Chy11G188510 (gene) Cucumber (hystrix) v1

Overview
NameChy11G188510
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
LocationchrH11: 2518261 .. 2520986 (+)
RNA-Seq ExpressionChy11G188510
SyntenyChy11G188510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCTGTAAACTTGCCGCCGTCCATGACGGTGCTAAAATGGCTAGGATTTGTGTCCGCCGTATGGGTTCAGGCGATTTCTGGCAACAACTACACTTTCTCCAATTACTCCGACGCCTTGAAATCTCTGATGAATCTAACCCAACTTCAACTCAATAATCTGTCCGTGGCCAAAGACGTTGGGAAAGCGTTTGGACTTCTGGCCGGAATCGCTAGTGATAAGTTTCCGACTTGGGTGATTTTGCTTATTGGATCTGTTGAAGGTCTTGTTGGGTATGGCACTCAATGGCTTGTTGTTAGTCGCAGGATCTCTCCTCTTCCTTATTGGCAGGTAACTTTTTTTTTTTTTTTTTTATTTGGTCAATATATATTTGAATCTTTCTTCTCTTCAAGATTTTAATTCTCTTTTCCCTTTTTGTTTTTGTTCATTTCATGTTATGAGCAACACGTCTTTCTTCTTCAACCTTAGCTAATGATATTTAAAGTTCTTTAAAATTAACTAACTTATGGCTACCCCAAAAGGAATATACTCAGAAATGTAAGTTAAAGTTGGTGAAGTTTGAAATATAGAAAACAAATTAAAATGACTCAAATAGTTCAACAGTACGAGACTCAAATCTTTTTACTGAGTGAAAATCTTTCTTTCTTTCTTTAATGTTTTTTAAATTATTAGGCAATATATTTATTTAAGGAGATATCGACATTAAGTTTCAGTTAGCAGGAAAAGTATGGTTTGATGATTAATAAATTGGGAATGAAACACTAATAATCATCCAATTTCAGTTAGCAAAAAACAGTTTAATTAGTTCTTCTTTCTAAAAATCTTCAATTAAACTAAGAAAAAAAATGGCGTTTGTGCTGTTCAGATGTGCATCTTCTTGTGTATGGGTGGAAACAGCACGACATGGATGAACACGGCGGTGCTAGTAACATGCATCAGAAACTTCCGGAAGAATCGCGGCCCAGTTTCCGGCATTCTCAAAGGTTACGTCGGTCTCAGCACCGCCATTTTCACGGATCTCTGTTTTGCTTTGTTTTCCTCCGACCCATCTTCTTTCCTCCTCATGCTCTCCTTGGTCCCTTTCGCCGTCTGTTTCTTCGCCATGTTCTTCCTCCGTGAAATCCCAACTCAAACCACCATCACCGCCGCCGATACCCAACAAGAATCCAATTACTTTTCCGTCTTCAACGCTCTTGCTGTCGTCGTCGCCGTTTATCTCTTATGTTTCGACTTCGTCAAAAACTCCGGCAGACTCATCTCCCAATTATACTCAATTGGGCTTCTGATTCTCCTTGGATCCCCTTTGATTATCCCAATTTACTCATTTTTCAAGAGCTGGAACTCGATTCGGAGCCGGTTGGATCTGGAGGAGCCGCTAGTGAAAGAAGAAGTGGTAACAGGGGCGGTGAAGGAGGAAACAGGGGAAACTGCTGTGATAGAACAGAGAGCGCCGGTGATTGGAGAAGAACATACGATATTTGAAGCGGTGAGAACGATTGATTTCTGGGTGTTGTTCGTGTCGTTTTTGTGTGGAGTTGGAACGGGATTGGCGGTGATGAACAATATGGGTCAGATCGGATTGGCTCTGGGTTACGCGGATGTATCCATGTTCGTTTCTCTTACAAGTATTTGGGGATTTTTTGGCCGGATTCTTTCCGGTACAATTTCCGAGCATTTTCTCAAGTAAGTTCCCCCACGTTCTCTCTTTCTCTCTCTCCCTCGCTTTAGATTTTGGTTTAATTTCTATTTCAAAATATTTTACAATTTTACTTTTATTTTCATTTGATTTTTTAAACTTATAATGGGGAATTTGAGTTTTCAAAATATCTTTTATAGAAAATATCCATTTTGGTCCAACTTTAGAAAGTTTATTCTTTCGAAACCTTTGATATATTGTTTTTGAAAAGATTGAAATGACATTTGCTAGAGATACCCACTTTGGTTGAGATAAGTATTAAATAGGAAAATAGTAACAAAGCCTCTCTTTATTCAACTTTTTTCTTTACATTTAGAGATTAAAAGAAACATGTTCAAATGTTAGACTATTCATTTTGAAAAATAAGTTTATTATAAAGGATGAAAAATAAAAATGTCAAAATAGAAGACCTTCATTCTCAACAAATTTTAAATGGTTGAAAATGATAGTATTAAAAAGTTGAGTTTTTTTCTTTTGAAAAAAGAAGAAAAAAGATATTCTCAAATTAAGATAAATTGAAATGCAATAATATCTTCATGGTTATTATTTCAGGAAAGCAGGCACTCCAAGGCCTCTATGGAACGCAGCGTCTCAAATTCTAATGACCGTTGGGTACGTTTTGATGGCGATGGCGATGCCAGGGTCTCTCTACATCGGTTCAGTCATTGTTGGAATTTGCTACGGCGTTCGTCTCTCCGTCACTGTCCCTACCGCCTCTGAGCTCTTCGGTCTCAAATACTACGGTCTCATCTACAACATTCTCATCCTCAACCTCCCGATTGGCTCCTTCCTATTCTCAGGCCTTCTCGCAGGGTTTCTCTATGACATGGAGGCGACTCCAACCGAGGGCGGGGGGAACACGTGCATCGGAGGACATTGCTATAGAATTGTGTTTCTTGTGATGGCATTGGCTTGTGTGATTGGGTTTGTTTTGGACATTTGGTTGGCGTTTAGAACCAAAGAGCTTTATTCCAAACTTAAAGCGAACAAGAAATCAAAAAAGGTAAACAACAGCAATAACTCTTAG

mRNA sequence

ATGGGTTCTGTAAACTTGCCGCCGTCCATGACGGTGCTAAAATGGCTAGGATTTGTGTCCGCCGTATGGGTTCAGGCGATTTCTGGCAACAACTACACTTTCTCCAATTACTCCGACGCCTTGAAATCTCTGATGAATCTAACCCAACTTCAACTCAATAATCTGTCCGTGGCCAAAGACGTTGGGAAAGCGTTTGGACTTCTGGCCGGAATCGCTAGTGATAAGTTTCCGACTTGGGTGATTTTGCTTATTGGATCTGTTGAAGGTCTTGTTGGGTATGGCACTCAATGGCTTGTTGTTAGTCGCAGGATCTCTCCTCTTCCTTATTGGCAGATGTGCATCTTCTTGTGTATGGGTGGAAACAGCACGACATGGATGAACACGGCGGTGCTAGTAACATGCATCAGAAACTTCCGGAAGAATCGCGGCCCAGTTTCCGGCATTCTCAAAGGTTACGTCGGTCTCAGCACCGCCATTTTCACGGATCTCTGTTTTGCTTTGTTTTCCTCCGACCCATCTTCTTTCCTCCTCATGCTCTCCTTGGTCCCTTTCGCCGTCTGTTTCTTCGCCATGTTCTTCCTCCGTGAAATCCCAACTCAAACCACCATCACCGCCGCCGATACCCAACAAGAATCCAATTACTTTTCCGTCTTCAACGCTCTTGCTGTCGTCGTCGCCGTTTATCTCTTATGTTTCGACTTCGTCAAAAACTCCGGCAGACTCATCTCCCAATTATACTCAATTGGGCTTCTGATTCTCCTTGGATCCCCTTTGATTATCCCAATTTACTCATTTTTCAAGAGCTGGAACTCGATTCGGAGCCGGTTGGATCTGGAGGAGCCGCTAGTGAAAGAAGAAGTGGTAACAGGGGCGGTGAAGGAGGAAACAGGGGAAACTGCTGTGATAGAACAGAGAGCGCCGGTGATTGGAGAAGAACATACGATATTTGAAGCGGTGAGAACGATTGATTTCTGGGTGTTGTTCGTGTCGTTTTTGTGTGGAGTTGGAACGGGATTGGCGGTGATGAACAATATGGGTCAGATCGGATTGGCTCTGGGTTACGCGGATGTATCCATGTTCGTTTCTCTTACAAGTATTTGGGGATTTTTTGGCCGGATTCTTTCCGGTACAATTTCCGAGCATTTTCTCAAGAAAGCAGGCACTCCAAGGCCTCTATGGAACGCAGCGTCTCAAATTCTAATGACCGTTGGGTACGTTTTGATGGCGATGGCGATGCCAGGGTCTCTCTACATCGGTTCAGTCATTGTTGGAATTTGCTACGGCGTTCGTCTCTCCGTCACTGTCCCTACCGCCTCTGAGCTCTTCGGTCTCAAATACTACGGTCTCATCTACAACATTCTCATCCTCAACCTCCCGATTGGCTCCTTCCTATTCTCAGGCCTTCTCGCAGGGTTTCTCTATGACATGGAGGCGACTCCAACCGAGGGCGGGGGGAACACGTGCATCGGAGGACATTGCTATAGAATTGTGTTTCTTGTGATGGCATTGGCTTGTGTGATTGGGTTTGTTTTGGACATTTGGTTGGCGTTTAGAACCAAAGAGCTTTATTCCAAACTTAAAGCGAACAAGAAATCAAAAAAGGTAAACAACAGCAATAACTCTTAG

Coding sequence (CDS)

ATGGGTTCTGTAAACTTGCCGCCGTCCATGACGGTGCTAAAATGGCTAGGATTTGTGTCCGCCGTATGGGTTCAGGCGATTTCTGGCAACAACTACACTTTCTCCAATTACTCCGACGCCTTGAAATCTCTGATGAATCTAACCCAACTTCAACTCAATAATCTGTCCGTGGCCAAAGACGTTGGGAAAGCGTTTGGACTTCTGGCCGGAATCGCTAGTGATAAGTTTCCGACTTGGGTGATTTTGCTTATTGGATCTGTTGAAGGTCTTGTTGGGTATGGCACTCAATGGCTTGTTGTTAGTCGCAGGATCTCTCCTCTTCCTTATTGGCAGATGTGCATCTTCTTGTGTATGGGTGGAAACAGCACGACATGGATGAACACGGCGGTGCTAGTAACATGCATCAGAAACTTCCGGAAGAATCGCGGCCCAGTTTCCGGCATTCTCAAAGGTTACGTCGGTCTCAGCACCGCCATTTTCACGGATCTCTGTTTTGCTTTGTTTTCCTCCGACCCATCTTCTTTCCTCCTCATGCTCTCCTTGGTCCCTTTCGCCGTCTGTTTCTTCGCCATGTTCTTCCTCCGTGAAATCCCAACTCAAACCACCATCACCGCCGCCGATACCCAACAAGAATCCAATTACTTTTCCGTCTTCAACGCTCTTGCTGTCGTCGTCGCCGTTTATCTCTTATGTTTCGACTTCGTCAAAAACTCCGGCAGACTCATCTCCCAATTATACTCAATTGGGCTTCTGATTCTCCTTGGATCCCCTTTGATTATCCCAATTTACTCATTTTTCAAGAGCTGGAACTCGATTCGGAGCCGGTTGGATCTGGAGGAGCCGCTAGTGAAAGAAGAAGTGGTAACAGGGGCGGTGAAGGAGGAAACAGGGGAAACTGCTGTGATAGAACAGAGAGCGCCGGTGATTGGAGAAGAACATACGATATTTGAAGCGGTGAGAACGATTGATTTCTGGGTGTTGTTCGTGTCGTTTTTGTGTGGAGTTGGAACGGGATTGGCGGTGATGAACAATATGGGTCAGATCGGATTGGCTCTGGGTTACGCGGATGTATCCATGTTCGTTTCTCTTACAAGTATTTGGGGATTTTTTGGCCGGATTCTTTCCGGTACAATTTCCGAGCATTTTCTCAAGAAAGCAGGCACTCCAAGGCCTCTATGGAACGCAGCGTCTCAAATTCTAATGACCGTTGGGTACGTTTTGATGGCGATGGCGATGCCAGGGTCTCTCTACATCGGTTCAGTCATTGTTGGAATTTGCTACGGCGTTCGTCTCTCCGTCACTGTCCCTACCGCCTCTGAGCTCTTCGGTCTCAAATACTACGGTCTCATCTACAACATTCTCATCCTCAACCTCCCGATTGGCTCCTTCCTATTCTCAGGCCTTCTCGCAGGGTTTCTCTATGACATGGAGGCGACTCCAACCGAGGGCGGGGGGAACACGTGCATCGGAGGACATTGCTATAGAATTGTGTTTCTTGTGATGGCATTGGCTTGTGTGATTGGGTTTGTTTTGGACATTTGGTTGGCGTTTAGAACCAAAGAGCTTTATTCCAAACTTAAAGCGAACAAGAAATCAAAAAAGGTAAACAACAGCAATAACTCTTAG

Protein sequence

MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDVGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGGNSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLSLVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGRLISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEETGETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSKKVNNSNNS*
Homology
BLAST of Chy11G188510 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 5.1e-67
Identity = 180/553 (32.55%), Postives = 296/553 (53.53%), Query Frame = 0

Query: 5   NLPPSMTVL----KWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 64
           +LPP  T+     KW   V+A+W+QA +G N+ FS YS  LKS++ ++Q++LN L+VA D
Sbjct: 30  HLPPLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASD 89

Query: 65  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLC--M 124
           +GKAFG  +GIA   FP  V+L   +  G VGYG QWLV++  I+ LPY    +FLC  +
Sbjct: 90  LGKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIIT-LPY--SLVFLCCLL 149

Query: 125 GGNSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLM 184
            G S  W NTA  + CIR+F  NR     +   + G+S A+++    A+  S  + +LL+
Sbjct: 150 AGLSICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLL 209

Query: 185 LSLVPFAVCFFAMFFLREIPTQTTITAADTQQ-ESNYFSVFNALAVVVAVYLLCFDFVKN 244
            SLVP  V F A++ +   P+  T    D+++ +S+ F++ N LAV+ + +LL    + +
Sbjct: 210 NSLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLL----LSS 269

Query: 245 SGRLISQLYSIGLLILLGSPLIIPIYSFFKSW------------NSIRSRLDLEEPLVKE 304
           S    ++L  IG ++LL  PL  P+  + + +            +S    L+++E   ++
Sbjct: 270 SSTSSARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQK 329

Query: 305 EVVTGAVKEETGETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNM 364
             V+     E   TA  E     +G+EH+    +  ++FW+ ++++ CG   GL   NN+
Sbjct: 330 TSVSSKTGYEHMGTAK-EGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNL 389

Query: 365 GQIGLALGYADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGY 424
           GQI  +LG    ++ V++ S + FFGR+LS    +   K+    R  W A + +   + +
Sbjct: 390 GQIAQSLGQNSTTL-VTIYSSFSFFGRLLSAA-PDFMHKRFRLTRTGWFAIALLPTPIAF 449

Query: 425 VLMAM--AMPGSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSF 484
            L+A+  +   +L   + ++G+  G   +  V   S+LFG    G+ +NILI N+PIGS 
Sbjct: 450 FLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSL 509

Query: 485 LFSGLLAGFLYDMEA----TPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRT 533
           L+ G +A  +Y+  A    TP       CIG  CY   F+      ++G V  + L  RT
Sbjct: 510 LY-GYIAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRT 569

BLAST of Chy11G188510 vs. ExPASy Swiss-Prot
Match: Q5AXV1 (Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mch1 PE=3 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 4.1e-08
Identity = 117/550 (21.27%), Postives = 207/550 (37.64%), Query Frame = 0

Query: 28  SGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDVGKAFGL-LAGIASDKFPTWVILLIGS 87
           +G+   FS Y   L + +N TQL++N +S+A  +     + LAG   D++    + L   
Sbjct: 94  AGSITAFSLYGPLLLTRLNYTQLRVNEVSIAAGISMYLPVSLAGYLCDRYSPSPLTLFAG 153

Query: 88  VEGLVGYGTQWLVVSRRISP------LPYWQMCIFLCMGGNSTTWMNTAVLVTCIRNF-- 147
           +   +GY     V      P       P+W M +     G +T  M  A + TC +NF  
Sbjct: 154 IAFGLGYSLAAFVYKSGPPPDAGGNGWPFWVMVVAFIAIGVATCSMYLAAVTTCAKNFGR 213

Query: 148 RKNRGPVSGILKGYVGLSTAIFTD-----LCFALFSS-----DPSSFLLMLSLVPFAVCF 207
            K++G +  +     GLS    +      LC  L  S     D   + L L+++   +  
Sbjct: 214 GKHKGIILAVPIAAFGLSGMWQSQVGTYFLCERLKDSNCGDVDVYKYFLFLAILLLTIGV 273

Query: 208 FAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGRLISQLYSI 267
              F LR +         D ++E                        K     + +L   
Sbjct: 274 IGTFALRIV---------DDEEE------------------------KYIDEAVEELERS 333

Query: 268 GLL----ILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEETGETAVIEQ 327
           GLL           +   Y  F   +     +D +   + EE+   A +E+  E     +
Sbjct: 334 GLLAESEFFRPRSEVQAAYGTFSDDHEDNGSVDDQSVTISEELREAARREKEREEEERRK 393

Query: 328 RAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADV------- 387
           +  ++  E  IF A  T+  W L + F    G G A +NN+G I   L            
Sbjct: 394 KNWLLNYETRIFLADHTM--WWLALGFFLVTGPGEAYLNNLGTIVQTLNLDTTAIVDSHP 453

Query: 388 ----SMFVSLTSIWGFFGRILSGTISE--------HFLKKAGTPRP-------------L 447
               S  V++ ++     R+L+G++S+        HF     T  P              
Sbjct: 454 AGLPSTHVTIIALTSTIARLLTGSLSDLFAPTARRHFTVDQETAGPDPFTKQRPALSRLA 513

Query: 448 WNAASQILMTVGYVLMAMAMPGS----LYIGSVIVGICYGVRLSVTVPTASELFGLKYYG 507
           +   S +L+++G++L+A  +P       ++ + +VG+ YG   S+     S ++G++ +G
Sbjct: 514 FLIPSALLLSLGFLLLASPLPTHHPELSHLTTALVGLGYGSIFSLVPIIISVVWGVENFG 573

Query: 508 LIYNILILNLPIGSFLFSGLLAGFLYDMEA--TPTEGGGNTCIGGHCYRIVFLVMALACV 517
             + I+ +  P       G++    Y   A  +PT+ G   C G  C+            
Sbjct: 574 TNWGIVAM-FPAAGAAMWGVIYSRAYQSAADGSPTDDG--QCHGWKCFGF--------WS 597

BLAST of Chy11G188510 vs. ExPASy Swiss-Prot
Match: Q03795 (Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YMR155W PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 8.0e-04
Identity = 119/546 (21.79%), Postives = 205/546 (37.55%), Query Frame = 0

Query: 18  FVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDVGKA-FGLLAGIASDKF 77
           FV    V   +G  Y FS Y+  L S  ++     + LS +  +G +  G+LAGI  D+ 
Sbjct: 22  FVGGNVVALGAGTPYLFSFYAPQLLSKCHIPVSASSKLSFSLTIGSSLMGILAGIVVDRS 81

Query: 78  PTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGGNSTTWMNTAVLVTCIR 137
           P  +  LIGS+   + Y    L      S      + + L   G+ + +  +   V C  
Sbjct: 82  PK-LSCLIGSMCVFIAYLILNLCYKHEWSSTFLISLSLVLIGYGSVSGFYAS---VKCAN 141

Query: 138 -NFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLSLVPFAVCFFAMFFL- 197
            NF ++RG          GLS  +F+ LC  LF  +     + L +    +     F L 
Sbjct: 142 TNFPQHRGTAGAFPVSLYGLSGMVFSYLCSKLFGENIEHVFIFLMVACGCMILVGYFSLD 201

Query: 198 ------------REIPTQTTITAADT-----QQESNYFS--VFNALAVVVAVYLLCFDFV 257
                       +E   Q +    D      +  ++Y    V ++       Y L  +F 
Sbjct: 202 IFSNAEGDDASIKEWELQKSRETDDNIVPLYENSNDYIGSPVRSSSPATYETYALSDNFQ 261

Query: 258 KNSGRLISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEE 317
           +      S+ +++    L   PL+ P        +S  ++ D E+             E 
Sbjct: 262 ET-----SEFFALEDRQLSNRPLLSP--------SSPHTKYDFED-------------EN 321

Query: 318 TGETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMG-----QIG- 377
           T +  V E  A      H +F+++++  F   ++      G GL  + ++G     Q+  
Sbjct: 322 TSKNTVGENSAQKSMRLH-VFQSLKSSTFIGYYIVLGILQGVGLMYIYSVGFMVQAQVST 381

Query: 378 -----LALGYADV-SMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMT- 437
                L +    + S+ V+L S+  F GR+ SG IS+  +KK    R LWN     L+  
Sbjct: 382 PPLNQLPINAEKIQSLQVTLLSLLSFCGRLSSGPISDFLVKKFKAQR-LWNIVIASLLVF 441

Query: 438 -----VGYVLMAMAMPG--------SLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGL 497
                + +   ++  P         ++ + S I G  +GV         ++ FG   Y  
Sbjct: 442 LASNKISHDFSSIEDPSLRASKSFKNISVCSAIFGYSFGVLFGTFPSIVADRFGTNGYST 501

Query: 498 IYNILILNLPIGSFLFSGLLAGFLYDMEATPTEGGGNTCIGGHCYRIVFLVMALACVIG- 514
           ++ +L         +F+ +L     D +A   +  GN   G  CY   F+V         
Sbjct: 502 LWGVLTTGGVFSVSVFTDILG---RDFKANTGDDDGNCKKGVLCYSYTFMVTKYCAAFNL 532

BLAST of Chy11G188510 vs. ExPASy TrEMBL
Match: A0A0A0LLL4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264630 PE=4 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.3e-301
Identity = 534/540 (98.89%), Postives = 537/540 (99.44%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG
Sbjct: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           LVP AVC FAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR
Sbjct: 181 LVPLAVCLFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEETGETA 300
           LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEE GETA
Sbjct: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEEAGETA 300

Query: 301 VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360
           VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF
Sbjct: 301 VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360

Query: 361 VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420
           VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS
Sbjct: 361 VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420

Query: 421 VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480
           VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP
Sbjct: 421 VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480

Query: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSKKVNNSNN 540
           TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSKKVN++N+
Sbjct: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSKKVNSNNS 540

BLAST of Chy11G188510 vs. ExPASy TrEMBL
Match: A0A1S3C607 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497317 PE=4 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.3e-294
Identity = 524/543 (96.50%), Postives = 534/543 (98.34%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+NLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAG+ASD+FPTWVILLIGSVEGLVGYG QWLVVSRRISPL YWQMCIFLCMGG
Sbjct: 61  VGKAFGLLAGMASDRFPTWVILLIGSVEGLVGYGAQWLVVSRRISPLSYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           LVPFAVC FAMFFLREIPTQ TITAADTQQESNYFSVFN LAVVVAVYLLCFDF+KNSGR
Sbjct: 181 LVPFAVCLFAMFFLREIPTQITITAADTQQESNYFSVFNVLAVVVAVYLLCFDFIKNSGR 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEETGETA 300
           LISQLYS+GLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEE VTGAVKEETGE A
Sbjct: 241 LISQLYSMGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEAVTGAVKEETGEIA 300

Query: 301 VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360
           +IEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF
Sbjct: 301 MIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360

Query: 361 VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420
           VSLTSIWGFFGRILSGT+SEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS
Sbjct: 361 VSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420

Query: 421 VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480
           VIVGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP
Sbjct: 421 VIVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480

Query: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLK-ANKKS-KKVNNS 540
           TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLK ANK+S KKVNN+
Sbjct: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKAANKRSTKKVNNN 540

Query: 541 NNS 542
           NNS
Sbjct: 541 NNS 543

BLAST of Chy11G188510 vs. ExPASy TrEMBL
Match: A0A5A7TQ90 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G002430 PE=4 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 6.9e-285
Identity = 513/543 (94.48%), Postives = 523/543 (96.32%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+NLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAG+ASD+FPTWVILLIGSVEGLVGYG QWLV            MCIFLCMGG
Sbjct: 61  VGKAFGLLAGMASDRFPTWVILLIGSVEGLVGYGAQWLV------------MCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           LVPFAVC FAMFFLREIPTQ TITAADTQQESNYFSVFN LAVVVAVYLLCFDF+KNSGR
Sbjct: 181 LVPFAVCLFAMFFLREIPTQITITAADTQQESNYFSVFNVLAVVVAVYLLCFDFIKNSGR 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEETGETA 300
           LISQLYS+GLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEE VTGAVKEETGE A
Sbjct: 241 LISQLYSMGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEAVTGAVKEETGEIA 300

Query: 301 VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360
           +IEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF
Sbjct: 301 MIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360

Query: 361 VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420
           VSLTSIWGFFGRILSGT+SEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS
Sbjct: 361 VSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420

Query: 421 VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480
           VIVGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP
Sbjct: 421 VIVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480

Query: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLK-ANKKS-KKVNNS 540
           TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLK ANK+S KKVNN+
Sbjct: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKAANKRSTKKVNNN 531

Query: 541 NNS 542
           NNS
Sbjct: 541 NNS 531

BLAST of Chy11G188510 vs. ExPASy TrEMBL
Match: A0A6J1G8P6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451839 PE=4 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.8e-262
Identity = 463/541 (85.58%), Postives = 504/541 (93.16%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS  LPPS TVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLM+LTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAGIASD+FPTWVILLIGS+EGL+GYG QWLVVS+RISPLPYWQMC FLC+GG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL+ML+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           +VPFAVC FAMFFLREIP+  T   A+ Q+ES++F+VFNALAVVVAVYLL FDF+KNSG+
Sbjct: 181 VVPFAVCLFAMFFLREIPSTAT---ANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGK 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDL----EEPLVKEEVVTGAVKEET 300
           ++SQL+S+GLLILLGSPL+IPIYSF KSWNS +++LDL    EEPL+KEE    AV++ T
Sbjct: 241 VVSQLFSVGLLILLGSPLVIPIYSFIKSWNSNQTQLDLEAKIEEPLLKEEATAAAVEQST 300

Query: 301 --GETAVIEQRA-PVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360
              E  + E+R  PVIGEEHTIFEA++T+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALG
Sbjct: 301 VVKEEEITEERTPPVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360

Query: 361 YADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMP 420
           YADVSMFVSLTSIWGFFGRIL+GTISEHFLKK+GTPRPLWNAASQILMT+GY+LMAMAMP
Sbjct: 361 YADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMP 420

Query: 421 GSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480
           GSLYIGS++VGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL
Sbjct: 421 GSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480

Query: 481 YDMEATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSK 535
           YDMEATPT GGGNTCIGGHCYRIVF+VMALACVIGFVLDIWLAFRTK LYSKLKANKKS 
Sbjct: 481 YDMEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSN 538

BLAST of Chy11G188510 vs. ExPASy TrEMBL
Match: A0A6J1I5G9 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469786 PE=4 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 1.1e-261
Identity = 461/540 (85.37%), Postives = 502/540 (92.96%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS  LPPS TVLKWLGFVSAVW+QAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWIQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAGIASD+FPTWVILLIGS+EGL+GYG QWLVVS+RISPLPYWQMC FLC+GG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL+ML+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           +VPFAVC FAMFFLREIP+  T   A+ Q+ES++F+VFNALAVVVAVYLL FDF+KNSG+
Sbjct: 181 VVPFAVCLFAMFFLREIPSTAT---ANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGK 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDL----EEPLVKEEVVTGAVKEET 300
           ++SQL+S+GLLILLGSPL+IPIYSFFKSWNS +++LDL    EEPL+KEE +  A +   
Sbjct: 241 VVSQLFSVGLLILLGSPLVIPIYSFFKSWNSNQTQLDLEAKIEEPLLKEEALAAAEQSTV 300

Query: 301 G-ETAVIEQRA-PVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGY 360
             E  + E+R  PVIGEEHTIFEA++T+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALGY
Sbjct: 301 AKEEEIAEERTPPVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGY 360

Query: 361 ADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPG 420
           ADVSMFVSLTSIWGFFGRIL+GTISEHFLKK+GTPRPLWNAASQILMT+GY+LMAMAMPG
Sbjct: 361 ADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPG 420

Query: 421 SLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLY 480
           SLYIGS++VGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLY
Sbjct: 421 SLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLY 480

Query: 481 DMEATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSKK 535
           DMEATP  GGGNTCIGGHCYRIVF+VMALACVIGFVLDIWLAFRTK LYSKLKANKKS K
Sbjct: 481 DMEATPNPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNK 537

BLAST of Chy11G188510 vs. NCBI nr
Match: XP_004147031.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN61919.1 hypothetical protein Csa_006397 [Cucumis sativus])

HSP 1 Score: 1041 bits (2692), Expect = 0.0
Identity = 534/539 (99.07%), Postives = 536/539 (99.44%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG
Sbjct: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           LVP AVC FAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR
Sbjct: 181 LVPLAVCLFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEETGETA 300
           LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEE GETA
Sbjct: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEEAGETA 300

Query: 301 VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360
           VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF
Sbjct: 301 VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360

Query: 361 VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420
           VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS
Sbjct: 361 VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420

Query: 421 VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480
           VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP
Sbjct: 421 VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480

Query: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSKKVNNSN 539
           TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSKKVN++N
Sbjct: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSKKVNSNN 539

BLAST of Chy11G188510 vs. NCBI nr
Match: XP_008457674.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 1020 bits (2637), Expect = 0.0
Identity = 524/543 (96.50%), Postives = 534/543 (98.34%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+NLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAG+ASD+FPTWVILLIGSVEGLVGYG QWLVVSRRISPL YWQMCIFLCMGG
Sbjct: 61  VGKAFGLLAGMASDRFPTWVILLIGSVEGLVGYGAQWLVVSRRISPLSYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           LVPFAVC FAMFFLREIPTQ TITAADTQQESNYFSVFN LAVVVAVYLLCFDF+KNSGR
Sbjct: 181 LVPFAVCLFAMFFLREIPTQITITAADTQQESNYFSVFNVLAVVVAVYLLCFDFIKNSGR 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEETGETA 300
           LISQLYS+GLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEE VTGAVKEETGE A
Sbjct: 241 LISQLYSMGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEAVTGAVKEETGEIA 300

Query: 301 VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360
           +IEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF
Sbjct: 301 MIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360

Query: 361 VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420
           VSLTSIWGFFGRILSGT+SEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS
Sbjct: 361 VSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420

Query: 421 VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480
           VIVGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP
Sbjct: 421 VIVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480

Query: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKA-NKKS-KKVNNS 540
           TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKA NK+S KKVNN+
Sbjct: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKAANKRSTKKVNNN 540

BLAST of Chy11G188510 vs. NCBI nr
Match: KAA0045723.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 987 bits (2551), Expect = 0.0
Identity = 513/543 (94.48%), Postives = 523/543 (96.32%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+NLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAG+ASD+FPTWVILLIGSVEGLVGYG QWLV            MCIFLCMGG
Sbjct: 61  VGKAFGLLAGMASDRFPTWVILLIGSVEGLVGYGAQWLV------------MCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           LVPFAVC FAMFFLREIPTQ TITAADTQQESNYFSVFN LAVVVAVYLLCFDF+KNSGR
Sbjct: 181 LVPFAVCLFAMFFLREIPTQITITAADTQQESNYFSVFNVLAVVVAVYLLCFDFIKNSGR 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEETGETA 300
           LISQLYS+GLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEE VTGAVKEETGE A
Sbjct: 241 LISQLYSMGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEAVTGAVKEETGEIA 300

Query: 301 VIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360
           +IEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF
Sbjct: 301 MIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 360

Query: 361 VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420
           VSLTSIWGFFGRILSGT+SEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS
Sbjct: 361 VSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 420

Query: 421 VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480
           VIVGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP
Sbjct: 421 VIVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATP 480

Query: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKA-NKKS-KKVNNS 540
           TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKA NK+S KKVNN+
Sbjct: 481 TEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKAANKRSTKKVNNN 531

BLAST of Chy11G188510 vs. NCBI nr
Match: XP_038902519.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 964 bits (2492), Expect = 0.0
Identity = 492/545 (90.28%), Postives = 512/545 (93.94%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+ L PS T LKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLKLRPSTTALKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAGIA D+FPTW ILLIGS+EGL+GYG QWLVVS+RISPLPYWQMCIFLC+GG
Sbjct: 61  VGKAFGLLAGIACDRFPTWAILLIGSLEGLIGYGVQWLVVSQRISPLPYWQMCIFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSS+PS FLLML+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSNPSPFLLMLA 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
            VPFAVC FAMFFLREIP   + TAADTQQES+YFSVFNALAVVVAVYLLCFDF+KNSGR
Sbjct: 181 FVPFAVCLFAMFFLREIPPPPSTTAADTQQESDYFSVFNALAVVVAVYLLCFDFIKNSGR 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLE----EPLVKEEVVTGAVKEET 300
           LISQL+S GLL LLGSPL+IPIYSFFKSWNS R R DLE    EPL+KEEVVT AVKEE 
Sbjct: 241 LISQLFSFGLLFLLGSPLVIPIYSFFKSWNSNRDRSDLEARIEEPLLKEEVVTEAVKEEA 300

Query: 301 GETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
           GE A  EQR PVIGEEHTIFEAV+TIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 GEIAKEEQRLPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSL 420
           VSMFVSL SIWGFFGRILSGT+SEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSL
Sbjct: 361 VSMFVSLISIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSL 420

Query: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480
           YIGSVIVGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSVIVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

Query: 481 EATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSKKVN 540
           EATP EGGGNTCIGGHCYRIVFLVMALAC+IGF LDIWLAFRTKELYSKLKANKK+KK N
Sbjct: 481 EATPIEGGGNTCIGGHCYRIVFLVMALACIIGFALDIWLAFRTKELYSKLKANKKTKKGN 540

BLAST of Chy11G188510 vs. NCBI nr
Match: XP_023532459.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 463/541 (85.58%), Postives = 503/541 (92.98%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS  LPPS TVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFGLLAGIASD+FPTWVILLIGS+EGL+GYG QWLVVS+RISPLPYWQMC FLC+GG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL+ML+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           +VPFAVC FAMFFLREIP+  T   A+ Q+ES++F+VFNALAVVVAVYLL FDF+KNSG+
Sbjct: 181 VVPFAVCLFAMFFLREIPSTAT---ANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGK 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDLE----EPLVKEEVVTGAVKEET 300
           ++SQL+S+GLLILLGSPL+IPIYS FKSWNS +++LDLE    EPL+KEE    A ++ T
Sbjct: 241 VVSQLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLLKEEAAAAAAEQST 300

Query: 301 --GETAVIEQRAP-VIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360
              E  + E+R P VIGEEHTIFEA++T+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALG
Sbjct: 301 VVKEEEITEERTPPVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360

Query: 361 YADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMP 420
           YADVSMFVSLTSIWGFFGRIL+GTISEHFLKK+GTPRPLWNAASQILMT+GY+LMAMAMP
Sbjct: 361 YADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMP 420

Query: 421 GSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480
           GSLYIGS++VGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL
Sbjct: 421 GSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480

Query: 481 YDMEATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSK 534
           YDMEATPT GGGNTCIGGHCYRIVF+VMALACVIGFVLDIWLAFRTK LYSKLKANKKS 
Sbjct: 481 YDMEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSN 538

BLAST of Chy11G188510 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 758.1 bits (1956), Expect = 5.0e-219
Identity = 390/551 (70.78%), Postives = 458/551 (83.12%), Query Frame = 0

Query: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MG      S + LKWLGFV+AVWVQ+ISGNNYTFSNYS ALKSLMNL QL+LNNLSVAKD
Sbjct: 1   MGFQRSISSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120
           VGKAFG+LAG+ASD+ PT VILLIG  EGL+GYG QWLVVSR I P+PYWQMCIFLCMGG
Sbjct: 61  VGKAFGILAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180
           NSTTWMNTAVLVTCIRNFR+NRGPVSGILKGYVGLSTAIFTDLC ALFS+DP+SFL++L+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLA 180

Query: 181 LVPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240
           +VPFAVC  A+FFLREIP  +  +AA+  +E+ YF++FN +AVVVAVYL  +D +     
Sbjct: 181 VVPFAVCLTAVFFLREIPPAS--SAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKTG 240

Query: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWN-----SIRSRLDLEEPLV--------KEEV 300
           + S  ++  LL LL SP+ IP +SF KS N      +  R  ++EPL+        KE +
Sbjct: 241 VFSVAFASILLFLLASPIAIPFHSFIKSLNYGEQDDLEGR--IQEPLLRSEIAAAEKEVI 300

Query: 301 VTGAVKEETGETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQ 360
           V  AV  E  E    +++ PV+GE+HTI EAV T+DFWVLFVSFLCGVGTGLAVMNNMGQ
Sbjct: 301 VVAAVAAEEEEK---KRKKPVLGEDHTIMEAVLTVDFWVLFVSFLCGVGTGLAVMNNMGQ 360

Query: 361 IGLALGYADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVL 420
           IGLALGY +VS+FVS+TSIWGFFGRILSGT+SE+FLKKAGTPRPLWNAASQILM VGY+L
Sbjct: 361 IGLALGYTNVSIFVSMTSIWGFFGRILSGTLSEYFLKKAGTPRPLWNAASQILMAVGYIL 420

Query: 421 MAMAMPGSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSG 480
           MA+A+P SLYIGS++VG+CYGVRL++TVPTASELFGLKYYGLIYNIL+LNLP+GSFLFSG
Sbjct: 421 MALAVPNSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILVLNLPLGSFLFSG 480

Query: 481 LLAGFLYDMEATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLK 539
           LLAGFLYD EATPT GGGNTC+G HCYR++F+VMALA VIG  LD+ LA+RTKE+Y+K+ 
Sbjct: 481 LLAGFLYDAEATPTPGGGNTCVGAHCYRLIFIVMALASVIGVGLDLVLAYRTKEIYAKIH 540

BLAST of Chy11G188510 vs. TAIR 10
Match: AT4G34950.1 (Major facilitator superfamily protein )

HSP 1 Score: 752.7 bits (1942), Expect = 2.1e-217
Identity = 387/562 (68.86%), Postives = 456/562 (81.14%), Query Frame = 0

Query: 2   GSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDV 61
           G+ +   S + LKWLGFV+AVWVQ+ISGNNYTFSNYS ALKSLMNLTQL+LN+LSVAKDV
Sbjct: 4   GTSSSSSSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDV 63

Query: 62  GKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGGN 121
           GKAFG+LAG+ASD+  T VILLIGS EGL+GYG QWLVVSR I P+PYWQMC+FLCMGGN
Sbjct: 64  GKAFGILAGLASDRLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYWQMCVFLCMGGN 123

Query: 122 STTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLSL 181
           STTWMNTAVLVTCIRNFR+NRGPVSGILKGYVGLSTAIFTDLC ALFSSDP+SFL++LS+
Sbjct: 124 STTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPASFLVLLSV 183

Query: 182 VPFAVCFFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGRL 241
           VPFAVC  A+FFLREIP  TT   A+  +ES YF+VFN +AVVVAVYL  +D +      
Sbjct: 184 VPFAVCLTAVFFLREIPPSTTF--AEDNEESKYFAVFNIVAVVVAVYLQSYDIIGIKTGA 243

Query: 242 ISQLYSIGLLILLGSPLIIPIYSFFKS----WNSIRSRLDLEEPLVK-------EEVVTG 301
            S  ++  LLILL SP+ +P ++F +S       +  R+D  EPL++       EE + G
Sbjct: 244 FSIAFASILLILLASPVAVPFHAFIRSKVHDEQDVEGRID--EPLLRSGSEIEVEETIVG 303

Query: 302 AV------------------KEETGETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFL 361
           A                   +E  G     E++ PV+GEEHTI EA+ T+DFWVLFVSFL
Sbjct: 304 AAAAADNELPPSLKPLSNEEEENHGTIVTTEKKRPVLGEEHTIMEAMLTVDFWVLFVSFL 363

Query: 362 CGVGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPL 421
           CGVGTGLAVMNNMGQIGLALGY DVS+FVS+TSIWGFFGRILSGTISEHF+KKAGTPRPL
Sbjct: 364 CGVGTGLAVMNNMGQIGLALGYTDVSIFVSMTSIWGFFGRILSGTISEHFIKKAGTPRPL 423

Query: 422 WNAASQILMTVGYVLMAMAMPGSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYN 481
           WNAA+QI+M VGY+LMA+A+PGSLYIGS++VG+CYGVRL++TVPTASELFGLKYYGLIYN
Sbjct: 424 WNAAAQIIMAVGYLLMALALPGSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYN 483

Query: 482 ILILNLPIGSFLFSGLLAGFLYDMEATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLD 535
           ILILN+P+GSFLFSGLLAG LYD EATPT GGGNTC+G HC+RIVF+VMA A +IG  LD
Sbjct: 484 ILILNMPLGSFLFSGLLAGLLYDAEATPTPGGGNTCVGAHCFRIVFIVMAFASIIGVGLD 543

BLAST of Chy11G188510 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 342.0 bits (876), Expect = 8.5e-94
Identity = 200/559 (35.78%), Postives = 310/559 (55.46%), Query Frame = 0

Query: 14  KWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDVGKAFGLLAGIAS 73
           +WL FV+A+W+Q+ +G  Y F + S  +KS +N  Q +L+ L VAKD+G + G +AG  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 74  DKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGGNSTTWMNTAVLVT 133
           +  P W  LL+G+V+ L+GYG  WL+V+ R   LP W MC+ + +G N  T+ NT  LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 134 CIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLSLVPFAVCFFAMFF 193
            ++NF K+RGPV GILKG+ GL  AI + +   + SS+P+S +LM+++ P  V    MFF
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFF 195

Query: 194 LREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGRLISQLYSIGLLIL 253
           +R +     I   D    +  + V   LA  +   +L  D V  S  +I+ +++I L ++
Sbjct: 196 IRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVIT-VFTIVLFVI 255

Query: 254 LGSPLIIPIY-SFFKSWNS---------IRSRLDLEEPLVKEEVVTGAVKEE-------- 313
           L  P+++PI  SFF   N          +  R D E  L   +++   V++E        
Sbjct: 256 LVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLL 315

Query: 314 ---------------------TGETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCG 373
                                 G   V  +R P  GE+ T+ +A+   DFW++F S L G
Sbjct: 316 PASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLG 375

Query: 374 VGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWN 433
            G+GL V++N+GQ+  +LGY +  + VS+ SIW F GRI  G  SE  ++    PRP+  
Sbjct: 376 SGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVAM 435

Query: 434 AASQILMTVGYVLMAMAMPGSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNIL 493
           A +Q++M+VG++  A   PG++YIG++++G+ YG   ++   TASELFGLK +G +YN L
Sbjct: 436 AVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNFL 495

Query: 494 ILNLPIGSFLFSGLLAGFLYDMEATPTEGG-------GNTCIGGHCYRIVFLVMALACVI 527
            L  P GS +FSG++A  +YD EA     G          C G  C+ +  L+M+  C+I
Sbjct: 496 TLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSGFCII 555

BLAST of Chy11G188510 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 338.2 bits (866), Expect = 1.2e-92
Identity = 208/570 (36.49%), Postives = 315/570 (55.26%), Query Frame = 0

Query: 14  KWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDVGKAFGLLAGIAS 73
           +WL FV+A+W+Q+ +G  Y F + S  +KS +N  Q QL+ L VAKD+G + G LAG  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLS 75

Query: 74  DKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGGNSTTWMNTAVLVT 133
           +  P W  LL+GSV+ LVGYG  WL+V+ R   LP W MCI + +G N  T+ NTA LV+
Sbjct: 76  EILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVS 135

Query: 134 CIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLSLVPFAVCFFAMFF 193
            ++NF K+RGPV GILKG+ GL  AI + +   + SSD +S + M+++ P  V    MFF
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPLMFF 195

Query: 194 LREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCF----DFVKNSGRLISQLYSIG 253
           +R +     I ++D    +  F+V  A+ +++A YL+      DF+  S  +I   +++ 
Sbjct: 196 IRPVGGHRQIRSSD----ATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIII-AFTVV 255

Query: 254 LLILLGSPLIIPIYSFFKSWNSIRSRLD-LEEPLVKE----------------EVVTGAV 313
           L  +L  P+ IPI +    + +     D LEEPL+ +                E++   V
Sbjct: 256 LFAILLVPIFIPIAT--SCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEV 315

Query: 314 KEE-----------------------------TGETAVIEQRAPVIGEEHTIFEAVRTID 373
           ++E                              G   V  +R P  GE+ T+ +A+   D
Sbjct: 316 EDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKAD 375

Query: 374 FWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFGRILSGTISEHFL 433
           FW++F S L G G+GL V++N+GQ+  +LGY +  +FVS+ SIW F GRI  G  SE  +
Sbjct: 376 FWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSELIV 435

Query: 434 KKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGSVIVGICYGVRLSVTVPTASELFG 493
           +    PRP+  A +Q++M+VG++  A   PG+++IG++++G+ YG   ++   TASELFG
Sbjct: 436 RDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFG 495

Query: 494 LKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATPTEGGG-------NTCIGGHCYRI 527
           LK +G +YN L L  P GS +FSGL+A  +YD EA     G          C G  CY +
Sbjct: 496 LKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSICYFL 555

BLAST of Chy11G188510 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 325.9 bits (834), Expect = 6.3e-89
Identity = 193/531 (36.35%), Postives = 306/531 (57.63%), Query Frame = 0

Query: 14  KWLGFVSAVWVQAISGNNYTF-SNYSDALKSLMNLTQLQLNNLSVAKDVGKAFGLLAGIA 73
           +WL FV A+WVQ+++G  Y F  + S A+K+ +   Q Q+  L VAK++G A G ++G  
Sbjct: 16  RWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGAL 75

Query: 74  SDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGGNSTTWMNTAVLV 133
           S+  P+WV+LL+G+ + L GYG  WLVV+ ++  LP W + + + +G N  T+ NTA LV
Sbjct: 76  SEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLV 135

Query: 134 TCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLSLVPFAVCFFAMF 193
           +CI NF ++RGPV GILKG+ GLS AI T +      S  SS +LM++L P  V    +F
Sbjct: 136 SCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLF 195

Query: 194 FLR--EIPTQTTITAADTQQESNYFSVFNALAVVVAVY---LLCFDFVKNSGRLISQLYS 253
            +R  E   +T + + D +     F       VV+AVY   LL    V +  + I     
Sbjct: 196 IVRPVERSCRTNLRSDDLR-----FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSG 255

Query: 254 IGLLILLGSPLIIPIYSFFKSWNSIRSRLDLEEPLVKEEVVTGAVKEETGETAV------ 313
             L+I +  P+++P  S F S N++ S        VK E  T  V +    T +      
Sbjct: 256 AILVIFMVVPVLVPFSSVFISGNNVTS--------VKPEEGTSNVDQHEARTLIERSDRP 315

Query: 314 -IEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMF 373
             ++RAP IGE+ T+ +A+   DFW++F+S + GVG+G+ +++N+GQI  +LGY++  +F
Sbjct: 316 PEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIF 375

Query: 374 VSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSLYIGS 433
           VSL SI  F GR+  G  SE  ++K   PR L  +  Q +M++G +  A+  PG +Y+ +
Sbjct: 376 VSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVT 435

Query: 434 VIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEA-- 493
           +++G+ YG   ++   + S++FGLK +G +YN  I  LPIGSF+FSG++A  +YD  A  
Sbjct: 436 IVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARK 495

Query: 494 ---TPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKL 527
                TE     C G  CY +   +M++ C++  VL + + +RT++ Y +L
Sbjct: 496 QAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRL 533

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E15.1e-6732.55Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q5AXV14.1e-0821.27Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / ... [more]
Q037958.0e-0421.79Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATC... [more]
Match NameE-valueIdentityDescription
A0A0A0LLL45.3e-30198.89Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G26463... [more]
A0A1S3C6071.3e-29496.50protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497317 PE=4 ... [more]
A0A5A7TQ906.9e-28594.48Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1G8P62.8e-26285.58protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I5G91.1e-26185.37protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
Match NameE-valueIdentityDescription
XP_004147031.10.099.07protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN61919.1 hypothetical pr... [more]
XP_008457674.10.096.50PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo][more]
KAA0045723.10.094.48protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa][more]
XP_038902519.10.090.28protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
XP_023532459.10.085.58protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G16660.15.0e-21970.78Major facilitator superfamily protein [more]
AT4G34950.12.1e-21768.86Major facilitator superfamily protein [more]
AT5G14120.18.5e-9435.78Major facilitator superfamily protein [more]
AT3G01930.21.2e-9236.49Major facilitator superfamily protein [more]
AT5G50630.16.3e-8936.35Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 10..211
e-value: 6.5E-7
score: 30.9
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 321..540
e-value: 5.7E-10
score: 40.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 15..520
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 14..262
e-value: 1.2E-93
score: 313.2
NoneNo IPR availablePANTHERPTHR21576:SF105PROTEIN NUCLEAR FUSION DEFECTIVE 4-LIKEcoord: 10..539
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 10..539
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 13..508
e-value: 3.40677E-128
score: 377.747

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G188510.1Chy11G188510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane