CcUC06G123500 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC06G123500
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	Eukaryotic aspartyl protease family protein
Location	CicolChr06: 25969139 .. 25969753 (+)
RNA-Seq Expression	CcUC06G123500
Synteny	CcUC06G123500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCTTGTCCATGTCAATCACAACAAAGCCTTCACGTTTGGCGATGAAGCTGATCCACCGCAACTCGTATCTACATCCACTCTATGACCCCAATGAGACAGTTGAGGGTCGTTGGAAGAGAGAGGAGACGAGCTCAATCGAACGCTTTGCTTATCTTGAGTCGAAGATAAAAGAATTGAAGTCTGTTGGTAGTGAGGCTCGATCAAGTCTCATCCCTTTCAATCGAGGTAGCGGGTTTCTTGTTAATTTGTCGATCGGTTCGCCGCCTGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAGTGTTTGCCTTGTATCAACTGTTTTAGGCAATCGAGCTCATGGTTTGATCCTCTGAAATCAACAAGCTTCAAGATATTGGGCTGTGGGTTTCCTGGCTATAACTACATTAGTGGTTACAAATGCAATGGTTTTAATCAAGCAGAGTATAAATTGAGGTACCTTGGCGGGGATACCTCACAAGGAGTTCTTGCCAAGGAATCACTTCTCTTTGACACATCTGACGAAGGTAGAATTTTTTCGAGCAGCTTTGGGGCTGATTCTAAAACACTTGAAATTACTTTTATCATTTTTTAA

mRNA sequence

ATGGTCTTGTCCATGTCAATCACAACAAAGCCTTCACGTTTGGCGATGAAGCTGATCCACCGCAACTCGTATCTACATCCACTCTATGACCCCAATGAGACAGTTGAGGGTCGTTGGAAGAGAGAGGAGACGAGCTCAATCGAACGCTTTGCTTATCTTGAGTCGAAGATAAAAGAATTGAAGTCTGTTGGTAGTGAGGCTCGATCAAGTCTCATCCCTTTCAATCGAGGTAGCGGGTTTCTTGTTAATTTGTCGATCGGTTCGCCGCCTGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAGTGTTTGCCTTGTATCAACTGTTTTAGGCAATCGAGCTCATGGTTTGATCCTCTGAAATCAACAAGCTTCAAGATATTGGGCTGTGGGTTTCCTGGCTATAACTACATTAGTGGTTACAAATGCAATGGTTTTAATCAAGCAGAGTATAAATTGAGGTACCTTGGCGGGGATACCTCACAAGGAGTTCTTGCCAAGGAATCACTTCTCTTTGACACATCTGACGAAGGTAGAATTTTTTCGAGCAGCTTTGGGGCTGATTCTAAAACACTTGAAATTACTTTTATCATTTTTTAA

Coding sequence (CDS)

ATGGTCTTGTCCATGTCAATCACAACAAAGCCTTCACGTTTGGCGATGAAGCTGATCCACCGCAACTCGTATCTACATCCACTCTATGACCCCAATGAGACAGTTGAGGGTCGTTGGAAGAGAGAGGAGACGAGCTCAATCGAACGCTTTGCTTATCTTGAGTCGAAGATAAAAGAATTGAAGTCTGTTGGTAGTGAGGCTCGATCAAGTCTCATCCCTTTCAATCGAGGTAGCGGGTTTCTTGTTAATTTGTCGATCGGTTCGCCGCCTGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAGTGTTTGCCTTGTATCAACTGTTTTAGGCAATCGAGCTCATGGTTTGATCCTCTGAAATCAACAAGCTTCAAGATATTGGGCTGTGGGTTTCCTGGCTATAACTACATTAGTGGTTACAAATGCAATGGTTTTAATCAAGCAGAGTATAAATTGAGGTACCTTGGCGGGGATACCTCACAAGGAGTTCTTGCCAAGGAATCACTTCTCTTTGACACATCTGACGAAGGTAGAATTTTTTCGAGCAGCTTTGGGGCTGATTCTAAAACACTTGAAATTACTTTTATCATTTTTTAA

Protein sequence

MVLSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKELKSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSSWFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTSDEGRIFSSSFGADSKTLEITFIIF

Homology

[Close]

BLAST of CcUC06G123500 vs. NCBI nr
Match: XP_038878960.1 (aspartic proteinase CDR1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 340.9 bits (873), Expect = 7.7e-90
Identity = 165/185 (89.19%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MVLSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKEL 60
           ++ SM+ TTKPSRLA KLIHRNSYLHPLYDP ET+E R KREETSSIERFAYLESKIKEL
Sbjct: 25  IISSMAFTTKPSRLATKLIHRNSYLHPLYDPTETIEDRSKREETSSIERFAYLESKIKEL 84

Query: 61  KSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSS 120
           KSVG+EARSSLIPFN+GSGFLVNLSIGSPPVTQLVV DTGSSLLWVQCLPCI+CFRQS+S
Sbjct: 85  KSVGNEARSSLIPFNQGSGFLVNLSIGSPPVTQLVVADTGSSLLWVQCLPCIDCFRQSNS 144

Query: 121 WFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTS 180
           WFDPLKSTSFKILGCGF GYNYISGY+CNGFNQAEYKLRYLGGDTSQGVLAKESLLF+T 
Sbjct: 145 WFDPLKSTSFKILGCGFAGYNYISGYRCNGFNQAEYKLRYLGGDTSQGVLAKESLLFETL 204

Query: 181 DEGRI 186
           DEG+I
Sbjct: 205 DEGKI 209

[Close]

BLAST of CcUC06G123500 vs. NCBI nr
Match: XP_038878961.1 (probable aspartic protease At2g35615 isoform X2 [Benincasa hispida])

HSP 1 Score: 339.3 bits (869), Expect = 2.2e-89
Identity = 164/181 (90.61%), Postives = 173/181 (95.58%), Query Frame = 0


Query: 5   MSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKELKSVG 64
           M+ TTKPSRLA KLIHRNSYLHPLYDP ET+E R KREETSSIERFAYLESKIKELKSVG
Sbjct: 1   MAFTTKPSRLATKLIHRNSYLHPLYDPTETIEDRSKREETSSIERFAYLESKIKELKSVG 60

Query: 65  SEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSSWFDP 124
           +EARSSLIPFN+GSGFLVNLSIGSPPVTQLVV DTGSSLLWVQCLPCI+CFRQS+SWFDP
Sbjct: 61  NEARSSLIPFNQGSGFLVNLSIGSPPVTQLVVADTGSSLLWVQCLPCIDCFRQSNSWFDP 120

Query: 125 LKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTSDEGR 184
           LKSTSFKILGCGF GYNYISGY+CNGFNQAEYKLRYLGGDTSQGVLAKESLLF+T DEG+
Sbjct: 121 LKSTSFKILGCGFAGYNYISGYRCNGFNQAEYKLRYLGGDTSQGVLAKESLLFETLDEGK 180

Query: 185 I 186
           I
Sbjct: 181 I 181

[Close]

BLAST of CcUC06G123500 vs. NCBI nr
Match: KAG6589695.1 (Glycosyltransferase BC10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 337.8 bits (865), Expect = 6.5e-89
Identity = 162/185 (87.57%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MVLSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKEL 60
           ++ SM   TKPSRLA +LIHRNSYLHPLYDPNETVE R KREETSSIERFAYLESKIKEL
Sbjct: 584 IISSMMTMTKPSRLATRLIHRNSYLHPLYDPNETVEDRSKREETSSIERFAYLESKIKEL 643

Query: 61  KSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSS 120
           KSVG+ ARS+L PFNRGSGFLVNLSIGSPPV QLVV+DTGSSLLWVQCLPCINCFRQSSS
Sbjct: 644 KSVGNVARSNLSPFNRGSGFLVNLSIGSPPVAQLVVIDTGSSLLWVQCLPCINCFRQSSS 703

Query: 121 WFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTS 180
           WFDPLKS+SFKILGCGFPGYNY+SGY+CNG+NQAEYKLRYLGGDTSQG+LAKESLLF+TS
Sbjct: 704 WFDPLKSSSFKILGCGFPGYNYVSGYRCNGYNQAEYKLRYLGGDTSQGLLAKESLLFETS 763

Query: 181 DEGRI 186
           DEG+I
Sbjct: 764 DEGKI 768

[Close]

BLAST of CcUC06G123500 vs. NCBI nr
Match: XP_023515827.1 (aspartic proteinase CDR1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337.8 bits (865), Expect = 6.5e-89
Identity = 162/185 (87.57%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MVLSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKEL 60
           ++ SM   TKPSRLA +LIHRNSYLHPLYDPNETVE R KREETSSIERFAYLESKIKEL
Sbjct: 44  IISSMMTMTKPSRLATRLIHRNSYLHPLYDPNETVEDRSKREETSSIERFAYLESKIKEL 103

Query: 61  KSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSS 120
           KSVG+ ARS+L PFNRGSGFLVNLSIGSPPV QLVV+DTGSSLLWVQCLPCINCFRQSSS
Sbjct: 104 KSVGNVARSNLSPFNRGSGFLVNLSIGSPPVAQLVVIDTGSSLLWVQCLPCINCFRQSSS 163

Query: 121 WFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTS 180
           WFDPLKS+SFKILGCGFPGYNY+SGY+CNG+NQAEYKLRYLGGDTSQG+LAKESLLF+TS
Sbjct: 164 WFDPLKSSSFKILGCGFPGYNYVSGYRCNGYNQAEYKLRYLGGDTSQGLLAKESLLFETS 223

Query: 181 DEGRI 186
           DEG+I
Sbjct: 224 DEGKI 228

[Close]

BLAST of CcUC06G123500 vs. NCBI nr
Match: KAG7023375.1 (Aspartic proteinase nepenthesin-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.8 bits (865), Expect = 6.5e-89
Identity = 162/185 (87.57%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MVLSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKEL 60
           ++ SM   TKPSRLA +LIHRNSYLHPLYDPNETVE R KREETSSIERFAYLESKIKEL
Sbjct: 155 IISSMMTMTKPSRLATRLIHRNSYLHPLYDPNETVEDRSKREETSSIERFAYLESKIKEL 214

Query: 61  KSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSS 120
           KSVG+ ARS+L PFNRGSGFLVNLSIGSPPV QLVV+DTGSSLLWVQCLPCINCFRQSSS
Sbjct: 215 KSVGNVARSNLSPFNRGSGFLVNLSIGSPPVAQLVVIDTGSSLLWVQCLPCINCFRQSSS 274

Query: 121 WFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTS 180
           WFDPLKS+SFKILGCGFPGYNY+SGY+CNG+NQAEYKLRYLGGDTSQG+LAKESLLF+TS
Sbjct: 275 WFDPLKSSSFKILGCGFPGYNYVSGYRCNGYNQAEYKLRYLGGDTSQGLLAKESLLFETS 334

Query: 181 DEGRI 186
           DEG+I
Sbjct: 335 DEGKI 339

[Close]

BLAST of CcUC06G123500 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 52/181 (28.73%), Postives = 94/181 (51.93%), Query Frame = 0


Query: 3   LSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKELKS 62
           +++S +  P   +++LIHR+S L P+Y+P  TV  R       S+ R      ++ +   
Sbjct: 15  VTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRFNHQLSQ--- 74

Query: 63  VGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSSWF 122
             ++ +S LI       F ++++IG+PP+    + DTGS L WVQC PC  C++++   F
Sbjct: 75  --TDLQSGLI--GADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPIF 134

Query: 123 DPLKSTSFKILGCGFPGYNYISGYK--CNGFNQ-AEYKLRYLGGDTSQGVLAKESLLFDT 181
           D  KS+++K   C       +S  +  C+  N   +Y+  Y     S+G +A E++  D+
Sbjct: 135 DKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDS 188

[Close]

BLAST of CcUC06G123500 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.5e-16
Identity = 53/175 (30.29%), Postives = 90/175 (51.43%), Query Frame = 0


Query: 12  SRLAMKLIHRNSYLHPLY-DPNETVEGRWKREETSSIERFAYLESKI-------KELKSV 71
           S+  ++L+HR+ +    Y + +  +  R +R+          +  K+        E+   
Sbjct: 57  SKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDF 116

Query: 72  GSEARSSLIPFNRGSG-FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSSWF 131
           GS+  S +   ++GSG + V + +GSPP  Q +V+D+GS ++WVQC PC  C++QS   F
Sbjct: 117 GSDIVSGM---DQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVF 176

Query: 132 DPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLF 178
           DP KS S+  + CG    + I    C+      Y++ Y  G  ++G LA E+L F
Sbjct: 177 DPAKSGSYTGVSCGSSVCDRIENSGCHS-GGCRYEVMYGDGSYTKGTLALETLTF 227

[Close]

BLAST of CcUC06G123500 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 9.9e-16
Identity = 53/167 (31.74%), Postives = 78/167 (46.71%), Query Frame = 0


Query: 18  LIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKELKSVGSEARSSLIPFNRG 77
           LIHR+S   P Y+P ET   R +     S+ R  +   K               I     
Sbjct: 35  LIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHFTEK--------DNTPQPQIDLTSN 94

Query: 78  SG-FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSSWFDPLKSTSFKILGCG 137
           SG +L+N+SIG+PP   + + DTGS LLW QC PC +C+ Q    FDP  S+++K + C 
Sbjct: 95  SGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCS 154

Query: 138 FPGYNYISGY-KCN-GFNQAEYKLRYLGGDTSQGVLAKESLLFDTSD 182
                 +     C+   N   Y L Y     ++G +A ++L   +SD
Sbjct: 155 SSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSD 193

[Close]

BLAST of CcUC06G123500 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.9e-15
Identity = 43/128 (33.59%), Postives = 69/128 (53.91%), Query Frame = 0


Query: 54  ESKIKELKSVGSEARSSLIPFNRGSG-FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCI 113
           E +++ + ++   +     P   G G +L+N++IG+P  +   ++DTGS L+W QC PC 
Sbjct: 69  ERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCT 128

Query: 114 NCFRQSSSWFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAK 173
            CF Q +  F+P  S+SF  L C       +    CN  N+ +Y   Y  G T+QG +A 
Sbjct: 129 QCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNN-NECQYTYGYGDGSTTQGYMAT 188

Query: 174 ESLLFDTS 181
           E+  F+TS
Sbjct: 189 ETFTFETS 195

[Close]

BLAST of CcUC06G123500 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.1e-13
Identity = 39/98 (39.80%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 80  FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSSWFDPLKSTSFKILGCGFPG 139
           +L+NLSIG+P      ++DTGS L+W QC PC  CF QS+  F+P  S+SF  L C    
Sbjct: 95  YLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQL 154

Query: 140 YNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLF 178
              +S   C+  N  +Y   Y  G  +QG +  E+L F
Sbjct: 155 CQALSSPTCSN-NFCQYTYGYGDGSETQGSMGTETLTF 191

[Close]

BLAST of CcUC06G123500 vs. ExPASy TrEMBL
Match: A0A1S3BY28 (aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103494349 PE=3 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.2e-88
Identity = 164/186 (88.17%), Postives = 176/186 (94.62%), Query Frame = 0


Query: 1   MVLSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKEL 60
           ++LS SITTKPSRLA KLIHRNSYLHPLYDPNETVE R KRE+ SSIERFA+LESKIKEL
Sbjct: 38  IILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASSIERFAFLESKIKEL 97

Query: 61  KSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSS 120
           KSVG+EARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCF+QS+S
Sbjct: 98  KSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSTS 157

Query: 121 WFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTS 180
           WFDPLKS SFK LGCGFPGYNYI+GYKCNG NQAEYKLRYLGGD+SQG+LAKESLLF+T 
Sbjct: 158 WFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYKLRYLGGDSSQGILAKESLLFETL 217

Query: 181 DEGRIF 187
           DEG +F
Sbjct: 218 DEGGVF 222

[Close]

BLAST of CcUC06G123500 vs. ExPASy TrEMBL
Match: A0A5A7UU11 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005510 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.6e-88
Identity = 164/185 (88.65%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MVLSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKEL 60
           ++LS SITTKPSRLA KLIHRNSYLHPLYDPNETVE R KRE+ SSIERFA+LESKIKEL
Sbjct: 414 IILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASSIERFAFLESKIKEL 473

Query: 61  KSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSS 120
           KSVG+EARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCF+QS+S
Sbjct: 474 KSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSTS 533

Query: 121 WFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTS 180
           WFDPLKS SFK LGCGFPGYNYI+GYKCNG NQAEYKLRYLGGD+SQG+LAKESLLF+T 
Sbjct: 534 WFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYKLRYLGGDSSQGILAKESLLFETL 593

Query: 181 DEGRI 186
           DEG+I
Sbjct: 594 DEGKI 597

[Close]

BLAST of CcUC06G123500 vs. ExPASy TrEMBL
Match: A0A6J1E195 (probable aspartic protease At2g35615 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429882 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 2.0e-88
Identity = 161/185 (87.03%), Postives = 174/185 (94.05%), Query Frame = 0


Query: 1   MVLSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKEL 60
           ++ SM   TKPSRLA +LIHRNSYLHPLYDPNETVE R KREETSSIERFAYLESKIKEL
Sbjct: 44  IISSMMTMTKPSRLATRLIHRNSYLHPLYDPNETVEDRSKREETSSIERFAYLESKIKEL 103

Query: 61  KSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSS 120
           KS+G+ ARS+L PFNRGSGFLVNLSIGSPPV QLVV+DTGSSLLWVQCLPCINCFRQSSS
Sbjct: 104 KSIGNVARSNLSPFNRGSGFLVNLSIGSPPVAQLVVIDTGSSLLWVQCLPCINCFRQSSS 163

Query: 121 WFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTS 180
           WFDPLKS+SFKILGCGF GYNY+SGYKCNG+NQAEYKLRYLGGDTSQG+LAKESLLF+TS
Sbjct: 164 WFDPLKSSSFKILGCGFRGYNYVSGYKCNGYNQAEYKLRYLGGDTSQGLLAKESLLFETS 223

Query: 181 DEGRI 186
           DEG+I
Sbjct: 224 DEGKI 228

[Close]

BLAST of CcUC06G123500 vs. ExPASy TrEMBL
Match: A0A6J1E1B4 (probable aspartic protease At2g35615 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429882 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 5.9e-88
Identity = 160/181 (88.40%), Postives = 171/181 (94.48%), Query Frame = 0


Query: 5   MSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKELKSVG 64
           M   TKPSRLA +LIHRNSYLHPLYDPNETVE R KREETSSIERFAYLESKIKELKS+G
Sbjct: 1   MMTMTKPSRLATRLIHRNSYLHPLYDPNETVEDRSKREETSSIERFAYLESKIKELKSIG 60

Query: 65  SEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSSWFDP 124
           + ARS+L PFNRGSGFLVNLSIGSPPV QLVV+DTGSSLLWVQCLPCINCFRQSSSWFDP
Sbjct: 61  NVARSNLSPFNRGSGFLVNLSIGSPPVAQLVVIDTGSSLLWVQCLPCINCFRQSSSWFDP 120

Query: 125 LKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTSDEGR 184
           LKS+SFKILGCGF GYNY+SGYKCNG+NQAEYKLRYLGGDTSQG+LAKESLLF+TSDEG+
Sbjct: 121 LKSSSFKILGCGFRGYNYVSGYKCNGYNQAEYKLRYLGGDTSQGLLAKESLLFETSDEGK 180

Query: 185 I 186
           I
Sbjct: 181 I 181

[Close]

BLAST of CcUC06G123500 vs. ExPASy TrEMBL
Match: A0A5D3DZ20 (Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 PE=3 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 7.7e-88
Identity = 163/185 (88.11%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MVLSMSITTKPSRLAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKEL 60
           ++LS SI TKPSRLA KLIHRNSYLHPLYDPNETVE R KRE+ SSIERFA+LESKIKEL
Sbjct: 414 IILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASSIERFAFLESKIKEL 473

Query: 61  KSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSS 120
           KSVG+EARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCF+QS+S
Sbjct: 474 KSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSTS 533

Query: 121 WFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDTS 180
           WFDPLKS SFK LGCGFPGYNYI+GYKCNG NQAEYKLRYLGGD+SQG+LAKESLLF+T 
Sbjct: 534 WFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYKLRYLGGDSSQGILAKESLLFETL 593

Query: 181 DEGRI 186
           DEG+I
Sbjct: 594 DEGKI 597

[Close]

BLAST of CcUC06G123500 vs. TAIR 10
Match: AT2G23945.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 108.2 bits (269), Expect = 7.8e-24
Identity = 73/175 (41.71%), Postives = 93/175 (53.14%), Query Frame = 0


Query: 10  KPSRLAMKLIHRNSY--LHPLYDPNETVEGRWKREETSSIERFAYLESKI-KELKSVGSE 69
           KP+R+AMKLIHR S   L+P      T E   K     S  RF YL++ I KEL S  S 
Sbjct: 25  KPNRMAMKLIHRESVARLNPNARVPITPEDHIKHLTDISSARFKYLQNSIDKELGS--SN 84

Query: 70  ARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCF--RQSSSWFDP 129
            +  +    + S FLVN S+G PPV QL ++DTGSSLLW+QC PC +C         F+P
Sbjct: 85  FQVDVEQAIKTSLFLVNFSVGQPPVPQLTIMDTGSSLLWIQCQPCKHCSSDHMIHPVFNP 144

Query: 130 LKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGDTSQGVLAKESLLFDT 180
             S++F    C      Y     C   N+  Y+  Y+ G  S+GVLAKE L F T
Sbjct: 145 ALSSTFVECSCDDRFCRYAPNGHCGSSNKCVYEQVYISGTGSKGVLAKERLTFTT 197

[Close]

BLAST of CcUC06G123500 vs. TAIR 10
Match: AT4G30040.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 103.6 bits (257), Expect = 1.9e-22
Identity = 57/135 (42.22%), Postives = 78/135 (57.78%), Query Frame = 0


Query: 45  SSIERFAYLESKIKELKSVGSEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLL 104
           +S+ER  YL++K              +IP      FLVN+SIGSPP+TQL+ +DT S LL
Sbjct: 54  ASVERLEYLKAKTTGDIIAHLSPNVPIIP----QAFLVNISIGSPPITQLLHMDTASDLL 113

Query: 105 WVQCLPCINCFRQSSSWFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEYKLRYLGGD 164
           W+QCLPCINC+ QS   FDP +S + +   C    Y+  S          EY +RY+   
Sbjct: 114 WIQCLPCINCYAQSLPIFDPSRSYTHRNETCRTSQYSMPSLKFNANTRSCEYSMRYVDDT 173

Query: 165 TSQGVLAKESLLFDT 180
            S+G+LA+E LLF+T
Sbjct: 174 GSKGILAREMLLFNT 184

[Close]

BLAST of CcUC06G123500 vs. TAIR 10
Match: AT4G30030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 96.7 bits (239), Expect = 2.3e-20
Identity = 53/149 (35.57%), Postives = 84/149 (56.38%), Query Frame = 0


Query: 38  RWKREETSSIERFAYLESKIKELKSVGS-EARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 97
           R K +E+S I +  YL SK      + +    S + P    + FL N+SIG+PPV QL++
Sbjct: 36  RTKTQESSKI-KIGYLHSKSTPASRLDNLWTVSHVTPIPNPAAFLANISIGNPPVPQLLL 95

Query: 98  VDTGSSLLWVQCLPCINCFRQSSSWFDPLKSTSFKILGCGFPGYNYISGYKCNGFNQAEY 157
           +DTGS L W+ CLPC  C+ Q+  +F P +S++++   C    +     ++       +Y
Sbjct: 96  IDTGSDLTWIHCLPC-KCYPQTIPFFHPSRSSTYRNASCVSAPHAMPQIFRDEKTGNCQY 155

Query: 158 KLRYLGGDTSQGVLAKESLLFDTSDEGRI 186
            LRY     ++G+LA+E L F+TSD+G I
Sbjct: 156 HLRYRDFSNTRGILAEEKLTFETSDDGLI 182

[Close]

BLAST of CcUC06G123500 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 92.0 bits (227), Expect = 5.8e-19
Identity = 59/171 (34.50%), Postives = 93/171 (54.39%), Query Frame = 0


Query: 14  LAMKLIHRNSYLHPLYDPNETVEGRWKREETSSIERFAYLESKIKELKSVGSEARSSLIP 73
           L ++LIHR+S   PLY+P+ TV  R       SI R     +K        ++ +S LI 
Sbjct: 29  LTVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISRSRRFTTK--------TDLQSGLI- 88

Query: 74  FNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSSWFDPLKSTSFKIL 133
            + G  + +++SIG+PP     + DTGS L WVQC PC  C++Q+S  FD  KS+++K  
Sbjct: 89  -SNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPCQQCYKQNSPLFDKKKSSTYKTE 148

Query: 134 GCGFPGYNYISGYKCNGFNQAE--YKLRYLGGDTS--QGVLAKESLLFDTS 181
            C       +S ++  G ++++   K RY  GD S  +G +A E++  D+S
Sbjct: 149 SCDSKTCQALSEHE-EGCDESKDICKYRYSYGDNSFTKGDVATETISIDSS 188

[Close]

BLAST of CcUC06G123500 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 88.2 bits (217), Expect = 8.3e-18
Identity = 51/121 (42.15%), Postives = 67/121 (55.37%), Query Frame = 0


Query: 73  PFNRGSG-FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFRQSSSWFDPLKSTSFK 132
           P + GSG FL+ LSIG+P V    +VDTGS L+W QC PC  CF Q +  FDP KS+S+ 
Sbjct: 99  PTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYS 158

Query: 133 ILGCGFPGYNYISGYKCNGFNQA-EYKLRYLGGDTSQGVLAKESLLFDTSDEGRIFSSSF 192
            +GC     N +    CN    A EY   Y    +++G+LA E+  F+  DE  I    F
Sbjct: 159 KVGCSSGLCNALPRSNCNEDKDACEYLYTYGDYSSTRGLLATETFTFE--DENSISGIGF 217

The following BLAST results are available for this feature:

BLAST of CcUC06G123500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878960.1	7.7e-90	89.19	aspartic proteinase CDR1-like isoform X1 [Benincasa hispida]	[more]
XP_038878961.1	2.2e-89	90.61	probable aspartic protease At2g35615 isoform X2 [Benincasa hispida]	[more]
KAG6589695.1	6.5e-89	87.57	Glycosyltransferase BC10, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023515827.1	6.5e-89	87.57	aspartic proteinase CDR1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG7023375.1	6.5e-89	87.57	Aspartic proteinase nepenthesin-2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CcUC06G123500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3EBM5	1.5e-16	28.73	Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561...	[more]
Q9LHE3	4.5e-16	30.29	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q6XBF8	9.9e-16	31.74	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q766C2	2.9e-15	33.59	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	2.1e-13	39.80	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of CcUC06G123500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BY28	1.2e-88	88.17	aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103494349 PE=3 SV=1	[more]
A0A5A7UU11	1.6e-88	88.65	Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]
A0A6J1E195	2.0e-88	87.03	probable aspartic protease At2g35615 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1E1B4	5.9e-88	88.40	probable aspartic protease At2g35615 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A5D3DZ20	7.7e-88	88.11	Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 ...	[more]

BLAST of CcUC06G123500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23945.1	7.8e-24	41.71	Eukaryotic aspartyl protease family protein	[more]
AT4G30040.1	1.9e-22	42.22	Eukaryotic aspartyl protease family protein	[more]
AT4G30030.1	2.3e-20	35.57	Eukaryotic aspartyl protease family protein	[more]
AT1G31450.1	5.8e-19	34.50	Eukaryotic aspartyl protease family protein	[more]
AT2G03200.1	8.3e-18	42.15	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 61..201 e-value: 5.5E-24 score: 87.1
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 78..185
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 80..192 e-value: 1.7E-21 score: 77.2
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 7..184
None	No IPR available	PANTHER	PTHR47967:SF14	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 7..184
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 95..106
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 80..204 score: 19.250101
IPR034164	Pepsin-like domain	CDD	cd05471	pepsin_like	coord: 80..146 e-value: 1.35201E-16 score: 73.9988

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC06G123500.1	CcUC06G123500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0016757	glycosyltransferase activity