Chy10G178620 (gene) Cucumber (hystrix) v1

Overview
NameChy10G178620
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionProlyl endopeptidase
LocationchrH10: 13141949 .. 13148022 (-)
RNA-Seq ExpressionChy10G178620
SyntenyChy10G178620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGCCTTTGCAACAAAACGGCATATTTGGTATACTTAGAAGAAGCTTCGTTTTCCTTATTCCCGTACTCTACCTTTCGCCGGCACTTCCGTCGGTAGCTTCATTCCGTCACTTCCGATCACCGGTTGCTACAATGAGTCAATCTCACTCTCCTCCGGTCGCCAACAAGGTGGAGAATAAAATGGAGTTGTTCGGAGACGTAAGGATCGACAACTATTACTGGTTACGTGACGATTCTCGCTCAAATCCTGATGTCCTTTCGTATCTGCGGGAGGAGAATGCCTATACCGATTCCGTCATGACCGGTGAACTTTAGTTTACATTTTCATTTCGGTTCTTGAATTTTTTTTTTGGTTACTACGTGAGAAATTTATGAATCAGTGATATGGTAGCATTGATTTGATTTGAATGAGAGTAACGTCGTTTGGTTTATCGATTGCTGATGATTTGTGTGTAATGTTGATTTTTTGTTTATTAAACGATCTAGGAACAAAGAAAGTGGAAGACCAGATTTTTAGTGAGATAAGAGGACGAATCAAGGAGGATGATATAACTGTGCCTGAACGGAAAGGTTCTTACTACTACTATGAGAGGACTTTGGAAGGGAAAGAATATGTTCAATATTGTAGGCGTTTTGTTCCTCGTGGTGAAGAAGAAGCTATATCTGTGCACGATACCATGCCCACTGGACCCAATGCTCCTCCGGAGCATGTTATATTGGATGAAAATGTTAAGGCCGAGAATCAATCTTACTACTCCATTGGTTGCTTTGAGGTATTGTAAAGACGGAACTGCGTCAGAATTTGATCATGTTTTCTCTTGGACAGAATTTTGATTCAAGGATCTTTATCGTCGTTGCTTCTCCAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTTTATATAATCGATGCCGAGACTGGCGCTCCTGTGGGAAAGCCTCTTGTAGGTGTGACCTCGTATCTCAAATGGGCTGGTGATGATGCTTTAGTTTACATCACAATGGATGAGATTCTTCGACCTGATAAGGTGATTTACTGATTTTCTACCCTACATTCTGTACTTCTCATGGCTTTGACTGTTGTTTTCTCATTGGAAAAACAACTACTATCATGGGGTCTATAATAGGGTCTCTTCCCTTGTGGAATTGAAATACGAAGAAGACCGAATTTTAATCGAGGAGGAAATAGTATTGCAAGGTCTTGAAGAGGACCTCCTAGACCATCCCGTCTGATATCATCATAGATCATCAATTATTGTTGATTGTTCTGTGCATTCAGAATCGCCTTCTAAAATGATATAGTAGGTTATTTCACTAAAAGGAAAATTCCTTACATACCATTAAATAGCTTGCATTCCTCTAGTTTTTCATTTGTAATACTGATATATAGATTATAGTATCCTTATGATTTGATATCAGAAGAGTGGAGGCAGAGGAGTTTTTAGAGTTGTAAAGAGCGCTGCTCAAAGTACAAATTTTTGGTGAAAATGCATTCAGTATCTGCATTCTGATGTTCCCAGTAATATTATCGTTGAACTTCAAATTGATAATTTCTTTTCAACGTTTTCAGAATTTTAGATGTTGATCTAGTAAACAATTTCCTCTTTCAATAGGCATGGTTACATAAACTGGGAACAGAGCAGTCAATGGACACCTGCCTTTATCATGAAAAGGATGACATGTTCTCTCTTGATCTTGAAGCTTCTGAGAGCAAGAAATATTTGTTTATTGCATCTGAAAGTAAATTTACTCGGTTCAACTTTTATCTTGATGTATCAAGGCCCGAAGATGGGCTTGTTGTCCTGACACCTAGAGTGGATGGAATTGACACTTATCCCAGTCATCGTGGAAATCATTTTTTTATCCTGAGACGAAGTGAAGAGATTTTTAATTCAGAAGTAGTAGCTTGCCCTCTTGATAATACATCAGCTACCACAGTTATTCTTCCGCACAGGGAAAGGTAGTGTTTTGTATTCTTTTATGATAATTGATGATTGACCTGATGGTATTATGTGAAGTTGAAACATAATTGAAAGTGATGAGCTGATAGTTAATGACTTGAATCAACAAGCTTTAAAAGAAATCTTTGTCGGAACTTGGTGTTTATTTATTTAATTTGTTTGGTACGTTGTTGGATTTATTCAACAAATTTATAAACCTTATTGTGCTGTGAATCTGATTTTTTCTATAAGAAATGTTAAAAGCTTCTTAATTAGCTTGTTACTGGGGCTTATGAAGTTGTTTGAATACTGTTCAGTGTGAAAATCCAGGAAATCGAACTATTTCTTAACCACATCGTCATATTGGAACGTGAAGATGGTCTGCCAAAAGTTGTTGTCTATAGCCTTCCAGATATTGGAGAACCACTTAAAACCCTTGAAGGAGGACGAGCTGTGGATTTTGCTGATGCTACTTATTCAGTGGATGTTTTAGAATCAGAATTCTCTTCTAGTATTTTACGGTTTTGTTACAGCTCAATGAAGACACCCCCCTCTACATATGATTATGATATGAAAACAGGAGTTTCCGTTCTAAAGAAAGTTGAAACAGTGAGTAGTTCCACAATCATGGTTATGTTGTTCTCTTATGACCGGGATTGCCTCTAGGTTTAAATGAATTATTGTTAACCTTTACATGTGATATTTTCAAATTTCCTGGTCATATTATAAAAATATATATAAACCAGATATGAATTTCTATTCTCTGTTTTCTCAACCTATTACTACCCATTTGTGGCTAGGAGTATATTTCATTTATGTATCTAGTCTTTCTAAAACCAGTGCTAACTTCAACTTTGAGTACATCCCTTTGCTTCCTTTGTTTCTTTTTCTTTCATTTCTTACGATTAAGAATTTTTATTCCCTATATGCTAGTGATAGTTTAGAAAAATCTTCCGTCTGTCTCTTATGCCTTTTCATCGTCCGTTTTTTGTTGTTGTTGTTGAGAATTCTTGTTGTCTAGCGAATTTAAAAAGTCTCCTTACTTTTCCTTCCCCCCAGGAGGATTTGAATTAATTCATCTTATTGTAACGTAGTGAAATTTCCACAAATTTTCTCAGGTGTTGGGAGGTTTTGATTCTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTTTAGATGGCACTAAAGTTCCCCTATCAATTGTTTACCGAAAGGATCTAGTGAAACTGGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTATGAGGTAACATGTCTTGTGTTAATTAGGCTCTATACGGATCTCAGGAACTCGAAGTTATTTTAAATTGAAAAGAGTGTTTTTGTTTCCTGGGGACTCCCCCTATGAGTCAGAGTTTCTTTATTGGAGCTTCAAATAATGTGATTATAAATTTATAAGTTATTTTCCTGTTTTAATTGATTTATCAAAATTTCCAATTCTGAAATATTTATTTCTGCATTGTAGTGGTTCTTTGAGGATCTATGTTTCGTTTTGATGTCCAGTGTTTTTGCTATAATTACGATCTCTGCGTTGCTAATTTACTGTTCTTAAACGTTTGAGGACATGAACTTGTACCTGAATCTTTTTGTTTACTTTTTTCTTGTCACTTAATTGAGTTTTGAATTGTAACAATGATGGTACATGAACCTGATCTCGAAATGAATTTCATTCTTTGAACAGTATTGCATAGACCCTAGTTTCAAGGCATCGAGGATATCTTTGTTAGACAGAGGTTTTATTTATGTAATAGCTCACATTCGCGGGGGTGGTGAAATGGGGAGGCAGTGGTATGAAAATGGAAAGTTACTGAAGAAAAAAAATACATTCACTGATTTTATTTCTTCTGCTGAATACTTGATCGAGAATAAATACTGTTCAAAGGAAAAACTATGCATCAATGGAAGAAGTGCTGGTGGTTTGCTTATTGGTGCTGTTATAAATATGAGGCCAGATTTGTTCAAAGCTGCAATTGCTGGGGTACCTTTTGTAGATGTTCTAACGACCATGCTTGATCCCACAATTCCTCTTACAACTTCGGAGTGGGAGGTAAATCTTCCCTAGCTAGTGTACCTTTCGCAATTCTGTAGGTGACATGCAGTGAAGTAGTGCAATTCCTTGGTCAGTACTTAAATAGACAAGTTAAGAACTCTTTAACTTTTAGCTAATGACATTGGCTCAAAATCACTTTTGAGTCCCCAAATTATGGTGCCATTTTGAACTAGAGTCCAATGTAGGATAGGCAGTTATCAGTTTAACAATATATGTATTGAAAAAAACAAAAGGGTGGAAAGTGTCCCTGTTTGTAGCACGGTTCCTAACATACGAGATTTTCTTTTGACTCTGTAAATATCTTTACTTATCCATTATCTTATCTTTTTTCACTTTTTTCATCTGTCGTTTACTGAAAATATGTTGTATAACTAATTGCAGCGCCTATTCTTTTTGACTTGCACTTACCTTCTTTTGAATTCTGCAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCTCCGGTTGATAATGTACGCACCTCACTATTTTTTAATTAACATATTCTGTTTCAGCCATTTCCTTCTAATCAATCATCCCCTGATTTTGGATCATTTGAATGTGTCCGTGCTTATGTGTTTCATAATTCATAGAATATCTTCAAACTTTTGTTTTGATTAAGACATGACTTTATCTTAATGTTTCAGGTTAAGGCTCAAAATTATCCGGACGTACTTGTTACTGCCGGCTTAAACGGTAAGTGTGATGGTTTAAAACTTCAATTCGAGTAGAGCCCCAATCAATTTTCCTGTTTCTTTATCCTGTGCACACATTCATTCTTCATATCCAGAATAGACAAGGTCACAAGGAGATTGATGAATATATGGATGTCTGGGAGTGATTTTGGTCACGTTGAAAATCATTCAAAGCATGCTTTTACACTTCAAATTAGATTAATATTTCATTTTATGCTTTTAAATGTAATTTTCATATCATACAAAATAATTGATTTTATTTTGAATGATGAAAAGCATTTTTACAGTGATTTTAAAAATGTTAAAAGTGGTTTGAATCCTTTCAAAATCACACTCCAGGTGTGCCCAATCAAACATTCCTTCAAAATGTTAAATCCTTTATTTGGTCCATTGGTCTATGTGGCAGATCCACGTGTCTTATATTCAGAACCGGCTAAGTTTGTGGCAAAATTAAGGGCTACGAAGACTGATAATAATCTTCTGCTTTTCAAATGTGAACTTGGTGCTGGACATTTTTCAAAATCAGGAAGGTAATTATTCGATTGTTGATTTCTTTTGTGGGCTTCGTGATCTGTGTTTTCTTTCTTGTTTGTATCTCCTCTCTCTTTTTGTTGCATGTGCCTTTTCTTCTTCTTCAACATGATTATAAAATTATTTCTTATTTAATTGATGATATTCACTGCTGCCTTTTCTATTTGTAACTCTACGGTATACATGGAGAAGAAACATTATTTTGAGCTCTGTTATGATTTCTCACGTCCTATTCATATACCTGTAACTTTTTTGGCAGAACATTATTATTTTTCATACTAGTGTAGGACTTTTTTATGAAGTATGAAGAAAAGAAAGAAAAAGCTATTCTAAAACAGACCTTTAGTTAGGTCTTATTTTCTTGGATTTAAGTCATTTTCTTAATTAATGTAGGACTCCTTTTTGTAGAGTGGTTTTTTTTAATACTTTGTATGTTCATTCATTTCTCTGAGTTTCTATTATTTTTTTGCAAAAGTTGGCCACTCGTGACAATAAACTTAGGAAAAGATAAAGGAAAACAACCTTGAGAATACAAAAAGAACCTCTTTACATTAATCTTTTGTTATTAAAAAAATAAAGAGCTCATCCTTCGTTGTTAGGGGTCGATGTTCTGGAGCATTTAAAAGATACTTACATTTTCTGGTTTTTGATTCATTATTATTATTATTATTCTTTTGCTGCCATTTCAGATTTGAGAAGCTTCAAGAGGATGCATTCACATACGCTTTTATACTGAAGTCTCTTAACATGATTCCAGCAGTCGGAAATTGA

mRNA sequence

ATGACGCCTTTGCAACAAAACGGCATATTTGGTATACTTAGAAGAAGCTTCGTTTTCCTTATTCCCGTACTCTACCTTTCGCCGGCACTTCCGTCGGTAGCTTCATTCCGTCACTTCCGATCACCGGTTGCTACAATGAGTCAATCTCACTCTCCTCCGGTCGCCAACAAGGTGGAGAATAAAATGGAGTTGTTCGGAGACGTAAGGATCGACAACTATTACTGGTTACGTGACGATTCTCGCTCAAATCCTGATGTCCTTTCGTATCTGCGGGAGGAGAATGCCTATACCGATTCCGTCATGACCGGAACAAAGAAAGTGGAAGACCAGATTTTTAGTGAGATAAGAGGACGAATCAAGGAGGATGATATAACTGTGCCTGAACGGAAAGGTTCTTACTACTACTATGAGAGGACTTTGGAAGGGAAAGAATATGTTCAATATTGTAGGCGTTTTGTTCCTCGTGGTGAAGAAGAAGCTATATCTGTGCACGATACCATGCCCACTGGACCCAATGCTCCTCCGGAGCATGTTATATTGGATGAAAATGTTAAGGCCGAGAATCAATCTTACTACTCCATTGGTTGCTTTGAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTTTATATAATCGATGCCGAGACTGGCGCTCCTGTGGGAAAGCCTCTTGTAGGTGTGACCTCGTATCTCAAATGGGCTGGTGATGATGCTTTAGTTTACATCACAATGGATGAGATTCTTCGACCTGATAAGGCATGGTTACATAAACTGGGAACAGAGCAGTCAATGGACACCTGCCTTTATCATGAAAAGGATGACATGTTCTCTCTTGATCTTGAAGCTTCTGAGAGCAAGAAATATTTGTTTATTGCATCTGAAAGTAAATTTACTCGGTTCAACTTTTATCTTGATGTATCAAGGCCCGAAGATGGGCTTGTTGTCCTGACACCTAGAGTGGATGGAATTGACACTTATCCCAGTCATCGTGGAAATCATTTTTTTATCCTGAGACGAAGTGAAGAGATTTTTAATTCAGAAGTAGTAGCTTGCCCTCTTGATAATACATCAGCTACCACAGTTATTCTTCCGCACAGGGAAAGTGTGAAAATCCAGGAAATCGAACTATTTCTTAACCACATCGTCATATTGGAACGTGAAGATGGTCTGCCAAAAGTTGTTGTCTATAGCCTTCCAGATATTGGAGAACCACTTAAAACCCTTGAAGGAGGACGAGCTGTGGATTTTGCTGATGCTACTTATTCAGTGGATGTTTTAGAATCAGAATTCTCTTCTAGTATTTTACGGTTTTGTTACAGCTCAATGAAGACACCCCCCTCTACATATGATTATGATATGAAAACAGGAGTTTCCGTTCTAAAGAAAGTTGAAACAGTGTTGGGAGGTTTTGATTCTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTTTAGATGGCACTAAAGTTCCCCTATCAATTGTTTACCGAAAGGATCTAGTGAAACTGGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTATGAGTATTGCATAGACCCTAGTTTCAAGGCATCGAGGATATCTTTGTTAGACAGAGGTTTTATTTATGTAATAGCTCACATTCGCGGGGGTGGTGAAATGGGGAGGCAGTGGTATGAAAATGGAAAGTTACTGAAGAAAAAAAATACATTCACTGATTTTATTTCTTCTGCTGAATACTTGATCGAGAATAAATACTGTTCAAAGGAAAAACTATGCATCAATGGAAGAAGTGCTGGTGGTTTGCTTATTGGTGCTGTTATAAATATGAGGCCAGATTTGTTCAAAGCTGCAATTGCTGGGGTACCTTTTGTAGATGTTCTAACGACCATGCTTGATCCCACAATTCCTCTTACAACTTCGGAGTGGGAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCTCCGGTTGATAATGTTAAGGCTCAAAATTATCCGGACGTACTTGTTACTGCCGGCTTAAACGATCCACGTGTCTTATATTCAGAACCGGCTAAGTTTGTGGCAAAATTAAGGGCTACGAAGACTGATAATAATCTTCTGCTTTTCAAATGTGAACTTGGTGCTGGACATTTTTCAAAATCAGGAAGATTTGAGAAGCTTCAAGAGGATGCATTCACATACGCTTTTATACTGAAGTCTCTTAACATGATTCCAGCAGTCGGAAATTGA

Coding sequence (CDS)

ATGACGCCTTTGCAACAAAACGGCATATTTGGTATACTTAGAAGAAGCTTCGTTTTCCTTATTCCCGTACTCTACCTTTCGCCGGCACTTCCGTCGGTAGCTTCATTCCGTCACTTCCGATCACCGGTTGCTACAATGAGTCAATCTCACTCTCCTCCGGTCGCCAACAAGGTGGAGAATAAAATGGAGTTGTTCGGAGACGTAAGGATCGACAACTATTACTGGTTACGTGACGATTCTCGCTCAAATCCTGATGTCCTTTCGTATCTGCGGGAGGAGAATGCCTATACCGATTCCGTCATGACCGGAACAAAGAAAGTGGAAGACCAGATTTTTAGTGAGATAAGAGGACGAATCAAGGAGGATGATATAACTGTGCCTGAACGGAAAGGTTCTTACTACTACTATGAGAGGACTTTGGAAGGGAAAGAATATGTTCAATATTGTAGGCGTTTTGTTCCTCGTGGTGAAGAAGAAGCTATATCTGTGCACGATACCATGCCCACTGGACCCAATGCTCCTCCGGAGCATGTTATATTGGATGAAAATGTTAAGGCCGAGAATCAATCTTACTACTCCATTGGTTGCTTTGAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTTTATATAATCGATGCCGAGACTGGCGCTCCTGTGGGAAAGCCTCTTGTAGGTGTGACCTCGTATCTCAAATGGGCTGGTGATGATGCTTTAGTTTACATCACAATGGATGAGATTCTTCGACCTGATAAGGCATGGTTACATAAACTGGGAACAGAGCAGTCAATGGACACCTGCCTTTATCATGAAAAGGATGACATGTTCTCTCTTGATCTTGAAGCTTCTGAGAGCAAGAAATATTTGTTTATTGCATCTGAAAGTAAATTTACTCGGTTCAACTTTTATCTTGATGTATCAAGGCCCGAAGATGGGCTTGTTGTCCTGACACCTAGAGTGGATGGAATTGACACTTATCCCAGTCATCGTGGAAATCATTTTTTTATCCTGAGACGAAGTGAAGAGATTTTTAATTCAGAAGTAGTAGCTTGCCCTCTTGATAATACATCAGCTACCACAGTTATTCTTCCGCACAGGGAAAGTGTGAAAATCCAGGAAATCGAACTATTTCTTAACCACATCGTCATATTGGAACGTGAAGATGGTCTGCCAAAAGTTGTTGTCTATAGCCTTCCAGATATTGGAGAACCACTTAAAACCCTTGAAGGAGGACGAGCTGTGGATTTTGCTGATGCTACTTATTCAGTGGATGTTTTAGAATCAGAATTCTCTTCTAGTATTTTACGGTTTTGTTACAGCTCAATGAAGACACCCCCCTCTACATATGATTATGATATGAAAACAGGAGTTTCCGTTCTAAAGAAAGTTGAAACAGTGTTGGGAGGTTTTGATTCTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTTTAGATGGCACTAAAGTTCCCCTATCAATTGTTTACCGAAAGGATCTAGTGAAACTGGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTATGAGTATTGCATAGACCCTAGTTTCAAGGCATCGAGGATATCTTTGTTAGACAGAGGTTTTATTTATGTAATAGCTCACATTCGCGGGGGTGGTGAAATGGGGAGGCAGTGGTATGAAAATGGAAAGTTACTGAAGAAAAAAAATACATTCACTGATTTTATTTCTTCTGCTGAATACTTGATCGAGAATAAATACTGTTCAAAGGAAAAACTATGCATCAATGGAAGAAGTGCTGGTGGTTTGCTTATTGGTGCTGTTATAAATATGAGGCCAGATTTGTTCAAAGCTGCAATTGCTGGGGTACCTTTTGTAGATGTTCTAACGACCATGCTTGATCCCACAATTCCTCTTACAACTTCGGAGTGGGAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCTCCGGTTGATAATGTTAAGGCTCAAAATTATCCGGACGTACTTGTTACTGCCGGCTTAAACGATCCACGTGTCTTATATTCAGAACCGGCTAAGTTTGTGGCAAAATTAAGGGCTACGAAGACTGATAATAATCTTCTGCTTTTCAAATGTGAACTTGGTGCTGGACATTTTTCAAAATCAGGAAGATTTGAGAAGCTTCAAGAGGATGCATTCACATACGCTTTTATACTGAAGTCTCTTAACATGATTCCAGCAGTCGGAAATTGA

Protein sequence

MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVENKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLEGGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCELGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN*
Homology
BLAST of Chy10G178620 vs. ExPASy Swiss-Prot
Match: Q59536 (Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 2.3e-150
Identity = 284/703 (40.40%), Postives = 424/703 (60.31%), Query Frame = 0

Query: 53  PVANKVENKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIF 112
           P+A ++ +  EL GDVR D+YYWL+D  R N +V+ YL EEN Y   +M   ++  +QI+
Sbjct: 4   PIAKRIPHPHELHGDVREDDYYWLKD--RDNTEVIQYLEEENRYYHEIMRPLQEQTEQIY 63

Query: 113 SEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPN 172
             +  R+ + ++ VP + G ++YY R  + K+Y  Y R+   +    A+    T      
Sbjct: 64  ESMVDRVPDSEMKVPVQHGQFFYYSRLDKNKQYPIYARK---QAASRALLQDAT------ 123

Query: 173 APPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVG 232
              E V+LD N  AE   Y S+    ++ ++  +AY E+  G + YT+YI D  TG  + 
Sbjct: 124 ---EEVVLDLNELAEEDDYLSVTVQRMTTDHSRLAYLENRDGTDRYTIYIKDLNTGELLS 183

Query: 233 K--PLVGVTSYLKWAG-DDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSL 292
              P V +   ++W    D + Y T+DE  RP + W H+LG++   D  ++ EKDD F+L
Sbjct: 184 DRVPNVYIYGSMEWCRCGDYIFYTTVDEHQRPCQLWRHRLGSDVESDELIFEEKDDTFTL 243

Query: 293 DLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILR 352
            +  S+S K++F+ S SK T     +D   P   L ++  R DGI     H  +   IL 
Sbjct: 244 FISKSQSGKFIFVYSSSKTTSEIHMIDTDSPLSPLQLVDERRDGILYDVEHWEDDLLIL- 303

Query: 353 RSEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSL 412
            +E   N +++ CPL++ S+   ++ + E   +QE+  F + ++I  RE+GL ++ V   
Sbjct: 304 TNEGALNFQLLRCPLNDLSSKVNVVEYNEERYLQEMYPFRDKLLIAGRENGLTQIWVVHD 363

Query: 413 PDIGEPLKTLEGGRAVDFADATYSVDVL-ESEFSSSILRFCYSSMKTPPSTYDYDMKTGV 472
            ++          + + + +  Y+V VL E  + ++ +   Y S+ TP +T+  +++TG 
Sbjct: 364 GEL----------QQISWDEPLYTVAVLSEQSYDTNEVLIQYESLLTPKTTFGLNLQTGE 423

Query: 473 SVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSY 532
               +V  V G +D +++  E+ WAT   G KVP++ VY +  +  +G  PL+LYGYGSY
Sbjct: 424 KQCLQVAPVSGEYDRSQFRQEQLWATGRSGVKVPMTAVYLEGALD-NGPAPLILYGYGSY 483

Query: 533 EYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYL 592
               DP F   R+ LL++G ++V A +RGG EMGR WYE+GK+  K+NTFTDFI++A++L
Sbjct: 484 GSNSDPRFDPYRLPLLEKGIVFVTAQVRGGSEMGRGWYEDGKMQNKRNTFTDFIAAAKHL 543

Query: 593 IENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTS 652
           I+  Y S  K+   G SAGGLL+GAV NM  +LFK  +  VPFVDV+TTMLD +IPLTT 
Sbjct: 544 IDQNYTSPTKMAARGGSAGGLLVGAVANMAGELFKVIVPAVPFVDVVTTMLDTSIPLTTL 603

Query: 653 EWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRAT 712
           EW+EWGDPRK+E YFYMKSYSP DNV+A++YP + +T G+NDPRV Y EPAK+VA+LRA 
Sbjct: 604 EWDEWGDPRKQEDYFYMKSYSPYDNVEAKDYPHMYITTGINDPRVGYFEPAKWVARLRAV 663

Query: 713 KTDNNLLLFKCELGAGHFSKSGRFEKLQEDAFTYAFILKSLNM 752
           KTDNN L+ K  +GAGHF KSGRF  L+E A +YAFIL  L +
Sbjct: 664 KTDNNTLVMKTNMGAGHFGKSGRFNHLKEAAESYAFILDKLGV 680

BLAST of Chy10G178620 vs. ExPASy Swiss-Prot
Match: O07834 (Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE=1 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.3e-145
Identity = 289/718 (40.25%), Postives = 425/718 (59.19%), Query Frame = 0

Query: 44  ATMSQSHSPP-VANKVENKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMT 103
           + ++ S +PP VA K        G  R D YYWLRDD R N ++L+YL  ENAYTD+VM 
Sbjct: 20  SALAASATPPDVAKKPHVVKAPHGAERNDEYYWLRDDKRENKEMLAYLNAENAYTDAVMA 79

Query: 104 GTKKVEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAIS 163
             K +ED+++ E+  RIK+DD +VP R+  ++YY R + GK+Y  + RR    G  +A+S
Sbjct: 80  PLKPLEDKLYDEVVARIKQDDASVPYRERGWWYYARFVTGKDYPVHARRKDGPG-VDAVS 139

Query: 164 VHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYI 223
           +      G  A  E V+LD N     + YY++G +EVS +N+L+AYA+DT G   YT+  
Sbjct: 140 IQAANAAGDFA-GEQVLLDVNALGAGKDYYNVGDYEVSQDNRLLAYADDTNGRRQYTIRF 199

Query: 224 IDAETGAPVGKPLVGVTSYLKWAGDD-ALVYITMD-EILRPDKAWLHKLGTEQSMDTCLY 283
            + +TG  +   +      L W+ D   L Y+  D E L   +   H LGT  S D  +Y
Sbjct: 200 KNLDTGELLPDTVTNAEPNLVWSDDGRTLFYVDKDPETLLSKRVKAHVLGTPASQDALVY 259

Query: 284 HEKDDMFSLDLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSH 343
            E+DD F + +  S   K++ I+ ES  +        + P     VL PR   ++    H
Sbjct: 260 EEEDDSFYMGIGRSRDDKFICISVESTVSSEMRCTPAASP-GVFTVLAPRERDVEYQADH 319

Query: 344 RGNHFFILRRSEEIFNSEVVACPLDNTSAT--TVILPHRESVKIQEIELFLNHIVILERE 403
            G+ + I   ++   N ++V  P D+TS       + HR+ V ++  ELF    V+ ER 
Sbjct: 320 LGDRWVIRTNADGATNFKIVTAPTDSTSRKDWKDWVAHRDDVFVEGFELFDGFSVVAERA 379

Query: 404 DGLPKVVVYSLPDIGEPLKTLEGGRAVDFA---DATYSVDV-LESEFSSSILRFCYSSMK 463
           + L            E L+ ++   + D+    ++ YS+ +    E  +  LR+ Y+SM 
Sbjct: 380 NAL------------ESLRVIKADGSSDYVKADESAYSMGLSANPETGTDWLRYSYTSMT 439

Query: 464 TPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWATALDG-TKVPLSIVYRKDLVK 523
           TP +TY+ + KTG     K + V  G+D++KYVTER WA A DG TK+P+++VYRKD+ +
Sbjct: 440 TPATTYEINTKTGERRQLKQQPV-PGYDASKYVTERVWAPARDGKTKIPVTLVYRKDVAR 499

Query: 524 LDGSDPLLLYGYGSYEYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLK 583
            DG  P+L Y YGSY   +DP+F  + +SLLDRG +Y +AHIRGG EMGR WY++GKL  
Sbjct: 500 -DGKAPMLQYAYGSYGASMDPNFSITNVSLLDRGVVYALAHIRGGQEMGRAWYDDGKLYN 559

Query: 584 KKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVD 643
           K NTFTDFI   +YL++  Y +K+++   G SAGGLL+GAV NM P+ +K  +  VPFVD
Sbjct: 560 KINTFTDFIDVTDYLVKEGYAAKDRVAAMGGSAGGLLMGAVSNMAPEKYKVILTLVPFVD 619

Query: 644 VLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRV 703
           V+TTMLDPTIPLTT+E++EWG+P ++ +Y Y+ +YSP DN++A+ YP + V  GL D +V
Sbjct: 620 VVTTMLDPTIPLTTNEYDEWGNPEEKGYYDYILTYSPYDNLQAKAYPAMFVGTGLWDSQV 679

Query: 704 LYSEPAKFVAKLRATKTDNNLLLFKCELGAGHFSKSGRFEKLQEDAFTYAFILKSLNM 752
            Y EPAK+VA+LR   T    ++F+  + AGH  KSGRF + +E A  +AF+L  L +
Sbjct: 680 QYWEPAKYVARLRDLNTGKGPVVFRTNMEAGHGGKSGRFRQYRERAEMFAFMLDQLGV 720

BLAST of Chy10G178620 vs. ExPASy Swiss-Prot
Match: P24555 (Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2)

HSP 1 Score: 484.2 bits (1245), Expect = 2.7e-135
Identity = 278/697 (39.89%), Postives = 390/697 (55.95%), Query Frame = 0

Query: 53  PVANKVENKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIF 112
           P A ++ + M L GD RIDNYYWLRDD+RS P+VL YL++EN+Y   VM   + ++D+I 
Sbjct: 3   PKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRIL 62

Query: 113 SEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPN 172
            EI  RI + +++ P  K  Y Y      G EY  Y R+     E +             
Sbjct: 63  KEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEEWD------------- 122

Query: 173 APPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVG 232
                 +LD N +A +  +YS+G   ++P+N ++A AED      Y +   + ETG    
Sbjct: 123 --EWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWYP 182

Query: 233 KPLVGVTSYLKWAGDDALVYITMDE--ILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLD 292
           + L  V     WA D  + Y        L P + W H +GT  S D  +Y EKDD + + 
Sbjct: 183 ELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVS 242

Query: 293 LEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRR 352
           L  + SK Y+ I   S  T     LD    +    V  PR    + Y      H F LR 
Sbjct: 243 LHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHE-YSLDHYQHRFYLRS 302

Query: 353 SEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLP 412
           +    N  +    + +      ++P RE++ ++   LF + +V+ ER+ GL      SL 
Sbjct: 303 NRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGLT-----SLR 362

Query: 413 DIGEPLKTLEGGRAVDFADATYSVDV-LESEFSSSILRFCYSSMKTPPSTYDYDMKTGVS 472
            I    + + G   + F D  Y   +    E  ++ LR+ YSSM TP + ++ DM TG  
Sbjct: 363 QINRKTREVIG---IAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGER 422

Query: 473 VLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE 532
            + K +T + GF +  Y +E  W  A DG +VP+S+VY +   +  G +PLL+YGYGSY 
Sbjct: 423 RVLK-QTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFR-KGHNPLLVYGYGSYG 482

Query: 533 YCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLI 592
             ID  F  SR+SLLDRGF+Y I H+RGGGE+G+QWYE+GK LKKKNTF D++ + + L+
Sbjct: 483 ASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALL 542

Query: 593 ENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSE 652
           +  Y S       G SAGG+L+G  IN RP+LF   IA VPFVDV+TTMLD +IPLTT E
Sbjct: 543 KLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGE 602

Query: 653 WEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATK 712
           +EEWG+P+  ++Y YMKSYSP DNV AQ YP +LVT GL+D +V Y EPAK+VAKLR  K
Sbjct: 603 FEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELK 662

Query: 713 TDNNLLLFKCELGAGHFSKSGRFEKLQEDAFTYAFIL 747
           TD++LLL   ++ +GH  KSGRF+  +  A  YAF++
Sbjct: 663 TDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLV 673

BLAST of Chy10G178620 vs. ExPASy Swiss-Prot
Match: P55656 (Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01580 PE=3 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 1.9e-88
Identity = 226/725 (31.17%), Postives = 356/725 (49.10%), Query Frame = 0

Query: 47  SQSHSPPVANKVENKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKK 106
           ++S  PP+         L  DV ID+Y WLRD  R +PDVL+YL  EN Y D V +   +
Sbjct: 3   NKSLQPPLPRSERRIRVLHNDVTIDSYGWLRD--REDPDVLAYLEAENHYADEVTSYVAE 62

Query: 107 VEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDT 166
           ++  + +EI  R   D    P + G ++Y++++  G  +  + RR V  G          
Sbjct: 63  LKADLIAEIEKRDSCDGAPPPFQVGFFFYFQKSQSGLLHSAWWRRPVTGG---------- 122

Query: 167 MPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAE 226
                   PE ++ D N     + +YS+G  E S + + +A++ D  G+E Y + + D  
Sbjct: 123 --------PEELVFDPNTLPGAEVFYSLGALEPSDDGRYIAFSFDLIGNERYELRVRDMT 182

Query: 227 TGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDM 286
            G  + +        L WA D+  ++ T +   R     + +L  E      ++ E ++ 
Sbjct: 183 NGREIWRD-PSRAGRLVWAADNRTLFFTRERADRRQHDRVVRLDVETGRSEVVFEEVNER 242

Query: 287 FSLDLEASESKKYLFI------ASESKFTRFN---FYLDVSRPEDGLVVLTPRVDGIDTY 346
            +L +  S S  YLFI         S+  R     + L   RP D    +  R  G + Y
Sbjct: 243 LALVVRRSGSGAYLFIDVIITSDMSSRIQRAAAEVWCLPAERPTDMWRRILARELGHEIY 302

Query: 347 PSHRGNHFFILRRSEEIFNSEVVACPLDNTSAT--TVILPHRESVKIQEIELFLNHIVIL 406
             H GN  F+ R ++   N  +V   +D+TS +    ++PHR  + ++EI +   H+++L
Sbjct: 303 AEHWGNE-FLFRVNDTGPNLRLVRTAIDDTSPSRWQEVVPHRAGITLEEIHVLEEHVIVL 362

Query: 407 EREDGLPKVVVYSLPD-IGEPLKTLEGGRAVDFA-DATYSVDVLESEFSSSILRFCYSSM 466
           ERE   P++V +     +G  +  +E    V     A  S       +  S L +   S 
Sbjct: 363 EREGIQPRLVAHHRNGRVGPSIVPVEHSCTVTVGLSAGGSYSCARHPYRVSALTYKICSF 422

Query: 467 KTPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVK 526
            TP     +D+ T  S +    T++ GF+   Y      A A DG +VP+SIV R+D   
Sbjct: 423 VTPDIFIQHDLLTDKSKV-LYRTLVSGFEPELYEARVVMAKAEDGVEVPISIVARRD--- 482

Query: 527 LDGSD-PLLLYGYGSY------EYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWY 586
             G D P+LL  YG Y       +   PS   +R+SLLDRG  + I H+RGGGE+GR W+
Sbjct: 483 -RGEDGPVLLNVYGCYGAQSLPAFFGWPSSMTARLSLLDRGVAFGIVHVRGGGELGRAWH 542

Query: 587 ENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAI 646
           E     +K+ T TD I++AE L+E+++ S++ + I GRSAGG  + A   +RPDLF+A +
Sbjct: 543 EAATRDQKRLTHTDLIAAAECLVEHRFASRDGIVIEGRSAGGGTVLAAAVLRPDLFRAVL 602

Query: 647 AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKA-QNYPDVLVT 706
           A VP  D++ T LD T+P    E  E+GDP     Y Y++SY P  N+   + YP   + 
Sbjct: 603 AEVPLADIIDTELDFTLPYALRETAEYGDPHLANDYQYLRSYDPYYNLTPDRRYPPTYID 662

Query: 707 AGLNDPRVLYSEPAKFVAKLRATKTDNNL-LLFKCELGAGHFSKSGRFEKLQEDAFTYAF 750
           A L+D +VLY +PA++VA+ R+   D +  L+F+  +  GH   S      +E AF  A+
Sbjct: 663 AALHDSQVLYYQPARYVAQRRSKAVDRDPDLIFRTRMIGGHMGVSHGPGVAEEAAFRMAW 700

BLAST of Chy10G178620 vs. ExPASy Swiss-Prot
Match: P55627 (Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01920 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.7e-82
Identity = 225/743 (30.28%), Postives = 360/743 (48.45%), Query Frame = 0

Query: 38  HFRSPVATMSQSHSPPVANKVENKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYT 97
           HFRS V    +S  PP+         L  DV +D Y WLRD  R NPDV +YL  EN+Y 
Sbjct: 26  HFRS-VVMWDKSLRPPLPRAEPRIRVLHDDVTVDRYGWLRD--RENPDVRAYLEAENSYA 85

Query: 98  DSVMTGTKKVEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGE 157
           +      ++++ ++ +EI GR   +  T P + G + Y++    G  +  + RR V  G 
Sbjct: 86  EQATAHLRRLKTELIAEIEGRQPCEGATPPFQVGPFDYFQGHERGLPHPVWWRRPVTGGS 145

Query: 158 EEAISVHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEI 217
            E                  ++LD N       +Y +G FE S + + +A++ D  G E 
Sbjct: 146 AE------------------LVLDPNAIPGADVFYWLGVFEPSDDGRYLAFSVDLIGAER 205

Query: 218 YTVYIIDAETGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLH---KLGTEQS 277
           Y + + D   G  V +   G    + WA D+  ++ T +   RPD+   H   +L   + 
Sbjct: 206 YELRVRDMSDGRDVWRD-AGSVGQVVWAADNHTLFFTRE---RPDRRQHHQIVRLNVGRG 265

Query: 278 MDTCLYHEKDDMFSLDLEASESKKYLFI-----ASESKFTRFN----FYLDVSRPEDGLV 337
               ++ E ++  ++ +  S+S  +LF+     +  S + +      + L    P     
Sbjct: 266 NSEVVFEEANERLAVLVRRSQSGAWLFLDVLTTSDMSSYVQRGAAEVWCLPADEPGGQWR 325

Query: 338 VLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVVACPLDNTSAT--TVILPHRESVKIQ 397
            +  R  G   Y  H  +  F+ R  +      +V+ P+D+ S +    ++PHR  V I 
Sbjct: 326 RIVMRELGHQIYAEHWYDR-FLFRVDDAGPYWRLVSAPIDDPSPSRWEEVVPHRAGVTID 385

Query: 398 EIELFLNHIVILEREDGLPKVV---------VYSLPDIGEPLKTLEGGRAVDFADATYSV 457
           EI +   H+V+LERE   P+++            +PD  EP  T+  G +   A   YS 
Sbjct: 386 EIHVLEQHLVLLEREGLRPRLISRNRSGRVGAVIVPD--EPSCTIRVGLS---AGGCYS- 445

Query: 458 DVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWAT 517
                 F SS L +  SS  TP +  ++D     SV+   E  + G+D+ +Y+     A 
Sbjct: 446 -AARHPFRSSKLTYSVSSFVTPDTFIEHDFANDRSVV-LCEARVPGYDATQYLATVVMAE 505

Query: 518 ALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKA------SRISLLDRGF 577
           A DG +VP+S+V R+D        P+LL  YG Y     PSF A      +R+SLLDR  
Sbjct: 506 AEDGVQVPISLVARRDRT---SPGPVLLSVYGCYGIPRLPSFLAWPSSMTARLSLLDREV 565

Query: 578 IYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGG 637
            + I H+RGGGE+GR W++     +K+ T TD IS+ E LIE  + +++ + I G+S GG
Sbjct: 566 AFGIVHVRGGGELGRPWHDAATRDQKRITHTDLISATEGLIERGFATRDGVVIEGKSGGG 625

Query: 638 LLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSY 697
             + A    RP+LF+A +A VP  D++ T LD T+P T  E  E+GDP+    Y Y++SY
Sbjct: 626 GTVLATAVFRPNLFRAVVAEVPLADIIDTQLDSTMPYTLKETAEYGDPQDAYEYRYLRSY 685

Query: 698 SPVDNVKAQ-NYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNL-LLFKCELGAGHF 750
            P  N+  + + P   V A L+D +V+Y +PA++VA+ R+  TD +  L+F+  +  GH 
Sbjct: 686 DPYYNLSPERSLPPTYVDAALDDGQVIYYQPARYVAQRRSCATDRDPDLVFRIRMVGGHS 731

BLAST of Chy10G178620 vs. ExPASy TrEMBL
Match: A0A0A0KV30 (Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G579570 PE=3 SV=1)

HSP 1 Score: 1524.6 bits (3946), Expect = 0.0e+00
Identity = 747/757 (98.68%), Postives = 751/757 (99.21%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           MTPLQQNGIFGILRRSFV  IPVLYLSP LPS ASFRHFRSPVATM+QSHSPPVANKVE+
Sbjct: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREEN YTDSVM+GTKKVEDQIFSEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVD LESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQNYPDVL+TAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 758
           LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 757

BLAST of Chy10G178620 vs. ExPASy TrEMBL
Match: A0A5A7STX7 (Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003340 PE=3 SV=1)

HSP 1 Score: 1491.9 bits (3861), Expect = 0.0e+00
Identity = 728/757 (96.17%), Postives = 743/757 (98.15%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           MT LQQN IFGILRRSF+  IPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVE+
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR NPDVLSYL++ENAYTDSVM+GTKKVE QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDD+TVPER+GSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDN SATTVILPHRESVKIQ+I+LFLNHIVI EREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVD  ESEFSSSILRFCYSSMKTP STYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSFKPSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQNYPD+LVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 758
           LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPA+GN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPALGN 757

BLAST of Chy10G178620 vs. ExPASy TrEMBL
Match: A0A1S3BDS7 (Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103488786 PE=3 SV=1)

HSP 1 Score: 1491.9 bits (3861), Expect = 0.0e+00
Identity = 728/757 (96.17%), Postives = 743/757 (98.15%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           MT LQQN IFGILRRSF+  IPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVE+
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR NPDVLSYL++ENAYTDSVM+GTKKVE QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDD+TVPER+GSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDN SATTVILPHRESVKIQ+I+LFLNHIVI EREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVD  ESEFSSSILRFCYSSMKTP STYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSFKPSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQNYPD+LVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 758
           LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPA+GN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPALGN 757

BLAST of Chy10G178620 vs. ExPASy TrEMBL
Match: A0A6J1G021 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111449474 PE=3 SV=1)

HSP 1 Score: 1401.3 bits (3626), Expect = 0.0e+00
Identity = 676/757 (89.30%), Postives = 720/757 (95.11%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           M PLQQNGIFG++RRS V  +PV+ LSP  PSVAS RHFRS  ATMS SHSPPVA KVE+
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSAAATMSNSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++ENAYT+ VM+GTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTELVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKL TEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLETEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHFFI RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPLK+LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLKSLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV + +SEFSSSILRFCYSSM+TPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKL+KKKNTFTDFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLMKKKNTFTDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQ+YPD+LVTAGLNDPRVLYSEPAKFVAKLR  KTD+NLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQDYPDILVTAGLNDPRVLYSEPAKFVAKLRDMKTDHNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 758
           LGAGHFSKSGRFEKLQEDAFTYAFILKS+NMIPA+GN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSVNMIPALGN 756

BLAST of Chy10G178620 vs. ExPASy TrEMBL
Match: A0A6J1HU74 (Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111466641 PE=3 SV=1)

HSP 1 Score: 1397.9 bits (3617), Expect = 0.0e+00
Identity = 674/757 (89.04%), Postives = 718/757 (94.85%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           M PLQQNGIFG++RRS V  +PV+ LSP  P VAS RHFRS  ATMS SHSPPVA KVE+
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPLVASLRHFRSAAATMSNSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++ENAYTD VM+GTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDLVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHF I RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFLIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPL++LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV + +SEFSS+ILRFCYSSM+TPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSTILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD   YVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITNYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQ+YPD+LVTAGLNDPRVLYSEPAKFVAKLR  KTD+NLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQDYPDILVTAGLNDPRVLYSEPAKFVAKLRDMKTDHNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 758
           LGAGHFSKSGRFEKLQEDAFTYAFILKS+NMIPA+GN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSVNMIPALGN 756

BLAST of Chy10G178620 vs. NCBI nr
Match: XP_004147101.1 (uncharacterized protein LOC101213609 [Cucumis sativus] >KGN51571.1 hypothetical protein Csa_008158 [Cucumis sativus])

HSP 1 Score: 1514 bits (3920), Expect = 0.0
Identity = 747/757 (98.68%), Postives = 751/757 (99.21%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           MTPLQQNGIFGILRRSFV  IPVLYLSP LPS ASFRHFRSPVATM+QSHSPPVANKVE+
Sbjct: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREEN YTDSVM+GTKKVEDQIFSEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVD LESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQNYPDVL+TAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 757
           LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 757

BLAST of Chy10G178620 vs. NCBI nr
Match: XP_008445904.1 (PREDICTED: protease 2 [Cucumis melo] >KAA0034088.1 protease 2 [Cucumis melo var. makuwa] >TYK15829.1 protease 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1481 bits (3835), Expect = 0.0
Identity = 728/757 (96.17%), Postives = 743/757 (98.15%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           MT LQQN IFGILRRSF+  IPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVE+
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR NPDVLSYL++ENAYTDSVM+GTKKVE QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDD+TVPER+GSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDN SATTVILPHRESVKIQ+I+LFLNHIVI EREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVD  ESEFSSSILRFCYSSMKTP STYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSFKPSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQNYPD+LVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 757
           LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPA+GN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPALGN 757

BLAST of Chy10G178620 vs. NCBI nr
Match: XP_038893092.1 (protease 2 [Benincasa hispida])

HSP 1 Score: 1437 bits (3720), Expect = 0.0
Identity = 697/757 (92.07%), Postives = 733/757 (96.83%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           M PLQQN IFG++RRS + LIPV+YLSPALPSVASFRHFRSPVATMSQSHSPPVANKVE+
Sbjct: 1   MKPLQQNSIFGLVRRSLILLIPVVYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++ENAYTD VM+GTK+VE+QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDFVMSGTKQVEEQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKG YYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGP+APPEHVIL
Sbjct: 121 EDDISVPERKGPYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPDAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPL GVTS
Sbjct: 181 DENVKAQNQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLAGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAG+DALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGNDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVS+PEDGLVVLTPRVDG+DT+PSHRGNHFFI RRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSKPEDGLVVLTPRVDGVDTFPSHRGNHFFIRRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSATTVILPHRESVKIQ+I+LFLN I+I EREDGLPK+VVYSLPDIGEPL++LE
Sbjct: 361 ACPLDNTSATTVILPHRESVKIQDIQLFLNQIIIFEREDGLPKIVVYSLPDIGEPLRSLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSVD  ESEFSSS+LRFCYSSMKTPPSTYDYDMKTGVS+LKKVETVLGG
Sbjct: 421 GGRAVDFTDATYSVDASESEFSSSVLRFCYSSMKTPPSTYDYDMKTGVSILKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD+NKYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFKASR
Sbjct: 481 FDTNKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEVCVDPSFKASR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIY IAHIRGGGEMGRQWYENGKLLKKKNTFTDFIS AEYLIENKY SKEKLC
Sbjct: 541 ISLLDRGFIYAIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYGSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIG+V+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGSVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQNYPD+LVTAGLNDPRVLYSEPAKFVAKLR  KTD+NLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLNDPRVLYSEPAKFVAKLRDMKTDDNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 757
           LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIP +GN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPTLGN 757

BLAST of Chy10G178620 vs. NCBI nr
Match: XP_023541732.1 (uncharacterized protein LOC111801801 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1397 bits (3617), Expect = 0.0
Identity = 680/757 (89.83%), Postives = 720/757 (95.11%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           M PLQQNGIFG++RRS V  +PV+ LSP  PSVAS RHFRS  ATMS SHSPPVA KVE+
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSAAATMSNSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++ENAYTD VM+GTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDLVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHFFI RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPL++LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV + +SEFSSSILRFCYSSMKTPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMKTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTF DFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFMDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQNYPD+LVTAGLNDPRVLYSEPAKFVAKLR  KTD+NLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLNDPRVLYSEPAKFVAKLRDMKTDHNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 757
           LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPA+GN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPALGN 756

BLAST of Chy10G178620 vs. NCBI nr
Match: KAG6573758.1 (Protease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1393 bits (3605), Expect = 0.0
Identity = 676/757 (89.30%), Postives = 720/757 (95.11%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVFLIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEN 60
           M PLQQNGIFG++RRS V  +PV+ LSP  PSVAS RHFRS  ATMS SHSPPV  KVE+
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSTAATMSNSHSPPVVKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++ENAYT+ VM+GTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTELVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHFFI RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPLK+LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLKSLE 420

Query: 421 GGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV + +SEFSSSILRFCYSSM+TPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKL+KKKNTFTDFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLMKKKNTFTDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 720
           FYFYMKSYSPVDNVKAQ+YPD+LVTAGLNDPRVLYSEPAKFVAKLR  KTD+NLLLFKCE
Sbjct: 661 FYFYMKSYSPVDNVKAQDYPDILVTAGLNDPRVLYSEPAKFVAKLRDMKTDHNLLLFKCE 720

Query: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN 757
           LGAGHFSKSGRFEKLQEDAFTYAFILKS+NMIPA+GN
Sbjct: 721 LGAGHFSKSGRFEKLQEDAFTYAFILKSVNMIPALGN 756

BLAST of Chy10G178620 vs. TAIR 10
Match: AT1G50380.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 540/711 (75.95%), Postives = 620/711 (87.20%), Query Frame = 0

Query: 46  MSQSHSPPVANKVENKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENAYTDSVMTGTK 105
           M++S SPPVA KVE+ ME+FGDVR+DNYYWLRDDSR+NPD+LSYLREEN YTD VM+GTK
Sbjct: 1   MAESRSPPVAKKVEHVMEMFGDVRVDNYYWLRDDSRTNPDMLSYLREENHYTDFVMSGTK 60

Query: 106 KVEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHD 165
           + E+Q+F+EIRGRIKEDDI+ P RKG YYYYE+ L+GKEY+Q+CRR +   + E  SV+D
Sbjct: 61  QFENQLFAEIRGRIKEDDISAPLRKGPYYYYEKNLQGKEYIQHCRRLITDNKAEP-SVYD 120

Query: 166 TMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDA 225
           TMPTGP+APPEHVILDEN KA+   YY IG F+ SP++KLVAYAEDTKGDEIYTV +ID+
Sbjct: 121 TMPTGPDAPPEHVILDENTKAQEHDYYRIGAFKASPDHKLVAYAEDTKGDEIYTVNVIDS 180

Query: 226 ETGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDD 285
           E   PVG+ L G+TSYL+WAG+DAL+YITMDEILRPDK WLHKLGTEQS D CLYHEKDD
Sbjct: 181 EALKPVGQQLKGLTSYLEWAGNDALLYITMDEILRPDKVWLHKLGTEQSSDVCLYHEKDD 240

Query: 286 MFSLDLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHF 345
           MFSL+L ASES KYLF+ASESK TRF F LDVS+ +DGL VLTPRVDGID+  SHRGNHF
Sbjct: 241 MFSLELHASESHKYLFVASESKTTRFVFSLDVSKTQDGLRVLTPRVDGIDSSVSHRGNHF 300

Query: 346 FILRRSEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVV 405
           FI RRS E +NSE++ACP+D+TS TTV+LPHRESVKIQEI+LF +H+ + ERE+GL K+ 
Sbjct: 301 FIQRRSTEFYNSELIACPVDDTSKTTVLLPHRESVKIQEIQLFRDHLAVFERENGLQKIT 360

Query: 406 VYSLPDIGEPLKTLEGGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYDYDMK 465
           V+ LP  G+PL+ L+GGR V F D  YSVD  ESEFSS +LRF Y SMKTPPS YDYDM 
Sbjct: 361 VHRLPAEGQPLEGLQGGRNVSFVDPVYSVDSTESEFSSRVLRFKYCSMKTPPSVYDYDMD 420

Query: 466 TGVSVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGY 525
           +G SV+KK++TVLGGFD++ YVTERKW  A DGT++P+SIVY K L KLDGSDPLLLYGY
Sbjct: 421 SGTSVVKKIDTVLGGFDASNYVTERKWVAASDGTQIPMSIVYNKKLAKLDGSDPLLLYGY 480

Query: 526 GSYEYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSA 585
           GSYE  +DP FKASR+SLLDRGF +VIAH+RGGGEMGRQWYENGKLLKKKNTFTDFI+ A
Sbjct: 481 GSYEISVDPYFKASRLSLLDRGFTFVIAHVRGGGEMGRQWYENGKLLKKKNTFTDFIACA 540

Query: 586 EYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPL 645
           E LIE KYCSKEKLC+ GRSAGGLL+GAV+NMRPDLFK  IAGVPFVDVLTTMLDPTIPL
Sbjct: 541 ERLIELKYCSKEKLCMEGRSAGGLLMGAVVNMRPDLFKVVIAGVPFVDVLTTMLDPTIPL 600

Query: 646 TTSEWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLVTAGLNDPRVLYSEPAKFVAKL 705
           TTSEWEEWGDPRKEEFYFYMKSYSPVDNV AQNYP++LVTAGLNDPRV+YSEP K+VAKL
Sbjct: 601 TTSEWEEWGDPRKEEFYFYMKSYSPVDNVTAQNYPNMLVTAGLNDPRVMYSEPGKWVAKL 660

Query: 706 RATKTDNNLLLFKCELGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVG 757
           R  KTDNN+LLFKCELGAGHFSKSGRFEKLQEDAFT+AF++K L+MIPA G
Sbjct: 661 REMKTDNNVLLFKCELGAGHFSKSGRFEKLQEDAFTFAFMMKVLDMIPASG 710

BLAST of Chy10G178620 vs. TAIR 10
Match: AT1G69020.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 332.4 bits (851), Expect = 9.4e-91
Identity = 238/783 (30.40%), Postives = 382/783 (48.79%), Query Frame = 0

Query: 25  YLSPALPSVASF--RHFRSPVATMS-QSHSPPVANKVENKMELFGDVRIDNYYWLRDDSR 84
           Y    + SV SF  + F    +++S  + +PPV  K+   +   G  R D ++W++  + 
Sbjct: 10  YKPATISSVLSFSTKCFVGRTSSLSVPTEAPPVPKKIPFAISSHGITRQDPFHWMK--NT 69

Query: 85  SNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLE 144
            + D + +L+ EN+Y+ + M  T+ +   +FSE++ RI E+  T PER G + Y +   +
Sbjct: 70  DDTDFVDFLKRENSYSQAFMADTETLRRDLFSEMKTRIPEEIFTPPERWGQWLYRQYIPK 129

Query: 145 GKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSP 204
           GKEY   CRR + +G+   +S             E V+LD N  AE   Y  +G   VSP
Sbjct: 130 GKEYPLLCRR-LEKGKTNWLSGLF------RGEEEEVVLDWNQIAEQFGYVHVGVCRVSP 189

Query: 205 NNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRP 264
           ++  +AY  D +GD                     G+T          L Y   DE  RP
Sbjct: 190 DHNYLAYTVDPEGD---------------------GIT----------LFYTVTDENQRP 249

Query: 265 DKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYLFIASESKFTRFNFYLDVSRPE 324
            +  +  + ++   D  ++ E+D  F +D+  ++  K++ I S S+ +   + ++  +P 
Sbjct: 250 HRVVVTNVESDGRDDAVVFTERDSSFCVDITTTKDGKFVTINSNSRTSSEVYIVNADKPM 309

Query: 325 DGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSE-------VVACPLDNTSAT---T 384
            GL     RV G+  +  H    F+IL  S     SE       +  C ++   A+   T
Sbjct: 310 AGLQRTRERVPGVQCFLEHHNGFFYILTNSPSNAISEWSGEGYYLTRCLVEEIEASDWQT 369

Query: 385 VILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLEGGRAVDFADAT 444
           V  P  + V IQ++++F +++V+   + GLP +    +P I    K ++      F    
Sbjct: 370 VFRPD-DDVVIQDMDMFNDYLVLYLNKKGLPMLCSIDMP-IKANTKHMDDLVPWYFPLPV 429

Query: 445 YSVDVL---ESEFSSSILRFCYSSMKTPPSTYDYDMKTGV-SVLKKVETVLGGFDSNK-- 504
            S  V      +F SSI R   SS   P +  DYD+   + S++++   V+   DS+K  
Sbjct: 430 DSCSVAPGSNHDFQSSIYRVVLSSPVIPDTIVDYDVSRRLFSIVQQEGGVVDNSDSSKPW 489

Query: 505 --------------------------------------YVTERKWATALDGTKVPLSIVY 564
                                                 YV ER+  ++ DG +VPL+I+Y
Sbjct: 490 YTADRSTENNGQLNDRTSEGEDGQLDSRMPKWEDLSDTYVCERQEVSSHDGVEVPLTILY 549

Query: 565 RKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYE 624
            ++  K   S P +L GYG+Y   +D S+  +R+S+LDRG++   A +RGGG     W++
Sbjct: 550 SREAWKKSES-PGMLIGYGAYGEVLDKSWCTNRLSMLDRGWVIAFADVRGGGSGEFSWHK 609

Query: 625 NGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIA 684
           +G    K+N+  DFI SA+YL+E  Y  +  L   G SAG +L  A +NM P LF+A I 
Sbjct: 610 SGTRSLKQNSIQDFIYSAKYLVEKGYVHRHHLAAVGYSAGAILPAAAMNMHPSLFQAVIL 669

Query: 685 GVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNV-KAQNYPDVLVTA 744
            VPFVDVL T+ DP +PLT  + EE+G+P  +  +  + SYSP D + K   YP +LVT 
Sbjct: 670 KVPFVDVLNTLSDPNLPLTLLDHEEFGNPDNQTDFGSILSYSPYDKIRKDVCYPSMLVTT 729

Query: 745 GLNDPRVLYSEPAKFVAKLR-ATKTD-NNLLLFKCELGAGHFSKSGRFEKLQEDAFTYAF 748
             +D RV   E AK+VAK+R +T  D +  ++ K  +  GHF + GR+ + +E AF YAF
Sbjct: 730 SFHDSRVGVWEGAKWVAKIRDSTCHDCSRAVILKTNMNGGHFGEGGRYAQCEETAFDYAF 749

BLAST of Chy10G178620 vs. TAIR 10
Match: AT5G66960.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 252.7 bits (644), Expect = 9.5e-67
Identity = 205/749 (27.37%), Postives = 347/749 (46.33%), Query Frame = 0

Query: 71  DNYYWLR--DDSRSNPDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIKEDDITVPE 130
           D Y W+   +D  +   +  Y+ +E  YT++V+  T +++ ++ SE+  R+  +  T P 
Sbjct: 58  DPYSWMSKLEDKVAMRHMDIYMEQEEKYTEAVLADTDRIQTKLQSEMASRLSFELSTPPL 117

Query: 131 RKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTG----PNAPPEHVILDENV 190
           R G + YY R  EGK+Y   CRR      EE IS H +   G         E  +LD N 
Sbjct: 118 RWGPWLYYRRVEEGKQYPVLCRRLASL-HEEFIS-HKSPAAGFDYTSGKRIEQKLLDYNQ 177

Query: 191 KAENQSYYSI-GCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTSYLK 250
           +AE    Y+     E+SP++K +AY    K ++ + + + +  +GA   KP     S + 
Sbjct: 178 EAERFGGYAYEEMSEISPDHKFLAYTMYDKDNDYFKLCVRNLNSGALCSKPHADRVSNIA 237

Query: 251 WA-GDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYLFI 310
           WA    AL+Y+  D+  RP + +   +G+    D  L+ E +   ++ +    +K + F+
Sbjct: 238 WAKNGQALLYVVTDQKKRPCRIYCSTIGSTDE-DVLLHEEFEG--NVHVNIRHTKDFHFV 297

Query: 311 ASESKFTRFN--FYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEE----IFN 370
              +  T F+  F ++ + P  GL ++            H     ++   +      + +
Sbjct: 298 TVNTFSTTFSKVFLINAADPFSGLALVWEHNAPAHCIIEHHQGFLYLFTNASNDGGTVDH 357

Query: 371 SEVVACPLDNTSA----TTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLP-- 430
             ++  P+  +S      TV +   E + I++++    H+ ++ +E    K+ V  LP  
Sbjct: 358 HYLLRSPVHFSSCQRIWETVFINDPELI-IEDVDFCKKHLSLIVKEMQSFKICVVDLPLK 417

Query: 431 ---------DIGEPLKTLEGGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPPSTYD 490
                    DI      L    +  F    Y       +F+S  +RF  SS+  P +  D
Sbjct: 418 TKRVPVHLRDIKPRYLPLPKHVSQIFPGTNY-------DFNSPTMRFTISSLVMPDAVVD 477

Query: 491 YDMKTG-------VSVLKKVETVLGG------------------FDSNK----------- 550
           YD+  G        ++L +   VL G                  FD+             
Sbjct: 478 YDLLNGKWNIVQQQNMLHERTRVLYGTANSTESPNIPSGTRTVSFDTEDTTAENDNLWND 537

Query: 551 ----YVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASRI 610
               Y  +    ++ DG  VPLSIVY +   K +   P LL+ +G+Y   +D  +++   
Sbjct: 538 LTEFYACDYHEVSSHDGAMVPLSIVYSR-AQKEENQKPGLLHVHGAYGEMLDKRWRSELK 597

Query: 611 SLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCI 670
           SLLDRG++   A +RGGG  G++W+++G+  KK N+  D+I  A+YL+EN    + KL  
Sbjct: 598 SLLDRGWVLAYADVRGGGGKGKKWHQDGRGAKKLNSIKDYIQCAKYLVENNIVEENKLAG 657

Query: 671 NGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF 730
            G SAGGL++ + IN  PDLF+AA+  VPF+D   T++ P +PLT  ++EE+G P     
Sbjct: 658 WGYSAGGLVVASAINHCPDLFQAAVLKVPFLDPTHTLIYPILPLTAEDYEEFGYPGDIND 717

Query: 731 YFYMKSYSPVDNV-KAQNYPDVLVTAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCE 750
           +  ++ YSP DN+ K   YP VLVT+  N  R    E AK+VA++R    ++        
Sbjct: 718 FHAIREYSPYDNIPKDVLYPAVLVTSSFN-TRFGVWEAAKWVARVRDNTFNDPERPVLLN 777

BLAST of Chy10G178620 vs. TAIR 10
Match: AT1G76140.2 (Prolyl oligopeptidase family protein )

HSP 1 Score: 226.1 bits (575), Expect = 9.5e-59
Identity = 213/788 (27.03%), Postives = 339/788 (43.02%), Query Frame = 0

Query: 31  PSVASFRHFRSPVATMSQSH------SPPVANKVENKMELFGDVRI-DNYYWLRDDSRSN 90
           P+  S R + S  A M  S         P   + ++ ++ +  V+I D Y WL D     
Sbjct: 50  PNSVSRRCYCSSSAIMGSSSVFGEQLQYPATRRDDSVVDDYHGVKIGDPYRWLEDPDAE- 109

Query: 91  PDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIKEDDITVPERKG-SYYYYERTLEG 150
            +V  +++ +   TDSV+    + ++++   I   I       P R+G  Y+Y+  T   
Sbjct: 110 -EVKEFVQSQVKLTDSVLEKC-ETKEKLRQNITKLIDHPRYDSPFRQGDKYFYFHNTGLQ 169

Query: 151 KEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPN 210
            + V Y                  M    +A PE V+LD N  +++ +  ++  F VS +
Sbjct: 170 AQSVLY------------------MQDNLDAEPE-VLLDPNTLSDDGT-VALNTFSVSED 229

Query: 211 NKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVT-SYLKWAGDDALVYI-------- 270
            K +AY   + G +  T+ ++  E        L  V  + + W  D    +         
Sbjct: 230 AKYLAYGLSSSGSDWVTIKLMKIEDKKVEPDTLSWVKFTGITWTHDSKGFFYGRYPAPKE 289

Query: 271 -------TMDEILRPDKAWLHKLGTEQSMD-TCLYHEKDDMFSLDLEASESKKYLFIASE 330
                  T        + + H +GT+QS D  C    ++  +    E ++  KYL ++  
Sbjct: 290 GEDIDAGTETNSNLYHELYYHFIGTDQSQDILCWRDNENPKYMFGAEVTDDGKYLIMSIG 349

Query: 331 SKFTRFN--FYLDVSRPEDGL------VVLTPRVDGIDTYPSHRGNHFFILRRSEEIF-- 390
                 N  +Y D++    GL          P +  +DT+ +     + ++   E +F  
Sbjct: 350 ESCDPVNKLYYCDMTSLSGGLESFRGSSSFLPFIKLVDTFDA----QYSVISNDETLFTF 409

Query: 391 --NSEVVACPL------DNTSATTVILPHRESVKIQEIELFLNHIV---------ILERE 450
             N +     L      +  S T V+  H + V      +  NH+V         IL+  
Sbjct: 410 LTNKDAPKYKLVRVDLKEPNSWTDVVEEHEKDVLASACAVNGNHLVACYMSDVKHILQIR 469

Query: 451 DGLPKVVVYSLP-DIGEPLKTLEGGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPP 510
           D     +++ LP DIG                   SV  + +    +   F ++S  TP 
Sbjct: 470 DLKSGSLLHQLPLDIG-------------------SVSDVSARRKDNTFFFSFTSFLTPG 529

Query: 511 STYDYDMKTGVSVLKKV-ETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDG 570
             Y  D+      +K   E  + GFD   +   + +  + DGTK+P+ IV +KD +KLDG
Sbjct: 530 VIYKCDLANESPEVKVFREVTVPGFDREAFQAIQVFYPSKDGTKIPMFIVAKKD-IKLDG 589

Query: 571 SDPLLLYGYGSYEYCIDPSFKASRISLLDR-GFIYVIAHIRGGGEMGRQWYENGKLLKKK 630
           S P LLY YG +   I PSF ASRI L    G ++  A+IRGGGE G +W++ G L KK+
Sbjct: 590 SHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFANIRGGGEYGEEWHKAGSLAKKQ 649

Query: 631 NTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVL 690
           N F DFIS AEYL+   Y    KLCI G S GGLL+GA IN RPDL+  A+A V  +D+L
Sbjct: 650 NCFDDFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDML 709

Query: 691 TTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKAQ---------NYPDVLVTA 750
                      TS   ++G    EE + ++  YSP+ NVK            YP  ++  
Sbjct: 710 RFHKFTIGHAWTS---DYGCSENEEEFHWLIKYSPLHNVKRPWEQQTDHLVQYPSTMLLT 769

BLAST of Chy10G178620 vs. TAIR 10
Match: AT1G76140.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 223.0 bits (567), Expect = 8.1e-58
Identity = 214/791 (27.05%), Postives = 340/791 (42.98%), Query Frame = 0

Query: 31  PSVASFRHFRSPVATMSQSH------SPPVANKVENKMELFGDVRI-DNYYWLRDDSRSN 90
           P+  S R + S  A M  S         P   + ++ ++ +  V+I D Y WL D     
Sbjct: 50  PNSVSRRCYCSSSAIMGSSSVFGEQLQYPATRRDDSVVDDYHGVKIGDPYRWLEDPDAE- 109

Query: 91  PDVLSYLREENAYTDSVMTGTKKVEDQIFSEIRGRIKEDDITVPERKG-SYYYYERTLEG 150
            +V  +++ +   TDSV+    + ++++   I   I       P R+G  Y+Y+  T   
Sbjct: 110 -EVKEFVQSQVKLTDSVLEKC-ETKEKLRQNITKLIDHPRYDSPFRQGDKYFYFHNTGLQ 169

Query: 151 KEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPN 210
            + V Y                  M    +A PE V+LD N  +++ +  ++  F VS +
Sbjct: 170 AQSVLY------------------MQDNLDAEPE-VLLDPNTLSDDGT-VALNTFSVSED 229

Query: 211 NKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVT-SYLKWAGDDALVYI-------- 270
            K +AY   + G +  T+ ++  E        L  V  + + W  D    +         
Sbjct: 230 AKYLAYGLSSSGSDWVTIKLMKIEDKKVEPDTLSWVKFTGITWTHDSKGFFYGRYPAPKE 289

Query: 271 -------TMDEILRPDKAWLHKLGTEQSMD-TCLYHEKDDMFSLDLEASESKKYLFIASE 330
                  T        + + H +GT+QS D  C    ++  +    E ++  KYL ++  
Sbjct: 290 GEDIDAGTETNSNLYHELYYHFIGTDQSQDILCWRDNENPKYMFGAEVTDDGKYLIMSIG 349

Query: 331 SKFTRFN--FYLDVSRPEDGL------VVLTPRVDGIDTYPSHRGNHFFILRRSEEIF-- 390
                 N  +Y D++    GL          P +  +DT+ +     + ++   E +F  
Sbjct: 350 ESCDPVNKLYYCDMTSLSGGLESFRGSSSFLPFIKLVDTFDA----QYSVISNDETLFTF 409

Query: 391 --NSEVVACPL------DNTSATTVILPHRESVKIQEIELFLNHIV---------ILERE 450
             N +     L      +  S T V+  H + V      +  NH+V         IL+  
Sbjct: 410 LTNKDAPKYKLVRVDLKEPNSWTDVVEEHEKDVLASACAVNGNHLVACYMSDVKHILQIR 469

Query: 451 DGLPKVVVYSLP-DIGEPLKTLEGGRAVDFADATYSVDVLESEFSSSILRFCYSSMKTPP 510
           D     +++ LP DIG                   SV  + +    +   F ++S  TP 
Sbjct: 470 DLKSGSLLHQLPLDIG-------------------SVSDVSARRKDNTFFFSFTSFLTPG 529

Query: 511 STYDYDMKTGVSVLKKV-ETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDG 570
             Y  D+      +K   E  + GFD   +   + +  + DGTK+P+ IV +KD +KLDG
Sbjct: 530 VIYKCDLANESPEVKVFREVTVPGFDREAFQAIQVFYPSKDGTKIPMFIVAKKD-IKLDG 589

Query: 571 SDPLLLYGYGSYEYCIDPSFKASRISLLDR-GFIYVIAHIRGGGEMGRQWYENGKLLKKK 630
           S P LLY YG +   I PSF ASRI L    G ++  A+IRGGGE G +W++ G L KK+
Sbjct: 590 SHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFANIRGGGEYGEEWHKAGSLAKKQ 649

Query: 631 NTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVL 690
           N F DFIS AEYL+   Y    KLCI G S GGLL+GA IN RPDL+  A+A V  +D+L
Sbjct: 650 NCFDDFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDML 709

Query: 691 TTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKAQ---------NYPDVLVTA 750
                      TS   ++G    EE + ++  YSP+ NVK            YP  ++  
Sbjct: 710 RFHKFTIGHAWTS---DYGCSENEEEFHWLIKYSPLHNVKRPWEQQTDHLVQYPSTMLLT 769

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q595362.3e-15040.40Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1[more]
O078341.3e-14540.25Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE... [more]
P245552.7e-13539.89Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2[more]
P556561.9e-8831.17Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
P556272.7e-8230.28Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
Match NameE-valueIdentityDescription
A0A0A0KV300.0e+0098.68Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G579570 PE=3 SV=1[more]
A0A5A7STX70.0e+0096.17Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G... [more]
A0A1S3BDS70.0e+0096.17Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103488786 PE=3 SV=1[more]
A0A6J1G0210.0e+0089.30Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111449474 PE=3 SV=1[more]
A0A6J1HU740.0e+0089.04Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111466641 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147101.10.098.68uncharacterized protein LOC101213609 [Cucumis sativus] >KGN51571.1 hypothetical ... [more]
XP_008445904.10.096.17PREDICTED: protease 2 [Cucumis melo] >KAA0034088.1 protease 2 [Cucumis melo var.... [more]
XP_038893092.10.092.07protease 2 [Benincasa hispida][more]
XP_023541732.10.089.83uncharacterized protein LOC111801801 [Cucurbita pepo subsp. pepo][more]
KAG6573758.10.089.30Protease 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G50380.10.0e+0075.95Prolyl oligopeptidase family protein [more]
AT1G69020.19.4e-9130.40Prolyl oligopeptidase family protein [more]
AT5G66960.19.5e-6727.37Prolyl oligopeptidase family protein [more]
AT1G76140.29.5e-5927.03Prolyl oligopeptidase family protein [more]
AT1G76140.18.1e-5827.05Prolyl oligopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002470Peptidase S9A, prolyl oligopeptidasePRINTSPR00862PROLIGOPTASEcoord: 661..676
score: 51.97
coord: 679..701
score: 45.08
coord: 603..623
score: 63.41
coord: 545..569
score: 56.63
coord: 573..592
score: 47.89
coord: 519..537
score: 47.09
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 534..750
e-value: 8.6E-68
score: 228.0
IPR023302Peptidase S9A, N-terminal domainPFAMPF02897Peptidase_S9_Ncoord: 52..473
e-value: 1.8E-103
score: 346.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 71..747
e-value: 1.9E-252
score: 841.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 486..731
NoneNo IPR availableGENE3D2.130.10.120coord: 122..476
e-value: 1.9E-252
score: 841.5
NoneNo IPR availablePANTHERPTHR11757:SF18BNAA08G02180D PROTEINcoord: 46..754
NoneNo IPR availablePANTHERPTHR11757PROTEASE FAMILY S9A OLIGOPEPTIDASEcoord: 46..754
NoneNo IPR availableSUPERFAMILY50993Peptidase/esterase 'gauge' domaincoord: 49..482

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G178620.1Chy10G178620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity