CcUC05G083850 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC05G083850
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionEnt-kaurenoic acid oxidase 2-like
LocationCicolChr05: 2436085 .. 2443130 (-)
RNA-Seq ExpressionCcUC05G083850
SyntenyCcUC05G083850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGAAAATAGCCATCTCCTTTTTTGATAAGTAAATAAATTTTTTTCTTTGTTTTGGTTGAAAATTAGATGGAGGTGAGTTGGGCTGTGGCGGCGGTAGCTGCAGCAGCGGCAGTGTTTGCGGCGGTGAAATGGGCGGCGAAGAGTTTGAATGAGTGGATATATGAGGCGAAATTAGGTGATCGGCGGATGGCTCTGCCGCCGGGGGATTTGGGATGGCCTATAATTGGAAATATGTTGGGTTTTCTTAGAGCTTTCAAGTCCAAGAACCCTGAAACCTTCATTGATAGCTATGTTTCCAGGTCATTAATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTCTTCTATTTTATTTTATTTTTTATTTCTAATTTCTCTATCTAGAGATAATTGGGAGCGGATATTTCAATGCGAGTGTCAGTCTCTCAACCGTTACATGCATGGTTCAATGTATTGACATCTCTTTTTGTTGGTTAGTTCAACAACAAAAGAGTGTCTAAAATAAGGAATGAAATTGTTAGTTGTGGGACTCCCAGTGTGAGAGTTATAAGTCATGATATTTTTTTGGTCGTGGAGTCCACCAACTCCTTGGAGCCGAACATGCCTTATGCGAAGTCGGTTAATCGAGCACATATGTCTTGACTATTTGTGAAAGACTCAAATTTACTTTTAGGGTGAATTTTTTTTCCTTTTTTGTGGATATTTTTTATTTTTCTTTCTCTAAAAGTTGTTGGGAGCTAATACCTCGGTGAGTGTGTTGGCCTTGTAACTGATGCATGCAAATACTAAAGTGAGCTTGATTCAATGGTATTAACATGCACTCGGTCTCTTGAGGTCGGAGGTTTAATTTCTCCACTTTGCAATTTTTGTACTAAAAAGATTGATGTTTTATTCTTTTTATTACACATAAATGATCTTTTTCCCATTCGGGGAAAATTTAAAATTTTGACATTTTAAAACTTTTAACCTTTCAAGGTTTTCAATACAGGAAGGTTTACCTTTTTGTTTTCCTTTCTTTTCTCACTATATGCATGTGTGTCAAGTTTGATTGATTGAAAATACATTAAATTTATTTTACTAGAACAAACTTCTAGATATGAGAACCAAAACGACATCCAACCTTAAGAAAACGTGGTTAGGATTTAGAGTTATTAACTCCACTTCTTATTCGGTTTAAAGAGTTTATGGACCTTATAACTAAAAGGCCTCGAGTCTACGTCTTATTAATTAGTATAAAGTTAATAAGACTCAACAATTTGCAAATTTCTAGACTTTACAATCTCTTTCCTTTGTCTCGAACTCTCATATTTTTTCTCACATTCTCACCAAAGAAGGTATTGGGAGAGAATGCAATGACGTTAGCTTTATTTGGTTTTTTCCTTTTTCAATGCATGCATGTTAATTATTGTTAAAAATGATTATATATATATATTAACACAATAAATTCTTATAGGTTGAATGGTGTATTCCTATTTTTTCTCTGTCTCATATCTGACAGGAATAAAATTTCTGGTTTGAAAATTGTCTAAAGAGGATGAGGATATTTTAGTACTTTTGGAGGTTGATGAGTTCAAATAGCTAATTTGTATTTGATTTTTAACAATATCTTTCTTCCTTCTGAAGAATTCCTTTTCTCAAAGTTCTATTTTTTCTTCTTATTAGTTCTTATCTGTCCTTACTTGATTTCAAATGATAAGAAGAAATCAATTTTTATTCCAAAAAGTTTCTGCATTTTCATGACCTTACCAATTGCTTTAGGTTACTATTAACCCTTCAATTTTCATTAAAAGGGTTTCAAAATTTTTACATGTAATATTTCAATTCTAAAATTAGAGAAAATTAGAAAAATAATTAAACTTTTAGTTTTGAATTTAGAATATACTTTTTTTTAAGAAGAAAAAGCAAAGGACAGGGGACCAAATTACTATAATGACCCTGAAAGCGTTTTTAACCGGCATGCATGAAAAATGTGGAGAACCGTGAGATAGTGACGTCAGCAAACAGAGAAACGGTGAACTTGATTCAAGACCAAATTATCTCTCTTTTTTTTTCCCCTTTCTTTGTCCATTTCTAATTATATTAATTTTTAAATAAACAATTCCAATATTATTATAAGTTTATTTAGATTCACATTTTAGGTGCTTAAAAGTATTTTTATTACCTTTGAAGGATATCGAGATAAAAAGTGAAGTTTTGAAGTCTAGATAGAGTTGTTAAGTCGTGAGGTTCACTTAGTCATATTCTTTAATTGTGGGACACACGAACTCTTGGACCAAACAAAGAGGAAAATTTTTAGTACTTCCATAGTAGCATACTCGCTTTGTTCACATTATAAATATGAAAGATATTTATAGTGGGACACACGAAAGTTCTGAAGTCTAGACAGAATTGTTAAGTCATGGGGTTCACTTAGTTATATTTTTTAATTGTGGGACACACGAACTCTTCGACCAAACAAAGAGGAGAATTTTTAGTGCTCGCATAGCAGCATACTCGCTTTGTTCACATTATAAATATAAAAGATGTGGACCCCATATTGATACTAGTGGGTCCCACCCTATTTTTAAAAACACCTTAATACACAATGGCCCCAAACAAAAAGTGGATTTGTGAATTCCACATTCCCCATCTCTAACTTCTTTGGCCAAACATCTCTGAAAAATCAATCCACGTTGACCATAAATATTGAGTCGAATTTAATTAAGAATTGAGTAATCAACTCAACTCAATTCACCAACACTTATAGTTAATATAATTCAATATATAAATAAAAAAATTGTTATTCTAAAAGTCGACAATTTCATTTTCTTAACCTTTAAAAAGAAACTCTCAAATCTGAGAAAAACATGAGATTTTATCTCCGGCAAGAATGTGTCTCTTTGAGTATATCATTTTTGGGTTGGTTCACTTTTTTTTTTTTTAACTAAATATTTGTTTAAACTTCATAAACTCTATCACTATATTTGACAAATATGGAATTTTATCTAGAGACCAATAAAGAATGATCATATTAGACTCTCCATCTTTTCAAGTAAAATCTTCAACAACATCTAACATTTTAACTACTTAACTTGGAAGTATGTTTTTGTTTATATATATTTTAAATATTCTTGATTGTTGGAGTTAGTGGTACGGTTGATAATATTTTATAATCATATAACACTCAAGTTTTTACCCTTTTTTTTATTTTTATTTTTTATTTTATTAACAAACTCTCGTGCTATGATTTCAGTCTTGTAGTTTTTGTTGAGTATTGAATAACAAAAATGGTTCTCAACTCAATAATTCTATTTAGAATTTCCAAGATAATTTTTGAACAATATATTCCTATTTTACATATTTCTTAAATAATAAATTGAAAATTGTATTTGTTCGGATAATTTCCATGTCAGTATGGTTCCTTGATCGATGATGCGACTTATTTATTTATTTGTTTATTTAATTTAGTTTATTTTAGTTGGTTTTACCTTCTGGTGAAAAGTGGACAATTTCTTGTGTTGATCTTATGTTTGCTCAATTCTAGGAATCCTTCTCATCATTCTCTAGGTTGTTCTTATAAACAATAAAATGTGTGTATATATATATTTGAAATTAGATTTTGAACATATAATGAATTATATCATGATTTATTTGAAAGGAAACATGTCATTTCACTTGATGATAAGATAGTTTATTAGTGTAATTTGGTGTTTGCTTAATTTCCATGATATATTTTTAAATGTCTAATTTTAATAATTTAGTCTATTTCATTACTTTATTGTTAATTTTTTTTTCATAAACTTGTTTCTTATCTATTAGTAGTTGCATTATAAACATTGTAAACATATTCGTATATTGTGCCTTATTACATAAAAATTATTATTATTATTTAATTAATTTCAATCAAAATCAATTTTGATGAATTAAATTTAAAATTTACTAAAAGTATATTCTCAATTGTAATTGATTATTCTTTCAAAATAATTTTGATTTTGATTTAAAGACAATAATATTTTATTTTATTTTATTTTTTAAATTGTTGAAGCCATAAAAAGAGTCTTGAAAAGTGGGAAACTAAAAGAAAAATTATTAAACAACCTAATGTTTTAAAGCATAGTATTAAAAATATAACTACCATTAAAATGCACCTTATTTTTGTGTACATTACTCTTAACTTTTTATATTTAACAAAATGATATAGTTAAGAGGTTGTTTTCAAATATAGAAAAATGAACCAAAATTATACAAATATAACAAAATTTTACTATCTATCGACGATAGACCACGACAGATTATCTGTGTCATGATATATATAGATAATAGTATATCACAGTTTACTTATAGTAGATTGTGATATTTTACTATTATTTGTACATATTTTCAACAATTTTGTAAAAATAATTTTCCCCTGTTTAAAGTATCTGGGTTGAAAATTTTGTTATTAAGTTTGAAATTAAATATTAAGACTAATTTTTCCAAAAAGGAAAAAAAGAACTTTGTTCTTGAATCAGTTTGAGATTTAAAAAAAAAAAAAAAAAGAAAGTTATCAATAATGAGACGATAAAAAATCTAATTCTATTAGTTTTCTTTTTAAAAAATATAATTATATTAATTAATTATTTACTCAACAATTAATTACAGGTTTGGGAAAATAGGAGTGTACAAGGTTCATTTGTTTGGAAACCCTAGCGTGGTCGTGACAACGCCGGAGACATGCCGGAAAGTTCTTACAGACGACGAGGCCTTTCAGCCCGGTTGGCCACGCGCGGCCATAGAGCTCATCGGAAAGAAGTCCTTCATCGAAATGCCGGTCGAGGAACACAAGCGCCTTCGCCGCTTAACCTCTGCTCCGGTGAACGGTTTCGAGGCTTTGAGCAATTACATTCCTTACATCGAAGAGAATGTATTGAAATCGTTGGATAAATGGTCCAATATGGGGCCAATCGAATTCTTAACTCAACTTCGTAAGCTCACATTCACCATTATCATGTACATTTTCCTTAGCGCGGAGAGCGAATCGGTAATGGAGGCGTTAGAGAAGGAGTACACTCGGCTGAACTACGGTGTTCGAGCCCTGAGAATTAACATACCTGGATTTGCTTACCACAGAGCACTCAAGGTATTTGATAATTTGCCTCGATCAATTTTGACAATGTACTTTGGATTCTGGAATTTTTTCGATCAATTAAGCCACCGGCTACATGTTTAGGCTCGGAAGAATCTTGTGGCTGCGTTGCAAGCAATTGTTACTGATCGAAGAAAACGAAGATTGGACAAATGGGCCCCGATGAGGAAGGACATGATGGACGCTCTGATTGATGTTGAAGATGAAAATGGCAGAAAATTGACTGATGAGGAGATCATTGATATCTTAGTGATGTACTTGAATGCTGGTCATGAATCTTCAGGCCATACCATGATGTGGGCAACTATTTTGTTGAATCAACACCCAAAAGTTCTCAAGAAAGCTAAGGTATATACACAATTAGGACCTTAATTAGCTTGTTTTGTTACTCAAAATCATGCCATATCTTCATATTGAGTGATCAATCATGATAAAAATTGGTTTAATAAATTGAACAGGCAGAGCAGGAGGAGATTGTGAGGAGGAGACCTCCAGGTCAAAAGGGATTGACTTTGAAAGAATGCAGGGAAATGGAATATCTCTCAAAAGTGGTCGACGAGACTCTTCGCTATGTATCATTCTCCCTTGTTGTCTTCAGAGAAGCACAGATGGATGTCAATCTTAATGGTACCTCTCTCTCTCATTTGAATTGGAACAATGTGATTGACGGTTTTATACCATTAAATAATTGCGAGATATCAAAGTTTTGGTTTAACTATGCATCGAGAAATAATTCGAAGAGGCCCACTCTAATGTGGCTAGTAATTTGCATGAGAGAGGAACTATAAATATATTTCGAATTTATGCCTAAAGGTTATTAGAAACATTCAGGAGGTTGTTTGGGGCGCCAAGTTGGTTATTATAGTCTTGTGGGTTATAGTAATCTGTGTTTGGGGGTGCAGCCTATTCTAGTCTAGTTACAAATGAGAAGAGATGATGGTTACAAAATAGTAAATGACAATGAGAGCTTTCAAATAGTTTTACTGTAGTTAAGTATGAGTTATAATAATTGGAAACTCCAACTATTATAATGGGAGACTCCAACATTGAGTAGGCTATCAAGACAAGTTGGGAGTCCAAATGCCTCCTTAGGTTTTTGAATCCATGAATAACTTTTCCCATGCATCCAAGTGTTAGGAGGGAGAGCGGCACTAAATAATTATTTCCTCTTGTTTGTCTCTCCATTGTATCAATGGTCTTTGTCCGGTTGCAGGCTATCTAATTCCCAAGGGTTGGAAAGTCCTGGCTTGGTTCAGAAGCATTCACTATGACAGTGAAGTTTACCCAGACCCAAAGAAATTTGATCCTTCAAGATGGGATGTAAGTTAAAAACTCAGTTTCAAAATCCTTTTACAAGAATTCAAGTTACATCACATCAAATGAAATCATTTCTAATATGTGTGATATCTACTCTGTGTTTGGCATAAAGGGATTTATCCCAAAAGCAGGTGAGTTTCTTCCATTTGGAGCAGGAAGCAGGCTGTGCCCAGGAAATGATCTAGCCAAATTGGAGATCTGCATTTTCATTCACTATTTTCTCCTCAATTACAAGTAAGTTCACTTGGAAAATACATATTCCTTGTTCCTTTTTTCTAGTTCACATCCTTTGAAAACACAGAAGCTAATGAGTTTTTTTTCTTAACATTTTCTGTTGTTGGTTGGGTTGAAATTTACAGGCTTGAATGGCTGACCCCTGATTGTCCAATCTTGTATCTGCCACATTCAAGGCCAAAGGACAATTGCTTGGCTAAAATCAGTAAGAATTCCAGCACTGTTGTGTAAGGAGAGTTTTTACCTTTCATGTAGTATAAATGGGTGTTGTACTGTTAATGCCCCACACAGAAAGAGACATGCATGTCAAATCTTGGCTTCTTCTTGTGTCATTTGTAGTGAATTTTGTACTGGAATTCTGGTTCAAATAAATTAGAAGTTACTGCAGT

mRNA sequence

AAGGAAAATAGCCATCTCCTTTTTTGATAAGTAAATAAATTTTTTTCTTTGTTTTGGTTGAAAATTAGATGGAGGTGAGTTGGGCTGTGGCGGCGGTAGCTGCAGCAGCGGCAGTGTTTGCGGCGGTGAAATGGGCGGCGAAGAGTTTGAATGAGTGGATATATGAGGCGAAATTAGGTGATCGGCGGATGGCTCTGCCGCCGGGGGATTTGGGATGGCCTATAATTGGAAATATGTTGGGTTTTCTTAGAGCTTTCAAGTCCAAGAACCCTGAAACCTTCATTGATAGCTATGTTTCCAGGTTTGGGAAAATAGGAGTGTACAAGGTTCATTTGTTTGGAAACCCTAGCGTGGTCGTGACAACGCCGGAGACATGCCGGAAAGTTCTTACAGACGACGAGGCCTTTCAGCCCGGTTGGCCACGCGCGGCCATAGAGCTCATCGGAAAGAAGTCCTTCATCGAAATGCCGGTCGAGGAACACAAGCGCCTTCGCCGCTTAACCTCTGCTCCGGTGAACGGTTTCGAGGCTTTGAGCAATTACATTCCTTACATCGAAGAGAATGTATTGAAATCGTTGGATAAATGGTCCAATATGGGGCCAATCGAATTCTTAACTCAACTTCGTAAGCTCACATTCACCATTATCATGTACATTTTCCTTAGCGCGGAGAGCGAATCGGTAATGGAGGCGTTAGAGAAGGAGTACACTCGGCTGAACTACGGTGTTCGAGCCCTGAGAATTAACATACCTGGATTTGCTTACCACAGAGCACTCAAGGCTCGGAAGAATCTTGTGGCTGCGTTGCAAGCAATTGTTACTGATCGAAGAAAACGAAGATTGGACAAATGGGCCCCGATGAGGAAGGACATGATGGACGCTCTGATTGATGTTGAAGATGAAAATGGCAGAAAATTGACTGATGAGGAGATCATTGATATCTTAGTGATGTACTTGAATGCTGGTCATGAATCTTCAGGCCATACCATGATGTGGGCAACTATTTTGTTGAATCAACACCCAAAAGTTCTCAAGAAAGCTAAGGCAGAGCAGGAGGAGATTGTGAGGAGGAGACCTCCAGGTCAAAAGGGATTGACTTTGAAAGAATGCAGGGAAATGGAATATCTCTCAAAAGTGGTCGACGAGACTCTTCGCTATGTATCATTCTCCCTTGTTGTCTTCAGAGAAGCACAGATGGATGTCAATCTTAATGGCTATCTAATTCCCAAGGGTTGGAAAGTCCTGGCTTGGTTCAGAAGCATTCACTATGACAGTGAAGTTTACCCAGACCCAAAGAAATTTGATCCTTCAAGATGGGATGGATTTATCCCAAAAGCAGGTGAGTTTCTTCCATTTGGAGCAGGAAGCAGGCTGTGCCCAGGAAATGATCTAGCCAAATTGGAGATCTGCATTTTCATTCACTATTTTCTCCTCAATTACAAGCTTGAATGGCTGACCCCTGATTGTCCAATCTTGTATCTGCCACATTCAAGGCCAAAGGACAATTGCTTGGCTAAAATCAGTAAGAATTCCAGCACTGTTGTGTAAGGAGAGTTTTTACCTTTCATGTAGTATAAATGGGTGTTGTACTGTTAATGCCCCACACAGAAAGAGACATGCATGTCAAATCTTGGCTTCTTCTTGTGTCATTTGTAGTGAATTTTGTACTGGAATTCTGGTTCAAATAAATTAGAAGTTACTGCAGT

Coding sequence (CDS)

ATGGAGGTGAGTTGGGCTGTGGCGGCGGTAGCTGCAGCAGCGGCAGTGTTTGCGGCGGTGAAATGGGCGGCGAAGAGTTTGAATGAGTGGATATATGAGGCGAAATTAGGTGATCGGCGGATGGCTCTGCCGCCGGGGGATTTGGGATGGCCTATAATTGGAAATATGTTGGGTTTTCTTAGAGCTTTCAAGTCCAAGAACCCTGAAACCTTCATTGATAGCTATGTTTCCAGGTTTGGGAAAATAGGAGTGTACAAGGTTCATTTGTTTGGAAACCCTAGCGTGGTCGTGACAACGCCGGAGACATGCCGGAAAGTTCTTACAGACGACGAGGCCTTTCAGCCCGGTTGGCCACGCGCGGCCATAGAGCTCATCGGAAAGAAGTCCTTCATCGAAATGCCGGTCGAGGAACACAAGCGCCTTCGCCGCTTAACCTCTGCTCCGGTGAACGGTTTCGAGGCTTTGAGCAATTACATTCCTTACATCGAAGAGAATGTATTGAAATCGTTGGATAAATGGTCCAATATGGGGCCAATCGAATTCTTAACTCAACTTCGTAAGCTCACATTCACCATTATCATGTACATTTTCCTTAGCGCGGAGAGCGAATCGGTAATGGAGGCGTTAGAGAAGGAGTACACTCGGCTGAACTACGGTGTTCGAGCCCTGAGAATTAACATACCTGGATTTGCTTACCACAGAGCACTCAAGGCTCGGAAGAATCTTGTGGCTGCGTTGCAAGCAATTGTTACTGATCGAAGAAAACGAAGATTGGACAAATGGGCCCCGATGAGGAAGGACATGATGGACGCTCTGATTGATGTTGAAGATGAAAATGGCAGAAAATTGACTGATGAGGAGATCATTGATATCTTAGTGATGTACTTGAATGCTGGTCATGAATCTTCAGGCCATACCATGATGTGGGCAACTATTTTGTTGAATCAACACCCAAAAGTTCTCAAGAAAGCTAAGGCAGAGCAGGAGGAGATTGTGAGGAGGAGACCTCCAGGTCAAAAGGGATTGACTTTGAAAGAATGCAGGGAAATGGAATATCTCTCAAAAGTGGTCGACGAGACTCTTCGCTATGTATCATTCTCCCTTGTTGTCTTCAGAGAAGCACAGATGGATGTCAATCTTAATGGCTATCTAATTCCCAAGGGTTGGAAAGTCCTGGCTTGGTTCAGAAGCATTCACTATGACAGTGAAGTTTACCCAGACCCAAAGAAATTTGATCCTTCAAGATGGGATGGATTTATCCCAAAAGCAGGTGAGTTTCTTCCATTTGGAGCAGGAAGCAGGCTGTGCCCAGGAAATGATCTAGCCAAATTGGAGATCTGCATTTTCATTCACTATTTTCTCCTCAATTACAAGCTTGAATGGCTGACCCCTGATTGTCCAATCTTGTATCTGCCACATTCAAGGCCAAAGGACAATTGCTTGGCTAAAATCAGTAAGAATTCCAGCACTGTTGTGTAA

Protein sequence

MEVSWAVAAVAAAAAVFAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNMLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVV
Homology
BLAST of CcUC05G083850 vs. NCBI nr
Match: XP_038902869.1 (ent-kaurenoic acid oxidase 1-like [Benincasa hispida])

HSP 1 Score: 972.6 bits (2513), Expect = 1.3e-279
Identity = 476/492 (96.75%), Postives = 483/492 (98.17%), Query Frame = 0

Query: 1   MEVSWAVAAVAAAAAVFAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNMLGFL 60
           MEVSWAVAAVAAA A FAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWP+IGNML FL
Sbjct: 1   MEVSWAVAAVAAAVAAFAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGNMLAFL 60

Query: 61  RAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRA 120
           RAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPR+
Sbjct: 61  RAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRS 120

Query: 121 AIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIE 180
           AIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEE+VLKSL KWS+MGPIE
Sbjct: 121 AIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEESVLKSLGKWSDMGPIE 180

Query: 181 FLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARK 240
           FLTQLRKLTFTIIMYIFLSAESE VMEALEKEYTRLNYGVRALRIN PGFAYH+ALKARK
Sbjct: 181 FLTQLRKLTFTIIMYIFLSAESEPVMEALEKEYTRLNYGVRALRINFPGFAYHKALKARK 240

Query: 241 NLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGH 300
           NLVAALQ IVTDRRKRRLDKWAP RKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGH
Sbjct: 241 NLVAALQRIVTDRRKRRLDKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGH 300

Query: 301 ESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDET 360
           ESSGHTMMWATILLNQHP+VLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDET
Sbjct: 301 ESSGHTMMWATILLNQHPEVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDET 360

Query: 361 LRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFI 420
           LRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWD F+
Sbjct: 361 LRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDNFV 420

Query: 421 PKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNC 480
           PKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNC
Sbjct: 421 PKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNC 480

Query: 481 LAKISKNSSTVV 493
           LAKISKNSSTVV
Sbjct: 481 LAKISKNSSTVV 492

BLAST of CcUC05G083850 vs. NCBI nr
Match: TYK17483.1 (ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 960.3 bits (2481), Expect = 6.4e-276
Identity = 473/497 (95.17%), Postives = 481/497 (96.78%), Query Frame = 0

Query: 1   MEVSW----AVAAVAAAAAV-FAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGN 60
           MEVSW    AV  VA AAAV FA VKWAAKS NEWIYEAKLG+RRMALPPGDLGWP+IGN
Sbjct: 2   MEVSWGTAAAVTVVAGAAAVFFAVVKWAAKSFNEWIYEAKLGERRMALPPGDLGWPLIGN 61

Query: 61  MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 120
           MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP
Sbjct: 62  MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 121

Query: 121 GWPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN 180
           GWPRAA+ELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS 
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWST 181

Query: 181 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRA 240
           MGPIEFLTQLRKLTFTIIMYIFLSAESESVME+LEKEYTRLNYGVRALRIN+PGFAYH+A
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKA 241

Query: 241 LKARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMY 300
           LKARKNLVAALQ IVT+RRKRRL KWAP RKDMMDALIDVEDENGRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301

Query: 301 LNAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSK 360
           LNAGHESSGHTMMWATILLNQHP+VLKKAK EQEEIVRRRPPGQKGLTLKECREMEYLSK
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSK 361

Query: 361 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSR 420
           VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421

Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 480
           WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 481

Query: 481 PKDNCLAKISKNSSTVV 493
           PKDNCLAKISKNSSTVV
Sbjct: 482 PKDNCLAKISKNSSTVV 498

BLAST of CcUC05G083850 vs. NCBI nr
Match: XP_008438234.1 (PREDICTED: ent-kaurenoic acid oxidase 2-like [Cucumis melo])

HSP 1 Score: 958.7 bits (2477), Expect = 1.9e-275
Identity = 472/496 (95.16%), Postives = 480/496 (96.77%), Query Frame = 0

Query: 1   MEVSW----AVAAVAAAAAV-FAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGN 60
           MEVSW    AV  VA AAAV FA VKWAAKS NEWIYEAKLG+RRMALPPGDLGWP+IGN
Sbjct: 2   MEVSWGTAAAVTVVAGAAAVFFAVVKWAAKSFNEWIYEAKLGERRMALPPGDLGWPLIGN 61

Query: 61  MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 120
           MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP
Sbjct: 62  MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 121

Query: 121 GWPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN 180
           GWPRAA+ELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS 
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWST 181

Query: 181 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRA 240
           MGPIEFLTQLRKLTFTIIMYIFLSAESESVME+LEKEYTRLNYGVRALRIN+PGFAYH+A
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKA 241

Query: 241 LKARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMY 300
           LKARKNLVAALQ IVT+RRKRRL KWAP RKDMMDALIDVEDENGRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301

Query: 301 LNAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSK 360
           LNAGHESSGHTMMWATILLNQHP+VLKKAK EQEEIVRRRPPGQKGLTLKECREMEYLSK
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSK 361

Query: 361 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSR 420
           VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421

Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 480
           WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 481

Query: 481 PKDNCLAKISKNSSTV 492
           PKDNCLAKISKNSSTV
Sbjct: 482 PKDNCLAKISKNSSTV 497

BLAST of CcUC05G083850 vs. NCBI nr
Match: XP_004133946.1 (ent-kaurenoic acid oxidase 1 isoform X1 [Cucumis sativus] >KAE8650281.1 hypothetical protein Csa_010193 [Cucumis sativus])

HSP 1 Score: 954.9 bits (2467), Expect = 2.7e-274
Identity = 468/497 (94.16%), Postives = 477/497 (95.98%), Query Frame = 0

Query: 1   MEVSWAVAA-----VAAAAAVFAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGN 60
           MEVSW VAA       AAA VFA +KWAAKSLNEWIYEAKLGDRRMALPPGDLGWP+IGN
Sbjct: 2   MEVSWGVAAAVTALAGAAAVVFAVLKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPLIGN 61

Query: 61  MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 120
           MLGFLRAFKS NPETFIDSYVSRFGKIGVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQP
Sbjct: 62  MLGFLRAFKSNNPETFIDSYVSRFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQP 121

Query: 121 GWPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN 180
           GWPRAA+ELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN 181

Query: 181 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRA 240
           MGPIEFLTQLRKLTFTIIMYIFLSAESESVME+LEKEYTRLNYGVRALRINIPGFAYH+A
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKA 241

Query: 241 LKARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMY 300
           LKARKNLVAA Q IVT+RRKRRL  WAP RKDMMDALIDVEDENGRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAAFQGIVTERRKRRLGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301

Query: 301 LNAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSK 360
           LNAGHESSGHTMMWATILLNQHP+VLKKAK EQEEIVRRRP GQKGLTLKECR+MEYLSK
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSK 361

Query: 361 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSR 420
           VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421

Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 480
           WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDC ILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSR 481

Query: 481 PKDNCLAKISKNSSTVV 493
           PKDNCLAKISKNSSTVV
Sbjct: 482 PKDNCLAKISKNSSTVV 498

BLAST of CcUC05G083850 vs. NCBI nr
Match: XP_022924644.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita moschata])

HSP 1 Score: 909.1 bits (2348), Expect = 1.7e-260
Identity = 436/493 (88.44%), Postives = 466/493 (94.52%), Query Frame = 0

Query: 1   MEVSWAVA----AVAAAAAVFAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNM 60
           ME +WAVA    AV A A VF+ +KWAAKSLNEWIYEAKLGDRR+ALPPGDLGWP+IGNM
Sbjct: 1   MESTWAVAAVVTAVVAVATVFSVLKWAAKSLNEWIYEAKLGDRRLALPPGDLGWPLIGNM 60

Query: 61  LGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120
           LGFLRAFKSKNPETFID YVSR+GK GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG
Sbjct: 61  LGFLRAFKSKNPETFIDGYVSRYGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120

Query: 121 WPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNM 180
           WPRAAIELIG+KSFI+MP EEHKRLRRLTSAPVNGFEALSNYIPYIE+NVL+SL+KWS M
Sbjct: 121 WPRAAIELIGEKSFIQMPEEEHKRLRRLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKM 180

Query: 181 GPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRAL 240
           GPIEFLTQLRKLTFT+IMYIFLS+ESE VME LEKEYTRLNYGVRA+RIN+PGFAYH+AL
Sbjct: 181 GPIEFLTQLRKLTFTVIMYIFLSSESEPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKAL 240

Query: 241 KARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYL 300
           KARKNLVAAL+ IVT+RR+++LDKWAP RKDMMD LID+EDENGRKL DEEIIDIL+MYL
Sbjct: 241 KARKNLVAALKGIVTERRRQKLDKWAPKRKDMMDQLIDIEDENGRKLDDEEIIDILIMYL 300

Query: 301 NAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKV 360
           NAGHESSGHTMMWATILLNQHP+VLKKA+ EQE IVR RP GQ GLTLKECR+MEYLSKV
Sbjct: 301 NAGHESSGHTMMWATILLNQHPEVLKKAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKV 360

Query: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRW 420
           VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRW
Sbjct: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRW 420

Query: 421 DGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRP 480
           DGF+PKAGEFLPFGAGSRLCPGNDLAKLEICIF+HYFLLNY LEWLTPDC ILYLPHSRP
Sbjct: 421 DGFVPKAGEFLPFGAGSRLCPGNDLAKLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRP 480

Query: 481 KDNCLAKISKNSS 490
           KDNC+AKI+K SS
Sbjct: 481 KDNCMAKITKKSS 493

BLAST of CcUC05G083850 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 1.7e-199
Identity = 316/467 (67.67%), Postives = 394/467 (84.37%), Query Frame = 0

Query: 20  VKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNMLGFLRAFKSKNPETFIDSYVSRF 79
           + W  + +N W+YE+ LG+ R  LPPGDLGWP IGNML FLRAFK+ +P++F  + + R+
Sbjct: 22  LNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRY 81

Query: 80  GKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIELIGKKSFIEMPVEEHK 139
           G  G+YK H+FGNPS++VTT +TCR+VLTDD+AF+PGWP + +ELIG+KSF+ +  EEHK
Sbjct: 82  GPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHK 141

Query: 140 RLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLS 199
           RLRRLT+APVNG EALS YIPYIEENV+  LDKW+ MG  EFLT LRKLTF IIMYIFLS
Sbjct: 142 RLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIFLS 201

Query: 200 AESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARKNLVAALQAIVTDRRKRRLD 259
           +ESE+VM+ALE+EYT LNYGVRA+ +NIPGFAYHRALKARK LVAA Q+IVT+RR +R  
Sbjct: 202 SESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQ 261

Query: 260 KWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPK 319
                +KDM+D L++V+DE+G+ L DEEIID+L+MYLNAGHESSGHT+MWAT+ L +HP+
Sbjct: 262 NILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPE 321

Query: 320 VLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVN 379
           VL++AKAEQE I++ RP GQKGL+LKE R+ME+LS+VVDETLR ++FSL  FREA+ DV 
Sbjct: 322 VLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVE 381

Query: 380 LNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWD-GFIPKAGEFLPFGAGSRLCPG 439
           +NGYLIPKGWKVL WFR +H D EV+PDP+KFDP+RWD GF+PKAG FLPFGAGS LCPG
Sbjct: 382 MNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPG 441

Query: 440 NDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKIS 486
           NDLAKLEI IF+H+FLL Y+++   P+CP++YLPH+RP DNCLA+IS
Sbjct: 442 NDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488

BLAST of CcUC05G083850 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 691.4 bits (1783), Expect = 7.3e-198
Identity = 318/467 (68.09%), Postives = 392/467 (83.94%), Query Frame = 0

Query: 20  VKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNMLGFLRAFKSKNPETFIDSYVSRF 79
           +KW  K +N WIY +KLG+++  LPPGDLGWP+IGNM  FLRAFK+ +PE+FI SY++R+
Sbjct: 21  LKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFIQSYITRY 80

Query: 80  GKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIELIGKKSFIEMPVEEHK 139
           G+ G+YK H+FG P V+VTTPETCR+VLTDD+AF  GWP++ ++LIG+KSF+ +  EEHK
Sbjct: 81  GRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHK 140

Query: 140 RLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLS 199
           RLRRLTSAPVNG EALS YI +IEE V   L+KWS MG IEFL+ LRKLTF +IMYIFLS
Sbjct: 141 RLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLS 200

Query: 200 AESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARKNLVAALQAIVTDRRKRRLD 259
           +ESE VM++LE+EYT LNYGVRA+ IN+PGFAYHRALKARK LVAA Q+IVT+RR +R  
Sbjct: 201 SESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQ 260

Query: 260 KWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPK 319
             +  RKDM+D LIDV+DENGR L DEEIID+L+MYLNAGHESSGH  MWATIL+ +HP 
Sbjct: 261 NISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPM 320

Query: 320 VLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVN 379
           +L+KAK EQE IV++R PGQK LTLKE REM YLS+V+DETLR ++FSL  FREA+ DV 
Sbjct: 321 ILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQ 380

Query: 380 LNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGN 439
           ++GY+IPKGWKVL WFR++H D E+YPDPKKFDPSRW+G+ PKAG FLPFG GS LCPGN
Sbjct: 381 MDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGN 440

Query: 440 DLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISK 487
           DLAKLEI IF+H+FLL Y++E   P CP+++LPH+RPKDNCLA+I++
Sbjct: 441 DLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of CcUC05G083850 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.2e-170
Identity = 287/492 (58.33%), Postives = 375/492 (76.22%), Query Frame = 0

Query: 5   WAVAAVAAAAAVFAAVKWAAKSLNEWIYEAKLG-DRRMALPPGDLGWPIIGNMLGFLRAF 64
           WAVAAV AA AV  A+  A ++ + W + A LG  RR  LPPGD+GWP++G M  FLRAF
Sbjct: 7   WAVAAVVAALAV-VALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAF 66

Query: 65  KSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIE 124
           KS  P++FIDS+  RFG+ G+Y+  +F +P+++ TTPE C++VL DD+AF  GWP+A + 
Sbjct: 67  KSGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVA 126

Query: 125 LIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNM---GPIE 184
           LIG KSF+ M  +EH+RLR+LT+AP+NGF+AL++Y+ +I++ V+ +L  WS     G  E
Sbjct: 127 LIGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFE 186

Query: 185 FLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARK 244
           FLT+LR++TF II+ IF+    E     LE+ YT LNYG+RA+ I++PGFAYH+A++AR+
Sbjct: 187 FLTELRRMTFRIIVQIFMGGADERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRARR 246

Query: 245 NLVAALQAIVTDRRKR--RLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNA 304
            LVAALQ ++ +RR R  +    A    DMMD LI VEDE GR+L D+EIID+LVMYLNA
Sbjct: 247 RLVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNA 306

Query: 305 GHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVD 364
           GHESSGH  MWAT+ L ++P++L KAKAEQE I+R  PPGQKGLTL++ R+M YLS+VVD
Sbjct: 307 GHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVVD 366

Query: 365 ETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDG 424
           ETLR+V+ S V FR+A  DV +NGYLIPKGWKV  W+RS+H D +VYPDPKKFDPSRW+G
Sbjct: 367 ETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEG 426

Query: 425 FIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKD 484
             P+AG FLPFG G+RLCPGNDLAKLEI +F+H+FLL YKL    P+C + YLPH RP D
Sbjct: 427 PPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPHPRPVD 486

Query: 485 NCLAKISKNSST 491
           NCLAKI++ SS+
Sbjct: 487 NCLAKITRLSSS 497

BLAST of CcUC05G083850 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.4e-163
Identity = 268/488 (54.92%), Postives = 363/488 (74.39%), Query Frame = 0

Query: 5   WAVAAVAAAAAVFAAVKWAAKSLNEWIYEAKLG-DRRMALPPGDLGWPIIGNMLGFLRAF 64
           W +AA   A  V   V    +  ++W+  A LG +RR  LPPG++GWP++G+M  FLRAF
Sbjct: 18  WVLAAAVVAGVVL--VDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAF 77

Query: 65  KSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIE 124
           KS NP+ FI S++ RFG+ GVY+  +F +P+++  TPE C++VL DDE F  GWP+A + 
Sbjct: 78  KSGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVT 137

Query: 125 LIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN--MGPIEF 184
           LIG KSF+ M  ++H+R+R+LT+AP+NGF+AL+ Y+ +I++ V+ SL +WS+   G +EF
Sbjct: 138 LIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEF 197

Query: 185 LTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARKN 244
           LT+LR++TF II+ IF+S   ++ MEALE+ YT LNYG+RA+ IN+PGFAY+RAL+AR+ 
Sbjct: 198 LTELRRMTFKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRK 257

Query: 245 LVAALQAIVTDRRKRRLDKWAPM-RKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGH 304
           LV+ LQ ++  RR      +      DMMD LI+ EDE GR+L D+EI+D+L+MYLNAGH
Sbjct: 258 LVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGH 317

Query: 305 ESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDET 364
           ESSGH  MWAT+ L ++P +  +AKAEQEEI+R  P  Q GLTL++ ++M +LS+VVDET
Sbjct: 318 ESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDET 377

Query: 365 LRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFI 424
           LR V+ S V FR+A  D+ +NGYLIPKGWKV  W+RS+H D +VYPDPK F+PSRW+G  
Sbjct: 378 LRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPP 437

Query: 425 PKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNC 484
           PKAG FLPFG G+RLCPGNDLAKLEI +F+H+FLL YKL+   P C + YLPH RP DNC
Sbjct: 438 PKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNC 497

Query: 485 LAKISKNS 489
           LA I+K S
Sbjct: 498 LATITKVS 503

BLAST of CcUC05G083850 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 5.8e-163
Identity = 273/486 (56.17%), Postives = 356/486 (73.25%), Query Frame = 0

Query: 6   AVAAVAAAAAVFAAVKWAAKSLNEWIYEAKLG-DRRMALPPGDLGWPIIGNMLGFLRAFK 65
           A  AV   A     V  AA+  + W  EA LG  RR  LPPG++GWP++G M  FLRAFK
Sbjct: 34  AAEAVLVGAVALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFK 93

Query: 66  SKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIEL 125
           S  P+ FI S+V RFG+ GVY+  +F +P+V+VTT E C++VL DD+AF  GWP+A + L
Sbjct: 94  SGKPDAFIASFVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVAL 153

Query: 126 IGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNM-GPIEFLT 185
           +G +SF+ MP +EH+R+R+LT+AP+NGF+AL+ Y+P+I+  V  SL  W++  G +EFLT
Sbjct: 154 VGPRSFVAMPYDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLT 213

Query: 186 QLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARKNLV 245
           +LR++TF II+ IFL    ++   ALE+ YT LNYG+RA+ IN+PGFAY  AL+AR+ LV
Sbjct: 214 ELRRMTFKIIVQIFLGGADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLV 273

Query: 246 AALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESS 305
           A LQ ++ +RR  R    +    DMMD LI+ +DE GR L D+EIID+LVMYLNAGHESS
Sbjct: 274 AVLQGVLDERRAARAKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESS 333

Query: 306 GHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRY 365
           GH  MWAT+ L ++P +  +AKAEQE I+R  P  Q+GLTL++ R+MEYLS+V+DETLR 
Sbjct: 334 GHITMWATVFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRL 393

Query: 366 VSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFIPKA 425
           V+ S V FR+A  DV +NGYLIPKGWKV  W+RS+H D +VYPDP KFDPSRW+G  P+A
Sbjct: 394 VNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRA 453

Query: 426 GEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAK 485
           G FL FG G+RLCPGNDLAKLEI +F+H+FLL YKL    P C + YLPH RP DNCLAK
Sbjct: 454 GTFLAFGLGARLCPGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAK 513

Query: 486 ISKNSS 490
           I++  S
Sbjct: 514 ITRVGS 519

BLAST of CcUC05G083850 vs. ExPASy TrEMBL
Match: A0A5D3CZZ0 (Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003480 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.1e-276
Identity = 473/497 (95.17%), Postives = 481/497 (96.78%), Query Frame = 0

Query: 1   MEVSW----AVAAVAAAAAV-FAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGN 60
           MEVSW    AV  VA AAAV FA VKWAAKS NEWIYEAKLG+RRMALPPGDLGWP+IGN
Sbjct: 2   MEVSWGTAAAVTVVAGAAAVFFAVVKWAAKSFNEWIYEAKLGERRMALPPGDLGWPLIGN 61

Query: 61  MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 120
           MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP
Sbjct: 62  MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 121

Query: 121 GWPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN 180
           GWPRAA+ELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS 
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWST 181

Query: 181 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRA 240
           MGPIEFLTQLRKLTFTIIMYIFLSAESESVME+LEKEYTRLNYGVRALRIN+PGFAYH+A
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKA 241

Query: 241 LKARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMY 300
           LKARKNLVAALQ IVT+RRKRRL KWAP RKDMMDALIDVEDENGRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301

Query: 301 LNAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSK 360
           LNAGHESSGHTMMWATILLNQHP+VLKKAK EQEEIVRRRPPGQKGLTLKECREMEYLSK
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSK 361

Query: 361 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSR 420
           VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421

Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 480
           WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 481

Query: 481 PKDNCLAKISKNSSTVV 493
           PKDNCLAKISKNSSTVV
Sbjct: 482 PKDNCLAKISKNSSTVV 498

BLAST of CcUC05G083850 vs. ExPASy TrEMBL
Match: A0A1S3AWI4 (ent-kaurenoic acid oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103483403 PE=3 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 9.1e-276
Identity = 472/496 (95.16%), Postives = 480/496 (96.77%), Query Frame = 0

Query: 1   MEVSW----AVAAVAAAAAV-FAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGN 60
           MEVSW    AV  VA AAAV FA VKWAAKS NEWIYEAKLG+RRMALPPGDLGWP+IGN
Sbjct: 2   MEVSWGTAAAVTVVAGAAAVFFAVVKWAAKSFNEWIYEAKLGERRMALPPGDLGWPLIGN 61

Query: 61  MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 120
           MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP
Sbjct: 62  MLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQP 121

Query: 121 GWPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN 180
           GWPRAA+ELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS 
Sbjct: 122 GWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWST 181

Query: 181 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRA 240
           MGPIEFLTQLRKLTFTIIMYIFLSAESESVME+LEKEYTRLNYGVRALRIN+PGFAYH+A
Sbjct: 182 MGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKA 241

Query: 241 LKARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMY 300
           LKARKNLVAALQ IVT+RRKRRL KWAP RKDMMDALIDVEDENGRKLTDEEIIDILVMY
Sbjct: 242 LKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMY 301

Query: 301 LNAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSK 360
           LNAGHESSGHTMMWATILLNQHP+VLKKAK EQEEIVRRRPPGQKGLTLKECREMEYLSK
Sbjct: 302 LNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSK 361

Query: 361 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSR 420
           VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYD EVYPDPKKFDPSR
Sbjct: 362 VVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSR 421

Query: 421 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 480
           WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR
Sbjct: 422 WDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSR 481

Query: 481 PKDNCLAKISKNSSTV 492
           PKDNCLAKISKNSSTV
Sbjct: 482 PKDNCLAKISKNSSTV 497

BLAST of CcUC05G083850 vs. ExPASy TrEMBL
Match: A0A6J1E9S4 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 8.2e-261
Identity = 436/493 (88.44%), Postives = 466/493 (94.52%), Query Frame = 0

Query: 1   MEVSWAVA----AVAAAAAVFAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNM 60
           ME +WAVA    AV A A VF+ +KWAAKSLNEWIYEAKLGDRR+ALPPGDLGWP+IGNM
Sbjct: 1   MESTWAVAAVVTAVVAVATVFSVLKWAAKSLNEWIYEAKLGDRRLALPPGDLGWPLIGNM 60

Query: 61  LGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120
           LGFLRAFKSKNPETFID YVSR+GK GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG
Sbjct: 61  LGFLRAFKSKNPETFIDGYVSRYGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120

Query: 121 WPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNM 180
           WPRAAIELIG+KSFI+MP EEHKRLRRLTSAPVNGFEALSNYIPYIE+NVL+SL+KWS M
Sbjct: 121 WPRAAIELIGEKSFIQMPEEEHKRLRRLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKM 180

Query: 181 GPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRAL 240
           GPIEFLTQLRKLTFT+IMYIFLS+ESE VME LEKEYTRLNYGVRA+RIN+PGFAYH+AL
Sbjct: 181 GPIEFLTQLRKLTFTVIMYIFLSSESEPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKAL 240

Query: 241 KARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYL 300
           KARKNLVAAL+ IVT+RR+++LDKWAP RKDMMD LID+EDENGRKL DEEIIDIL+MYL
Sbjct: 241 KARKNLVAALKGIVTERRRQKLDKWAPKRKDMMDQLIDIEDENGRKLDDEEIIDILIMYL 300

Query: 301 NAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKV 360
           NAGHESSGHTMMWATILLNQHP+VLKKA+ EQE IVR RP GQ GLTLKECR+MEYLSKV
Sbjct: 301 NAGHESSGHTMMWATILLNQHPEVLKKAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKV 360

Query: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRW 420
           VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRW
Sbjct: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRW 420

Query: 421 DGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRP 480
           DGF+PKAGEFLPFGAGSRLCPGNDLAKLEICIF+HYFLLNY LEWLTPDC ILYLPHSRP
Sbjct: 421 DGFVPKAGEFLPFGAGSRLCPGNDLAKLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRP 480

Query: 481 KDNCLAKISKNSS 490
           KDNC+AKI+K SS
Sbjct: 481 KDNCMAKITKKSS 493

BLAST of CcUC05G083850 vs. ExPASy TrEMBL
Match: A0A5A7U100 (Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G003090 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 4.1e-260
Identity = 438/452 (96.90%), Postives = 445/452 (98.45%), Query Frame = 0

Query: 41  MALPPGDLGWPIIGNMLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTP 100
           MALPPGDLGWP+IGNMLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTP
Sbjct: 1   MALPPGDLGWPLIGNMLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTP 60

Query: 101 ETCRKVLTDDEAFQPGWPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIP 160
           ETCRKVLTDDEAFQPGWPRAA+ELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIP
Sbjct: 61  ETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIP 120

Query: 161 YIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGV 220
           YIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIFLSAESESVME+LEKEYTRLNYGV
Sbjct: 121 YIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGV 180

Query: 221 RALRINIPGFAYHRALKARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENG 280
           RALRIN+PGFAYH+ALKARKNLVAALQ IVT+RRKRRL KWAP RKDMMDALIDVEDENG
Sbjct: 181 RALRINLPGFAYHKALKARKNLVAALQGIVTERRKRRLGKWAPKRKDMMDALIDVEDENG 240

Query: 281 RKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQK 340
           RKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHP+VLKKAK EQEEIVRRRPPGQK
Sbjct: 241 RKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPPGQK 300

Query: 341 GLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHY 400
           GLTLKECREMEYLSKVVDETLRYVSFSLV FREAQMDVNLNGYLIPKGWKVLAWFRSIHY
Sbjct: 301 GLTLKECREMEYLSKVVDETLRYVSFSLVAFREAQMDVNLNGYLIPKGWKVLAWFRSIHY 360

Query: 401 DSEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLE 460
           D EVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLE
Sbjct: 361 DDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLE 420

Query: 461 WLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 493
           WLTPDCPILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 421 WLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 452

BLAST of CcUC05G083850 vs. ExPASy TrEMBL
Match: A0A6J1IVT9 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.0e-259
Identity = 433/493 (87.83%), Postives = 465/493 (94.32%), Query Frame = 0

Query: 1   MEVSWAVA----AVAAAAAVFAAVKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNM 60
           ME +WAVA    AV A A VF+ +KWAAKSLNEWIYEAKLGDRR+ALPPGDLGWP+IGNM
Sbjct: 1   MESTWAVAAVVTAVVAVATVFSVLKWAAKSLNEWIYEAKLGDRRLALPPGDLGWPLIGNM 60

Query: 61  LGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120
           LGFLRAFKSKNPETFID YVSR+GK GVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG
Sbjct: 61  LGFLRAFKSKNPETFIDGYVSRYGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPG 120

Query: 121 WPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNM 180
           WPRAA+ELIG+KSFI+MP EEHKRLRRLTSAPVNGFEALSNYIPYIE+NVL+SL+KWS M
Sbjct: 121 WPRAAVELIGEKSFIQMPQEEHKRLRRLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKM 180

Query: 181 GPIEFLTQLRKLTFTIIMYIFLSAESESVMEALEKEYTRLNYGVRALRINIPGFAYHRAL 240
           GPIEFLTQLRKLTFT+IMYIFLS+ESE VME LEKEYTRLNYGVRA+RIN+PGFAYH+AL
Sbjct: 181 GPIEFLTQLRKLTFTVIMYIFLSSESEPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKAL 240

Query: 241 KARKNLVAALQAIVTDRRKRRLDKWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYL 300
           KARKNLVAAL+ IVT+RR+++LDKWAP RKDMMD LID+ DENGRKL DEEIIDIL+MYL
Sbjct: 241 KARKNLVAALKGIVTERRRQKLDKWAPKRKDMMDQLIDIVDENGRKLDDEEIIDILIMYL 300

Query: 301 NAGHESSGHTMMWATILLNQHPKVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKV 360
           NAGHESSGHTMMWATILLNQHP+VLKKA+ EQE IVR RP GQ GLTLKECR+MEYLSKV
Sbjct: 301 NAGHESSGHTMMWATILLNQHPEVLKKAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKV 360

Query: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRW 420
           VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKF+PSRW
Sbjct: 361 VDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFEPSRW 420

Query: 421 DGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRP 480
           DGF+PKAGEFLPFGAGSRLCPGNDLAKLEICIF+HYFLLNY LEWLTPDC ILYLPHSRP
Sbjct: 421 DGFVPKAGEFLPFGAGSRLCPGNDLAKLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRP 480

Query: 481 KDNCLAKISKNSS 490
           KDNC+AKI+K SS
Sbjct: 481 KDNCMAKITKKSS 493

BLAST of CcUC05G083850 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 696.8 bits (1797), Expect = 1.2e-200
Identity = 316/467 (67.67%), Postives = 394/467 (84.37%), Query Frame = 0

Query: 20  VKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNMLGFLRAFKSKNPETFIDSYVSRF 79
           + W  + +N W+YE+ LG+ R  LPPGDLGWP IGNML FLRAFK+ +P++F  + + R+
Sbjct: 22  LNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRY 81

Query: 80  GKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIELIGKKSFIEMPVEEHK 139
           G  G+YK H+FGNPS++VTT +TCR+VLTDD+AF+PGWP + +ELIG+KSF+ +  EEHK
Sbjct: 82  GPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHK 141

Query: 140 RLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLS 199
           RLRRLT+APVNG EALS YIPYIEENV+  LDKW+ MG  EFLT LRKLTF IIMYIFLS
Sbjct: 142 RLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIFLS 201

Query: 200 AESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARKNLVAALQAIVTDRRKRRLD 259
           +ESE+VM+ALE+EYT LNYGVRA+ +NIPGFAYHRALKARK LVAA Q+IVT+RR +R  
Sbjct: 202 SESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQ 261

Query: 260 KWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPK 319
                +KDM+D L++V+DE+G+ L DEEIID+L+MYLNAGHESSGHT+MWAT+ L +HP+
Sbjct: 262 NILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPE 321

Query: 320 VLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVN 379
           VL++AKAEQE I++ RP GQKGL+LKE R+ME+LS+VVDETLR ++FSL  FREA+ DV 
Sbjct: 322 VLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVE 381

Query: 380 LNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWD-GFIPKAGEFLPFGAGSRLCPG 439
           +NGYLIPKGWKVL WFR +H D EV+PDP+KFDP+RWD GF+PKAG FLPFGAGS LCPG
Sbjct: 382 MNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPG 441

Query: 440 NDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKIS 486
           NDLAKLEI IF+H+FLL Y+++   P+CP++YLPH+RP DNCLA+IS
Sbjct: 442 NDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488

BLAST of CcUC05G083850 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 691.4 bits (1783), Expect = 5.2e-199
Identity = 318/467 (68.09%), Postives = 392/467 (83.94%), Query Frame = 0

Query: 20  VKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNMLGFLRAFKSKNPETFIDSYVSRF 79
           +KW  K +N WIY +KLG+++  LPPGDLGWP+IGNM  FLRAFK+ +PE+FI SY++R+
Sbjct: 21  LKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFIQSYITRY 80

Query: 80  GKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIELIGKKSFIEMPVEEHK 139
           G+ G+YK H+FG P V+VTTPETCR+VLTDD+AF  GWP++ ++LIG+KSF+ +  EEHK
Sbjct: 81  GRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHK 140

Query: 140 RLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLS 199
           RLRRLTSAPVNG EALS YI +IEE V   L+KWS MG IEFL+ LRKLTF +IMYIFLS
Sbjct: 141 RLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLS 200

Query: 200 AESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARKNLVAALQAIVTDRRKRRLD 259
           +ESE VM++LE+EYT LNYGVRA+ IN+PGFAYHRALKARK LVAA Q+IVT+RR +R  
Sbjct: 201 SESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQ 260

Query: 260 KWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPK 319
             +  RKDM+D LIDV+DENGR L DEEIID+L+MYLNAGHESSGH  MWATIL+ +HP 
Sbjct: 261 NISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPM 320

Query: 320 VLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVN 379
           +L+KAK EQE IV++R PGQK LTLKE REM YLS+V+DETLR ++FSL  FREA+ DV 
Sbjct: 321 ILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQ 380

Query: 380 LNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGN 439
           ++GY+IPKGWKVL WFR++H D E+YPDPKKFDPSRW+G+ PKAG FLPFG GS LCPGN
Sbjct: 381 MDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGN 440

Query: 440 DLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISK 487
           DLAKLEI IF+H+FLL Y++E   P CP+++LPH+RPKDNCLA+I++
Sbjct: 441 DLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of CcUC05G083850 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 691.4 bits (1783), Expect = 5.2e-199
Identity = 318/467 (68.09%), Postives = 392/467 (83.94%), Query Frame = 0

Query: 20  VKWAAKSLNEWIYEAKLGDRRMALPPGDLGWPIIGNMLGFLRAFKSKNPETFIDSYVSRF 79
           +KW  K +N WIY +KLG+++  LPPGDLGWP+IGNM  FLRAFK+ +PE+FI SY++R+
Sbjct: 21  LKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFIQSYITRY 80

Query: 80  GKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIELIGKKSFIEMPVEEHK 139
           G+ G+YK H+FG P V+VTTPETCR+VLTDD+AF  GWP++ ++LIG+KSF+ +  EEHK
Sbjct: 81  GRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHK 140

Query: 140 RLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLS 199
           RLRRLTSAPVNG EALS YI +IEE V   L+KWS MG IEFL+ LRKLTF +IMYIFLS
Sbjct: 141 RLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLS 200

Query: 200 AESESVMEALEKEYTRLNYGVRALRINIPGFAYHRALKARKNLVAALQAIVTDRRKRRLD 259
           +ESE VM++LE+EYT LNYGVRA+ IN+PGFAYHRALKARK LVAA Q+IVT+RR +R  
Sbjct: 201 SESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQ 260

Query: 260 KWAPMRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPK 319
             +  RKDM+D LIDV+DENGR L DEEIID+L+MYLNAGHESSGH  MWATIL+ +HP 
Sbjct: 261 NISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPM 320

Query: 320 VLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVN 379
           +L+KAK EQE IV++R PGQK LTLKE REM YLS+V+DETLR ++FSL  FREA+ DV 
Sbjct: 321 ILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQ 380

Query: 380 LNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGN 439
           ++GY+IPKGWKVL WFR++H D E+YPDPKKFDPSRW+G+ PKAG FLPFG GS LCPGN
Sbjct: 381 MDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGN 440

Query: 440 DLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISK 487
           DLAKLEI IF+H+FLL Y++E   P CP+++LPH+RPKDNCLA+I++
Sbjct: 441 DLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of CcUC05G083850 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 280.4 bits (716), Expect = 2.8e-75
Identity = 162/460 (35.22%), Postives = 252/460 (54.78%), Query Frame = 0

Query: 31  IYEAKLGDRRMALPPGDLGWPIIGNMLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLF 90
           I   K  + R  LPPG +GWP +G  L       S+NP  F  S   R+G+I  +K  + 
Sbjct: 22  IVSKKKKNSRGKLPPGSMGWPYLGETL----QLYSQNPNVFFTSKQKRYGEI--FKTRIL 81

Query: 91  GNPSVVVTTPETCRKVL-TDDEAFQPGWPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPV 150
           G P V++ +PE  R VL T    F+P +PR+  +LIG  +      + H  +R+L  +  
Sbjct: 82  GYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSF 141

Query: 151 NGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTI-IMYIFLSAESESVMEA 210
              E +   IP IE   L SL  W+NM  +    +++K  F + I+ IF   ES S  E 
Sbjct: 142 YP-ETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHLES-SYKEI 201

Query: 211 LEKEYTRLNYGVRALRINIPGFAYHRALKARKNLVAALQAIVTDRRKRRLDKWAPMRKDM 270
           L+  Y  ++ G  +  +++PG +YH+AL ARK L   +  I+ +RR++R      ++ D 
Sbjct: 202 LKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKR-----ALQTDF 261

Query: 271 MDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPKVLKKAKAEQ 330
           +  L++ ++E GR LT E+I D ++  L A  +++   + W    L+   K+L+  KAEQ
Sbjct: 262 LGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQ 321

Query: 331 EEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPKG 390
           + I       +K LT ++ R M    KV+ E+LR  S     FREA +DV   GYLIPKG
Sbjct: 322 KAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKG 381

Query: 391 WKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICI 450
           WKV+  FR+IH++ + + +P+ FDPSR++   PK   F+PFG+G   CPGN+LAKL+I I
Sbjct: 382 WKVMPLFRNIHHNPKYFSNPEVFDPSRFE-VNPKPNTFMPFGSGVHACPGNELAKLQILI 441

Query: 451 FIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNS 489
           F+H+ + N++ E    +  I Y P   P++   A   ++S
Sbjct: 442 FLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATFRRHS 467

BLAST of CcUC05G083850 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 258.8 bits (660), Expect = 8.6e-69
Identity = 150/454 (33.04%), Postives = 241/454 (53.08%), Query Frame = 0

Query: 31  IYEAKLGDRRMALPPGDLGWPIIGNMLGFLRAFKSKNPETFIDSYVSRFGKIGVYKVHLF 90
           I + + G  ++ LPPG +GWP +G          S++P  F  S   R+G   V+K H+ 
Sbjct: 24  ISQRRFGSSKLPLPPGTMGWPYVGETF----QLYSQDPNVFFQSKQKRYG--SVFKTHVL 83

Query: 91  GNPSVVVTTPETCRKVL-TDDEAFQPGWPRAAIELIGKKSFIEMPVEEHKRLRRLTSAPV 150
           G P V++++PE  + VL T    F+P +P +   ++GK++      + H +LR+L    +
Sbjct: 84  GCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLV---L 143

Query: 151 NGF--EALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESESVME 210
             F  E++ N +P IE     SL  W     I    +++  TF + +      +     E
Sbjct: 144 RAFMPESIRNMVPDIESIAQDSLRSWEGT-MINTYQEMKTYTFNVALLSIFGKDEVLYRE 203

Query: 211 ALEKEYTRLNYGVRALRINIPGFAYHRALKARKNLVAALQAIVTDRRKRRLDKWAPMRKD 270
            L++ Y  L  G  ++ +N+PG  +H+++KARK L   L  I+++RR+           D
Sbjct: 204 DLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQN-----GSSHND 263

Query: 271 MMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPKVLKKAKAE 330
           ++ + +  ++E    LTDE+I D ++  + A  +++   M W    L ++P VL+    E
Sbjct: 264 LLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEE 323

Query: 331 QEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPK 390
           Q  I + +  G+  LT  + ++M   S+V+ ETLR  S     FREA  DV   GYLIPK
Sbjct: 324 QMAIRKDKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPK 383

Query: 391 GWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEIC 450
           GWKVL  FR+IH+ ++++ +P KFDPSR++   PK   F+PFG G+  CPGN+LAKLE+ 
Sbjct: 384 GWKVLPLFRNIHHSADIFSNPGKFDPSRFE-VAPKPNTFMPFGNGTHSCPGNELAKLEMS 443

Query: 451 IFIHYFLLNYKLEWLTPDCPILYLPHSR-PKDNC 481
           I IH+    Y+   L        + HSR PK +C
Sbjct: 444 IMIHHLTTKYRFGQLLERATGFSMGHSRFPKTDC 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902869.11.3e-27996.75ent-kaurenoic acid oxidase 1-like [Benincasa hispida][more]
TYK17483.16.4e-27695.17ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa][more]
XP_008438234.11.9e-27595.16PREDICTED: ent-kaurenoic acid oxidase 2-like [Cucumis melo][more]
XP_004133946.12.7e-27494.16ent-kaurenoic acid oxidase 1 isoform X1 [Cucumis sativus] >KAE8650281.1 hypothet... [more]
XP_022924644.11.7e-26088.44ent-kaurenoic acid oxidase 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O230511.7e-19967.67Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q9C5Y27.3e-19868.09Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
Q9AXH92.2e-17058.33Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1[more]
Q5VRM73.4e-16354.92Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Q432465.8e-16356.17Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CZZ03.1e-27695.17Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3AWI49.1e-27695.16ent-kaurenoic acid oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103483403 PE=3 S... [more]
A0A6J1E9S48.2e-26188.44ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 ... [more]
A0A5A7U1004.1e-26096.90Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A6J1IVT92.0e-25987.83ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE... [more]
Match NameE-valueIdentityDescription
AT1G05160.11.2e-20067.67cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT2G32440.15.2e-19968.09ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.25.2e-19968.09ent-kaurenoic acid hydroxylase 2 [more]
AT3G19270.12.8e-7535.22cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT4G19230.28.6e-6933.04cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 394..418
score: 26.08
coord: 436..459
score: 29.77
coord: 307..333
score: 25.62
coord: 426..436
score: 57.74
IPR001128Cytochrome P450PRINTSPR00385P450coord: 427..436
score: 59.89
coord: 298..315
score: 27.64
coord: 355..366
score: 33.11
coord: 436..447
score: 38.98
IPR001128Cytochrome P450PFAMPF00067p450coord: 44..462
e-value: 9.5E-67
score: 225.7
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 34..487
e-value: 1.4E-107
score: 362.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 39..484
NoneNo IPR availablePANTHERPTHR24286:SF289ENT-KAURENOIC ACID OXIDASE 2coord: 15..480
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 15..480
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 429..438

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC05G083850.1CcUC05G083850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051777 ent-kaurenoate oxidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen