Chy10G173360 (gene) Cucumber (hystrix) v1

Overview


Name	Chy10G173360
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chrH10: 3808588 .. 3809649 (-)
RNA-Seq Expression	Chy10G173360
Synteny	Chy10G173360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAACCTCGACGAAATAAAGGAAGCCAAAGGTGTAAGCATGAGCTGTGTCAACACACTTGACTTCAGGATGCCTAAGGACAAGATAAGTCAACATTTGCAGGACTTCGGGAGGGGTGGATTTCCCCACGAGTGAAACTACAACAAGAGTCAATTAACCTTGTGTTAGTTGTAAATTTGAAGGAATTCAAGTAACATGAAATGAGAGGGTGACACACAATTTAAAAGGCAGAAACGAAACTAAAATGCTACGTATAAACATACAATTACTTCAGGCATATGACAAAAGTATAGTTATTAGCCTCTGCTCAGAATTCAGCAGAATTCCTCTTTAACCAAAAAGGCTCTCACCAGTTCAGGTATACACAATTCACATCACAGCACGTCCAGGTCCACATCCACTTCAGAATCACTATCTCTGTAATCTAAGATCATTACTTCATTGATCCTAACAATCTACTTTCGTGTGGTTAAAGTGAGATATTGCAAGAATATGGAGAAAAAATGACAGAGCTATCAAGGAAAATTATAAAGAACATATTGATGAGCTTTGGTGAAGGTTTTGAGAAGAAATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAACTACACACCTCCAGAAACGTTGGAAGACGATTATGAAGCAGAAGGACTTGGAATGCATACAGATATGAGCTGCGTGACCATTGTGTACCAAGATTATGAAATAGGTAGACATAAACCCATGTAAAGAATCAGTAGCACTGGTGGTGAATATTTGGGACTTGCTTCAAGATTGGAGCAACGACAGGCTGAGGTCGTCGGAGCACAGAGTGGTTTTGTGGCAGCCGGAGATCAATCGATTCTCGATTGCTTTCTTTTGGGGTTTTGAGGACGAGAAGGTGGTGTCAGCGATGGAGGATTTGGTCGAAGAGGGCGGTAAGAGGATGTATAAGCCATTTGTCTGCTCGGATTATTTGAAGTTCATGGAGAATGATGAAAGAGGGAAATTTGAGAAGGTTGGTTTCACCTCATCTATCGGATTGATGGGCGGAATGACGTGA

mRNA sequence

ATGGATAACCTCGACGAAATAAAGGAAGCCAAAGGTGTAGACATAAACCCATGTAAAGAATCAGTAGCACTGGTGGTGAATATTTGGGACTTGCTTCAAGATTGGAGCAACGACAGGCTGAGGTCGTCGGAGCACAGAGTGGTTTTGTGGCAGCCGGAGATCAATCGATTCTCGATTGCTTTCTTTTGGGGTTTTGAGGACGAGAAGGTGGTGTCAGCGATGGAGGATTTGGTCGAAGAGGGCGGTAAGAGGATGTATAAGCCATTTGTCTGCTCGGATTATTTGAAGTTCATGGAGAATGATGAAAGAGGGAAATTTGAGAAGGTTGGTTTCACCTCATCTATCGGATTGATGGGCGGAATGACGTGA

Coding sequence (CDS)

ATGGATAACCTCGACGAAATAAAGGAAGCCAAAGGTGTAGACATAAACCCATGTAAAGAATCAGTAGCACTGGTGGTGAATATTTGGGACTTGCTTCAAGATTGGAGCAACGACAGGCTGAGGTCGTCGGAGCACAGAGTGGTTTTGTGGCAGCCGGAGATCAATCGATTCTCGATTGCTTTCTTTTGGGGTTTTGAGGACGAGAAGGTGGTGTCAGCGATGGAGGATTTGGTCGAAGAGGGCGGTAAGAGGATGTATAAGCCATTTGTCTGCTCGGATTATTTGAAGTTCATGGAGAATGATGAAAGAGGGAAATTTGAGAAGGTTGGTTTCACCTCATCTATCGGATTGATGGGCGGAATGACGTGA

Protein sequence

MDNLDEIKEAKGVDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDEKVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFTSSIGLMGGMT*

Homology

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BLAST of Chy10G173360 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 3.9e-23
Identity = 57/103 (55.34%), Postives = 75/103 (72.82%), Query Frame = 0


Query: 13  VDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEI--NRFSIAFFWGFEDEKV 72
           +DINP  E  ALVVN+ DLL  W+N RLRSS+HRV+L +     NRFS+AFFW F+D KV
Sbjct: 214 MDINPKDE--ALVVNVGDLLHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKV 273

Query: 73  VSAMEDLVEE-GGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 113
           V A +++V    G R+++ F C DYL+F E++E+GKFEKVG T
Sbjct: 274 VFAPDEVVGGCEGMRVFRSFKCGDYLRFRESNEKGKFEKVGDT 314

[Close]

BLAST of Chy10G173360 vs. ExPASy Swiss-Prot
Match: G9M9M0 (Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 7.8e-08
Identity = 35/90 (38.89%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 23  ALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDEKVVSAMEDLVEEGG 82
           A+V+N+ D LQ  SN R +S EHRV+      NR S+  F       V+  + +L+E G 
Sbjct: 267 AIVINVGDALQILSNGRYKSIEHRVIA-NGSNNRISVPIFVNPRPNDVIGPLPELLESGE 326

Query: 83  KRMYKPFVCSDYLK-FMENDERGKFEKVGF 112
           K +YK  + SDY+K F      GK E V F
Sbjct: 327 KAVYKNVLYSDYVKHFFRKAHDGK-ETVDF 354

[Close]

BLAST of Chy10G173360 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 7.8e-08
Identity = 33/86 (38.37%), Postives = 48/86 (55.81%), Query Frame = 0


Query: 21  SVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDEKVVSAMEDLVEE 80
           S +LV+NI D +Q  SN R +S EHR VL     NR S+  F   + E V+  + +++E 
Sbjct: 269 SGSLVINIGDAMQIMSNGRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIEN 328

Query: 81  GGKRMYKPFVCSDYLK-FMENDERGK 106
           G K +YK  + +DY+K F      GK
Sbjct: 329 GEKPVYKDILYTDYVKHFFRKAHDGK 353

[Close]

BLAST of Chy10G173360 vs. ExPASy Swiss-Prot
Match: G9M9M1 (Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.0e-07
Identity = 34/90 (37.78%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 23  ALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDEKVVSAMEDLVEEGG 82
           A+V+N+ D LQ  SN R +S EHRV+      NR S+  F       ++  + +L+E G 
Sbjct: 267 AIVINVGDALQILSNGRYKSIEHRVIA-NGSNNRISVPIFVNPRPNDIIGPLPELLESGE 326

Query: 83  KRMYKPFVCSDYLK-FMENDERGKFEKVGF 112
           K +YK  + SDY+K F      GK E V F
Sbjct: 327 KAVYKNVLYSDYVKHFFRKAHDGK-ETVDF 354

[Close]

BLAST of Chy10G173360 vs. ExPASy Swiss-Prot
Match: G9M9M2 (Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.0e-07
Identity = 34/90 (37.78%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 23  ALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDEKVVSAMEDLVEEGG 82
           A+V+N+ D LQ  SN R +S EHRV+      NR S+  F       ++  + +L+E G 
Sbjct: 267 AIVINVGDALQILSNGRYKSIEHRVIA-NGSNNRISVPIFVNPRPNDIIGPLPELLESGE 326

Query: 83  KRMYKPFVCSDYLK-FMENDERGKFEKVGF 112
           K +YK  + SDY+K F      GK E V F
Sbjct: 327 KAVYKNVLYSDYVKHFFRKAHDGK-ETVDF 354

[Close]

BLAST of Chy10G173360 vs. ExPASy TrEMBL
Match: A0A0A0KB92 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G004480 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.9e-45
Identity = 91/104 (87.50%), Postives = 95/104 (91.35%), Query Frame = 0


Query: 9   EAKGVDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDE 68
           E K VDINPC+ES ALVVNI DLLQ WSNDR+RSSEHRVVL +P INRFSIAFFWGFEDE
Sbjct: 211 EGKWVDINPCEESEALVVNIGDLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDE 270

Query: 69  KVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 113
           KVVSA EDLVEEGG+RMYKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 271 KVVSAPEDLVEEGGERMYKPFVCSDYLKFRENDERGKFEKVGFT 314

[Close]

BLAST of Chy10G173360 vs. ExPASy TrEMBL
Match: A0A5D3BVZ1 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001070 PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.4e-44
Identity = 90/104 (86.54%), Postives = 94/104 (90.38%), Query Frame = 0


Query: 9   EAKGVDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDE 68
           E K VDINPC+ES ALVVNI DLLQ WSNDRLRSSEHRVVL +P INRFSIAFFWGFEDE
Sbjct: 211 EGKWVDINPCEESEALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDE 270

Query: 69  KVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 113
           KVVSA ++LV EGGKRMYKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 271 KVVSAPKELVGEGGKRMYKPFVCSDYLKFRENDERGKFEKVGFT 314

[Close]

BLAST of Chy10G173360 vs. ExPASy TrEMBL
Match: A0A1S3C8M6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LOC103497717 PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.4e-44
Identity = 90/104 (86.54%), Postives = 94/104 (90.38%), Query Frame = 0


Query: 9   EAKGVDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDE 68
           E K VDINPC+ES ALVVNI DLLQ WSNDRLRSSEHRVVL +P INRFSIAFFWGFEDE
Sbjct: 211 EGKWVDINPCEESEALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDE 270

Query: 69  KVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 113
           KVVSA ++LV EGGKRMYKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 271 KVVSAPKELVGEGGKRMYKPFVCSDYLKFRENDERGKFEKVGFT 314

[Close]

BLAST of Chy10G173360 vs. ExPASy TrEMBL
Match: A0A6J1KY83 (gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 6.2e-40
Identity = 85/111 (76.58%), Postives = 95/111 (85.59%), Query Frame = 0


Query: 6   EIKEAKG--VDINPC--KESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAF 65
           ++K + G  VDI PC   ES ALVVNI DLLQ WSND+LRSSEHRVVL +P INRFSIAF
Sbjct: 206 QVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWSNDKLRSSEHRVVLRRPAINRFSIAF 265

Query: 66  FWGFEDEKVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 113
           FWGFEDEKVVSA E++V +GGK++YKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 266 FWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLKFRENDERGKFEKVGFT 316

[Close]

BLAST of Chy10G173360 vs. ExPASy TrEMBL
Match: A0A6J1H632 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111460359 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 8.1e-40
Identity = 87/117 (74.36%), Postives = 95/117 (81.20%), Query Frame = 0


Query: 6   EIKEAKG--VDINPCKE--------SVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEIN 65
           ++K + G  VDI PC E        S ALVVNI DLLQ WSNDRLRSSEHRVVL +P IN
Sbjct: 206 QVKSSAGDWVDIAPCDESESESESDSEALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAIN 265

Query: 66  RFSIAFFWGFEDEKVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 113
           RFSIAFFWGFEDEKVVSA E++V +GGKR+YKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 266 RFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFVCSDYLKFRENDERGKFEKVGFT 322

[Close]

BLAST of Chy10G173360 vs. NCBI nr
Match: XP_004138503.2 (gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN45657.1 hypothetical protein Csa_005484 [Cucumis sativus])

HSP 1 Score: 188 bits (477), Expect = 1.81e-56
Identity = 91/104 (87.50%), Postives = 95/104 (91.35%), Query Frame = 0


Query: 9   EAKGVDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDE 68
           E K VDINPC+ES ALVVNI DLLQ WSNDR+RSSEHRVVL +P INRFSIAFFWGFEDE
Sbjct: 211 EGKWVDINPCEESEALVVNIGDLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDE 270

Query: 69  KVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 112
           KVVSA EDLVEEGG+RMYKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 271 KVVSAPEDLVEEGGERMYKPFVCSDYLKFRENDERGKFEKVGFT 314

[Close]

BLAST of Chy10G173360 vs. NCBI nr
Match: XP_008458231.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo] >TYK03012.1 putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa])

HSP 1 Score: 185 bits (469), Expect = 3.07e-55
Identity = 90/104 (86.54%), Postives = 94/104 (90.38%), Query Frame = 0


Query: 9   EAKGVDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDE 68
           E K VDINPC+ES ALVVNI DLLQ WSNDRLRSSEHRVVL +P INRFSIAFFWGFEDE
Sbjct: 211 EGKWVDINPCEESEALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDE 270

Query: 69  KVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 112
           KVVSA ++LV EGGKRMYKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 271 KVVSAPKELVGEGGKRMYKPFVCSDYLKFRENDERGKFEKVGFT 314

[Close]

BLAST of Chy10G173360 vs. NCBI nr
Match: XP_038875412.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 172 bits (435), Expect = 5.57e-50
Identity = 86/112 (76.79%), Postives = 93/112 (83.04%), Query Frame = 0


Query: 9   EAKGVDINPCKESV--------ALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIA 68
           E K VDI+PC+ES         ALVVNI DLLQ W NDRLRSSEHRVVL +P INRFSIA
Sbjct: 211 EGKWVDIDPCEESELESKSESEALVVNIGDLLQAWGNDRLRSSEHRVVLRRPAINRFSIA 270

Query: 69  FFWGFEDEKVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 112
           FFWGFEDEKVVSA E+LV +GG+R+YKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 271 FFWGFEDEKVVSAPEELVGDGGERLYKPFVCSDYLKFRENDERGKFEKVGFT 322

[Close]

BLAST of Chy10G173360 vs. NCBI nr
Match: XP_023006611.1 (gibberellin 20-oxidase-like protein [Cucurbita maxima])

HSP 1 Score: 170 bits (431), Expect = 1.91e-49
Identity = 85/111 (76.58%), Postives = 95/111 (85.59%), Query Frame = 0


Query: 6   EIKEAKG--VDINPC--KESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAF 65
           ++K + G  VDI PC   ES ALVVNI DLLQ WSND+LRSSEHRVVL +P INRFSIAF
Sbjct: 206 QVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWSNDKLRSSEHRVVLRRPAINRFSIAF 265

Query: 66  FWGFEDEKVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 112
           FWGFEDEKVVSA E++V +GGK++YKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 266 FWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLKFRENDERGKFEKVGFT 316

[Close]

BLAST of Chy10G173360 vs. NCBI nr
Match: KAG6575175.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 170 bits (430), Expect = 3.13e-49
Identity = 87/117 (74.36%), Postives = 95/117 (81.20%), Query Frame = 0


Query: 6   EIKEAKG--VDINPCKESV--------ALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEIN 65
           ++K + G  VDI PC ES         ALVVNI DLLQ WSNDRLRSSEHRVVL +P IN
Sbjct: 206 QVKSSAGDWVDIAPCDESESESESDSEALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAIN 265

Query: 66  RFSIAFFWGFEDEKVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 112
           RFSIAFFWGFEDEKVVSA E++V +GGKR+YKPFVCSDYLKF ENDERGKFEKVGFT
Sbjct: 266 RFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFVCSDYLKFRENDERGKFEKVGFT 322

[Close]

BLAST of Chy10G173360 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 132.5 bits (332), Expect = 2.3e-31
Identity = 65/105 (61.90%), Postives = 81/105 (77.14%), Query Frame = 0


Query: 8   KEAKGVDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFED 67
           KE K +DINPC +   LVVNI DL+Q WSN RLRSSEHRVVL +  +NR S+AFF  FED
Sbjct: 90  KEGKWIDINPCND--FLVVNIGDLMQAWSNGRLRSSEHRVVL-RKLVNRVSLAFFLCFED 149

Query: 68  EKVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 113
           EKV+ A +++V EG +R YK F CS+YLKF +++E GKFEK+GFT
Sbjct: 150 EKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSNEEGKFEKIGFT 191

[Close]

BLAST of Chy10G173360 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 132.5 bits (332), Expect = 2.3e-31
Identity = 65/105 (61.90%), Postives = 81/105 (77.14%), Query Frame = 0


Query: 8   KEAKGVDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFED 67
           KE K +DINPC +   LVVNI DL+Q WSN RLRSSEHRVVL +  +NR S+AFF  FED
Sbjct: 208 KEGKWIDINPCND--FLVVNIGDLMQAWSNGRLRSSEHRVVL-RKLVNRVSLAFFLCFED 267

Query: 68  EKVVSAMEDLVEEGGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 113
           EKV+ A +++V EG +R YK F CS+YLKF +++E GKFEK+GFT
Sbjct: 268 EKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSNEEGKFEKIGFT 309

[Close]

BLAST of Chy10G173360 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 2.7e-24
Identity = 57/103 (55.34%), Postives = 75/103 (72.82%), Query Frame = 0


Query: 13  VDINPCKESVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEI--NRFSIAFFWGFEDEKV 72
           +DINP  E  ALVVN+ DLL  W+N RLRSS+HRV+L +     NRFS+AFFW F+D KV
Sbjct: 214 MDINPKDE--ALVVNVGDLLHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKV 273

Query: 73  VSAMEDLVEE-GGKRMYKPFVCSDYLKFMENDERGKFEKVGFT 113
           V A +++V    G R+++ F C DYL+F E++E+GKFEKVG T
Sbjct: 274 VFAPDEVVGGCEGMRVFRSFKCGDYLRFRESNEKGKFEKVGDT 314

[Close]

BLAST of Chy10G173360 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 5.6e-09
Identity = 33/86 (38.37%), Postives = 48/86 (55.81%), Query Frame = 0


Query: 21  SVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDEKVVSAMEDLVEE 80
           S +LV+NI D +Q  SN R +S EHR VL     NR S+  F   + E V+  + +++E 
Sbjct: 269 SGSLVINIGDAMQIMSNGRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIEN 328

Query: 81  GGKRMYKPFVCSDYLK-FMENDERGK 106
           G K +YK  + +DY+K F      GK
Sbjct: 329 GEKPVYKDILYTDYVKHFFRKAHDGK 353

[Close]

BLAST of Chy10G173360 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.6e-08
Identity = 31/89 (34.83%), Postives = 50/89 (56.18%), Query Frame = 0


Query: 21  SVALVVNIWDLLQDWSNDRLRSSEHRVVLWQPEINRFSIAFFWGFEDEKVVSAMEDLVEE 80
           S AL++N+ + +Q W+ND   S+EHRVV+      RFSI FF+    E  +  +E+L+ E
Sbjct: 256 SDALIINMGNCIQVWTNDEYWSAEHRVVV-NTSKERFSIPFFFFPSHEANIEPLEELISE 315

Query: 81  GGKRMYKPFVCSDYLKFMENDERGKFEKV 110
                YK +   ++ KF  +  R  F+K+
Sbjct: 316 ENPPCYKKY---NWGKFFVSRNRSDFKKL 340

The following BLAST results are available for this feature:

BLAST of Chy10G173360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	3.9e-23	55.34	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
G9M9M0	7.8e-08	38.89	Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 ...	[more]
Q9C899	7.8e-08	38.37	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S...	[more]
G9M9M1	1.0e-07	37.78	Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 ...	[more]
G9M9M2	1.0e-07	37.78	Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 ...	[more]

BLAST of Chy10G173360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KB92	2.9e-45	87.50	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A5D3BVZ1	2.4e-44	86.54	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3C8M6	2.4e-44	86.54	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1KY83	6.2e-40	76.58	gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 ...	[more]
A0A6J1H632	8.1e-40	74.36	gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11146035...	[more]

BLAST of Chy10G173360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138503.2	1.81e-56	87.50	gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN45657.1 hypothetical p...	[more]
XP_008458231.1	3.07e-55	86.54	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo] >TY...	[more]
XP_038875412.1	5.57e-50	76.79	gibberellin 20-oxidase-like protein [Benincasa hispida]	[more]
XP_023006611.1	1.91e-49	76.58	gibberellin 20-oxidase-like protein [Cucurbita maxima]	[more]
KAG6575175.1	3.13e-49	74.36	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro...	[more]

BLAST of Chy10G173360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.2	2.3e-31	61.90	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.1	2.3e-31	61.90	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	2.7e-24	55.34	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G55290.1	5.6e-09	38.37	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.1	1.6e-08	34.83	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 11..62 e-value: 2.6E-5 score: 24.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 6..114 e-value: 8.9E-21 score: 76.5
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 10..116
None	No IPR available	PANTHER	PTHR47990:SF52	GIBBERELLIN 3-BETA-DIOXYGENASE 4	coord: 10..116
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 12..101

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy10G173360.1	Chy10G173360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0047927	gibberellin-44 dioxygenase activity
molecular_function	GO:0046872	metal ion binding