CcUC08G149350 (gene) Watermelon (PI 537277) v1

BLAST of CcUC08G149350 vs. NCBI nr
Match: XP_038884116.1 (uncharacterized protein LOC120075040 [Benincasa hispida])

HSP 1 Score: 521.9 bits (1343), Expect = 4.9e-144
Identity = 293/360 (81.39%), Postives = 313/360 (86.94%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+LA++RLSILT QRRVRCSQA+SDVLQLLQLP+HHRALLRVEKVI DQN
Sbjct: 12  NFRASKFRPLLNLALSRLSILTAQRRVRCSQANSDVLQLLQLPNHHRALLRVEKVIKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGL+FAASRCGDFPELHEIKSVLT
Sbjct: 72  ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIKSVLT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
            RFGKEFTARAVELRNNCGVNHLLMQKLSTRQP+LESRMEVLKAIASENGIVLQID   P
Sbjct: 132 TRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLESRMEVLKAIASENGIVLQIDNHPP 191

Query: 239 SNEETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAA 298
           SNEE L RNTRQNQ E  SEV EN QF TEV SGS+QKYKDVADAAQAAFESAAQAAAAA
Sbjct: 192 SNEEKLTRNTRQNQPEPRSEVVENLQFSTEVASGSKQKYKDVADAAQAAFESAAQAAAAA 251

Query: 299 RAAMELSRSHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKIEYRIRREGEGEEEVNN 358
           RAAMELSRSHDGPSSP SPGSGTTPH+KQE+E+ +VESKP P+ E R RR G G EE   
Sbjct: 252 RAAMELSRSHDGPSSPKSPGSGTTPHDKQELEKAEVESKPNPETENRNRRGGGGGEEEEE 311

Query: 359 SVPGASSPSGEFC--YGEKEKS--DMETKLEKTELSEKASFRLNLEKQPISVRTRRVRGY 415
              G      E     G K K+  ++E K+EKTE+SEK SFRLNLEK+PISVRTRRVRG+
Sbjct: 312 EEEGNKMRGDEMDEERGSKVKNGLNVERKVEKTEVSEKGSFRLNLEKKPISVRTRRVRGH 371

BLAST of CcUC08G149350 vs. NCBI nr
Match: XP_011656519.1 (uncharacterized protein LOC101211044 [Cucumis sativus] >KGN45949.1 hypothetical protein Csa_005294 [Cucumis sativus])

HSP 1 Score: 461.5 bits (1186), Expect = 7.9e-126
Identity = 268/359 (74.65%), Postives = 298/359 (83.01%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+L+++RLSILT QRRV CSQA+SDVLQLLQL HHHRALLRVEKVI DQN
Sbjct: 12  NFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVEKVIKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGL+FAASRCGDFPELHEIKSVLT
Sbjct: 72  ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIKSVLT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
            RFGKEFTARAVELRNNCGVN  LMQKLSTRQP+LE+RM+ LK+IASENGIVLQIDQ   
Sbjct: 132 TRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMDALKSIASENGIVLQIDQLPS 191

Query: 239 SNEETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAA 298
           S +E + RN RQ++AE  SE     +F  EV SGS+  YKDVADAAQAAFESAAQAAAAA
Sbjct: 192 SKQEKVGRNGRQSEAEGQSE-----EFSNEVASGSKTTYKDVADAAQAAFESAAQAAAAA 251

Query: 299 RAAMELSRSHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKI-EYRIRR--EGEGEEE 358
           RAAMELSRSH+GPSSP+ PGSGTT  NKQ+ E+ +VESK K ++ EY   R  EGEGEEE
Sbjct: 252 RAAMELSRSHEGPSSPSKPGSGTTSDNKQKKEKREVESKVKQEMEEYGNGRKGEGEGEEE 311

Query: 359 VNNSVPGASSPSGEFCYGEKEKSDMETKLEKTELSEKASFRLNLEKQPISVRTRRVRGY 415
             + +    + +G           METK+EKTE+SEK SFRLNLEK+PISVRTRRV GY
Sbjct: 312 EGDGMDEERTSNG---------LKMETKVEKTEVSEKGSFRLNLEKKPISVRTRRVGGY 356

BLAST of CcUC08G149350 vs. NCBI nr
Match: XP_022921602.1 (uncharacterized protein LOC111429814 [Cucurbita moschata] >KAG7032508.1 IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461.5 bits (1186), Expect = 7.9e-126
Identity = 269/376 (71.54%), Postives = 304/376 (80.85%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+LA++RL+ILT QRR+R SQA SDVLQLLQL H  RALLRVE+VI DQN
Sbjct: 12  NFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRALLRVEQVIKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVLIEGYLNLL+ERT+LLEQQ +CPEELKEA+AGL+FAASRCGDFPELHEIKSVLT
Sbjct: 72  ALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDFPELHEIKSVLT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
           +RFGKEFTARAVELRNNCGVNHLLMQKLSTR P+LESRM++LK IASENGI LQ+D+   
Sbjct: 132 SRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASENGITLQLDETPL 191

Query: 239 SNEETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAA 298
           SNE   ARN+RQNQ+E H  V EN +F TEVPSGS+QKYKDVADAAQAAFESAAQAAAAA
Sbjct: 192 SNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAAFESAAQAAAAA 251

Query: 299 RAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKIEYRIRREGEG-- 358
           RAAMELSRS     DGPSSP  PGS TT ++KQE ++ +VE+KPK +IEY   +E EG  
Sbjct: 252 RAAMELSRSESQDPDGPSSP-GPGSATTSYHKQEQKKSEVEAKPKSEIEYEYGKEEEGSK 311

Query: 359 ---EEEVNNSVPGASSPSGEFCYGEKEK-----------SDMETKLEKTELSEKASFRLN 415
              E EV NS+ G SS    F    +E             +ME  LEKTE++EK SFRLN
Sbjct: 312 IRAEAEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEMEANLEKTEITEKPSFRLN 371

BLAST of CcUC08G149350 vs. NCBI nr
Match: KAG6601794.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 459.9 bits (1182), Expect = 2.3e-125
Identity = 269/376 (71.54%), Postives = 303/376 (80.59%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+LAV+RL+ILT QRR+R SQA SDVLQLLQL H  RALLRVE+VI DQN
Sbjct: 12  NFRASKFRPLLNLAVSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRALLRVEQVIKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVLIEGYLNLL+ER +LLEQ+ ECPEELKEA+AGL+FAASRCGDFPELHEIKSVLT
Sbjct: 72  ALDAYVLIEGYLNLLIERASLLEQERECPEELKEALAGLLFAASRCGDFPELHEIKSVLT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
           +RFGKEFTARAVELRNNCGVNHLLMQKLSTR P+LESRM++LK IASENGI LQ+D+   
Sbjct: 132 SRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASENGITLQLDETPL 191

Query: 239 SNEETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAA 298
           SNE   ARN+RQNQ+E H  V EN +F TEVPSGS+QKYKDVADAAQAAFESAAQAAAAA
Sbjct: 192 SNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAAFESAAQAAAAA 251

Query: 299 RAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKIEYRIRREGEG-- 358
           RAAMELSRS     DGPSSP  PGS TT ++KQE ++ +VE+KPK +IEY   +E EG  
Sbjct: 252 RAAMELSRSESQDPDGPSSP-GPGSATTSYHKQEQKKSEVEAKPKSEIEYEYGKEEEGSK 311

Query: 359 ---EEEVNNSVPGASSPSGEFCYGEKEK-----------SDMETKLEKTELSEKASFRLN 415
              E EV NS+ G SS    F    +E             +ME  LEKTE++EK SFRLN
Sbjct: 312 IRAEAEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEMEANLEKTEITEKPSFRLN 371

BLAST of CcUC08G149350 vs. NCBI nr
Match: XP_023538782.1 (uncharacterized protein LOC111799606 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 456.1 bits (1172), Expect = 3.3e-124
Identity = 268/376 (71.28%), Postives = 301/376 (80.05%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+LA++RL+ILT QRR+R SQA SDVLQLLQL H  RALLRVE+VI DQN
Sbjct: 12  NFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLRHQQRALLRVEQVIKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVLIEGYLNLL+ERT+LLEQ+ ECPEELKEA+AGL+FAASRCGDFPELHEIKSVLT
Sbjct: 72  ALDAYVLIEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDFPELHEIKSVLT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
           +RFGKEFTARAVELRNNCGVNHLLMQKLSTRQP+LESRM++LK IASENGI LQ+D+   
Sbjct: 132 SRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASENGITLQLDETPL 191

Query: 239 SNEETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAA 298
           SNE   ARN+ QNQ E H  V EN +  TEVPSGS+QKYKDVADAAQAAFESAAQAAAAA
Sbjct: 192 SNE---ARNSSQNQPEPHGVVGENLKLSTEVPSGSKQKYKDVADAAQAAFESAAQAAAAA 251

Query: 299 RAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKIEYRIRREGEG-- 358
           RAAMELSR      DGPSSP  PGSGTT ++KQE ++  VE+KPK +IEY   RE EG  
Sbjct: 252 RAAMELSRPESQDPDGPSSP-GPGSGTTSYDKQEQKKSDVEAKPKLEIEYEYGREEEGSK 311

Query: 359 ---EEEVNNSVPGASSPSGEFCYGEKEK-----------SDMETKLEKTELSEKASFRLN 415
              E EV NS+ G SS    F    +E             +ME  LEKTE++EK SFRLN
Sbjct: 312 IRAEAEVKNSMCGCSSNRELFVKENREMDEQRASNIEIGEEMEANLEKTEITEKPSFRLN 371

BLAST of CcUC08G149350 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.9e-11
Identity = 51/173 (29.48%), Postives = 88/173 (50.87%), Query Frame = 0

Query: 58  PNFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQ 117
           P++ + K +  L LAV+R+ IL  ++         +V +LL+  +   A +RVE +I D+
Sbjct: 6   PSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDE 65

Query: 118 NALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVL 177
             ++ + +IE    LL  R  L+   +E P E+KE++  L++++ R    PEL +IK+ L
Sbjct: 66  YLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQL 125

Query: 178 TNRFGKEFTARAVELRNNC----GVNHLLMQKLSTRQPSLESRMEVLKAIASE 227
             ++GK      +E   NC     VN  ++ KLS   P      + L  IA +
Sbjct: 126 KAKYGK-----GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEK 172

BLAST of CcUC08G149350 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.3e-09
Identity = 46/153 (30.07%), Postives = 74/153 (48.37%), Query Frame = 0

Query: 60  FRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQNA 119
           F+A + R  L L + RL +L  ++     +A  ++   L      RA +RVE +I +   
Sbjct: 6   FKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYL 65

Query: 120 LDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRC-GDFPELHEIKSVLT 179
           ++A  ++E Y +LLL R  L++   E    L E+V+ LI+AA R   +  EL  +   L 
Sbjct: 66  VEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLC 125

Query: 180 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 212
            ++ KE+  +         VN  LM KLS   P
Sbjct: 126 AKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of CcUC08G149350 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.3e-09
Identity = 46/153 (30.07%), Postives = 74/153 (48.37%), Query Frame = 0

Query: 60  FRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQNA 119
           F+A + R  L L + RL +L  ++     +A  ++   L      RA +RVE +I +   
Sbjct: 6   FKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYL 65

Query: 120 LDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRC-GDFPELHEIKSVLT 179
           ++A  ++E Y +LLL R  L++   E    L E+V+ LI+AA R   +  EL  +   L 
Sbjct: 66  VEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLC 125

Query: 180 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 212
            ++ KE+  +         VN  LM KLS   P
Sbjct: 126 AKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of CcUC08G149350 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.3e-09
Identity = 46/153 (30.07%), Postives = 74/153 (48.37%), Query Frame = 0

Query: 60  FRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQNA 119
           F+A + R  L L + RL +L  ++     +A  ++   L      RA +RVE +I +   
Sbjct: 6   FKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYL 65

Query: 120 LDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRC-GDFPELHEIKSVLT 179
           ++A  ++E Y +LLL R  L++   E    L E+V+ LI+AA R   +  EL  +   L 
Sbjct: 66  VEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLC 125

Query: 180 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 212
            ++ KE+  +         VN  LM KLS   P
Sbjct: 126 AKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of CcUC08G149350 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.1e-08
Identity = 45/152 (29.61%), Postives = 73/152 (48.03%), Query Frame = 0

Query: 61  RASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQNAL 120
           +A + R  L L + RL +L  ++     +A  ++   L      RA +RVE +I +   +
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 121 DAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRC-GDFPELHEIKSVLTN 180
           +A  ++E Y +LLL R  L++   E    L E+V+ LI+AA R   +  EL  +   L  
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 181 RFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 212
           ++ KE+  +         VN  LM KLS   P
Sbjct: 127 KYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of CcUC08G149350 vs. ExPASy TrEMBL
Match: A0A0A0KAU0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 3.8e-126
Identity = 268/359 (74.65%), Postives = 298/359 (83.01%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+L+++RLSILT QRRV CSQA+SDVLQLLQL HHHRALLRVEKVI DQN
Sbjct: 12  NFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVEKVIKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGL+FAASRCGDFPELHEIKSVLT
Sbjct: 72  ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIKSVLT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
            RFGKEFTARAVELRNNCGVN  LMQKLSTRQP+LE+RM+ LK+IASENGIVLQIDQ   
Sbjct: 132 TRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMDALKSIASENGIVLQIDQLPS 191

Query: 239 SNEETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAA 298
           S +E + RN RQ++AE  SE     +F  EV SGS+  YKDVADAAQAAFESAAQAAAAA
Sbjct: 192 SKQEKVGRNGRQSEAEGQSE-----EFSNEVASGSKTTYKDVADAAQAAFESAAQAAAAA 251

Query: 299 RAAMELSRSHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKI-EYRIRR--EGEGEEE 358
           RAAMELSRSH+GPSSP+ PGSGTT  NKQ+ E+ +VESK K ++ EY   R  EGEGEEE
Sbjct: 252 RAAMELSRSHEGPSSPSKPGSGTTSDNKQKKEKREVESKVKQEMEEYGNGRKGEGEGEEE 311

Query: 359 VNNSVPGASSPSGEFCYGEKEKSDMETKLEKTELSEKASFRLNLEKQPISVRTRRVRGY 415
             + +    + +G           METK+EKTE+SEK SFRLNLEK+PISVRTRRV GY
Sbjct: 312 EGDGMDEERTSNG---------LKMETKVEKTEVSEKGSFRLNLEKKPISVRTRRVGGY 356

BLAST of CcUC08G149350 vs. ExPASy TrEMBL
Match: A0A6J1E0Z0 (uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC111429814 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 3.8e-126
Identity = 269/376 (71.54%), Postives = 304/376 (80.85%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+LA++RL+ILT QRR+R SQA SDVLQLLQL H  RALLRVE+VI DQN
Sbjct: 12  NFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRALLRVEQVIKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVLIEGYLNLL+ERT+LLEQQ +CPEELKEA+AGL+FAASRCGDFPELHEIKSVLT
Sbjct: 72  ALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDFPELHEIKSVLT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
           +RFGKEFTARAVELRNNCGVNHLLMQKLSTR P+LESRM++LK IASENGI LQ+D+   
Sbjct: 132 SRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASENGITLQLDETPL 191

Query: 239 SNEETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAA 298
           SNE   ARN+RQNQ+E H  V EN +F TEVPSGS+QKYKDVADAAQAAFESAAQAAAAA
Sbjct: 192 SNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAAFESAAQAAAAA 251

Query: 299 RAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKIEYRIRREGEG-- 358
           RAAMELSRS     DGPSSP  PGS TT ++KQE ++ +VE+KPK +IEY   +E EG  
Sbjct: 252 RAAMELSRSESQDPDGPSSP-GPGSATTSYHKQEQKKSEVEAKPKSEIEYEYGKEEEGSK 311

Query: 359 ---EEEVNNSVPGASSPSGEFCYGEKEK-----------SDMETKLEKTELSEKASFRLN 415
              E EV NS+ G SS    F    +E             +ME  LEKTE++EK SFRLN
Sbjct: 312 IRAEAEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEMEANLEKTEITEKPSFRLN 371

BLAST of CcUC08G149350 vs. ExPASy TrEMBL
Match: A0A6J1I2V3 (uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.8e-123
Identity = 265/376 (70.48%), Postives = 300/376 (79.79%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+LAV+RL+ILT QRR+R +QA SDVLQLLQL H  RALLRVE+VI DQN
Sbjct: 12  NFRASKFRPLLNLAVSRLAILTNQRRLRRTQAQSDVLQLLQLHHQQRALLRVEQVIKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVL+EGYLNLL+ERT+LLEQ+ ECPEELKEA+AGL+FAASRCGDFPELHEIKSVLT
Sbjct: 72  ALDAYVLMEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDFPELHEIKSVLT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
             FGKEFTARAVELRNNCGVNHLLMQKLSTRQP+LESRM++LK IASENGI LQ+D+   
Sbjct: 132 THFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASENGITLQLDETPL 191

Query: 239 SNEETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAA 298
           SNE   ARN+RQNQ+E H  V EN +  TEVPSGS+QKYKDVADAAQAAFESAAQAAAAA
Sbjct: 192 SNE---ARNSRQNQSEPHGGVGENLKLSTEVPSGSKQKYKDVADAAQAAFESAAQAAAAA 251

Query: 299 RAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKIEYRIRREGEGEE 358
           RAAMELSRS     DGPSSP  PGS T+ ++KQE ++  VE+KPK +IEY   RE EG +
Sbjct: 252 RAAMELSRSESQDPDGPSSP-GPGSATSSYDKQEQKKSNVEAKPKLEIEYEYGREEEGSK 311

Query: 359 -----EVNNSVPGASSPSGEFCYGEKEK-----------SDMETKLEKTELSEKASFRLN 415
                EV NS+ G SS    F    +E             +ME  LEKT ++EK SFRLN
Sbjct: 312 IRPKPEVKNSMCGCSSNRELFVKENREMDEQRASNIEIGEEMEANLEKTAITEKPSFRLN 371

BLAST of CcUC08G149350 vs. ExPASy TrEMBL
Match: A0A6J1E4E5 (uncharacterized protein LOC111429823 OS=Cucurbita moschata OX=3662 GN=LOC111429823 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 5.9e-119
Identity = 260/376 (69.15%), Postives = 292/376 (77.66%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+L ++RL+ILT QRR+R SQA SDVLQLLQL H  RALLRVE+VI DQN
Sbjct: 12  NFRASKFRPLLNLTLSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRALLRVEQVIKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVLIEGYLNLL+ER +LLEQ+ ECPEELKEA+AGL+FAASRCGDFPELHEIKS LT
Sbjct: 72  ALDAYVLIEGYLNLLIERASLLEQERECPEELKEALAGLLFAASRCGDFPELHEIKSALT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
           +RFGKEFTARAVELRNNCGVNHLLMQKLSTR P+LESRM++LK IASENGI LQ+D+   
Sbjct: 132 SRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASENGITLQLDETPL 191

Query: 239 SNEETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAA 298
           SNE   ARN+RQNQ E    V EN +F TEVPSGS+QKYKDVADAAQAAFESAAQAAAAA
Sbjct: 192 SNE---ARNSRQNQPETDGGVGENLKFSTEVPSGSKQKYKDVADAAQAAFESAAQAAAAA 251

Query: 299 RAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKIEYRIRREGEGEE 358
           RAAMELSR      DGPSSP  PGS TT ++KQ  +E  VE+KPK +IEY   RE EG +
Sbjct: 252 RAAMELSRPESQDPDGPSSP-GPGSATTSYHKQVQKESDVEAKPKLEIEYEYGREEEGSK 311

Query: 359 -----EVNNSVPGASSPSGEFCYGEKEK-----------SDMETKLEKTELSEKASFRLN 415
                EV NS+ G SS    F    +E             +ME  LEK E++EK SF LN
Sbjct: 312 VTPKPEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEMEANLEKAEITEKPSFGLN 371

BLAST of CcUC08G149350 vs. ExPASy TrEMBL
Match: A0A6J1CFG6 (uncharacterized protein LOC111010325 OS=Momordica charantia OX=3673 GN=LOC111010325 PE=3 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.9e-117
Identity = 260/378 (68.78%), Postives = 301/378 (79.63%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           NFRASKFRPLL+LA++RL++LT QR VR SQA SD LQLLQL HHHRALLRVE+VI +QN
Sbjct: 12  NFRASKFRPLLNLALSRLAVLTNQRHVRRSQARSDALQLLQLGHHHRALLRVEQVIKEQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
           ALDAYVLIEGYLNLL+ERT LLEQ+ ECPEELKEAV+GLIFAASRCGDFPEL EIKSVLT
Sbjct: 72  ALDAYVLIEGYLNLLIERTDLLEQERECPEELKEAVSGLIFAASRCGDFPELQEIKSVLT 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
            RFGKEFTARAVELRNNCGV+H +MQKLSTRQP+LESRM VL+AIASEN IVLQ+D+   
Sbjct: 132 TRFGKEFTARAVELRNNCGVDHSIMQKLSTRQPNLESRMNVLEAIASENNIVLQLDEHPL 191

Query: 239 SNEETLARNTRQN-QAEAHSEVKENFQFFTEVPSGS---QQKYKDVADAAQAAFESAAQA 298
           SNEE  ARN RQN Q E  +EV +N QF  +VPSGS   +QKYKDVADAAQAAFESAAQA
Sbjct: 192 SNEEKFARNRRQNYQQEPKAEVGDNLQFSADVPSGSMQAKQKYKDVADAAQAAFESAAQA 251

Query: 299 AAAARAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKIEYRIRREG 358
           AAAARAAMELSRS     D PS+P  PGSG+T + +QE ++ +VESKPK +IEY+ RRE 
Sbjct: 252 AAAARAAMELSRSQSQDPDDPSTP-KPGSGST-NKRQEQKKSEVESKPKQEIEYQNRREE 311

Query: 359 EG-----EEEVNNSVPGASSPSGEFCYGEKEKS---------DMETKLEKTELSEKASFR 415
           E        EV NS+P + SPS E    E++++         + ETK E TE ++K SF 
Sbjct: 312 EEAGSKITAEVKNSMPASFSPSREDSEMEEQRAPNVETGLDMETETKPELTEETQKPSFG 371

BLAST of CcUC08G149350 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 229.6 bits (584), Expect = 4.7e-60
Identity = 141/281 (50.18%), Postives = 184/281 (65.48%), Query Frame = 0

Query: 59  NFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQN 118
           +F+ +KF+ L++LA+TRLSIL  QR+ R SQA SDV +LL+L  H  A  RV++V+ DQN
Sbjct: 12  SFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAYHRVDQVVKDQN 71

Query: 119 ALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLT 178
            LD    I GY  L L+R  L E   +CPEEL EAV+GL+FAASR G+FPEL EI++VL 
Sbjct: 72  TLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEFPELQEIRNVLI 131

Query: 179 NRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYP 238
           +RFGK+  AR++ELR+NCGV+  ++QKLSTR P  E RM+ LK IA+EN IVL++DQ   
Sbjct: 132 SRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAAENNIVLKLDQAST 191

Query: 239 SNEETLARNTRQNQAEAHSEVK----ENFQFFTEVPSGSQQKYKDVADAAQAAFESAAQA 298
           S E T       + ++     K    E +     V  G ++KYKDVADAAQAAFESAA A
Sbjct: 192 STEGTTNMQGTSDVSKTKLTSKDGRGEGYGLSDSVKRG-KKKYKDVADAAQAAFESAAHA 251

Query: 299 AAAARAAMELS----RSHDGPSSPNSPGSGTTPHNKQEVEE 332
           A AA+AA+ELS    R HD P +     S     NK+  +E
Sbjct: 252 AEAAQAAVELSQFSPRGHDSPGNIGGENSFHGSENKESEQE 291

BLAST of CcUC08G149350 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 125.9 bits (315), Expect = 7.3e-29
Identity = 114/387 (29.46%), Postives = 177/387 (45.74%), Query Frame = 0

Query: 41  ASSSTLLHTALFSTLKQPNFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQL 100
           AS+ T        +L +  F +SK +    +AV R+ ++  +R V   Q   D+  LLQ 
Sbjct: 7   ASAQTKKLMKFSLSLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQS 66

Query: 101 PHHHRALLRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFA 160
                A +RVE VI +QN   A  +IE +  L++ R T++ +Q +CP +LKE +A LIFA
Sbjct: 67  GQDATARIRVEHVIREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFA 126

Query: 161 ASRCGDFPELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVL 220
           A RC + PEL +++ +   ++GK+F + A +LR +CGVN +L+ KLS R P  E +++++
Sbjct: 127 APRCSEIPELGDLRDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIM 186

Query: 221 KAIASENGIVLQIDQPYPSNEETLARNTRQN--------QAEA----HSEVKENFQFFTE 280
           K IA E     Q+D      E+ L +   ++         A +     + + E       
Sbjct: 187 KEIAKE----FQVDWDTTETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKA 246

Query: 281 VPSGSQQ-----KYKDVADAAQAAFESAAQAAAAARAAMELSRSHDGPSSPNSPGSGTTP 340
           VP  +        Y D   AA+AA E A QA AAA+ A  L+   D  +   S  S  + 
Sbjct: 247 VPRSTSSMSINTHYHDTESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHST 306

Query: 341 HNKQEVEEPKVESKPKPKIEYRIRREGEGEEEVNNSVPGASS--PSGEFCYGEK--EKSD 400
           H K           P  +     R+  + E     + PGA +        Y      +SD
Sbjct: 307 HQKDSQYMDHHHHHPGSR-----RQSRDSETSSYYAKPGAENRGMGRRHSYNNPGINESD 366

Query: 401 METKLEKTELSEKASFRLNLEKQPISV 407
            E +   TE   K + R      P SV
Sbjct: 367 YEEEYTNTEAEAKETMRRRHSYNPRSV 384

BLAST of CcUC08G149350 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.2e-28
Identity = 67/174 (38.51%), Postives = 107/174 (61.49%), Query Frame = 0

Query: 57  QPNFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVD 116
           Q  F+ +K +  L +A +RL IL  ++ ++  Q   ++ QLL+      A +RVE V+ +
Sbjct: 6   QRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVRE 65

Query: 117 QNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSV 176
           +  + AY LI  Y  LL+ R  ++E Q  CP +LKEAV  ++FA+ R  D PEL EI   
Sbjct: 66  EKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQ 125

Query: 177 LTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIV 231
            T ++GK+F+  AVELR + GV+ LL++KLS + P   +++++L AIA E+ +V
Sbjct: 126 FTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVV 179

BLAST of CcUC08G149350 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 115.5 bits (288), Expect = 9.9e-26
Identity = 90/316 (28.48%), Postives = 146/316 (46.20%), Query Frame = 0

Query: 50  ALFSTLKQPNFRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLR 109
           +L + L       +K +  L+LA+ R+ +L  +R ++      ++   LQ      A +R
Sbjct: 2   SLLNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIR 61

Query: 110 VEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPE 169
           VE VI + N   AY ++E +   +L R  +LE + ECP EL+EA+A +IFAA RC + P+
Sbjct: 62  VEHVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPD 121

Query: 170 LHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGI 229
           L +IK++   ++GKEF   A ELR + GVN  +++KLS   PS  +R+++LK IA E  +
Sbjct: 122 LLQIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSL 181

Query: 230 VLQ---------------------------IDQPYPSNEETLARNTRQNQAEAHSEVKEN 289
                                         I +  PS +     +  +      +E  + 
Sbjct: 182 NWDSSATEAEFMKSHEDLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQR 241

Query: 290 FQFFT------------------EVPSGSQQKYKDVADAAQAAFESAAQAAAAARAAMEL 321
           FQ                     + P  +++   DV + A+AA  SA +A AAARAA +L
Sbjct: 242 FQKLQAQNPVSKSMPSSKLTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQL 301

BLAST of CcUC08G149350 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 108.2 bits (269), Expect = 1.6e-23
Identity = 84/291 (28.87%), Postives = 144/291 (49.48%), Query Frame = 0

Query: 60  FRASKFRPLLSLAVTRLSILTTQRRVRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQNA 119
           F+A+K + LL L + R+ ++  +R  +  Q   ++ +LL+      A +RVE +I ++  
Sbjct: 12  FKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKM 71

Query: 120 LDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLTN 179
           + A  ++E +  L+  R  ++E Q ECP +LKEA++ + FAA RC D  EL +++ +  +
Sbjct: 72  MAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVS 131

Query: 180 RFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYPS 239
           ++GKEF A A EL+ + GVN  L++ LS R PS E+++++LK IA E+    ++D    S
Sbjct: 132 KYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEH----ELDWDPAS 191

Query: 240 NE-------ETLARNTRQNQAEAHSEVKENFQFFTEVPSGSQQKYKDVADAAQAAFE--- 299
            E       E L    +Q    +   + E     T + S S  K K  +D+     +   
Sbjct: 192 TETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKTNLTSLSAAKEKSDSDSEYDILDFPE 251

Query: 300 ------------SAAQAAAAARAAMELSRSHDGPSSPNSPGSGTTPHNKQE 329
                       ++  A  AA++A     SHD P    + G   T   + E
Sbjct: 252 VPNVLLRPTPGATSVNAPDAAKSASYEHTSHDLPFDSENAGVEKTASKRDE 298