCACTGGACCAAGCTCGGTCGCCCAAAGTTGATTGTAGCTCCCATGGTTGATAATTCGGAGCTGCCATTTCGTTTACTCTGCAGGAAATATGGTGCTGAGGCCGCGTATACGCCTATGCTCCATTCACGAATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTCACCACTTGTCAGGTATGTTACAATTTAAAGCCATTTTATAGTGTTAAAAACTTTAATATTCTGTTTCATTTGGTATTCATGTATGTTTCCGGCTGCTATTTGTGGATCAGTAGCTAATTGTAGGTTTTAGTCTTCTCTGTACTATTGTTTAAGTTTATTTTTCATTGTTTTTCAATTTTTGAATGCTTTGTAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTCTGTTGGAGGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTACTCTCTATTACTCTAATTTGTACTCGGTTGATTAATTCGTATGGATGTTGGCATTCTTTATGGTCGTATGTTCCAAATGCTGACTTCTTATACTTCTCGTCGGTGGTGGAGTAAGGATTTGTTCTCCAAAAGACTTTATGTTTGTTAAGATTGTGGATTGTTCACCAAGTACATTTTGTGATTTAGAAGTAACAAAACAAAACTACAAAAGAAGGGAGCCCGCAATAGGAGGATTTACGAAGACTTCTCCAATTAGAGAAAAGGAAAGGAAAGCTATAGTAATGAAAAAGAGGGGAGCATTTACTCCAAGTCACACAAACTAACTATTGTCCCAAAAATATCCTTAAAAAATTTTCTATTACCCTATTGGCAAATGATCCACTAAAGAGCACTCACATAATGTTCCCGCAAAAAGCTTCGTTTCCTTTCTGAATGGATGACCCCTTGAACTTCAACTGTTTAAGCAATGAAGATCTTTGGATCCTCTGGTAGGGCACTATGTCTTGGAACATTTTAAACTATATTATAACTTGATGCATGTGCATGTAAAGGTATCCGAGGGATCTTGATATTGAGGAGTTACCTTCCATTGTGAAAATCCTTGAGGATGTCACTTTATCCCAGACAAAGGTATTTGGAAAATGGGGAAAAGGGCGTCTTTTCAATCAGCTCCCTCTTAAAGATATCCATTCTAATCTAAAATCTTGTCACTAGAGCAAGACGATGAATCATACATCAAGCAGCAATGATCAAGTGTTGAAACCATTAAGAAAATATATTGCTAAAAGACAACAAATGAACAATCAATTCCTAATAGAAAAATAGTTAAGTCAACCTCAATCCACAAGCTCAATTGATCCCTAGAACTAAAATGTTTAGCGACTTATGATCTCACAATACGTGAAAGAAACATTGAGTTTACAAAATCCAGAATTCTAAAAGATCAAGGGAAAAATTAAGAAGGTTGGCTAGTGATTTGATCCCGTTGCCATAATAAAACCACATTCTGTGCTATCGATTCTTGCAATCAAGCTCCCAAGAATCTTCCATCATGATCACACACCTCTATCTCTTTGGAAATCGAAGTTATCCACTATCTCATCCTCCAAAAATCCAGGTTGAATGATCTCCTTAAATCCAAAGTGGGAGTTATATAGCTGATTTGATGATAGCATCGTGATGCTACACTGCATTGTCCTTATCACCTTGCCGGGTGGTGGGATCATGGGCTGATTGAACTTAATAGCTTTGTGTCAAGCCTAGCATTGCACATTGCTGCAACGCTTGTTGGGGGCTTTTTTCTTTATGTTTTCCTCTCTAAGGTTAAAATCGAGCCTAGCATTGCACTCCCTAAGGCTTCTTTTTTTTTTTCTTTCATATTTTCACCTGAATTAATCCTTTTCAGTTCAAATTCTTGGTTCAAATTCAACAAATAAATATTAATAACAAAATAACCAAATTATAAGCCTAAGAAGGTATTTTTTGAGTGCTTTTCAGTTCCGTCAAACATTGTCCATATACACATGTCAATAGTGGCTTGTGGGTCTATCCTTGCACAGAGAGAGATCAAATTTAAGAGGAAATCATCTATGATAGAGGTTCATCCCCAGCCAAAACATCCTAAAAGATAAATCCAAAGCATCCCCAACTATATAGTTCATTCTGTCACGGATGTGATTGGTTTCAAATACAGGCTATTTTGGGTGTAAGAACACCCTTTAGCAATTATAGTCTCTCCTTTTATTTCAAATTGGAGATGTTGTTTTCTAACACACCTATAAGTGGGTAGTTTTTTAGCTTCATTTATTTCAAGTGTTTCTCTTCTAAAAGAACACATTGAAGACCCTTAAATGATTAGAGTGGCTTTTACATATAACTTCTAACTGTAGGAATTAAAATTGGACAAACTTTTTAAAAGAAAATCATTTATGGCTTTTTTATTCTGTGTGTTATTTTCTGCTTTATTCTCCACTAAAGTTGCGTCTAAACATGAGGGAAAAGGAAAGAAAGAATTTTTTTTTTTGTTAGTTTGGGTTGATAATGGTTCAGGTTTTCAAATAAGAAAAACTGAAAACATTCAGCCGGGGCCGAGTTTAGTCAAATTTGAACCAAATATACTCGAATCCGATCAAATATTGAATAGTTTGCGAAGTAATGGAAGTGGTGCACCCGGTTAAAATTTTGGTTCCATATCCTAGAGGGCTCGAGTTTGATCCTCCGTCCTTGAGATTGGATCTTTTTAAAGACATTGGTGTGTTTCCTTCTGTTCAGCGAATTATTTTCAGCACATCCCATTTCCAGTTCACAGGTTCTAAGCCCAACCCACACGCTGTGTGCCATTAACCAAATGGTGTACTGCTTCAAAACACTAAACCAACAGTTTGGTAGGTTTGGTTATTATGTGTGACAATTCAGTTCGTTTTTTAAATTTCTTCCTAGATTTAGCAATTTACTTTTTGATAGTCTAAACTAACAAAAACAATGTAGGATAATCTGAGTTCACTTGCTTTCCAAATGTGTTGCATACCAAATATGTTGATTGTGTTCATATTTTTCACTTAAGGTTCCTGAACATCAATTGAGCAGTGAAGTAATTTTGTGCAGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACTTACCTCTTGTAAAAGCAATAGTAGAGAAACTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATCTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGATGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAAAAATTCCGAGCTAACTGGAGTGCCATTAGTGCGGTTAAAAACGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATACGGCACATGGAAGATGTTAACAACTGTTTACAAGAGACGGGAGTTGAGGGTGTGCTCTCGGCAGAAACCCTTCTGGAGAACCCTGCACTTTTTGCTGGGTTTCGAACTGCTGAATGGATAGAGGGCCACAAAGAAAATACCCGGGATGGTAACCTGGATCAAGCGGATCTGCTTGTAGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCATGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGATTTCAATGCACAATCTAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAATTGCAGGTAAAAATGCCTCTTTATATCAAAGATTCTCATGCACTTGCAATTTCATCCAATGCTTGA

mRNA sequence

ATGAGATCTCTTTCCAATTCTCATCTTCATCTCAAATCTATATTCTCTCTCCCTAACTCTCTCATGGCTTCCTCTCTTACACAAACCCTTAAACCAGATTTACCTCCTCAAGACCTGGACGACCTTCTCTGCTCCCCTTCACAGAAGCAGCCACAACAGGAATCCTCCTCTCTTACCAATTCCAATTCCAATGACTCAACTCCTCTCTTGGGGTCGCCGACTGGATATTTGAGTCGCGAGTCGCGCATTGAACGAGCCTGGGTTCACTGGACCAAGCTCGGTCGCCCAAAGTTGATTGTAGCTCCCATGGTTGATAATTCGGAGCTGCCATTTCGTTTACTCTGCAGGAAATATGGTGCTGAGGCCGCGTATACGCCTATGCTCCATTCACGAATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTCACCACTTGTCAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTCTGTTGGAGGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTATCCGAGGGATCTTGATATTGAGGAGTTACCTTCCATTGTGAAAATCCTTGAGGATGTCACTTTATCCCAGACAAAGGTTCCTGAACATCAATTGAGCAGTGAAGTAATTTTGTGCAGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACTTACCTCTTGTAAAAGCAATAGTAGAGAAACTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATCTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGATGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAAAAATTCCGAGCTAACTGGAGTGCCATTAGTGCGGTTAAAAACGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATACGGCACATGGAAGATGTTAACAACTGTTTACAAGAGACGGGAGTTGAGGGTGTGCTCTCGGCAGAAACCCTTCTGGAGAACCCTGCACTTTTTGCTGGGTTTCGAACTGCTGAATGGATAGAGGGCCACAAAGAAAATACCCGGGATGGTAACCTGGATCAAGCGGATCTGCTTGTAGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCATGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGATTTCAATGCACAATCTAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAATTGCAGGTAAAAATGCCTCTTTATATCAAAGATTCTCATGCACTTGCAATTTCATCCAATGCTTGA

Coding sequence (CDS)

ATGAGATCTCTTTCCAATTCTCATCTTCATCTCAAATCTATATTCTCTCTCCCTAACTCTCTCATGGCTTCCTCTCTTACACAAACCCTTAAACCAGATTTACCTCCTCAAGACCTGGACGACCTTCTCTGCTCCCCTTCACAGAAGCAGCCACAACAGGAATCCTCCTCTCTTACCAATTCCAATTCCAATGACTCAACTCCTCTCTTGGGGTCGCCGACTGGATATTTGAGTCGCGAGTCGCGCATTGAACGAGCCTGGGTTCACTGGACCAAGCTCGGTCGCCCAAAGTTGATTGTAGCTCCCATGGTTGATAATTCGGAGCTGCCATTTCGTTTACTCTGCAGGAAATATGGTGCTGAGGCCGCGTATACGCCTATGCTCCATTCACGAATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTCACCACTTGTCAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTCTGTTGGAGGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTATCCGAGGGATCTTGATATTGAGGAGTTACCTTCCATTGTGAAAATCCTTGAGGATGTCACTTTATCCCAGACAAAGGTTCCTGAACATCAATTGAGCAGTGAAGTAATTTTGTGCAGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACTTACCTCTTGTAAAAGCAATAGTAGAGAAACTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATCTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGATGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAAAAATTCCGAGCTAACTGGAGTGCCATTAGTGCGGTTAAAAACGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATACGGCACATGGAAGATGTTAACAACTGTTTACAAGAGACGGGAGTTGAGGGTGTGCTCTCGGCAGAAACCCTTCTGGAGAACCCTGCACTTTTTGCTGGGTTTCGAACTGCTGAATGGATAGAGGGCCACAAAGAAAATACCCGGGATGGTAACCTGGATCAAGCGGATCTGCTTGTAGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCATGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGATTTCAATGCACAATCTAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAATTGCAGGTAAAAATGCCTCTTTATATCAAAGATTCTCATGCACTTGCAATTTCATCCAATGCTTGA

Protein sequence

MRSLSNSHLHLKSIFSLPNSLMASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTNSNSNDSTPLLGSPTGYLSRESRIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA*
Homology
BLAST of Chy11G191270 vs. ExPASy Swiss-Prot
Match: Q8K582 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 9.1e-73
Identity = 142/338 (42.01%), Postives = 194/338 (57.40%), Query Frame = 0

Query: 90  WTK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 149
           W++ LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRALGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 150 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKILEDVTL 209
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLG                      
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLG---------------------- 129

Query: 210 SQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIR 269
                             CPQ IA+RG YGAFL +   L++ ++      L VPV+CKIR
Sbjct: 130 ------------------CPQMIAKRGRYGAFLQEEWDLLQRMILLAHERLSVPVTCKIR 189

Query: 270 IFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANG 329
           +FP +  T+ YA+MLE AGC LL VHGRT+++K      A+W  I AV+ AV IPV ANG
Sbjct: 190 VFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTASWEHIKAVRKAVGIPVFANG 249

Query: 330 NIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLDQADLL 389
           NIR ++DV  C+Q+TGV+GV+SAE  L NPALF G   A W                +L 
Sbjct: 250 NIRCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAVW----------------ELA 291

Query: 390 VDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 425
            +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 310 DEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

BLAST of Chy11G191270 vs. ExPASy Swiss-Prot
Match: Q8C2P3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.5e-72
Identity = 141/338 (41.72%), Postives = 195/338 (57.69%), Query Frame = 0

Query: 90  WTK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 149
           W++ LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRTLGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 150 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKILEDVTL 209
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLG                      
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLG---------------------- 129

Query: 210 SQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIR 269
                             CPQ IA+RG+YGAFL +   L++ ++      L VPV+CKIR
Sbjct: 130 ------------------CPQMIAKRGHYGAFLQEEWDLLQRMILLAHERLSVPVTCKIR 189

Query: 270 IFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANG 329
           +FP +  T+ YA+MLE AGC LL VHGRT+++K      A+W  I AV+ AV IPV ANG
Sbjct: 190 VFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTASWEHIKAVRKAVGIPVFANG 249

Query: 330 NIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLDQADLL 389
           NI+ ++DV  C+Q+TGV+GV+SAE  L NPALF G   A W                +L 
Sbjct: 250 NIQCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAVW----------------ELA 291

Query: 390 VDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 425
            +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 310 EEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

BLAST of Chy11G191270 vs. ExPASy Swiss-Prot
Match: Q6P1R4 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS1L PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 5.9e-72
Identity = 139/328 (42.38%), Postives = 189/328 (57.62%), Query Frame = 0

Query: 99  IVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTTCQEDRPLFVQ 158
           +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    C EDRPL VQ
Sbjct: 20  VVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCEVCPEDRPLIVQ 79

Query: 159 FCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQL 218
           FCANDP+V ++AA   + YCD +DLNLG                                
Sbjct: 80  FCANDPEVFVQAALLAQDYCDAIDLNLG-------------------------------- 139

Query: 219 SSEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTIN 278
                   CPQ IA+RG+YGAFL D   L++ ++      L VPV+CKIR+FP +  T+ 
Sbjct: 140 --------CPQMIAKRGHYGAFLQDEWDLLQRMILLAHEKLSVPVTCKIRVFPEIDKTVR 199

Query: 279 YARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNN 338
           YA+MLE AGC LL VHGRT+++K      A+W  I AV+ AV IPV ANGNI+ ++DV  
Sbjct: 200 YAQMLEKAGCQLLTVHGRTKEQKGPLSGAASWEHIKAVRKAVAIPVFANGNIQCLQDVER 259

Query: 339 CLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKY 398
           CL++TGV+GV+SAE  L NPALF G   A W                +L  +YL +  ++
Sbjct: 260 CLRDTGVQGVMSAEGNLHNPALFEGRSPAVW----------------ELAEEYLDIVREH 291

Query: 399 PVPWRMIRSHVHKLLGDWFKIHPHIRED 425
           P P   +R+H+ KL     ++H  +RE+
Sbjct: 320 PCPLSYVRAHLFKLWHHTLQVHQELREE 291

BLAST of Chy11G191270 vs. ExPASy Swiss-Prot
Match: Q9HGN6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dus1 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 5.0e-63
Identity = 137/342 (40.06%), Postives = 194/342 (56.73%), Query Frame = 0

Query: 90  WTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFT--TC 149
           + K+GRPK I+APMVD SELP+R+L R+ GA+  Y+PM HSR+F E+E YRN  F+  T 
Sbjct: 12  YNKIGRPKRILAPMVDQSELPWRILARRSGADLCYSPMFHSRLFGESEDYRNKVFSTRTI 71

Query: 150 QEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKILEDVTLS 209
            E+RPL +QFC NDP+++L+AA+   PYCD VD+NLG                       
Sbjct: 72  PEERPLIIQFCGNDPEIMLKAAKIAAPYCDAVDVNLG----------------------- 131

Query: 210 QTKVPEHQLSSEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRI 269
                            CPQ IA++G YG+FL +N  L+++I+ KL + L +PV+ KIRI
Sbjct: 132 -----------------CPQGIAKKGKYGSFLQENWNLIESIITKLHTELSIPVTAKIRI 191

Query: 270 FPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAV-KNAVRIPVL-AN 329
           FP+ Q T++YA+M+  AG S+LAVHGR R++K      A+W  I  + KN     VL AN
Sbjct: 192 FPDPQKTLDYAKMILKAGASILAVHGRLREQKGHFTGIADWEQIQMLRKNLPSETVLFAN 251

Query: 330 GNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLDQADL 389
           GNI H +D++ C++ TGV+GVLSAE  L NP +F               T    +D  D+
Sbjct: 252 GNILHAQDIDRCIKYTGVDGVLSAEGSLYNPRIFLP-------PSSPLMTLYPRID--DM 304

Query: 390 LVDYLKLCEKYPV-----PWRMIRSHVHKLLGDWFKIHPHIR 423
             +YL +  ++ +         I+ H+ KL+     IH  IR
Sbjct: 312 CEEYLNIIREFKLESDYSSLSAIKGHLFKLMRPLLSIHTDIR 304

BLAST of Chy11G191270 vs. ExPASy Swiss-Prot
Match: P53759 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DUS1 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.7e-58
Identity = 130/353 (36.83%), Postives = 189/353 (53.54%), Query Frame = 0

Query: 92  KLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTT---CQ 151
           K+GRP  IVAPMVD SEL +R+L R+YGA  AYTPMLH+++F  ++KYR   +++     
Sbjct: 25  KIGRPTRIVAPMVDQSELAWRILSRRYGATLAYTPMLHAKLFATSKKYREDNWSSLDGSS 84

Query: 152 EDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKILEDVTLSQ 211
            DRPL VQFCANDP+ LL AA+ VE  CD VDLNLG                        
Sbjct: 85  VDRPLVVQFCANDPEYLLAAAKLVEDKCDAVDLNLG------------------------ 144

Query: 212 TKVPEHQLSSEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIF 271
                           CPQ IA++G+YG+FLM+   L+  ++  L  NL+VPV+ KIRIF
Sbjct: 145 ----------------CPQGIAKKGHYGSFLMEEWDLIHNLINTLHKNLKVPVTAKIRIF 204

Query: 272 PNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAV--RIPVLANG 331
            + + ++NYA+M+ DAG   L VHGR R++K  K   ANW  I  +++ +       ANG
Sbjct: 205 DDCEKSLNYAKMVLDAGAQFLTVHGRVREQKGQKTGLANWETIKYLRDNLPKETVFFANG 264

Query: 332 NIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLDQADLL 391
           NI + ED++ C++  G + V+SAE  L NP +F   +T           ++    + D +
Sbjct: 265 NILYPEDISRCMEHIGADAVMSAEGNLYNPGVFNVGQT---------KNKEKIFPRVDKI 324

Query: 392 V----DYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDF-NAQSKLTFE 435
           +      +K C++       ++SH  K+L  +   H  IR       +K T+E
Sbjct: 325 IREYFQIVKECQESKASKTAMKSHFFKILRPFLPHHTDIRSTLATMNAKATWE 328

BLAST of Chy11G191270 vs. ExPASy TrEMBL
Match: A0A0A0LTG2 (Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.6e-237
Identity = 417/466 (89.48%), Postives = 419/466 (89.91%), Query Frame = 0

Query: 1   MRSLSNSHLHLKSIFSLPNSLMASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTN 60
           MRSLSNSHLHLK IFSLPNSLMASSL QTLKPDL PQDLDDLLCSPS KQPQ ESSSLTN
Sbjct: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSHKQPQHESSSLTN 60

Query: 61  SNSNDSTPLLGSPTGYLSRESRIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
           S SNDSTPLLGSPTGYLSRESRIERAW HWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLM 240
           LNLG                                        CPQRIARRGNYGAFLM
Sbjct: 181 LNLG----------------------------------------CPQRIARRGNYGAFLM 240

Query: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300
           DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD
Sbjct: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300

Query: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360
           GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVN+CLQETGVEGVLSAETLLENPALFA
Sbjct: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFA 360

Query: 361 GFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420
           GFRTAEWIEGH+ENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH
Sbjct: 361 GFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420

Query: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 467
           IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA
Sbjct: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 426

BLAST of Chy11G191270 vs. ExPASy TrEMBL
Match: A0A5A7VJ66 (tRNA-dihydrouridine(16/17) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G00480 PE=4 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 6.0e-237
Identity = 422/489 (86.30%), Postives = 425/489 (86.91%), Query Frame = 0

Query: 22  MASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTNSNSNDSTPLLGSPTGYLSRES 81
           MASS TQTLKPDLPPQDLDDLLCS  QKQ Q ESSSLT SNSNDS  LLGSPTGYLS ES
Sbjct: 1   MASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTGSNSNDSKLLLGSPTGYLSGES 60

Query: 82  RIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRN 141
           RIERAW HWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA+AAYTPMLHSRIFTENEKYRN
Sbjct: 61  RIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGADAAYTPMLHSRIFTENEKYRN 120

Query: 142 MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKIL 201
           MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG   +LD EELPSIVKIL
Sbjct: 121 MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG---ELDTEELPSIVKIL 180

Query: 202 EDVTLSQT--------------------------------------------KVPEHQLS 261
           EDVTLSQT                                            KVPEH LS
Sbjct: 181 EDVTLSQTKHCPYHLASWWGHGLIELNSTVSSLASHSAAMLVGGFFLYVFLSKVPEHLLS 240

Query: 262 SEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINY 321
           S VI CRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINY
Sbjct: 241 SRVISCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINY 300

Query: 322 ARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNC 381
           ARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNC
Sbjct: 301 ARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNC 360

Query: 382 LQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYP 441
           LQETGVEGVLSAETLLENPALFAGFRTAEWIEG +ENTRDGNLDQADLLVDYLKLCEKYP
Sbjct: 361 LQETGVEGVLSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYP 420

Query: 442 VPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDS 467
           VPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDS
Sbjct: 421 VPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDS 480

BLAST of Chy11G191270 vs. ExPASy TrEMBL
Match: A0A1S3CHU6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=LOC103501114 PE=4 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 4.2e-230
Identity = 407/466 (87.34%), Postives = 411/466 (88.20%), Query Frame = 0

Query: 1   MRSLSNSHLHLKSIFSLPNSLMASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTN 60
           MRSLSNSHL+LK+IFSL NS MASS TQTLKPDLPPQDLDDLLCS  QKQ Q ESSSLT 
Sbjct: 1   MRSLSNSHLYLKTIFSLRNSFMASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTG 60

Query: 61  SNSNDSTPLLGSPTGYLSRESRIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
           SNSNDS  LLGSPTGYLS ESRIERAW HWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  SNSNDSKLLLGSPTGYLSGESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           +AAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 DAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLM 240
           LNLG                                        CPQRIARRGNYGAFLM
Sbjct: 181 LNLG----------------------------------------CPQRIARRGNYGAFLM 240

Query: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300
           DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD
Sbjct: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300

Query: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360
           GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA
Sbjct: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360

Query: 361 GFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420
           GFRTAEWIEG +ENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH
Sbjct: 361 GFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420

Query: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 467
           IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSH  AISSNA
Sbjct: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVPAISSNA 426

BLAST of Chy11G191270 vs. ExPASy TrEMBL
Match: A0A6J1FWR0 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=3662 GN=LOC111448913 PE=4 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 1.0e-212
Identity = 384/464 (82.76%), Postives = 396/464 (85.34%), Query Frame = 0

Query: 1   MRSLSNSHLHLKSIFSLPNSLMASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTN 60
           MRS SNSHLHLKSI SL  S MASS TQT   D  PQDLDDLLCS    QPQ E SSLT 
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQT---DF-PQDLDDLLCSDPHTQPQDECSSLT- 60

Query: 61  SNSNDSTPLLGSPTGYLSRESRIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             SNDST LLGSP+GYLS ESRIERAW HW+KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  -KSNDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLM 240
           LNLG                                        CPQRIARRGNYGAFLM
Sbjct: 181 LNLG----------------------------------------CPQRIARRGNYGAFLM 240

Query: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300
           DNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD
Sbjct: 241 DNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300

Query: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360
           GKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV+NCLQETGVEGVLSAETLLENPALFA
Sbjct: 301 GKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFA 360

Query: 361 GFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420
           GFRTA+W  G +E++RDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH
Sbjct: 361 GFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 418

Query: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISS 465
           IREDFNAQSKLTFEFLYNMVE+LRELQVK+PLYIKDS A AISS
Sbjct: 421 IREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPASAISS 418

BLAST of Chy11G191270 vs. ExPASy TrEMBL
Match: A0A6J1JGU3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661 GN=LOC111484251 PE=4 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.3e-211
Identity = 382/464 (82.33%), Postives = 393/464 (84.70%), Query Frame = 0

Query: 1   MRSLSNSHLHLKSIFSLPNSLMASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTN 60
           MRS SNSHLHLKSI SL  S MASS  QT   D  PQDLDDLLCS    Q Q E SSLT 
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSCAQT---DF-PQDLDDLLCSDPHTQRQDECSSLT- 60

Query: 61  SNSNDSTPLLGSPTGYLSRESRIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             SNDST LLGSP+GYL+ ESRIERAW HW+KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  -KSNDSTLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLM 240
           LNLG                                        CPQRIARRGNYGAFLM
Sbjct: 181 LNLG----------------------------------------CPQRIARRGNYGAFLM 240

Query: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300
           DNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD
Sbjct: 241 DNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300

Query: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360
           GKKFRANWSAI AVKNA+RIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA
Sbjct: 301 GKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360

Query: 361 GFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420
           GFRTA+W  G +E++RDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH
Sbjct: 361 GFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 418

Query: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISS 465
           IREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDS A AISS
Sbjct: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISS 418

BLAST of Chy11G191270 vs. NCBI nr
Match: XP_011650036.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis sativus])

HSP 1 Score: 826 bits (2134), Expect = 6.94e-301
Identity = 417/466 (89.48%), Postives = 419/466 (89.91%), Query Frame = 0

Query: 1   MRSLSNSHLHLKSIFSLPNSLMASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTN 60
           MRSLSNSHLHLK IFSLPNSLMASSL QTLKPDL PQDLDDLLCSPS KQPQ ESSSLTN
Sbjct: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSHKQPQHESSSLTN 60

Query: 61  SNSNDSTPLLGSPTGYLSRESRIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
           S SNDSTPLLGSPTGYLSRESRIERAW HWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLM 240
           LNLG                                        CPQRIARRGNYGAFLM
Sbjct: 181 LNLG----------------------------------------CPQRIARRGNYGAFLM 240

Query: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300
           DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD
Sbjct: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300

Query: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360
           GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVN+CLQETGVEGVLSAETLLENPALFA
Sbjct: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFA 360

Query: 361 GFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420
           GFRTAEWIEGH+ENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH
Sbjct: 361 GFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420

Query: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 466
           IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA
Sbjct: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 426

BLAST of Chy11G191270 vs. NCBI nr
Match: KAA0066396.1 (tRNA-dihydrouridine(16/17) synthase [Cucumis melo var. makuwa] >TYK00889.1 tRNA-dihydrouridine(16/17) synthase [Cucumis melo var. makuwa])

HSP 1 Score: 825 bits (2130), Expect = 2.72e-299
Identity = 422/489 (86.30%), Postives = 425/489 (86.91%), Query Frame = 0

Query: 22  MASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTNSNSNDSTPLLGSPTGYLSRES 81
           MASS TQTLKPDLPPQDLDDLLCS  QKQ Q ESSSLT SNSNDS  LLGSPTGYLS ES
Sbjct: 1   MASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTGSNSNDSKLLLGSPTGYLSGES 60

Query: 82  RIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRN 141
           RIERAW HWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA+AAYTPMLHSRIFTENEKYRN
Sbjct: 61  RIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGADAAYTPMLHSRIFTENEKYRN 120

Query: 142 MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKIL 201
           MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG   +LD EELPSIVKIL
Sbjct: 121 MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG---ELDTEELPSIVKIL 180

Query: 202 EDVTLSQTK--------------------------------------------VPEHQLS 261
           EDVTLSQTK                                            VPEH LS
Sbjct: 181 EDVTLSQTKHCPYHLASWWGHGLIELNSTVSSLASHSAAMLVGGFFLYVFLSKVPEHLLS 240

Query: 262 SEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINY 321
           S VI CRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINY
Sbjct: 241 SRVISCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINY 300

Query: 322 ARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNC 381
           ARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNC
Sbjct: 301 ARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNC 360

Query: 382 LQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYP 441
           LQETGVEGVLSAETLLENPALFAGFRTAEWIEG +ENTRDGNLDQADLLVDYLKLCEKYP
Sbjct: 361 LQETGVEGVLSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYP 420

Query: 442 VPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDS 466
           VPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDS
Sbjct: 421 VPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDS 480

BLAST of Chy11G191270 vs. NCBI nr
Match: XP_008462837.1 (PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis melo])

HSP 1 Score: 801 bits (2070), Expect = 3.92e-291
Identity = 407/466 (87.34%), Postives = 411/466 (88.20%), Query Frame = 0

Query: 1   MRSLSNSHLHLKSIFSLPNSLMASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTN 60
           MRSLSNSHL+LK+IFSL NS MASS TQTLKPDLPPQDLDDLLCS  QKQ Q ESSSLT 
Sbjct: 1   MRSLSNSHLYLKTIFSLRNSFMASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTG 60

Query: 61  SNSNDSTPLLGSPTGYLSRESRIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
           SNSNDS  LLGSPTGYLS ESRIERAW HWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  SNSNDSKLLLGSPTGYLSGESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           +AAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 DAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLM 240
           LNLG                                        CPQRIARRGNYGAFLM
Sbjct: 181 LNLG----------------------------------------CPQRIARRGNYGAFLM 240

Query: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300
           DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD
Sbjct: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300

Query: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360
           GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA
Sbjct: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360

Query: 361 GFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420
           GFRTAEWIEG +ENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH
Sbjct: 361 GFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420

Query: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 466
           IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSH  AISSNA
Sbjct: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVPAISSNA 426

BLAST of Chy11G191270 vs. NCBI nr
Match: KAE8652323.1 (hypothetical protein Csa_021950 [Cucumis sativus])

HSP 1 Score: 791 bits (2042), Expect = 3.26e-287
Identity = 397/445 (89.21%), Postives = 399/445 (89.66%), Query Frame = 0

Query: 22  MASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTNSNSNDSTPLLGSPTGYLSRES 81
           MASSL QTLKPDL PQDLDDLLCSPS KQPQ ESSSLTNS SNDSTPLLGSPTGYLSRES
Sbjct: 1   MASSLIQTLKPDLAPQDLDDLLCSPSHKQPQHESSSLTNSTSNDSTPLLGSPTGYLSRES 60

Query: 82  RIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRN 141
           RIERAW HWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRN
Sbjct: 61  RIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRN 120

Query: 142 MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKIL 201
           MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG                 
Sbjct: 121 MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLG----------------- 180

Query: 202 EDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPV 261
                                  CPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPV
Sbjct: 181 -----------------------CPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPV 240

Query: 262 SCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIP 321
           SCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIP
Sbjct: 241 SCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIP 300

Query: 322 VLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLD 381
           VLANGNIRHMEDVN+CLQETGVEGVLSAETLLENPALFAGFRTAEWIEGH+ENTRDGNLD
Sbjct: 301 VLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLD 360

Query: 382 QADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE 441
           QADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE
Sbjct: 361 QADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE 405

Query: 442 RLRELQVKMPLYIKDSHALAISSNA 466
           RLRELQVKMPLYIKDSHALAISSNA
Sbjct: 421 RLRELQVKMPLYIKDSHALAISSNA 405

BLAST of Chy11G191270 vs. NCBI nr
Match: XP_038901742.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida])

HSP 1 Score: 763 bits (1971), Expect = 4.21e-276
Identity = 391/466 (83.91%), Postives = 399/466 (85.62%), Query Frame = 0

Query: 1   MRSLSNSHLHLKSIFSLPNSLMASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTN 60
           MRS  NSHLH K+IFS  +SLMASS TQTLKPDLP QDLDD LCS  Q Q QQE SS T 
Sbjct: 1   MRSPYNSHLHFKTIFSFRSSLMASSFTQTLKPDLP-QDLDDRLCSDPQNQQQQECSSFTK 60

Query: 61  SNSNDSTPLLGSPTGYLSRESRIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
           SN  DST LL SP+GYL  ESRIERAW HW KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  SN--DSTLLLASPSGYLCGESRIERAWAHWRKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLM 240
           LNLG                                        CPQRIARRGNYGAFLM
Sbjct: 181 LNLG----------------------------------------CPQRIARRGNYGAFLM 240

Query: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300
           DNLPLVKAIVEKLASNLQVPVSCKIRIF NLQDTINYARMLEDAGCSLLAVHGRTRDEKD
Sbjct: 241 DNLPLVKAIVEKLASNLQVPVSCKIRIFSNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 300

Query: 301 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 360
           GKKFRANWSAISAVKNAVR+PVLANGNIRHMEDV NCLQETGVEGVLSAETLLENPALFA
Sbjct: 301 GKKFRANWSAISAVKNAVRVPVLANGNIRHMEDVYNCLQETGVEGVLSAETLLENPALFA 360

Query: 361 GFRTAEWIEGHKENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420
           GFRTAEWI+GH+ENTRDGNLDQADLLVDYLK CEKYPVPWRMIRSHVHKLLGDWFKIHPH
Sbjct: 361 GFRTAEWIKGHEENTRDGNLDQADLLVDYLKFCEKYPVPWRMIRSHVHKLLGDWFKIHPH 420

Query: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 466
           IREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDSHA AI+SNA
Sbjct: 421 IREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIASNA 423

BLAST of Chy11G191270 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 585.1 bits (1507), Expect = 5.0e-167
Identity = 301/455 (66.15%), Postives = 346/455 (76.04%), Query Frame = 0

Query: 3   SLSNSHLHLKSIFSLPNSLMASSLTQTLKPDLPPQDLDDLLCSPSQKQPQQESSSLTNSN 62
           +LSN    L++  SL +   A +     KPD P Q LDD+LCS  Q+  Q E +      
Sbjct: 4   NLSNLRF-LRTRKSLISQTRAMTQNPDPKPD-PSQVLDDILCS-EQRDGQIEETV----- 63

Query: 63  SNDSTPL-LGSPTGYLSRESRIERAWVHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAE 122
             D+ P  LGSP+  LS ++R+ERAW HW KLGRPK IVAPMVDNSELPFRLLC+KYGA+
Sbjct: 64  --DTAPASLGSPSRVLSIDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQ 123

Query: 123 AAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDL 182
           AAYTPMLHSRIFTE EKYRN EFTTC+EDRPLFVQFCANDPD LLEAA+RVEPYCDYVD+
Sbjct: 124 AAYTPMLHSRIFTETEKYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDI 183

Query: 183 NLGYPRDLDIEELPSIVKILEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLMD 242
           NLG                                        CPQRIARRGNYGAFLMD
Sbjct: 184 NLG----------------------------------------CPQRIARRGNYGAFLMD 243

Query: 243 NLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDG 302
           NLPLVK++VEKLA NL VPVSCKIRIFPNL+DT+ YA+MLEDAGCSLLAVHGRTRDEKDG
Sbjct: 244 NLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRTRDEKDG 303

Query: 303 KKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAG 362
           KKFRA+WSAI  VKNA+RIPVLANGN+R +EDV+NC++ETGVEGVLSAETLLENPA FAG
Sbjct: 304 KKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETLLENPAAFAG 363

Query: 363 FRTAEWI-EGHKENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 422
           FRTAEW  +  +E   DG LDQ DL+V+YLKLCEK+PVPWRMIRSHVHK+LG+WF+IHP 
Sbjct: 364 FRTAEWAKDNEEEGFVDGGLDQGDLVVEYLKLCEKHPVPWRMIRSHVHKMLGEWFRIHPQ 408

Query: 423 IREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIK 456
           +RE  NAQ+ LTFEFLY +V++LREL  ++PLY K
Sbjct: 424 VREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKK 408

BLAST of Chy11G191270 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.6e-24
Identity = 83/305 (27.21%), Postives = 129/305 (42.30%), Query Frame = 0

Query: 97  KLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNME------------- 156
           KL++APMV    L FR+L  +YGA+  Y   +      + E+  N+              
Sbjct: 6   KLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVEKGTDN 65

Query: 157 --FTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKI 216
             F+TC E++   V Q   +D    L+A+  V      +D+N+G                
Sbjct: 66  VVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMG---------------- 125

Query: 217 LEDVTLSQTKVPEHQLSSEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVP 276
                                   CP+  + +G  GA L+    L+  I+  L  NL VP
Sbjct: 126 ------------------------CPKAFSIQGGMGAALLSKPELIHDILATLKRNLDVP 185

Query: 277 VSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRI 336
           V+CKIR+  +  DT+  AR +E  G   LAVHG  R   D  +  A W  I+ V  A+ I
Sbjct: 186 VTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIADRPRDPAKWDEIADVVAALSI 245

Query: 337 PVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNL 386
           PV+ANG++   +D +     TG   V+ A   + N ++F+    + W +  K+  R   L
Sbjct: 246 PVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIFSPKGKSHWEDVKKKYLRKSIL 268

BLAST of Chy11G191270 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.6e-19
Identity = 54/161 (33.54%), Postives = 82/161 (50.93%), Query Frame = 0

Query: 225 CPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDA 284
           CP+  + +G  GA L+    L+  I+  L  NL VPV+CKIR+  +  DT+  AR +E  
Sbjct: 71  CPKAFSIQGGMGAALLSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKL 130

Query: 285 GCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVE 344
           G   LAVHG  R   D  +  A W  I+ V  A+ IPV+ANG++   +D +     TG  
Sbjct: 131 GVPALAVHG--RKIADRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAA 190

Query: 345 GVLSAETLLENPALFAGFRTAEWIEGHKENTRDGNLDQADL 386
            V+ A   + N ++F+    + W +  K+  R   L   D+
Sbjct: 191 SVMVARGAMWNASIFSPKGKSHWEDVKKKYLRKSILWNNDV 229

BLAST of Chy11G191270 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 3.9e-10
Identity = 54/247 (21.86%), Postives = 97/247 (39.27%), Query Frame = 0

Query: 95  RPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLF 154
           R KL +AP+     LPFR LC+  GA+     M       + +            +    
Sbjct: 337 RDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFG 396

Query: 155 VQFCANDPDVLLEAARRVEPYC--DYVDLNLGYPRDLDIEELPSIVKILEDVTLSQTKVP 214
           VQ C + PD +      ++  C  D++D+N+G P D+ + +                   
Sbjct: 397 VQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNK------------------- 456

Query: 215 EHQLSSEVILCRCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIR--IFPN 274
                                + G+ L++    +K IVE  +S ++ P++ K+R   F  
Sbjct: 457 ---------------------SAGSALLNKPLRMKNIVEVSSSIVETPITIKVRTAFFEG 516

Query: 275 LQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAI-SAVKNA-VRIPVLANGNI 334
                +    + + G + + +HGR+R ++  K   A+W  I    KNA   + V+ NG++
Sbjct: 517 KNRIDSLIADIGNWGATAVTIHGRSRQQRYSKS--ADWDYIYQCTKNATTNLQVIGNGDV 541

Query: 335 RHMEDVN 336
               D N
Sbjct: 577 YSYLDWN 541

BLAST of Chy11G191270 vs. TAIR 10
Match: AT3G63510.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 3.7e-05
Identity = 67/288 (23.26%), Postives = 98/288 (34.03%), Query Frame = 0

Query: 96  PKLIVAPMVDNSELPFRLLCRKYGAEA-AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLF 155
           P   VAPM+D ++  +R L R     A  YT M+ +      +   +       +  P+ 
Sbjct: 52  PSFSVAPMMDWTDNHYRTLARLITKHAWLYTEMIAAETLVHQQTNLDRFLAFSPQQHPIV 111

Query: 156 VQFCANDPDVLLEAARRVEPYCDYVDLNLGYPRDLDIEELPSIVKILEDVTLSQTKVPEH 215
           +Q   ++ + L +AA+  + Y  Y ++NL                               
Sbjct: 112 LQLGGSNVENLAKAAKLSDAY-GYDEINLN------------------------------ 171

Query: 216 QLSSEVILCRCPQ-RIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRI------ 275
                   C CP  ++A  G +G  LM    LV   +  +A+N  VPV+ K RI      
Sbjct: 172 --------CGCPSPKVAGHGCFGVSLMLKPKLVGEAMSAIAANTNVPVTVKCRIGVDNHD 231

Query: 276 -FPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVL--- 335
            +  L D I     L         VH R          +A    IS   N  RIP L   
Sbjct: 232 SYDELCDFIYKVSTLSPT--RHFIVHSR----------KALLGGISPADNR-RIPPLKYE 286

Query: 336 ---------------ANGNIRHMEDVNNCLQETGVEGVLSAETLLENP 357
                           NG I  +  VN  L+E G  GV+       NP
Sbjct: 292 YYYALVRDFPDLRFTINGGITSVSKVNAALKE-GAHGVMVGRAAYNNP 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8K5829.1e-7342.01tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=101... [more]
Q8C2P31.5e-7241.72tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN... [more]
Q6P1R45.9e-7242.38tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=... [more]
Q9HGN65.0e-6340.06tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (st... [more]
P537591.7e-5836.83tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (str... [more]
Match NameE-valueIdentityDescription
A0A0A0LTG21.6e-23789.48Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV... [more]
A0A5A7VJ666.0e-23786.30tRNA-dihydrouridine(16/17) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3CHU64.2e-23087.34tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1FWR01.0e-21282.76tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=36... [more]
A0A6J1JGU34.3e-21182.33tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
XP_011650036.16.94e-30189.48tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis sativus][more]
KAA0066396.12.72e-29986.30tRNA-dihydrouridine(16/17) synthase [Cucumis melo var. makuwa] >TYK00889.1 tRNA-... [more]
XP_008462837.13.92e-29187.34PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis melo][more]
KAE8652323.13.26e-28789.21hypothetical protein Csa_021950 [Cucumis sativus][more]
XP_038901742.14.21e-27683.91tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G67220.15.0e-16766.15FMN-linked oxidoreductases superfamily protein [more]
AT3G49640.11.6e-2427.21Aldolase-type TIM barrel family protein [more]
AT3G49640.21.6e-1933.54Aldolase-type TIM barrel family protein [more]
AT4G38890.13.9e-1021.86FMN-linked oxidoreductases superfamily protein [more]
AT3G63510.13.7e-0523.26FMN-linked oxidoreductases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035587DUS-like, FMN-binding domainPFAMPF01207Duscoord: 99..188
e-value: 6.9E-22
score: 77.9
coord: 224..430
e-value: 6.7E-44
score: 150.2
IPR035587DUS-like, FMN-binding domainCDDcd02801DUS_like_FMNcoord: 97..367
e-value: 1.16493E-98
score: 294.017
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 221..368
e-value: 4.9E-44
score: 152.4
coord: 95..193
e-value: 1.4E-27
score: 98.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..74
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 29..75
NoneNo IPR availablePANTHERPTHR11082:SF35BNAA09G07510D PROTEINcoord: 77..188
coord: 223..455
NoneNo IPR availablePANTHERPTHR11082TRNA-DIHYDROURIDINE SYNTHASEcoord: 77..188
coord: 223..455
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 96..412

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G191270.1Chy11G191270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002943 tRNA dihydrouridine synthesis
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0017150 tRNA dihydrouridine synthase activity
molecular_function GO:0003824 catalytic activity