Chy11G186210 (gene) Cucumber (hystrix) v1

Overview
NameChy11G186210
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPectin lyase-like superfamily protein
LocationchrH11: 468180 .. 471634 (+)
RNA-Seq ExpressionChy11G186210
SyntenyChy11G186210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAGTGATAAATCAAAATTCTATTTCGGCCCTCCGATCAATCCCTCCTGTTCTTCCCTTCTCCTTCTCTTCATCTTCCTCGCTATTCTCACCTTCCAAATCGCCACCAGAACTCCCCCTTTCCCCTTCTGGCTCCCACCCTCTGATTTCCAACTCCCCACCTGCTCCGCCTTCTTCCGCCCAGATGCTACGGCGGCGGTTGTCATGTCGATCACGGATTTCGGAGGAGTAGGGGACGGGAAGACTTCCAACACGGAAGCGTTTCGGAAGGCCATTCAGTACATGCGGCGGTTCGGCGACAAAGGCGGGTCTAAACTGACCGTCCCTAAGGGCAGTTGGGTCACGGGCAGCTTTAATCTCACCAGTAATTTTACCCTCTTTCTCCAACGTGGCGCTCTTATTTTAGCCTCCCAGGTCAGCCTTTTTTTTTAACCCTAAACCCCTAAAATTACTTATGGTTAAACTAGATAGTTATTAGAAAATTAAACGGTAATTTTAAGCTTGTCTTTTGTAATTTGTTTTTTTGTTTTAAATATTTATGTGTTTAAGACTTTGTGTGGTGTTTCTTTTAAACTGTTGGGAAATTTTACTTTTATTGTTTTTTATTGTTTTAATGAGTTTAGAAAGGACAAATTCTTTGTGATTTTGGGGAAGAAGAAAATGCCAAATGGGATTTCATAACTTAGCAGAAAAGGTAAAATCAGAGTGATGACTCTGGCGGTGTCACCTAAGTTCTTATTGCATTTTTAAACCTAAAAACATAGCAAGTGGGATCCACCATCTCTCTCTCTCTCTCTATATATATATATCCAATATTTCCATCCAAATTGATTAAATAATAATAGATAATAAATATTTTTGCATGCAAGAAAAATGTAATATCTGAACTTATTTATAGTGAAAATGTGAATTGATAATAATATTATTTTTTAAAGAAAAAAAAAAAAGCAATAAAACAAGTAAAGTTGTAAGTAACCAAAAAGTAATGATAATAAATAATAATAAAGCAGTAAAATTATGAGAAAAAAAAAGGTTGTATATGCTAATGATTTATTAGCACTATGAATTATCATTTAATGCATTCATTAGTGCTTTTGAAACTTATTTATATTAGTGTTTGGGATTTCCCATTAACAAAATTCCTTAGAAAAAATAAAATAAAATTAAAGTTGTGATTCCTTCCCCCAAGGTGTGTAAATGTAAACAGAGCTTTTAAGATAACTGGTTGACTTTTTTTTTTCTTTTTTGGTGGGTATTATTTGAAATTGAATAAGCTTTGGTTTAATCAATAAAATAAATTATCAAATCAAATTTGTTAATTATGAATTTATAAATCTTTGCTTTTAAAAATCATGGTGGCATGTTATTGTCAAAAGTTTAGTGTGCATTTTTTTTAATATTTGTTTGAAATTAAAAAAAAAAAAAAAAAGGATCCAGATGAGTGGCCCATTATAGAGCCACTTCCTTCTTATGGAAGAGGAAGAGAGAGATTGGGAGGAAGACACATCAGCCTTATTCATGGCAATGCCCTTTCAAATGTTGTAATTACAGGTACTTTCACTTTTCTTTATCTTTTCTTATTTTTTTGTTTTTGGCTTCATCTTCATTGCTAGGATTTTATATTCATGAGGATTCTTATCTTGGGTTTTGTAAAGTAATGGAATCAAACTTTGATCATGACTAAGCAATTAGCTAGGGAACCATGATAATGTTAAAAATTAAAAAAAGGGATTTAGGATCTAAGAATATTATTTCTCTTTCTCAAAATCATGCTTTATTTTCTATGGTGTGTTTTTATGTTTGGGGTGGGAATGATAAAGAACTGAATCTTAGTATACCATTTCAACTGTTTTTAAAAGTGAAATTGGCAATAAGGAGTTTTTTTTGTAAGGGCACTTGACCTTTTAGTTCATTTTGGTTGTGGAGAAGATCATTATAGTATTTTATGTTAAGGGGTCACTTGAGATGAGAAAAGTTAGGTATTTCAATTTAAAGCATAAAATCTACCTTCTAAGACTAACTTGACTTACCCATTTTCTTTCTTTGATTTTCTTGTTTTTTACTGTAGCCAAATACCACTAGGTCACCAATGTGATCAGTATAGCTCATTTGGTTAAAACATATATAAATTTAACCAAAATGTCAGATGTTTGAAACTCACCCTTACATATTGTTGAACTAAAAAGAATATGAGTAGAGCCAAGTGATAAAAACTAGGAATAAGGTGTTCTACCTACCTTTAAAAAGTATCAAATGGGATCATATCTTTTGATGTACATTAGCTTTGTGTATCAAAACTGGAAATAGTGTGAACTCTTCTTCTCGTTTAGCTTTGATGGCACTCGGGTTCGAGAATGTTCTAAATCATGAAAATCTAATGAGTTTGTATGTTGTTTTTTCAGGAGAGAATGGAACCATTGATGGGCAAGGCAAGATGTGGTGGGAGCTTTGGTGGAATAGAACATTAAATCACACAAGGGGCCATCTTGTAGAGCTCATCAACTCTCACAATATTCTCATCTCCAATCTCACCTTCAAAAATTCCCCCTTTTGGACAATTCATCCCGTCTATTGCAGGTTTCATTTTCAACAATTATCTACCCTTTCCGATTATATACACTTGTGTTTGGTTCTTGAACTTTTGTTTTTCAGTAATGTTGTTATCAAAGACATGACGATCTTGGCTCCGCTCAATGCCCCCAACACAGATGGTATCGATCCAGGTATTTTGCTTCTTTCTTGGAGGAGGTATGAGAAACAAATGTAAAACAATGCTGGAGTTATCTAAATTCCAAATGCATCTTTATTATGGTGTAGATTCTAGTACCAATGTTTGTATCGAGGACTGTTATATCGAAAGCGGAGATGATCTAGTAGCAGTGAAGAGTGGATGGGATCAATATGGAATCAACCTTGCACGCCCAAGCTTGAACATTGTCATAAGGCGAGTTTCTGGCACCACCCCTACCTGTTCTGGTGTTGGAATAGGAAGTGAGATGTCGGGTGGAATATCAAACATTACGATTGAGGATTTAAATGTCTGGGATTCGGCAGCTGGCATTCGAATCAAATCTGACCAAGGTAGAGGAGGATACATTGCAAATGTTAGTATAACTAACTTTGTAATGAACAGAGTGAAAATGGCTATAAGGTTTAGCAGGGGTTCAAATGACCATCCTGATGAACAATTTGATCCAAAGGCAGTTCCTAAAGTGAAGGGCATTTTCATTACCAATCTCATGAGCTTAAATTCGACAAAGGCTCCGGTTCTTTACGGGATCGCAGGCACATCATATGATGGTGTATGTATGAAAAATGTGACCATTCTCGGTCTCGCTCCCTCTGCAAAATGGCATTGTGCTTTTGTCTCGGGTTTTAGCACTTCAGTCTTTCCTATGCCATGTCCTCAGTTGCAGAACACCACATTTTCATCGTTGTGCTCGTCATTTTAA

mRNA sequence

ATGGACAGTGATAAATCAAAATTCTATTTCGGCCCTCCGATCAATCCCTCCTGTTCTTCCCTTCTCCTTCTCTTCATCTTCCTCGCTATTCTCACCTTCCAAATCGCCACCAGAACTCCCCCTTTCCCCTTCTGGCTCCCACCCTCTGATTTCCAACTCCCCACCTGCTCCGCCTTCTTCCGCCCAGATGCTACGGCGGCGGTTGTCATGTCGATCACGGATTTCGGAGGAGTAGGGGACGGGAAGACTTCCAACACGGAAGCGTTTCGGAAGGCCATTCAGTACATGCGGCGGTTCGGCGACAAAGGCGGGTCTAAACTGACCGTCCCTAAGGGCAGTTGGGTCACGGGCAGCTTTAATCTCACCAATGAGTGGCCCATTATAGAGCCACTTCCTTCTTATGGAAGAGGAAGAGAGAGATTGGGAGGAAGACACATCAGCCTTATTCATGGCAATGCCCTTTCAAATGTTGTAATTACAGGAGAGAATGGAACCATTGATGGGCAAGGCAAGATGTGGTGGGAGCTTTGGTGGAATAGAACATTAAATCACACAAGGGGCCATCTTGTAGAGCTCATCAACTCTCACAATATTCTCATCTCCAATCTCACCTTCAAAAATTCCCCCTTTTGGACAATTCATCCCGTCTATTGCAGGTTTCATTTTCAACAATTATCTACCCTTTCCGATTATATACACTTGTGTTTGGTTCTTGAACTTTTGTTTTTCAGTAATGTTGTTATCAAAGACATGACGATCTTGGCTCCGCTCAATGCCCCCAACACAGATGGTATCGATCCAGATTCTAGTACCAATGTTTGTATCGAGGACTGTTATATCGAAAGCGGAGATGATCTAGTAGCAGTGAAGAGTGGATGGGATCAATATGGAATCAACCTTGCACGCCCAAGCTTGAACATTGTCATAAGGCGAGTTTCTGGCACCACCCCTACCTGTTCTGGTGTTGGAATAGGAAGTGAGATGTCGGGTGGAATATCAAACATTACGATTGAGGATTTAAATGTCTGGGATTCGGCAGCTGGCATTCGAATCAAATCTGACCAAGGTAGAGGAGGATACATTGCAAATGTTAGTATAACTAACTTTGTAATGAACAGAGTGAAAATGGCTATAAGGTTTAGCAGGGGTTCAAATGACCATCCTGATGAACAATTTGATCCAAAGGCAGTTCCTAAAGTGAAGGGCATTTTCATTACCAATCTCATGAGCTTAAATTCGACAAAGGCTCCGGTTCTTTACGGGATCGCAGGCACATCATATGATGGTGTATGTATGAAAAATGTGACCATTCTCGGTCTCGCTCCCTCTGCAAAATGGCATTGTGCTTTTGTCTCGGGTTTTAGCACTTCAGTCTTTCCTATGCCATGTCCTCAGTTGCAGAACACCACATTTTCATCGTTGTGCTCGTCATTTTAA

Coding sequence (CDS)

ATGGACAGTGATAAATCAAAATTCTATTTCGGCCCTCCGATCAATCCCTCCTGTTCTTCCCTTCTCCTTCTCTTCATCTTCCTCGCTATTCTCACCTTCCAAATCGCCACCAGAACTCCCCCTTTCCCCTTCTGGCTCCCACCCTCTGATTTCCAACTCCCCACCTGCTCCGCCTTCTTCCGCCCAGATGCTACGGCGGCGGTTGTCATGTCGATCACGGATTTCGGAGGAGTAGGGGACGGGAAGACTTCCAACACGGAAGCGTTTCGGAAGGCCATTCAGTACATGCGGCGGTTCGGCGACAAAGGCGGGTCTAAACTGACCGTCCCTAAGGGCAGTTGGGTCACGGGCAGCTTTAATCTCACCAATGAGTGGCCCATTATAGAGCCACTTCCTTCTTATGGAAGAGGAAGAGAGAGATTGGGAGGAAGACACATCAGCCTTATTCATGGCAATGCCCTTTCAAATGTTGTAATTACAGGAGAGAATGGAACCATTGATGGGCAAGGCAAGATGTGGTGGGAGCTTTGGTGGAATAGAACATTAAATCACACAAGGGGCCATCTTGTAGAGCTCATCAACTCTCACAATATTCTCATCTCCAATCTCACCTTCAAAAATTCCCCCTTTTGGACAATTCATCCCGTCTATTGCAGGTTTCATTTTCAACAATTATCTACCCTTTCCGATTATATACACTTGTGTTTGGTTCTTGAACTTTTGTTTTTCAGTAATGTTGTTATCAAAGACATGACGATCTTGGCTCCGCTCAATGCCCCCAACACAGATGGTATCGATCCAGATTCTAGTACCAATGTTTGTATCGAGGACTGTTATATCGAAAGCGGAGATGATCTAGTAGCAGTGAAGAGTGGATGGGATCAATATGGAATCAACCTTGCACGCCCAAGCTTGAACATTGTCATAAGGCGAGTTTCTGGCACCACCCCTACCTGTTCTGGTGTTGGAATAGGAAGTGAGATGTCGGGTGGAATATCAAACATTACGATTGAGGATTTAAATGTCTGGGATTCGGCAGCTGGCATTCGAATCAAATCTGACCAAGGTAGAGGAGGATACATTGCAAATGTTAGTATAACTAACTTTGTAATGAACAGAGTGAAAATGGCTATAAGGTTTAGCAGGGGTTCAAATGACCATCCTGATGAACAATTTGATCCAAAGGCAGTTCCTAAAGTGAAGGGCATTTTCATTACCAATCTCATGAGCTTAAATTCGACAAAGGCTCCGGTTCTTTACGGGATCGCAGGCACATCATATGATGGTGTATGTATGAAAAATGTGACCATTCTCGGTCTCGCTCCCTCTGCAAAATGGCATTGTGCTTTTGTCTCGGGTTTTAGCACTTCAGTCTTTCCTATGCCATGTCCTCAGTTGCAGAACACCACATTTTCATCGTTGTGCTCGTCATTTTAA

Protein sequence

MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFFRPDATAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFNLTNEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFHFQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQLQNTTFSSLCSSF*
Homology
BLAST of Chy11G186210 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.1e-100
Identity = 191/430 (44.42%), Postives = 265/430 (61.63%), Query Frame = 0

Query: 71  SITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFNLT-------- 130
           S+ DFGGVGDG+T NT+AF+ A+  + ++G +GG++L VP G W+TGSF+LT        
Sbjct: 64  SLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLH 123

Query: 131 -----------NEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGENGTIDGQGK 190
                      ++WP+I+PLPSYGRGR+   GR+ SLI G  L++V+ITG+NGTIDGQG 
Sbjct: 124 RDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGG 183

Query: 191 MWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFHFQQLSTLSDY 250
           +WW+ +    L +TR +L+EL+ S +I ISNLT  NSP W +HPVY R            
Sbjct: 184 LWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSR------------ 243

Query: 251 IHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKS 310
                        N++I+ +TILAP+ +PNTDGI+PDS TN  IEDCYI SGDD VAVKS
Sbjct: 244 -------------NILIQGITILAPVRSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKS 303

Query: 311 GWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRI 370
           GWD+YGI    P+  +VIRR++  +P  + + +GSEMSGGI ++  ED+   +S +GIRI
Sbjct: 304 GWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRI 363

Query: 371 KSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSL 430
           K+  GRGGY+ ++ +    M  +K A   +     H D  +DPKA P ++GI   ++++ 
Sbjct: 364 KTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAE 423

Query: 431 NSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAK---WHCAFVSGFSTSVFPMPCPQL-- 477
           N + A  L GI    + G+C+ NVTI  LA  AK   W C  V G S+ V P PC  L  
Sbjct: 424 NVSMAARLEGIPSDPFTGICISNVTI-HLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPD 467

BLAST of Chy11G186210 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.8e-23
Identity = 115/451 (25.50%), Postives = 187/451 (41.46%), Query Frame = 0

Query: 57  SAFFRPDATAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVT 116
           S FF    T++  + +T FG VGDG T +++AF KA + +      G  +  VP G    
Sbjct: 11  SLFFLQIFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAV--CSGTGDGQFVVPAGMTF- 70

Query: 117 GSFNLTNEWPIIEPLPSYGRGRE-----RLGGRHISLIHGN------------ALSNVVI 176
                     +++PL   G  +      ++ G+ ++   GN             +  +VI
Sbjct: 71  ----------MLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVI 130

Query: 177 TGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCR 236
            G+ G I+GQG  WWE         +R   ++  + +N+ +S LT  +SP   IH   C 
Sbjct: 131 EGD-GEINGQGSSWWE------HKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECN 190

Query: 237 FHFQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCY 296
           +                         V I  + I AP ++PNTDGID  +S+NV I+DC 
Sbjct: 191 Y-------------------------VTISSLRINAPESSPNTDGIDVGASSNVVIQDCI 250

Query: 297 IESGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGG----ISNI 356
           I +GDD +A+ SG           + NI I  +        G+ IGS    G    + N+
Sbjct: 251 IATGDDCIAINSG-----------TSNIHISGID--CGPGHGISIGSLGKDGETATVENV 310

Query: 357 TIEDLNVWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAI---RFSRGSNDHPDEQF 416
            +++ N   +  G RIK+ QG  GY   ++     ++ V+  I   +F  G +    +  
Sbjct: 311 CVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDR 370

Query: 417 DPKAVPKVKGIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTI---------LGLAPS 474
              AV   K +F +N +  + ++    YG+     + V    + +          G    
Sbjct: 371 KSSAVEVSKVVF-SNFIGTSKSE----YGVDFRCSERVPCTEIFLRDMKIETASSGSGQV 398

BLAST of Chy11G186210 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 2.0e-22
Identity = 109/448 (24.33%), Postives = 199/448 (44.42%), Query Frame = 0

Query: 18  CSSLLLLFIFLAILTFQ--IATRTPPFPFWLPPSDFQ----------LPTCSAFFRPDAT 77
           C++L+ +F+  A+L F    A+R  P  +      F+          +    +F R    
Sbjct: 4   CTNLVTVFLLWALLMFSWCKASRISPNVYDHSYKRFKSDSLIKRREDITGLRSFVRASLR 63

Query: 78  AAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKG-SWVTGSFNLTNE 137
               +S++DFG  GDGKT +T+AF  A  + +     G   L VPKG +++  S  LT  
Sbjct: 64  TPTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGP 123

Query: 138 WPIIEPLPSYG---RGRERLGGRHIS-LIHGNALSNVVIT-GENGTIDGQGKMWWE--LW 197
              I  +  +G     ++R   + IS  I  + ++N+ +  G+ G +DG G+ WW+    
Sbjct: 124 CNSILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCK 183

Query: 198 WNRTLNHTRG-HLVELINSHNILISNLTFKNSPFWTIHPVYCRFHFQQLSTLSDYIHLCL 257
            N+    T+    +   NS ++++ NL  +N+              QQ+      I  C 
Sbjct: 184 RNKAKPCTKAPTALTFYNSKSLIVKNLKVRNA--------------QQIQI---SIEKC- 243

Query: 258 VLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQY 317
                  SNV + ++ + AP ++PNTDGI   ++ N+ + +  I +GDD ++++SG    
Sbjct: 244 -------SNVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG---- 303

Query: 318 GINLARPSLNIVIRRVSGTTPTCSGVGIGS----EMSGGISNITIEDLNVWDSAAGIRIK 377
                  S N+ I  +  T     G+ IGS         +S +T++   +  +  G+RIK
Sbjct: 304 -------SQNVQINDI--TCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIK 363

Query: 378 SDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLN 437
           + QG  G  +N+   N  M+ VK  I   +   D      +  AV +VK +   ++   +
Sbjct: 364 TYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAV-QVKNVVYRDISGTS 410

Query: 438 STKAPVLYGIA-GTSYDGVCMKNVTILG 440
           +++  + +  +      G+ +  V I G
Sbjct: 424 ASENAITFNCSKNYPCQGIVLDRVNIKG 410

BLAST of Chy11G186210 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.3e-18
Identity = 98/395 (24.81%), Postives = 166/395 (42.03%), Query Frame = 0

Query: 67  AVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKG-------SWVTGSF 126
           A  +S+++FG  GDGKT +T+AF+KA  + +     G +   VPKG       +   G  
Sbjct: 65  ASTVSVSNFGAKGDGKTDDTQAFKKA--WKKACSTNGVTTFLVPKGKTYLLKSTRFRGPC 124

Query: 127 NLTNEWPIIEPLPSYGRGRERLGGRHISLIHG-NALSNVVITGENGTIDGQGKMWWE--L 186
                + I+  L +  +  +     H  ++   N LS  +  G  G I+G GK WW+   
Sbjct: 125 KSLRNFQILGTLSASTKRSDYKDKNHWLILEDVNNLS--IDGGSTGIINGNGKTWWQNSC 184

Query: 187 WWNRTLNHTRG-HLVELINSHNILISNLTFKNSPFWTIHPVYCRFHFQQLSTLSDYIHLC 246
             +++   T+    + L N  N+ + NL  KN+              QQ+      I  C
Sbjct: 185 KIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNA--------------QQIQI---SIEKC 244

Query: 247 LVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQ 306
                   + V + ++ I AP ++PNTDGI   ++ N+ + +  I +GDD ++++ G   
Sbjct: 245 --------NKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQN 304

Query: 307 YGINLARPSLNIVIRRVSGTTPTCSGVGIGS----EMSGGISNITIEDLNVWDSAAGIRI 366
             I                T     G+ IGS         +S I ++     +S  G+RI
Sbjct: 305 LQI-------------FDLTCGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRI 364

Query: 367 KSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSL 426
           K+ QG  G   N+   N  M  VK  I   +   D  D+  D ++  +VK +   N+   
Sbjct: 365 KTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCD-KDKCEDQESAVQVKNVVYKNISGT 416

Query: 427 NSTKAPVLYGIAGT-SYDGVCMKNVTILGLAPSAK 446
           ++T   +    +      G+ ++NV I G   S K
Sbjct: 425 SATDVAITLNCSEKYPCQGIVLENVKIKGGTASCK 416

BLAST of Chy11G186210 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 5.5e-17
Identity = 94/411 (22.87%), Postives = 170/411 (41.36%), Query Frame = 0

Query: 48  PSDFQLPTCSAFFRPDATAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKL 107
           PS   L   +    P+ +    +S+ DFG  GDG T +T+AF KA     +     GS L
Sbjct: 63  PSILSLERFNPVGGPETSPDTDISVDDFGARGDG-TDDTKAFEKA----WKDACSSGSVL 122

Query: 108 TVPKGSWVTGSFNLTNEWPIIEPLPSYGRG--------RERLGGRHISLIHGNALSNVVI 167
            VP+         +T   P    L    RG         + +G      I    +SN+ +
Sbjct: 123 IVPENKNYLLK-QITFSGPCKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTL 182

Query: 168 TGENGTIDGQGKMWWELWWNRTLN---HTRGHLVELINSHNILISNLTFKNSPFWTIHPV 227
            G  GT +G G+ WW+    R  +    +    +   +  N+++S+L+ K+S    +   
Sbjct: 183 EG-GGTSNGNGETWWDSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFD 242

Query: 228 YCRFHFQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIE 287
            C+                         +V+  ++ + AP ++PNTDGI    +  + + 
Sbjct: 243 KCQ-------------------------DVIASNLMVTAPEHSPNTDGIHITGTQRIHVM 302

Query: 288 DCYIESGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCS---GVGIGS----EMS 347
           +  I +GDD ++++SG                 + V  T  TC    G+ IGS       
Sbjct: 303 NSVIGTGDDCISIESG----------------SKMVIATNITCGPGHGISIGSLGDRNSE 362

Query: 348 GGISNITIEDLNVWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPD 407
             +S + ++  N++D+  G+RIK+ QG  G   N+   N VM+ V   I   +   D  D
Sbjct: 363 AHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKD 422

Query: 408 EQFDPKAVPKVKGIFITNLMSLNSTKAPVLYGIAGTS-YDGVCMKNVTILG 440
              + ++  KV  +   N+   ++++  V +  + +S   G  + N+ ++G
Sbjct: 423 PCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVG 425

BLAST of Chy11G186210 vs. ExPASy TrEMBL
Match: A0A0A0LJW9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G334510 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 5.7e-259
Identity = 448/497 (90.14%), Postives = 449/497 (90.34%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60
           MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60

Query: 61  RPDATAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120
           RPDATA VVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN
Sbjct: 61  RPDATAGVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120

Query: 121 LT-------------------NEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180
           LT                   +EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG
Sbjct: 121 LTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180

Query: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFH 240
           ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC   
Sbjct: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC--- 240

Query: 241 FQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300
                                 SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE
Sbjct: 241 ----------------------SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300

Query: 301 SGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360
           SGDDLVAVKSGWDQYGINLARPS NIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN
Sbjct: 301 SGDDLVAVKSGWDQYGINLARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360

Query: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420
           VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK
Sbjct: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420

Query: 421 GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 479
           GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGL PSAKWHCAFVSGFSTSVFP 
Sbjct: 421 GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLTPSAKWHCAFVSGFSTSVFPT 472

BLAST of Chy11G186210 vs. ExPASy TrEMBL
Match: A0A5A7TGP3 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold703G00210 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.2e-258
Identity = 448/497 (90.14%), Postives = 449/497 (90.34%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60
           MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60

Query: 61  RPDATAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120
           RPD TAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN
Sbjct: 61  RPDVTAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120

Query: 121 LT-------------------NEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180
           LT                   +EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG
Sbjct: 121 LTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180

Query: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFH 240
           ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC   
Sbjct: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC--- 240

Query: 241 FQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300
                                 SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE
Sbjct: 241 ----------------------SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300

Query: 301 SGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360
           SGDDLVAVKSGWDQYGINLARPS NIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN
Sbjct: 301 SGDDLVAVKSGWDQYGINLARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360

Query: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420
           VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK
Sbjct: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420

Query: 421 GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 479
           GIFITNLMSLNSTKAPVL GIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM
Sbjct: 421 GIFITNLMSLNSTKAPVLDGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 472

BLAST of Chy11G186210 vs. ExPASy TrEMBL
Match: A0A1S3CDX7 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103499630 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.2e-258
Identity = 448/497 (90.14%), Postives = 449/497 (90.34%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60
           MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF
Sbjct: 42  MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 101

Query: 61  RPDATAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120
           RPD TAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN
Sbjct: 102 RPDVTAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 161

Query: 121 LT-------------------NEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180
           LT                   +EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG
Sbjct: 162 LTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 221

Query: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFH 240
           ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC   
Sbjct: 222 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC--- 281

Query: 241 FQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300
                                 SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE
Sbjct: 282 ----------------------SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 341

Query: 301 SGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360
           SGDDLVAVKSGWDQYGINLARPS NIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN
Sbjct: 342 SGDDLVAVKSGWDQYGINLARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 401

Query: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420
           VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK
Sbjct: 402 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 461

Query: 421 GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 479
           GIFITNLMSLNSTKAPVL GIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM
Sbjct: 462 GIFITNLMSLNSTKAPVLDGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 513

BLAST of Chy11G186210 vs. ExPASy TrEMBL
Match: A0A6J1G526 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111450990 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.7e-224
Identity = 394/501 (78.64%), Postives = 420/501 (83.83%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQL-----PT 60
           MD +KS   FG  INPSCSSLLLLFIF+AILT QIATRT PFP W+P S         PT
Sbjct: 1   MDGEKSS--FGSLINPSCSSLLLLFIFIAILTLQIATRT-PFPIWMPSSTLTTDTDSEPT 60

Query: 61  CSAFFRPDAT-AAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSW 120
           CSAFF  DA    VVMSITDFGGVGDG+TSNTEAFRKAI+YMRRFGDKGGSKL VPKG W
Sbjct: 61  CSAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRW 120

Query: 121 VTGSFNLT-------------------NEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           VTGSFNLT                   +EWPIIEPLPSYGRGRERLGGRHISLIHGNALS
Sbjct: 121 VTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180

Query: 181 NVVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHP 240
           NV+ITGENGTIDGQGKMWW+LWWNRTLNHTRGHL+ELINSHNILISNLT KNSPFWT+HP
Sbjct: 181 NVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHP 240

Query: 241 VYCRFHFQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCI 300
           VYC                         SNVVIK+MTILAPLNAPNTDGIDPDSSTN+CI
Sbjct: 241 VYC-------------------------SNVVIKNMTILAPLNAPNTDGIDPDSSTNICI 300

Query: 301 EDCYIESGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNI 360
           EDCYIESGDDLVAVKSGWDQYGINLARPS NI+IRRVSGTTPTCSGVGIGSEMSGGISNI
Sbjct: 301 EDCYIESGDDLVAVKSGWDQYGINLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNI 360

Query: 361 TIEDLNVWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPK 420
           TIEDLN+WDSAAG+RIKSDQGRGGYIAN+SI+NFVMNRVKMAIRFS+GSNDHPDE++DPK
Sbjct: 361 TIEDLNIWDSAAGVRIKSDQGRGGYIANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPK 420

Query: 421 AVPKVKGIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFS 477
           AVP VKGIFI+N+ SLNSTKAPVL GI GT +DGVCMKNVTILGLAPSAKWHCAFVSGFS
Sbjct: 421 AVPMVKGIFISNITSLNSTKAPVLNGIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFS 473

BLAST of Chy11G186210 vs. ExPASy TrEMBL
Match: A0A6J1L474 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111499740 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.5e-222
Identity = 390/502 (77.69%), Postives = 419/502 (83.47%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQL------P 60
           MD +KS   F P INPSCSSLLLLFIF+AILT QIATRT PFP W+P S          P
Sbjct: 1   MDGEKSS--FAPLINPSCSSLLLLFIFIAILTLQIATRT-PFPIWMPSSTLATTDTDSEP 60

Query: 61  TCSAFFRPDATA-AVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGS 120
           TCSAFF  DA    VVM ITDFGGVGDG+TSNTEAFRKAI+YMRRFG+ GGSKL VPKGS
Sbjct: 61  TCSAFFHRDAAPDTVVMLITDFGGVGDGRTSNTEAFRKAIEYMRRFGETGGSKLIVPKGS 120

Query: 121 WVTGSFNLT-------------------NEWPIIEPLPSYGRGRERLGGRHISLIHGNAL 180
           W+TGSFNLT                   +EWPIIEPLPSYGRGRERLGGRHISLIHGNAL
Sbjct: 121 WITGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNAL 180

Query: 181 SNVVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIH 240
           SNV+ITGENGTIDGQGKMWW+LWWNRTLNHTRGHL+ELINSHNILISNLT KNSPFWT+H
Sbjct: 181 SNVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVH 240

Query: 241 PVYCRFHFQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVC 300
           PVYC                         SNVVIK+MTILAPLNAPNTDGIDPDSSTN+C
Sbjct: 241 PVYC-------------------------SNVVIKNMTILAPLNAPNTDGIDPDSSTNIC 300

Query: 301 IEDCYIESGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISN 360
           IEDCYIESGDDLVAVKSGWDQYGINLARPS NI+IRRVSGTTPTCSGVGIGSEMSGGISN
Sbjct: 301 IEDCYIESGDDLVAVKSGWDQYGINLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISN 360

Query: 361 ITIEDLNVWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDP 420
           ITI+DLN+WDSAAG+RIKSDQGRGGYIAN+SI+NFVMNRVKMAIRFS+GSNDHPDE++DP
Sbjct: 361 ITIDDLNIWDSAAGVRIKSDQGRGGYIANISISNFVMNRVKMAIRFSKGSNDHPDEEWDP 420

Query: 421 KAVPKVKGIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGF 477
           KAVP VKGIFI+N+ SLNSTKAPVL GI GT +DGVCMKNVTILGLAPSAKWHCAFVSGF
Sbjct: 421 KAVPIVKGIFISNITSLNSTKAPVLNGIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGF 474

BLAST of Chy11G186210 vs. NCBI nr
Match: XP_004147365.1 (probable polygalacturonase [Cucumis sativus] >KGN62200.1 hypothetical protein Csa_016932 [Cucumis sativus])

HSP 1 Score: 900 bits (2327), Expect = 0.0
Identity = 448/497 (90.14%), Postives = 449/497 (90.34%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60
           MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60

Query: 61  RPDATAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120
           RPDATA VVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN
Sbjct: 61  RPDATAGVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120

Query: 121 LTN-------------------EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180
           LT+                   EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG
Sbjct: 121 LTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180

Query: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFH 240
           ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC   
Sbjct: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC--- 240

Query: 241 FQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300
                                 SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE
Sbjct: 241 ----------------------SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300

Query: 301 SGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360
           SGDDLVAVKSGWDQYGINLARPS NIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN
Sbjct: 301 SGDDLVAVKSGWDQYGINLARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360

Query: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420
           VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK
Sbjct: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420

Query: 421 GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 478
           GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGL PSAKWHCAFVSGFSTSVFP 
Sbjct: 421 GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLTPSAKWHCAFVSGFSTSVFPT 472

BLAST of Chy11G186210 vs. NCBI nr
Match: KAA0040757.1 (putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 448/497 (90.14%), Postives = 449/497 (90.34%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60
           MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60

Query: 61  RPDATAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120
           RPD TAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN
Sbjct: 61  RPDVTAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120

Query: 121 LTN-------------------EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180
           LT+                   EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG
Sbjct: 121 LTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180

Query: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFH 240
           ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC   
Sbjct: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC--- 240

Query: 241 FQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300
                                 SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE
Sbjct: 241 ----------------------SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300

Query: 301 SGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360
           SGDDLVAVKSGWDQYGINLARPS NIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN
Sbjct: 301 SGDDLVAVKSGWDQYGINLARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360

Query: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420
           VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK
Sbjct: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420

Query: 421 GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 478
           GIFITNLMSLNSTKAPVL GIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM
Sbjct: 421 GIFITNLMSLNSTKAPVLDGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 472

BLAST of Chy11G186210 vs. NCBI nr
Match: XP_008460889.1 (PREDICTED: probable polygalacturonase [Cucumis melo])

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 448/497 (90.14%), Postives = 449/497 (90.34%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60
           MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF
Sbjct: 42  MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 101

Query: 61  RPDATAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 120
           RPD TAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN
Sbjct: 102 RPDVTAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFN 161

Query: 121 LTN-------------------EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 180
           LT+                   EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG
Sbjct: 162 LTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITG 221

Query: 181 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFH 240
           ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC   
Sbjct: 222 ENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC--- 281

Query: 241 FQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 300
                                 SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE
Sbjct: 282 ----------------------SNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIE 341

Query: 301 SGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 360
           SGDDLVAVKSGWDQYGINLARPS NIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN
Sbjct: 342 SGDDLVAVKSGWDQYGINLARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN 401

Query: 361 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 420
           VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK
Sbjct: 402 VWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVK 461

Query: 421 GIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 478
           GIFITNLMSLNSTKAPVL GIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM
Sbjct: 462 GIFITNLMSLNSTKAPVLDGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPM 513

BLAST of Chy11G186210 vs. NCBI nr
Match: XP_038900759.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 838 bits (2165), Expect = 1.21e-304
Identity = 424/498 (85.14%), Postives = 436/498 (87.55%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFF 60
           MD +KSKF FGPPINPSCSSLLLLFIFLAILTFQIATRTP    WLP SDFQ PTCSAFF
Sbjct: 1   MDGEKSKFSFGPPINPSCSSLLLLFIFLAILTFQIATRTPFPTIWLP-SDFQQPTCSAFF 60

Query: 61  RPDA---TAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTG 120
           R DA   TAAVVMSITDFGGVGDGKTSNTEAFRKAI+YMRRFG+KGGSKLTVPKGSWVTG
Sbjct: 61  RRDAAADTAAVVMSITDFGGVGDGKTSNTEAFRKAIEYMRRFGEKGGSKLTVPKGSWVTG 120

Query: 121 SFNLTN-------------------EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVV 180
           SFNLT+                   EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVV
Sbjct: 121 SFNLTSNFTLFLQRGALILASQDPEEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVV 180

Query: 181 ITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYC 240
           ITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELI+SHNILISNLT KNSPFWTIHPVYC
Sbjct: 181 ITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELIDSHNILISNLTLKNSPFWTIHPVYC 240

Query: 241 RFHFQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDC 300
                                    SNVVIKDMTILAPLNAPNTDGIDPDSS NVCIEDC
Sbjct: 241 -------------------------SNVVIKDMTILAPLNAPNTDGIDPDSSINVCIEDC 300

Query: 301 YIESGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIE 360
           YIESGDDLVAVKSGWDQYG+NLARPS NI+IRRVSGTTPTCSGVGIGSEMSGGISNITIE
Sbjct: 301 YIESGDDLVAVKSGWDQYGMNLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIE 360

Query: 361 DLNVWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVP 420
           DLN+WDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFS+GSNDHPDEQ+DPKAVP
Sbjct: 361 DLNIWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSKGSNDHPDEQWDPKAVP 420

Query: 421 KVKGIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSV 476
           KVKGIFITNL+SLNSTKAPVL GI GTSYDGVCMKNVTILGLAPSAKWHCAF+SGFSTSV
Sbjct: 421 KVKGIFITNLISLNSTKAPVLDGIKGTSYDGVCMKNVTILGLAPSAKWHCAFISGFSTSV 472

BLAST of Chy11G186210 vs. NCBI nr
Match: XP_022946971.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 785 bits (2027), Expect = 1.31e-283
Identity = 394/501 (78.64%), Postives = 420/501 (83.83%), Query Frame = 0

Query: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQL-----PT 60
           MD +KS F  G  INPSCSSLLLLFIF+AILT QIATRTP FP W+P S         PT
Sbjct: 1   MDGEKSSF--GSLINPSCSSLLLLFIFIAILTLQIATRTP-FPIWMPSSTLTTDTDSEPT 60

Query: 61  CSAFFRPDATA-AVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSW 120
           CSAFF  DA    VVMSITDFGGVGDG+TSNTEAFRKAI+YMRRFGDKGGSKL VPKG W
Sbjct: 61  CSAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRW 120

Query: 121 VTGSFNLT-------------------NEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           VTGSFNLT                   +EWPIIEPLPSYGRGRERLGGRHISLIHGNALS
Sbjct: 121 VTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180

Query: 181 NVVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHP 240
           NV+ITGENGTIDGQGKMWW+LWWNRTLNHTRGHL+ELINSHNILISNLT KNSPFWT+HP
Sbjct: 181 NVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHP 240

Query: 241 VYCRFHFQQLSTLSDYIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCI 300
           VYC                         SNVVIK+MTILAPLNAPNTDGIDPDSSTN+CI
Sbjct: 241 VYC-------------------------SNVVIKNMTILAPLNAPNTDGIDPDSSTNICI 300

Query: 301 EDCYIESGDDLVAVKSGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNI 360
           EDCYIESGDDLVAVKSGWDQYGINLARPS NI+IRRVSGTTPTCSGVGIGSEMSGGISNI
Sbjct: 301 EDCYIESGDDLVAVKSGWDQYGINLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNI 360

Query: 361 TIEDLNVWDSAAGIRIKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPK 420
           TIEDLN+WDSAAG+RIKSDQGRGGYIAN+SI+NFVMNRVKMAIRFS+GSNDHPDE++DPK
Sbjct: 361 TIEDLNIWDSAAGVRIKSDQGRGGYIANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPK 420

Query: 421 AVPKVKGIFITNLMSLNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFS 476
           AVP VKGIFI+N+ SLNSTKAPVL GI GT +DGVCMKNVTILGLAPSAKWHCAFVSGFS
Sbjct: 421 AVPMVKGIFISNITSLNSTKAPVLNGIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFS 473

BLAST of Chy11G186210 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 622.5 bits (1604), Expect = 2.9e-178
Identity = 305/472 (64.62%), Postives = 369/472 (78.18%), Query Frame = 0

Query: 16  PSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLP-TCSAFFRPDA-TAAVVMSIT 75
           PS + LLL+F  LAIL+ QI++ +   P W+P S +  P TCS FF  D     +VMSIT
Sbjct: 17  PSWAFLLLVFTVLAILSLQISSNS-FLPLWIPTSQYDDPVTCSGFFNHDPFPNRIVMSIT 76

Query: 76  DFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFNLT----------- 135
           DFGGVGDGKTSNT AFR+A++++  F  +GG++L VP+G+W++GSFNLT           
Sbjct: 77  DFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTLFLERGA 136

Query: 136 --------NEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGENGTIDGQGKMWW 195
                   +EWPIIEPLPSYGRGRER GGRHISLIHG+ L+NVVITGENGTIDGQGKMWW
Sbjct: 137 LILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDGQGKMWW 196

Query: 196 ELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFHFQQLSTLSDYIHL 255
           ELWWNRTL HTRGHL+EL NSHNILISNLT  NSPFWTIHPVYC                
Sbjct: 197 ELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYC---------------- 256

Query: 256 CLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD 315
                    SNVVI++MTILAP+NAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD
Sbjct: 257 ---------SNVVIRNMTILAPMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWD 316

Query: 316 QYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSD 375
           QYG+ +ARPS NIVIRR+SGTT TCSGVGIGSEMSGGI NIT+ED++VWDSAAG+RIK+D
Sbjct: 317 QYGMAVARPSSNIVIRRISGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTD 376

Query: 376 QGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNST 435
           +GRGGYI+N++  N ++ +VK+ IRFS GSNDH D+++DPKA+P+VKGI+I+N++SLNS 
Sbjct: 377 KGRGGYISNITFNNVLLEKVKVPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSR 436

Query: 436 KAPVLYGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQL 467
           KAP+L G+ GTS+  VC++NVT+LGL  + KW C  VSG+++ VFP+ CPQL
Sbjct: 437 KAPMLLGVEGTSFQDVCLRNVTLLGLPKTEKWKCKDVSGYASDVFPLSCPQL 462

BLAST of Chy11G186210 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 426.4 bits (1095), Expect = 3.0e-119
Identity = 217/424 (51.18%), Postives = 282/424 (66.51%), Query Frame = 0

Query: 70  MSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFNL-------- 129
           +SIT+FG VGDG T NT+AF+ A+ Y+  F DKGG+KL VP G W+TGSF+L        
Sbjct: 43  VSITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWL 102

Query: 130 -----------TNEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGENGTIDGQG 189
                      +  WP+++PLPSYGRGRE  G RH SLI+G  L++VVITGENGTIDGQG
Sbjct: 103 DKGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQG 162

Query: 190 KMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFHFQQLSTLSD 249
            +WW+ + N  LN+TR HLVEL+NS  ++ISNLTF NSPFW IHPVYCR           
Sbjct: 163 TVWWDWFRNGELNYTRPHLVELMNSTGLIISNLTFLNSPFWNIHPVYCR----------- 222

Query: 250 YIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVK 309
                         +VV+K++TILAPL +PNTDG+DPDSSTNVCIEDCYI +GDDLV++K
Sbjct: 223 --------------DVVVKNLTILAPLESPNTDGVDPDSSTNVCIEDCYIVTGDDLVSIK 282

Query: 310 SGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIR 369
           SGWD+YGI+ ARPS  I I R++G T + SG+ IGSEMSGG+S I I+DL++++S  GIR
Sbjct: 283 SGWDEYGISYARPSSKIKINRLTGQTTSSSGIAIGSEMSGGVSEIYIKDLHLFNSNTGIR 342

Query: 370 IKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMS 429
           IK+  GRGGY+ NV I N  ++ VK AIRF+    +HPDE++DPKA+P ++ I   N+  
Sbjct: 343 IKTSAGRGGYVRNVHILNVKLDNVKKAIRFTGKYGEHPDEKYDPKALPAIEKITFENVNG 402

Query: 430 LNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAK--WHCAFVSGFSTSVFP-MPCPQLQ 472
                A +L GI G  +  +C  NVT+     S K  W C+ V G+S  V P + C  L+
Sbjct: 403 DGIGVAGLLEGIEGDVFKNICFLNVTLRVKKNSKKSPWECSNVRGYSQWVSPEITCDSLK 441

BLAST of Chy11G186210 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 419.1 bits (1076), Expect = 4.8e-117
Identity = 210/428 (49.07%), Postives = 281/428 (65.65%), Query Frame = 0

Query: 70  MSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFNLTN------ 129
           +SITDFGGVGDG+T NT+AFR AI  ++    +GG+ L +P G ++T SFNLT+      
Sbjct: 41  ISITDFGGVGDGRTVNTKAFRAAIYRIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYL 100

Query: 130 -------------EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGENGTIDGQG 189
                         WP+I+PLPSYGRGRE  GGR++S IHG+ L +VVITG+NGTIDGQG
Sbjct: 101 AKGAVIRAVQDTWNWPLIDPLPSYGRGRELPGGRYMSFIHGDGLRDVVITGQNGTIDGQG 160

Query: 190 KMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFHFQQLSTLSD 249
           ++WW +W +RTL +TR +L+E  +S  I+ISN+ F+NSPFW IHPVYC            
Sbjct: 161 EVWWNMWRSRTLKYTRPNLIEFKDSKEIIISNVIFQNSPFWNIHPVYC------------ 220

Query: 250 YIHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVK 309
                        SNVVI  +TILAP ++PNTDGIDPDSS NVCIED YI +GDDLVA+K
Sbjct: 221 -------------SNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYISTGDDLVAIK 280

Query: 310 SGWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIR 369
           SGWDQYGI   RPS NI IRR++G++P  +G+ IGSE SGGI NI  E + + +   G+ 
Sbjct: 281 SGWDQYGIAYGRPSSNITIRRITGSSP-FAGIAIGSETSGGIKNIIAEHITLSNMGVGVN 340

Query: 370 IKSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMS 429
           IK++ GRGGYI N+ I++  ++  K  I+ +  + DHPDE ++P A+P VKGI I N+  
Sbjct: 341 IKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVVKGIHIKNVWG 400

Query: 430 LNSTKAPVLYGIAGTSYDGVCMKNVTILGLAPSAK-WHCAFVSGFSTSVFPMPCPQLQNT 478
           +N   A  + G+ G+ + G+C+  + + G   S K W C+ VSG S  V P PC +L+ T
Sbjct: 401 VNVRNAGSIQGLKGSPFTGICLSEINLHGSLNSYKTWKCSDVSGTSLKVSPWPCSELRTT 442

BLAST of Chy11G186210 vs. TAIR 10
Match: AT5G41870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 391.0 bits (1003), Expect = 1.4e-108
Identity = 206/471 (43.74%), Postives = 288/471 (61.15%), Query Frame = 0

Query: 20  SLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQLPTCSAFFRPDATAAVVMSITDFGGVG 79
           S+ +LF+   +L   ++   P              TCS    P      ++SI+DFG VG
Sbjct: 9   SVFVLFLLSTVLPHHLSLGAP-------------ITCSGIV-PMKHRNEMLSISDFGAVG 68

Query: 80  DGKTSNTEAFRKAIQYMRRFGDKG-GSKLTVPKGSWVTGSFNLTN--------------- 139
           DGKT NT+AF  AI  +R   +   G+ L VP+G ++T SFNLT+               
Sbjct: 69  DGKTLNTKAFNSAIDRIRNSNNSNEGTLLYVPRGVYLTQSFNLTSHMTLYLADGAVIKAV 128

Query: 140 ----EWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGENGTIDGQGKMWWELWWN 199
               +WP+ +PLPSYGRGRE  G R+IS IHG+ L++VVITG NGTIDGQG+ WW +W +
Sbjct: 129 QDTEKWPLTDPLPSYGRGREHPGRRYISFIHGDGLNDVVITGRNGTIDGQGEPWWNMWRH 188

Query: 200 RTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFHFQQLSTLSDYIHLCLVLE 259
            TL  TR  L+E  NS NIL+S++  +NSPFWT+HPVYC                     
Sbjct: 189 GTLKFTRPGLIEFNNSTNILVSHVVLQNSPFWTLHPVYC--------------------- 248

Query: 260 LLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGIN 319
               SNVV+  +TILAP ++ NTDGIDPDSS+NVCIED YI +GDDLVAVKSGWD+YGI 
Sbjct: 249 ----SNVVVHHVTILAPTDSYNTDGIDPDSSSNVCIEDSYISTGDDLVAVKSGWDEYGIA 308

Query: 320 LARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGG 379
             RPS +I IRR++G++P  +G+ IGSE SGGI N+T+E++ +++S  GI IK++ GRGG
Sbjct: 309 YNRPSRDITIRRITGSSP-FAGIAIGSETSGGIQNVTVENITLYNSGIGIHIKTNIGRGG 368

Query: 380 YIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVL 439
            I  ++I+   + +V+  I+ S  + DHPD++F+  A+P V+GI I N+  +   +A ++
Sbjct: 369 SIQGITISGVYLEKVRTGIKISGDTGDHPDDKFNTSALPIVRGITIKNVWGIKVERAGMV 428

Query: 440 YGIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQLQNTT 471
            G+  + +  +C  NVT+ G   S  W C+ V G +  V P PCP+L  TT
Sbjct: 429 QGLKDSPFTNLCFSNVTLTGTKRSPIWKCSDVVGAADKVNPTPCPELSATT 439

BLAST of Chy11G186210 vs. TAIR 10
Match: AT4G23500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 370.2 bits (949), Expect = 2.6e-102
Identity = 192/417 (46.04%), Postives = 258/417 (61.87%), Query Frame = 0

Query: 71  SITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWVTGSFNLT-------- 130
           S+ +FG VGDG TSNT AFR A+  + RF D GGS L VP G W+TG+FNLT        
Sbjct: 69  SLDEFGAVGDGVTSNTAAFRDAVSQLSRFADYGGSLLFVPAGRWLTGNFNLTSHFTLFLH 128

Query: 131 -----------NEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGENGTIDGQGK 190
                      +++ +IEPLPSYGRGR+  GGR ISL+ G+ L++VVITGENGTIDGQG+
Sbjct: 129 RDAVILASQEESDYEVIEPLPSYGRGRDTDGGRFISLLFGSNLTDVVITGENGTIDGQGE 188

Query: 191 MWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPVYCRFHFQQLSTLSDY 250
            WW  +    L +TR +L+E+++S  I ISNLTF NSP W IHPVY              
Sbjct: 189 PWWGKFKRGELKYTRPYLIEIMHSDGIQISNLTFLNSPSWHIHPVYS------------- 248

Query: 251 IHLCLVLELLFFSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKS 310
                       SN+ I+ +TILAP+  PNTDGI+PDS TN  IEDCYI SGDD +AVKS
Sbjct: 249 ------------SNIYIQGLTILAPVTVPNTDGINPDSCTNTRIEDCYIVSGDDCIAVKS 308

Query: 311 GWDQYGINLARPSLNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRI 370
           GWDQYGIN   P+  ++IRR++  +P  + + +GSEMSGGI ++  ED+   +S +GIRI
Sbjct: 309 GWDQYGINYGMPTKQLLIRRLTCISPDSAVIALGSEMSGGIEDVRAEDIVAINSESGIRI 368

Query: 371 KSDQGRGGYIANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSL 430
           K+  GRGGY+ +V +    M  +K     +     HPD+ +DPKA+P ++ I   ++++ 
Sbjct: 369 KTAIGRGGYVKDVYVRGMTMMTMKYVFWMTGSYGSHPDDHYDPKALPVIQNINYQDMVAE 428

Query: 431 NSTKAPVLYGIAGTSYDGVCMKNVTI-LGLAP-SAKWHCAFVSGFSTSVFPMPCPQL 467
           N T    L GI+G  + G+C+ NVTI L   P    W+C  VSG+++ V P PC  L
Sbjct: 429 NVTMPAQLAGISGDQFTGICISNVTITLSKKPKKVLWNCTDVSGYTSGVTPQPCQLL 460

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL31.1e-10044.42Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q9LW071.8e-2325.50Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q8RY292.0e-2224.33Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
O231471.3e-1824.81Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
Q020965.5e-1722.87Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LJW95.7e-25990.14Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G334510 PE=3 SV=1[more]
A0A5A7TGP32.2e-25890.14Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3CDX72.2e-25890.14probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103499630 PE=3 SV=1[more]
A0A6J1G5262.7e-22478.64probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111450990 PE=3 SV... [more]
A0A6J1L4742.5e-22277.69probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111499740 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147365.10.090.14probable polygalacturonase [Cucumis sativus] >KGN62200.1 hypothetical protein Cs... [more]
KAA0040757.10.090.14putative polygalacturonase [Cucumis melo var. makuwa][more]
XP_008460889.10.090.14PREDICTED: probable polygalacturonase [Cucumis melo][more]
XP_038900759.11.21e-30485.14probable polygalacturonase [Benincasa hispida][more]
XP_022946971.11.31e-28378.64probable polygalacturonase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G33440.12.9e-17864.62Pectin lyase-like superfamily protein [more]
AT3G62110.13.0e-11951.18Pectin lyase-like superfamily protein [more]
AT4G23820.14.8e-11749.07Pectin lyase-like superfamily protein [more]
AT5G41870.11.4e-10843.74Pectin lyase-like superfamily protein [more]
AT4G23500.12.6e-10246.04Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 195..220
e-value: 6600.0
score: 1.4
coord: 270..302
e-value: 340.0
score: 10.0
coord: 332..353
e-value: 27.0
score: 13.7
coord: 243..269
e-value: 4700.0
score: 2.4
coord: 304..326
e-value: 5400.0
score: 2.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 55..452
e-value: 1.4E-94
score: 319.3
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 244..451
e-value: 4.8E-27
score: 94.9
coord: 147..214
e-value: 5.6E-7
score: 28.9
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 13..220
NoneNo IPR availablePANTHERPTHR31339:SF4PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 13..220
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 241..476
NoneNo IPR availablePANTHERPTHR31339:SF4PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 241..476
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 64..468

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G186210.1Chy11G186210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004650 polygalacturonase activity