Chy10G174820 (gene) Cucumber (hystrix) v1

Overview
NameChy10G174820
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptioncytochrome P450 714A1-like
LocationchrH10: 7196698 .. 7201609 (-)
RNA-Seq ExpressionChy10G174820
SyntenyChy10G174820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGGTATGTAATGAGTGGTTTTATGTTGGTTTTTCCGACCGCGATTCTGGCGGCGGTGGTGGCGATATTGCTCCATTTTTACTCCTACGGTGCGTGGAGGAGGACGGCGGAGGTGAGAAGGAAGTTGGGAGCACAAGGCGTGACTGGACCGCCACCGTCCATTTTGTATGGTAACTTGCCGGAGATGCAAAAGATCCAGTTGCAAACGGCTGCTATGGCTTCTCCATTGCATCGTGCTTCCGCGATTGTCGCTCATGATTATACATCAACTCTCTTTCCCTATTTCGTCGAGTGGAGAAAGCAATATGGTATTTTTGTTAATTTCTATCCTTTTGTATTTTTAGGTTAAATTATAACGAGTCAGGTTGATAGAAGTTCTATCTAAGTAAATAGTTTGACAATTTTTTTTTAAATTTTTTTTAAATTTTCTTATTTTTTTCACTCTCAAACAATATTAAATTATTTTCATACAAATTTTAATTTTATGAAATGTTACATTTAATTGAGAGTATGTGTTGTATGTTGAGTGAGTGGACCATTGAAAAATAATGAATTAATTTTATATGCAATCCAAACTTCTAGGAATAATATAATTTTAAAAAGAAAATCTCCTCCATTGTAATGATATACACACACATATATATTTTATTGTTTTGAGACAATATTATAATTTTATACGAGTAGTGGAGATTTTAATATTCCGACTAAAAATAAATATTATTGTTGCCTCAATCATTCCACCGTCTAAAAGATTGAACAAAATTTATTACACGTTTTGTAAATTCAATATTAGTATTTTTCTTTTTTTTTTGAAAAAATAAAATTTAAATAGATAAAAGTAGTTTTTATGAATAGTTTCAAAAGTCAATTCTCATAACCTCGACTTCAACCCAACTTCGTGTCCATTAAATAGTGAATAATAATAAATACACATTTTATTTGAACGCGTTTACTATTTTTAATCACATCTCCATCTTATTTTTTGGATCAAAGGTTTAATTATATGAGTTTAATAACTTAAATATTTCATCTAAAAAATAAAAATCCTTTGATAACATTAGATGAAGTTCGTTTCCTATATCAAATTTCTCCACCTTTTAATGGATATCCTCAACAAACAAAAATCATTAAAAAATATATATTTATTTAATTAAATTTATTTTATAACATTAATTTTTTTTTTTGTTTTTTGTTTTTCATTGCCACAATATATCTGCTCTATTCAAAATAACCAGAATGCATACAAAACTTCAAAAAACTCTCTAACACAATCCACAACAGTTGGCTACATTAGAATTGTCCTTTTCAGTAATTTGTCCTAAGTGCCTCTTTTGTTATAATACAAATAGATTAGGTTAAAATTTCATATTTTGAACATTATTTAGTTTCTATACTTTTTAAAATTACATTATTGTAACCTTTAAACTTTTCAATTTTTTTAAAATTCTCACATATAATGGATTGAAAATTTTTAGAATTCTTTTGTAATAGAGAAGAATGACTTTAAAAAAAATTGAATTGGTTAAGAACTTATTAGACATACAGTTTATTAACTCTATTAATTACACAACTTAAAGTTTAATGTTTTTAATATTAGACAATTTTAAAAGTTGGGTGATAAATAAACTAAACAGTACAGACCGAAACACGAGACGATGAAACACAAGAATGGGGCATGGACGAGTAAATAGGAGAGGCCCTATGAATTGAATGTAGAGAAACTTTGAGATGTTGTTTGTTTGGTTTGGCAGGGCCGTTATACACGTACTCGACAGGGACAAGACAGCATCTTTACGCCAACAAGGTTGAACTTGTGAAGGACTTGAGCCTCTCCAACAGTCTCAACGTGGGGAAGCCTTTCTATGTCACCAAAAAGCTTGCTCCCATTCTCGGCCAAAGCGTCGTCCGCTCTAACGGCGCTGTTTGGGCAGTGCAACGGAAGGTCATTGCCCCCGAGTTCTTCATGGACCGTGTCAGGGCCATGGCGTTCCACATGGTCGATGCTGCATCGTGTCTTATCAACAAATGGGAAAGCAGGGTTGGAGATGGAACCATCGAGATTGATGTCGATGAAGATCTAAGAGGGTTCTCGGCTGACGTCATATCTAGAGCTTGCTTTGGGAGCTCATATGAGAAGGGGAAGGAGATTTTTTCCAAGCTTAGGGATCTCCAGAAGTTGATTTGTGAGGATAGCTTTCTGTTTGGTTACAGTTCATGGAGGTTATTTTCTCTCCTTTTTCTTTCTTCACTACTAATTTTATTAATACTAAAAAAATACAAATAAAAATAAAATATAGAAATAATTATTATAGTTCATGCTTAGTTTCTCTATTTTATTCAATCCATTAAATTTGAAGGGATTTGAATAACAACTTGTTCAGTTTTAAATTCGTTTTTTAGAATATAAACTATAATTATCAAGATTTAAATTATTGATTCATAGGAATGTTTATTAAAAATGAGTAATATTTGGATACATCAAGTAACATACATCTTTTTACTTCCCTTCAACAAAAAAAATTACTTTAAAAGAAAATTGAAAAAAAGATTGTCAAACAATTGAATCTGAGTCAGCTGAGTCAGCTGCCTACACACCAGCATAAATGCCTACGTACATTTTGATGTGTCACATTTGTATTGGCTCATTAGATCACTCATTTAATGCAATAGAATACTATTGAAACATGTTCTAAATATCTTCCACCATAATTAATTCTTTTATTAAATATTTTCTAATACACTAAAATAAACTTTGAATAAAAACTTTTTGGATGCATATTTAAGTGTACATATAGTTCTTTTCTTCCAGCTGGCTATATATTTTATAGATTATCTTTGATCACTTAAAATTTTTTCTAATCTATCCGAAAATTATTCAAAAATAGTTTTAAATTAATTCAACATTTTCATTGTTTTTATTTACTAAGATAAGATTGTTTTAAAAAATATTTGATCAAAGAAATTTGACAACTTTGGAAGAGTTGGGTTAGGTAGATAAAAACAAACTAACCCTTTTTTTGATTCACCAAAAATTAAAAGGATTTATTATATTGTTATTTGTATTAACTCACATTTCTCACTAAAATATTCATATTTTTCACATTGTTATTCTTGTCAACTTCTTACCCGTTGATCAACTTAACTCTTTATTTTTGTTACTAATCAAACGGCTCCTAATAAAGTAAATGTACCAAATGTGGCAAGTATTATTATTATTTTTTGAAAAATAGTCGTTGAAAATTGATTTTATTAATTAGAATCACTTTATATATATTTGAACAATAAAAATAAGCCAATAAATTAAAAAAAGAAAAAGAAAAAGAGAAGAAGAATCTCTTTAGACTGATAATAATTGAGGAGCTGAATTGTGTTTAAGTTGATAATAGCAATAAGATTAATAAAGGTTTTAATTTGATGAACAGTGATAAATTTTTGCAACCACGCAAACACAAAAGAATAAATAGGTTGGAGAAGGAGATTGAGTCATTAATATGGGAGACGGTTCAACAACGACAAAAAGAATGCTCTAAAACCTCCTCGTCGGACAAGGATTTGTTGCAGCTAATAATGGAAGCCACCATCGATGACCCAAATATAGGAGCGAAGGACTCTTCCAAGAACTTCATTGTTGACAACTGCAAGAGCATCTACTTCGCCGGCCATGAGTCCACCGCCGTGGCCGCCACCTGGTCCTTGATGCTCCTCGCCCTCCACCCCGAATGGCAAGACCGTATCCGCTCTGAGTTTGCTCAGGCTTGTCCCGACGGCCACCTCGACCCGACTGCGACTTCACAACTCAAATCGGTATTCTCTCTCTCTCAAAGATATAGTTTGTTTTAGATTGTACAATAATATGTGATTTTGCAGGAGTAGAAGTGGTTTAATTAGGTTTATTTCTCTTTACAAATTATAGCTAACATTTTAAAAAATTATAAATATAGCCAAATTTATCGATTAAATGTCTATGGTGGATAAACTTTAACAGATTTTGATATATTTATCTTTTTTTAAAAAAATGTTATATATGCTAATATTTTAAATCTATACATGCTATATATATTAATATTTTGAATCTAATTGCTTTATGTAATTATCTTTTTTTTTTTTTTTTTGTTAAGTTGTTTAGTTTTGGAGTCCAATAGTATATAAAAAGTTAAATGAGTTAAGAGCCTGATGTTTTATAGCTCTGAAGTTTTCGAACTTTCGGAACTAATCAAGAAGTGGATCACTTACTTCTTTGACACTAACACCTACTCCTTAGATAATGGGTCTTTAAAACTTTTTGCGGAGAAAAATAAGATAATAAAAAATCATGAATTTTAAGATGATGATGAGGAAAAAAGAAGGGTATCGGTGGAATCTAAGTTTTTAGGAGAATGGACTAAATTGTTGCAAATTTAAAATACTAACGGATTGCAAATGAAAAGTAAATGGTGGACACATATACTCTCCTAATAGCATGAAACAACTGGTGGATGTAGGTGAGTATGGTGATTCACGAAACGCTACGATTGTACCCACCAGCTGCATTTGTGGCGAGGGAGACATTTGCTGAGACACAACTTGGGAATGTGGTGGTTCCAAAGGGTGTTTGCTTATGGACTTTAATTCCGACGCTGCACAGAGAAGTCGAAATATGGGGAGAAGATGCAAATGAGTTCAAGCCAGAGCGATTCGCAAATGGCGTAGCCAAAGCATGCAAGTTCCCTCAGGCCTACGTGCCATTTGGGGCAGGCCCTAGACTATGTTTGGGGAAGAACTTCGCATTAGTTGAATTGAAGATCATAATTTCTCTTATAGTTTCCAAGTTTAGATTCTCGTTGTCTCCTGAGTATCACCATTGCCCTTCATATAGAATGATTGTGGAGCCTGCAAATGGTGTGAAGATTGTTTTCCAAAGGCTATGA

mRNA sequence

ATGGAAGGGTATGTAATGAGTGGTTTTATGTTGGTTTTTCCGACCGCGATTCTGGCGGCGGTGGTGGCGATATTGCTCCATTTTTACTCCTACGGTGCGTGGAGGAGGACGGCGGAGGTGAGAAGGAAGTTGGGAGCACAAGGCGTGACTGGACCGCCACCGTCCATTTTGTATGGTAACTTGCCGGAGATGCAAAAGATCCAGTTGCAAACGGCTGCTATGGCTTCTCCATTGCATCGTGCTTCCGCGATTGTCGCTCATGATTATACATCAACTCTCTTTCCCTATTTCGTCGAGTGGAGAAAGCAATATGGGCCGTTATACACGTACTCGACAGGGACAAGACAGCATCTTTACGCCAACAAGGTTGAACTTGTGAAGGACTTGAGCCTCTCCAACAGTCTCAACGTGGGGAAGCCTTTCTATGTCACCAAAAAGCTTGCTCCCATTCTCGGCCAAAGCGTCGTCCGCTCTAACGGCGCTGTTTGGGCAGTGCAACGGAAGGTCATTGCCCCCGAGTTCTTCATGGACCGTGTCAGGGCCATGGCGTTCCACATGGTCGATGCTGCATCGTGTCTTATCAACAAATGGGAAAGCAGGGTTGGAGATGGAACCATCGAGATTGATGTCGATGAAGATCTAAGAGGGTTCTCGGCTGACGTCATATCTAGAGCTTGCTTTGGGAGCTCATATGAGAAGGGGAAGGAGATTTTTTCCAAGCTTAGGGATCTCCAGAAGTTGATTTGTGAGGATAGCTTTCTGTTTGGTTACAGTTCATGGAGTGATAAATTTTTGCAACCACGCAAACACAAAAGAATAAATAGGTTGGAGAAGGAGATTGAGTCATTAATATGGGAGACGGTTCAACAACGACAAAAAGAATGCTCTAAAACCTCCTCGTCGGACAAGGATTTGTTGCAGCTAATAATGGAAGCCACCATCGATGACCCAAATATAGGAGCGAAGGACTCTTCCAAGAACTTCATTGTTGACAACTGCAAGAGCATCTACTTCGCCGGCCATGAGTCCACCGCCGTGGCCGCCACCTGGTCCTTGATGCTCCTCGCCCTCCACCCCGAATGGCAAGACCGTATCCGCTCTGAGTTTGCTCAGGCTTGTCCCGACGGCCACCTCGACCCGACTGCGACTTCACAACTCAAATCGGTGAGTATGGTGATTCACGAAACGCTACGATTGTACCCACCAGCTGCATTTGTGGCGAGGGAGACATTTGCTGAGACACAACTTGGGAATGTGGTGGTTCCAAAGGGTGTTTGCTTATGGACTTTAATTCCGACGCTGCACAGAGAAGTCGAAATATGGGGAGAAGATGCAAATGAGTTCAAGCCAGAGCGATTCGCAAATGGCGTAGCCAAAGCATGCAAGTTCCCTCAGGCCTACGTGCCATTTGGGGCAGGCCCTAGACTATGTTTGGGGAAGAACTTCGCATTAGTTGAATTGAAGATCATAATTTCTCTTATAGTTTCCAAGTTTAGATTCTCGTTGTCTCCTGAGTATCACCATTGCCCTTCATATAGAATGATTGTGGAGCCTGCAAATGGTGTGAAGATTGTTTTCCAAAGGCTATGA

Coding sequence (CDS)

ATGGAAGGGTATGTAATGAGTGGTTTTATGTTGGTTTTTCCGACCGCGATTCTGGCGGCGGTGGTGGCGATATTGCTCCATTTTTACTCCTACGGTGCGTGGAGGAGGACGGCGGAGGTGAGAAGGAAGTTGGGAGCACAAGGCGTGACTGGACCGCCACCGTCCATTTTGTATGGTAACTTGCCGGAGATGCAAAAGATCCAGTTGCAAACGGCTGCTATGGCTTCTCCATTGCATCGTGCTTCCGCGATTGTCGCTCATGATTATACATCAACTCTCTTTCCCTATTTCGTCGAGTGGAGAAAGCAATATGGGCCGTTATACACGTACTCGACAGGGACAAGACAGCATCTTTACGCCAACAAGGTTGAACTTGTGAAGGACTTGAGCCTCTCCAACAGTCTCAACGTGGGGAAGCCTTTCTATGTCACCAAAAAGCTTGCTCCCATTCTCGGCCAAAGCGTCGTCCGCTCTAACGGCGCTGTTTGGGCAGTGCAACGGAAGGTCATTGCCCCCGAGTTCTTCATGGACCGTGTCAGGGCCATGGCGTTCCACATGGTCGATGCTGCATCGTGTCTTATCAACAAATGGGAAAGCAGGGTTGGAGATGGAACCATCGAGATTGATGTCGATGAAGATCTAAGAGGGTTCTCGGCTGACGTCATATCTAGAGCTTGCTTTGGGAGCTCATATGAGAAGGGGAAGGAGATTTTTTCCAAGCTTAGGGATCTCCAGAAGTTGATTTGTGAGGATAGCTTTCTGTTTGGTTACAGTTCATGGAGTGATAAATTTTTGCAACCACGCAAACACAAAAGAATAAATAGGTTGGAGAAGGAGATTGAGTCATTAATATGGGAGACGGTTCAACAACGACAAAAAGAATGCTCTAAAACCTCCTCGTCGGACAAGGATTTGTTGCAGCTAATAATGGAAGCCACCATCGATGACCCAAATATAGGAGCGAAGGACTCTTCCAAGAACTTCATTGTTGACAACTGCAAGAGCATCTACTTCGCCGGCCATGAGTCCACCGCCGTGGCCGCCACCTGGTCCTTGATGCTCCTCGCCCTCCACCCCGAATGGCAAGACCGTATCCGCTCTGAGTTTGCTCAGGCTTGTCCCGACGGCCACCTCGACCCGACTGCGACTTCACAACTCAAATCGGTGAGTATGGTGATTCACGAAACGCTACGATTGTACCCACCAGCTGCATTTGTGGCGAGGGAGACATTTGCTGAGACACAACTTGGGAATGTGGTGGTTCCAAAGGGTGTTTGCTTATGGACTTTAATTCCGACGCTGCACAGAGAAGTCGAAATATGGGGAGAAGATGCAAATGAGTTCAAGCCAGAGCGATTCGCAAATGGCGTAGCCAAAGCATGCAAGTTCCCTCAGGCCTACGTGCCATTTGGGGCAGGCCCTAGACTATGTTTGGGGAAGAACTTCGCATTAGTTGAATTGAAGATCATAATTTCTCTTATAGTTTCCAAGTTTAGATTCTCGTTGTCTCCTGAGTATCACCATTGCCCTTCATATAGAATGATTGTGGAGCCTGCAAATGGTGTGAAGATTGTTTTCCAAAGGCTATGA

Protein sequence

MEGYVMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGNLPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL*
Homology
BLAST of Chy10G174820 vs. ExPASy Swiss-Prot
Match: Q93Z79 (Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.3e-165
Identity = 294/499 (58.92%), Postives = 371/499 (74.35%), Query Frame = 0

Query: 34  WRRTAEVRRKLGAQGVTGPPPSILYGNLPEMQKIQLQTAAMASPLHRASAIVAHDYTSTL 93
           WR    +RRKL  QGV GPPPS+  GN+PEMQKIQ Q  +  S  +    I+AHDYTS+L
Sbjct: 36  WR----MRRKLTMQGVKGPPPSLFRGNVPEMQKIQSQIMS-NSKHYSGDNIIAHDYTSSL 95

Query: 94  FPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQ 153
           FPY   WRKQYG +YTYSTG +QHLY N  ELVK+L+ +N+LN+GK  YVTK+L  ILG+
Sbjct: 96  FPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQANTLNLGKVSYVTKRLKSILGR 155

Query: 154 SVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCLINKWE---SRVGDGTIEIDV 213
            V+ SNG  WA QR++IAPEFF+D+V+ M   +V++A  +++KWE    R G+   +I V
Sbjct: 156 GVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPMLSKWEEMMKREGEMVCDIIV 215

Query: 214 DEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICEDSFLFGYSSWSDKFLQPRKH 273
           DEDLR  SADVISRACFGSS+ KGKEIFSKLR LQK I  ++ LF  + ++D     +KH
Sbjct: 216 DEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITHNNILFSLNGFTDVVFGTKKH 275

Query: 274 --KRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQLIMEATID--DPNIGAKDSS- 333
              +I+ LE+ IESLIWETV++R++EC       KDL+QLI+E      D N+  K  S 
Sbjct: 276 GNGKIDELERHIESLIWETVKEREREC--VGDHKKDLMQLILEGARSSCDGNLEDKTQSY 335

Query: 334 KNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIRSEFAQACPDGHLDPTATSQ 393
           K+F+VDNCKSIYFAGHE++AVA +W LMLLAL+P WQ RIR E    C +G  D  + S 
Sbjct: 336 KSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRIRDEVFLHCKNGIPDADSISN 395

Query: 394 LKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVCLWTLIPTLHREVEIWGEDA 453
           LK+V+MVI ETLRLYPPAAFV+RE   +T+LGN+VVPKGVC+WTLIPTLHR+ EIWG DA
Sbjct: 396 LKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGVCIWTLIPTLHRDPEIWGADA 455

Query: 454 NEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVELKIIISLIVSKFRFSLSPE 513
           NEF PERF+ GV+KACK PQ++VPFG G RLCLGKNF ++ELK+++SLIVS+F F+LSP 
Sbjct: 456 NEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMMELKVLVSLIVSRFSFTLSPT 515

Query: 514 YHHCPSYRMIVEPANGVKI 525
           Y H P +RM+VEP +GV I
Sbjct: 516 YQHSPVFRMLVEPQHGVVI 527

BLAST of Chy10G174820 vs. ExPASy Swiss-Prot
Match: Q6NKZ8 (Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 3.5e-153
Identity = 267/518 (51.54%), Postives = 366/518 (70.66%), Query Frame = 0

Query: 17  ILAAVVAILLHFYSYGA---WRRTAEVRRKLGAQGVTGPPPSILYGNLPEMQKIQLQTAA 76
           ++  + ++  H Y       WR    +RR L  QGV GPPPSI  GN+ EMQ+IQ +   
Sbjct: 16  VIVGIFSVGYHVYGRAVVEQWR----MRRSLKLQGVKGPPPSIFNGNVSEMQRIQSEAKH 75

Query: 77  MASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSN 136
            +        I++HDY+S+LFP+F  WRKQYG +YTYSTG +QHLY N  E+VK+LS +N
Sbjct: 76  CS-----GDNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTN 135

Query: 137 SLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCL 196
           +LN+G+  ++TK+L PILG  ++ SNG  WA QR++IA EF  D+++ M   MV++A  +
Sbjct: 136 TLNLGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPM 195

Query: 197 INKWESRV---GDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICE 256
           +NKWE  V   G+   +I VDEDL+  SADVI++ACFGSS+ KGK IFS +RDL   I +
Sbjct: 196 LNKWEEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITK 255

Query: 257 DSFLFGYSSWSDKFLQPRKH--KRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQL 316
            S LF ++ ++D     +KH    I+ LE E+ES IWETV++R+ EC  T    KDL+QL
Sbjct: 256 RSVLFRFNGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHK--KDLMQL 315

Query: 317 IMEATID--DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIR 376
           I+E  +   D N+  K + + F+VDNCKSIYFAGH+STAV+ +W LMLLAL+P WQ +IR
Sbjct: 316 ILEGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIR 375

Query: 377 SEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVC 436
            E   +C +G  D  +   LK+V+MVI ET+RLYPPA  V RE   + +LG++VVPKGVC
Sbjct: 376 DEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVC 435

Query: 437 LWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVE 496
           +WTLIP LHR+ EIWG DAN+FKPERF+ G++KACK+PQ+Y+PFG GPR C+GKNF ++E
Sbjct: 436 IWTLIPALHRDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMME 495

Query: 497 LKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKI 525
           +K+++SLIVSKF F+LSP Y H PS++++VEP +GV I
Sbjct: 496 VKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVI 522

BLAST of Chy10G174820 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.7e-131
Identity = 244/532 (45.86%), Postives = 341/532 (64.10%), Query Frame = 0

Query: 1   MEGYVMSGFMLVFPTAILAAVVAILLHFYSY-GAWRRTAEVRRKLGAQGVTGPPPSILYG 60
           ME +    ++ + P  IL     ILL  Y Y   W R   +R+KL +QGV GP PS L+G
Sbjct: 1   MELFSSQQWLALLPPIIL----CILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFG 60

Query: 61  NLPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLY 120
           N+PEM++IQ    +       ++ + + +Y +TLFPYF+ W + YG +Y YSTG+ Q L 
Sbjct: 61  NIPEMRRIQQLAKSAHEQEAGSTDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLN 120

Query: 121 ANKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRV 180
                +VK+L+   SL++GKP Y+ K+   +LG  ++ SNG +W  QRKVIAPE FM+RV
Sbjct: 121 VTDPNMVKELANCKSLDLGKPCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERV 180

Query: 181 RAMAFHMVDAASCLINKWESRVGD--GTIEIDVDEDLRGFSADVISRACFGSSYEKGKEI 240
           + M   M++AA  ++N W++ V D  G+ EI VDE LR FSADVISRACFGSS+ +GKEI
Sbjct: 181 KGMVNLMMEAAMSMLNSWKNEVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEI 240

Query: 241 FSKLRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSK 300
           F K+R LQK + + S L G      ++L  R ++ I  L+  I +LI      ++ E   
Sbjct: 241 FIKIRQLQKAMAKQSMLIGVP--GSRYLPTRSNRGIWNLDSSIRTLILNI--SKKYEHDS 300

Query: 301 TSSSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLAL 360
           ++S +KDLL  I++ + D P   A  + ++FIVDNCK+IYFAGHE+T+  A W LMLLA 
Sbjct: 301 STSVNKDLLHSIIQGSKDGP--FASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLAS 360

Query: 361 HPEWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLG 420
           H EWQ R R E    C    LD     +LK ++MVI ETLRLYPPA+FVARE   + +LG
Sbjct: 361 HHEWQSRARVESLDICQGRPLDFDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLG 420

Query: 421 NVVVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLC 480
            + +PKG  +W  I   HR+  +WG  A++F P+RFANG+A ACK P  Y+PFG G R C
Sbjct: 421 GIDIPKGTNIWIPIAMAHRDPSVWGPSADKFDPDRFANGIAGACKPPHMYMPFGVGVRTC 480

Query: 481 LGKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
            G+N A+VELK+++SL++SKF F LSP Y HCP++R+ +EP  GV ++F+ L
Sbjct: 481 AGQNLAMVELKVVLSLLLSKFEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of Chy10G174820 vs. ExPASy Swiss-Prot
Match: Q5KQH7 (Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 2.0e-124
Identity = 254/566 (44.88%), Postives = 341/566 (60.25%), Query Frame = 0

Query: 6   MSGFMLVFPTAILAAVV--AILLHFYSYGAW----RRTAEVRRKLGAQGVTGPPP-SILY 65
           M  F + F  A L  VV  A++       AW    RR AEV R+   QG+ GPPP S L 
Sbjct: 1   MESFFVFFTAAALPVVVAAAVIAGLCITAAWLARPRRVAEVFRR---QGIDGPPPSSFLA 60

Query: 66  GNLPEMQKIQLQTAAMASPLHRASAIVA---------------HDYTSTLFPYFVEWRKQ 125
           GNLPEM+      A+ A+P        +                DY + +FPYF +WRK 
Sbjct: 61  GNLPEMKARVAAAASAAAPTADGEETASAGGGGGGRDFEKDGFDDYCTRIFPYFHKWRKA 120

Query: 126 YGPLYTYSTGTRQHLYANKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVW 185
           YG  Y Y    R  LY    EL+ ++    SL++GKP Y+ K   P+ G  V+++NGA W
Sbjct: 121 YGETYLYWLRRRPALYVTDPELIGEIGRCVSLDMGKPKYLQKGQEPLFGGGVLKANGACW 180

Query: 186 AVQRKVIAPEFFMDRVRAMAFHMVDAASCLINKWESRV----GDGTIEIDVDEDLRGFSA 245
           A QRKVIAPEF+M RVRAM   MVDAA  LI  WESR+    G    E+ VD DLR FS 
Sbjct: 181 ARQRKVIAPEFYMARVRAMVQLMVDAAQPLIASWESRIDAAGGAAAAEVVVDGDLRSFSF 240

Query: 246 DVISRACFGSSYEKGKEIFSKLRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKE 305
           DVISRACFGS Y +G+EIF +LR+L  L+ E S +F   S   + L   K++RI RL  E
Sbjct: 241 DVISRACFGSDYSRGREIFLRLRELSGLMSETSVIFSIPSL--RHLPTGKNRRIWRLTGE 300

Query: 306 IESLIWETVQQRQ----------KECSKTSSSDKDLLQLIMEATIDDPNIGAKDSSKNFI 365
           I SLI E V++R+           + +  S  ++D L  I+E      N G +    +F+
Sbjct: 301 IRSLIMELVRERRCAARAAREHGGKAAPPSPPERDFLGSIIE------NSGGQPRPDDFV 360

Query: 366 VDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIRSEFAQAC-PDGHLDPTA-----T 425
           VDNCK+IYFAGHE++AV ATW LMLLA HPEWQDR R+E  + C  DG   P A      
Sbjct: 361 VDNCKNIYFAGHETSAVTATWCLMLLAAHPEWQDRARAEVLEVCGGDGAAAPAAPDFDMV 420

Query: 426 SQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVCLWTLIPTLHREVEIWGE 485
           S++++V MV+ ETLRL+PP++FV RETF + QLG ++ PKG  L+  + T+H +V  WG 
Sbjct: 421 SRMRTVGMVVQETLRLFPPSSFVVRETFRDMQLGRLLAPKGTYLFVPVSTMHHDVAAWGP 480

Query: 486 DANEFKPERFANGVAKACKFPQA-YVPFGAGPRLCLGKNFALVELKIIISLIVSKFRFSL 529
            A  F P RF +GVA ACK PQA ++PFG G R CLG+N ALVE+K ++++++++F F+L
Sbjct: 481 TARLFDPSRFRDGVAAACKHPQASFMPFGLGARTCLGQNLALVEVKTLVAVVLARFEFTL 540

BLAST of Chy10G174820 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 2.1e-118
Identity = 229/523 (43.79%), Postives = 323/523 (61.76%), Query Frame = 0

Query: 18  LAAVVAILLHFYSYG-AWRRTAEVRRKLGAQGVTGPPPSILYGNLPEMQKIQLQTAAMAS 77
           L  V A  L  Y Y   W    +V  K   Q + GP PS  YGNL +M++     AA A+
Sbjct: 15  LCCVGACGLAVYLYHILWLVPQKVLAKFEDQKIGGPRPSFPYGNLADMRE-----AAAAA 74

Query: 78  PLHRASAI--------VAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKD 137
              RASA         + HDY   + PY+ +WRK+YGP++TYS G    L+ ++ ++V+D
Sbjct: 75  KAARASARRSGSGGGGIVHDYRPAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRD 134

Query: 138 LSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVD 197
           ++L  SL++GK  Y+     P+ G  +++SNG  W  QRK+IAPEFF+D+V+ M   MVD
Sbjct: 135 INLCVSLDLGKSSYLKATHEPLFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVD 194

Query: 198 AASCLINKWESRV--GDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQK 257
           +A  L+  WE RV    G  +I +D+D+R +SADVISR CFGSSY KGKEIF K+R+LQ+
Sbjct: 195 SAQPLLMSWEERVDRNGGITDIKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRELQQ 254

Query: 258 LICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLL 317
            + + + L   +    +F    ++K+   L K++  LI E V        K S  D++LL
Sbjct: 255 AVSKPNVLAEMTGL--RFFPSMRNKQAWELHKQVRKLILEIV--------KESGEDRNLL 314

Query: 318 QLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIR 377
             I+ +      +G  + ++NFIVDNCKSIYFAGHESTAV A W LMLL LHPEWQ+R+R
Sbjct: 315 SAILHSA-STSRVGIAE-AENFIVDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVR 374

Query: 378 SEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVC 437
            E  + C    +D  +  ++K+++MVI ETLRLYP  AFV+R+   E +LG V +PKGV 
Sbjct: 375 EEVHEVCRGQPVDSRSLQKMKNLTMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVN 434

Query: 438 LWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVE 497
           ++  + T+H + E+WG D  EF PERF++   +      +Y+PFGAG R CLG+ FA+ E
Sbjct: 435 IYIPVSTMHLDPELWGPDVKEFNPERFSDVRPQL----HSYLPFGAGARTCLGQGFAMAE 494

Query: 498 LKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           LKI+ISLIVSKF   LSP Y H P+ ++IVEP  GV +   ++
Sbjct: 495 LKILISLIVSKFVLKLSPHYQHSPTLKLIVEPELGVDLTLTKV 516

BLAST of Chy10G174820 vs. ExPASy TrEMBL
Match: A0A0A0KQQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G224130 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.1e-303
Identity = 518/524 (98.85%), Postives = 522/524 (99.62%), Query Frame = 0

Query: 6   MSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGNLPEMQ 65
           MSGF+LVFPTAI+ AVVAILLHFYSYGAWRRTAEVRRKL AQGVTGPPPSILYGNLPEMQ
Sbjct: 1   MSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGNLPEMQ 60

Query: 66  KIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVEL 125
           KIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVEL
Sbjct: 61  KIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVEL 120

Query: 126 VKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFH 185
           VKDLSLSN+LNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFH
Sbjct: 121 VKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFH 180

Query: 186 MVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQ 245
           MVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQ
Sbjct: 181 MVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQ 240

Query: 246 KLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDL 305
           KLICEDSFLFGYSSWSD+FLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDL
Sbjct: 241 KLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSSDKDL 300

Query: 306 LQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRI 365
           LQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRI
Sbjct: 301 LQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRI 360

Query: 366 RSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGV 425
           RSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGV
Sbjct: 361 RSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGV 420

Query: 426 CLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALV 485
           CLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALV
Sbjct: 421 CLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALV 480

Query: 486 ELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           ELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL
Sbjct: 481 ELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 524

BLAST of Chy10G174820 vs. ExPASy TrEMBL
Match: A0A1S3CNV5 (cytochrome P450 714A1-like OS=Cucumis melo OX=3656 GN=LOC103502912 PE=3 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.6e-294
Identity = 500/529 (94.52%), Postives = 516/529 (97.54%), Query Frame = 0

Query: 1   MEGYVMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGN 60
           MEGYV+SGF+LVFPTA++ AVV ILLHFY YGAWRRTA+VRRKL AQGVTGPPPSILYGN
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLH ASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSN+LNVGKPFYVT+KLAPILGQSVVRSNG+VWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AM FHMVDAASCLINKWE RVGDGT+EIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMGFHMVDAASCLINKWERRVGDGTVEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLI EDSFLFGYSSWSD+FL+PRKHK I RLEKEIES+IWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLISEDSFLFGYSSWSDRFLRPRKHKSIKRLEKEIESIIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATI+DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATINDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLD TAT QLKSV+MVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIP LHREVEIWGEDAN+FKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRM+VEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 529

BLAST of Chy10G174820 vs. ExPASy TrEMBL
Match: A0A5A7VH75 (Cytochrome P450 714A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00950 PE=3 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 7.4e-292
Identity = 498/529 (94.14%), Postives = 514/529 (97.16%), Query Frame = 0

Query: 1   MEGYVMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGN 60
           MEGYV+SGF+LVFPTA++ AVV ILLHFY YGAWRRTA+VRRKL AQGVTGPPPSILYGN
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLH ASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSN+LNVGKPFYVT+KLAPILGQSVVRSNG+VWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AM FHMVDAASCLINKWE RVGDGT+EIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMGFHMVDAASCLINKWERRVGDGTVEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLI EDSFLFGYSSW  +FL+PRKHK I RLEKEIES+IWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLISEDSFLFGYSSW--RFLRPRKHKSIKRLEKEIESIIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATI+DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATINDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLD TAT QLKSV+MVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIP LHREVEIWGEDAN+FKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRM+VEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 527

BLAST of Chy10G174820 vs. ExPASy TrEMBL
Match: A0A6J1K5V5 (cytochrome P450 714A1-like OS=Cucurbita maxima OX=3661 GN=LOC111490994 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.8e-238
Identity = 414/530 (78.11%), Postives = 463/530 (87.36%), Query Frame = 0

Query: 6   MSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGNLPEMQ 65
           M G   V   A+  AVVA+++HFY YG WRRTAE+RRKL AQGVTGPPPS+LYGNL EMQ
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFY-YGVWRRTAEIRRKLRAQGVTGPPPSLLYGNLQEMQ 60

Query: 66  KIQLQ-TAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVE 125
           +IQL+  AAM SP H AS IVAHDYTSTLFPYFV+WRKQYGPLYTYSTGTRQHLYAN V+
Sbjct: 61  EIQLEAAAAMVSPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHVD 120

Query: 126 LVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAF 185
           LVKDLSL+N L++GKP YVTKKLAPILG S+VR+NG VWA+QRK+IAPEFFMD+VR MA 
Sbjct: 121 LVKDLSLTNYLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDQVRVMAT 180

Query: 186 HMVDAASCLINKWESRVGDG---TIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKL 245
            M +AA+ ++ KWE R+G+G   T EIDVDEDLR FSADVISRACFGSSYEKGK IFSKL
Sbjct: 181 LMTEAAAAMLQKWEGRIGEGEGATAEIDVDEDLREFSADVISRACFGSSYEKGKRIFSKL 240

Query: 246 RDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSSS 305
           R LQKL+ E +FLFGY S SD+  Q  KH+RI +LEKEIESLIWETVQQRQKECSKT SS
Sbjct: 241 RVLQKLLSEGNFLFGYVSLSDRLFQQSKHRRIKKLEKEIESLIWETVQQRQKECSKT-SS 300

Query: 306 DKDLLQLIMEATIDDPNIGA--KDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 365
           DKDLLQLIMEA ++DP + A   +S K FIVDNCKSIYFAGHESTAVAATWSLMLLALHP
Sbjct: 301 DKDLLQLIMEAAMNDPTVAANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360

Query: 366 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 425
           EWQDRIRSEF QAC DG ++ T  SQLKSVSMV+ ETLRLYPPAAFVARETF+ET+LGNV
Sbjct: 361 EWQDRIRSEFGQACLDGRVNMTTISQLKSVSMVVQETLRLYPPAAFVARETFSETRLGNV 420

Query: 426 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 485
           V+PKG+ LWTLIPTLHREVEIWGE+ANEFKPERF NG+AKACKFPQAY+PFGAGPRLCLG
Sbjct: 421 VIPKGIYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQAYLPFGAGPRLCLG 480

Query: 486 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           KNFALV+LKI+ISLIVSKFRFSLSPEY H PSYRMIVEPANG+KI+F+R+
Sbjct: 481 KNFALVQLKIVISLIVSKFRFSLSPEYRHSPSYRMIVEPANGMKIIFKRV 528

BLAST of Chy10G174820 vs. ExPASy TrEMBL
Match: A0A6J1FRZ8 (cytochrome P450 714A1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447915 PE=3 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 6.2e-238
Identity = 417/531 (78.53%), Postives = 464/531 (87.38%), Query Frame = 0

Query: 6   MSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGNLPEMQ 65
           M G   V  TA+  AVVA+++HFY YG WRRTAE+RRKL AQGVTGP PS+LYGNLPEMQ
Sbjct: 1   MGGLGSVLFTAVAVAVVAVVVHFY-YGVWRRTAEIRRKLRAQGVTGPSPSLLYGNLPEMQ 60

Query: 66  KIQLQ--TAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKV 125
           +IQL+   AAMASP H AS IVAHDYTSTLFPYFV+WRKQYGPLYTYSTGTRQHLYAN V
Sbjct: 61  EIQLEAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHV 120

Query: 126 ELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMA 185
           +LVKDLSL+N L++GKP YVTKKLAPILG S+VR+NG VWA+QRK+IAPEFFMDRVR MA
Sbjct: 121 DLVKDLSLTNHLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDRVRVMA 180

Query: 186 FHMVDAASCLINKWESRV--GDG-TIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 245
             M +AA+ ++ KWE R+  GDG T EIDVDE LR FSADVISRACFGSSYEKGK IFSK
Sbjct: 181 ALMTEAAAAMLQKWEERIDEGDGATAEIDVDEALREFSADVISRACFGSSYEKGKRIFSK 240

Query: 246 LRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 305
           LR LQKL+ E +FLFGY S SD+  Q  KH+ I +LEKEIESLIWETVQQRQKECSKT S
Sbjct: 241 LRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRTIKKLEKEIESLIWETVQQRQKECSKT-S 300

Query: 306 SDKDLLQLIMEATIDDPNIGA--KDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALH 365
           SDKDLLQLIMEA ++DP +G+   +S K FIVDNCKSIYFAGHESTAVAATWSLMLLALH
Sbjct: 301 SDKDLLQLIMEAAMNDPTVGSNCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLALH 360

Query: 366 PEWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGN 425
           PEWQDRIRSEF QACPDG ++ TA SQLKS SMV+ ETLRLYPPAAFVARETF+ET+LGN
Sbjct: 361 PEWQDRIRSEFTQACPDGRVNMTAISQLKSASMVVQETLRLYPPAAFVARETFSETRLGN 420

Query: 426 VVVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCL 485
           VV+PKGV LWTLIPTLHREVEIWGE+AN+FKPERF NG+AKACKFPQAYVPFGAGPRLCL
Sbjct: 421 VVIPKGVYLWTLIPTLHREVEIWGENANKFKPERFVNGIAKACKFPQAYVPFGAGPRLCL 480

Query: 486 GKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           GKNFALV+LKI+ISLIVSKFRFSLS EY H PSYRMIVEPANG+KI+F+R+
Sbjct: 481 GKNFALVQLKIVISLIVSKFRFSLSSEYRHSPSYRMIVEPANGMKIIFKRV 529

BLAST of Chy10G174820 vs. NCBI nr
Match: XP_004146466.1 (cytochrome P450 714A1 [Cucumis sativus] >KAE8648060.1 hypothetical protein Csa_005795 [Cucumis sativus])

HSP 1 Score: 1054 bits (2725), Expect = 0.0
Identity = 523/529 (98.87%), Postives = 527/529 (99.62%), Query Frame = 0

Query: 1   MEGYVMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGN 60
           MEGYVMSGF+LVFPTAI+ AVVAILLHFYSYGAWRRTAEVRRKL AQGVTGPPPSILYGN
Sbjct: 1   MEGYVMSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSN+LNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLICEDSFLFGYSSWSD+FLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 529
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 529

BLAST of Chy10G174820 vs. NCBI nr
Match: XP_008465257.1 (PREDICTED: cytochrome P450 714A1-like [Cucumis melo])

HSP 1 Score: 1013 bits (2620), Expect = 0.0
Identity = 500/529 (94.52%), Postives = 516/529 (97.54%), Query Frame = 0

Query: 1   MEGYVMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGN 60
           MEGYV+SGF+LVFPTA++ AVV ILLHFY YGAWRRTA+VRRKL AQGVTGPPPSILYGN
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLH ASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSN+LNVGKPFYVT+KLAPILGQSVVRSNG+VWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AM FHMVDAASCLINKWE RVGDGT+EIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMGFHMVDAASCLINKWERRVGDGTVEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLI EDSFLFGYSSWSD+FL+PRKHK I RLEKEIES+IWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLISEDSFLFGYSSWSDRFLRPRKHKSIKRLEKEIESIIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATI+DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATINDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLD TAT QLKSV+MVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIP LHREVEIWGEDAN+FKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 529
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRM+VEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 529

BLAST of Chy10G174820 vs. NCBI nr
Match: KAA0067078.1 (cytochrome P450 714A1-like protein [Cucumis melo var. makuwa] >TYK26315.1 cytochrome P450 714A1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1004 bits (2597), Expect = 0.0
Identity = 498/529 (94.14%), Postives = 514/529 (97.16%), Query Frame = 0

Query: 1   MEGYVMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGN 60
           MEGYV+SGF+LVFPTA++ AVV ILLHFY YGAWRRTA+VRRKL AQGVTGPPPSILYGN
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPPSILYGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASPLH ASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 61  LPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120

Query: 121 NKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSN+LNVGKPFYVT+KLAPILGQSVVRSNG+VWAVQRKVIAPEFFMDRVR
Sbjct: 121 NKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRVR 180

Query: 181 AMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240
           AM FHMVDAASCLINKWE RVGDGT+EIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK
Sbjct: 181 AMGFHMVDAASCLINKWERRVGDGTVEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 241 LRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300
           LRDLQKLI EDSFLFGYSSW  +FL+PRKHK I RLEKEIES+IWETVQQRQKECSKTSS
Sbjct: 241 LRDLQKLISEDSFLFGYSSW--RFLRPRKHKSIKRLEKEIESIIWETVQQRQKECSKTSS 300

Query: 301 SDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360
           SDKDLLQLIMEATI+DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE
Sbjct: 301 SDKDLLQLIMEATINDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPE 360

Query: 361 WQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVV 420
           WQDRIRSEFAQACPDGHLD TAT QLKSV+MVIHETLRLYPPAAFVARETFAETQLGNVV
Sbjct: 361 WQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQLGNVV 420

Query: 421 VPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480
           VPKGVCLWTLIP LHREVEIWGEDAN+FKPERFANGVAKACKFPQAYVPFGAGPRLCLGK
Sbjct: 421 VPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPRLCLGK 480

Query: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 529
           NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRM+VEPANGVKIVFQRL
Sbjct: 481 NFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 527

BLAST of Chy10G174820 vs. NCBI nr
Match: XP_038893045.1 (cytochrome P450 714A1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 466/530 (87.92%), Postives = 493/530 (93.02%), Query Frame = 0

Query: 1   MEGYVMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGN 60
           MEG ++ GF+ VFP A+   VVAIL+H Y YGAWRRTAEVRRKL AQGVTGPPPSILYGN
Sbjct: 6   MEGSIVCGFISVFPVAVTVTVVAILVHLYFYGAWRRTAEVRRKLRAQGVTGPPPSILYGN 65

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASP HRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 66  LPEMQKIQLQTAAMASPPHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 125

Query: 121 NKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSN+LN+GKPFYVTKKLAPILG S+VRSNGA+WAVQRK+IAPEFFMDRVR
Sbjct: 126 NKVELVKDLSLSNNLNLGKPFYVTKKLAPILGHSIVRSNGAIWAVQRKIIAPEFFMDRVR 185

Query: 181 AMAFHMVDAASCLINKWESRVGDG-TIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFS 240
           AMA  M DAAS L+ KWESR+GDG ++EI+VDEDLRGFSADVISRACFGSSYEKGKEIFS
Sbjct: 186 AMAVLMADAASSLVEKWESRIGDGASVEIEVDEDLRGFSADVISRACFGSSYEKGKEIFS 245

Query: 241 KLRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTS 300
           KLRDLQKLI E SFLFGYSS SD+FLQPRKHKRI +LEKEIESLIWETVQQRQKE SKTS
Sbjct: 246 KLRDLQKLISEGSFLFGYSSLSDRFLQPRKHKRIKKLEKEIESLIWETVQQRQKEFSKTS 305

Query: 301 SSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360
           SS+KDLLQLIMEAT +DPN+GA DSSKNFIVDNCKSIYFAGHESTAVAATW+LMLLALHP
Sbjct: 306 SSEKDLLQLIMEATTNDPNVGANDSSKNFIVDNCKSIYFAGHESTAVAATWTLMLLALHP 365

Query: 361 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 420
           EWQD IRSEFA ACPDG  D T  S LK+VSMV+HE LRLYPPAAFVARETFA+T+LGNV
Sbjct: 366 EWQDCIRSEFAHACPDGRPDTTVISNLKTVSMVVHEALRLYPPAAFVARETFADTRLGNV 425

Query: 421 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 480
           VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERF NG+AKACKFPQAYVPFGAGPRLCLG
Sbjct: 426 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFKNGIAKACKFPQAYVPFGAGPRLCLG 485

Query: 481 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 529
           KNFALV+LKIIISLIVSKFRFSLSPEY HCPSYRMIVEPANGVKI FQRL
Sbjct: 486 KNFALVQLKIIISLIVSKFRFSLSPEYCHCPSYRMIVEPANGVKIAFQRL 535

BLAST of Chy10G174820 vs. NCBI nr
Match: XP_038893046.1 (cytochrome P450 714A1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 931 bits (2407), Expect = 0.0
Identity = 464/530 (87.55%), Postives = 491/530 (92.64%), Query Frame = 0

Query: 1   MEGYVMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGN 60
           MEG ++ GF+ VFP A+   VVAIL+H Y YGAWRRTAEVRRKL AQGVTGPPPSILYGN
Sbjct: 6   MEGSIVCGFISVFPVAVTVTVVAILVHLYFYGAWRRTAEVRRKLRAQGVTGPPPSILYGN 65

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           LPEMQKIQLQTAAMASP HRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA
Sbjct: 66  LPEMQKIQLQTAAMASPPHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 125

Query: 121 NKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVR 180
           NKVELVKDLSLSN+LN+GKPFYVTKKLAPILG S+VRSNGA+WAVQRK+IAPEFFMDRVR
Sbjct: 126 NKVELVKDLSLSNNLNLGKPFYVTKKLAPILGHSIVRSNGAIWAVQRKIIAPEFFMDRVR 185

Query: 181 AMAFHMVDAASCLINKWESRVGDG-TIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFS 240
           AMA  M DAAS L+ KWESR+GDG ++EI+VDEDLRGFSADVISRACFGSSYEKGKEIFS
Sbjct: 186 AMAVLMADAASSLVEKWESRIGDGASVEIEVDEDLRGFSADVISRACFGSSYEKGKEIFS 245

Query: 241 KLRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTS 300
           KLRDLQKLI E SFLFGYSS   +FLQPRKHKRI +LEKEIESLIWETVQQRQKE SKTS
Sbjct: 246 KLRDLQKLISEGSFLFGYSSL--RFLQPRKHKRIKKLEKEIESLIWETVQQRQKEFSKTS 305

Query: 301 SSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360
           SS+KDLLQLIMEAT +DPN+GA DSSKNFIVDNCKSIYFAGHESTAVAATW+LMLLALHP
Sbjct: 306 SSEKDLLQLIMEATTNDPNVGANDSSKNFIVDNCKSIYFAGHESTAVAATWTLMLLALHP 365

Query: 361 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 420
           EWQD IRSEFA ACPDG  D T  S LK+VSMV+HE LRLYPPAAFVARETFA+T+LGNV
Sbjct: 366 EWQDCIRSEFAHACPDGRPDTTVISNLKTVSMVVHEALRLYPPAAFVARETFADTRLGNV 425

Query: 421 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 480
           VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERF NG+AKACKFPQAYVPFGAGPRLCLG
Sbjct: 426 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFKNGIAKACKFPQAYVPFGAGPRLCLG 485

Query: 481 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 529
           KNFALV+LKIIISLIVSKFRFSLSPEY HCPSYRMIVEPANGVKI FQRL
Sbjct: 486 KNFALVQLKIIISLIVSKFRFSLSPEYCHCPSYRMIVEPANGVKIAFQRL 533

BLAST of Chy10G174820 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 583.6 bits (1503), Expect = 1.6e-166
Identity = 294/499 (58.92%), Postives = 371/499 (74.35%), Query Frame = 0

Query: 34  WRRTAEVRRKLGAQGVTGPPPSILYGNLPEMQKIQLQTAAMASPLHRASAIVAHDYTSTL 93
           WR    +RRKL  QGV GPPPS+  GN+PEMQKIQ Q  +  S  +    I+AHDYTS+L
Sbjct: 36  WR----MRRKLTMQGVKGPPPSLFRGNVPEMQKIQSQIMS-NSKHYSGDNIIAHDYTSSL 95

Query: 94  FPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQ 153
           FPY   WRKQYG +YTYSTG +QHLY N  ELVK+L+ +N+LN+GK  YVTK+L  ILG+
Sbjct: 96  FPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQANTLNLGKVSYVTKRLKSILGR 155

Query: 154 SVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCLINKWE---SRVGDGTIEIDV 213
            V+ SNG  WA QR++IAPEFF+D+V+ M   +V++A  +++KWE    R G+   +I V
Sbjct: 156 GVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPMLSKWEEMMKREGEMVCDIIV 215

Query: 214 DEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICEDSFLFGYSSWSDKFLQPRKH 273
           DEDLR  SADVISRACFGSS+ KGKEIFSKLR LQK I  ++ LF  + ++D     +KH
Sbjct: 216 DEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITHNNILFSLNGFTDVVFGTKKH 275

Query: 274 --KRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQLIMEATID--DPNIGAKDSS- 333
              +I+ LE+ IESLIWETV++R++EC       KDL+QLI+E      D N+  K  S 
Sbjct: 276 GNGKIDELERHIESLIWETVKEREREC--VGDHKKDLMQLILEGARSSCDGNLEDKTQSY 335

Query: 334 KNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIRSEFAQACPDGHLDPTATSQ 393
           K+F+VDNCKSIYFAGHE++AVA +W LMLLAL+P WQ RIR E    C +G  D  + S 
Sbjct: 336 KSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRIRDEVFLHCKNGIPDADSISN 395

Query: 394 LKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVCLWTLIPTLHREVEIWGEDA 453
           LK+V+MVI ETLRLYPPAAFV+RE   +T+LGN+VVPKGVC+WTLIPTLHR+ EIWG DA
Sbjct: 396 LKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGVCIWTLIPTLHRDPEIWGADA 455

Query: 454 NEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVELKIIISLIVSKFRFSLSPE 513
           NEF PERF+ GV+KACK PQ++VPFG G RLCLGKNF ++ELK+++SLIVS+F F+LSP 
Sbjct: 456 NEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMMELKVLVSLIVSRFSFTLSPT 515

Query: 514 YHHCPSYRMIVEPANGVKI 525
           Y H P +RM+VEP +GV I
Sbjct: 516 YQHSPVFRMLVEPQHGVVI 527

BLAST of Chy10G174820 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 543.1 bits (1398), Expect = 2.5e-154
Identity = 267/518 (51.54%), Postives = 366/518 (70.66%), Query Frame = 0

Query: 17  ILAAVVAILLHFYSYGA---WRRTAEVRRKLGAQGVTGPPPSILYGNLPEMQKIQLQTAA 76
           ++  + ++  H Y       WR    +RR L  QGV GPPPSI  GN+ EMQ+IQ +   
Sbjct: 16  VIVGIFSVGYHVYGRAVVEQWR----MRRSLKLQGVKGPPPSIFNGNVSEMQRIQSEAKH 75

Query: 77  MASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSN 136
            +        I++HDY+S+LFP+F  WRKQYG +YTYSTG +QHLY N  E+VK+LS +N
Sbjct: 76  CS-----GDNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTN 135

Query: 137 SLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCL 196
           +LN+G+  ++TK+L PILG  ++ SNG  WA QR++IA EF  D+++ M   MV++A  +
Sbjct: 136 TLNLGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPM 195

Query: 197 INKWESRV---GDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDLQKLICE 256
           +NKWE  V   G+   +I VDEDL+  SADVI++ACFGSS+ KGK IFS +RDL   I +
Sbjct: 196 LNKWEEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITK 255

Query: 257 DSFLFGYSSWSDKFLQPRKH--KRINRLEKEIESLIWETVQQRQKECSKTSSSDKDLLQL 316
            S LF ++ ++D     +KH    I+ LE E+ES IWETV++R+ EC  T    KDL+QL
Sbjct: 256 RSVLFRFNGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHK--KDLMQL 315

Query: 317 IMEATID--DPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQDRIR 376
           I+E  +   D N+  K + + F+VDNCKSIYFAGH+STAV+ +W LMLLAL+P WQ +IR
Sbjct: 316 ILEGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIR 375

Query: 377 SEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVVPKGVC 436
            E   +C +G  D  +   LK+V+MVI ET+RLYPPA  V RE   + +LG++VVPKGVC
Sbjct: 376 DEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVC 435

Query: 437 LWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKNFALVE 496
           +WTLIP LHR+ EIWG DAN+FKPERF+ G++KACK+PQ+Y+PFG GPR C+GKNF ++E
Sbjct: 436 IWTLIPALHRDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMME 495

Query: 497 LKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKI 525
           +K+++SLIVSKF F+LSP Y H PS++++VEP +GV I
Sbjct: 496 VKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVI 522

BLAST of Chy10G174820 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 340.1 bits (871), Expect = 3.2e-93
Identity = 178/528 (33.71%), Postives = 302/528 (57.20%), Query Frame = 0

Query: 5   VMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGNLPEM 64
           ++   +++F T IL  +   +  +     W     +++ +  QGVTGP P  L GN+ E+
Sbjct: 5   ILKSLLVIFVTTILRVLYDTISCY-----WLTPRRIKKIMEQQGVTGPKPRPLTGNILEI 64

Query: 65  QKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVE 124
             +  Q+A+      +    + HD    L P++V W KQYG  +    GT   L   + E
Sbjct: 65  SAMVSQSAS------KDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETE 124

Query: 125 LVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAF 184
           L+K+L + ++   G+ +   +     +G+ ++ +NG  W  QR + AP F  +R++  A 
Sbjct: 125 LIKELLMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYAR 184

Query: 185 HMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSKLRDL 244
           HMV+  S L+ +    VG+G  E+++ E++   +AD+ISR  FGSS+EKGKE+F+ L  L
Sbjct: 185 HMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVL 244

Query: 245 QKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQ--KECSKTSSSD 304
           Q+   + +    +     +FL  + ++ I  L+KE+E L+ E +Q R+   E  ++S+  
Sbjct: 245 QRRCAQATRHLCFP--GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHG 304

Query: 305 KDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHPEWQ 364
            DLL L++     D N    +++   I+D CK+ +FAGHE+TA+  TW+ MLLA +P WQ
Sbjct: 305 DDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQ 364

Query: 365 DRIRSEFAQAC-PDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNVVV 424
           +++R E  +    +G       S+L S+S VI+E+LRLYPPA  + R  F + +LG++ +
Sbjct: 365 EKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTI 424

Query: 425 PKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLGKN 484
           PKG+ +W  +  +H   E+WG+DAN+F PERF     +     + ++PF AGPR C+G+ 
Sbjct: 425 PKGLSIWIPVLAIHHSEELWGKDANQFNPERFG---GRPFASGRHFIPFAAGPRNCIGQQ 484

Query: 485 FALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           FAL+E KII++ ++SKF F++S  Y H P   + ++P  GV+++ + L
Sbjct: 485 FALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Chy10G174820 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 336.3 bits (861), Expect = 4.6e-92
Identity = 175/494 (35.43%), Postives = 287/494 (58.10%), Query Frame = 0

Query: 40  VRRKLGAQGVTGPPPSILYGNLPEMQKIQLQTAAM-ASPLHRASAIVAHDYTSTLFPYFV 99
           +++ +  QG+TGP P +L GN+ ++ K+   +A+   S +H       H+    L P++V
Sbjct: 36  IKKFMERQGITGPKPRLLTGNIIDISKMLSHSASNDCSSIH-------HNIVPRLLPHYV 95

Query: 100 EWRKQYGPLYTYSTGTRQHLYANKVELVKDLSLSNSLNVGKPFYVTKKLAPILGQSVVRS 159
            W KQYG  +    GT   L   + E++K+L   ++   GK +   +     +G+ ++ +
Sbjct: 96  SWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSWLQQQGTKGFIGRGLLMA 155

Query: 160 NGAVWAVQRKVIAPEFFMDRVRAMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFS 219
           NG  W  QR + AP F  DR++  A HMV+    +  +    VG+   E+++ E++R  +
Sbjct: 156 NGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRKEVGE---EVEIGEEMRRLT 215

Query: 220 ADVISRACFGSSYEKGKEIFSKLRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEK 279
           AD+ISR  FGSS +KGKE+FS L  LQ+L  + +    +     +FL  + ++ I  L+ 
Sbjct: 216 ADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFP--GSRFLPSKYNREIKSLKT 275

Query: 280 EIESLIWETVQQRQK--ECSKTSSSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSI 339
           E+E L+ E +  R+   E  ++SS   DLL L++     + N    + +   I+D CK+ 
Sbjct: 276 EVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKN----NLNVQMIMDECKTF 335

Query: 340 YFAGHESTAVAATWSLMLLALHPEWQDRIRSEFAQAC-PDGHLDPTATSQLKSVSMVIHE 399
           +F GHE+T++  TW+LMLLA +P WQD +R E  Q C  DG       S L S++ VI+E
Sbjct: 336 FFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINE 395

Query: 400 TLRLYPPAAFVARETFAETQLGNVVVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFAN 459
           +LRLYPPA  + R  F + +LG++++PKG+ +W  +  +H   E+WGEDANEF PERF  
Sbjct: 396 SLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERF-- 455

Query: 460 GVAKACKFPQAYVPFGAGPRLCLGKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMI 519
              ++    + ++PF AGPR C+G+ FA++E KII++++VSKF F++S  Y H P   + 
Sbjct: 456 -TTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLT 510

Query: 520 VEPANGVKIVFQRL 530
           ++P  GV++V + L
Sbjct: 516 IKPKYGVQLVLKPL 510

BLAST of Chy10G174820 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 320.5 bits (820), Expect = 2.6e-87
Identity = 166/530 (31.32%), Postives = 282/530 (53.21%), Query Frame = 0

Query: 1   MEGYVMSGFMLVFPTAILAAVVAILLHFYSYGAWRRTAEVRRKLGAQGVTGPPPSILYGN 60
           ME + MS    +F    +  +  + L  +    W      ++KL   G  GP PS  +GN
Sbjct: 1   MEFFEMSRVWYIFFKVFVVVICLMFLKLF-LRCWIWPVRAQKKLRGNGFVGPAPSFPFGN 60

Query: 61  LPEMQKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYA 120
           L +M+K+++  A++     ++S I+ HD  S   P+F  W+++YG ++ Y  G    +Y 
Sbjct: 61  LNDMKKLKM--ASVVVDNSKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYV 120

Query: 121 NKVELVKDLSLS-NSLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRV 180
              E +  +S      + GKP    K   P+ G  +V   G  W   R +I P F    +
Sbjct: 121 ADPEFLSVMSKGVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNL 180

Query: 181 RAMAFHMVDAASCLINKWESRVGDGTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFS 240
           + M   MV++ S ++++W  ++  G  E D++ ++ G + ++I++  FG + E G ++  
Sbjct: 181 KVMTNMMVESVSNMLDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLK 240

Query: 241 KLRDLQKLICEDSFLFGYSSWSDKFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTS 300
            LR +Q  +   +   G        L  ++  +   L  EI+ L+   + +R+   ++  
Sbjct: 241 NLRAVQFALFNSNRYVGVP--FSNILSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGD 300

Query: 301 SSDKDLLQLIMEATIDDPNIGAKDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360
               DLL ++++A     N  AK+     +VD CK+ +FAGHE+TA+A TW+ MLLA+HP
Sbjct: 301 DQGHDLLGMLLKAD-QKGNFTAKE-----LVDECKTFFFAGHETTALALTWTFMLLAIHP 360

Query: 361 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 420
           EWQD IR E  +   D  ++    + LK +S V++E LRLYPPA    R+   + ++   
Sbjct: 361 EWQDTIREEIREVIGDSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGR 420

Query: 421 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 480
           V+P G  +W  +  +H +VE+WG+D NEFKPERF   +   CK    Y+PFG G R+C+G
Sbjct: 421 VIPNGTNIWIDVVAMHHDVELWGDDVNEFKPERFDGNLHGGCKNKMGYMPFGFGGRMCIG 480

Query: 481 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 530
           +N   +E KI++SL++S+F  S+SP Y H P+Y + + P  G+ ++ + L
Sbjct: 481 RNLTTMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z792.3e-16558.92Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1[more]
Q6NKZ83.5e-15351.54Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1[more]
Q2QYH71.7e-13145.86Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... [more]
Q5KQH72.0e-12444.88Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 ... [more]
B6SSW82.1e-11843.79Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KQQ71.1e-30398.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G224130 PE=3 SV=1[more]
A0A1S3CNV51.6e-29494.52cytochrome P450 714A1-like OS=Cucumis melo OX=3656 GN=LOC103502912 PE=3 SV=1[more]
A0A5A7VH757.4e-29294.14Cytochrome P450 714A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1K5V52.8e-23878.11cytochrome P450 714A1-like OS=Cucurbita maxima OX=3661 GN=LOC111490994 PE=3 SV=1[more]
A0A6J1FRZ86.2e-23878.53cytochrome P450 714A1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447... [more]
Match NameE-valueIdentityDescription
XP_004146466.10.098.87cytochrome P450 714A1 [Cucumis sativus] >KAE8648060.1 hypothetical protein Csa_0... [more]
XP_008465257.10.094.52PREDICTED: cytochrome P450 714A1-like [Cucumis melo][more]
KAA0067078.10.094.14cytochrome P450 714A1-like protein [Cucumis melo var. makuwa] >TYK26315.1 cytoch... [more]
XP_038893045.10.087.92cytochrome P450 714A1-like isoform X1 [Benincasa hispida][more]
XP_038893046.10.087.55cytochrome P450 714A1-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G24910.11.6e-16658.92cytochrome P450, family 714, subfamily A, polypeptide 1 [more]
AT5G24900.12.5e-15451.54cytochrome P450, family 714, subfamily A, polypeptide 2 [more]
AT5G38450.13.2e-9333.71cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT1G67110.14.6e-9235.43cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT5G52400.12.6e-8731.32cytochrome P450, family 715, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 273..293
NoneNo IPR availablePANTHERPTHR24282:SF36CYTOCHROME P450 714A1-RELATEDcoord: 16..524
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 16..524
IPR001128Cytochrome P450PRINTSPR00385P450coord: 391..402
score: 49.19
coord: 339..356
score: 34.07
coord: 477..488
score: 45.17
coord: 468..477
score: 59.73
IPR001128Cytochrome P450PFAMPF00067p450coord: 92..524
e-value: 2.7E-77
score: 260.5
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 217..235
score: 23.31
coord: 101..120
score: 24.36
coord: 348..374
score: 25.36
coord: 467..477
score: 52.59
coord: 477..500
score: 28.89
coord: 328..345
score: 22.84
coord: 390..408
score: 27.16
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 34..529
e-value: 3.5E-104
score: 351.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 88..527
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 470..479

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy10G174820.1Chy10G174820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen