CcUC05G087860 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC05G087860
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	CASP-like protein
Location	CicolChr05: 5594569 .. 5596915 (+)
RNA-Seq Expression	CcUC05G087860
Synteny	CcUC05G087860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTATTATTACATAGAGAAAGAGAAGTTAGGGTTTTTGTTTTTGGGTTTTTTTTAATGCTATTTTTGTTGGGAGAAAAATGGAGGTGGAGAGAAGAAGAGTGAAAATGGAAGATGGGAAAAATGAAGAGATGAAGAAAATGGGAAATTATGATAATAATAATAAGGTTGAAATGGTTTTTAGGGTTTTGGGATTTGTGTTGAGTTTTGTGGCAGCCATTGTTGTGGGACTCAACAAACAAACCAAGGTTGTTCCTCTTACTGTCTCCCTCAACCTTCATCCTTTGGATTATACTTTCACTGCTAAATGGCATTACTTATCTGCTTTTGTGTAAGTCTCTTTCTCTCTCTCTCCCTCACTAAATTCATATCATCTTTTAACATATCTTCGTTTGTTTTTTCTTTTCAGTTTAATTACATGTAGGTACATATTCGAATCTTTAACATTTTGATTTTGATTGTGAGTTTCAACTAATTTTTTTGGTGTGAAAAATTAAATATTGGTTTCTTTTTTGTTAGTAAATGTGACGTAAAAGTGATATTGGTGGTAATTTGATGATTGTGAAGTCACTTTTTTTAGAGAATGAATCAATGTTATAACTTTCAAAAGTATTCATCCAAAATCGTTTATGAGTATTATTATTTTACAAAAATTACTTATAAATTAATTATTATCGATATATATGTGTGTGTTCAGTAGAACATATCTTAGATATAATTTTCTAAATTATATTGATTTTCTTTTGACAAAATGAAGTTTTTGTATGAATGTGCATTTGTGTCCAAGATTAGTGAACAATAAATGGGCTTTTATCATATGTTTTACCCTTTTCCCAATTCAACGTCAACAATTTCAAATAATATGGCGACAATATTTCCCAACTGAATGATAAGGATGTTCATTTTTTTTTTTTTTTTTTAATTATATTTTCCCCGATTTGAAAATTTTGATATACAATTAAAAATATAATATTTAATTAAGAACCATTAATAAAGGAAGTATATCATCTACCACAAATCAAAGGTTCAAATATCTGCTCTTACGTGTTCATGTTGAATAACTTTTAAGAAAAGTCTTTATAAATGTACACTAATTAATTTTTGTATTTATTTCAAAACATTATGGTTGTTATATAGAGTTTATGCTTAAAATATTATGTTTGTATATGTATTTTGGAGTTGGTTCAATTTTACTATTTTTAAATATGTTCAACTTTATTCTTTGTACTTTTAATAAATCTTAAATTTTGTTCTTCCAAGTTAATCTATATTAAATTTTTTTAAATAATAATAATAAATCTTGAATTTAATTCCTCTCCGTTAATCTATATTAAAATTGGTCAAATAATAATAATTATTATTATAATGTAAGAAAATAGATGAATATGTTTTTAAAATGTACAGTGAAAATGCTAATATATAAAAGGTTTTTGAAAAATTAATAACAAAATTGAGTTTAAGATTTATTGAAAGTAGCAGCACTAAAATTAGGCATTTGAAAATATAGCAACTAAGATCTAAGAAGTGTTCTAAATAGTATTTTATAACTTTTTTTTTAGATGCAACGATATGATCCTTAATGTAAATTATCTAATTAAGTAGCCACTACAAACCTAACATAGCATCATGTGACGTGTGAAAGCTATTACGGCTCTAACACTGACAAAATAAATTTAACCTAAGTTTTAAGACCATATTTCAGTAACATCTAAAAGCATATTGTGTTATTTTTAGGTACCTTTTAGCAATAAATATAATAGCATCTTCATATAGCTTCGTATCATTGTTCCTTTTGCTGAAAAACAAGAGCAAGGACAACATCTTAGGCTTATTGATCATCGTTTTCGACACGGTCATGGCGGCTCTGCTCTTCTCCAGCAGCGGAGCTGCAGCTGCGGTCGGGGTCATTGCCTACCACGGGAACTCACACGTGCAATGGAACAAAGTGTGCGATATATATGGCAGATTTTGCAAGCAAGTAGCAGCCTCTACTGTGCTTTCTCTTGCTGGAGCTGTAGTATTTATGTCGCTAGTTGTGTTGGCTTCTGTGGGCCTTCAAAAGAGGCCCAATTAAATGGGCTGGGCTTTGGGCCTGTTTTTAGGCTTATTCACAGTCCATCAAAATGGGCTTGGGCTCAGTTTCTTCTAAAATCGTCACAGCCCATTTAAAGCTAATTAGGTTATCAGTTGTGGAATTGAAATGTTATTTGTAATACTTGTAATAGCCAAAGGAGACTGTTTACGTAGAATATTTTATTTACCACTCCCACCCCTCTCTTAGAAAAGTACAATATTTATTAACGTCTTCACGATTAGGATATCTACTCATTTTTTTTGGTTGAAATTGGAG

mRNA sequence

TTATTATTACATAGAGAAAGAGAAGTTAGGGTTTTTGTTTTTGGGTTTTTTTTAATGCTATTTTTGTTGGGAGAAAAATGGAGGTGGAGAGAAGAAGAGTGAAAATGGAAGATGGGAAAAATGAAGAGATGAAGAAAATGGGAAATTATGATAATAATAATAAGGTTGAAATGGTTTTTAGGGTTTTGGGATTTGTGTTGAGTTTTGTGGCAGCCATTGTTGTGGGACTCAACAAACAAACCAAGGTTGTTCCTCTTACTGTCTCCCTCAACCTTCATCCTTTGGATTATACTTTCACTGCTAAATGGCATTACTTATCTGCTTTTGTGTACCTTTTAGCAATAAATATAATAGCATCTTCATATAGCTTCGTATCATTGTTCCTTTTGCTGAAAAACAAGAGCAAGGACAACATCTTAGGCTTATTGATCATCGTTTTCGACACGGTCATGGCGGCTCTGCTCTTCTCCAGCAGCGGAGCTGCAGCTGCGGTCGGGGTCATTGCCTACCACGGGAACTCACACGTGCAATGGAACAAAGTGTGCGATATATATGGCAGATTTTGCAAGCAAGTAGCAGCCTCTACTGTGCTTTCTCTTGCTGGAGCTGTAGTATTTATGTCGCTAGTTGTGTTGGCTTCTGTGGGCCTTCAAAAGAGGCCCAATTAAATGGGCTGGGCTTTGGGCCTGTTTTTAGGCTTATTCACAGTCCATCAAAATGGGCTTGGGCTCAGTTTCTTCTAAAATCGTCACAGCCCATTTAAAGCTAATTAGGTTATCAGTTGTGGAATTGAAATGTTATTTGTAATACTTGTAATAGCCAAAGGAGACTGTTTACGTAGAATATTTTATTTACCACTCCCACCCCTCTCTTAGAAAAGTACAATATTTATTAACGTCTTCACGATTAGGATATCTACTCATTTTTTTTGGTTGAAATTGGAG

Coding sequence (CDS)

ATGGAGGTGGAGAGAAGAAGAGTGAAAATGGAAGATGGGAAAAATGAAGAGATGAAGAAAATGGGAAATTATGATAATAATAATAAGGTTGAAATGGTTTTTAGGGTTTTGGGATTTGTGTTGAGTTTTGTGGCAGCCATTGTTGTGGGACTCAACAAACAAACCAAGGTTGTTCCTCTTACTGTCTCCCTCAACCTTCATCCTTTGGATTATACTTTCACTGCTAAATGGCATTACTTATCTGCTTTTGTGTACCTTTTAGCAATAAATATAATAGCATCTTCATATAGCTTCGTATCATTGTTCCTTTTGCTGAAAAACAAGAGCAAGGACAACATCTTAGGCTTATTGATCATCGTTTTCGACACGGTCATGGCGGCTCTGCTCTTCTCCAGCAGCGGAGCTGCAGCTGCGGTCGGGGTCATTGCCTACCACGGGAACTCACACGTGCAATGGAACAAAGTGTGCGATATATATGGCAGATTTTGCAAGCAAGTAGCAGCCTCTACTGTGCTTTCTCTTGCTGGAGCTGTAGTATTTATGTCGCTAGTTGTGTTGGCTTCTGTGGGCCTTCAAAAGAGGCCCAATTAA

Protein sequence

MEVERRRVKMEDGKNEEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLIIVFDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVLASVGLQKRPN

Homology

[Close]

BLAST of CcUC05G087860 vs. NCBI nr
Match: XP_038879800.1 (CASP-like protein 1E2 [Benincasa hispida])

HSP 1 Score: 322.0 bits (824), Expect = 3.5e-84
Identity = 179/197 (90.86%), Postives = 186/197 (94.42%), Query Frame = 0


Query: 1   MEVERRRVKME-DGKNEEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVP 60
           MEVERRRVKME + K++EMKK+GNY  +NKVEMVFRVLGFVLSFVAAIVVGLNKQTK+VP
Sbjct: 1   MEVERRRVKMEAERKDQEMKKIGNY--HNKVEMVFRVLGFVLSFVAAIVVGLNKQTKLVP 60

Query: 61  LTVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLII 120
           LTVSLNL PLDYTF AKWHYLSAFVYLLA NIIA SYSF+SLFLLLKNKSKDNILGLLII
Sbjct: 61  LTVSLNLPPLDYTFIAKWHYLSAFVYLLATNIIACSYSFISLFLLLKNKSKDNILGLLII 120

Query: 121 VFDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVV 180
           V DTVM ALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVV
Sbjct: 121 VLDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVV 180

Query: 181 FMSLVVLASVGLQKRPN 197
           FM LVVLASVGLQKRPN
Sbjct: 181 FMLLVVLASVGLQKRPN 195

[Close]

BLAST of CcUC05G087860 vs. NCBI nr
Match: XP_004134188.2 (CASP-like protein 1E2 [Cucumis sativus] >KGN57071.1 hypothetical protein Csa_010101 [Cucumis sativus])

HSP 1 Score: 315.1 bits (806), Expect = 4.3e-82
Identity = 177/198 (89.39%), Postives = 181/198 (91.41%), Query Frame = 0


Query: 1   MEVERRRVKM-EDGKNEEMKKMGN-YDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVV 60
           MEVERRR KM E+ KNEEMKKMGN YDN +KVEMVFRVLGFVLSFVAAIVVGLN QTKVV
Sbjct: 1   MEVERRRGKMEEERKNEEMKKMGNDYDNYHKVEMVFRVLGFVLSFVAAIVVGLNNQTKVV 60

Query: 61  PLTVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLI 120
           PL VSLN  PLDYTF AKWHYLSAFVYLLA NIIA SYSF+SLFLLLKNKSKDNILGLLI
Sbjct: 61  PLAVSLNSPPLDYTFIAKWHYLSAFVYLLATNIIACSYSFLSLFLLLKNKSKDNILGLLI 120

Query: 121 IVFDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAV 180
           IV DTVM ALLFS SGAA AVGVIAY GN+HVQWNKVCDIYGRFCKQVAASTVLSLAGAV
Sbjct: 121 IVLDTVMVALLFSGSGAAGAVGVIAYQGNTHVQWNKVCDIYGRFCKQVAASTVLSLAGAV 180

Query: 181 VFMSLVVLASVGLQKRPN 197
           VFMSLVVLASVGLQKRPN
Sbjct: 181 VFMSLVVLASVGLQKRPN 198

[Close]

BLAST of CcUC05G087860 vs. NCBI nr
Match: XP_008438805.1 (PREDICTED: CASP-like protein 1E2 [Cucumis melo])

HSP 1 Score: 306.6 bits (784), Expect = 1.5e-79
Identity = 176/201 (87.56%), Postives = 180/201 (89.55%), Query Frame = 0


Query: 1   MEVERRRVKM-EDGKNEEMK--KMGNYDNN--NKVEMVFRVLGFVLSFVAAIVVGLNKQT 60
           +E ERRR KM E+ KNEEMK  KMGNY +N  +KVEMVFRVLGFVLSFVAAIVVGLN QT
Sbjct: 3   VETERRRGKMEEERKNEEMKKIKMGNYYDNYYHKVEMVFRVLGFVLSFVAAIVVGLNNQT 62

Query: 61  KVVPLTVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILG 120
           KVVPLTVSLN  PLDYTF AKWHYLSAFVYLLA NIIA SYSFVSLFLLLK KSKDNILG
Sbjct: 63  KVVPLTVSLNTPPLDYTFIAKWHYLSAFVYLLATNIIACSYSFVSLFLLLKKKSKDNILG 122

Query: 121 LLIIVFDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLA 180
           LLIIV DTVM ALLFS SGAA AVGVIAY GNSHVQWNKVCDIYGRFCKQVAASTVLSLA
Sbjct: 123 LLIIVLDTVMVALLFSGSGAAGAVGVIAYQGNSHVQWNKVCDIYGRFCKQVAASTVLSLA 182

Query: 181 GAVVFMSLVVLASVGLQKRPN 197
           GAVVFMSLVVLASVGLQKRPN
Sbjct: 183 GAVVFMSLVVLASVGLQKRPN 203

[Close]

BLAST of CcUC05G087860 vs. NCBI nr
Match: XP_022975463.1 (CASP-like protein 1E2 [Cucurbita maxima])

HSP 1 Score: 277.3 bits (708), Expect = 1.0e-70
Identity = 153/195 (78.46%), Postives = 166/195 (85.13%), Query Frame = 0


Query: 1   MEVERRRVKMEDGKNEEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPL 60
           MEVERR VK  + + E+M K  N   N+  +M FRVLGFVLS VAAIVVGLNKQ+KVVPL
Sbjct: 1   MEVERRSVKKGEEEEEDMNKNKNVMVNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPL 60

Query: 61  TVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLIIV 120
           TVSLNL PLDYT  A WHYLSA VYLL+ N+IA SYSF+S   LLKNK+KDNIL LL+IV
Sbjct: 61  TVSLNLPPLDYTLIANWHYLSALVYLLSTNVIACSYSFLSFAFLLKNKNKDNILALLVIV 120

Query: 121 FDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVF 180
            DTVM ALLFSSSGAAAAVGVIAYHGNSHVQWNKVCD+YGRFCKQ+AASTVLSLAGAVVF
Sbjct: 121 LDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVF 180

Query: 181 MSLVVLASVGLQKRP 196
           M LVVLASVGLQKRP
Sbjct: 181 MLLVVLASVGLQKRP 195

[Close]

BLAST of CcUC05G087860 vs. NCBI nr
Match: KAG7028955.1 (hypothetical protein SDJN02_10138 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 274.6 bits (701), Expect = 6.5e-70
Identity = 155/195 (79.49%), Postives = 167/195 (85.64%), Query Frame = 0


Query: 1   MEVERRRVKMEDGKNEEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPL 60
           MEVERR V+ E+   E+M K  N   N+  +M FRVLGFVLS VAAIVVGLNKQ+KVVPL
Sbjct: 1   MEVERRSVEKEE--EEDMNK--NVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPL 60

Query: 61  TVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLIIV 120
           TVSLNL PLDYT TA WHYLSA VYLLA N+IA SYSF+S   LLKNK+KDNIL LL+IV
Sbjct: 61  TVSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIV 120

Query: 121 FDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVF 180
            DTVM ALLFSSSGAAAAVGVIAYHGNSHVQWNKVCD+YGRFCKQ+AASTVLSLAGAVVF
Sbjct: 121 LDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVF 180

Query: 181 MSLVVLASVGLQKRP 196
           M LVVLASVGLQKRP
Sbjct: 181 MLLVVLASVGLQKRP 191

[Close]

BLAST of CcUC05G087860 vs. ExPASy Swiss-Prot
Match: C6SZP8 (CASP-like protein 1E2 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 2.8e-39
Identity = 86/164 (52.44%), Postives = 117/164 (71.34%), Query Frame = 0


Query: 31  EMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLHPLDYTFTAKWHYLSAFVYLLAIN 90
           +++ R+L F ++ VAAIV+ ++KQTKVVP+ +S +L PLD   TAKWH +SA VY L  N
Sbjct: 23  DLLLRLLAFTVTLVAAIVIAVDKQTKVVPIQLSDSLPPLDVPLTAKWHQMSAIVYFLVTN 82

Query: 91  IIASSYSFVSLFLLLKNKSKDNILGLLIIVFDTVMAALLFSSSGAAAAVGVIAYHGNSHV 150
            IA +Y+ +SL L L N+ K   L  LI V D  M ALLFS +GAAAAVGV+ Y GNSHV
Sbjct: 83  AIACTYAVLSLLLALVNRGKSKGLWTLIAVLDAFMVALLFSGNGAAAAVGVLGYKGNSHV 142

Query: 151 QWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVLASVGLQKR 195
            WNKVC+++G+FC Q+AAS  +SL G++ F+ LV++  V L +R
Sbjct: 143 NWNKVCNVFGKFCDQMAASIGVSLIGSLAFLLLVIIPGVRLHRR 186

[Close]

BLAST of CcUC05G087860 vs. ExPASy Swiss-Prot
Match: C6TBD0 (CASP-like protein 1E1 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.0e-39
Identity = 85/164 (51.83%), Postives = 118/164 (71.95%), Query Frame = 0


Query: 31  EMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLHPLDYTFTAKWHYLSAFVYLLAIN 90
           +++ R+L F ++ VAAIV+ ++KQTK+VP+ +S +  PL+   TAKWH +SAFVY L  N
Sbjct: 24  DLLLRLLAFTVTLVAAIVIAVDKQTKLVPIQLSDSFPPLNVPLTAKWHQMSAFVYFLVTN 83

Query: 91  IIASSYSFVSLFLLLKNKSKDNILGLLIIVFDTVMAALLFSSSGAAAAVGVIAYHGNSHV 150
            IA +Y+ +SL L L N+ K   L  LI V DT M ALLFS +GAAAAVG++ Y GNSHV
Sbjct: 84  AIACTYAAMSLLLALVNRGKSKGLWTLIAVLDTFMVALLFSGNGAAAAVGILGYKGNSHV 143

Query: 151 QWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVLASVGLQKR 195
            WNKVC+++G+FC Q+AAS  +SL G++ F+ LVV+  V L +R
Sbjct: 144 NWNKVCNVFGKFCDQMAASIGVSLIGSLAFLLLVVIPVVRLHRR 187

[Close]

BLAST of CcUC05G087860 vs. ExPASy Swiss-Prot
Match: A7NW78 (CASP-like protein 1E2 OS=Vitis vinifera OX=29760 GN=VIT_05s0020g01820 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.7e-36
Identity = 83/164 (50.61%), Postives = 112/164 (68.29%), Query Frame = 0


Query: 31  EMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLHPLDYTFTAKWHYLSAFVYLLAIN 90
           ++V RV+   L+ VAA+++G++KQTKVV L +   L P+D   TAKW YLSAFVY +  N
Sbjct: 31  DLVLRVVALALTLVAAVLLGVDKQTKVVSLQLLPTLPPMDVPVTAKWRYLSAFVYFVVSN 90

Query: 91  IIASSYSFVSLFLLLKNKSKDNILGLLIIVFDTVMAALLFSSSGAAAAVGVIAYHGNSHV 150
            IA SY+ +SL L + N   +  LGL I V D VM ALLFSS+GAA A+G++ Y GNS V
Sbjct: 91  AIACSYAALSLLLSVGNSKGNKGLGLAITVMDLVMVALLFSSNGAAGAIGLMGYEGNSRV 150

Query: 151 QWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVLASVGLQKR 195
           +W KVC+++G+FC QVA +  LS  G + F  LVV+A+  L KR
Sbjct: 151 RWGKVCNVFGKFCNQVAVALGLSFFGGLAFFLLVVMAAFALNKR 194

[Close]

BLAST of CcUC05G087860 vs. ExPASy Swiss-Prot
Match: B9RT04 (CASP-like protein 1E1 OS=Ricinus communis OX=3988 GN=RCOM_0680180 PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.2e-35
Identity = 87/185 (47.03%), Postives = 124/185 (67.03%), Query Frame = 0


Query: 10  MEDGKNEEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLHPL 69
           M+  ++  MK+ G   N+    +V RVL FVL+  AAIV G+N QT+ VP+ ++ ++ PL
Sbjct: 12  MDGIESRGMKERGGRTNS---FLVLRVLAFVLTSTAAIVHGVNNQTETVPIQLTSSMPPL 71

Query: 70  DYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLIIVFDTVMAALL 129
                AKWHYLSAFV+ +  N IA SY+ +S+ L    K     +  +I+  D +M ALL
Sbjct: 72  YVPVVAKWHYLSAFVFFVVSNAIACSYAAISVMLSFCGKKS---MVPIILTLDLLMVALL 131

Query: 130 FSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVLASV 189
           FSS+GAA A+GV+ Y GNSHV+WNKVC+++G+FC QVAAS VLSL G++VF+ LV+L + 
Sbjct: 132 FSSNGAATAIGVMGYKGNSHVKWNKVCNVFGKFCNQVAASVVLSLIGSIVFVLLVMLTAF 190

Query: 190 GLQKR 195
            L  +
Sbjct: 192 RLHNK 190

[Close]

BLAST of CcUC05G087860 vs. ExPASy Swiss-Prot
Match: B9HMP6 (CASP-like protein 1E1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_820934 PE=3 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 1.6e-34
Identity = 80/164 (48.78%), Postives = 115/164 (70.12%), Query Frame = 0


Query: 31  EMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLHPLDYTFTAKWHYLSAFVYLLAIN 90
           +++ RVL  VL+  AAIV+G++KQTKVVP+ +   L  ++   +AKWHYLSAF Y +A N
Sbjct: 31  DLLLRVLALVLTLAAAIVLGVDKQTKVVPIKIVDTLPAINLPVSAKWHYLSAFTYSVASN 90

Query: 91  IIASSYSFVSLFLLLKNKSKDNILGLLIIVFDTVMAALLFSSSGAAAAVGVIAYHGNSHV 150
            IA SY+ +SL L +  K     +  ++IV D +M A+LFSS+GAA A+G++ Y GNSHV
Sbjct: 91  AIACSYAALSLVLAVSGKKG---IMSIVIVLDLLMVAMLFSSNGAALAIGLMGYQGNSHV 150

Query: 151 QWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVLASVGLQKR 195
           +W KVC ++GRFC QVA S  LSL G+++F+ LV + S+ L K+
Sbjct: 151 RWTKVCHVFGRFCNQVAVSISLSLLGSILFLLLVGITSLRLHKK 191

[Close]

BLAST of CcUC05G087860 vs. ExPASy TrEMBL
Match: A0A0A0LAI6 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G151490 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.1e-82
Identity = 177/198 (89.39%), Postives = 181/198 (91.41%), Query Frame = 0


Query: 1   MEVERRRVKM-EDGKNEEMKKMGN-YDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVV 60
           MEVERRR KM E+ KNEEMKKMGN YDN +KVEMVFRVLGFVLSFVAAIVVGLN QTKVV
Sbjct: 1   MEVERRRGKMEEERKNEEMKKMGNDYDNYHKVEMVFRVLGFVLSFVAAIVVGLNNQTKVV 60

Query: 61  PLTVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLI 120
           PL VSLN  PLDYTF AKWHYLSAFVYLLA NIIA SYSF+SLFLLLKNKSKDNILGLLI
Sbjct: 61  PLAVSLNSPPLDYTFIAKWHYLSAFVYLLATNIIACSYSFLSLFLLLKNKSKDNILGLLI 120

Query: 121 IVFDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAV 180
           IV DTVM ALLFS SGAA AVGVIAY GN+HVQWNKVCDIYGRFCKQVAASTVLSLAGAV
Sbjct: 121 IVLDTVMVALLFSGSGAAGAVGVIAYQGNTHVQWNKVCDIYGRFCKQVAASTVLSLAGAV 180

Query: 181 VFMSLVVLASVGLQKRPN 197
           VFMSLVVLASVGLQKRPN
Sbjct: 181 VFMSLVVLASVGLQKRPN 198

[Close]

BLAST of CcUC05G087860 vs. ExPASy TrEMBL
Match: A0A1S3AWX9 (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103483799 PE=3 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 7.4e-80
Identity = 176/201 (87.56%), Postives = 180/201 (89.55%), Query Frame = 0


Query: 1   MEVERRRVKM-EDGKNEEMK--KMGNYDNN--NKVEMVFRVLGFVLSFVAAIVVGLNKQT 60
           +E ERRR KM E+ KNEEMK  KMGNY +N  +KVEMVFRVLGFVLSFVAAIVVGLN QT
Sbjct: 3   VETERRRGKMEEERKNEEMKKIKMGNYYDNYYHKVEMVFRVLGFVLSFVAAIVVGLNNQT 62

Query: 61  KVVPLTVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILG 120
           KVVPLTVSLN  PLDYTF AKWHYLSAFVYLLA NIIA SYSFVSLFLLLK KSKDNILG
Sbjct: 63  KVVPLTVSLNTPPLDYTFIAKWHYLSAFVYLLATNIIACSYSFVSLFLLLKKKSKDNILG 122

Query: 121 LLIIVFDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLA 180
           LLIIV DTVM ALLFS SGAA AVGVIAY GNSHVQWNKVCDIYGRFCKQVAASTVLSLA
Sbjct: 123 LLIIVLDTVMVALLFSGSGAAGAVGVIAYQGNSHVQWNKVCDIYGRFCKQVAASTVLSLA 182

Query: 181 GAVVFMSLVVLASVGLQKRPN 197
           GAVVFMSLVVLASVGLQKRPN
Sbjct: 183 GAVVFMSLVVLASVGLQKRPN 203

[Close]

BLAST of CcUC05G087860 vs. ExPASy TrEMBL
Match: A0A6J1IJ94 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111474829 PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 4.8e-71
Identity = 153/195 (78.46%), Postives = 166/195 (85.13%), Query Frame = 0


Query: 1   MEVERRRVKMEDGKNEEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPL 60
           MEVERR VK  + + E+M K  N   N+  +M FRVLGFVLS VAAIVVGLNKQ+KVVPL
Sbjct: 1   MEVERRSVKKGEEEEEDMNKNKNVMVNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPL 60

Query: 61  TVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLIIV 120
           TVSLNL PLDYT  A WHYLSA VYLL+ N+IA SYSF+S   LLKNK+KDNIL LL+IV
Sbjct: 61  TVSLNLPPLDYTLIANWHYLSALVYLLSTNVIACSYSFLSFAFLLKNKNKDNILALLVIV 120

Query: 121 FDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVF 180
            DTVM ALLFSSSGAAAAVGVIAYHGNSHVQWNKVCD+YGRFCKQ+AASTVLSLAGAVVF
Sbjct: 121 LDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVF 180

Query: 181 MSLVVLASVGLQKRP 196
           M LVVLASVGLQKRP
Sbjct: 181 MLLVVLASVGLQKRP 195

[Close]

BLAST of CcUC05G087860 vs. ExPASy TrEMBL
Match: A0A6J1FS54 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448322 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 9.1e-70
Identity = 155/196 (79.08%), Postives = 167/196 (85.20%), Query Frame = 0


Query: 1   MEVERRRVKMEDGKNEE-MKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVP 60
           MEVERR VK E+ + EE M K  N   N+  +M FR LGFVLS VAAIVVGLNKQ+KVVP
Sbjct: 1   MEVERRSVKKEEEEEEEDMNK--NVMLNHMAQMGFRFLGFVLSLVAAIVVGLNKQSKVVP 60

Query: 61  LTVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLII 120
           LTVSLNL PLDYT TA WHYLSA VYLLA N+IA SYSF+S   LLKNK+KD+IL LL+I
Sbjct: 61  LTVSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDSILALLVI 120

Query: 121 VFDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVV 180
           V DTVM ALLFSSSGAAAAVGVIAYHGNSHVQWNKVCD+YGRFCKQ+AASTVLSLAGAVV
Sbjct: 121 VLDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVV 180

Query: 181 FMSLVVLASVGLQKRP 196
           FM LVVLASVGLQKRP
Sbjct: 181 FMLLVVLASVGLQKRP 194

[Close]

BLAST of CcUC05G087860 vs. ExPASy TrEMBL
Match: A0A6J1I8B2 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111472298 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.2e-69
Identity = 152/195 (77.95%), Postives = 166/195 (85.13%), Query Frame = 0


Query: 1   MEVERRRVKMEDGKNEEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPL 60
           MEVERR VK  + + E+M K  N   N+  +M FRVLGFVLS VAAIVVGLNKQ+KVVPL
Sbjct: 1   MEVERRSVKKGEEEEEDMNK--NVMVNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPL 60

Query: 61  TVSLNLHPLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKDNILGLLIIV 120
           TVSLNL PLDYT  A WHYLSA VYLL+ N+IA SYSF+S   LLKNK+KDNI+ LL+IV
Sbjct: 61  TVSLNLPPLDYTLIANWHYLSALVYLLSTNVIACSYSFLSFAFLLKNKNKDNIVALLVIV 120

Query: 121 FDTVMAALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVF 180
            DTVM ALLFSSSGAAAAVGVIAYHGNSHVQWNKVCD+YGRFCKQ+AASTVLSLAGAVVF
Sbjct: 121 LDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVF 180

Query: 181 MSLVVLASVGLQKRP 196
           M LVVLASVGLQKRP
Sbjct: 181 MLLVVLASVGLQKRP 193

[Close]

BLAST of CcUC05G087860 vs. TAIR 10
Match: AT4G15630.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 140.2 bits (352), Expect = 1.8e-33
Identity = 83/185 (44.86%), Postives = 121/185 (65.41%), Query Frame = 0


Query: 8   VKMEDGKNEEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLH 67
           ++ME GK E   + G       +E+  RVL  VL+ VAA V+G+ KQTKVVP+ +   L 
Sbjct: 12  MEMEKGKKESGSRKG-------LELTMRVLALVLTMVAATVLGVAKQTKVVPIKLIPTLP 71

Query: 68  PLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSK-DNILGLLIIVFDTVMA 127
           PL+ + TAK  YLSAFVY ++ N IA  Y+ +S+ +++ +K K    L + +++ D +M 
Sbjct: 72  PLNVSTTAKASYLSAFVYNISANAIACGYTAISIVIVMISKGKRSKSLLMAVLIGDLMMV 131

Query: 128 ALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVL 187
           ALLFSS+GAA A+G++  HGN HV W KVC ++G+FC Q A S  ++L  +VVFM LVVL
Sbjct: 132 ALLFSSTGAAGAIGLMGRHGNKHVMWKKVCGVFGKFCNQAAVSVAITLIASVVFMLLVVL 189

Query: 188 ASVGL 192
            ++ L
Sbjct: 192 DALKL 189

[Close]

BLAST of CcUC05G087860 vs. TAIR 10
Match: AT4G15620.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 137.9 bits (346), Expect = 8.8e-33
Identity = 83/185 (44.86%), Postives = 121/185 (65.41%), Query Frame = 0


Query: 8   VKMEDGKNEEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLH 67
           ++ME GK E   + G       VE+  RVL  +L+  AA V+G+ KQTKVV + +   L 
Sbjct: 12  MEMEKGKRELGSRKG-------VELTMRVLALILTMAAATVLGVAKQTKVVSIKLIPTLP 71

Query: 68  PLDYTFTAKWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKS-KDNILGLLIIVFDTVMA 127
           PLD T TAK  YLSAFVY +++N IA  Y+ +S+ +L+ ++  +   L +++++ D VM 
Sbjct: 72  PLDITTTAKASYLSAFVYNISVNAIACGYTAISIAILMISRGRRSKKLLMVVLLGDLVMV 131

Query: 128 ALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVL 187
           ALLFS +GAA+A+G++  HGN HV W KVC ++G+FC + A S  L+L  AVVFM LVVL
Sbjct: 132 ALLFSGTGAASAIGLMGLHGNKHVMWKKVCGVFGKFCHRAAPSLPLTLLAAVVFMFLVVL 189

Query: 188 ASVGL 192
            ++ L
Sbjct: 192 DAIKL 189

[Close]

BLAST of CcUC05G087860 vs. TAIR 10
Match: AT5G44550.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 76.3 bits (186), Expect = 3.1e-14
Identity = 51/171 (29.82%), Postives = 93/171 (54.39%), Query Frame = 0


Query: 29  KVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLHPLDYTFTAKWHYLSAFVYLLA 88
           K+ +  R+L F  +  AAIV+GLNK+TK   +   +   P+  TFTAK+ +  AFV+ + 
Sbjct: 17  KILLGLRLLAFSATLSAAIVMGLNKETKTF-IVGKVGNTPIQATFTAKFDHTPAFVFFVV 76

Query: 89  INIIASSYSFVSLFLLLKNKSKDNILG---LLIIVFDTVMAALLFSSSGAAAAVGVIAYH 148
            N + S ++ + + L +    K    G   L + + D +   L+ +++ AAA +  +  +
Sbjct: 77  ANAMVSFHNLLMIALQIFG-GKMEFTGFRLLSVAILDMLNVTLISAAANAAAFMAEVGKN 136

Query: 149 GNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVLASVGLQKRPN 197
           GN H +W+K+CD +  +C   A + + + AG V+ M ++  AS+    +PN
Sbjct: 137 GNKHARWDKICDRFATYCDHGAGALIAAFAG-VILMLIISAASISRLVQPN 184

[Close]

BLAST of CcUC05G087860 vs. TAIR 10
Match: AT4G20390.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 74.3 bits (181), Expect = 1.2e-13
Identity = 50/176 (28.41%), Postives = 97/176 (55.11%), Query Frame = 0


Query: 16  EEMKKMGNYDNNNKVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLHPLDYTFTA 75
           E++   G    + K+ +  R+  F+ +  AAIV+ LNK+TK + +  ++   P+  T TA
Sbjct: 4   EKIVVAGGTTKSWKLLLGLRIFAFMATLAAAIVMSLNKETKTL-VVATIGTVPIKATLTA 63

Query: 76  KWHYLSAFVYLLAINIIASSYSFVSLFLLLKNKSKD--NILGLLIIVFDTVMAALLFSSS 135
           K+ +  AFV+ +  N++ S ++ + + + + ++  +   +  L I + D + A L+ +++
Sbjct: 64  KFQHTPAFVFFVIANVMVSFHNLLMIVVQIFSRKLEYKGLRLLSIAILDMLNATLVSAAA 123

Query: 136 GAAAAVGVIAYHGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVLASV 190
            AA  V  +  +GN H +WNKVCD +  +C   A + + + AG V+ M LV   S+
Sbjct: 124 NAAVFVAELGKNGNKHAKWNKVCDRFTTYCDHGAGAIIAAFAG-VILMLLVSAVSI 177

[Close]

BLAST of CcUC05G087860 vs. TAIR 10
Match: AT3G06390.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 71.6 bits (174), Expect = 7.8e-13
Identity = 51/165 (30.91%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 29  KVEMVFRVLGFVLSFVAAIVVGLNKQTKVVPLTVSLNLHPLDYTFTAKWHYLSAFVYLLA 88
           K++++ RVL F  +  A IV+  + QT++  L    +  P+    +A+++   AF+Y + 
Sbjct: 36  KIDIITRVLLFSATLTALIVMVTSDQTEMTQLPGVSSPAPV----SAEFNDSPAFIYFVV 95

Query: 89  INIIASSY----SFVSLFLLLKNKSKDNILGLLIIVFDTVMAALLFSSSGAAAAVGVIAY 148
             ++AS Y    + VS+ LLLK +       + +   D VM  +L S++G A  V  IA 
Sbjct: 96  ALVVASFYALISTLVSISLLLKPEFTAQ-FSIYLASLDMVMLGILASATGTAGGVAYIAL 155

Query: 149 HGNSHVQWNKVCDIYGRFCKQVAASTVLSLAGAVVFMSLVVLASV 190
            GN  V WNK+C++Y +FC+ +A S  LSL  +++ + L + +++
Sbjct: 156 KGNEEVGWNKICNVYDKFCRYIATSLALSLFASLLLLVLSIWSAL 195

The following BLAST results are available for this feature:

BLAST of CcUC05G087860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879800.1	3.5e-84	90.86	CASP-like protein 1E2 [Benincasa hispida]	[more]
XP_004134188.2	4.3e-82	89.39	CASP-like protein 1E2 [Cucumis sativus] >KGN57071.1 hypothetical protein Csa_010...	[more]
XP_008438805.1	1.5e-79	87.56	PREDICTED: CASP-like protein 1E2 [Cucumis melo]	[more]
XP_022975463.1	1.0e-70	78.46	CASP-like protein 1E2 [Cucurbita maxima]	[more]
KAG7028955.1	6.5e-70	79.49	hypothetical protein SDJN02_10138 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CcUC05G087860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C6SZP8	2.8e-39	52.44	CASP-like protein 1E2 OS=Glycine max OX=3847 PE=2 SV=1	[more]
C6TBD0	8.0e-39	51.83	CASP-like protein 1E1 OS=Glycine max OX=3847 PE=2 SV=1	[more]
A7NW78	3.7e-36	50.61	CASP-like protein 1E2 OS=Vitis vinifera OX=29760 GN=VIT_05s0020g01820 PE=2 SV=1	[more]
B9RT04	3.2e-35	47.03	CASP-like protein 1E1 OS=Ricinus communis OX=3988 GN=RCOM_0680180 PE=2 SV=1	[more]
B9HMP6	1.6e-34	48.78	CASP-like protein 1E1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_820934 PE=3 S...	[more]

BLAST of CcUC05G087860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAI6	2.1e-82	89.39	CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G151490 PE=3 SV=1	[more]
A0A1S3AWX9	7.4e-80	87.56	CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103483799 PE=3 SV=1	[more]
A0A6J1IJ94	4.8e-71	78.46	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111474829 PE=3 SV=1	[more]
A0A6J1FS54	9.1e-70	79.08	CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448322 PE=3 SV=1	[more]
A0A6J1I8B2	1.2e-69	77.95	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111472298 PE=3 SV=1	[more]

BLAST of CcUC05G087860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15630.1	1.8e-33	44.86	Uncharacterised protein family (UPF0497)	[more]
AT4G15620.1	8.8e-33	44.86	Uncharacterised protein family (UPF0497)	[more]
AT5G44550.1	3.1e-14	29.82	Uncharacterised protein family (UPF0497)	[more]
AT4G20390.1	1.2e-13	28.41	Uncharacterised protein family (UPF0497)	[more]
AT3G06390.1	7.8e-13	30.91	Uncharacterised protein family (UPF0497)	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006702	Casparian strip membrane protein domain	PFAM	PF04535	DUF588	coord: 28..179 e-value: 1.9E-40 score: 138.1
IPR006459	Casparian strip membrane protein	TIGRFAM	TIGR01569	TIGR01569	coord: 33..188 e-value: 1.6E-39 score: 133.2
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 26..194
None	No IPR available	PANTHER	PTHR11615:SF253	CASP-LIKE PROTEIN 1E1	coord: 26..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC05G087860.1	CcUC05G087860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding