Chy10G183520 (gene) Cucumber (hystrix) v1

Overview
NameChy10G183520
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAspergillus nuclease S(1)
LocationchrH10: 16463287 .. 16464962 (+)
RNA-Seq ExpressionChy10G183520
SyntenyChy10G183520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCTTGTAGATTTCTGATAGTGGCCATTTTGTCGTTGGTGTTCATCTTTCCTGTTAGCTTTGGTTGGGGAATTGACGGCCATTTTACTGTCTGCAAAATCGCTCAGGTTCTTCTCTTCACATTCTTTCACAAACCCTTTTCTCAAGTTTACCTTTTTTCATGAAAAATTTTGATCTTTGTTTTGTGTTGCTAAAAGTCACGGTTGAGCAAAGCAGCAGCAGATGCAGTACAAGAACTGTTGCCAGAGTCCGCACAGGGAGATTTAGCAAGCGTTTGCATTTGGGCAGATAGAGTTAAGTTTCGTTATCGTTGGTCCCCTCCTCTTCATTTCATTGATACTCCAGATTCCCTTTGTACTTACCAGTATGACAGTTGAGTTACCTCTTTTATCTCCATTCTTCTCTGTAGTCTGTTCTAATTTCTCCGAGCCTTCTTCTCTTATGGTTTTAAAAATCTTGTCATTTGAATCTCTTAGGAGACTGCAAAGATGAAGCTGGAGAGAAAGGACGGTGTGTTGCTGGCGCCATTAACAACTATACTTCCCAGCTTCTAACCTACAATGCTCAACCTTCTAACTCTGAATGTAATATTCAAGAAAACGAGAATCTTAAATACTTTGCTTTCAATGAGAAACCGATGAAATTGCTAATACGCTATGACTTTTTCATCATCAGATAACCTGACCGAAGCATTGCTTTTTCTTTCACATTTCATGGGAGACATCCATCAGGTATGATATGAAGTTTATGTACATGTTGTGTGCAATTACTTACTGAAATGAACTTACGTAAAATGCAGCCTCTGCACGTGGGCTTCACGGGAGACAGGGGAGGAAACACAATCGACGTTCATTGGTACACCAGGAAGCAAAATCTTCACCATGTAAATATAATTACCCAGCTTTATCTCTATATATTGGAAGTTTCCAGTTTTGAATCAGTGAAAATACTGTATTTCTCGTGATTCTAACAGATATGGGATAGCAACATAATCGAAACAGCAGAGGGCAAATTCTACGACTTCAGTGTGGATGGCCTAGTTGATGCAATCCAAACGAATATAACGGTTACATAATTGATAATTCTATGTCCAAATCAATTAGAGAACACAAAGCCTGATTCTTATCAATACTAAGCAATTCTAATGCATTTTTGTCCCCTTTTGGTGTCCTGATCAACAGAATGAATGGGCAGATCAAGCCTGATTCTTATCAATACTAAGCAATTCTAATGCATTTTTGTCACCTTTTGGTGTCCTGATCAACAGAATGAATGGGCAGATCAAGTTGAAGAATGGGAAAAGTGTGGTTCTGATGAAGTACCATGCACAGAAATGTATGTGGCACAAGAAACAGTCTGTTAATCCTTCAATTATAGAACATGAACTTCCAATAATTTACCATCAGTTTTCATTGAAATATTCAGATATGCATCAGAAAGTATTCAGGCAGCCTGTGACTGGGCATACAAAGGCGTTTCTGAAGGTTCTACACTAGCAGGTAGATTTCTTACAAAACTTGGTTCAGTTGTTCTTTGAGCAGAAGAGTTTTTAACTTGAAAAAATTGCGATGCAGAGAAATATTTTGCCTCCCGTGTGCCAGTGTTGAACCTACGGTTGGCACAAGGTGGAGTCAGATTAGCAGCTGCTTTAAATCGCATCTTTGGTTCATAG

mRNA sequence

ATGGAGTCTTGTAGATTTCTGATAGTGGCCATTTTGTCGTTGGTGTTCATCTTTCCTGTTAGCTTTGGTTGGGGAATTGACGGCCATTTTACTGTCTGCAAAATCGCTCAGTCACGGTTGAGCAAAGCAGCAGCAGATGCAGTACAAGAACTGTTGCCAGAGTCCGCACAGGGAGATTTAGCAAGCGTTTGCATTTGGGCAGATAGAGTTAAGTTTCGTTATCGTTGGTCCCCTCCTCTTCATTTCATTGATACTCCAGATTCCCTTTGTACTTACCAGTATGACAGAGACTGCAAAGATGAAGCTGGAGAGAAAGGACGGTGTGTTGCTGGCGCCATTAACAACTATACTTCCCAGCTTCTAACCTACAATGCTCAACCTTCTAACTCTGAATATAACCTGACCGAAGCATTGCTTTTTCTTTCACATTTCATGGGAGACATCCATCAGCCTCTGCACGTGGGCTTCACGGGAGACAGGGGAGGAAACACAATCGACGTTCATTGGTACACCAGGAAGCAAAATCTTCACCATATATGGGATAGCAACATAATCGAAACAGCAGAGGGCAAATTCTACGACTTCAGTGTGGATGGCCTAGTTGATGCAATCCAAACGAATATAACGAATGAATGGGCAGATCAAGTTGAAGAATGGGAAAAGTGTGGTTCTGATGAAGTACCATGCACAGAAATATATGCATCAGAAAGTATTCAGGCAGCCTGTGACTGGGCATACAAAGGCGTTTCTGAAGGTTCTACACTAGCAGAGAAATATTTTGCCTCCCGTGTGCCAGTGTTGAACCTACGGTTGGCACAAGGTGGAGTCAGATTAGCAGCTGCTTTAAATCGCATCTTTGGTTCATAG

Coding sequence (CDS)

ATGGAGTCTTGTAGATTTCTGATAGTGGCCATTTTGTCGTTGGTGTTCATCTTTCCTGTTAGCTTTGGTTGGGGAATTGACGGCCATTTTACTGTCTGCAAAATCGCTCAGTCACGGTTGAGCAAAGCAGCAGCAGATGCAGTACAAGAACTGTTGCCAGAGTCCGCACAGGGAGATTTAGCAAGCGTTTGCATTTGGGCAGATAGAGTTAAGTTTCGTTATCGTTGGTCCCCTCCTCTTCATTTCATTGATACTCCAGATTCCCTTTGTACTTACCAGTATGACAGAGACTGCAAAGATGAAGCTGGAGAGAAAGGACGGTGTGTTGCTGGCGCCATTAACAACTATACTTCCCAGCTTCTAACCTACAATGCTCAACCTTCTAACTCTGAATATAACCTGACCGAAGCATTGCTTTTTCTTTCACATTTCATGGGAGACATCCATCAGCCTCTGCACGTGGGCTTCACGGGAGACAGGGGAGGAAACACAATCGACGTTCATTGGTACACCAGGAAGCAAAATCTTCACCATATATGGGATAGCAACATAATCGAAACAGCAGAGGGCAAATTCTACGACTTCAGTGTGGATGGCCTAGTTGATGCAATCCAAACGAATATAACGAATGAATGGGCAGATCAAGTTGAAGAATGGGAAAAGTGTGGTTCTGATGAAGTACCATGCACAGAAATATATGCATCAGAAAGTATTCAGGCAGCCTGTGACTGGGCATACAAAGGCGTTTCTGAAGGTTCTACACTAGCAGAGAAATATTTTGCCTCCCGTGTGCCAGTGTTGAACCTACGGTTGGCACAAGGTGGAGTCAGATTAGCAGCTGCTTTAAATCGCATCTTTGGTTCATAG

Protein sequence

MESCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLLTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQAACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS*
Homology
BLAST of Chy10G183520 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.3e-113
Identity = 190/282 (67.38%), Postives = 235/282 (83.33%), Query Frame = 0

Query: 7   LIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLASVCIW 66
           +++  + L++  P   GWG +GH  +CKIAQ+RL + AA AV+ELLPESA+GDL+S+C+W
Sbjct: 11  MMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLW 70

Query: 67  ADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLLTY-NA 126
           ADRVKFRY WS PLH+I+TPD+ C+YQY+RDCKDE+GEKGRCVAGAI NYT+QLL+Y  A
Sbjct: 71  ADRVKFRYHWSSPLHYINTPDA-CSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTA 130

Query: 127 QPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNII 186
             S S+YNLTEALLF+SHFMGDIHQPLHV +  D+GGNTI+VHWYTRK NLHHIWDSNII
Sbjct: 131 ASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNII 190

Query: 187 ETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQAACDWA 246
           ETAE   Y+ +++G+VDA++ NIT EWADQV+ WE C + +  C +IYASE IQAACDWA
Sbjct: 191 ETAEADLYNSALEGMVDALKKNITTEWADQVKRWETC-TKKTACPDIYASEGIQAACDWA 250

Query: 247 YKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFG 288
           YKGV+EG TL ++YF SR+P++  RLAQGGVRLAA LNRIFG
Sbjct: 251 YKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIFG 290

BLAST of Chy10G183520 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.2e-86
Identity = 151/289 (52.25%), Postives = 200/289 (69.20%), Query Frame = 0

Query: 3   SCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLAS 62
           S R     +L L  +   +  WG +GH+TVCKIA+S   +    AV++LLP+SA GDLAS
Sbjct: 4   SLRQWFARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLAS 63

Query: 63  VCIWADRVK--FRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 122
           VC W D +K  +++RW+ PLH++DTPD  C Y+Y RDC D    + RCV GAI NYT QL
Sbjct: 64  VCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQL 123

Query: 123 LTYNAQPSN-SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 182
           ++ +        YNLTEAL+FLSHF+GDIHQPLHVGF GD GGNTI V WY RK NLHH+
Sbjct: 124 MSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHV 183

Query: 183 WDSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQ 242
           WD+ IIE+A   +Y+ S+  +++A+Q N+TN+W++ V  WE C  ++  C   YASESI 
Sbjct: 184 WDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASESIN 243

Query: 243 AACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 289
            AC +AY+  + G+TL + YF SR+P++  RLAQGG+RLAA LNRIF S
Sbjct: 244 LACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSS 292

BLAST of Chy10G183520 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.6e-83
Identity = 146/283 (51.59%), Postives = 194/283 (68.55%), Query Frame = 0

Query: 7   LIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLASVCIW 66
           L++ IL L  +  V   W  +GH   C+IAQ+ L    A  V+ LLP+  +GDL+++C+W
Sbjct: 13  LVLGILILCSVSSVR-SWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCVW 72

Query: 67  ADRVK--FRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLLTYN 126
            D+++  ++YRW+  LH+IDTPD  C+Y+Y RDC D+ G K  CV GAI N+TSQL  Y 
Sbjct: 73  PDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHYG 132

Query: 127 AQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNI 186
              S+  YN+TEALLFLSHFMGDIHQP+HVGFT D GGNTID+ WY  K NLHH+WD  I
Sbjct: 133 EGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDREI 192

Query: 187 IETAEGKFYDFSVDGLVDAIQTNITNE-WADQVEEWEKCGSDEVPCTEIYASESIQAACD 246
           I TA  + YD ++D L + ++ NITN  W D +  W +C +D + C   YASESI+ AC 
Sbjct: 193 ILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTEC-NDLIACPHKYASESIKLACK 252

Query: 247 WAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIF 287
           W YKGV  G TL+E+YF +R+P++  R+ QGGVRLA  LNR+F
Sbjct: 253 WGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVF 293

BLAST of Chy10G183520 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.2e-78
Identity = 146/289 (50.52%), Postives = 188/289 (65.05%), Query Frame = 0

Query: 3   SCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLAS 62
           S R  IV+IL L  +   +  WG  GH+ VCKIAQS   +    AV++LLPESA G+LA+
Sbjct: 4   SLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAA 63

Query: 63  VCIWADRVKF--RYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 122
           VC W D +K   ++RW+  LHF DTPD  C Y+Y RDC      K  CV GAI NYT+QL
Sbjct: 64  VCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQL 123

Query: 123 L-TYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 182
           + T     S   YNLTEAL+FLSH+MGDIHQPLH GF GD GGN I VHWY ++ NLH +
Sbjct: 124 MSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRV 183

Query: 183 WDSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQ 242
           WD  IIE+A   +Y+ S+  ++  +Q  + N W++ V  WE C  ++  C   YASESI 
Sbjct: 184 WDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASESID 243

Query: 243 AACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 289
            AC +AY+  + G+TL + YF SR+PV+  RLAQGG+RLA  LNRIF +
Sbjct: 244 LACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSA 287

BLAST of Chy10G183520 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 3.4e-78
Identity = 139/288 (48.26%), Postives = 191/288 (66.32%), Query Frame = 0

Query: 5   RFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQ-GDLASV 64
           R  IV++L L  +   +  WG DGH+TVCK+A+         AV++LLPES   G LA  
Sbjct: 2   RLWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADF 61

Query: 65  CIWADRVK--FRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLL 124
           C W D +K   +++W+  LH+++TP+  C Y+Y RDC D    K  CV GAI NYT+QL+
Sbjct: 62  CSWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLM 121

Query: 125 TYNAQPSN-SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 184
           + +    N   YNLTEALLFLSH+MGD+HQPLH GF GD GGNTI V+WY  K NLHH+W
Sbjct: 122 SASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVW 181

Query: 185 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 244
           D+ II++A   +Y+ S+  ++ A+Q  + N W++ V  W+ C   +  C  +YASESI  
Sbjct: 182 DNMIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDL 241

Query: 245 ACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 289
           AC +AY+  + G+TL ++YF SR+PV+  RLAQGG+RLAA LNRIF +
Sbjct: 242 ACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFSA 289

BLAST of Chy10G183520 vs. ExPASy TrEMBL
Match: A0A5A7SZC9 (Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001090 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.1e-163
Identity = 277/288 (96.18%), Postives = 281/288 (97.57%), Query Frame = 0

Query: 1   MESCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60
           ME CRFL+VA LSLVF FPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPE AQGDL
Sbjct: 1   MEYCRFLMVAFLSLVFFFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPEFAQGDL 60

Query: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120
           ASVCIWADRVKFRY WS PLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL
Sbjct: 61  ASVCIWADRVKFRYHWSSPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120

Query: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           LTYN QPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTID+HWYTRKQNLHH+W
Sbjct: 121 LTYNTQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDIHWYTRKQNLHHVW 180

Query: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240
           DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA
Sbjct: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 289
           ACDWAYKGVSEGSTLAE+YFASRVPVLNLRLAQGGVRLAAALNRIFGS
Sbjct: 241 ACDWAYKGVSEGSTLAERYFASRVPVLNLRLAQGGVRLAAALNRIFGS 288

BLAST of Chy10G183520 vs. ExPASy TrEMBL
Match: A0A1S3BFG6 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103489294 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.1e-163
Identity = 277/288 (96.18%), Postives = 281/288 (97.57%), Query Frame = 0

Query: 1   MESCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60
           ME CRFL+VA LSLVF FPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPE AQGDL
Sbjct: 1   MEYCRFLMVAFLSLVFFFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPEFAQGDL 60

Query: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120
           ASVCIWADRVKFRY WS PLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL
Sbjct: 61  ASVCIWADRVKFRYHWSSPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120

Query: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           LTYN QPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTID+HWYTRKQNLHH+W
Sbjct: 121 LTYNTQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDIHWYTRKQNLHHVW 180

Query: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240
           DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA
Sbjct: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 289
           ACDWAYKGVSEGSTLAE+YFASRVPVLNLRLAQGGVRLAAALNRIFGS
Sbjct: 241 ACDWAYKGVSEGSTLAERYFASRVPVLNLRLAQGGVRLAAALNRIFGS 288

BLAST of Chy10G183520 vs. ExPASy TrEMBL
Match: A0A6J1DBY6 (Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111018689 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.4e-135
Identity = 232/286 (81.12%), Postives = 251/286 (87.76%), Query Frame = 0

Query: 1   MESCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60
           ME+C+ L+ A+L L FIFPV  GWG+DGH  VCKIAQSRLS+AAADAVQ+LLP+SA GDL
Sbjct: 1   METCKVLVAALLLLGFIFPVISGWGVDGHAAVCKIAQSRLSRAAADAVQQLLPDSASGDL 60

Query: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120
           ASVC WADRVKF Y WS  LHFIDTPDSLC+YQYDRDCKD+AG  GRCVAGAINNYTSQL
Sbjct: 61  ASVCSWADRVKFYYHWSSALHFIDTPDSLCSYQYDRDCKDQAGVIGRCVAGAINNYTSQL 120

Query: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           L Y AQPS S+YNLT+ALLFLSHFMGDIHQPLHVGFT DRGGNTIDVHWYTRKQNLHHIW
Sbjct: 121 LKYQAQPSQSKYNLTQALLFLSHFMGDIHQPLHVGFTTDRGGNTIDVHWYTRKQNLHHIW 180

Query: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240
           D +IIETAE KFY+FSVDGLVDAIQ NIT  W + VEEWEKC S+EV CTEIYASESIQA
Sbjct: 181 DDSIIETAEEKFYNFSVDGLVDAIQMNITKGWRNHVEEWEKCSSNEVACTEIYASESIQA 240

Query: 241 ACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIF 287
           ACDWAYKG SEGS L +KYF SR P++NLRLAQGGVRLAA LNRIF
Sbjct: 241 ACDWAYKGASEGSILEDKYFFSRAPIVNLRLAQGGVRLAAVLNRIF 286

BLAST of Chy10G183520 vs. ExPASy TrEMBL
Match: A0A0A0KWG0 (Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_5G609770 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 9.1e-135
Identity = 229/236 (97.03%), Postives = 232/236 (98.31%), Query Frame = 0

Query: 1   MESCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60
           ME CRFLIVA LSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL
Sbjct: 1   MEKCRFLIVAFLSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60

Query: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120
           ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL
Sbjct: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120

Query: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW
Sbjct: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180

Query: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASE 237
           DSNIIETAEGKFYDFSVDGLVDAIQTNI NEWADQVEEWEKCGSDEVPCTE+Y ++
Sbjct: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNIKNEWADQVEEWEKCGSDEVPCTEMYVAQ 236

BLAST of Chy10G183520 vs. ExPASy TrEMBL
Match: A0A6J1G0B6 (Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111449527 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.7e-134
Identity = 226/288 (78.47%), Postives = 258/288 (89.58%), Query Frame = 0

Query: 1   MESCR-FLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGD 60
           M+ CR F+   ++ LVFIFPVSFGWG+DGHFT+CKIAQSRLS+AAADAVQ LLPE A G+
Sbjct: 1   MKPCRAFMAGLLVFLVFIFPVSFGWGLDGHFTICKIAQSRLSRAAADAVQRLLPEYANGE 60

Query: 61  LASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQ 120
           LA+VCIWADR+KFRYRWS  LH+++TP+SLCTYQY RDCKDE G +GRCVAGAINNYTSQ
Sbjct: 61  LANVCIWADRIKFRYRWSSALHYVNTPESLCTYQYARDCKDENGVEGRCVAGAINNYTSQ 120

Query: 121 LLTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 180
           LLTY  + S+S+YNLTEALLFLSHFMGDIHQPLHVGF  D+GGNTI+VHWYTRKQNLHHI
Sbjct: 121 LLTYKTRSSSSQYNLTEALLFLSHFMGDIHQPLHVGFAEDKGGNTIEVHWYTRKQNLHHI 180

Query: 181 WDSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQ 240
           WDSNIIETAE KFYD SVDGL+DAIQTNIT EW D+VEEWEKC S ++ CT+IYASESI+
Sbjct: 181 WDSNIIETAEEKFYDSSVDGLIDAIQTNITKEWTDEVEEWEKCNSKKIACTDIYASESIR 240

Query: 241 AACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFG 288
           AACDWAYKGV+EG+TLA +YF++RVPV+ LRLAQGGVRLAA LN IFG
Sbjct: 241 AACDWAYKGVTEGATLAAQYFSTRVPVVYLRLAQGGVRLAAVLNSIFG 288

BLAST of Chy10G183520 vs. NCBI nr
Match: XP_004135088.1 (endonuclease 2 [Cucumis sativus] >KAE8648791.1 hypothetical protein Csa_008616 [Cucumis sativus])

HSP 1 Score: 589 bits (1518), Expect = 2.34e-212
Identity = 284/288 (98.61%), Postives = 284/288 (98.61%), Query Frame = 0

Query: 1   MESCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60
           ME CRFLIVA LSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL
Sbjct: 1   MEKCRFLIVAFLSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60

Query: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120
           ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL
Sbjct: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120

Query: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW
Sbjct: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180

Query: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240
           DSNIIETAEGKFYDFSVDGLVDAIQTNI NEWADQVEEWEKCGSDEVPCTEIYASESIQA
Sbjct: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNIKNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 288
           ACDWAYKGVSEGSTLAEKYFASRVPVL LRLAQGGVRLAAALNRIFGS
Sbjct: 241 ACDWAYKGVSEGSTLAEKYFASRVPVLKLRLAQGGVRLAAALNRIFGS 288

BLAST of Chy10G183520 vs. NCBI nr
Match: XP_008446611.1 (PREDICTED: endonuclease 2 [Cucumis melo] >KAA0034565.1 endonuclease 2 [Cucumis melo var. makuwa] >TYK09118.1 endonuclease 2 [Cucumis melo var. makuwa])

HSP 1 Score: 579 bits (1493), Expect = 1.52e-208
Identity = 277/288 (96.18%), Postives = 281/288 (97.57%), Query Frame = 0

Query: 1   MESCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60
           ME CRFL+VA LSLVF FPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPE AQGDL
Sbjct: 1   MEYCRFLMVAFLSLVFFFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPEFAQGDL 60

Query: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120
           ASVCIWADRVKFRY WS PLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL
Sbjct: 61  ASVCIWADRVKFRYHWSSPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120

Query: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           LTYN QPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTID+HWYTRKQNLHH+W
Sbjct: 121 LTYNTQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDIHWYTRKQNLHHVW 180

Query: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240
           DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA
Sbjct: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 288
           ACDWAYKGVSEGSTLAE+YFASRVPVLNLRLAQGGVRLAAALNRIFGS
Sbjct: 241 ACDWAYKGVSEGSTLAERYFASRVPVLNLRLAQGGVRLAAALNRIFGS 288

BLAST of Chy10G183520 vs. NCBI nr
Match: XP_038892859.1 (endonuclease 2 [Benincasa hispida])

HSP 1 Score: 543 bits (1400), Expect = 2.20e-194
Identity = 257/287 (89.55%), Postives = 271/287 (94.43%), Query Frame = 0

Query: 1   MESCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60
           MESCR L++AI+SLVFIFPVSFGWGIDGHFTVC+IAQSRLS+AA DAVQELLP  A GDL
Sbjct: 1   MESCRVLVMAIVSLVFIFPVSFGWGIDGHFTVCRIAQSRLSQAAEDAVQELLPHYANGDL 60

Query: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120
           ASVCIWADR+KFRY WS  LHFIDTPDSLCTYQYDRDCKDE GEKGRCVAGAINNYTSQL
Sbjct: 61  ASVCIWADRIKFRYHWSSALHFIDTPDSLCTYQYDRDCKDEGGEKGRCVAGAINNYTSQL 120

Query: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           L+Y  QPS+SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHH+W
Sbjct: 121 LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVW 180

Query: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240
           DSNIIET+E KFYDFSVDGL+DAIQTNIT EWADQVEEWE CGS+EVPCTEIYASESIQA
Sbjct: 181 DSNIIETSEKKFYDFSVDGLIDAIQTNITKEWADQVEEWENCGSNEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFG 287
           ACDWAYKGV EGSTLAEKYF+SRVP +NLRLAQGGVRLAAALNRIFG
Sbjct: 241 ACDWAYKGVYEGSTLAEKYFSSRVPEVNLRLAQGGVRLAAALNRIFG 287

BLAST of Chy10G183520 vs. NCBI nr
Match: XP_022150586.1 (endonuclease 2 [Momordica charantia])

HSP 1 Score: 487 bits (1253), Expect = 5.52e-172
Identity = 232/286 (81.12%), Postives = 251/286 (87.76%), Query Frame = 0

Query: 1   MESCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60
           ME+C+ L+ A+L L FIFPV  GWG+DGH  VCKIAQSRLS+AAADAVQ+LLP+SA GDL
Sbjct: 1   METCKVLVAALLLLGFIFPVISGWGVDGHAAVCKIAQSRLSRAAADAVQQLLPDSASGDL 60

Query: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120
           ASVC WADRVKF Y WS  LHFIDTPDSLC+YQYDRDCKD+AG  GRCVAGAINNYTSQL
Sbjct: 61  ASVCSWADRVKFYYHWSSALHFIDTPDSLCSYQYDRDCKDQAGVIGRCVAGAINNYTSQL 120

Query: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           L Y AQPS S+YNLT+ALLFLSHFMGDIHQPLHVGFT DRGGNTIDVHWYTRKQNLHHIW
Sbjct: 121 LKYQAQPSQSKYNLTQALLFLSHFMGDIHQPLHVGFTTDRGGNTIDVHWYTRKQNLHHIW 180

Query: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240
           D +IIETAE KFY+FSVDGLVDAIQ NIT  W + VEEWEKC S+EV CTEIYASESIQA
Sbjct: 181 DDSIIETAEEKFYNFSVDGLVDAIQMNITKGWRNHVEEWEKCSSNEVACTEIYASESIQA 240

Query: 241 ACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIF 286
           ACDWAYKG SEGS L +KYF SR P++NLRLAQGGVRLAA LNRIF
Sbjct: 241 ACDWAYKGASEGSILEDKYFFSRAPIVNLRLAQGGVRLAAVLNRIF 286

BLAST of Chy10G183520 vs. NCBI nr
Match: XP_022945222.1 (endonuclease 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 483 bits (1243), Expect = 1.91e-170
Identity = 226/288 (78.47%), Postives = 258/288 (89.58%), Query Frame = 0

Query: 1   MESCR-FLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGD 60
           M+ CR F+   ++ LVFIFPVSFGWG+DGHFT+CKIAQSRLS+AAADAVQ LLPE A G+
Sbjct: 1   MKPCRAFMAGLLVFLVFIFPVSFGWGLDGHFTICKIAQSRLSRAAADAVQRLLPEYANGE 60

Query: 61  LASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQ 120
           LA+VCIWADR+KFRYRWS  LH+++TP+SLCTYQY RDCKDE G +GRCVAGAINNYTSQ
Sbjct: 61  LANVCIWADRIKFRYRWSSALHYVNTPESLCTYQYARDCKDENGVEGRCVAGAINNYTSQ 120

Query: 121 LLTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 180
           LLTY  + S+S+YNLTEALLFLSHFMGDIHQPLHVGF  D+GGNTI+VHWYTRKQNLHHI
Sbjct: 121 LLTYKTRSSSSQYNLTEALLFLSHFMGDIHQPLHVGFAEDKGGNTIEVHWYTRKQNLHHI 180

Query: 181 WDSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQ 240
           WDSNIIETAE KFYD SVDGL+DAIQTNIT EW D+VEEWEKC S ++ CT+IYASESI+
Sbjct: 181 WDSNIIETAEEKFYDSSVDGLIDAIQTNITKEWTDEVEEWEKCNSKKIACTDIYASESIR 240

Query: 241 AACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFG 287
           AACDWAYKGV+EG+TLA +YF++RVPV+ LRLAQGGVRLAA LN IFG
Sbjct: 241 AACDWAYKGVTEGATLAAQYFSTRVPVVYLRLAQGGVRLAAVLNSIFG 288

BLAST of Chy10G183520 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 409.1 bits (1050), Expect = 3.0e-114
Identity = 190/282 (67.38%), Postives = 235/282 (83.33%), Query Frame = 0

Query: 7   LIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLASVCIW 66
           +++  + L++  P   GWG +GH  +CKIAQ+RL + AA AV+ELLPESA+GDL+S+C+W
Sbjct: 11  MMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLW 70

Query: 67  ADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLLTY-NA 126
           ADRVKFRY WS PLH+I+TPD+ C+YQY+RDCKDE+GEKGRCVAGAI NYT+QLL+Y  A
Sbjct: 71  ADRVKFRYHWSSPLHYINTPDA-CSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTA 130

Query: 127 QPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNII 186
             S S+YNLTEALLF+SHFMGDIHQPLHV +  D+GGNTI+VHWYTRK NLHHIWDSNII
Sbjct: 131 ASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNII 190

Query: 187 ETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQAACDWA 246
           ETAE   Y+ +++G+VDA++ NIT EWADQV+ WE C + +  C +IYASE IQAACDWA
Sbjct: 191 ETAEADLYNSALEGMVDALKKNITTEWADQVKRWETC-TKKTACPDIYASEGIQAACDWA 250

Query: 247 YKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFG 288
           YKGV+EG TL ++YF SR+P++  RLAQGGVRLAA LNRIFG
Sbjct: 251 YKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIFG 290

BLAST of Chy10G183520 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 321.2 bits (822), Expect = 8.3e-88
Identity = 151/289 (52.25%), Postives = 200/289 (69.20%), Query Frame = 0

Query: 3   SCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLAS 62
           S R     +L L  +   +  WG +GH+TVCKIA+S   +    AV++LLP+SA GDLAS
Sbjct: 4   SLRQWFARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLAS 63

Query: 63  VCIWADRVK--FRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 122
           VC W D +K  +++RW+ PLH++DTPD  C Y+Y RDC D    + RCV GAI NYT QL
Sbjct: 64  VCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQL 123

Query: 123 LTYNAQPSN-SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 182
           ++ +        YNLTEAL+FLSHF+GDIHQPLHVGF GD GGNTI V WY RK NLHH+
Sbjct: 124 MSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHV 183

Query: 183 WDSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQ 242
           WD+ IIE+A   +Y+ S+  +++A+Q N+TN+W++ V  WE C  ++  C   YASESI 
Sbjct: 184 WDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASESIN 243

Query: 243 AACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 289
            AC +AY+  + G+TL + YF SR+P++  RLAQGG+RLAA LNRIF S
Sbjct: 244 LACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSS 292

BLAST of Chy10G183520 vs. TAIR 10
Match: AT1G11190.1 (bifunctional nuclease i )

HSP 1 Score: 310.8 bits (795), Expect = 1.1e-84
Identity = 146/283 (51.59%), Postives = 194/283 (68.55%), Query Frame = 0

Query: 7   LIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLASVCIW 66
           L++ IL L  +  V   W  +GH   C+IAQ+ L    A  V+ LLP+  +GDL+++C+W
Sbjct: 13  LVLGILILCSVSSVR-SWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCVW 72

Query: 67  ADRVK--FRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQLLTYN 126
            D+++  ++YRW+  LH+IDTPD  C+Y+Y RDC D+ G K  CV GAI N+TSQL  Y 
Sbjct: 73  PDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHYG 132

Query: 127 AQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNI 186
              S+  YN+TEALLFLSHFMGDIHQP+HVGFT D GGNTID+ WY  K NLHH+WD  I
Sbjct: 133 EGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDREI 192

Query: 187 IETAEGKFYDFSVDGLVDAIQTNITNE-WADQVEEWEKCGSDEVPCTEIYASESIQAACD 246
           I TA  + YD ++D L + ++ NITN  W D +  W +C +D + C   YASESI+ AC 
Sbjct: 193 ILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTEC-NDLIACPHKYASESIKLACK 252

Query: 247 WAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIF 287
           W YKGV  G TL+E+YF +R+P++  R+ QGGVRLA  LNR+F
Sbjct: 253 WGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVF 293

BLAST of Chy10G183520 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 294.7 bits (753), Expect = 8.3e-80
Identity = 146/289 (50.52%), Postives = 188/289 (65.05%), Query Frame = 0

Query: 3   SCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLAS 62
           S R  IV+IL L  +   +  WG  GH+ VCKIAQS   +    AV++LLPESA G+LA+
Sbjct: 4   SLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAA 63

Query: 63  VCIWADRVKF--RYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 122
           VC W D +K   ++RW+  LHF DTPD  C Y+Y RDC      K  CV GAI NYT+QL
Sbjct: 64  VCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQL 123

Query: 123 L-TYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 182
           + T     S   YNLTEAL+FLSH+MGDIHQPLH GF GD GGN I VHWY ++ NLH +
Sbjct: 124 MSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRV 183

Query: 183 WDSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQ 242
           WD  IIE+A   +Y+ S+  ++  +Q  + N W++ V  WE C  ++  C   YASESI 
Sbjct: 184 WDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASESID 243

Query: 243 AACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 289
            AC +AY+  + G+TL + YF SR+PV+  RLAQGG+RLA  LNRIF +
Sbjct: 244 LACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSA 287

BLAST of Chy10G183520 vs. TAIR 10
Match: AT4G21590.2 (endonuclease 3 )

HSP 1 Score: 294.7 bits (753), Expect = 8.3e-80
Identity = 146/289 (50.52%), Postives = 188/289 (65.05%), Query Frame = 0

Query: 3   SCRFLIVAILSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDLAS 62
           S R  IV+IL L  +   +  WG  GH+ VCKIAQS   +    AV++LLPESA G+LA+
Sbjct: 4   SLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAA 63

Query: 63  VCIWADRVKF--RYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 122
           VC W D +K   ++RW+  LHF DTPD  C Y+Y RDC      K  CV GAI NYT+QL
Sbjct: 64  VCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQL 123

Query: 123 L-TYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 182
           + T     S   YNLTEAL+FLSH+MGDIHQPLH GF GD GGN I VHWY ++ NLH +
Sbjct: 124 MSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRV 183

Query: 183 WDSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQ 242
           WD  IIE+A   +Y+ S+  ++  +Q  + N W++ V  WE C  ++  C   YASESI 
Sbjct: 184 WDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASESID 243

Query: 243 AACDWAYKGVSEGSTLAEKYFASRVPVLNLRLAQGGVRLAAALNRIFGS 289
            AC +AY+  + G+TL + YF SR+PV+  RLAQGG+RLA  LNRIF +
Sbjct: 244 LACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSA 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9G44.3e-11367.38Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1[more]
F4JJL01.2e-8652.25Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1[more]
Q9SXA61.6e-8351.59Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1[more]
Q8LDW61.2e-7850.52Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1[more]
F4JJL33.4e-7848.26Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SZC96.1e-16396.18Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3BFG66.1e-16396.18Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103489294 PE=3 SV=1[more]
A0A6J1DBY65.4e-13581.12Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111018689 PE=3 SV... [more]
A0A0A0KWG09.1e-13597.03Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_5G609770 PE=3 SV=1[more]
A0A6J1G0B67.7e-13478.47Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111449527 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_004135088.12.34e-21298.61endonuclease 2 [Cucumis sativus] >KAE8648791.1 hypothetical protein Csa_008616 [... [more]
XP_008446611.11.52e-20896.18PREDICTED: endonuclease 2 [Cucumis melo] >KAA0034565.1 endonuclease 2 [Cucumis m... [more]
XP_038892859.12.20e-19489.55endonuclease 2 [Benincasa hispida][more]
XP_022150586.15.52e-17281.12endonuclease 2 [Momordica charantia][more]
XP_022945222.11.91e-17078.47endonuclease 2 isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G68290.13.0e-11467.38endonuclease 2 [more]
AT4G21585.18.3e-8852.25endonuclease 4 [more]
AT1G11190.11.1e-8451.59bifunctional nuclease i [more]
AT4G21590.18.3e-8050.52endonuclease 3 [more]
AT4G21590.28.3e-8050.52endonuclease 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003154S1/P1 nucleasePFAMPF02265S1-P1_nucleasecoord: 24..286
e-value: 3.9E-82
score: 275.9
IPR003154S1/P1 nucleasePANTHERPTHR33146ENDONUCLEASE 4coord: 7..287
IPR003154S1/P1 nucleaseCDDcd11010S1-P1_nucleasecoord: 24..286
e-value: 1.81619E-90
score: 267.328
IPR008947Phospholipase C/P1 nuclease domain superfamilyGENE3D1.10.575.10P1 Nucleasecoord: 24..288
e-value: 4.0E-104
score: 350.1
IPR008947Phospholipase C/P1 nuclease domain superfamilySUPERFAMILY48537Phospholipase C/P1 nucleasecoord: 24..285
NoneNo IPR availablePANTHERPTHR33146:SF2ENDONUCLEASE 2coord: 7..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G183520.1Chy10G183520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006308 DNA catabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0004521 endoribonuclease activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000014 single-stranded DNA endodeoxyribonuclease activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds