CcUC08G148700 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC08G148700
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	rRNA N-glycosidase
Location	CicolChr08: 13210271 .. 13211122 (-)
RNA-Seq Expression	CcUC08G148700
Synteny	CcUC08G148700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTGATTGAATCCTCTTCCTTAAATTACAGTGCTCGTGCTGCCAACCATGCCGATCGTCGTCCTGCCGATTCGAATCTCAGCTTCTCTCTGGTTGGTGCAACTGCTTCTACTTACGAACAATTCATAGAAGATCTACGTAAGGCACTTACACTGAACTCCCACTCAGTCTACAACATACCTGTCTTAGCATCCACAGCCAAAGGGCTCAACCGCTTCATATTGCTCAATCTCACCAATTACCAAGCCGAATCCATCACTCTCGCAGTTGACGTAGTTGATGTTTACATAGTTGCATACCAAGCCCAAAACAGAGCCTATTTCCTCAAGAACGTTTCCAAGGAAGCACATGATGTGTTATTTAAAGGTATGAAACATGAAACACTTCCTTATAAAGGGAATTACGATGACCTCGAGAGATGTGCTGGAAAGATTTCGAGAGAGAATATTGATCTTGGATTATCTGAACTAGGGTGCGCCATTGGAAATCTGTTCCATCATAATCCTGGGAATTCTGTGCCAAAAGCATTCATTGTGATCATTCAAACGATTTCAGAAGCTGCAAGATTTAAGTATATTGAGTCAAAGTATAATTGGGATAAGTTTAAACCTGACCCTGCATTCATAAGCTTAGAAAATCGTTGGTCCGATCTCTCGGAACAGATACAGAAGGCGGAAAAGCAAGGAGGGAAATTTAAGGCTCCAGTTCCGGTTCGAAATGTTAAGAATGAAGTAATTATGGTAAGTGATGTTAATTCGCCAGTCGTTAAAGGGATTGCGCTATTACTTCCCGATAAGCTGGCAGAAGCACAAAATGGAAATCTGAAACAAATGGAAGAGGTTAATTAA

mRNA sequence

ATGGAGTTGATTGAATCCTCTTCCTTAAATTACAGTGCTCGTGCTGCCAACCATGCCGATCGTCGTCCTGCCGATTCGAATCTCAGCTTCTCTCTGGTTGGTGCAACTGCTTCTACTTACGAACAATTCATAGAAGATCTACGTAAGGCACTTACACTGAACTCCCACTCAGTCTACAACATACCTGTCTTAGCATCCACAGCCAAAGGGCTCAACCGCTTCATATTGCTCAATCTCACCAATTACCAAGCCGAATCCATCACTCTCGCAGTTGACGTAGTTGATGTTTACATAGTTGCATACCAAGCCCAAAACAGAGCCTATTTCCTCAAGAACGTTTCCAAGGAAGCACATGATGTGTTATTTAAAGGTATGAAACATGAAACACTTCCTTATAAAGGGAATTACGATGACCTCGAGAGATGTGCTGGAAAGATTTCGAGAGAGAATATTGATCTTGGATTATCTGAACTAGGGTGCGCCATTGGAAATCTGTTCCATCATAATCCTGGGAATTCTGTGCCAAAAGCATTCATTGTGATCATTCAAACGATTTCAGAAGCTGCAAGATTTAAGTATATTGAGTCAAAGTATAATTGGGATAAGTTTAAACCTGACCCTGCATTCATAAGCTTAGAAAATCGTTGGTCCGATCTCTCGGAACAGATACAGAAGGCGGAAAAGCAAGGAGGGAAATTTAAGGCTCCAGTTCCGGTTCGAAATGTTAAGAATGAAGTAATTATGGTAAGTGATGTTAATTCGCCAGTCGTTAAAGGGATTGCGCTATTACTTCCCGATAAGCTGGCAGAAGCACAAAATGGAAATCTGAAACAAATGGAAGAGGTTAATTAA

Coding sequence (CDS)

ATGGAGTTGATTGAATCCTCTTCCTTAAATTACAGTGCTCGTGCTGCCAACCATGCCGATCGTCGTCCTGCCGATTCGAATCTCAGCTTCTCTCTGGTTGGTGCAACTGCTTCTACTTACGAACAATTCATAGAAGATCTACGTAAGGCACTTACACTGAACTCCCACTCAGTCTACAACATACCTGTCTTAGCATCCACAGCCAAAGGGCTCAACCGCTTCATATTGCTCAATCTCACCAATTACCAAGCCGAATCCATCACTCTCGCAGTTGACGTAGTTGATGTTTACATAGTTGCATACCAAGCCCAAAACAGAGCCTATTTCCTCAAGAACGTTTCCAAGGAAGCACATGATGTGTTATTTAAAGGTATGAAACATGAAACACTTCCTTATAAAGGGAATTACGATGACCTCGAGAGATGTGCTGGAAAGATTTCGAGAGAGAATATTGATCTTGGATTATCTGAACTAGGGTGCGCCATTGGAAATCTGTTCCATCATAATCCTGGGAATTCTGTGCCAAAAGCATTCATTGTGATCATTCAAACGATTTCAGAAGCTGCAAGATTTAAGTATATTGAGTCAAAGTATAATTGGGATAAGTTTAAACCTGACCCTGCATTCATAAGCTTAGAAAATCGTTGGTCCGATCTCTCGGAACAGATACAGAAGGCGGAAAAGCAAGGAGGGAAATTTAAGGCTCCAGTTCCGGTTCGAAATGTTAAGAATGAAGTAATTATGGTAAGTGATGTTAATTCGCCAGTCGTTAAAGGGATTGCGCTATTACTTCCCGATAAGCTGGCAGAAGCACAAAATGGAAATCTGAAACAAATGGAAGAGGTTAATTAA

Protein sequence

MELIESSSLNYSARAANHADRRPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTAKGLNRFILLNLTNYQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHETLPYKGNYDDLERCAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEAARFKYIESKYNWDKFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNEVIMVSDVNSPVVKGIALLLPDKLAEAQNGNLKQMEEVN

Homology

[Close]

BLAST of CcUC08G148700 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 371.7 bits (953), Expect = 5.6e-99
Identity = 194/276 (70.29%), Postives = 229/276 (82.97%), Query Frame = 0


Query: 9   LNYSARAANHADRRPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTA 68
           LN  A  A  A R  A++NLSFSL+GATA TY+QFI DLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATA 76

Query: 69  KGLNRFILLNLTNYQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHE 128
           KGL RFIL+NLTNY+ ESIT+A+D VDVYIVAYQA N+AYFLK+VSKEAHDVLFKG KHE
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 129 TLPYKGNYDDLERCAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEA 188
           TL YKGNYDDLER AGKISRENIDLG SEL  +IGN+FH+N G SVPK+FIV+IQ +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEA 196

Query: 189 ARFKYIESKYNWD---KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNE 248
           +RFKYIE++   +   KFKPDP ++SLENRWS+LSEQ+QKA+K GGKF+ PV VR+V N+
Sbjct: 197 SRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256

Query: 249 VIMVSDVNSPVVKGIALLLPDKLAEAQNGNLKQMEE 282
            ++VSDVNSPVVKG+ALLL +K     NG  K MEE
Sbjct: 257 PVLVSDVNSPVVKGMALLLCNK--PGVNGVSKLMEE 290

[Close]

BLAST of CcUC08G148700 vs. NCBI nr
Match: KAG7037232.1 (hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 371.7 bits (953), Expect = 5.6e-99
Identity = 195/277 (70.40%), Postives = 231/277 (83.39%), Query Frame = 0


Query: 9   LNYSARAANHADRRPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTA 68
           LN  A AA  A R  A++NLSFSL+GATA TY+QFI DLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76

Query: 69  KGLNRFILLNLTNYQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHE 128
           KGL RFIL+NLTNY+ ESIT+A+D VDVYIVAYQA N+AYFLK+VSKEAHDVLFKG KHE
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 129 TLPYKGNYDDLERCAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEA 188
           TL YKGNYDDLER AGKISRENI+LG SEL  +IGN+FH+N G SVP++FIV+IQ +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEA 196

Query: 189 ARFKYIESKYNWD----KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKN 248
           +RFKYIE++ N +    KFKPDP ++SLENRWS+LSEQ+QKA+K GGKF+ PV VR+V N
Sbjct: 197 SRFKYIETR-NCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSN 256

Query: 249 EVIMVSDVNSPVVKGIALLLPDKLAEAQNGNLKQMEE 282
           + ++VSDVNSPVVKGIALLL +K     NG  K MEE
Sbjct: 257 KPVLVSDVNSPVVKGIALLLCNK--PGVNGVSKLMEE 290

[Close]

BLAST of CcUC08G148700 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 370.2 bits (949), Expect = 1.6e-98
Identity = 194/277 (70.04%), Postives = 230/277 (83.03%), Query Frame = 0


Query: 9   LNYSARAANHADRRPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTA 68
           LN  A AA  A R  A++NLSFSL+GATA TY+QFI DLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76

Query: 69  KGLNRFILLNLTNYQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHE 128
           KGL RFIL+NLTNY+ ESIT+A+D VDVYIVAYQA N+AYFLK+VSKEAHDVLFKG KHE
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 129 TLPYKGNYDDLERCAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEA 188
           TL YKGNYDDLER AGKISRENI+LG SEL  +IGN+FH+  G SVP++FIV+IQ +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEA 196

Query: 189 ARFKYIESKYNWD----KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKN 248
           +RFKYIE++ N +    KFKPDP ++SLENRWS+LSEQ+QKA+K GGKF+ PV VR+V N
Sbjct: 197 SRFKYIETR-NCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSN 256

Query: 249 EVIMVSDVNSPVVKGIALLLPDKLAEAQNGNLKQMEE 282
           + ++VSDVNSPVVKGIALLL +K     NG  K MEE
Sbjct: 257 KPVLVSDVNSPVVKGIALLLSNK--PGVNGVSKLMEE 290

[Close]

BLAST of CcUC08G148700 vs. NCBI nr
Match: KAG6607631.1 (hypothetical protein SDJN03_00973, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 365.5 bits (937), Expect = 4.0e-97
Identity = 188/263 (71.48%), Postives = 223/263 (84.79%), Query Frame = 0


Query: 9   LNYSARAANHADRRPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTA 68
           LN  A AA  A R  A++NLSFSL+GATA TY+QFI DLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76

Query: 69  KGLNRFILLNLTNYQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHE 128
           KGL RFIL+NLTNY+ ESIT+A+D VDVYIVAYQA N+AYFLK+VSKEAHDVLFKG KHE
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 129 TLPYKGNYDDLERCAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEA 188
           TL YKGNYDDLER AGKISRENI+LG SEL  +IGN+FH+N G SVP++FIV+IQ +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEA 196

Query: 189 ARFKYIESKYNWD----KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKN 248
           +RFKYIE++ N +    KFKPDP ++SLENRWS+LSE +QKA+K GGKF+ PV VR+V N
Sbjct: 197 SRFKYIETR-NCEKVESKFKPDPGYLSLENRWSNLSEHVQKAQKHGGKFERPVQVRSVSN 256

Query: 249 EVIMVSDVNSPVVKGIALLLPDK 268
           + I V+DVNSPVVKGIALLL +K
Sbjct: 257 KPIKVTDVNSPVVKGIALLLCNK 278

[Close]

BLAST of CcUC08G148700 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362.1 bits (928), Expect = 4.5e-96
Identity = 191/277 (68.95%), Postives = 226/277 (81.59%), Query Frame = 0


Query: 9   LNYSARAANHADRRPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTA 68
           LN  A AA  A R  A++NLSFSL+GATA TY+QFI DLR ALT+ SH VY IPVL +TA
Sbjct: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRNALTVQSHKVYGIPVLPATA 76

Query: 69  KGLNRFILLNLTNYQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHE 128
           KGL RFIL+NLTNY+ ESIT+A+D VDVYIVAYQA N+AYFLK+VSKEAHDVLFKG KHE
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 129 TLPYKGNYDDLERCAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEA 188
           TL YKGNYDDLER AG ISRENI+LG SEL  +IGN+FH+N G SVP++FIV+IQ +SEA
Sbjct: 137 TLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEA 196

Query: 189 ARFKYIESKYNWD----KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKN 248
           +RFKYIE++ N +    KFKPDP ++SLENRWS+LSEQ+QKA+K GGKF+ PV VR+V N
Sbjct: 197 SRFKYIETR-NCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFERPVQVRSVSN 256

Query: 249 EVIMVSDVNSPVVKGIALLLPDKLAEAQNGNLKQMEE 282
           + I V+DVNSPVVKGIALLL +K     NG  K ME+
Sbjct: 257 KPIKVTDVNSPVVKGIALLLCNK--PGVNGVSKLMEQ 290

[Close]

BLAST of CcUC08G148700 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 8.8e-79
Identity = 149/243 (61.32%), Postives = 188/243 (77.37%), Query Frame = 0


Query: 25  DSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTAKGLNRFILLNLTNYQA 84
           D +++FSL+GAT +TY+ FI +LR  LT+ +  VY+IPVL + A GL RF L+ LTNY  
Sbjct: 21  DVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAAAGLARFQLVTLTNYNG 80

Query: 85  ESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHETLPYKGNYDDLERCAG 144
           ES+T+A+DVV+VY+VAY+A N AYFL + S EA++VLF G+ H  LPY GNYD LE  AG
Sbjct: 81  ESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHVRLPYGGNYDGLETAAG 140

Query: 145 KISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEAARFKYIESKYNWD--- 204
           +ISRENI+LG SE+  AIGN+F HNPG SVP+AFIVIIQT+SEAARFKYIE + + +   
Sbjct: 141 RISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEAARFKYIEQRVSENVGT 200

Query: 205 KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNEVIMVSDVNSPVVKGIA 264
           KFKPDPAF+SL+N W  LSEQIQ A+ +GG+F  PV +R V N    V++VNSPVVKGIA
Sbjct: 201 KFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNTPTFVTNVNSPVVKGIA 260

[Close]

BLAST of CcUC08G148700 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 224.6 bits (571), Expect = 1.5e-57
Identity = 131/260 (50.38%), Postives = 173/260 (66.54%), Query Frame = 0


Query: 24  ADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTAKGLNRFILLNLTNYQ 83
           A  +++F L  ATA TY +FIED R  L   SH VY+IP+L ST     RFILLNLT+Y 
Sbjct: 21  AKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTSYA 80

Query: 84  AESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHETLPYKGNYDDLERCA 143
            E+I++A+DV +VY+VAY+ ++ +YF K    EA+++LFKG +  TLPY GNY++L+  A
Sbjct: 81  YETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQTAA 140

Query: 144 GKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEAARFKYIE---SKYNW 203
            KI RENIDLGL  L  AI  LF++N   S P A +V+IQT +EAARFKYIE   +KY  
Sbjct: 141 HKI-RENIDLGLPALSSAITTLFYYN-AQSAPSALLVLIQTTAEAARFKYIERHVAKYVA 200

Query: 204 DKFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNEVIMVSDVNSPVVKG- 263
             FKP+ A ISLEN+WS LS+QI  A+ QGGKF+ PV +     E   V++V+S VVKG 
Sbjct: 201 TNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVKGN 260

Query: 264 IALLLPDKLAEAQNGNLKQM 280
           I LLL  + + A    +  M
Sbjct: 261 IKLLLNSRASTADENFITTM 277

[Close]

BLAST of CcUC08G148700 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.5e-57
Identity = 130/260 (50.00%), Postives = 173/260 (66.54%), Query Frame = 0


Query: 24  ADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTAKGLNRFILLNLTNYQ 83
           A  +++F L  ATA TY +FIED R  L   SH VY+IP+L ST     RFILL+LT+Y 
Sbjct: 21  AKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLDLTSYA 80

Query: 84  AESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHETLPYKGNYDDLERCA 143
            E+I++A+DV +VY+VAY+ ++ +YF K    EA+++LFKG +  TLPY GNY++L+  A
Sbjct: 81  YETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQTAA 140

Query: 144 GKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEAARFKYIE---SKYNW 203
            KI RENIDLGL  L  AI  LF++N   S P A +V+IQT +EAARFKYIE   +KY  
Sbjct: 141 HKI-RENIDLGLPALSSAITTLFYYN-AQSAPSALLVLIQTTAEAARFKYIERHVAKYVA 200

Query: 204 DKFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNEVIMVSDVNSPVVKG- 263
             FKP+ A ISLEN+WS LS+QI  A+ QGGKF+ PV +     E   V++V+S VVKG 
Sbjct: 201 TNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVKGN 260

Query: 264 IALLLPDKLAEAQNGNLKQM 280
           I LLL  + + A    +  M
Sbjct: 261 IKLLLNSRASTADENFITTM 277

[Close]

BLAST of CcUC08G148700 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 1.1e-49
Identity = 115/242 (47.52%), Postives = 159/242 (65.70%), Query Frame = 0


Query: 27  NLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTAKGLNRFILLNLTNYQAES 86
           N+ F L  AT+S+Y+ FI++LR+AL  +   VY+IPVL ST     RFIL++L NY  +S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDG-KVYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 87  ITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHETLPYKGNYDDLERCAGKI 146
           IT A+DV++VYIVAY     +YF + V  +A  +LFKG +  TLPY GNY++L+  A K+
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 147 SRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEAARFKYIE---SKYNWDKF 206
            RENI+LGL  L  AI  LFH+N   +   A +V+IQT SEAARF+YIE   +     KF
Sbjct: 121 -RENIELGLPALDSAITTLFHYN-AEAAASALLVLIQTTSEAARFRYIELQIANNVGTKF 180

Query: 207 KPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNEVIMVSDVNSPVV-KGIAL 265
           KP    ISLEN WS LS+QIQ A+ + G+F+ PV + + +   + +++V S VV + I L
Sbjct: 181 KPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQL 239

[Close]

BLAST of CcUC08G148700 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 190.3 bits (482), Expect = 3.0e-47
Identity = 117/246 (47.56%), Postives = 164/246 (66.67%), Query Frame = 0


Query: 25  DSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTAKGLNRFILLNLTNYQA 84
           + ++SF L GAT+S+Y  FI +LRKAL  N   +Y+IP+L S+  G  R+ L++LTNY  
Sbjct: 22  EGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSSLPGSQRYALIHLTNYAD 81

Query: 85  ESITLAVDVVDVYIVAYQAQNRAYFLKNVS-KEAHDVLFK-GMKHETLPYKGNYDDLERC 144
           E+I++A+DV +VYI+ Y+A + +YF    S  EA   +FK  M+  TLPY GNY+ L+  
Sbjct: 82  ETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLPYSGNYERLQTA 141

Query: 145 AGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEAARFKYIESKY--NW 204
           AGKI RENI LGL  L  AI  LF++N  NS   A +V+IQ+ SEAAR+K+IE +     
Sbjct: 142 AGKI-RENIPLGLPALDSAITTLFYYN-ANSAASALMVLIQSTSEAARYKFIEQQIGKRV 201

Query: 205 DK-FKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNEVIMVSDVNSPVV-K 264
           DK F P  A ISLEN WS LS+QIQ A    G+F++PV + N +N+ + +++V++ VV  
Sbjct: 202 DKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVTITNVDAGVVTS 261

[Close]

BLAST of CcUC08G148700 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.7e-99
Identity = 194/276 (70.29%), Postives = 229/276 (82.97%), Query Frame = 0


Query: 9   LNYSARAANHADRRPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTA 68
           LN  A  A  A R  A++NLSFSL+GATA TY+QFI DLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATA 76

Query: 69  KGLNRFILLNLTNYQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHE 128
           KGL RFIL+NLTNY+ ESIT+A+D VDVYIVAYQA N+AYFLK+VSKEAHDVLFKG KHE
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 129 TLPYKGNYDDLERCAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEA 188
           TL YKGNYDDLER AGKISRENIDLG SEL  +IGN+FH+N G SVPK+FIV+IQ +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEA 196

Query: 189 ARFKYIESKYNWD---KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNE 248
           +RFKYIE++   +   KFKPDP ++SLENRWS+LSEQ+QKA+K GGKF+ PV VR+V N+
Sbjct: 197 SRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256

Query: 249 VIMVSDVNSPVVKGIALLLPDKLAEAQNGNLKQMEE 282
            ++VSDVNSPVVKG+ALLL +K     NG  K MEE
Sbjct: 257 PVLVSDVNSPVVKGMALLLCNK--PGVNGVSKLMEE 290

[Close]

BLAST of CcUC08G148700 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 7.9e-99
Identity = 194/277 (70.04%), Postives = 230/277 (83.03%), Query Frame = 0


Query: 9   LNYSARAANHADRRPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTA 68
           LN  A AA  A R  A++NLSFSL+GATA TY+QFI DLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76

Query: 69  KGLNRFILLNLTNYQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHE 128
           KGL RFIL+NLTNY+ ESIT+A+D VDVYIVAYQA N+AYFLK+VSKEAHDVLFKG KHE
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 129 TLPYKGNYDDLERCAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEA 188
           TL YKGNYDDLER AGKISRENI+LG SEL  +IGN+FH+  G SVP++FIV+IQ +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEA 196

Query: 189 ARFKYIESKYNWD----KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKN 248
           +RFKYIE++ N +    KFKPDP ++SLENRWS+LSEQ+QKA+K GGKF+ PV VR+V N
Sbjct: 197 SRFKYIETR-NCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSN 256

Query: 249 EVIMVSDVNSPVVKGIALLLPDKLAEAQNGNLKQMEE 282
           + ++VSDVNSPVVKGIALLL +K     NG  K MEE
Sbjct: 257 KPVLVSDVNSPVVKGIALLLSNK--PGVNGVSKLMEE 290

[Close]

BLAST of CcUC08G148700 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 6.9e-87
Identity = 177/272 (65.07%), Postives = 218/272 (80.15%), Query Frame = 0


Query: 16  ANHADRRPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTAKGLNRFI 75
           AN A   P +SN+SFSL+GAT  TY+QFI++LR ALT+NS  VYNIPVLA+TA    RFI
Sbjct: 26  ANGAVNAP-NSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFI 85

Query: 76  LLNLTNYQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHETLPYKGN 135
           L++LTNY+ E+IT+A+DVV+VYIVAYQA N+AYFLK+ SKEA DVLFKG+K E LPYKGN
Sbjct: 86  LVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGN 145

Query: 136 YDDLERCAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEAARFKYIE 195
           YD LE  AGKISRE IDLG SELG AIGN++H+N G SVP+AFIV+IQTISEAARFKYIE
Sbjct: 146 YDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIE 205

Query: 196 SKYNWD---KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNEVIMVSDV 255
           +K + +   KFKPDP F+SLENRWSDLSEQ+Q A+ + G+F  P+ VR+V N+ I+V++V
Sbjct: 206 TKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNV 265

Query: 256 NSPVVKGIALLLPDKLAEAQNGNLKQ-MEEVN 284
            S VV+G+ALLL  K     NG+L + MEE N
Sbjct: 266 KSKVVEGMALLLYSK--PRGNGDLMELMEEFN 294

[Close]

BLAST of CcUC08G148700 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.4e-79
Identity = 155/251 (61.75%), Postives = 199/251 (79.28%), Query Frame = 0


Query: 24  ADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTAKGLNRFILLNLTNYQ 83
           A++++SFS++GAT+ TY QFI +LR  LT+ S  VY IPVL STA G  RFIL++LTNY 
Sbjct: 19  AEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVGSARFILVHLTNYN 78

Query: 84  AESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHETLPYKGNYDDLERCA 143
            ESIT+A+DVV++Y+VAY+A N AYFL + S EA++VLFKG+KH  LPYKGNYD LE  A
Sbjct: 79  TESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRLPYKGNYDGLETAA 138

Query: 144 GKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEAARFKYIESKYNWD-- 203
           GKISRE IDLG SE+  +IGN+FHHN G S+ +AFIVIIQ++ EAARFKYIE + + +  
Sbjct: 139 GKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSVLEAARFKYIEQRVSENVK 198

Query: 204 -KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNEVIMVSDVNSPVVKGI 263
            KFKPDPAF+SLENRWSDLSEQIQ A+K+ G+F   + +R V N+ I+V++V+SP+VKGI
Sbjct: 199 TKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSSPIVKGI 258

Query: 264 ALLLPDKLAEA 272
           ALLL  K+  A
Sbjct: 259 ALLLYYKVRVA 269

[Close]

BLAST of CcUC08G148700 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 4.5e-78
Identity = 154/258 (59.69%), Postives = 204/258 (79.07%), Query Frame = 0


Query: 22  RPADSNLSFSLVGATASTYEQFIEDLRKALTLNSHSVYNIPVLASTAKGLNRFILLNLTN 81
           R A++++SFS++G+T+ TY+QFI+++R ALT+ S  VY+IPVL +TA G  RF+L++LTN
Sbjct: 17  RAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHLTN 76

Query: 82  YQAESITLAVDVVDVYIVAYQAQNRAYFLKNVSKEAHDVLFKGMKHETLPYKGNYDDLER 141
           Y +ESI +A+DVV+VY+VAY+A N AYFL + S EA  VLF+G+ H  L YKGNYD LE 
Sbjct: 77  YNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGLE- 136

Query: 142 CAGKISRENIDLGLSELGCAIGNLFHHNPGNSVPKAFIVIIQTISEAARFKYIESKYNWD 201
            A KISRE IDLG SE+  +IGN+FHHN G SVP+AFIVIIQ++SEAARFKYIE + + +
Sbjct: 137 TAAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVSEN 196

Query: 202 ---KFKPDPAFISLENRWSDLSEQIQKAEKQGGKFKAPVPVRNVKNEVIMVSDVNSPVVK 261
               FKPDPAF+SL+NRWSDLSEQIQ A+K+GG+F   V +R V N+ I+V+DV+SPVVK
Sbjct: 197 VKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPVVK 256

Query: 262 GIALLLPDKLAEAQNGNL 277
           GIALLL  K++   + N+
Sbjct: 257 GIALLLYYKVSVVMDDNI 273

The following BLAST results are available for this feature:

BLAST of CcUC08G148700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022981562.1	5.6e-99	70.29	ribosome-inactivating protein bryodin II-like [Cucurbita maxima]	[more]
KAG7037232.1	5.6e-99	70.40	hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022926255.1	1.6e-98	70.04	ribosome-inactivating protein bryodin II-like [Cucurbita moschata]	[more]
KAG6607631.1	4.0e-97	71.48	hypothetical protein SDJN03_00973, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023523582.1	4.5e-96	68.95	ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CcUC08G148700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	8.8e-79	61.32	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P24817	1.5e-57	50.38	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	5.5e-57	50.00	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P84531	1.1e-49	47.52	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
P09989	3.0e-47	47.56	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]

BLAST of CcUC08G148700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IUB2	2.7e-99	70.29	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]
A0A6J1EKL0	7.9e-99	70.04	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1	[more]
A0A6J1E093	6.9e-87	65.07	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1DWK4	1.4e-79	61.75	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1DVE7	4.5e-78	59.69	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]

BLAST of CcUC08G148700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 209..229
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 26..263
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 38..51 score: 42.48 coord: 148..162 score: 48.77 coord: 178..198 score: 55.76 coord: 207..224 score: 48.54 coord: 86..101 score: 49.18
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 191..268 e-value: 4.3E-20 score: 73.4
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 36..224 e-value: 1.5E-32 score: 112.8
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 26..263
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 27..190 e-value: 2.2E-43 score: 149.9
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 28..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC08G148700.1	CcUC08G148700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity