CcUC10G190610 (gene) Watermelon (PI 537277) v1

BLAST of CcUC10G190610 vs. NCBI nr
Match: XP_038904656.1 (DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida] >XP_038904657.1 DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1608.2 bits (4163), Expect = 0.0e+00
Identity = 841/939 (89.56%), Postives = 858/939 (91.37%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGR  SD+C
Sbjct: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKH 120
           S+NGQSSNNQKRMT LPKTSPMRSEIGS KASKS I+EP VKPLGT+LKVSDSM SR K 
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120

Query: 121 LVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDSD 180
           LVN +VK+ENV++  T +NSDSEDDKPLSIRLKGNS+QPNKG  PTVPKNKVKK SGDSD
Sbjct: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180

Query: 181 DEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPS 240
           DEIPLSSKFQMKS SG LG KQHGFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP+
Sbjct: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEVDD-DDDIPISQRMKKISAPVNKSS 300
           ADSH+ SQ S KKPKLSDSSTPLSSK VSLKAKQEVDD DDDIPISQRMKK+SAPVNKSS
Sbjct: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300

Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
           SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIY 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK     
Sbjct: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK----- 480

Query: 481 HFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRGR 540
                                                        VGNFRVEPPGLFRGR
Sbjct: 481 ---------------------------------------------VGNFRVEPPGLFRGR 540

Query: 541 GEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQK 600
           GEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQK
Sbjct: 541 GEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQK 600

Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLI 660
           EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKD TRRQIAVATYLI
Sbjct: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLI 660

Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
           DKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSIKYENTVEVELPVF
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPVF 720

Query: 721 KAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
           KAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD
Sbjct: 721 KAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780

Query: 781 GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
           GDV EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KELKIDLDRAK
Sbjct: 781 GDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAK 840

Query: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 900
           KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 889

Query: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
           PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of CcUC10G190610 vs. NCBI nr
Match: XP_008449103.1 (PREDICTED: DNA topoisomerase 1-like [Cucumis melo] >XP_008449104.1 PREDICTED: DNA topoisomerase 1-like [Cucumis melo])

HSP 1 Score: 1587.4 bits (4109), Expect = 0.0e+00
Identity = 832/940 (88.51%), Postives = 856/940 (91.06%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GR  SD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
           S+NGQSSNNQKRMT LPKTSPMRSEIGS KA KSS IQEPL K LG +LK SDSM SRPK
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 HLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDS 180
           +LVNA+VKEENV++NP+ +NSDSEDDKPLSIRLKGNSNQPNKG   T+PKNKVKK SGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK ASG +G KQ GFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 SADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEV-DDDDDIPISQRMKKISAPVNKS 300
           + DSHS SQFS KKPKLSDSS+PLSSK VSLKAKQEV DDDDDIPISQR+KK  A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK    
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK---- 480

Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRG 540
                                                         VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
           RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780

Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
           DG+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840

Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
           KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 889

Query: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
           DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of CcUC10G190610 vs. NCBI nr
Match: KAA0065816.1 (DNA topoisomerase 1-like [Cucumis melo var. makuwa] >TYJ96555.1 DNA topoisomerase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1576.2 bits (4080), Expect = 0.0e+00
Identity = 832/958 (86.85%), Postives = 856/958 (89.35%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GR  SD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
           S+NGQSSNNQKRMT LPKTSPMRSEIGS KA KSS IQEPL K LG +LK SDSM SRPK
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 HLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDS 180
           +LVNA+VKEENV++NP+ +NSDSEDDKPLSIRLKGNSNQPNKG   T+PKNKVKK SGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK ASG +G KQ GFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 SADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEV-DDDDDIPISQRMKKISAPVNKS 300
           + DSHS SQFS KKPKLSDSS+PLSSK VSLKAKQEV DDDDDIPISQR+KK  A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK    
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK---- 480

Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRG 540
                                                         VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
           RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780

Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
           DG+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840

Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
           KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900

Query: 901 DPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAMDVDPDFRF 939
           DPRITVAWCKRHEVPIEK                  IFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAMDVDPDFRF 907

BLAST of CcUC10G190610 vs. NCBI nr
Match: XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1572.4 bits (4070), Expect = 0.0e+00
Identity = 823/939 (87.65%), Postives = 852/939 (90.73%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGRP SD C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKH 120
           S+NGQSSN QKRMT LPKTSPMR EIGS KASKSSIQEPLVK LGTTLKVSDSM SRPK 
Sbjct: 61  SANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDSD 180
           L NA+VKEEN +++PT DNSDSEDDKPLSIRLKGNSN P+KGP PTV KNK+KK S DSD
Sbjct: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPS 240
           DE+PLSSKFQMKSASG LGG+QHGFDEKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLP+
Sbjct: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEV-DDDDDIPISQRMKKISAPVNKSS 300
           ADSHSSSQ S KKPKLSDSSTPLS+  VSLKAKQEV DDDD IPISQRM K+SA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRM-KMSAAVNKSS 300

Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
            SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKIL+KGKPVDLTPEQEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIY 480
           QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK     
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK----- 480

Query: 481 HFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRGR 540
                                                        VGNFRVEPPGLFRGR
Sbjct: 481 ---------------------------------------------VGNFRVEPPGLFRGR 540

Query: 541 GEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQK 600
           GEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+ WKEVRHDNTVTWLAFWNDPIN K
Sbjct: 541 GEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPK 600

Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLI 660
           EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKDPTRRQIAVATYLI
Sbjct: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLI 660

Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
           DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720

Query: 721 KAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
           KAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD
Sbjct: 721 KAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780

Query: 781 GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
           G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KELKIDLDRAK
Sbjct: 781 GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAK 840

Query: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 900
           KGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 887

Query: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
           PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of CcUC10G190610 vs. NCBI nr
Match: XP_004149585.1 (DNA topoisomerase 1 alpha [Cucumis sativus] >XP_011650506.1 DNA topoisomerase 1 alpha [Cucumis sativus] >KGN56068.1 hypothetical protein Csa_010762 [Cucumis sativus])

HSP 1 Score: 1572.0 bits (4069), Expect = 0.0e+00
Identity = 824/940 (87.66%), Postives = 851/940 (90.53%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKAPMF DDDDEDNMPIVFKR+SASRQNQLDSEIKHSQ+QRL+KL GR  SD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
           S+NGQSSNNQKRMT LPKTSPMRSEIGS KAS+SS I EPL K LGT+LK S SM SRPK
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPK 120

Query: 121 HLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDS 180
            LVNA VKEENV++NP+ +NSDSEDDKPLSIRLKGN NQPNKG   TVPKNKVKK   DS
Sbjct: 121 SLVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADS 180

Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK ASG LG KQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 SADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEV-DDDDDIPISQRMKKISAPVNKS 300
           + DSHS SQF  KKPKL+DSS+PL+SK VSLKAKQEV DDDDDIPISQRMKK+SA VNKS
Sbjct: 241 NEDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK T+SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEK    
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEK---- 480

Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRG 540
                                                         VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
           RGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           FKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780

Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
           +G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 NGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840

Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
           KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 889

Query: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
           DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of CcUC10G190610 vs. ExPASy Swiss-Prot
Match: P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 1.7e-291
Identity = 577/990 (58.28%), Postives = 681/990 (68.79%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRPA 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    K+S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGS 120
           S V S NG         TP  KTS ++S + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKHLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGN----------SNQPNKGPGPT 180
             K      ++ E           DSEDD+PLS  L GN          S QP K     
Sbjct: 121 -QKDQSECKIEHE-----------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 VPKNKVKKPSGD-SDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDK 240
            P ++  +   D SDDE P+SS F+ K  SG  GG Q   DEKKPLV K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVLSSKRPLPSADSHSSSQFSMKKPKLSDSSTPLSSKPVS---------------- 300
           +    VL  KRPL    + S+ Q S+KK K+S S T +  K  S                
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300

Query: 301 LKAKQ--------EVDDDDDIPISQRMKKISAPVNKSSSSKQTVS-------------KV 360
           +KAKQ        + DDDDD+PIS+R K  S+  N SS+  + V               V
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360

Query: 361 VSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
           VS   +   K +KK  K SKY  S+K  PS+GDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420

Query: 421 YKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           YKGKPVDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480

Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFWKN 540
           PIY+W+  EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK              
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEK-------------- 540

Query: 541 LVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMGKL 600
                                               +GNFRVEPPGLFRGRGEHPKMGKL
Sbjct: 541 ------------------------------------IGNFRVEPPGLFRGRGEHPKMGKL 600

Query: 601 KRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFLAA 660
           K+RI P +IT+NIGKGAPIPECPI GE WKEV+HDNTVTWLAFW DPIN KEFKYVFL A
Sbjct: 601 KKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFLGA 660

Query: 661 SSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLALRAGN 720
            SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVATYLIDKLALRAGN
Sbjct: 661 GSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRAGN 720

Query: 721 EKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSG 780
           EKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ+G
Sbjct: 721 EKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAG 780

Query: 781 KSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIVT 840
           KS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KIV 
Sbjct: 781 KSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIVV 840

Query: 841 YQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKDA 900
           YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAKKGKPPL+ +
Sbjct: 841 YQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEGS 900

Query: 901 DGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCK 939
           DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWCK
Sbjct: 901 DGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWCK 913

BLAST of CcUC10G190610 vs. ExPASy Swiss-Prot
Match: Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 2.6e-289
Identity = 574/993 (57.80%), Postives = 692/993 (69.69%), Query Frame = 0

Query: 1   MAVETFGKAPMFD-----DDDDEDNMPIVFKRSS-----ASRQNQLDSEIKHSQSQRLNK 60
           MA E F K  + +     +D+DED++P+VFKR+S      +R + +++ +++S     + 
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIG--ST 60

Query: 61  LSGRPASDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKV 120
            S  P     +S  +S+++  R + +    P  S +      +S+++ PL        K 
Sbjct: 61  KSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSV-----QRSTLKSPLRDDRSVVAKE 120

Query: 121 SDSMGSRPKHLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPT---- 180
            +  G  P             +V+ +DD  DSEDDKPLS RLK +S +  K P  +    
Sbjct: 121 RNGFGKAP-------------SVSKSDD-EDSEDDKPLSARLKLDSKEVTKQPSSSGRGS 180

Query: 181 ----VPKNKVKKPSGDS--------DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPK 240
               V K+ + +P G S        D+  P+SS  Q K++ G    K    ++K+PLV  
Sbjct: 181 TQQAVQKSNM-RPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNN 240

Query: 241 VHQNG---STSRDKLQKPAVLSSKRPLPSADSHSSSQFSMKKPKLSDSSTPL-------- 300
           + +NG    T     Q PA    KRPL      SS+Q S+K+PKLS+ + P+        
Sbjct: 241 IDRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHI 300

Query: 301 ------------SSKPVSLKAKQEV------DDDDDIPISQRMKKISAPVNKSSSSKQTV 360
                       +SKP  L+A Q        D DD +PI+ RMK  S+  NKSSS+K + 
Sbjct: 301 SATQDAKGKNLDASKP--LRANQATVKEDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSS 360

Query: 361 SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGV 420
           SK+++SS +   +K  K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGV
Sbjct: 361 SKMIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGV 420

Query: 421 KILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDC 480
           KIL++GKPVDLTPEQEEVATMFAVM++T+Y  KPKF+ENFWNDWRKLLGKNH+I++LDDC
Sbjct: 421 KILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDC 480

Query: 481 DFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAF 540
           DFTPIY+WY  EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E+           
Sbjct: 481 DFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRER----------- 540

Query: 541 WKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRGRGEHPKM 600
                                                  +GNFRVEPPGLFRGRGEHPKM
Sbjct: 541 ---------------------------------------IGNFRVEPPGLFRGRGEHPKM 600

Query: 601 GKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVF 660
           GKLK+RIRP DITINIGK APIPECPIPGE WKEV+HDNTVTWLAFW+DPIN KEFKYVF
Sbjct: 601 GKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYVF 660

Query: 661 LAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLALR 720
           LAASSSLKGQSDKEKYEKAR L ++I +IR AYTKDF +KD T+RQIAVATYLIDKLALR
Sbjct: 661 LAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALR 720

Query: 721 AGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQF 780
           AGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  V+KAI QF
Sbjct: 721 AGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIGQF 780

Query: 781 QSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEK 840
           Q+GKS TDDLFD+LDT+KLN HLK+LM GLTAKVFRTYNASITLD ML++ T+DGDV EK
Sbjct: 781 QAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDVPEK 840

Query: 841 IVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPL 900
           +V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDRAKKG+ PL
Sbjct: 841 VVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGRTPL 900

Query: 901 KDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVA 939
             +DGKRKRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSKINY+DPRITVA
Sbjct: 901 MGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRITVA 911

BLAST of CcUC10G190610 vs. ExPASy Swiss-Prot
Match: P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 2.1e-233
Identity = 482/860 (56.05%), Postives = 579/860 (67.33%), Query Frame = 0

Query: 120 HLVNASVKEENVTVNPTDDNSDSEDDKPLSIR-----LKGNSNQPNKG-----PGP-TVP 179
           +++  ++KEE        ++ DS+DDKPLS +     L GNS+    G     P P T P
Sbjct: 16  NVMEKTLKEEG---QSRSNSEDSDDDKPLSHKLSSGALNGNSHHIRMGSNLSCPSPYTSP 75

Query: 180 KNKVKKPSGDSDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQK 239
           K ++ K     +DE+ LSS+ Q+   +GA   +    DE KPL             +L+ 
Sbjct: 76  KPRIIK---GPEDEMLLSSELQLN--AGASNAESSDSDESKPLA-----------SELED 135

Query: 240 PAVLSSKRPLPSADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEVDDDDDIPISQRM 299
           P+   +KRPL      +S     KK KLS+ S P + K +    ++E   DDD  IS R 
Sbjct: 136 PSSSLNKRPL--IQPKNSDPSPSKKAKLSEPSAPTNRK-LKTAEQEEAAADDDPSISNRN 195

Query: 300 KKISAPVNKSSSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTT 359
           KK + P +K S  K+     VS+S+ K        V  SK +K   +L    +GQ     
Sbjct: 196 KKSTTPASKVSDKKKRPD--VSASVNK--------VDFSKSLKVPLVLVKGRNGQ----P 255

Query: 360 LVHNGVIFPPPYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWND 419
           LVHNGVIFPP Y+ HGVK+LY+  PVDLTPEQEEVATMFAVM +T+YM KPKF+ENF +D
Sbjct: 256 LVHNGVIFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLETEYMTKPKFRENFMSD 315

Query: 420 WRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLK-QEEKYMWAI 479
           WRK+LG+ H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKKA+KE K + ++EKYMWA+
Sbjct: 316 WRKILGEKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIKEGKDETRKEKYMWAV 375

Query: 480 LDGVKEKVILIYHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGN 539
           +DG+  +                                                 +VGN
Sbjct: 376 VDGMSRE-------------------------------------------------KVGN 435

Query: 540 FRVEPPGLFRGRGEHPKMGKLKRRIRPS-DITINIGKGAPIPECPIPGESWKEVRHDNTV 599
           FRV    + +      K  ++K+ +    DITINIGK APIPE PIPGE WKE+RHDNTV
Sbjct: 436 FRVGTTRVVQRSWRASKDPQVKKNVYSQIDITINIGKDAPIPEPPIPGERWKEIRHDNTV 495

Query: 600 TWLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIR-NAYTKDFTSK 659
           TWLAFWNDPIN KEFKYVFLAASSSLKG+  +EKYEK+R LKDYI+ IR  AYTKDF SK
Sbjct: 496 TWLAFWNDPINPKEFKYVFLAASSSLKGKVTREKYEKSRKLKDYIEGIRAAAYTKDFASK 555

Query: 660 DPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDS 719
           D  +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+   PN+L+F+FLGKDS
Sbjct: 556 DSKKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVETKRPNILKFDFLGKDS 615

Query: 720 IKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNA 779
           I+Y+N VEVE  VF AI QF+SGK G DDLFD+LDT+KLNAHLK+LMPGLTAKVFRTYNA
Sbjct: 616 IRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLDTSKLNAHLKELMPGLTAKVFRTYNA 675

Query: 780 SITLDDM-----LNRGTKDGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEK 839
           SITLD+M     L+R TK GDVAEK+V YQHANKEVAIICNHQRTVSKSH AQM RLNEK
Sbjct: 676 SITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMVRLNEK 735

Query: 840 ISELRDILKELKIDLDRAKKGKPPLKDADGKRKRNLTPE--------------------- 899
           I EL+ +LKEL+ DL R  KGKPPLK++DGK KRNL  +                     
Sbjct: 736 IEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKRNLNLKRYKGKLLKLIQKLRKWSETRR 790

Query: 900 -ALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIF 939
            ++ KKI+QTN KIEK+ERD +TKEDLKTVALGTSKINYLDPRITVAWCKRH+VPIEKIF
Sbjct: 796 LSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHDVPIEKIF 790

BLAST of CcUC10G190610 vs. ExPASy Swiss-Prot
Match: Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.2e-142
Identity = 352/924 (38.10%), Postives = 498/924 (53.90%), Query Frame = 0

Query: 71  KRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKHLVNASVKEEN 130
           K++TP PK +P+  +  +    K  I  P  KP+ ++   SDS  S             +
Sbjct: 59  KKLTPTPKPAPVTPKPTAAVNGKKPI--PKKKPVDSS---SDSDSS-------------D 118

Query: 131 VTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDSDDEIPLSSKFQ 190
            + +   D+SDS+ D   S     +S+ P K P   +P     KPSG        S K  
Sbjct: 119 SSSDSDSDSSDSDSDSSSSDSSSSDSDVPKKKPISKIP----NKPSGTVSKTSKNSPKTT 178

Query: 191 MKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPSADSHSSSQFS 250
             + +     K       KP  PKV    S+S D     +  SS     S+DS S  +  
Sbjct: 179 TTTTTTTTTVK------SKP-APKVKPEQSSSDDSSSDSSDDSSDD--SSSDSSSEEEKP 238

Query: 251 MKKPKLSDSS----------TPLSSKPVSLKAKQEVDDDDDIPISQRMKKISAPVNKSSS 310
            KKP    ++          T  ++K   +K + EV  +     ++++KK  +  +    
Sbjct: 239 KKKPSKKPAAVKKEITVKKETTTTTKKTPVKKEVEVKKETKTTTTKKVKKEESD-DDGED 298

Query: 311 SKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPY 370
            K T  K  ++  +K  ++ +   ++ + V          D   KWTTL+HNG +FPP Y
Sbjct: 299 EKTTTKKKTTTRKRKLKEEEEGEEEEEEGVNKWWDKEDDEDDGTKWTTLIHNGPVFPPAY 358

Query: 371 QTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLL--GKNHV 430
             HG+K +Y GK V LTP+QEEVAT +A   +TD++ K  FK+NF+ +++ LL   +  +
Sbjct: 359 VPHGIKFMYDGKVVKLTPKQEEVATFYANYLETDHVKKQAFKDNFFKEFKGLLTSEQKEI 418

Query: 431 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 490
           ++ +D CDF+ I+   +T+KE++K  + EEK A   EK K +EK+ +A +DG KEK    
Sbjct: 419 VKKMDKCDFSHIHTHLKTKKEQRKARSKEEKDAETAEKKKIQEKHGFATIDGFKEK---- 478

Query: 491 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRG 550
                                                         VGN+ +EPPGLF G
Sbjct: 479 ----------------------------------------------VGNYMIEPPGLFLG 538

Query: 551 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 610
           RG+HPK G LK+RI PSD+T+NIG+G  IP  PIPGE WKE+ H+NTVTWLAFW + +N 
Sbjct: 539 RGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPSPIPGEKWKEIVHNNTVTWLAFWRESVN- 598

Query: 611 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 670
             FKYV+LA SS +KGQ+D++K+E AR LK+ I +IR  Y K F  +   +RQ+AVA YL
Sbjct: 599 GGFKYVWLAPSSKIKGQADRKKFEVARGLKNCIGDIRKGYQKSFNDESTEKRQLAVALYL 658

Query: 671 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 730
           ID LALR GNEK +D+ADTVGCC+L+VE++K    N +  +FLGKDS++Y NTV++    
Sbjct: 659 IDFLALRVGNEKGEDQADTVGCCSLRVEHIKLESNNTITLDFLGKDSMRYLNTVQIREDA 718

Query: 731 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR-GT 790
           +K +  F  GK  TD LFD L   +LN HLK  M GL+AKVFRTYNASITL   L++  T
Sbjct: 719 YKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKKQMTGLSAKVFRTYNASITLQKELDKMDT 778

Query: 791 KD-GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLD 850
           K+   + EKI+ Y   N+EVAI+CNHQ+   K+    M++++EKI ELRD+      DL 
Sbjct: 779 KEFQSIDEKILFYNRCNREVAILCNHQKAPPKNLAETMSKIDEKIQELRDLQ-----DLC 838

Query: 851 RAKKGKPP-------------------------LKDADGKR--------------KRNLT 910
           R K GK P                          KD D ++              K    
Sbjct: 839 RIKIGKTPKSQQEDIESREAERLPKLIQLAITNKKDKDKEKTDKEISFDVKAAFEKSRTL 893

Query: 911 PEA---LEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 939
           P+A   +++KI++   +I+K E     K++LKTVALGTSKINYLDPRITVAWCK+++VP+
Sbjct: 899 PDAKGKIKEKIAKYEEQIKKQEILKTGKDELKTVALGTSKINYLDPRITVAWCKKNKVPV 893

BLAST of CcUC10G190610 vs. ExPASy Swiss-Prot
Match: P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)

HSP 1 Score: 503.1 bits (1294), Expect = 7.0e-141
Identity = 342/894 (38.26%), Postives = 477/894 (53.36%), Query Frame = 0

Query: 129 ENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDSDDEIPL--- 188
           + +++  +D+ SDS ++ PLS  L                 NK  K   D DD IP+   
Sbjct: 23  KRISMKESDEESDSSENHPLSESL-----------------NKKSKSESDEDD-IPIRKR 82

Query: 189 --SSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPSADS 248
             SSK  M ++S     K  G             NG     K  K AV+  +      D 
Sbjct: 83  RASSKKNMSNSSSKKRAKVMG-------------NGGLKNGK--KTAVVKEEE-----DF 142

Query: 249 HSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEVDDDDDIPISQRMKKISAPVNKSSSSKQ 308
           +  ++ S K  ++S ++   +    ++K K+E D DD +P+ + +  +S    KS     
Sbjct: 143 NEIAKPSPKHKRVSKANGSKNGAKSAVK-KEESDTDDSVPL-RAVSTVSLTPYKSE---- 202

Query: 309 TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQ-- 368
            +    S++  ++    +   +  K+  S  +     D  +KWTTL HNGVIF PPY+  
Sbjct: 203 -LPSGASTTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWTTLEHNGVIFAPPYEPL 262

Query: 369 THGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGK---NHV 428
              VK++Y G PV+L PE EEVA  +A M +TD+   P F++NF+ D+ K+  +   NH 
Sbjct: 263 PKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFFRDFLKVCDECNFNHN 322

Query: 429 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 488
           I+    CDFT ++  ++ ++E+KK M  E+KKA+K++K ++EEKY W ILDG KEK    
Sbjct: 323 IKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKYKWCILDGRKEK---- 382

Query: 489 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRG 548
                                                         VGNFR+EPPGLFRG
Sbjct: 383 ----------------------------------------------VGNFRIEPPGLFRG 442

Query: 549 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 608
           RG HPK G LKRR+ P  ITINIG+G P+PE P+PG  W EV+HDNTVTWLA W++ IN 
Sbjct: 443 RGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PLPGHQWAEVKHDNTVTWLATWHENIN- 502

Query: 609 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 668
              KYVFLAA SSLKGQSD +KYEK+R LKDYI +IR  Y KD  S+    RQ   A YL
Sbjct: 503 NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDIRKGYRKDLKSELTVERQRGTAMYL 562

Query: 669 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 728
           ID  ALRAGNEK +DEADTVGCC+L+ E+V   PP  + F+FLGKDSI+Y N VEV+  V
Sbjct: 563 IDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKPPRTVVFDFLGKDSIRYYNEVEVDPQV 622

Query: 729 FKAITQF-QSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGT 788
           FK +  F +  K   D +FD+L TN LN +L  LM GL+AKVFRTYNAS T+ + L +  
Sbjct: 623 FKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSLMDGLSAKVFRTYNASYTMAEELKKMP 682

Query: 789 KDGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISEL---RDILKELKID 848
           K+  +A+KI+ Y  AN+ VAI+CNHQR+V+K+H  QM R  E+I  L   R  L+++ ++
Sbjct: 683 KNLTLADKILFYNRANRTVAILCNHQRSVTKNHDVQMERFAERIKALQYQRMRLRKMMLN 742

Query: 849 LD-RAKKGKPPL------------------------------------------------ 908
           L+ +  K KP L                                                
Sbjct: 743 LEPKLAKSKPELLAKEEGITDSWIVKHHETLYELEKEKIKKKFDRENEKLAAEDPKSVLP 802

Query: 909 -----------------KDADGKRKR----NLTPEALEKKISQTNAKIEKIERDMKTKED 939
                             DA+ K K+      + E LEK++++ N +I  +   M  K++
Sbjct: 803 ESELEVRLKAADELKKALDAELKSKKVDPGRSSMEQLEKRLNKLNERINVMRTQMIDKDE 814

BLAST of CcUC10G190610 vs. ExPASy TrEMBL
Match: A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)

HSP 1 Score: 1587.4 bits (4109), Expect = 0.0e+00
Identity = 832/940 (88.51%), Postives = 856/940 (91.06%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GR  SD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
           S+NGQSSNNQKRMT LPKTSPMRSEIGS KA KSS IQEPL K LG +LK SDSM SRPK
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 HLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDS 180
           +LVNA+VKEENV++NP+ +NSDSEDDKPLSIRLKGNSNQPNKG   T+PKNKVKK SGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK ASG +G KQ GFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 SADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEV-DDDDDIPISQRMKKISAPVNKS 300
           + DSHS SQFS KKPKLSDSS+PLSSK VSLKAKQEV DDDDDIPISQR+KK  A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK    
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK---- 480

Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRG 540
                                                         VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
           RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780

Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
           DG+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840

Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
           KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 889

Query: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
           DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of CcUC10G190610 vs. ExPASy TrEMBL
Match: A0A5A7VHI9 (DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002170 PE=3 SV=1)

HSP 1 Score: 1576.2 bits (4080), Expect = 0.0e+00
Identity = 832/958 (86.85%), Postives = 856/958 (89.35%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GR  SD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
           S+NGQSSNNQKRMT LPKTSPMRSEIGS KA KSS IQEPL K LG +LK SDSM SRPK
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 HLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDS 180
           +LVNA+VKEENV++NP+ +NSDSEDDKPLSIRLKGNSNQPNKG   T+PKNKVKK SGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK ASG +G KQ GFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 SADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEV-DDDDDIPISQRMKKISAPVNKS 300
           + DSHS SQFS KKPKLSDSS+PLSSK VSLKAKQEV DDDDDIPISQR+KK  A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK    
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK---- 480

Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRG 540
                                                         VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
           RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780

Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
           DG+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840

Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
           KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900

Query: 901 DPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAMDVDPDFRF 939
           DPRITVAWCKRHEVPIEK                  IFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAMDVDPDFRF 907

BLAST of CcUC10G190610 vs. ExPASy TrEMBL
Match: A0A0A0L2W2 (DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1)

HSP 1 Score: 1572.0 bits (4069), Expect = 0.0e+00
Identity = 824/940 (87.66%), Postives = 851/940 (90.53%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKAPMF DDDDEDNMPIVFKR+SASRQNQLDSEIKHSQ+QRL+KL GR  SD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
           S+NGQSSNNQKRMT LPKTSPMRSEIGS KAS+SS I EPL K LGT+LK S SM SRPK
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPK 120

Query: 121 HLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDS 180
            LVNA VKEENV++NP+ +NSDSEDDKPLSIRLKGN NQPNKG   TVPKNKVKK   DS
Sbjct: 121 SLVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADS 180

Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK ASG LG KQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 SADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEV-DDDDDIPISQRMKKISAPVNKS 300
           + DSHS SQF  KKPKL+DSS+PL+SK VSLKAKQEV DDDDDIPISQRMKK+SA VNKS
Sbjct: 241 NEDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK T+SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEK    
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEK---- 480

Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRG 540
                                                         VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
           RGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           FKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780

Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
           +G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 NGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840

Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
           KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 889

Query: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
           DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of CcUC10G190610 vs. ExPASy TrEMBL
Match: A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)

HSP 1 Score: 1566.2 bits (4054), Expect = 0.0e+00
Identity = 821/939 (87.43%), Postives = 850/939 (90.52%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGRP SD C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKH 120
           S+NGQSSN QKRMT LPKTSPMR EIGS KA+KSSIQEPLVK LGTTLKVSDSM SRPK 
Sbjct: 61  SANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDSD 180
           L NA+VKEEN +++PT +NSDSEDDKPLSIRLKGNSN P+KGP PTV KNK+KK S DSD
Sbjct: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPS 240
           DE+PLSSKFQMKSASG LGGKQHGF EKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLP+
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQE-VDDDDDIPISQRMKKISAPVNKSS 300
           ADSHSSSQ S KKPKLSDSSTPLS+  VSLKAKQE VDDDD IPISQRM K+SA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300

Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
            SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKIL+KGKPVDLT EQEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIY 480
           QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK     
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK----- 480

Query: 481 HFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRGR 540
                                                        VGNFRVEPPGLFRGR
Sbjct: 481 ---------------------------------------------VGNFRVEPPGLFRGR 540

Query: 541 GEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQK 600
           GEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+ WKEVRHDNTVTWLAFWNDPIN K
Sbjct: 541 GEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPK 600

Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLI 660
           EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKDPTRRQIAVATYLI
Sbjct: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLI 660

Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
           DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720

Query: 721 KAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
           KAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD
Sbjct: 721 KAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780

Query: 781 GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
           G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK
Sbjct: 781 GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840

Query: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 900
           KGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 887

Query: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
           PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of CcUC10G190610 vs. ExPASy TrEMBL
Match: A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)

HSP 1 Score: 1565.4 bits (4052), Expect = 0.0e+00
Identity = 821/939 (87.43%), Postives = 850/939 (90.52%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPASDVC 60
           MAVETFGKA MF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGRP SD C
Sbjct: 1   MAVETFGKATMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60

Query: 61  SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKH 120
           S+NGQSSN QKRMT LPKTSPMR EIGS KASKSSIQEPLVK LGTTLKVSDSM SRPK 
Sbjct: 61  STNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPTVPKNKVKKPSGDSD 180
           LVNA+VKEEN +++P  +NSDSEDDKPLSIRLKGNSN P+KGP PTV KNK+KK S DSD
Sbjct: 121 LVNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPS 240
           DE+PLSSKF+MKSASG LGGKQHGFDEKKPL  KVHQNGSTS DKLQKPAV+SSKRPLP+
Sbjct: 181 DEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQE-VDDDDDIPISQRMKKISAPVNKSS 300
           ADSHSSSQ S KKPKLSDSSTPLS+  VSLKAKQE VDDDD IPISQRM K+SA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300

Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
            SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKIL+KGKPVDLTPEQEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIY 480
           QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK     
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK----- 480

Query: 481 HFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRGR 540
                                                        VGNFRVEPPGLFRGR
Sbjct: 481 ---------------------------------------------VGNFRVEPPGLFRGR 540

Query: 541 GEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQK 600
           GEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+ WKEVRHDNTVTWLAFWNDPIN K
Sbjct: 541 GEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPK 600

Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLI 660
           EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKDPTRRQIAVATYLI
Sbjct: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLI 660

Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
           DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720

Query: 721 KAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
           KAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD
Sbjct: 721 KAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780

Query: 781 GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
           G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK
Sbjct: 781 GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840

Query: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 900
           KGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 887

Query: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
           PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of CcUC10G190610 vs. TAIR 10
Match: AT5G55300.1 (DNA topoisomerase I alpha )

HSP 1 Score: 1003.4 bits (2593), Expect = 1.2e-292
Identity = 577/990 (58.28%), Postives = 681/990 (68.79%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRPA 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    K+S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGS 120
           S V S NG         TP  KTS ++S + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKHLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGN----------SNQPNKGPGPT 180
             K      ++ E           DSEDD+PLS  L GN          S QP K     
Sbjct: 121 -QKDQSECKIEHE-----------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 VPKNKVKKPSGD-SDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDK 240
            P ++  +   D SDDE P+SS F+ K  SG  GG Q   DEKKPLV K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVLSSKRPLPSADSHSSSQFSMKKPKLSDSSTPLSSKPVS---------------- 300
           +    VL  KRPL    + S+ Q S+KK K+S S T +  K  S                
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300

Query: 301 LKAKQ--------EVDDDDDIPISQRMKKISAPVNKSSSSKQTVS-------------KV 360
           +KAKQ        + DDDDD+PIS+R K  S+  N SS+  + V               V
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360

Query: 361 VSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
           VS   +   K +KK  K SKY  S+K  PS+GDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420

Query: 421 YKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           YKGKPVDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480

Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFWKN 540
           PIY+W+  EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK              
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEK-------------- 540

Query: 541 LVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMGKL 600
                                               +GNFRVEPPGLFRGRGEHPKMGKL
Sbjct: 541 ------------------------------------IGNFRVEPPGLFRGRGEHPKMGKL 600

Query: 601 KRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFLAA 660
           K+RI P +IT+NIGKGAPIPECPI GE WKEV+HDNTVTWLAFW DPIN KEFKYVFL A
Sbjct: 601 KKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFLGA 660

Query: 661 SSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLALRAGN 720
            SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVATYLIDKLALRAGN
Sbjct: 661 GSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRAGN 720

Query: 721 EKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSG 780
           EKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ+G
Sbjct: 721 EKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAG 780

Query: 781 KSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIVT 840
           KS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KIV 
Sbjct: 781 KSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIVV 840

Query: 841 YQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKDA 900
           YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAKKGKPPL+ +
Sbjct: 841 YQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEGS 900

Query: 901 DGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCK 939
           DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWCK
Sbjct: 901 DGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWCK 913

BLAST of CcUC10G190610 vs. TAIR 10
Match: AT5G55300.2 (DNA topoisomerase I alpha )

HSP 1 Score: 998.4 bits (2580), Expect = 3.8e-291
Identity = 575/987 (58.26%), Postives = 678/987 (68.69%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRPA 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    K+S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGS 120
           S V S NG         TP  KTS ++S + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKHLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGN----------SNQPNKGPGPT 180
             K      ++ E           DSEDD+PLS  L GN          S QP K     
Sbjct: 121 -QKDQSECKIEHE-----------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 VPKNKVKKPSGD-SDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDK 240
            P ++  +   D SDDE P+SS F+ K  SG  GG Q   DEKKPLV K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVLSSKRPLPSADSHSSSQFSMKKPKLSDSSTPLSSKPVS---------------- 300
           +    VL  KRPL    + S+ Q S+KK K+S S T +  K  S                
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300

Query: 301 LKAKQ--------EVDDDDDIPISQRMKKISAPVNKSSSSKQTVS-------------KV 360
           +KAKQ        + DDDDD+PIS+R K  S+  N SS+  + V               V
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360

Query: 361 VSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
           VS   +   K +KK  K SKY  S+K  PS+GDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420

Query: 421 YKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           YKGKPVDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480

Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFWKN 540
           PIY+W+  EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK              
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEK-------------- 540

Query: 541 LVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMGKL 600
                                               +GNFRVEPPGLFRGRGEHPKMGKL
Sbjct: 541 ------------------------------------IGNFRVEPPGLFRGRGEHPKMGKL 600

Query: 601 KRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFLAA 660
           K+RI P +IT+NIGKGAPIPECPI GE WKEV+HDNTVTWLAFW DPIN KEFKYVFL A
Sbjct: 601 KKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFLGA 660

Query: 661 SSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLALRAGN 720
            SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVATYLIDKLALRAGN
Sbjct: 661 GSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRAGN 720

Query: 721 EKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSG 780
           EKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ+G
Sbjct: 721 EKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAG 780

Query: 781 KSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIVT 840
           KS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KIV 
Sbjct: 781 KSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIVV 840

Query: 841 YQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKDA 900
           YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAKKGKPPL+ +
Sbjct: 841 YQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEGS 900

Query: 901 DGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCK 936
           DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWCK
Sbjct: 901 DGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWCK 910

BLAST of CcUC10G190610 vs. TAIR 10
Match: AT5G55310.1 (DNA topoisomerase 1 beta )

HSP 1 Score: 996.1 bits (2574), Expect = 1.9e-290
Identity = 574/993 (57.80%), Postives = 692/993 (69.69%), Query Frame = 0

Query: 1   MAVETFGKAPMFD-----DDDDEDNMPIVFKRSS-----ASRQNQLDSEIKHSQSQRLNK 60
           MA E F K  + +     +D+DED++P+VFKR+S      +R + +++ +++S     + 
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIG--ST 60

Query: 61  LSGRPASDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKV 120
            S  P     +S  +S+++  R + +    P  S +      +S+++ PL        K 
Sbjct: 61  KSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSV-----QRSTLKSPLRDDRSVVAKE 120

Query: 121 SDSMGSRPKHLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGNSNQPNKGPGPT---- 180
            +  G  P             +V+ +DD  DSEDDKPLS RLK +S +  K P  +    
Sbjct: 121 RNGFGKAP-------------SVSKSDD-EDSEDDKPLSARLKLDSKEVTKQPSSSGRGS 180

Query: 181 ----VPKNKVKKPSGDS--------DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPK 240
               V K+ + +P G S        D+  P+SS  Q K++ G    K    ++K+PLV  
Sbjct: 181 TQQAVQKSNM-RPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNN 240

Query: 241 VHQNG---STSRDKLQKPAVLSSKRPLPSADSHSSSQFSMKKPKLSDSSTPL-------- 300
           + +NG    T     Q PA    KRPL      SS+Q S+K+PKLS+ + P+        
Sbjct: 241 IDRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHI 300

Query: 301 ------------SSKPVSLKAKQEV------DDDDDIPISQRMKKISAPVNKSSSSKQTV 360
                       +SKP  L+A Q        D DD +PI+ RMK  S+  NKSSS+K + 
Sbjct: 301 SATQDAKGKNLDASKP--LRANQATVKEDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSS 360

Query: 361 SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGV 420
           SK+++SS +   +K  K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGV
Sbjct: 361 SKMIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGV 420

Query: 421 KILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDC 480
           KIL++GKPVDLTPEQEEVATMFAVM++T+Y  KPKF+ENFWNDWRKLLGKNH+I++LDDC
Sbjct: 421 KILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDC 480

Query: 481 DFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAF 540
           DFTPIY+WY  EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E+           
Sbjct: 481 DFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRER----------- 540

Query: 541 WKNLVLRIFFVYFDNLPSDNVIDLLHFTVITNQQLATTEVGNFRVEPPGLFRGRGEHPKM 600
                                                  +GNFRVEPPGLFRGRGEHPKM
Sbjct: 541 ---------------------------------------IGNFRVEPPGLFRGRGEHPKM 600

Query: 601 GKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVF 660
           GKLK+RIRP DITINIGK APIPECPIPGE WKEV+HDNTVTWLAFW+DPIN KEFKYVF
Sbjct: 601 GKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYVF 660

Query: 661 LAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLALR 720
           LAASSSLKGQSDKEKYEKAR L ++I +IR AYTKDF +KD T+RQIAVATYLIDKLALR
Sbjct: 661 LAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALR 720

Query: 721 AGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQF 780
           AGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  V+KAI QF
Sbjct: 721 AGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIGQF 780

Query: 781 QSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEK 840
           Q+GKS TDDLFD+LDT+KLN HLK+LM GLTAKVFRTYNASITLD ML++ T+DGDV EK
Sbjct: 781 QAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDVPEK 840

Query: 841 IVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPL 900
           +V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDRAKKG+ PL
Sbjct: 841 VVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGRTPL 900

Query: 901 KDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVA 939
             +DGKRKRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSKINY+DPRITVA
Sbjct: 901 MGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRITVA 911

BLAST of CcUC10G190610 vs. TAIR 10
Match: AT5G55300.3 (DNA topoisomerase I alpha )

HSP 1 Score: 425.2 bits (1092), Expect = 1.3e-118
Identity = 208/271 (76.75%), Postives = 244/271 (90.04%), Query Frame = 0

Query: 668 NEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 727
           ++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ+
Sbjct: 291 DDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 350

Query: 728 GKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIV 787
           GKS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KIV
Sbjct: 351 GKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIV 410

Query: 788 TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKD 847
            YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAKKGKPPL+ 
Sbjct: 411 VYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEG 470

Query: 848 ADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWC 907
           +DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWC
Sbjct: 471 SDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWC 530

Query: 908 KRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
           KRHEVPIEKIF KSLL KFAWAMDV+P++RF
Sbjct: 531 KRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 561

HSP 2 Score: 101.7 bits (252), Expect = 3.3e-21
Identity = 108/304 (35.53%), Postives = 152/304 (50.00%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRPA 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    K+S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGS 120
           S V S NG         TP  KTS ++S + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKHLVNASVKEENVTVNPTDDNSDSEDDKPLSIRLKGN----------SNQPNKGPGPT 180
             K      ++ E           DSEDD+PLS  L GN          S QP K     
Sbjct: 121 -QKDQSECKIEHE-----------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 VPKNKVKKPSGD-SDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDK 240
            P ++  +   D SDDE P+SS F+ K  SG  GG Q   DEKKPLV K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVLSSKRPLPSADSHSSSQFSMKKPKLSDSSTPLSSKPVSLKAKQEVDDDDDIPIS 290
           +    VL  KRPL    + S+ Q S+KK K+S S T +  K  S+  K+E+DD   + +S
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSV--KKEIDDKGRVLVS 275

BLAST of CcUC10G190610 vs. TAIR 10
Match: AT4G26701.1 (DNA binding;DNA topoisomerase type Is )

HSP 1 Score: 129.0 bits (323), Expect = 2.0e-29
Identity = 60/68 (88.24%), Postives = 64/68 (94.12%), Query Frame = 0

Query: 871 KIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAM 930
           KIEK+ERDM+TKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEKIFNKSLL KFAWAM
Sbjct: 2   KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKIFNKSLLEKFAWAM 61

Query: 931 DVDPDFRF 939
           DV+P F F
Sbjct: 62  DVEPHFTF 69