CcUC10G186320 (gene) Watermelon (PI 537277) v1

BLAST of CcUC10G186320 vs. NCBI nr
Match: XP_004146208.2 (probable acyl-activating enzyme 2 [Cucumis sativus] >KAE8649992.1 hypothetical protein Csa_010505 [Cucumis sativus])

HSP 1 Score: 938.7 bits (2425), Expect = 2.2e-269
Identity = 462/540 (85.56%), Postives = 500/540 (92.59%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           M+  +HCPANF PLSP+ FL+RA+T+YGCRPSLVYG R+F+WS TYAR L+LAS L+HHF
Sbjct: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRLFSWSDTYARSLALASALLHHF 60

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           H+SP DLVVAMAPNIPELYELHFAVPMAGAIISALNTKLD+PTLSLLLQQL+PK+IF+DS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDS 120

Query: 121 QFLPVLLKSLANNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNAELD 180
           QFLP+LLKSL N+SIKFPALVLIP+DPDTP   EFLDYNKVL MRFGD  FTP+ NAELD
Sbjct: 121 QFLPILLKSLENSSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELD 180

Query: 181 PISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNGWCF 240
           PISINYTSGSTGLHKG IYSHRAAYLNSLATIFRS+ICS+TSSPVFLWTVDMFRCNGWCF
Sbjct: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSNHYTPRRLSRR 300
           IWVMAALGGCNICL TVT+DAIFT+VELH+VTLLCGP TLLKMI ESSSN+  PRRLSRR
Sbjct: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRR 300

Query: 301 VDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLEEENVQFENLITS 360
           VDLIVAGALPIKEILTKV+E+GFNISYGYGMTEAMGPAIIRPWKP+ EE+NVQFE+LITS
Sbjct: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITS 360

Query: 361 LEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGDVGVR 420
           LEIDVKDPISMESVLGDGETLGEVMLRGNTLM GYYKN+KATHEAF G+NWYRTGDVGVR
Sbjct: 361 LEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVR 420

Query: 421 HNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFVKLKN 480
           H SGRIEMKDRAKDIVVR D EGA VSTVEVE VLMSHPNVAEAAVVGERTL GFVKLKN
Sbjct: 421 HKSGRIEMKDRAKDIVVRTDGEGA-VSTVEVEGVLMSHPNVAEAAVVGERTLYGFVKLKN 480

Query: 481 GSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNANNTI 540
            S+ENGDEI+EFC MHLPEFM+P+RIVFGDLP+NSTGKVQKF +REK KAL N   NNTI
Sbjct: 481 RSKENGDEIVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKALLNNANNNTI 539

BLAST of CcUC10G186320 vs. NCBI nr
Match: XP_008448497.1 (PREDICTED: probable acyl-activating enzyme 2 [Cucumis melo])

HSP 1 Score: 923.3 bits (2385), Expect = 9.6e-265
Identity = 462/542 (85.24%), Postives = 499/542 (92.07%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           M+   HCP NFAPLSP+ FL+RA+T+YGCRPSL+YG+RVFTWSQTY RCLS+AS LVHHF
Sbjct: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           H+SP DLVVAMAPNIPELYELHFAVPMAG IISALNTKLD+PTLSLLLQQL+PK+IFLDS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120

Query: 121 QFLPVLLKSLANNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNAELD 180
           QFLP+LLKSL N SIKFPAL+LIP+DP+T    +FLDYNK+LTMR G   FTP+ NAELD
Sbjct: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD 180

Query: 181 PISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNGWCF 240
           PISINYTSGSTGLHKG IYSHRAAYLNSLATIFRS+ICS+TSSPVFLWTVDMFRCNGWCF
Sbjct: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSNHYTPRRLSRR 300
           IWVMAALGGCNICL TVT+DAIFT+VELH+VTLLCGPSTLLKMI ESSSN+  PRRLSRR
Sbjct: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR 300

Query: 301 VDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLE-EENVQF-ENLI 360
           VDLIVAGALPIKEILTKV+E+GFNISYGYGMTEAMGPAIIRPWKPS E ++NVQF ++LI
Sbjct: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI 360

Query: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGDVG 420
           TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLM GYYKNLKATHEAF G+NWYRTGDVG
Sbjct: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 420

Query: 421 VRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFVKL 480
           VRH SGRIEMKDRAKDIVVR D EGA VSTVEVE VLMSHPNVAE AVVGERTL GFVKL
Sbjct: 421 VRHKSGRIEMKDRAKDIVVRTDGEGA-VSTVEVEGVLMSHPNVAETAVVGERTLYGFVKL 480

Query: 481 KNGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNANN 540
           KNGS+EN DEI+EFC MHLPEFMVP+ IVFGDLP+NSTGKVQKFV+REKAKAL N N NN
Sbjct: 481 KNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGN-NN 540

BLAST of CcUC10G186320 vs. NCBI nr
Match: XP_038903286.1 (probable acyl-activating enzyme 2 [Benincasa hispida])

HSP 1 Score: 917.5 bits (2370), Expect = 5.2e-263
Identity = 460/541 (85.03%), Postives = 494/541 (91.31%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           M+  FHCPANF PLSP+ FL+RAATVYG RPS+VYGARV+TWS+TY RCL+LAS LVHHF
Sbjct: 1   MEGFFHCPANFTPLSPVGFLKRAATVYGGRPSVVYGARVYTWSETYGRCLTLASALVHHF 60

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLD+  LSLLLQQL+PKIIFLDS
Sbjct: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSSALSLLLQQLNPKIIFLDS 120

Query: 121 QFLPVLLKSLANNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFG-DGGFTPKLNAEL 180
           QFLP+LLKSL NNSIKFPALVLI T+P+T   LE+ DYN+VL MRF  D  FTP+ NAEL
Sbjct: 121 QFLPILLKSLENNSIKFPALVLISTNPNT-SRLEYFDYNRVLAMRFSDDNDFTPRSNAEL 180

Query: 181 DPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNGWC 240
           DPISINYTSGSTGLHKG IYSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNGWC
Sbjct: 181 DPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSGICKSTSSPVFLWTVDMFRCNGWC 240

Query: 241 FIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSNHYTPRRLSR 300
           FIW MAALGGCNICL TVT++AIFT+VELHKVTLLCGPSTLLKMISES SNHY  RRLSR
Sbjct: 241 FIWAMAALGGCNICLRTVTAEAIFTNVELHKVTLLCGPSTLLKMISESLSNHYMSRRLSR 300

Query: 301 RVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLEEENVQFENLIT 360
           RVDLIVAGALPIKEILTKV+E+GFNISYGYGMTEAMGP IIRPWKP+ EEE VQFE+LIT
Sbjct: 301 RVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPVIIRPWKPNFEEETVQFEDLIT 360

Query: 361 SLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGDVGV 420
           SLEIDVKDPISMESVLGDGETLGEVMLRGNTLM GYYKNLKATHEAFSGDNWYRTGD+G+
Sbjct: 361 SLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFSGDNWYRTGDIGL 420

Query: 421 RHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFVKLK 480
           RH SGRIEMKDRAKDIVVRAD EG VVSTVEVE VLMSHP+VAEAAVV ER LCGFVK+K
Sbjct: 421 RHKSGRIEMKDRAKDIVVRADGEG-VVSTVEVEGVLMSHPDVAEAAVVEERMLCGFVKMK 480

Query: 481 NGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNANNT 540
           +  +ENGDEI+EFC M+LPEFMVP++IVFGDLP+NS GKVQKFVVREKAK    LNANNT
Sbjct: 481 DERKENGDEIVEFCRMYLPEFMVPKKIVFGDLPMNSIGKVQKFVVREKAK---TLNANNT 536

BLAST of CcUC10G186320 vs. NCBI nr
Match: KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 854.4 bits (2206), Expect = 5.5e-244
Identity = 438/544 (80.51%), Postives = 476/544 (87.50%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           MD     PANF PLSP+ F +RAA VY  RPS++YG+RVFTWS+TY R L LAS LVHHF
Sbjct: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           H+SPGD+V AMAPN+PELYELHFAVPMAGAIIS LNTKLD PTLSLLLQQLHPK+IFLDS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120

Query: 121 QFLPVLLKSLA---NNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNA 180
            FLP +LKSL+    NSI+FPALVLIPT PDTPPS EFLDYN+VL MRF D  FTP+ NA
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPTHPDTPPS-EFLDYNRVLGMRFDD--FTPRTNA 180

Query: 181 ELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKG +YSHRAAYLNSLATIFRS IC NTSSPVFLWTVDMFRCNG
Sbjct: 181 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 240

Query: 241 WCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSS-NHYTPRR 300
           WCFIW MAALGGCNICL  VT+DAIFT++ELHKVTLLCGPSTLLKMI ESSS N++ P  
Sbjct: 241 WCFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKP-W 300

Query: 301 LSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLEEENVQFEN 360
            SRRVDLIVAGALPI EILTKV E+GFNISYGYGMTEAMGPA+IRPWKP+ +EE VQFE+
Sbjct: 301 FSRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEEIVQFED 360

Query: 361 LITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGD 420
           L TSLEIDVKDP+SMESVLGDGETLGEVMLRGNTLM GYYKNLKAT EAFSGD WYRTGD
Sbjct: 361 LFTSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD-WYRTGD 420

Query: 421 VGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFV 480
           VGVRH SGRIEMKDRAKDIVVRA+ E A VSTV+VEAVLMSHP+VAEAAVVGER LCGFV
Sbjct: 421 VGVRHKSGRIEMKDRAKDIVVRANGE-AAVSTVQVEAVLMSHPSVAEAAVVGER-LCGFV 480

Query: 481 KLKNGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNA 540
           KLKNGSR + +EI+EFC  HLPEFM+PER+VFGDLP+NS GKVQKF+VREKAK    LN 
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAK---ELNT 534

BLAST of CcUC10G186320 vs. NCBI nr
Match: KAG7015844.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 853.6 bits (2204), Expect = 9.3e-244
Identity = 437/544 (80.33%), Postives = 475/544 (87.32%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           MD     PANF PLSP+ F +RAA VY  RPS++YG+RVFTWS+TY R L LAS LVHHF
Sbjct: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           H+SPGD+V AMAPN+PELYELHFAVPMAGAIIS LNTKLD PTLSLLLQQLHPK+IFLDS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120

Query: 121 QFLPVLLKSLA---NNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNA 180
            FLP +LKSL+    NSI+FPALVLIP  PDTPPS EFLDYN+VL MRF D  FTP+ NA
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPS-EFLDYNRVLGMRFDD--FTPRTNA 180

Query: 181 ELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKG +YSHRAAYLNSLATIFRS IC NTSSPVFLWTVDMFRCNG
Sbjct: 181 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 240

Query: 241 WCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSS-NHYTPRR 300
           WCFIW MAALGGCNICL  VT+DAIFT++ELHKVTLLCGPSTLLKMI ESSS N++ P  
Sbjct: 241 WCFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKP-W 300

Query: 301 LSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLEEENVQFEN 360
            SRRVDLIVAGALPI EILTKV E+GFNISYGYGMTEAMGPA+IRPWKP+ +EE VQFE+
Sbjct: 301 FSRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFED 360

Query: 361 LITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGD 420
           L TSLEIDVKDP+SMESVLGDGETLGEVMLRGNTLM GYYKNLKAT EAFSGD WYRTGD
Sbjct: 361 LFTSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD-WYRTGD 420

Query: 421 VGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFV 480
           VGVRH SGRIEMKDRAKDIVVRA+ E A VSTV+VEAVLMSHP+VAEAAVVGER LCGFV
Sbjct: 421 VGVRHKSGRIEMKDRAKDIVVRANGE-ATVSTVQVEAVLMSHPSVAEAAVVGER-LCGFV 480

Query: 481 KLKNGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNA 540
           KLKNGSR + +EI+EFC  HLPEFM+PER+VFGDLP+NS GKVQKF+VREKAK    LN 
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAK---ELNT 534

BLAST of CcUC10G186320 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 5.5e-130
Identity = 261/566 (46.11%), Postives = 356/566 (62.90%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           ++ +   PANF+PLSPI FLER+A VY  R SLV+G+   TW QTY RCL LAS L  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
            +S GD+V A+APN+P ++ELHFAVPMAG I+  LNT+LD  TLS+LL     KI+F+D 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QFLPVL-----LKSLANNSIKFPALVLIPTDPDTPPSLE----------FLDYNKVLTMR 180
           Q L +      L + ++ + K   LVLI    D   S E            DY     ++
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLK 227

Query: 181 FGDGGF-TPKLNAELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSP 240
            GD  F   K   E DPISINYTSG+T   KG +YSHR AYLNSLAT+F  ++   +  P
Sbjct: 228 SGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM---SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICL  V+   IF ++ +HKVT + G  T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 SESSSNHYTPRRLSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWK 360
              +   + P  L  RV+++  G+ P+ +IL K++E+GFN+S+ YG+TE  GP     WK
Sbjct: 348 VNYTVTEHKP--LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWK 407

Query: 361 PSLEEENVQFENLITSLE---------IDVKDPISMESVLGDGETLGEVMLRGNTLMCGY 420
           P  +  +++    + + +         +DVKDP++ME+V  DG T+GEVM RGNT+M GY
Sbjct: 408 PEWDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGY 467

Query: 421 YKNLKATHEAFSGDNWYRTGDVGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVL 480
           +K+++AT +AF GD W+ +GD+ V++  G IE+KDR KD+++     G  +S+VEVE VL
Sbjct: 468 FKDIEATRKAFEGD-WFHSGDLAVKYPDGYIEIKDRLKDVIISG---GENISSVEVERVL 527

Query: 481 MSHPNVAEAAVVGE------RTLCGFVKLKNG-SRENGDEIIEFCGMHLPEFMVPERIVF 535
            SH  V EAAVV        +T CGFVKLK G      +EII FC  HLP +M P+ IVF
Sbjct: 528 CSHQAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVF 587

BLAST of CcUC10G186320 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 2.3e-128
Identity = 262/557 (47.04%), Postives = 349/557 (62.66%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           M+ +  C AN  PLSPI FLER++  Y    SLVYG+  +TW+QT+ RCL LAS L  HF
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
            +SPGD+V   + NIPE+YELHFAVPMAG I+  LN + D+  +S LL     K+IF++ 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLP---VLLKSLANNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNA 180
           Q L      L  LA   IK P LVL+ TD ++  S  +  YN +L     D       N 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLL-TDSESFTSSSYDHYNHLLANGSDDFEIRRPKN- 208

Query: 181 ELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNG 240
           E DPISINYTSG+T   K  +YSHR AYLNS+AT+    + + +   V+LW+V MF CNG
Sbjct: 209 ECDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTRS---VYLWSVPMFHCNG 268

Query: 241 WCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISES-SSNHYTPRR 300
           WCF W  AA G  NIC+  V+  AIF ++ LHKVT      T+L MI  S   N +TP  
Sbjct: 269 WCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTP-- 328

Query: 301 LSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPA---IIRP-WKPSLEEENV 360
           L  +V+++  G+ P  +++ +++EMGF +++ YG+TE  GPA   + +P W     EE  
Sbjct: 329 LPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERY 388

Query: 361 QFE-----NLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFS 420
             +     N +   E+DV+DP+SMESV  DG T+GEVM RGNT+M GY+K+LKAT EAF 
Sbjct: 389 ALKARQGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFE 448

Query: 421 GDNWYRTGDVGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVV 480
           G  W+RTGD+GV+H  G I++KDR KD+V+     G  VSTVEVE VL SH  V EAAVV
Sbjct: 449 G-GWFRTGDLGVKHEDGYIQLKDRKKDVVISG---GENVSTVEVETVLYSHEAVLEAAVV 508

Query: 481 GE------RTLCGFVKLKNGSRENG---DEIIEFCGMHLPEFMVPERIVFGDLPINSTGK 536
                    T C FV LK G  +NG   D+II+FC   LP +M P+ +VF +LP  STGK
Sbjct: 509 ARPDKLWGETPCAFVTLKEGF-DNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGK 568

BLAST of CcUC10G186320 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 6.3e-126
Identity = 254/556 (45.68%), Postives = 346/556 (62.23%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           M+ +  C AN  PLSPI FLER++  Y    SLVYG+  +TW+QT+ RCL LAS L  H 
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
            +SPGD+V   + N+PE+YELHFAVPMAG I+  LN + D+  +S LL     K+IF++ 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLP---VLLKSLANNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNA 180
           Q L      L  LA   IK P LVL+ TD ++  S  +  YN +L     D       N 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLL-TDSESFTSSSYDHYNHLLANGSDDFEIRRPKN- 208

Query: 181 ELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNG 240
           E DPISINYTSG+T   K  +YSHR AYLNS+AT+    +    ++ V+LW+V MF CNG
Sbjct: 209 EWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVL---LHGMGTTSVYLWSVPMFHCNG 268

Query: 241 WCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISES-SSNHYTPRR 300
           WCF W  AA G  NIC+  V+  AIF ++ LHKVT      T+L MI  S   N +TP  
Sbjct: 269 WCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTP-- 328

Query: 301 LSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPA---IIRP-WKPSLEEENV 360
           L  +V+++  G+ P  +++ +++EMGF +++ YG+TE  GPA   + +P W     EE  
Sbjct: 329 LPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERY 388

Query: 361 QFE-----NLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFS 420
             +     N +   E+DV+DP++MESV  DG T+GEVM RGNT+M GY+K+LKAT EAF 
Sbjct: 389 ALKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFE 448

Query: 421 GDNWYRTGDVGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVV 480
           G  W+R+GD+GV+H  G I++KDR KD+V+     G  +STVEVE VL SH  V EAAVV
Sbjct: 449 G-GWFRSGDLGVKHEDGYIQLKDRKKDVVISG---GENISTVEVETVLYSHEAVLEAAVV 508

Query: 481 GE------RTLCGFVKLKNG--SRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKV 536
                    T C FV LK G  +  + D+II+FC   LP +M P+ +VF +LP  STGK+
Sbjct: 509 ARPDKLWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKI 568

BLAST of CcUC10G186320 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 6.9e-125
Identity = 252/560 (45.00%), Postives = 346/560 (61.79%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           M+     PAN+ PL+PI FL+R+A VY  R S+VYG+  +TW QT  RC+ +AS L    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
            +S GD+V  +APN+P + ELHF VPMAGA++  LN + D+  +++LL+    K+IF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QFLPV---LLKSLANNSIKFPALVLIPTDPDTPP------SLEFLDYNKVLTMRFGDGGF 180
           QFL +     + L+N   K P LVLIP +P T        S E ++Y  V+ M  G   F
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAM--GKSDF 182

Query: 181 -TPKLNAELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTV 240
              +   E D IS+NYTSG+T   KG +YSHR AYLNSLA +  + +    SSP +LWT 
Sbjct: 183 EVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEM---HSSPTYLWTN 242

Query: 241 DMFRCNGWCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSN 300
            MF CNGWC +W + A+GG NICL  VT+ AIF ++  HKVT + G  T+L MI  +  +
Sbjct: 243 PMFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPES 302

Query: 301 HYTPRRLSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLE-- 360
              P  L  +V  I   A P   ++ K++E+GF++ + YG+TE  GP  I  WKP  +  
Sbjct: 303 EQKP--LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSL 362

Query: 361 --EENVQFE-----NLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKA 420
             EE  + +     N +   EI VKDP++M ++  DG T+GEV+ RGNT+M GY KN +A
Sbjct: 363 PREEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEA 422

Query: 421 THEAFSGDNWYRTGDVGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNV 480
           T EAF G  W+ +GD+GV+H  G IE+KDR+KDI++     G  +S++EVE+ L +HP V
Sbjct: 423 TKEAFKG-GWFWSGDLGVKHPDGYIELKDRSKDIIISG---GENISSIEVESTLFTHPCV 482

Query: 481 AEAAVVGE------RTLCGFVKLKNGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINS 536
            EAAVV         T C FVKLK+GS+ + +E+I +C   LP +M P  IVF DLP  S
Sbjct: 483 LEAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTS 542

BLAST of CcUC10G186320 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 6.5e-107
Identity = 221/562 (39.32%), Postives = 338/562 (60.14%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           MD +  C AN  PL+PI FL+RA+  Y  R S++YG   FTW QTY RC  LA+ L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           +++  D+V  +APN+P +YE+HF+VPM GA+++ +NT+LD  T++++L+   PKI+F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QFLPVLLKSL----ANNSIKFPALVLI-PTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKL 180
           +F P++ + L       S   P ++LI   D  T P  + LDY  ++      G  TP  
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIR----KGEPTPSS 180

Query: 181 NA-------ELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLW 240
           +A       E DPIS+NYTSG+T   KG + SH+ AYL++L++I    +      PV+LW
Sbjct: 181 SASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM---GIFPVYLW 240

Query: 241 TVDMFRCNGWCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESS 300
           T+ MF CNGW   W +AA GG N+C+  VT+  I+ ++ELH VT +    T+ + + E S
Sbjct: 241 TLPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGS 300

Query: 301 SNHYTPRRLSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWK---- 360
               +P+  S  V ++  G+ P   ++ KV+++GF++ +GYG+TEA GP +   W+    
Sbjct: 301 RTDQSPK--SSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWN 360

Query: 361 -----PSLEEENVQFENLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNL 420
                  +E +  Q    +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN 
Sbjct: 361 KLPEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNP 420

Query: 421 KATHEAFSGDNWYRTGDVGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHP 480
           KAT EAF    W  TGD+GV H  G +E+KDR+KDI++     G  +S++EVE VL  + 
Sbjct: 421 KATSEAFK-HGWLNTGDIGVIHPDGYVEIKDRSKDIIISG---GENISSIEVEKVLYMYQ 480

Query: 481 NVAEAAVVG------ERTLCGFVKLKNGSR---ENGDEIIEFCGMHLPEFMVPERIV-FG 532
            V EAAVV         T C FV LK G      +  ++I++C  ++P FM P+++V F 
Sbjct: 481 EVLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQ 540

BLAST of CcUC10G186320 vs. ExPASy TrEMBL
Match: A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)

HSP 1 Score: 923.3 bits (2385), Expect = 4.6e-265
Identity = 462/542 (85.24%), Postives = 499/542 (92.07%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           M+   HCP NFAPLSP+ FL+RA+T+YGCRPSL+YG+RVFTWSQTY RCLS+AS LVHHF
Sbjct: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           H+SP DLVVAMAPNIPELYELHFAVPMAG IISALNTKLD+PTLSLLLQQL+PK+IFLDS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120

Query: 121 QFLPVLLKSLANNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNAELD 180
           QFLP+LLKSL N SIKFPAL+LIP+DP+T    +FLDYNK+LTMR G   FTP+ NAELD
Sbjct: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD 180

Query: 181 PISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNGWCF 240
           PISINYTSGSTGLHKG IYSHRAAYLNSLATIFRS+ICS+TSSPVFLWTVDMFRCNGWCF
Sbjct: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSNHYTPRRLSRR 300
           IWVMAALGGCNICL TVT+DAIFT+VELH+VTLLCGPSTLLKMI ESSSN+  PRRLSRR
Sbjct: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR 300

Query: 301 VDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLE-EENVQF-ENLI 360
           VDLIVAGALPIKEILTKV+E+GFNISYGYGMTEAMGPAIIRPWKPS E ++NVQF ++LI
Sbjct: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI 360

Query: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGDVG 420
           TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLM GYYKNLKATHEAF G+NWYRTGDVG
Sbjct: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 420

Query: 421 VRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFVKL 480
           VRH SGRIEMKDRAKDIVVR D EGA VSTVEVE VLMSHPNVAE AVVGERTL GFVKL
Sbjct: 421 VRHKSGRIEMKDRAKDIVVRTDGEGA-VSTVEVEGVLMSHPNVAETAVVGERTLYGFVKL 480

Query: 481 KNGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNANN 540
           KNGS+EN DEI+EFC MHLPEFMVP+ IVFGDLP+NSTGKVQKFV+REKAKAL N N NN
Sbjct: 481 KNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGN-NN 540

BLAST of CcUC10G186320 vs. ExPASy TrEMBL
Match: A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.5e-244
Identity = 437/544 (80.33%), Postives = 478/544 (87.87%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           MD     PANF PLSP+ FL+++A VY  RPS++YG+RVFTWS+TY R L LAS LVHHF
Sbjct: 49  MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           HVSPGD+V AMAPN+PELYELHFAVPMAGAIIS LNTKLD PTLSLLLQQLHPK+IFLDS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168

Query: 121 QFLPVLLKSLA---NNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNA 180
            FLP +L+SL+    NSI+FPALVLIP  PDTPPS EFLDYN+VL MRF D  FTP+ NA
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPS-EFLDYNRVLGMRFDD--FTPRTNA 228

Query: 181 ELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKG +YSHRAAYLNSLATIFRS IC NTSSPVFLWTVDMFRCNG
Sbjct: 229 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 288

Query: 241 WCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSS-NHYTPRR 300
           WCFIW MAALGGCNICL  VT+DAIFT++ELHKVTLLCGPSTLLKMI ESSS N++ P  
Sbjct: 289 WCFIWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKP-W 348

Query: 301 LSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLEEENVQFEN 360
           LSRRVDLIVAGALPI EILTKV E+GFNISYGYGMTEAMGPA+IRPWKP+ +EE VQFE+
Sbjct: 349 LSRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFED 408

Query: 361 LITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGD 420
           LITSLEIDVKDP+SMESVLGDGETLGEVMLRGNTLM GYYKNLKAT EAFSGD WYRTGD
Sbjct: 409 LITSLEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD-WYRTGD 468

Query: 421 VGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFV 480
           VGVRH SGRIEMKDRAKDIVVRA+ E A VSTV+VEAVLMSHP+VAEAAVVGER LCGFV
Sbjct: 469 VGVRHKSGRIEMKDRAKDIVVRANGEVA-VSTVQVEAVLMSHPSVAEAAVVGER-LCGFV 528

Query: 481 KLKNGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNA 540
           KLKNGSR + +EI++FC  HLPEFM+PER+VFGDLP+NS GKVQKF+ REKAKA   LN 
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKA---LNT 582

BLAST of CcUC10G186320 vs. ExPASy TrEMBL
Match: A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 2.2e-243
Identity = 432/543 (79.56%), Postives = 470/543 (86.56%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           MD  F  PANF PLSP+ F +RAA VYG RPS++YG+RVFTWS+TY RCL LAS LVHHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           H+SPGD+V AMAPN+PELYELHFAVPMAGAIIS LNTKLD PTLSLLLQQLHPK+IFLDS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 QFLPVLLKSLA---NNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNA 180
            FLP LL+SL+    NSI+FPALVLIP  PDTPPS EFLDYN+VL MRF +  FTP+ N+
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPS-EFLDYNRVLEMRFDE--FTPRTNS 221

Query: 181 ELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKG +YSHRAAYLNSLATIFRS IC NTSSPVFLWTVDMFRCNG
Sbjct: 222 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 281

Query: 241 WCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSNHYTPRRL 300
           WCFIW MAALGGCNICL  VT DAIFT++ELHKVTLLCGPSTLLKMI ESSS +      
Sbjct: 282 WCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGF 341

Query: 301 SRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLEEENVQFENL 360
            RRVDLIVAGALPI EILTKV E+GFNISYGYGMTEAMGPA+IRPWKP+ +EE VQFE+L
Sbjct: 342 LRRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDL 401

Query: 361 ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGDV 420
           I SLEIDVKDP SMESVLGDGETLGEVMLRGN+LM GYYKNLKAT EAFSGD WYRTGDV
Sbjct: 402 IASLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGD-WYRTGDV 461

Query: 421 GVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFVK 480
           GV+H SGRIEMKDRAKDIVVR + EGA VSTV+VEAVLMSHP VAEAAV+GER LCG VK
Sbjct: 462 GVKHKSGRIEMKDRAKDIVVRENGEGA-VSTVQVEAVLMSHPGVAEAAVIGER-LCGLVK 521

Query: 481 LKNGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNAN 540
           LKNGSR + +EI+EFC  HLPEFM+PER+VFGDLP+NS GKVQKF+ REKAKA   LN N
Sbjct: 522 LKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKA---LNTN 575

BLAST of CcUC10G186320 vs. ExPASy TrEMBL
Match: A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 1.3e-235
Identity = 412/470 (87.66%), Postives = 439/470 (93.40%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDSQFLPVLLKSL 130
           MAPNIPELYELHFAVPMAGAIISALNTKLD+PTLSLLLQQL+PK+IF+DSQFLP+LLKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60

Query: 131 ANNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNAELDPISINYTSGS 190
            N+SIKFPALVLIP+DPDTP   EFLDYNKVL MRFGD  FTP+ NAELDPISINYTSGS
Sbjct: 61  ENSSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTSGS 120

Query: 191 TGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 250
           TGLHKG IYSHRAAYLNSLATIFRS+ICS+TSSPVFLWTVDMFRCNGWCFIWVMAALGGC
Sbjct: 121 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 180

Query: 251 NICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSNHYTPRRLSRRVDLIVAGALP 310
           NICL TVT+DAIFT+VELH+VTLLCGP TLLKMI ESSSN+  PRRLSRRVDLIVAGALP
Sbjct: 181 NICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 240

Query: 311 IKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLEEENVQFENLITSLEIDVKDPIS 370
           IKEILTKV+E+GFNISYGYGMTEAMGPAIIRPWKP+ EE+NVQFE+LITSLEIDVKDPIS
Sbjct: 241 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKDPIS 300

Query: 371 MESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGDVGVRHNSGRIEMKD 430
           MESVLGDGETLGEVMLRGNTLM GYYKN+KATHEAF G+NWYRTGDVGVRH SGRIEMKD
Sbjct: 301 MESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEMKD 360

Query: 431 RAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFVKLKNGSRENGDEII 490
           RAKDIVVR D EGA VSTVEVE VLMSHPNVAEAAVVGERTL GFVKLKN S+ENGDEI+
Sbjct: 361 RAKDIVVRTDGEGA-VSTVEVEGVLMSHPNVAEAAVVGERTLYGFVKLKNRSKENGDEIV 420

Query: 491 EFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNANNTI 541
           EFC MHLPEFM+P+RIVFGDLP+NSTGKVQKF +REK KAL N   NNTI
Sbjct: 421 EFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKALLNNANNNTI 469

BLAST of CcUC10G186320 vs. ExPASy TrEMBL
Match: A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 3.1e-229
Identity = 409/472 (86.65%), Postives = 437/472 (92.58%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDSQFLPVLLKSL 130
           MAPNIPELYELHFAVPMAG IISALNTKLD+PTLSLLLQQL+PK+IFLDSQFLP+LLKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 131 ANNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKLNAELDPISINYTSGS 190
            N SIKFPAL+LIP+DP+T    +FLDYNK+LTMR G   FTP+ NAELDPISINYTSGS
Sbjct: 61  ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS 120

Query: 191 TGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 250
           TGLHKG IYSHRAAYLNSLATIFRS+ICS+TSSPVFLWTVDMFRCNGWCFIWVMAALGGC
Sbjct: 121 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 180

Query: 251 NICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSNHYTPRRLSRRVDLIVAGALP 310
           NICL TVT+DAIFT+VELH+VTLLCGPSTLLKMI ESSSN+  PRRLSRRVDLIVAGALP
Sbjct: 181 NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 240

Query: 311 IKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLE-EENVQF-ENLITSLEIDVKDP 370
           IKEILTKV+E+GFNISYGYGMTEAMGPAIIRPWKPS E ++NVQF ++LITSLEIDVKDP
Sbjct: 241 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP 300

Query: 371 ISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGDVGVRHNSGRIEM 430
           ISMESVLGDGETLGEVMLRGNTLM GYYKNLKATHEAF G+NWYRTGDVGVRH SGRIEM
Sbjct: 301 ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM 360

Query: 431 KDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGERTLCGFVKLKNGSRENGDE 490
           KDRAKDIVVR D EGA VSTVEVE VLMSHPNVAE AVVGERTL GFVKLKNGS+EN DE
Sbjct: 361 KDRAKDIVVRTDGEGA-VSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDE 420

Query: 491 IIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALNNLNANNTI 541
           I+EFC MHLPEFMVP+ IVFGDLP+NSTGKVQKFV+REKAKAL N N NNTI
Sbjct: 421 IVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGN-NNTI 470

BLAST of CcUC10G186320 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 466.1 bits (1198), Expect = 3.9e-131
Identity = 261/566 (46.11%), Postives = 356/566 (62.90%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           ++ +   PANF+PLSPI FLER+A VY  R SLV+G+   TW QTY RCL LAS L  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
            +S GD+V A+APN+P ++ELHFAVPMAG I+  LNT+LD  TLS+LL     KI+F+D 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QFLPVL-----LKSLANNSIKFPALVLIPTDPDTPPSLE----------FLDYNKVLTMR 180
           Q L +      L + ++ + K   LVLI    D   S E            DY     ++
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLK 227

Query: 181 FGDGGF-TPKLNAELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSP 240
            GD  F   K   E DPISINYTSG+T   KG +YSHR AYLNSLAT+F  ++   +  P
Sbjct: 228 SGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM---SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICL  V+   IF ++ +HKVT + G  T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 SESSSNHYTPRRLSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWK 360
              +   + P  L  RV+++  G+ P+ +IL K++E+GFN+S+ YG+TE  GP     WK
Sbjct: 348 VNYTVTEHKP--LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWK 407

Query: 361 PSLEEENVQFENLITSLE---------IDVKDPISMESVLGDGETLGEVMLRGNTLMCGY 420
           P  +  +++    + + +         +DVKDP++ME+V  DG T+GEVM RGNT+M GY
Sbjct: 408 PEWDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGY 467

Query: 421 YKNLKATHEAFSGDNWYRTGDVGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVL 480
           +K+++AT +AF GD W+ +GD+ V++  G IE+KDR KD+++     G  +S+VEVE VL
Sbjct: 468 FKDIEATRKAFEGD-WFHSGDLAVKYPDGYIEIKDRLKDVIISG---GENISSVEVERVL 527

Query: 481 MSHPNVAEAAVVGE------RTLCGFVKLKNG-SRENGDEIIEFCGMHLPEFMVPERIVF 535
            SH  V EAAVV        +T CGFVKLK G      +EII FC  HLP +M P+ IVF
Sbjct: 528 CSHQAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVF 587

BLAST of CcUC10G186320 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 449.1 bits (1154), Expect = 4.9e-126
Identity = 252/560 (45.00%), Postives = 346/560 (61.79%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           M+     PAN+ PL+PI FL+R+A VY  R S+VYG+  +TW QT  RC+ +AS L    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
            +S GD+V  +APN+P + ELHF VPMAGA++  LN + D+  +++LL+    K+IF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QFLPV---LLKSLANNSIKFPALVLIPTDPDTPP------SLEFLDYNKVLTMRFGDGGF 180
           QFL +     + L+N   K P LVLIP +P T        S E ++Y  V+ M  G   F
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAM--GKSDF 182

Query: 181 -TPKLNAELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTV 240
              +   E D IS+NYTSG+T   KG +YSHR AYLNSLA +  + +    SSP +LWT 
Sbjct: 183 EVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEM---HSSPTYLWTN 242

Query: 241 DMFRCNGWCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSN 300
            MF CNGWC +W + A+GG NICL  VT+ AIF ++  HKVT + G  T+L MI  +  +
Sbjct: 243 PMFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPES 302

Query: 301 HYTPRRLSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLE-- 360
              P  L  +V  I   A P   ++ K++E+GF++ + YG+TE  GP  I  WKP  +  
Sbjct: 303 EQKP--LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSL 362

Query: 361 --EENVQFE-----NLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKA 420
             EE  + +     N +   EI VKDP++M ++  DG T+GEV+ RGNT+M GY KN +A
Sbjct: 363 PREEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEA 422

Query: 421 THEAFSGDNWYRTGDVGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNV 480
           T EAF G  W+ +GD+GV+H  G IE+KDR+KDI++     G  +S++EVE+ L +HP V
Sbjct: 423 TKEAFKG-GWFWSGDLGVKHPDGYIELKDRSKDIIISG---GENISSIEVESTLFTHPCV 482

Query: 481 AEAAVVGE------RTLCGFVKLKNGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINS 536
            EAAVV         T C FVKLK+GS+ + +E+I +C   LP +M P  IVF DLP  S
Sbjct: 483 LEAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTS 542

BLAST of CcUC10G186320 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 389.4 bits (999), Expect = 4.6e-108
Identity = 221/562 (39.32%), Postives = 338/562 (60.14%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           MD +  C AN  PL+PI FL+RA+  Y  R S++YG   FTW QTY RC  LA+ L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           +++  D+V  +APN+P +YE+HF+VPM GA+++ +NT+LD  T++++L+   PKI+F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QFLPVLLKSL----ANNSIKFPALVLI-PTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKL 180
           +F P++ + L       S   P ++LI   D  T P  + LDY  ++      G  TP  
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIR----KGEPTPSS 180

Query: 181 NA-------ELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLW 240
           +A       E DPIS+NYTSG+T   KG + SH+ AYL++L++I    +      PV+LW
Sbjct: 181 SASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM---GIFPVYLW 240

Query: 241 TVDMFRCNGWCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESS 300
           T+ MF CNGW   W +AA GG N+C+  VT+  I+ ++ELH VT +    T+ + + E S
Sbjct: 241 TLPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGS 300

Query: 301 SNHYTPRRLSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWK---- 360
               +P+  S  V ++  G+ P   ++ KV+++GF++ +GYG+TEA GP +   W+    
Sbjct: 301 RTDQSPK--SSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWN 360

Query: 361 -----PSLEEENVQFENLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNL 420
                  +E +  Q    +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN 
Sbjct: 361 KLPEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNP 420

Query: 421 KATHEAFSGDNWYRTGDVGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHP 480
           KAT EAF    W  TGD+GV H  G +E+KDR+KDI++     G  +S++EVE VL  + 
Sbjct: 421 KATSEAFK-HGWLNTGDIGVIHPDGYVEIKDRSKDIIISG---GENISSIEVEKVLYMYQ 480

Query: 481 NVAEAAVVG------ERTLCGFVKLKNGSR---ENGDEIIEFCGMHLPEFMVPERIV-FG 532
            V EAAVV         T C FV LK G      +  ++I++C  ++P FM P+++V F 
Sbjct: 481 EVLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQ 540

BLAST of CcUC10G186320 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 377.9 bits (969), Expect = 1.4e-104
Identity = 216/483 (44.72%), Postives = 296/483 (61.28%), Query Frame = 0

Query: 78  LYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDSQFLPV---LLKSLANNS 137
           + ELHF VPMAGA++  LN + D+  +++LL+    K+IF D QFL +     + L+N  
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 138 IKFPALVLIPTDPDTPP------SLEFLDYNKVLTMRFGDGGF-TPKLNAELDPISINYT 197
            K P LVLIP +P T        S E ++Y  V+ M  G   F   +   E D IS+NYT
Sbjct: 61  DKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAM--GKSDFEVIRPTDECDAISVNYT 120

Query: 198 SGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLWTVDMFRCNGWCFIWVMAAL 257
           SG+T   KG +YSHR AYLNSLA +  + +    SSP +LWT  MF CNGWC +W + A+
Sbjct: 121 SGTTSSPKGVVYSHRGAYLNSLAAVLLNEM---HSSPTYLWTNPMFHCNGWCLLWGVTAI 180

Query: 258 GGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESSSNHYTPRRLSRRVDLIVAG 317
           GG NICL  VT+ AIF ++  HKVT + G  T+L MI  +  +   P  L  +V  I   
Sbjct: 181 GGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP--LPGKVSFITGA 240

Query: 318 ALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLE----EENVQFE-----NLI 377
           A P   ++ K++E+GF++ + YG+TE  GP  I  WKP  +    EE  + +     N +
Sbjct: 241 APPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHL 300

Query: 378 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNLKATHEAFSGDNWYRTGDVG 437
              EI VKDP++M ++  DG T+GEV+ RGNT+M GY KN +AT EAF G  W+ +GD+G
Sbjct: 301 GLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKG-GWFWSGDLG 360

Query: 438 VRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHPNVAEAAVVGE------RTL 497
           V+H  G IE+KDR+KDI++     G  +S++EVE+ L +HP V EAAVV         T 
Sbjct: 361 VKHPDGYIELKDRSKDIIISG---GENISSIEVESTLFTHPCVLEAAVVARPDEYWGETA 420

Query: 498 CGFVKLKNGSRENGDEIIEFCGMHLPEFMVPERIVFGDLPINSTGKVQKFVVREKAKALN 536
           C FVKLK+GS+ + +E+I +C   LP +M P  IVF DLP  STGKVQKFV+R KAKAL 
Sbjct: 421 CAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALV 471

BLAST of CcUC10G186320 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 375.9 bits (964), Expect = 5.3e-104
Identity = 219/569 (38.49%), Postives = 328/569 (57.64%), Query Frame = 0

Query: 1   MDAIFHCPANFAPLSPIRFLERAATVYGCRPSLVYGARVFTWSQTYARCLSLASVLVHHF 60
           MD +  C AN  PL+PI FL+RA+  Y  R S++YG   FTW QTY RC  LA+ L+   
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SL 60

Query: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDTPTLSLLLQQLHPKIIFLDS 120
           ++   D+V  +APN P +YE+HFAVPMAGA+++ +NT+LD  +++ +L+   PKI+F+  
Sbjct: 61  NIGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYR 120

Query: 121 QFLPVL-----LKSLANNSIKFPALVLIPTDPDTPPSLEFLDYNKVLTMRFGDGGFTPKL 180
            F P+      L S  ++++  P + +   D     S E  DY  ++      G  TP L
Sbjct: 121 SFEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQR----GEPTPLL 180

Query: 181 NA-------ELDPISINYTSGSTGLHKGAIYSHRAAYLNSLATIFRSRICSNTSSPVFLW 240
            A       E DPIS+NYTSG+T   KG + SHR AYL++L+ I    +    + PV+LW
Sbjct: 181 LARMFCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEM---GTCPVYLW 240

Query: 241 TVDMFRCNGWCFIWVMAALGGCNICLGTVTSDAIFTSVELHKVTLLCGPSTLLKMISESS 300
           T+ MF CNGW F W  AA GG ++C+  VT+  I+ ++E+H VT +C   T+  ++ + +
Sbjct: 241 TLPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGN 300

Query: 301 SNHYTPRRLSRRVDLIVAGALPIKEILTKVDEMGFNISYGYGMTEAMGPAIIRPWKPSLE 360
           S   + R  S  V ++  G+ P   ++ KV  +GF + + YG+TEA GP +   W+    
Sbjct: 301 SLDLSHR--SGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWN 360

Query: 361 E--ENVQFE-------NLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMCGYYKNL 420
              EN Q E       +++   E+DV++  + ESV  DG+T+GE++++G+++M GY KN 
Sbjct: 361 RLPENQQMELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNP 420

Query: 421 KATHEAFSGDNWYRTGDVGVRHNSGRIEMKDRAKDIVVRADREGAVVSTVEVEAVLMSHP 480
           KAT+EAF    W  +GDVGV H  G +E+KDR+KDI++     G  +S+VEVE ++  +P
Sbjct: 421 KATYEAFK-HGWLNSGDVGVIHPDGHVEIKDRSKDIIISG---GENISSVEVENIIYKYP 480

Query: 481 NVAEAAVVG------ERTLCGFVKLKNGSRENGD----------EIIEFCGMHLPEFMVP 532
            V E AVV         T C FV L+ G   N D          ++IE+C  +LP FM P
Sbjct: 481 KVLETAVVAMPHPTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCP 540