Chy11G188630 (gene) Cucumber (hystrix) v1

Overview
NameChy11G188630
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAmidase domain-containing protein
LocationchrH11: 2603104 .. 2605239 (-)
RNA-Seq ExpressionChy11G188630
SyntenyChy11G188630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGACCAAAGCTTTCCCATTTACATATCCATGCTTCTGGGTTTGTGGGCAATTTTATCATCATATGGGAGTTGTAGCTTTGACACCAACTTTTCCATTGAAGAAGCAACTTTGAAAGACCTCCAATTTGCTTTCTACCAAAACAAACTCACCTCAACGCAACTTGTTGAGTTTTACCTCGAACAAGTGCGTAGATTTAACCCCATCCTGAATGGGATCATAGAAGTGAATCCAGATGCTTTAAACCAAGCCTCTCAAGCCGACCTTGAGCGCAAGAGAAGCTCACCGAGATCTTTGTCTCCACTTCATGGCATTCCTGTACTTGTAAAAGATAACATTGCAACCAAGGATAAGCTCAACACAACGGCTGGCTCTTTTGCTCTGCTTGGCTCTATTGTTCCTCGTGATGCAGGTGTGGTGACTAAGTTGAGGAAGGCAGGTGCAATTATCTTTGGGAAAGCGAGCCTGAGTGAGTGGTCCGATTTCAGGTCCTATGAACCCCCCAACGGTTGGAGCGCTAGGGGTGGCCAAGGGAAGGTGTGCAACTTTTCCAAATTCTTCAATAATTGTCAGCTTCTAAGTGAAATCACACAATAATGAATCTGTAGCACACAGTAGGACGTTACATGTCAAAATATATTTTAAATAATAGGATGATAAAGAAGATTTTGAAAGTGTAGAACTACTCAGATTCTGCCAATAAACAATCCTTTTAAGTTTTTAACTAAAAATATCTTGTTAATATCAGCCTCTAAGCTATCTTTTTTTTAAAAATCAATTAATTAGAGATTTCAATTAAAAGACAGCAAAAATTAGATTTAAATCCACAACAAACTAAGAGCAGTGCATATGTTTGCAGAATCCTTACACAATGGGAGAACCGTGTGGCTCAAGTAGCGGCTCTGCCATATCCGTTGCAGCAAATATGGTTACAGTTTCACTGGGAACCGAAACTGATGGATCAATATTGTGCCCTTCTACTCTCAACTCAGTAGTTGGCATCAAACCCACAGTAGGTCTCACAAGTCGAGCAGGGGTCGTTCCGATCTCTTTGAGGCAGGACACTGTAGGGTAATCAATTTGTTTCTGTTGAATTGTTGCTATTTATTTAAGTATCCAAAGGAAGTATTTTCTGCTGAATGAGTTATGATGGAACAGGCCTATTTGTAGAACAGTGGCAGATGCTGCTTATGTTCTAGATGCCATTGCAGGACCGGACAGATATGATAATTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGCTATGGTCAATTTCTAAGGGAAGATGGGCTGAAAGGAAAGAGAATAGGAATCGTGAGGAAATTGTATGATTTTGGCGATGATGATGTGTTCTACATTGGAGCTTTTGAGAAAGCTTTCAAAACCATGAAGTAAGAAATCAAACTCACTTTTCATAAACCATTTCTTAATAACTCATGTTAGAGGTTTTTAACCAACTCTTTTGTCTTCAATAACATGAAAGGCAAGGAGGAGCAATATTGGTAGACAATTTGACGATCAACAGCTTTCATGTAATCACTGGCAGTTCAAGTGGAGAGATGACTGCATTGCTTGCTGAGTTCAAAATATCCCTAAATGCATACCTCAAACAGCTAGTTGCATCTCCAATTCGATCCCTGTCAGATGCAATAGAATTCAACAAAAAAAACTCAAAACTTGTGAGCAATTATCATCTATTCTGTTCTAATTTTACAATCAGCATAGCAAAAAGCGGACTTTCATAACATTGAAATCTTGTCAATATGTAGGAAAAGCTAAGGGAGTATGGTCAGGAGTTATTTCTAGAAGCAGAAGCAACAAAAGGAATCGGACGTGCAGAAAAGGCATTAGCCAGATTAGCAAAACTGTCAAAAGAGGGATTTGAGAGATTGATGATTAAGAATAAGCTTGATGCAATAGCAGCTCCTGGTCGGTTAATCTCTCCTTTTCTTGCAATTGGAGGGTTTCCTGGAGTCAGTGTACCAGCTGGATATAACCCTCAAGGGCTTCCCTTTGGCATTAGCTTTGGAGGATTAAAAGGGTTCGAGCCAAGGTTGATAGAGATTGCAAATGGATTTGAGCATTTGACTATGGGTAGAAAGTCCCCTTCACTTGGGAGACATTAA

mRNA sequence

ATGGCTGACCAAAGCTTTCCCATTTACATATCCATGCTTCTGGGTTTGTGGGCAATTTTATCATCATATGGGAGTTGTAGCTTTGACACCAACTTTTCCATTGAAGAAGCAACTTTGAAAGACCTCCAATTTGCTTTCTACCAAAACAAACTCACCTCAACGCAACTTGTTGAGTTTTACCTCGAACAAGTGCGTAGATTTAACCCCATCCTGAATGGGATCATAGAAGTGAATCCAGATGCTTTAAACCAAGCCTCTCAAGCCGACCTTGAGCGCAAGAGAAGCTCACCGAGATCTTTGTCTCCACTTCATGGCATTCCTGTACTTGTAAAAGATAACATTGCAACCAAGGATAAGCTCAACACAACGGCTGGCTCTTTTGCTCTGCTTGGCTCTATTGTTCCTCGTGATGCAGGTGTGGTGACTAAGTTGAGGAAGGCAGGTGCAATTATCTTTGGGAAAGCGAGCCTGAGTGAGTGGTCCGATTTCAGGTCCTATGAACCCCCCAACGGTTGGAGCGCTAGGGGTGGCCAAGGGAAGAATCCTTACACAATGGGAGAACCGTGTGGCTCAAGTAGCGGCTCTGCCATATCCGTTGCAGCAAATATGGTTACAGTTTCACTGGGAACCGAAACTGATGGATCAATATTGTGCCCTTCTACTCTCAACTCAGTAGTTGGCATCAAACCCACAGTAGGTCTCACAAGTCGAGCAGGGGTCGTTCCGATCTCTTTGAGGCAGGACACTGTAGGGCCTATTTGTAGAACAGTGGCAGATGCTGCTTATGTTCTAGATGCCATTGCAGGACCGGACAGATATGATAATTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGCTATGGTCAATTTCTAAGGGAAGATGGGCTGAAAGGAAAGAGAATAGGAATCGTGAGGAAATTGTATGATTTTGGCGATGATGATGTGTTCTACATTGGAGCTTTTGAGAAAGCTTTCAAAACCATGAAGCAAGGAGGAGCAATATTGGTAGACAATTTGACGATCAACAGCTTTCATGTAATCACTGGCAGTTCAAGTGGAGAGATGACTGCATTGCTTGCTGAGTTCAAAATATCCCTAAATGCATACCTCAAACAGCTAGTTGCATCTCCAATTCGATCCCTGTCAGATGCAATAGAATTCAACAAAAAAAACTCAAAACTTGAAAAGCTAAGGGAGTATGGTCAGGAGTTATTTCTAGAAGCAGAAGCAACAAAAGGAATCGGACGTGCAGAAAAGGCATTAGCCAGATTAGCAAAACTGTCAAAAGAGGGATTTGAGAGATTGATGATTAAGAATAAGCTTGATGCAATAGCAGCTCCTGGTCGGTTAATCTCTCCTTTTCTTGCAATTGGAGGGTTTCCTGGAGTCAGTGTACCAGCTGGATATAACCCTCAAGGGCTTCCCTTTGGCATTAGCTTTGGAGGATTAAAAGGGTTCGAGCCAAGGTTGATAGAGATTGCAAATGGATTTGAGCATTTGACTATGGGTAGAAAGTCCCCTTCACTTGGGAGACATTAA

Coding sequence (CDS)

ATGGCTGACCAAAGCTTTCCCATTTACATATCCATGCTTCTGGGTTTGTGGGCAATTTTATCATCATATGGGAGTTGTAGCTTTGACACCAACTTTTCCATTGAAGAAGCAACTTTGAAAGACCTCCAATTTGCTTTCTACCAAAACAAACTCACCTCAACGCAACTTGTTGAGTTTTACCTCGAACAAGTGCGTAGATTTAACCCCATCCTGAATGGGATCATAGAAGTGAATCCAGATGCTTTAAACCAAGCCTCTCAAGCCGACCTTGAGCGCAAGAGAAGCTCACCGAGATCTTTGTCTCCACTTCATGGCATTCCTGTACTTGTAAAAGATAACATTGCAACCAAGGATAAGCTCAACACAACGGCTGGCTCTTTTGCTCTGCTTGGCTCTATTGTTCCTCGTGATGCAGGTGTGGTGACTAAGTTGAGGAAGGCAGGTGCAATTATCTTTGGGAAAGCGAGCCTGAGTGAGTGGTCCGATTTCAGGTCCTATGAACCCCCCAACGGTTGGAGCGCTAGGGGTGGCCAAGGGAAGAATCCTTACACAATGGGAGAACCGTGTGGCTCAAGTAGCGGCTCTGCCATATCCGTTGCAGCAAATATGGTTACAGTTTCACTGGGAACCGAAACTGATGGATCAATATTGTGCCCTTCTACTCTCAACTCAGTAGTTGGCATCAAACCCACAGTAGGTCTCACAAGTCGAGCAGGGGTCGTTCCGATCTCTTTGAGGCAGGACACTGTAGGGCCTATTTGTAGAACAGTGGCAGATGCTGCTTATGTTCTAGATGCCATTGCAGGACCGGACAGATATGATAATTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGCTATGGTCAATTTCTAAGGGAAGATGGGCTGAAAGGAAAGAGAATAGGAATCGTGAGGAAATTGTATGATTTTGGCGATGATGATGTGTTCTACATTGGAGCTTTTGAGAAAGCTTTCAAAACCATGAAGCAAGGAGGAGCAATATTGGTAGACAATTTGACGATCAACAGCTTTCATGTAATCACTGGCAGTTCAAGTGGAGAGATGACTGCATTGCTTGCTGAGTTCAAAATATCCCTAAATGCATACCTCAAACAGCTAGTTGCATCTCCAATTCGATCCCTGTCAGATGCAATAGAATTCAACAAAAAAAACTCAAAACTTGAAAAGCTAAGGGAGTATGGTCAGGAGTTATTTCTAGAAGCAGAAGCAACAAAAGGAATCGGACGTGCAGAAAAGGCATTAGCCAGATTAGCAAAACTGTCAAAAGAGGGATTTGAGAGATTGATGATTAAGAATAAGCTTGATGCAATAGCAGCTCCTGGTCGGTTAATCTCTCCTTTTCTTGCAATTGGAGGGTTTCCTGGAGTCAGTGTACCAGCTGGATATAACCCTCAAGGGCTTCCCTTTGGCATTAGCTTTGGAGGATTAAAAGGGTTCGAGCCAAGGTTGATAGAGATTGCAAATGGATTTGAGCATTTGACTATGGGTAGAAAGTCCCCTTCACTTGGGAGACATTAA

Protein sequence

MADQSFPIYISMLLGLWAILSSYGSCSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVEFYLEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGLKGKRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAEKALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGISFGGLKGFEPRLIEIANGFEHLTMGRKSPSLGRH*
Homology
BLAST of Chy11G188630 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.9e-156
Identity = 299/510 (58.63%), Postives = 385/510 (75.49%), Query Frame = 0

Query: 6   FPIYISMLLGLWAILSSYGSCS---FDTNFSIEEATLKDLQFAFYQNKLTSTQLVEFYLE 65
           F   + +L+   A++ S GS S     + FSI+EAT++D++ AF + +LTS QLVE YLE
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 66  QVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDKLNT 125
            + + NPIL+ +IE NPDAL QA  AD ER   +   L  LHG+PVL+KD+I+TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 126 TAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNP 185
           TAGSFALLGS+V RDAGVV +LR++GA+I GKASLSEW+ FRS+  P+GWSARG QGKNP
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNP 188

Query: 186 YTM-GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVV 245
           Y +   P GSSSGSAISV AN+V VSLGTETDGSIL P++ NSVVGIKP+VGLTSRAGVV
Sbjct: 189 YVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVV 248

Query: 246 PISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGLKGK 305
           PISLRQD++GPICRTV+DA ++LDAI G D  D +T  AS++IP+GGY QFL   GLKGK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 306 RIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVIT-GSSSGEMTAL 365
           R+GIV K          +    +   KT+++ GAI+++NLTI +  VI  G+ SGE  AL
Sbjct: 309 RLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIAL 368

Query: 366 LAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAE 425
           LAEFK+SLNAYLK+LV SP+RSL+D I +N++ ++ EK++E+GQE+FL AEAT G+G  E
Sbjct: 369 LAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKE 428

Query: 426 K-ALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFG 485
           K AL ++ +LS+ G E+L+ +NKLDAI   G  +S  LAIGG+PG++VPAGY+  G+P+G
Sbjct: 429 KTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYG 488

Query: 486 ISFGGLKGFEPRLIEIANGFEHLTMGRKSP 510
           ISFGGL+  EP+LIEIA  FE  T+ RK P
Sbjct: 489 ISFGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of Chy11G188630 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.0e-81
Identity = 202/512 (39.45%), Postives = 291/512 (56.84%), Query Frame = 0

Query: 31  NFSIEEATLKDLQFAFYQNKLTSTQLVEFYLEQVRRFNPILNGIIEVNPDALNQASQADL 90
           N ++E+AT+  LQ       LTST +V  YL++  + NP +NGI+++NPD L  AS+ D 
Sbjct: 63  NITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDD 122

Query: 91  ERKRSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAI 150
           ER     R   PLHGIP +VKDN ATKDK++TTAGS+ALLGSIVPRDA VV +LR+AGA+
Sbjct: 123 ERANGIIR--GPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAV 182

Query: 151 IFGKASLSEWSDFRSYEPPNGWSARGGQGKNPYTMG-EPCGSSSGSAISVAANMVTVSLG 210
           +FG A+LSEW+D RS +   G+SARGGQ + P+ +   P GSSSGSAISVA+NM+  +LG
Sbjct: 183 LFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALG 242

Query: 211 TETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAG 270
           TETDGSI+ P+  N VVG+KPTVGLTSR GV+P S  QDT GPI RTV DA YV  ++ G
Sbjct: 243 TETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWG 302

Query: 271 PDRYDNSTIEASKYIPK-GGYGQFL-REDGLKGKRIGIV-RKLYDFGDDDVFYIGAFEKA 330
            D  D  T+  +   P+ G Y +FL  +  L+G R G+  ++L+     D   I    + 
Sbjct: 303 IDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKAD--EIDRLLEV 362

Query: 331 FKTMKQGGAILVDNLTINSFHVITGS---------SSGEMTALLAEFKISLNAYLKQLVA 390
            K +++ GAI+ +N    +  VI+           +  E T +  +F  ++ +YL ++  
Sbjct: 363 VKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKN 422

Query: 391 SPIRSLSDAIEFNKKNSKLEKLR-------EYGQELFLEAEATKGIGRAE--KALARLAK 450
           + I SL D +E+N K    E  +         GQ+ FL +    G+      +A+  + +
Sbjct: 423 TEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRR 482

Query: 451 LSK-EGFERLM---IKNKLDAIAAPGRLISPFLAI-------GGFPGVSVPAGYNPQGLP 510
            S+ EG +  +        D+    G L+    +I        G+P +++P G    G P
Sbjct: 483 TSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGRP 542

BLAST of Chy11G188630 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.1e-71
Identity = 192/505 (38.02%), Postives = 266/505 (52.67%), Query Frame = 0

Query: 42  LQFAFYQNKLTSTQLVEFYLEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLS 101
           LQ  + Q  +    +V+ Y+ ++   N  +  + E+NPDAL  A Q D ERK    R   
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLR--G 153

Query: 102 PLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS 161
           PLHG+P+++K+NI T DK+++TAGS+A+ G+    DA V TKLR+AG +I GK+  S+W+
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 162 DFRSYEPPNGWSARGGQGKNPYTMG-EPCGSSSGSAISVAANMVTVSLGTETDGSILCPS 221
           +FRS    NGWSA GGQ    Y    +P GSSSGS ++    +   +LGTET GSI+ P+
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 222 TLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEA 281
             +++VG+KPTVGLTSR  VVPIS RQDTVGP+ R+V DAAY+L  IAG D  DN T  A
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-SA 333

Query: 282 SKYIPKGGYGQFLREDGLKGKRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDN 341
             +     Y +    + LKGKRIG+ R +          +  F +A   MK+ GAI+V+N
Sbjct: 334 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVEN 393

Query: 342 LTINSFHVITGSSSGEMTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLR 401
               SF     S   +   L A+   +L A+ KQL  +P  +++D          LE LR
Sbjct: 394 TDFTSFAEFAQSPIPD-DILYADSLTNLPAFFKQLKVNP-HNITD----------LESLR 453

Query: 402 EYGQELFLEAEATKGIGRAEKALARLAKLSKEGFERLMIKN----------------KLD 461
            + Q   LE   ++   R + AL +  K +   F  +  KN                KLD
Sbjct: 454 RFTQHHRLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLD 513

Query: 462 AIAAPGRLISPFL-AIGGFPGVSVPAGYNPQ---------------GLPFGISFGGLKGF 514
           A   P  L SP++ A+ G P ++VP G  P                G+P GI F G    
Sbjct: 514 AAVLPTDL-SPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWS 573

BLAST of Chy11G188630 vs. ExPASy Swiss-Prot
Match: Q8RC40 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=gatA PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.1e-39
Identity = 148/496 (29.84%), Postives = 249/496 (50.20%), Query Frame = 0

Query: 38  TLKDLQFAFYQNKLTSTQLVEFYLEQVRRFNPILNGIIEVNPD-ALNQASQADLERKRSS 97
           T+ +L+   ++ ++++ ++ + YLE+++   P ++ ++ +  + AL +A  AD   K   
Sbjct: 7   TVHELKELLHKREVSAQEVTKSYLERIKEVEPKIDALVTITEEFALERAKVADEMIKNGE 66

Query: 98  PRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKAS 157
            + L+   GIPV++KDNI+T + + TT  S  L   I P +A VV +L   G +I GK++
Sbjct: 67  AKDLT---GIPVIIKDNIST-EGIRTTCSSKMLENYIPPYNATVVERLLNEGVVILGKSN 126

Query: 158 LSEWSDFRSYEPPNGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGS 217
           L E++   S E     ++     KNP+ +   P GSS GSA +VAA+    +LG++T GS
Sbjct: 127 LDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAVAADEAAFALGSDTGGS 186

Query: 218 ILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDN 277
           I  P++L  VVG+KPT GL SR G+V  +   D +GP  + V D A VL+AIAG D  D+
Sbjct: 187 IRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNAIAGHDPMDS 246

Query: 278 STIEASKYIPKGGYGQFLREDGLKGKRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGA 337
           ++++    I K  Y  +L+ED +KG RIG+ ++ +  G ++       EKA K  +  GA
Sbjct: 247 TSVK----IEKPDYTSYLKED-IKGLRIGVAKEFFGAGIEEGVK-ETVEKAIKVFEDLGA 306

Query: 338 ILVD------NLTINSFHVITGSSSGEMTALLAEFKISLNAYL----KQLVASPIRSLSD 397
            ++D         + ++++I   +S E ++ LA +      ++    + LV   + S S+
Sbjct: 307 EIIDISVPYVEYALPAYYII---ASAEASSNLARYDGIRYGHIAKNYEDLVDMYMTSRSE 366

Query: 398 AIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAEKALARLAKLSKEGFERLMIKNKLDA 457
              F K+  +   L  Y       A ++       K   ++  L K  FER     K D 
Sbjct: 367 G--FGKEVKRRIMLGTY-------ALSSGYYDAYYKKALKVRTLIKNDFERAF--EKCDV 426

Query: 458 IAAP-------------GRLISPFLA--------IGGFPGVSVPAGYNPQGLPFGISFGG 501
           I  P                ++ +LA        I G P +S+P G + +GLP G+   G
Sbjct: 427 IVGPTSPTVAFKIGERTNDPLAMYLADIYTVSVNIAGLPAISIPCGLS-EGLPVGLQIIG 472

BLAST of Chy11G188630 vs. ExPASy Swiss-Prot
Match: Q3AKH0 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Synechococcus sp. (strain CC9605) OX=110662 GN=gatA PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.6e-38
Identity = 137/480 (28.54%), Postives = 232/480 (48.33%), Query Frame = 0

Query: 50  KLTSTQLVEFYLEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVL 109
           +++S +LV+ +L+++    P LN  +EV  D   +A  + ++  R++  +L PL G+P+ 
Sbjct: 19  EVSSRELVDQHLKRLESSEPSLNAFVEVTADQA-RAEASRIDEARAAGEALGPLAGLPLA 78

Query: 110 VKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPP 169
           +KDN+ TK  + TT  S  L   + P ++ V  +L +AG ++ GK +L E++   S E  
Sbjct: 79  IKDNLCTKG-VRTTCSSRMLEQFVPPYESTVTERLWQAGGVLVGKTNLDEFAMGGSTE-- 138

Query: 170 NGWSARGGQGKNPY-TMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGI 229
              ++  G  +NP+ T   P GSS GSA +VAA     SLG++T GSI  P++   VVG+
Sbjct: 139 ---TSAFGATQNPWNTAHVPGGSSGGSAAAVAAGSCVASLGSDTGGSIRQPASFCGVVGL 198

Query: 230 KPTVGLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGG 289
           KPT G  SR G+V  +   D VGP   +VAD A +L  IAGPD  D++ ++A+  +   G
Sbjct: 199 KPTYGRVSRWGLVAFASSLDQVGPFAGSVADVAELLQVIAGPDPRDSTCLDAAVPVFSDG 258

Query: 290 YGQFLREDGLKGKRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVD------NLT 349
             Q      +KG ++G++++ +D    D     + + +   ++  GA LV+      N  
Sbjct: 259 LSQ-----SIKGLKVGLIKECFDAEGLDPEVKASVQASAAQLEALGAELVEVSCPRFNDG 318

Query: 350 INSFHVITGSSSGEMTALLAEFKISLNAY-LKQLVASPIRSLSDAIEFNKKNSKLEKLRE 409
           I +++VI  S +    A     K    A   + L A   RS ++A  F  +  +   +  
Sbjct: 319 IATYYVIAPSEASANLARYDGVKYGFRAEDAESLAAMTARSRAEA--FGAEVQRRILIGT 378

Query: 410 YGQELFLEAEATKGIGRAEKALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLA--- 469
           Y       A +   +    K   ++  L +  F+       +D +  P    + F A   
Sbjct: 379 Y-------ALSAGYVDAYYKKAQQVRTLIRRDFDAAF--KSVDVLLTPTAPSTAFKAGAH 438

Query: 470 ------------------IGGFPGVSVPAGYNPQGLPFGISFGGLKGFEPRLIEIANGFE 501
                             + G P +SVP G++  GLP G+   G    EPRL+++A+ +E
Sbjct: 439 ADDPLAMYLADLLTIPVNLAGLPAISVPCGFSAAGLPIGMQLIGNVLDEPRLLQVAHQYE 475

BLAST of Chy11G188630 vs. ExPASy TrEMBL
Match: A0A0A0LLK0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.8e-274
Identity = 493/515 (95.73%), Postives = 501/515 (97.28%), Query Frame = 0

Query: 1   MADQSFPIYISMLLGLWAILSSYGSCSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVEFY 60
           MA QSFPIYISMLLGL AILSSYGSCSFDTNFSIEEATLKDLQ AFYQNKLTSTQLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPV VKDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVFVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYE PNGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGLKG 300
           VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKY+PKGGYGQFL+EDGLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMTAL 360
           KRIGIVRKLYDFG DDVFYIGAFEK FKT+KQGGAILVDNLTINSFHVITGSSSGE TA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 LAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAE 420
           LAEFKIS+N YLKQLVASPIRSLSDAIEFN+KNSKLEKL+EYGQELFLEAEATKGIG AE
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 K-ALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFG 480
           K ALARLAKLSKEGFERLMIKNKLDAIAAPGRLIS FLAIGGFPGVSVPAGYNPQGLPFG
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 ISFGGLKGFEPRLIEIANGFEHLTMGRKSPSLGRH 515
           I FGGLKGFEPRLIEIA GFEHLTMGRKSPSLGRH
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGRH 515

BLAST of Chy11G188630 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.3e-268
Identity = 489/517 (94.58%), Postives = 494/517 (95.55%), Query Frame = 0

Query: 1   MADQSFPIYISMLLGLWAILSSY--GSCSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVE 60
           MA QSFPIYISMLLGL AILS Y  GSCSFDTNFSIEEATLKD Q AFYQNKLTS QLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKD 120
           FYLEQVRR NPIL GIIEVNPDALNQASQADL+RKRSS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGL 300
           GVVPISLRQDTVGPICRTVADAAYVLDAIAG DRYDNSTIEASKYIP+GGYGQFLR +GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMT 360
           KGKRIGIVRKLYDFG DDVFYIGAFEK FKT+KQGGAILVDNLTIN F VITGSSSGE T
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGR 420
           ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEK-ALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480
           AEK ALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGISFGGLKGFEPRLIEIANGFEHLTMGRKSPSLGRH 515
           FGI FGGLKGF+PRLIEIA GFEHLTMGRKSPSLGRH
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGRH 517

BLAST of Chy11G188630 vs. ExPASy TrEMBL
Match: A0A1S3C7A8 (putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.3e-268
Identity = 489/517 (94.58%), Postives = 494/517 (95.55%), Query Frame = 0

Query: 1   MADQSFPIYISMLLGLWAILSSY--GSCSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVE 60
           MA QSFPIYISMLLGL AILS Y  GSCSFDTNFSIEEATLKD Q AFYQNKLTS QLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKD 120
           FYLEQVRR NPIL GIIEVNPDALNQASQADL+RKRSS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGL 300
           GVVPISLRQDTVGPICRTVADAAYVLDAIAG DRYDNSTIEASKYIP+GGYGQFLR +GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMT 360
           KGKRIGIVRKLYDFG DDVFYIGAFEK FKT+KQGGAILVDNLTIN F VITGSSSGE T
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGR 420
           ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEK-ALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480
           AEK ALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGISFGGLKGFEPRLIEIANGFEHLTMGRKSPSLGRH 515
           FGI FGGLKGF+PRLIEIA GFEHLTMGRKSPSLGRH
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGRH 517

BLAST of Chy11G188630 vs. ExPASy TrEMBL
Match: A0A1S3C652 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.9e-263
Identity = 485/517 (93.81%), Postives = 490/517 (94.78%), Query Frame = 0

Query: 1   MADQSFPIYISMLLGLWAILSSY--GSCSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVE 60
           MA QSFPIYISMLLGL AILS Y  GSCSFDTNFSIEEATLKD Q AFYQNKLTS QLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKD 120
           FYLEQVRR NPIL GIIEVNPDALNQASQADL+RKRSS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGL 300
           GVVPISLRQDTVG    TVADAAYVLDAIAG DRYDNSTIEASKYIP+GGYGQFLR +GL
Sbjct: 241 GVVPISLRQDTVG----TVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMT 360
           KGKRIGIVRKLYDFG DDVFYIGAFEK FKT+KQGGAILVDNLTIN F VITGSSSGE T
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGR 420
           ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEK-ALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480
           AEK ALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGISFGGLKGFEPRLIEIANGFEHLTMGRKSPSLGRH 515
           FGI FGGLKGF+PRLIEIA GFEHLTMGRKSPSLGRH
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGRH 513

BLAST of Chy11G188630 vs. ExPASy TrEMBL
Match: E5GC08 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.0e-262
Identity = 479/506 (94.66%), Postives = 484/506 (95.65%), Query Frame = 0

Query: 12  MLLGLWAILSSY--GSCSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVEFYLEQVRRFNP 71
           MLLGL AILS Y  GSCSFDTNFSIEEATLKD Q AFYQNKLTS QLVEFYLEQVRR NP
Sbjct: 1   MLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNP 60

Query: 72  ILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 131
           IL GIIEVNPDALNQASQADL+RKRSS RSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL
Sbjct: 61  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 120

Query: 132 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNPYTMGEPC 191
           LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQGKNPYTMGEPC
Sbjct: 121 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPC 180

Query: 192 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 251
           GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT
Sbjct: 181 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 240

Query: 252 VGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGLKGKRIGIVRKL 311
           VGPICRTVADAAYVLDAIAG DRYDNSTIEASKYIP+GGYGQFLR +GLKGKRIGIVRKL
Sbjct: 241 VGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKL 300

Query: 312 YDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMTALLAEFKISLN 371
           YDFG DDVFYIGAFEK FKT+KQGGAILVDNLTIN F VITGSSSGE TALLAEFKISLN
Sbjct: 301 YDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAEFKISLN 360

Query: 372 AYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAEK-ALARLAK 431
           AYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG AEK ALARLAK
Sbjct: 361 AYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARLAK 420

Query: 432 LSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGISFGGLKGF 491
           LSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGI FGGLKGF
Sbjct: 421 LSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLKGF 480

Query: 492 EPRLIEIANGFEHLTMGRKSPSLGRH 515
           +PRLIEIA GFEHLTMGRKSPSLGRH
Sbjct: 481 DPRLIEIAYGFEHLTMGRKSPSLGRH 506

BLAST of Chy11G188630 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 494/515 (95.92%), Postives = 502/515 (97.48%), Query Frame = 0

Query: 1   MADQSFPIYISMLLGLWAILSSYGSCSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVEFY 60
           MA QSFPIYISMLLGL AILSSYGSCSFDTNFSIEEATLKDLQ AFYQNKLTSTQLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYE PNGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGLKG 300
           VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKY+PKGGYGQFL+EDGLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMTAL 360
           KRIGIVRKLYDFG DDVFYIGAFEK FKT+KQGGAILVDNLTINSFHVITGSSSGE TA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 LAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAE 420
           LAEFKIS+N YLKQLVASPIRSLSDAIEFN+KNSKLEKL+EYGQELFLEAEATKGIG AE
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 KA-LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFG 480
           KA LARLAKLSKEGFERLMIKNKLDAIAAPGRLIS FLAIGGFPGVSVPAGYNPQGLPFG
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 ISFGGLKGFEPRLIEIANGFEHLTMGRKSPSLGRH 514
           I FGGLKGFEPRLIEIA GFEHLTMGRKSPSLGRH
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGRH 515

BLAST of Chy11G188630 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 931 bits (2405), Expect = 0.0
Identity = 489/517 (94.58%), Postives = 494/517 (95.55%), Query Frame = 0

Query: 1   MADQSFPIYISMLLGLWAILSSYGS--CSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVE 60
           MA QSFPIYISMLLGL AILS YGS  CSFDTNFSIEEATLKD Q AFYQNKLTS QLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKD 120
           FYLEQVRR NPIL GIIEVNPDALNQASQADL+RKRSS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGL 300
           GVVPISLRQDTVGPICRTVADAAYVLDAIAG DRYDNSTIEASKYIP+GGYGQFLR +GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMT 360
           KGKRIGIVRKLYDFG DDVFYIGAFEK FKT+KQGGAILVDNLTIN F VITGSSSGE T
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGR 420
           ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKA-LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480
           AEKA LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGISFGGLKGFEPRLIEIANGFEHLTMGRKSPSLGRH 514
           FGI FGGLKGF+PRLIEIA GFEHLTMGRKSPSLGRH
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGRH 517

BLAST of Chy11G188630 vs. NCBI nr
Match: XP_008457662.1 (PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo])

HSP 1 Score: 915 bits (2365), Expect = 0.0
Identity = 485/517 (93.81%), Postives = 490/517 (94.78%), Query Frame = 0

Query: 1   MADQSFPIYISMLLGLWAILSSYGS--CSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVE 60
           MA QSFPIYISMLLGL AILS YGS  CSFDTNFSIEEATLKD Q AFYQNKLTS QLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKD 120
           FYLEQVRR NPIL GIIEVNPDALNQASQADL+RKRSS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGL 300
           GVVPISLRQDTVG    TVADAAYVLDAIAG DRYDNSTIEASKYIP+GGYGQFLR +GL
Sbjct: 241 GVVPISLRQDTVG----TVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMT 360
           KGKRIGIVRKLYDFG DDVFYIGAFEK FKT+KQGGAILVDNLTIN F VITGSSSGE T
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGR 420
           ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKA-LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480
           AEKA LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGISFGGLKGFEPRLIEIANGFEHLTMGRKSPSLGRH 514
           FGI FGGLKGF+PRLIEIA GFEHLTMGRKSPSLGRH
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGRH 513

BLAST of Chy11G188630 vs. NCBI nr
Match: ADN34007.1 (amidase [Cucumis melo subsp. melo])

HSP 1 Score: 911 bits (2355), Expect = 0.0
Identity = 479/506 (94.66%), Postives = 484/506 (95.65%), Query Frame = 0

Query: 12  MLLGLWAILSSYGS--CSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVEFYLEQVRRFNP 71
           MLLGL AILS YGS  CSFDTNFSIEEATLKD Q AFYQNKLTS QLVEFYLEQVRR NP
Sbjct: 1   MLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNP 60

Query: 72  ILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 131
           IL GIIEVNPDALNQASQADL+RKRSS RSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL
Sbjct: 61  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 120

Query: 132 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNPYTMGEPC 191
           LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQGKNPYTMGEPC
Sbjct: 121 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPC 180

Query: 192 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 251
           GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT
Sbjct: 181 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 240

Query: 252 VGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGLKGKRIGIVRKL 311
           VGPICRTVADAAYVLDAIAG DRYDNSTIEASKYIP+GGYGQFLR +GLKGKRIGIVRKL
Sbjct: 241 VGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKL 300

Query: 312 YDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMTALLAEFKISLN 371
           YDFG DDVFYIGAFEK FKT+KQGGAILVDNLTIN F VITGSSSGE TALLAEFKISLN
Sbjct: 301 YDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAEFKISLN 360

Query: 372 AYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAEKA-LARLAK 431
           AYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIG AEKA LARLAK
Sbjct: 361 AYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARLAK 420

Query: 432 LSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGISFGGLKGF 491
           LSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGI FGGLKGF
Sbjct: 421 LSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLKGF 480

Query: 492 EPRLIEIANGFEHLTMGRKSPSLGRH 514
           +PRLIEIA GFEHLTMGRKSPSLGRH
Sbjct: 481 DPRLIEIAYGFEHLTMGRKSPSLGRH 506

BLAST of Chy11G188630 vs. NCBI nr
Match: KAE8651892.1 (hypothetical protein Csa_006820 [Cucumis sativus])

HSP 1 Score: 911 bits (2354), Expect = 0.0
Identity = 475/506 (93.87%), Postives = 484/506 (95.65%), Query Frame = 0

Query: 1   MADQSFPIYISMLLGLWAILSSYGSCSFDTNFSIEEATLKDLQFAFYQNKLTSTQLVEFY 60
           MA QSFPIYISMLLGL AILSSYGSCSFDTNFSIEEATLKDLQ AFYQNKLTSTQLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYE PNGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGLKG 300
           VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKY+PKGGYGQFL+EDGLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVITGSSSGEMTAL 360
           KRIGIVRKLYDFG DDVFYIGAFEK FKT+KQGGAILVDNLTINSFHVITGSSSGE TA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 LAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAE 420
           LAEFKIS+N YLKQLVASPIRSLSDAIEFN+KNSKLEKL+EYGQELFLEAEATKGIG AE
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 KA-LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFG 480
           KA LARLAKLSKEGFERLMIKNKLDAIAAPGRLIS FLAIGGFPGVSVPAGYNPQGLPFG
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 ISFGGLKGFEPRLIEIANGFEHLTMG 505
           I FGGLKGFEPR +        L MG
Sbjct: 481 IGFGGLKGFEPRKMTRNMEITKLAMG 506

BLAST of Chy11G188630 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 474.6 bits (1220), Expect = 1.0e-133
Identity = 266/509 (52.26%), Postives = 348/509 (68.37%), Query Frame = 0

Query: 6   FPIYISMLLGLWAILSSYGSCS---FDTNFSIEEATLKDLQFAFYQNKLTSTQLVEFYLE 65
           F   + +L+   A++ S GS S     + FSI+EAT++D++ AF + +LTS QLVE YLE
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 66  QVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDKLNT 125
            + + NPIL+ +IE NPDAL QA  AD ER   +   L  LHG+PVL+KD+I+TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 126 TAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNP 185
           TAGSFALLGS+V RDAGVV +LR++GA+I GKASLSEW+ FRS+  P+GWSA        
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA-------- 188

Query: 186 YTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVP 245
                                               PS  NSVVGIKP+VGLTSRAGVVP
Sbjct: 189 ------------------------------------PSQ-NSVVGIKPSVGLTSRAGVVP 248

Query: 246 ISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLREDGLKGKR 305
           ISLRQD++GPICRTV+DA ++LDAI G D  D +T  AS++IP+GGY QFL   GLKGKR
Sbjct: 249 ISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKR 308

Query: 306 IGIVRKLYDFGDDDVFYIGAFEKAFKTMKQGGAILVDNLTINSFHVIT-GSSSGEMTALL 365
           +GIV K          +    +   KT+++ GAI+++NLTI +  VI  G+ SGE  ALL
Sbjct: 309 LGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALL 368

Query: 366 AEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAEK 425
           AEFK+SLNAYLK+LV SP+RSL+D I +N++ ++ EK++E+GQE+FL AEAT G+G  EK
Sbjct: 369 AEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEK 428

Query: 426 -ALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGI 485
            AL ++ +LS+ G E+L+ +NKLDAI   G  +S  LAIGG+PG++VPAGY+  G+P+GI
Sbjct: 429 TALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGI 462

Query: 486 SFGGLKGFEPRLIEIANGFEHLTMGRKSP 510
           SFGGL+  EP+LIEIA  FE  T+ RK P
Sbjct: 489 SFGGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of Chy11G188630 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 135.2 bits (339), Expect = 1.5e-31
Identity = 138/518 (26.64%), Postives = 238/518 (45.95%), Query Frame = 0

Query: 19  ILSSYGSCSFDTN-FSIEEATLKDLQFAFYQNKLTSTQLVEFYLEQVRRFNPILNGIIEV 78
           I+S+  S + DT+  S  ++ +   + +    + T+ ++ + YL ++R   P L   + V
Sbjct: 25  IVSAASSKTIDTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHV 84

Query: 79  NPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRD 138
           + + L  A + D  ++ +    L PL G+ + VKDNI T+  + +TA S  L     P D
Sbjct: 85  SENVLKDAQEID--QRIAKGEELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFD 144

Query: 139 AGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNPYTMGE-PCGSSSGSA 198
           A  V K+++ G I+ GK ++ E+    + E     ++      NP+ +   P GSS GSA
Sbjct: 145 ATAVKKIKELGGIVVGKTNMDEFGMGSTTE-----ASAFQVTANPWDLSRVPGGSSGGSA 204

Query: 199 ISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRT 258
            +VAA    VSLG++T GS+  P++   VVG+KPT G  SR G++  +   D +G    T
Sbjct: 205 AAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGST 264

Query: 259 VADAAYVLDAIAGPDRYDNSTIEASKYIPKGGYGQFLRED-----GLKGKRIGIVRKLYD 318
           VADA  +L AI+G DR+D++   +SK        QFL  D      L G ++GI+R+  +
Sbjct: 265 VADAGMLLHAISGYDRFDST---SSKQDVPEFQSQFLSVDHFESKPLNGVKVGIIRETLE 324

Query: 319 FGDDDVFYIGAFEKAFKTMKQGGAILVD------NLTINSFHVITGSSSGEMTALLAEFK 378
            G D      A ++A   ++  G IL +      +L + +++VI  S S    +     +
Sbjct: 325 DGVDSGVR-SATQEAASHLEALGCILTEVSLPSFSLGLPAYYVIASSESSSNLSRYDGVR 384

Query: 379 ISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAEKALAR 438
                Y  Q++A  +  L +         +++     G              RA++    
Sbjct: 385 -----YGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTYALSAGYYDAYYKRAQQVRTL 444

Query: 439 LAKLSKEGFERLMI--------------KNKLDAIAA-PGRLISPFLAIGGFPGVSVPAG 498
           + K  K   E+  I              + K D +A   G +++  + + G P + +P G
Sbjct: 445 IRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVNLAGLPAMVLPCG 504

Query: 499 Y---NPQGLPFGISFGGLKGFEPRLIEIANGFEHLTMG 506
                P GLP G+   G    E +L+++ + FE    G
Sbjct: 505 LVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLKG 525

BLAST of Chy11G188630 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.9e-27
Identity = 84/232 (36.21%), Postives = 125/232 (53.88%), Query Frame = 0

Query: 50  KLTSTQLVEFYLEQVRRFNPILNGIIEVNPD-ALNQASQADLERKRSSPRSLSPLHGIPV 109
           ++TS +LV  YL+Q++R+N +L  ++    + A  QA +AD     S    L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQGTYLGPLHGIPY 275

Query: 110 LVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEP 169
            +KD +A      TT GS +     +  +A V  +L+ +GA++  K          S   
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTG------SMAY 335

Query: 170 PNGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVG 229
            + W   GG+ +NP+ + E   GSS+G A S +A MV  ++G+ET GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITA 395

Query: 230 IKPTVGLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIE 280
           ++PT G   R GV+ IS   D +GP CRT AD A +LDAI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 436

BLAST of Chy11G188630 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 110.5 bits (275), Expect = 4.0e-24
Identity = 82/232 (35.34%), Postives = 121/232 (52.16%), Query Frame = 0

Query: 50  KLTSTQLVEFYLEQVRRFNPILNGIIEVNPD-ALNQASQADLERKRSSPRSLSPLHGIPV 109
           ++TS +LV  YL+Q++R+N +L  ++    + A  QA +AD     S    L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQGTYLGPLHGIPY 275

Query: 110 LVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEP 169
            +KD +A      TT GS +     +  +A V  +L+ +GA++  K          S   
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTG------SMAY 335

Query: 170 PNGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVG 229
            + W   GG+ +NP+ + E   GSS+G A S +A       G+ET GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAARCGITA 395

Query: 230 IKPTVGLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIE 280
           ++PT G   R GV+ IS   D +GP CRT AD A +LDAI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 429

BLAST of Chy11G188630 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 103.2 bits (256), Expect = 6.3e-22
Identity = 123/493 (24.95%), Postives = 207/493 (41.99%), Query Frame = 0

Query: 39  LKDLQFAFYQNKLTSTQLVEFYLEQVRRF---NPILNGIIEVNPDALNQASQADLER-KR 98
           ++D  +A+     T  Q+ +  +  +  F    P    +I  + + + + ++A   R ++
Sbjct: 131 IRDYAYAYRSKLTTPLQVAKRIISIIEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQ 190

Query: 99  SSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGK 158
            +P  +S L GI V +KD+I           ++      V +D+ VV+KLR  GAI+ GK
Sbjct: 191 GNP--ISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGK 250

Query: 159 ASLSEWSDFRSYEPPNGWSARGGQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDG 218
           A++ E     +    N  + R       YT     GSSSGSA  VAA + + +LGT+  G
Sbjct: 251 ANMHELGMGTTGNNSNYGTTRNPHDPKRYT----GGSSSGSAAIVAAGLCSAALGTDGGG 310

Query: 219 SILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYD 278
           S+  PS L  + G+K T G T   G +      + +GP+  ++ DA  V  AI G    D
Sbjct: 311 SVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSAD 370

Query: 279 NSTIEAS-----KYIPKGGYGQFLREDGLKGKRIGIVRKLY------DFGD--DDVFYIG 338
              ++ S     K +   G       + +   R+G   K +      D  D  +D+  + 
Sbjct: 371 RYNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDVSSSDISDKCEDILKLL 430

Query: 339 AFEKAFKTMKQGGAILVDNL-TINSFHVIT-GSSS-GEMTALLAEFKISLNAYLKQLVAS 398
           +     K ++    I+V  L  + + HVI+ GS +   +T      K S  +Y  +   +
Sbjct: 431 SNNHGCKVVE----IVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNSKLSYDTRTSFA 490

Query: 399 PIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGRAEKALARLAKLSKEGFERLM 458
             RS S A ++        +L EY   +F + +          A        K G   + 
Sbjct: 491 IFRSFS-ASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDALKNGETNIQ 550

Query: 459 IKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGISFGGLKGFEPRLIEIANG 512
           +   L        L+       GFP +SVP GY+ +GLP G+   G    E  ++ +A  
Sbjct: 551 VTTDLMRFVLAANLL-------GFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAAA 599

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8B7601.9e-15658.63Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY42.0e-8139.45Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C81.1e-7138.02Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
Q8RC401.1e-3929.84Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus s... [more]
Q3AKH03.6e-3828.54Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Synechococcus sp. (strain CC960... [more]
Match NameE-valueIdentityDescription
A0A0A0LLK01.8e-27495.73Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=... [more]
A0A5D3BN384.3e-26894.58Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3C7A84.3e-26894.58putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4... [more]
A0A1S3C6521.9e-26393.81putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4... [more]
E5GC082.0e-26294.66Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147024.30.095.92probable amidase At4g34880 [Cucumis sativus][more]
XP_008457659.10.094.58PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 puta... [more]
XP_008457662.10.093.81PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo][more]
ADN34007.10.094.66amidase [Cucumis melo subsp. melo][more]
KAE8651892.10.093.87hypothetical protein Csa_006820 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G34880.11.0e-13352.26Amidase family protein [more]
AT3G25660.11.5e-3126.64Amidase family protein [more]
AT5G07360.12.9e-2736.21Amidase family protein [more]
AT5G07360.24.0e-2435.34Amidase family protein [more]
AT5G64440.16.3e-2224.95fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 56..455
e-value: 1.2E-68
score: 232.2
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 24..511
e-value: 7.1E-143
score: 479.2
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 33..506
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 19..511
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 19..511

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G188630.1Chy11G188630.1mRNA