Chy10G178500 (gene) Cucumber (hystrix) v1

Overview
NameChy10G178500
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionFAD synthase
LocationchrH10: 13065898 .. 13070028 (+)
RNA-Seq ExpressionChy10G178500
SyntenyChy10G178500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGCCGCCGGCGTTCGCGTTTCTCACCATCTCCGGGACTTCGACTCTCACTTTGCTTTCGGATTAACCTCCGGCCTTACCTCCTCCGCCTTCCTTCTCTTTCCTCCCATATTGCCTCGCAATCCCACTTCTAATTTTCCTTCAATTTCTCACCGCTCCCAACCACGTCGGGTTTCTTTCTTTTGCTCCATTGTCCCCTCCACTTCCTCCGGTGAAATTCCCGTTCTCTCCGACTGTTTTGGGTAACTTTCTATTCCGTTTCCTTCTATTACTCATGAGCATCCGTGCTGTTTGGTTACAATCTTACCTCTTCGCGTTTTGTTTCCCAGTTCACGAGAGGATGATCGTGAAGTCTCTGTGGCAGGTTTGTGTTCCTTCATTCTTTCTTTTTTCTCCTGAAACTGTTGTCAATTTCATTGAAGAATGCGAATTCGTAATCTCGTCTTAATTATTTTAAGCTTATTTCATTTGAGGGAATGTGAAGTTGAACGACTTTTTGACTATATGATTGTTTGCTTATAGTGCGGGTTCAAGCTGGCTCCGATTTATGTGCAATACCACGAGAATTTGGTTTTGTTAGAAATAGTAAAATACTGCTGGTTGTTCAAGAAGCTCTCAAAAGGGAGTGAAATCAAAATAAAATTCCCAAATAAAGAACAAAGCTATGTCAGTAAGTGTAAAAAAAAATACAAAATTGGAGAACTAAATTCCTATGCTCTCGACAGGATTGAGGATCCTAAGATTTAGGGTATTGATGGGTCCCTAAAGTATATAGTCAGTATATTACAAAGAAAACTTCATTTCGATGGCATTTGTTCCACTTAGTGTTCAAACAGTTGATGGCTTAATGTAGCACGCTGGAATGGAATATAATACACCTATGGAGTCACCAACTTGAGAGCAAGTAGTGAAGACTTAGATATTGTTTTTTTTATTTTTAGAAACAATATTTTAATAAAAAGAAACCAGCTTTCATTGAGGGGGTAAAAATACAGAAGGGGTTACAATATGGACAGCCCAACAAAAAGAGCCAAATCAAGCTAACCTACGTTGGTCACGGTCGGATGGGGATACAGTAGCTTTGATTGATAGTTTTAGGAATGTGTGTCACAGTATTAGATGGATATTCTCCCTCCTAGTTCTAGGTCCTCCAGTTTGGTAAATTATGTGATAAAGTTTTCTTTCCTTCTGTTGGATTAGCTTGCTAATAGAAAGTTGGAAATATAATTCTGTATTTCTCCTATTATTTATAGTTTTGACATTACCATTTCTTTAAAATCATATTTTGTCACTCAGGAGGAATAGTAGCATTAGGAAAATTTGATGCCCTCCACGTTGGTCATCGAGAGCTTGCAATTCAAGCATCAATGGTTGGATCTCCATTTCTATTGTCATTTGTTGGAATTGCTGAAGTACTCGGTTGGGAACCTAGGTAAGGACAATTTGTTTTCTTTGTTTAACTTTATTATTTGTTTCATCATGATCGGTAGGAAAATTACATCTGACTGTTGATGTAGTAGGAATAAGATATTATGTTCAAATTCTTCATCTATTAGAATTGGGACTAGTTTCTTTTATTTCTTAGGATTAGAATTAGTTTCTTTTATTAATTAGGATTAAGATTAGTTTCTTTTATTAATTAGGATTAAGATTAGTTTCTTTTATTAATTAGGATTAGGATTAGTTTGCTATACATAGAGTACTTGTCTTCTTGTATTGACAACTTTTAGAAACATTATTAAAATTCTCTCATTTGGTATACATCAATTGTGCCCTTACAAAATAGAATTTTATGCTGTTTTGATTGTTTTCTAAGGTTCTGACATATTCCACAACTTTATGCAACTTCCCACATTTTCCTGTGGAAGGATTCCTTTTGGTACCAAGAGAATCTGTACGAATGTCTGATCCTCTATTTTTTTTTTCTTCAGTATAACATGAATGATTCAGATTGAGTTTCCAACTTTCCCATGGAATTCTTGCAAATTATAAGGATGTTGTTGTAATCCTATAATGTGAAGTTGGTGGTTTTGGGCGATGTGAAACTCAAAGTATTTTCTCCTAAAAAGGATCCAGTTATGGGACATGAACAATTAATCTCGATGGACTGGGAGTTTAGTTGGGTTCTCTTTTGATAAGAATTTTGATGATCGATTCTCTTCTGGAGTGTAACCATAACTCTTGTTCAGTTGTAGTTAGTGGACAGGTCCAGAAAGATATTCATTAGCTCCTGATAATTTTAAATTCTAATATCTTGTGGTAGCCAATCCCTTTTTCATTAAGTCTTGATGGCTGGCAACAATTAGGTGCAGCCAACAGCAGAAAGATAGCAAGGCACTCCAACATTATGGTTGGCTATTAACAATCTCCAACAAAGAATGGAGGAAAGATTTGGGGTCACTGCTCAAGGCATTCAACATAGTTTAACAGCAATTGAGCAATTAGTTGTTCGTGTTCGAGGATACAAGTTTGCAGAAGGGAACAAAAATTAAAAAAAATAAAGAAAACAGGAGAATATTGCATGAACGGATGGTTAAAAAGAAACAAGAACAAAATCTTTTAAATGAAAAAAACCAAATCCTTTTCTATTGTAAACCATCTAGCTATCATCATGAGAGAATCCGGATGAGCCTTTGGAGACTTCTGCAGACTGCAAGAATCTCAATTCCTGGTAATGTTTGTACTTTGTACAGGTTCCAACTAGCAATTGAGCAACTAGTTACTAACCCCAAAGGGAAATCCTGCTCGGGAAAAGAAATGGATTCCCGTGAGGAATAATTGGAGGAGACACCACATTTGACTGAAAATGTACATTCTATGGAAAATAACTCTGCTAAAGCAGAGGTTTTGGTGAGATTCTAAAAGCGGTGATGATTTGGAGGAAATCGTTAGACGGGGTATCAAAACTATAGATCCTTCTCTTTACTGTTTTAACATTCAAGTTTCTTTTAAATGAAACAAAGTTTCACTGGACCTGGATTAACACATCCCATCAACCTGTATTTGCGTCTGGTCTCTGGTCTCTCTTACCAATACACTCGAATTTCAGGAAATCAAGTATCAGTGTGGTATCCTGTATCCACTGTTTCTTTTTGTAATTGTTTGTAGAACAATGAAAATTCGTCTTAGAAACATCTGATAGTTTGCTAAAATGTTTTATTCTGTTTCTCTACCTGTTTTAGGTAGGCAAATAATGGGAATATGAATACCATCTGTTATGTTCTCAGAGGATTTGTCATGAAGATGTTGAGTTTATTCAGTTGCTGATCTCTAACTCTTCCTTTGTTCTCAACAAGTGCTAAGAATTATACTCTAGGAGTTAATTATTTATCTGTTAGTTAAATTGAACAGGGCTCCCATAGTTGCTCAATGTGATAGGAAGCGAGTTCTTTCCTCATGGGCACCATACTGCCAAAACTCAGCTCCATCAGAGTATCAGATTCAATTTTCAAGCGTCCGTTATCTAACTCCACGAGAATTTGTTGAAAAATTATCAAAGGAGCTTCGTGTTTGTGGAGTTGTGGCAGGTAAGTGGACTTAAAAAGTGTTGTGCAAGTTTTTTATTAATGAAGTACTCTCTTTGGAGTAAAATGTAATCGTTTTGAGTTTCAATTGACGATGATGGCAGGGGAAAGCTATAGGTTTGGATATAAGGCAGCAGGTGATGCAGCAGAGCTGGTGAAACTGTGTGAGGAATATGGGATAAGTGCTTATATTATAAAATCTGTGATGGATAGAAACCAAAAAGTTGTTAATTCTGCCAATTCAAAGGAGAGAGGACAAGTGTCGTCTACTCGGGTTCGCCATGCACTTTCCAAAGGAGATATGAAATATGTCTCTGAGCTTTTAGGCCGCAGGCATCGTCTTATTTTGATGGCCGAAGGCCTAGAAGGATTTAGCAATAGCAATAACAGAGTGTCAGCCCCAAGATCATGTTTATTAAATTTAGCCCCAAAAGAAGGTCTCTATAATAACTGTTTTGTCTGTACAACTGACGAGAACCTAATTCCGTGCCGGGTAGCTATCGACTCCACTCATGTTCACATAGAAATGGATGATATAGGTACAAGCCATCTTGTAGGAACACAAGACCGCGTCAATGTTGAATTTGGCGATGAAGGAGTATGA

mRNA sequence

ATGTTGGCCGCCGGCGTTCGCGTTTCTCACCATCTCCGGGACTTCGACTCTCACTTTGCTTTCGGATTAACCTCCGGCCTTACCTCCTCCGCCTTCCTTCTCTTTCCTCCCATATTGCCTCGCAATCCCACTTCTAATTTTCCTTCAATTTCTCACCGCTCCCAACCACGTCGGGTTTCTTTCTTTTGCTCCATTGTCCCCTCCACTTCCTCCGGTGAAATTCCCGTTCTCTCCGACTGTTTTGGTTCACGAGAGGATGATCGTGAAGTCTCTGTGGCAGGAGGAATAGTAGCATTAGGAAAATTTGATGCCCTCCACGTTGGTCATCGAGAGCTTGCAATTCAAGCATCAATGGTTGGATCTCCATTTCTATTGTCATTTGTTGGAATTGCTGAAGTACTCGGTTGGGAACCTAGGGCTCCCATAGTTGCTCAATGTGATAGGAAGCGAGTTCTTTCCTCATGGGCACCATACTGCCAAAACTCAGCTCCATCAGAGTATCAGATTCAATTTTCAAGCGTCCGTTATCTAACTCCACGAGAATTTGTTGAAAAATTATCAAAGGAGCTTCGTGTTTGTGGAGTTGTGGCAGGGGAAAGCTATAGGTTTGGATATAAGGCAGCAGGTGATGCAGCAGAGCTGGTGAAACTGTGTGAGGAATATGGGATAAGTGCTTATATTATAAAATCTGTGATGGATAGAAACCAAAAAGTTGTTAATTCTGCCAATTCAAAGGAGAGAGGACAAGTGTCGTCTACTCGGGTTCGCCATGCACTTTCCAAAGGAGATATGAAATATGTCTCTGAGCTTTTAGGCCGCAGGCATCGTCTTATTTTGATGGCCGAAGGCCTAGAAGGATTTAGCAATAGCAATAACAGAGTGTCAGCCCCAAGATCATGTTTATTAAATTTAGCCCCAAAAGAAGGTCTCTATAATAACTGTTTTGTCTGTACAACTGACGAGAACCTAATTCCGTGCCGGGTAGCTATCGACTCCACTCATGTTCACATAGAAATGGATGATATAGGTACAAGCCATCTTGTAGGAACACAAGACCGCGTCAATGTTGAATTTGGCGATGAAGGAGTATGA

Coding sequence (CDS)

ATGTTGGCCGCCGGCGTTCGCGTTTCTCACCATCTCCGGGACTTCGACTCTCACTTTGCTTTCGGATTAACCTCCGGCCTTACCTCCTCCGCCTTCCTTCTCTTTCCTCCCATATTGCCTCGCAATCCCACTTCTAATTTTCCTTCAATTTCTCACCGCTCCCAACCACGTCGGGTTTCTTTCTTTTGCTCCATTGTCCCCTCCACTTCCTCCGGTGAAATTCCCGTTCTCTCCGACTGTTTTGGTTCACGAGAGGATGATCGTGAAGTCTCTGTGGCAGGAGGAATAGTAGCATTAGGAAAATTTGATGCCCTCCACGTTGGTCATCGAGAGCTTGCAATTCAAGCATCAATGGTTGGATCTCCATTTCTATTGTCATTTGTTGGAATTGCTGAAGTACTCGGTTGGGAACCTAGGGCTCCCATAGTTGCTCAATGTGATAGGAAGCGAGTTCTTTCCTCATGGGCACCATACTGCCAAAACTCAGCTCCATCAGAGTATCAGATTCAATTTTCAAGCGTCCGTTATCTAACTCCACGAGAATTTGTTGAAAAATTATCAAAGGAGCTTCGTGTTTGTGGAGTTGTGGCAGGGGAAAGCTATAGGTTTGGATATAAGGCAGCAGGTGATGCAGCAGAGCTGGTGAAACTGTGTGAGGAATATGGGATAAGTGCTTATATTATAAAATCTGTGATGGATAGAAACCAAAAAGTTGTTAATTCTGCCAATTCAAAGGAGAGAGGACAAGTGTCGTCTACTCGGGTTCGCCATGCACTTTCCAAAGGAGATATGAAATATGTCTCTGAGCTTTTAGGCCGCAGGCATCGTCTTATTTTGATGGCCGAAGGCCTAGAAGGATTTAGCAATAGCAATAACAGAGTGTCAGCCCCAAGATCATGTTTATTAAATTTAGCCCCAAAAGAAGGTCTCTATAATAACTGTTTTGTCTGTACAACTGACGAGAACCTAATTCCGTGCCGGGTAGCTATCGACTCCACTCATGTTCACATAGAAATGGATGATATAGGTACAAGCCATCTTGTAGGAACACAAGACCGCGTCAATGTTGAATTTGGCGATGAAGGAGTATGA

Protein sequence

MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVSFFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPREFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVNSANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSCLLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGDEGV*
Homology
BLAST of Chy10G178500 vs. ExPASy Swiss-Prot
Match: Q8VZR0 (FAD synthetase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF2 PE=1 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 3.5e-104
Identity = 202/371 (54.45%), Postives = 261/371 (70.35%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPP--ILPRNPTSNFPSISHRSQPRR 60
           ML  G RV  HL D + H + GL  G   +  +      + P    +     S + + R 
Sbjct: 1   MLCGGSRVLQHLSDHNHHNSIGLGLGFCGAKIVQLSSFFLRPSQAMAKSHHFSRKLRQRM 60

Query: 61  VSFFCSIVPSTSSGEIPVLSDCFGSREDDREV------SVAGGIVALGKFDALHVGHREL 120
           +S F S     +SGE+P+L +CF  REDD E+       V+GGIVALGKFDALH+GHREL
Sbjct: 61  ISSFGS--HCRTSGEVPILHNCFSQREDDPELPVEGLSPVSGGIVALGKFDALHIGHREL 120

Query: 121 AIQASMVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFS 180
            IQAS +G+P+LLSFVG+AEVLGWEPRAPIVA+CDR+RVL+SWA YC + AP EY+I+F+
Sbjct: 121 TIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLTSWASYCGDRAPEEYEIEFA 180

Query: 181 SVRYLTPREFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVM 240
           SVR+LTPR+FVEKLSKELRVCGVVAGE+YRFGYKA+GDA+ELV+LCEE GI+A II SVM
Sbjct: 181 SVRHLTPRQFVEKLSKELRVCGVVAGENYRFGYKASGDASELVRLCEECGITACIINSVM 240

Query: 241 DRNQKVV--NSANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNS 300
           D  Q     +S +SK+RGQVSSTRVR AL+ GDM+YVSELLGR HRLIL     +    S
Sbjct: 241 DMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQD--MPS 300

Query: 301 NNRVSAPRSCLLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGT 360
              +S PRS +LNL P  G+Y  C +   DE+ +PC V +D++++H+E +++   +L  +
Sbjct: 301 ERMISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEEVRLCNLDWS 360

BLAST of Chy10G178500 vs. ExPASy Swiss-Prot
Match: Q9FMW8 (FAD synthetase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF1 PE=1 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 7.3e-94
Identity = 190/353 (53.82%), Postives = 246/353 (69.69%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFP--PILPRNPTSNFPSISHRSQPRR 60
           ML  G R S HL D       G    L  S+F+L P   I  +   S+F   SH   PR+
Sbjct: 1   MLCGGSRASVHLWDHRHPPRLG-AKVLRKSSFMLRPCSAISQQRIKSSFR--SHCKTPRK 60

Query: 61  VSFFCSIVPSTSSGEIPVLSDCFGSREDDREVS------VAGGIVALGKFDALHVGHREL 120
                          IP   DCF   +D  E+S      VAGGIVALGKFDALH+GHREL
Sbjct: 61  ---------------IPAPLDCFSQGDDHPELSAEGLSPVAGGIVALGKFDALHIGHREL 120

Query: 121 AIQASMVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFS 180
           AIQA+ +G+P+LLSFVG+AEVLGW+PRAPIVA+CDRKRVLSSWA YC N AP E++I+F+
Sbjct: 121 AIQAARIGTPYLLSFVGLAEVLGWKPRAPIVAKCDRKRVLSSWASYCGNIAPVEFEIEFA 180

Query: 181 SVRYLTPREFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVM 240
           SVR+L P++FVEKLS+ELRVCGVVAGE+YRFGY+A+GDA+ELV+LC+++GISAYII SVM
Sbjct: 181 SVRHLNPQQFVEKLSRELRVCGVVAGENYRFGYRASGDASELVRLCKDFGISAYIINSVM 240

Query: 241 DRNQKVVNS----ANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFS 300
           D+NQ  VN+    + SKERGQVSSTRVRHAL+ GD++YV+ELLGR HR+I      +  +
Sbjct: 241 DKNQVSVNTEEEDSKSKERGQVSSTRVRHALAAGDVRYVTELLGRPHRVISRTR-TQDLT 300

Query: 301 NSNNRVSAPRSCLLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDD 342
           +   R+S   S LLNL P  G+Y  C +   D++ I C+V +D+++++IE ++
Sbjct: 301 SKRGRISLQTSSLLNLPPGNGVYKACSLIVGDKHPISCKVIVDTSNLYIETEE 334

BLAST of Chy10G178500 vs. ExPASy Swiss-Prot
Match: Q10BX9 (FAD synthetase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBF PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 5.4e-89
Identity = 170/289 (58.82%), Postives = 217/289 (75.09%), Query Frame = 0

Query: 76  VLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVGSPFLLSFVGIAEVLG 135
           +L DC G  +D     V  GIVALGKFDALH+GHRELA+ AS  G+PFLLSFVGIAEVLG
Sbjct: 111 LLIDC-GEDQD----CVIDGIVALGKFDALHIGHRELAMYASKAGTPFLLSFVGIAEVLG 170

Query: 136 WEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPREFVEKLSKELRVCGV 195
           WE R PIVAQCDRKRVL+SWAPYC+N  P EYQ++FS VRYLTPR+FVE+LS++L++ GV
Sbjct: 171 WEYRPPIVAQCDRKRVLTSWAPYCKNVVPIEYQVEFSKVRYLTPRQFVERLSRDLKIQGV 230

Query: 196 VAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQK---VVNSANSKERGQVSS 255
           VAGE+YRFGY+A+GDAAELVKLCEE+G+SA+I++SVMD  +    V  S NS ++GQVSS
Sbjct: 231 VAGENYRFGYRASGDAAELVKLCEEFGLSAFIVRSVMDTARSYNGVTTSVNSSDKGQVSS 290

Query: 256 TRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSCLLNLAPKEGLYN 315
           +RVRHAL+ GDM+YVSELLGR+HRL+L  +  E       R+  P+SC+LN+ P +GLY 
Sbjct: 291 SRVRHALAMGDMEYVSELLGRKHRLVLTVK--ENHLQERKRIMLPKSCMLNMPPADGLYE 350

Query: 316 NCFVCTTDENLIPCRVAIDSTHVHIEMDDIGT--SHLVGTQDRVNVEFG 360
           NC +     +L  CRV I+S  + IEM D  +   + +    ++ +EFG
Sbjct: 351 NCDL-LNGGHLGLCRVIINSETIVIEMKDENSLLPNTIQENQQLGIEFG 391

BLAST of Chy10G178500 vs. ExPASy Swiss-Prot
Match: P57250 (Bifunctional riboflavin kinase/FMN adenylyltransferase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=ribF PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-07
Identity = 52/194 (26.80%), Postives = 96/194 (49.48%), Query Frame = 0

Query: 93  AGGIVALGKFDALHVGHRELAIQASMVGSPFLLSFVGIAEVLGWEPR---------APIV 152
           +  +V +G FD +H+GH++L      +G  + LS +    V+ +EP+         AP+ 
Sbjct: 15  SNSVVTIGNFDGIHLGHQKLFSHIYQIGQKYKLSTI----VVLFEPQPLEFLRKNNAPVR 74

Query: 153 AQCDRKRV--LSSWAPYCQNSAPSEYQIQFS-SVRYLTPREF-VEKLSKELRVCGVVAGE 212
               R+++  +SS+      +  S   ++F+ S + L+ ++F +  L  +L +  +V G 
Sbjct: 75  ITKFREKIRRISSY------NFDSILCVKFNKSFQSLSAKDFIINILINKLHLKFIVIGN 134

Query: 213 SYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVNSANSKERGQVSSTRVRHAL 272
            +RFG++  G+   L KL  +Y  +   I+ +   N K            +SST +R AL
Sbjct: 135 DFRFGFQRNGNINLLKKLGYKYQFNVIKIRPLYKNNIK------------ISSTNIRKAL 186

Query: 273 SKGDMKYVSELLGR 274
           S+ ++K  S LLGR
Sbjct: 195 SENNIKLASLLLGR 186

BLAST of Chy10G178500 vs. ExPASy TrEMBL
Match: A0A0A0KPQ8 (FAD synthase OS=Cucumis sativus OX=3659 GN=Csa_5G577970 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 5.8e-203
Identity = 358/363 (98.62%), Postives = 360/363 (99.17%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAAGVRVSHHLRDFDSHFAFGLTSGL SSAFLLFPPI PRNPTSNFPSISHRSQPRRVS
Sbjct: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLASSAFLLFPPIWPRNPTSNFPSISHRSQPRRVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG
Sbjct: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDR+RVLSSWAPYCQNSAPSEY+IQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRQRVLSSWAPYCQNSAPSEYRIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300
           SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC
Sbjct: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300

Query: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360
           LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD
Sbjct: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360

Query: 361 EGV 364
           E V
Sbjct: 361 EVV 363

BLAST of Chy10G178500 vs. ExPASy TrEMBL
Match: A0A5A7STW7 (FAD synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00660 PE=4 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 2.1e-197
Identity = 347/360 (96.39%), Postives = 354/360 (98.33%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAA VRVSHHLR+FDSHFAFGLTSGLTSSA LLFPPI PRNPTSNFPSISHRSQPRRVS
Sbjct: 1   MLAASVRVSHHLREFDSHFAFGLTSGLTSSAILLFPPIWPRNPTSNFPSISHRSQPRRVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG
Sbjct: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300
           SANSKERGQVSSTRVRHALS GDMKYVSELLGRRHRLILMA+GLEGFSN+N+RVSAPRSC
Sbjct: 241 SANSKERGQVSSTRVRHALSIGDMKYVSELLGRRHRLILMAKGLEGFSNTNSRVSAPRSC 300

Query: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360
           LLNL+PKEG YNNCF+CTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQD VN+EFGD
Sbjct: 301 LLNLSPKEGFYNNCFLCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDCVNIEFGD 360

BLAST of Chy10G178500 vs. ExPASy TrEMBL
Match: A0A1S3BD91 (FAD synthase OS=Cucumis melo OX=3656 GN=LOC103488775 PE=4 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 2.1e-197
Identity = 347/360 (96.39%), Postives = 354/360 (98.33%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAA VRVSHHLR+FDSHFAFGLTSGLTSSA LLFPPI PRNPTSNFPSISHRSQPRRVS
Sbjct: 1   MLAASVRVSHHLREFDSHFAFGLTSGLTSSAILLFPPIWPRNPTSNFPSISHRSQPRRVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG
Sbjct: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300
           SANSKERGQVSSTRVRHALS GDMKYVSELLGRRHRLILMA+GLEGFSN+N+RVSAPRSC
Sbjct: 241 SANSKERGQVSSTRVRHALSIGDMKYVSELLGRRHRLILMAKGLEGFSNTNSRVSAPRSC 300

Query: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360
           LLNL+PKEG YNNCF+CTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQD VN+EFGD
Sbjct: 301 LLNLSPKEGFYNNCFLCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDCVNIEFGD 360

BLAST of Chy10G178500 vs. ExPASy TrEMBL
Match: A0A6J1GWB8 (FAD synthase OS=Cucurbita moschata OX=3662 GN=LOC111458123 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.9e-175
Identity = 317/362 (87.57%), Postives = 331/362 (91.44%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAAG RVSHHLR+ DS F FGLTSGLTS   +LFPPI  R PTSNFPSIS RSQPR VS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSI+PSTS GEIPV+SD FGSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS VG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYC+NSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKLSKEL V GVVAGESYRFGYKAAGDAAELVKLCEEYG+ AYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300
           S NS ERGQVSSTRVR+ALS GDMKYVSELLGRRHRLILMA+GLEG SNS  RVSAP+SC
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNS--RVSAPKSC 300

Query: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360
           LLNLAPKEGLYN CFVCT+DE+LIPCRV IDSTHVHIEMDDIGT H VGTQ+ ++VEF  
Sbjct: 301 LLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDH 360

Query: 361 EG 363
           EG
Sbjct: 361 EG 360

BLAST of Chy10G178500 vs. ExPASy TrEMBL
Match: A0A6J1KGG1 (FAD synthase OS=Cucurbita maxima OX=3661 GN=LOC111493664 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 7.4e-174
Identity = 314/364 (86.26%), Postives = 331/364 (90.93%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAAG RVSHHLR+ DS F FGLTSGLTS   +LFPPI  R PTSNFPSIS RSQPR VS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSI+PSTS GEIPV+SD  GSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS VG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSVGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPR PIVAQCDRKRVLSSWAPYC+NSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRPPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKLSKEL V GVVAGESYRFGYKAAGDAAELVKLCEEYG+ AYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGF--SNSNNRVSAPR 300
           S NS ERGQVSSTRVR+ALS GDMKYVSELLGRRHRLILMA+GLEG   SNSN+R+SAP+
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSNSNSRLSAPK 300

Query: 301 SCLLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEF 360
           SCLLNLAPKEGLYN CFVCT+D++LIPCRV IDSTHVHIEMDDIGT H VGTQ+ ++VEF
Sbjct: 301 SCLLNLAPKEGLYNKCFVCTSDDDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360

Query: 361 GDEG 363
             EG
Sbjct: 361 DHEG 364

BLAST of Chy10G178500 vs. NCBI nr
Match: XP_004147108.1 (FAD synthetase 2, chloroplastic [Cucumis sativus] >KGN51558.1 hypothetical protein Csa_008028 [Cucumis sativus])

HSP 1 Score: 714 bits (1844), Expect = 1.62e-259
Identity = 358/363 (98.62%), Postives = 360/363 (99.17%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAAGVRVSHHLRDFDSHFAFGLTSGL SSAFLLFPPI PRNPTSNFPSISHRSQPRRVS
Sbjct: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLASSAFLLFPPIWPRNPTSNFPSISHRSQPRRVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG
Sbjct: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDR+RVLSSWAPYCQNSAPSEY+IQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRQRVLSSWAPYCQNSAPSEYRIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300
           SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC
Sbjct: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300

Query: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360
           LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD
Sbjct: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360

Query: 361 EGV 363
           E V
Sbjct: 361 EVV 363

BLAST of Chy10G178500 vs. NCBI nr
Match: XP_008445891.1 (PREDICTED: FAD synthetase 2, chloroplastic-like [Cucumis melo] >KAA0034078.1 FAD synthetase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 696 bits (1796), Expect = 3.23e-252
Identity = 347/360 (96.39%), Postives = 354/360 (98.33%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAA VRVSHHLR+FDSHFAFGLTSGLTSSA LLFPPI PRNPTSNFPSISHRSQPRRVS
Sbjct: 1   MLAASVRVSHHLREFDSHFAFGLTSGLTSSAILLFPPIWPRNPTSNFPSISHRSQPRRVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG
Sbjct: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300
           SANSKERGQVSSTRVRHALS GDMKYVSELLGRRHRLILMA+GLEGFSN+N+RVSAPRSC
Sbjct: 241 SANSKERGQVSSTRVRHALSIGDMKYVSELLGRRHRLILMAKGLEGFSNTNSRVSAPRSC 300

Query: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360
           LLNL+PKEG YNNCF+CTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQD VN+EFGD
Sbjct: 301 LLNLSPKEGFYNNCFLCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDCVNIEFGD 360

BLAST of Chy10G178500 vs. NCBI nr
Match: XP_038891320.1 (FAD synthetase 2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 662 bits (1708), Expect = 8.65e-239
Identity = 332/363 (91.46%), Postives = 343/363 (94.49%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAAG R SHHLR+ DSHF FG TSGLTSSA LLFP I PR PTSNFPSISHRSQ RRVS
Sbjct: 1   MLAAGARASHHLRECDSHFVFGSTSGLTSSAVLLFPSIWPRYPTSNFPSISHRSQRRRVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSIVPSTSS EIPVLS+CFGSREDDREVSVAGGIVALGKFDALH+GHRELAIQAS VG
Sbjct: 61  FFCSIVPSTSSDEIPVLSNCFGSREDDREVSVAGGIVALGKFDALHIGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSF+GIAEVLGWEPRAPIVAQCDRKRVLSSWAPYC+NSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFIGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKL+KEL VCGVVAGESYRFGYKAAGDAAELVKLCEEYGI AYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLTKELGVCGVVAGESYRFGYKAAGDAAELVKLCEEYGIGAYIIKSVMDRNQKVVN 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300
           SANSKERGQVSSTRVR+ALS GDMKYVSELLGRRHRLILMA+GLEGFSNSN+RVSAPRSC
Sbjct: 241 SANSKERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGFSNSNSRVSAPRSC 300

Query: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360
           LLNLAPKEGLYN CFVCT+DENLIPCRV IDSTHV IEMDDIGT HLVGTQD +NVEFGD
Sbjct: 301 LLNLAPKEGLYNKCFVCTSDENLIPCRVVIDSTHVQIEMDDIGTRHLVGTQDYINVEFGD 360

Query: 361 EGV 363
           EGV
Sbjct: 361 EGV 363

BLAST of Chy10G178500 vs. NCBI nr
Match: KAG6601065.1 (FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 624 bits (1609), Expect = 1.13e-223
Identity = 317/364 (87.09%), Postives = 334/364 (91.76%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAAG RVSHHLR+ DS F FGLTSGLTS   +LFPPI  RNPTSNFPSIS RSQPR VS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRNPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSI+PSTS GEIPV+SD FGSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS VG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYC+NSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKLSKEL V GVVAGESYRFGYKAAGDAAELVKLCEEYG+ AYIIKSVMDRNQKVV+
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVD 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNN--RVSAPR 300
           S NS ERGQVSSTRVR+ALS GDMKYVSELLGRRHRLILMA+GLEG SNSN+  RVSAP+
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSNSNSRVSAPK 300

Query: 301 SCLLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEF 360
           SCLLNLAPKEGLYN CFVCT+D++LIPCRV IDSTHVHIEMDDIGT H VGTQ+ ++VEF
Sbjct: 301 SCLLNLAPKEGLYNKCFVCTSDDDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEF 360

Query: 361 GDEG 362
             EG
Sbjct: 361 DHEG 364

BLAST of Chy10G178500 vs. NCBI nr
Match: XP_022956361.1 (FAD synthetase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 622 bits (1604), Expect = 5.62e-223
Identity = 317/362 (87.57%), Postives = 331/362 (91.44%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPPILPRNPTSNFPSISHRSQPRRVS 60
           MLAAG RVSHHLR+ DS F FGLTSGLTS   +LFPPI  R PTSNFPSIS RSQPR VS
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGLTSFPVVLFPPIWSRKPTSNFPSISRRSQPRGVS 60

Query: 61  FFCSIVPSTSSGEIPVLSDCFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASMVG 120
           FFCSI+PSTS GEIPV+SD FGSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS VG
Sbjct: 61  FFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQASTVG 120

Query: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFSSVRYLTPR 180
           SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYC+NSAPSEYQIQFSSVRYLTPR
Sbjct: 121 SPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYLTPR 180

Query: 181 EFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVMDRNQKVVN 240
           EFVEKLSKEL V GVVAGESYRFGYKAAGDAAELVKLCEEYG+ AYIIKSVMDRNQKVVN
Sbjct: 181 EFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQKVVN 240

Query: 241 SANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNSNNRVSAPRSC 300
           S NS ERGQVSSTRVR+ALS GDMKYVSELLGRRHRLILMA+GLEG SNS  RVSAP+SC
Sbjct: 241 STNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNS--RVSAPKSC 300

Query: 301 LLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGTQDRVNVEFGD 360
           LLNLAPKEGLYN CFVCT+DE+LIPCRV IDSTHVHIEMDDIGT H VGTQ+ ++VEF  
Sbjct: 301 LLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGTRHPVGTQNYLSVEFDH 360

Query: 361 EG 362
           EG
Sbjct: 361 EG 360

BLAST of Chy10G178500 vs. TAIR 10
Match: AT5G08340.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 379.8 bits (974), Expect = 2.5e-105
Identity = 202/371 (54.45%), Postives = 261/371 (70.35%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPP--ILPRNPTSNFPSISHRSQPRR 60
           ML  G RV  HL D + H + GL  G   +  +      + P    +     S + + R 
Sbjct: 1   MLCGGSRVLQHLSDHNHHNSIGLGLGFCGAKIVQLSSFFLRPSQAMAKSHHFSRKLRQRM 60

Query: 61  VSFFCSIVPSTSSGEIPVLSDCFGSREDDREV------SVAGGIVALGKFDALHVGHREL 120
           +S F S     +SGE+P+L +CF  REDD E+       V+GGIVALGKFDALH+GHREL
Sbjct: 61  ISSFGS--HCRTSGEVPILHNCFSQREDDPELPVEGLSPVSGGIVALGKFDALHIGHREL 120

Query: 121 AIQASMVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFS 180
            IQAS +G+P+LLSFVG+AEVLGWEPRAPIVA+CDR+RVL+SWA YC + AP EY+I+F+
Sbjct: 121 TIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLTSWASYCGDRAPEEYEIEFA 180

Query: 181 SVRYLTPREFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVM 240
           SVR+LTPR+FVEKLSKELRVCGVVAGE+YRFGYKA+GDA+ELV+LCEE GI+A II SVM
Sbjct: 181 SVRHLTPRQFVEKLSKELRVCGVVAGENYRFGYKASGDASELVRLCEECGITACIINSVM 240

Query: 241 DRNQKVV--NSANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNS 300
           D  Q     +S +SK+RGQVSSTRVR AL+ GDM+YVSELLGR HRLIL     +    S
Sbjct: 241 DMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQD--MPS 300

Query: 301 NNRVSAPRSCLLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDDIGTSHLVGT 360
              +S PRS +LNL P  G+Y  C +   DE+ +PC V +D++++H+E +++   +L  +
Sbjct: 301 ERMISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEEVRLCNLDWS 360

BLAST of Chy10G178500 vs. TAIR 10
Match: AT5G08340.2 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 5.2e-103
Identity = 195/351 (55.56%), Postives = 249/351 (70.94%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFPP--ILPRNPTSNFPSISHRSQPRR 60
           ML  G RV  HL D + H + GL  G   +  +      + P    +     S + + R 
Sbjct: 1   MLCGGSRVLQHLSDHNHHNSIGLGLGFCGAKIVQLSSFFLRPSQAMAKSHHFSRKLRQRM 60

Query: 61  VSFFCSIVPSTSSGEIPVLSDCFGSREDDREV------SVAGGIVALGKFDALHVGHREL 120
           +S F S     +SGE+P+L +CF  REDD E+       V+GGIVALGKFDALH+GHREL
Sbjct: 61  ISSFGS--HCRTSGEVPILHNCFSQREDDPELPVEGLSPVSGGIVALGKFDALHIGHREL 120

Query: 121 AIQASMVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFS 180
            IQAS +G+P+LLSFVG+AEVLGWEPRAPIVA+CDR+RVL+SWA YC + AP EY+I+F+
Sbjct: 121 TIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLTSWASYCGDRAPEEYEIEFA 180

Query: 181 SVRYLTPREFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVM 240
           SVR+LTPR+FVEKLSKELRVCGVVAGE+YRFGYKA+GDA+ELV+LCEE GI+A II SVM
Sbjct: 181 SVRHLTPRQFVEKLSKELRVCGVVAGENYRFGYKASGDASELVRLCEECGITACIINSVM 240

Query: 241 DRNQKVV--NSANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFSNS 300
           D  Q     +S +SK+RGQVSSTRVR AL+ GDM+YVSELLGR HRLIL     +    S
Sbjct: 241 DMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQD--MPS 300

Query: 301 NNRVSAPRSCLLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDD 342
              +S PRS +LNL P  G+Y  C +   DE+ +PC V +D++++H+E ++
Sbjct: 301 ERMISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEE 347

BLAST of Chy10G178500 vs. TAIR 10
Match: AT5G23330.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 345.5 bits (885), Expect = 5.2e-95
Identity = 190/353 (53.82%), Postives = 246/353 (69.69%), Query Frame = 0

Query: 1   MLAAGVRVSHHLRDFDSHFAFGLTSGLTSSAFLLFP--PILPRNPTSNFPSISHRSQPRR 60
           ML  G R S HL D       G    L  S+F+L P   I  +   S+F   SH   PR+
Sbjct: 1   MLCGGSRASVHLWDHRHPPRLG-AKVLRKSSFMLRPCSAISQQRIKSSFR--SHCKTPRK 60

Query: 61  VSFFCSIVPSTSSGEIPVLSDCFGSREDDREVS------VAGGIVALGKFDALHVGHREL 120
                          IP   DCF   +D  E+S      VAGGIVALGKFDALH+GHREL
Sbjct: 61  ---------------IPAPLDCFSQGDDHPELSAEGLSPVAGGIVALGKFDALHIGHREL 120

Query: 121 AIQASMVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCQNSAPSEYQIQFS 180
           AIQA+ +G+P+LLSFVG+AEVLGW+PRAPIVA+CDRKRVLSSWA YC N AP E++I+F+
Sbjct: 121 AIQAARIGTPYLLSFVGLAEVLGWKPRAPIVAKCDRKRVLSSWASYCGNIAPVEFEIEFA 180

Query: 181 SVRYLTPREFVEKLSKELRVCGVVAGESYRFGYKAAGDAAELVKLCEEYGISAYIIKSVM 240
           SVR+L P++FVEKLS+ELRVCGVVAGE+YRFGY+A+GDA+ELV+LC+++GISAYII SVM
Sbjct: 181 SVRHLNPQQFVEKLSRELRVCGVVAGENYRFGYRASGDASELVRLCKDFGISAYIINSVM 240

Query: 241 DRNQKVVNS----ANSKERGQVSSTRVRHALSKGDMKYVSELLGRRHRLILMAEGLEGFS 300
           D+NQ  VN+    + SKERGQVSSTRVRHAL+ GD++YV+ELLGR HR+I      +  +
Sbjct: 241 DKNQVSVNTEEEDSKSKERGQVSSTRVRHALAAGDVRYVTELLGRPHRVISRTR-TQDLT 300

Query: 301 NSNNRVSAPRSCLLNLAPKEGLYNNCFVCTTDENLIPCRVAIDSTHVHIEMDD 342
           +   R+S   S LLNL P  G+Y  C +   D++ I C+V +D+++++IE ++
Sbjct: 301 SKRGRISLQTSSLLNLPPGNGVYKACSLIVGDKHPISCKVIVDTSNLYIETEE 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZR03.5e-10454.45FAD synthetase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF2 PE=1 SV... [more]
Q9FMW87.3e-9453.82FAD synthetase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF1 PE=1 SV... [more]
Q10BX95.4e-8958.82FAD synthetase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBF P... [more]
P572501.4e-0726.80Bifunctional riboflavin kinase/FMN adenylyltransferase OS=Buchnera aphidicola su... [more]
Match NameE-valueIdentityDescription
A0A0A0KPQ85.8e-20398.62FAD synthase OS=Cucumis sativus OX=3659 GN=Csa_5G577970 PE=4 SV=1[more]
A0A5A7STW72.1e-19796.39FAD synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00660 PE... [more]
A0A1S3BD912.1e-19796.39FAD synthase OS=Cucumis melo OX=3656 GN=LOC103488775 PE=4 SV=1[more]
A0A6J1GWB83.9e-17587.57FAD synthase OS=Cucurbita moschata OX=3662 GN=LOC111458123 PE=4 SV=1[more]
A0A6J1KGG17.4e-17486.26FAD synthase OS=Cucurbita maxima OX=3661 GN=LOC111493664 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147108.11.62e-25998.62FAD synthetase 2, chloroplastic [Cucumis sativus] >KGN51558.1 hypothetical prote... [more]
XP_008445891.13.23e-25296.39PREDICTED: FAD synthetase 2, chloroplastic-like [Cucumis melo] >KAA0034078.1 FAD... [more]
XP_038891320.18.65e-23991.46FAD synthetase 2, chloroplastic-like [Benincasa hispida][more]
KAG6601065.11.13e-22387.09FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022956361.15.62e-22387.57FAD synthetase 2, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G08340.12.5e-10554.45Nucleotidylyl transferase superfamily protein [more]
AT5G08340.25.2e-10355.56Nucleotidylyl transferase superfamily protein [more]
AT5G23330.15.2e-9553.82Nucleotidylyl transferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 84..274
e-value: 4.0E-35
score: 123.0
IPR015864FAD synthetasePFAMPF06574FAD_syncoord: 174..240
e-value: 8.8E-10
score: 38.7
NoneNo IPR availablePANTHERPTHR12714:SF20FAD SYNTHETASE 1, CHLOROPLASTIC-RELATEDcoord: 7..360
NoneNo IPR availablePANTHERPTHR12714PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASEcoord: 7..360
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 96..273

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G178500.1Chy10G178500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006747 FAD biosynthetic process
biological_process GO:0009231 riboflavin biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0003919 FMN adenylyltransferase activity