CcUC04G077750 (gene) Watermelon (PI 537277) v1

BLAST of CcUC04G077750 vs. NCBI nr
Match: XP_038881614.1 (probable ubiquitin-conjugating enzyme E2 24 [Benincasa hispida])

HSP 1 Score: 1736.9 bits (4497), Expect = 0.0e+00
Identity = 843/910 (92.64%), Postives = 874/910 (96.04%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSK+LLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKNLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDGT CEI  VDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGTKCEITTVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHANW 240
           LSGWKDDRCEGTVC VEAGLVYVDWLAS+L+GCSSSL APP +QHAKEL  LSCFPHANW
Sbjct: 181 LSGWKDDRCEGTVCYVEAGLVYVDWLASVLMGCSSSLMAPPRLQHAKELISLSCFPHANW 240

Query: 241 NLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFPVDEK++YFGEK ITTHDL NGGYRGTG QEICVIVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPVDEKDNYFGEKLITTHDLINGGYRGTGFQEICVIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQLR 360
           T LDSHCLTPAN +NAHEFWPQQFVMEKGNCDDSSMS+ QRWGVVQAVDAKERTVKVQ+R
Sbjct: 301 TGLDSHCLTPANVMNAHEFWPQQFVMEKGNCDDSSMSVYQRWGVVQAVDAKERTVKVQMR 360

Query: 361 TGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEIS 420
           T D SEGSNLDGL+VEETMSAYEL+EHPDY+FSLGD+VFRLALDKFGSQ D+NNI+ EIS
Sbjct: 361 TDDLSEGSNLDGLLVEETMSAYELVEHPDYNFSLGDVVFRLALDKFGSQSDKNNIISEIS 420

Query: 421 MNESAQNGENHIIDNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDKY 480
           MNESAQNG+NHI+DNNMN +NFLSFIG+VTGFKDGAVEVKWANCSTSMVSPQEIFPIDKY
Sbjct: 421 MNESAQNGKNHIVDNNMNMDNFLSFIGHVTGFKDGAVEVKWANCSTSMVSPQEIFPIDKY 480

Query: 481 EVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAIEF 540
           E+ST A  NGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAG+NFKRDTTCFFSRAAIEF
Sbjct: 481 EISTTAEPNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGKNFKRDTTCFFSRAAIEF 540

Query: 541 FSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLHNF 600
           FSNIF+T FGSVRSTI+SGTFTALQSFEGEDEIDLLCEKEVLQTCS+S EMSQ+AK HNF
Sbjct: 541 FSNIFSTFFGSVRSTIISGTFTALQSFEGEDEIDLLCEKEVLQTCSESCEMSQIAKSHNF 600

Query: 601 GETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWLK 660
           GETS +IEEIHK +DLT STSSENYDLF QFDMASDCPDHHFLSSGKGS+LSQASRGWLK
Sbjct: 601 GETS-NIEEIHKYEDLTLSTSSENYDLFAQFDMASDCPDHHFLSSGKGSILSQASRGWLK 660

Query: 661 KVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIP 720
           KVQQEWSIL+NNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIP
Sbjct: 661 KVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIP 720

Query: 721 PLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK 780
           PLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVW+PGSSTILQ+LLSLQALVLNEK
Sbjct: 721 PLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWSPGSSTILQILLSLQALVLNEK 780

Query: 781 PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQ 840
           PYYNEAGYDAQLGR EGERNSASYNENAFLVTCKSMMYLLSKPPKHFE LVEEHFRRRYQ
Sbjct: 781 PYYNEAGYDAQLGRTEGERNSASYNENAFLVTCKSMMYLLSKPPKHFETLVEEHFRRRYQ 840

Query: 841 HILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCSQ 900
           HILAACRAYMEGTPVGSDYH G TKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCSQ
Sbjct: 841 HILAACRAYMEGTPVGSDYHRGTTKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCSQ 900

Query: 901 FAEAENVLIL 911
           F E EN LIL
Sbjct: 901 FVEVENGLIL 909

BLAST of CcUC04G077750 vs. NCBI nr
Match: XP_008439854.1 (PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >XP_008439855.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >XP_008439856.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >XP_008439858.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >KAA0052687.1 putative ubiquitin-conjugating enzyme E2 24 [Cucumis melo var. makuwa] >TYK13137.1 putative ubiquitin-conjugating enzyme E2 24 [Cucumis melo var. makuwa])

HSP 1 Score: 1725.7 bits (4468), Expect = 0.0e+00
Identity = 847/911 (92.97%), Postives = 871/911 (95.61%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVC VNDP+GQMGKVVGI+MLVDLEKN GPLIK+VNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDG  CEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLASIL+GCSSSLTAPP MQHAKELTLLSCF HANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 NLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFP DEKN+YFGEKF +THDL NGGYRGTG  EIC+IVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQLR 360
           TALDS CLTPANA NAHEFWPQQFVMEKGNCDDSSMS+ QRWGVVQAVDAKERTVKVQLR
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEIS 420
           T D SEGS+LDGL+VEETMSAYEL+EHPDYSFSLGDIVFRLA+DKF SQ DENNI+ EIS
Sbjct: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420

Query: 421 MNESAQNGENHII-DNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MNESAQN  NHII +NNMN +NFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK
Sbjct: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAIE 540
           YEVS+IAV NGENLEESR +QIEPLKPYNIQKGKDMPNCDGA +NFKRDTTCFFSRAA+E
Sbjct: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLHN 600
           FFSNIFTTLFGSVRSTILSGTFTALQSFEG DEIDLLCEKEVLQTCSQSTEMSQ+AKLHN
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWL 660
           FGETSL IEEIHKN+DLTFSTSS+NYDL TQFDMASDCPDHHFLSSGKGSMLSQASR WL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660

Query: 661 KKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720
           KKVQQEWSIL+NNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSK PKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCS 900
           QHILAACRAYMEGTPVGSDY  G TK ENPNQSSTGFKIMLAKIFPRLIKTFSSKG DCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

Query: 901 QFAEAENVLIL 911
           QF EAEN  IL
Sbjct: 901 QFMEAENGFIL 911

BLAST of CcUC04G077750 vs. NCBI nr
Match: XP_004134704.1 (probable ubiquitin-conjugating enzyme E2 24 [Cucumis sativus] >XP_031743225.1 probable ubiquitin-conjugating enzyme E2 24 [Cucumis sativus] >XP_031743226.1 probable ubiquitin-conjugating enzyme E2 24 [Cucumis sativus])

HSP 1 Score: 1721.1 bits (4456), Expect = 0.0e+00
Identity = 844/910 (92.75%), Postives = 867/910 (95.27%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVC VNDP+GQMGKVVGIDMLVDLEKN GP+IK+VNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIDMLVDLEKNSGPIIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDG  CEIIAVDQEKLLPISVD LDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDVLDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLAS+L+GCSSSLTAPP MQHAKELTLLSCF HANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 NLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFP DEKN+YFGEKFITTHDL NGGYRGTG Q+ICVIVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFITTHDLMNGGYRGTGFQDICVIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQLR 360
           TALDS CLTPANA NAHEFWPQQFVMEKGNCDDSSMS+ QRWGVVQAVDAKERTVKVQLR
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEIS 420
           T D SEGSNLDGL+VEETMSAYEL+EHPDYSFSLGDIVFRLALDKF SQ DENNI+ EIS
Sbjct: 361 TDDLSEGSNLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFASQSDENNIISEIS 420

Query: 421 MNESAQNGENHIIDNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDKY 480
           MNESAQN  N II+NNM  +NFLSFIGNVTGFKDGAVEVKWANCS SMVSPQEIF IDKY
Sbjct: 421 MNESAQNEGNRIINNNMIMDNFLSFIGNVTGFKDGAVEVKWANCSISMVSPQEIFSIDKY 480

Query: 481 EVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAIEF 540
           EVS IAV NGENLEESR +QIEPLKPYNIQKGKDMPNCDGA +NFKRDTTCFFSRAA+EF
Sbjct: 481 EVSPIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVEF 540

Query: 541 FSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLHNF 600
           FSNIFTTLFGSVRSTILSGTFTALQSFEG DEI+LLCEKEVLQTCSQSTEMSQ+AKLHNF
Sbjct: 541 FSNIFTTLFGSVRSTILSGTFTALQSFEGGDEINLLCEKEVLQTCSQSTEMSQIAKLHNF 600

Query: 601 GETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWLK 660
           GETSL IEEIHKN+DLTFSTSSENYDL TQFDMASDCPDHHFLSSGKG MLSQASR WLK
Sbjct: 601 GETSLDIEEIHKNEDLTFSTSSENYDLLTQFDMASDCPDHHFLSSGKGPMLSQASRRWLK 660

Query: 661 KVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIP 720
           KVQQEWSIL+NNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQIP
Sbjct: 661 KVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQIP 720

Query: 721 PLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK 780
           PLVHYISGGLRVNPNLYESG+VCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK
Sbjct: 721 PLVHYISGGLRVNPNLYESGRVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK 780

Query: 781 PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQ 840
           PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSK PKHFE LVEEHFRRRYQ
Sbjct: 781 PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFEALVEEHFRRRYQ 840

Query: 841 HILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCSQ 900
           HILAACRAYMEGTPVGSDY  G TK ENP+QSSTGFKIMLAKIFPRLIKTFSSKG DCSQ
Sbjct: 841 HILAACRAYMEGTPVGSDYQCGTTKPENPDQSSTGFKIMLAKIFPRLIKTFSSKGFDCSQ 900

Query: 901 FAEAENVLIL 911
           F EAEN LIL
Sbjct: 901 FMEAENGLIL 910

BLAST of CcUC04G077750 vs. NCBI nr
Match: KAE8647689.1 (hypothetical protein Csa_003794 [Cucumis sativus])

HSP 1 Score: 1721.1 bits (4456), Expect = 0.0e+00
Identity = 844/910 (92.75%), Postives = 867/910 (95.27%), Query Frame = 0

Query: 1    MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
            MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 440  MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 499

Query: 61   AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
            AVC VNDP+GQMGKVVGIDMLVDLEKN GP+IK+VNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 500  AVCFVNDPYGQMGKVVGIDMLVDLEKNSGPIIKNVNSKDLLKIRSISAGDVVVNGPWIGR 559

Query: 121  VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
            VDKVIDSVSVLFDDG  CEIIAVDQEKLLPISVD LDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 560  VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDVLDDSQYPYYPGQRVRFSNVSKPPGW 619

Query: 181  LSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHANW 240
            LSGWKDDRCEGTVCSVEAGLVYVDWLAS+L+GCSSSLTAPP MQHAKELTLLSCF HANW
Sbjct: 620  LSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFSHANW 679

Query: 241  NLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSRS 300
            NLGDWCIFP DEKN+YFGEKFITTHDL NGGYRGTG Q+ICVIVKKKTKVDVLWQDGSRS
Sbjct: 680  NLGDWCIFPGDEKNNYFGEKFITTHDLMNGGYRGTGFQDICVIVKKKTKVDVLWQDGSRS 739

Query: 301  TALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQLR 360
            TALDS CLTPANA NAHEFWPQQFVMEKGNCDDSSMS+ QRWGVVQAVDAKERTVKVQLR
Sbjct: 740  TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 799

Query: 361  TGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEIS 420
            T D SEGSNLDGL+VEETMSAYEL+EHPDYSFSLGDIVFRLALDKF SQ DENNI+ EIS
Sbjct: 800  TDDLSEGSNLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFASQSDENNIISEIS 859

Query: 421  MNESAQNGENHIIDNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDKY 480
            MNESAQN  N II+NNM  +NFLSFIGNVTGFKDGAVEVKWANCS SMVSPQEIF IDKY
Sbjct: 860  MNESAQNEGNRIINNNMIMDNFLSFIGNVTGFKDGAVEVKWANCSISMVSPQEIFSIDKY 919

Query: 481  EVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAIEF 540
            EVS IAV NGENLEESR +QIEPLKPYNIQKGKDMPNCDGA +NFKRDTTCFFSRAA+EF
Sbjct: 920  EVSPIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVEF 979

Query: 541  FSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLHNF 600
            FSNIFTTLFGSVRSTILSGTFTALQSFEG DEI+LLCEKEVLQTCSQSTEMSQ+AKLHNF
Sbjct: 980  FSNIFTTLFGSVRSTILSGTFTALQSFEGGDEINLLCEKEVLQTCSQSTEMSQIAKLHNF 1039

Query: 601  GETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWLK 660
            GETSL IEEIHKN+DLTFSTSSENYDL TQFDMASDCPDHHFLSSGKG MLSQASR WLK
Sbjct: 1040 GETSLDIEEIHKNEDLTFSTSSENYDLLTQFDMASDCPDHHFLSSGKGPMLSQASRRWLK 1099

Query: 661  KVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIP 720
            KVQQEWSIL+NNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQIP
Sbjct: 1100 KVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQIP 1159

Query: 721  PLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK 780
            PLVHYISGGLRVNPNLYESG+VCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK
Sbjct: 1160 PLVHYISGGLRVNPNLYESGRVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK 1219

Query: 781  PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQ 840
            PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSK PKHFE LVEEHFRRRYQ
Sbjct: 1220 PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFEALVEEHFRRRYQ 1279

Query: 841  HILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCSQ 900
            HILAACRAYMEGTPVGSDY  G TK ENP+QSSTGFKIMLAKIFPRLIKTFSSKG DCSQ
Sbjct: 1280 HILAACRAYMEGTPVGSDYQCGTTKPENPDQSSTGFKIMLAKIFPRLIKTFSSKGFDCSQ 1339

Query: 901  FAEAENVLIL 911
            F EAEN LIL
Sbjct: 1340 FMEAENGLIL 1349

BLAST of CcUC04G077750 vs. NCBI nr
Match: XP_022978639.1 (probable ubiquitin-conjugating enzyme E2 24 [Cucurbita maxima] >XP_022978640.1 probable ubiquitin-conjugating enzyme E2 24 [Cucurbita maxima] >XP_022978641.1 probable ubiquitin-conjugating enzyme E2 24 [Cucurbita maxima])

HSP 1 Score: 1642.9 bits (4253), Expect = 0.0e+00
Identity = 807/905 (89.17%), Postives = 843/905 (93.15%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDL LSDSDWENSS+CDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 1   MDLILSDSDWENSSDCDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
            VCSVNDPFGQMGKVVGIDMLVDLE NHGP+IKDVNSKDL KIR IS GDVVV+G WIGR
Sbjct: 61  VVCSVNDPFGQMGKVVGIDMLVDLETNHGPIIKDVNSKDLRKIRPISVGDVVVHGKWIGR 120

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           V+KVIDSVSVLFDDGT CEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSN SKPP W
Sbjct: 121 VEKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNASKPPRW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHANW 240
           LSGWKD+RCEGTVCSV+AGLVYVDWLASIL+GCSSSLTAPP MQHAKELT+LSCFPHANW
Sbjct: 181 LSGWKDNRCEGTVCSVDAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTVLSCFPHANW 240

Query: 241 NLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSRS 300
           +LGDWCIFPVDEK+DY GEKFIT HDL  GGYR TGLQEI VIVKKKTKVDVLWQDGS S
Sbjct: 241 HLGDWCIFPVDEKSDYLGEKFITAHDLLTGGYRDTGLQEISVIVKKKTKVDVLWQDGSHS 300

Query: 301 TALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQLR 360
             LDSHCLTPANA+NAHEFWPQQFVMEKGNCDDSSMS TQRWGVVQAVDAKERTVKVQLR
Sbjct: 301 RGLDSHCLTPANAMNAHEFWPQQFVMEKGNCDDSSMSETQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEIS 420
           TGD +EGS+LDGL VEETMSAYEL+EHPDYSF+LGDIVFRLA   FGSQ DENNI  EIS
Sbjct: 361 TGDLTEGSDLDGLWVEETMSAYELVEHPDYSFTLGDIVFRLA--SFGSQSDENNITSEIS 420

Query: 421 MN-ESAQNGENHIIDNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MN ESAQNGE+HII NNMN +NFLSFIGNVT FKDG VEVKWANCS+SMVSP EIFPIDK
Sbjct: 421 MNEESAQNGESHIISNNMNMDNFLSFIGNVTSFKDGTVEVKWANCSSSMVSPHEIFPIDK 480

Query: 481 YEVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAIE 540
           YEVS IAV +G+N+EE RQDQIEP KP NIQKGKDMPN D AG+NF+RDTTCF SRAAIE
Sbjct: 481 YEVSAIAVPSGDNVEELRQDQIEPSKPDNIQKGKDMPNFDAAGKNFRRDTTCFLSRAAIE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLHN 600
           FFSN+FTTLFGSVRSTI+SGTFTALQSFEG D ID+L EKEVLQTCSQ T  SQ+AKL +
Sbjct: 541 FFSNVFTTLFGSVRSTIISGTFTALQSFEGGDNIDILPEKEVLQTCSQFTGRSQIAKLKS 600

Query: 601 FGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWL 660
           FGETSL+ EEIH+N+  T STSSENYDLFTQFDMASDCPDHHFLSSG  SMLSQA RGWL
Sbjct: 601 FGETSLNTEEIHENEASTLSTSSENYDLFTQFDMASDCPDHHFLSSGNASMLSQARRGWL 660

Query: 661 KKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720
           KKVQQEWSIL+NNLPETVYVRVFEERMDLIR+VIIGAPGTPYHDGLFFFDIFLPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRSVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCS 900
           QHILAACRAYMEGTPVG+DYH GA KL NPNQSSTGFKIMLAKI+PRLIKTFSSKGIDCS
Sbjct: 841 QHILAACRAYMEGTPVGADYHSGAKKLVNPNQSSTGFKIMLAKIYPRLIKTFSSKGIDCS 900

Query: 901 QFAEA 905
           QF EA
Sbjct: 901 QFFEA 903

BLAST of CcUC04G077750 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 1.7e-248
Identity = 455/920 (49.46%), Postives = 609/920 (66.20%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           M++ L+DSDW++SS+  SS+  E    Y G+   I S+LEETIGKIDEFLSFER F+ GD
Sbjct: 1   MEMSLTDSDWDSSSDSGSSEHEEVEFSYGGRAQNIFSNLEETIGKIDEFLSFERGFMYGD 60

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
            V S  +P GQ G+V+ IDM V+LE  HG ++K+V++K L K+RSIS  D V+NGPW+GR
Sbjct: 61  IVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRLQKLRSISLSDYVINGPWVGR 120

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQR--VRFSNVSKPP 180
           VDK+++ VSV  DDGTN E++   Q+KL+ I  + L+DSQY YYPGQR  V+ ++  +  
Sbjct: 121 VDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQYSYYPGQRVQVKLAHAPRST 180

Query: 181 GWLSG-WKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPH 240
            WL G W+  +  GTVC+VEAGLVYVDW+ASI++    +LTAP  +Q+ + LTLL C  H
Sbjct: 181 TWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNLTAPQALQNPESLTLLPCVSH 240

Query: 241 ANWNLGDWCIFPVDEKNDY------------FGEKFITTHDLTNGGYRGTGLQEICVIVK 300
           A+W LGDWCI P     D               E   T     N   + +GL E+ VI K
Sbjct: 241 ASWQLGDWCILPGSSHCDIAERQTPNVAAYNLNECHKTFQKGFNRNMQNSGLDELFVITK 300

Query: 301 KKTKVDVLWQDGSRSTALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVV 360
            K KV V+WQDGS S  +DS  L P  AVNAH+FWP+QFV+EK  C+      +++WGVV
Sbjct: 301 TKMKVAVMWQDGSCSLGVDSQQLLPVGAVNAHDFWPEQFVVEKETCN------SKKWGVV 360

Query: 361 QAVDAKERTVKVQLRTGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDK 420
           +AV+AKE+TVKVQ       E +     V+EE +SAYEL+EHPD+ F   D+V +L L +
Sbjct: 361 KAVNAKEQTVKVQWTIQVEKEATGCVDEVMEEIVSAYELLEHPDFGFCFSDVVVKL-LPE 420

Query: 421 FGSQPDENNIMYEISMNESAQNGENHIIDNNMNAENFLSFIGNVTGFKDGAVEVKWANCS 480
               P+ + I+        A   ++ + +++ +   FLS IG VTGFK+G+V+VKWAN S
Sbjct: 421 GKFDPNADTIV--------ATEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWANGS 480

Query: 481 TSMVSPQEIFPIDKYEVS-TIAVSNGENLEESRQ--DQIEPLKPYNIQKG--KDMPNCDG 540
           TS V+P EI+ +++ E S +  VS+  ++++  Q   Q +     + + G  K     + 
Sbjct: 481 TSKVAPCEIWKMERSEYSNSSTVSSEGSVQDLSQKISQSDEASSNHQETGLVKLYSVGES 540

Query: 541 AGQNFKRDTTCFFSRAAIEFFSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKE 600
             +N    ++ F  +AAI F +N+ ++LFG   ST +  + +     E + + ++L ++ 
Sbjct: 541 CNENIPECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQSDSEVLVQET 600

Query: 601 VLQTCSQSTEMSQVAKLHNFGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDH 660
                +  T   +V          +  + I+K  D T   +S N   F QFDM +DC DH
Sbjct: 601 AESYDNSETNSGEVDMTTTMVNIPIEGKGINKTLDSTLLENSRNQVRFRQFDMVNDCSDH 660

Query: 661 HFLSSGKGSMLSQASRGWLKKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTP 720
           HFLSS KG   SQ ++ W+KKVQQEWS L  NLP T+YVRV EERMDL+RA ++GAPGTP
Sbjct: 661 HFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPGTP 720

Query: 721 YHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNP 780
           YHDGLFFFDI LPP+YP  PP+VHY SGG+R+NPNLYESG+VCLSLLNTW+GSG+EVWN 
Sbjct: 721 YHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLLNTWSGSGTEVWNA 780

Query: 781 GSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLL 840
           GSS+ILQ+LLS QALVLNEKPY+NEAGYD QLGRAEGE+NS SYNENAFL+TCKSM+ +L
Sbjct: 781 GSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENAFLITCKSMISML 840

Query: 841 SKPPKHFEKLVEEHFRRRYQHILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIML 900
            KPPKHFE LV++HF  R QH+LAAC+AYMEG PVGS     A    N   +STGFKIML
Sbjct: 841 RKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS----SANLQGNSTTNSTGFKIML 900

BLAST of CcUC04G077750 vs. ExPASy Swiss-Prot
Match: Q9ZVX1 (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 1.5e-172
Identity = 372/985 (37.77%), Postives = 533/985 (54.11%), Query Frame = 0

Query: 41   ETIGKIDEFLSFEREFLLGDAVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDL 100
            E +  I++    +R FL GD V S ++P GQ+G VV +++ VDL    G + KD+++K+L
Sbjct: 130  EPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAPDGSIHKDISTKNL 189

Query: 101  LKIRSISAGDVVVNGPWIGRVDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDAL-DDS 160
             ++R  + GD VV+GPW+GR+D V+D+V+VLFDDG+ C+++ V+  +L PI  + L +D+
Sbjct: 190  KRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLRLKPIPKNNLEEDA 249

Query: 161  QYPYYPGQRVR--FSNVSKPPGWLSG-WKDDRCEGTVCSVEAGLVYVDWLASILIGCSSS 220
             +PYYPGQRV+   S+V K   WLSG WK +R EGTV  V AG ++V W+AS   G  SS
Sbjct: 250  NFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFVYWIASAGFGPDSS 309

Query: 221  LTAPPHMQHAKELTLLSCFPHANWNLGDWCIFP----------------------VDEKN 280
            + +PP  Q+   LTLLSCF HANW +GDWC+ P                        ++ 
Sbjct: 310  V-SPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVSKLRLYDSQADRQ 369

Query: 281  DYFGEKFITTHDLTNGGYRGTGL------------------------------------- 340
               G       D  +G     G+                                     
Sbjct: 370  QKIGRDLEDVQDEVSGKVEPAGITAEALPKVTSDDPPQRNPSVSKEPVHEPWPLHRKKIR 429

Query: 341  -----------------QEICVIVKKKTKVDVLWQDGSRSTALDSHCLTPANAVNAHEFW 400
                             ++  ++V  +T+VDV WQDG+     ++  L P      HEF 
Sbjct: 430  KLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQDGTIECRREAITLIPIETPGDHEFV 489

Query: 401  PQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQ----LRTGDSSEGSNLDGLVVE 460
             +Q+V+EK + D  + +  +R GVV+ V+AK+RT  V+    LR  +            E
Sbjct: 490  SEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTASVRWLNPLRRAEEPR-----EFEKE 549

Query: 461  ETMSAYELIEHPDYSFSLGDIVFRL---ALDKFGSQPDENNIMYEISMNESAQNGENH-- 520
            E +S YEL  HPDY +  GD+V RL   A+    S P  N+       +   Q+ E+H  
Sbjct: 550  EIVSVYELEGHPDYDYCYGDVVVRLSPIAVALPASSPG-NSFEEATQQDNGYQDSESHQE 609

Query: 521  ---IIDNNMNAENF----LSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK----- 580
               ++D   N  +     LS++GN+TG KDG +EV WA+ + S V P  ++ + +     
Sbjct: 610  AKILVDKEENEPSTDLSKLSWVGNITGLKDGDIEVTWADGTISTVGPHAVYVVGRDDDDE 669

Query: 581  ---YEVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDG---AGQNFKRDTTCFF 640
                E  T   ++ E L +  +   E  +    +      N D    A  +  R+     
Sbjct: 670  SVAGESETSDAASWETLNDDDRGAPEIPEEDLGRSSSIEGNSDADIYAENDSGRNGALAL 729

Query: 641  SRAAIEFFSNIFTTLFGSVRSTI--------LSGTFTALQSFEGEDEIDLL-----CEKE 700
              AAIEF + + + +F   R ++        +   +   +S    DE D L      +  
Sbjct: 730  PLAAIEFVTRLASGIFSRARKSVDSSSSDYTVENVYKQAESTNPSDETDSLDDPSPSKVN 789

Query: 701  VLQTC-SQSTEMSQVAKLHNFGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPD 760
            V   C S+ T+ +  AK    GETS  +E+    +D    +  ++   F +FD++ D  D
Sbjct: 790  VTDNCESKGTQAN--AKNILSGETSTFLED----EDKPVPSEGDSCS-FRRFDISQDPLD 849

Query: 761  HHFLS-SGKGSMLSQASRGWLKKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPG 820
            HHFL   G+        R W KKV Q+W IL+NNLP+ ++VR +E+RMDL+RAVI+GA G
Sbjct: 850  HHFLGVDGQ----KTKERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFG 909

Query: 821  TPYHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVW 880
            TPY DGLFFFD  LP +YP +PP  +Y SGG R+NPNLYE GKVCLSLLNTWTG G+EVW
Sbjct: 910  TPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVW 969

Query: 881  NPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMY 902
            +P SS+ILQVL+SLQ LVLN KPY+NEAGYD Q+G AEGE+NS  YNEN FL+ CK+MMY
Sbjct: 970  DPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMY 1029

BLAST of CcUC04G077750 vs. ExPASy Swiss-Prot
Match: Q8GY87 (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 297.4 bits (760), Expect = 5.7e-79
Identity = 145/270 (53.70%), Postives = 178/270 (65.93%), Query Frame = 0

Query: 628 FTQFDMASDCPDHHFLSSGKGSMLSQASRGWLKKVQQEWSILRNNLPETVYVRVFEERMD 687
           F +FD   D  DHH+ S GK S   Q S+ W+KKVQ +W IL N+LPE + VR  E RMD
Sbjct: 244 FKRFDTVEDFSDHHYASKGKSS--KQHSKNWVKKVQADWKILENDLPEAISVRACESRMD 303

Query: 688 LIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLL 747
           L+RAVIIGA GTPYHDGLFFFDI  P  YP +PP VHY SGGLR+NPNLY  GKVCLSLL
Sbjct: 304 LLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLL 363

Query: 748 NTWTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNEN 807
            TW GS  E W P  ST+LQ+L+S+QAL+LNEKPY+NE GY    G A GE  S  Y+EN
Sbjct: 364 GTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSEN 423

Query: 808 AFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQHILAACRAYMEGTPVGSDYHFGATKLE 867
            FL++ K+M+Y + +PP+HFE+ V+ H+  R   I+ AC AY  G P+GS    G   LE
Sbjct: 424 VFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLE 483

Query: 868 NPNQS-STGFKIMLAKIFPRLIKTFSSKGI 897
              QS S  FK  +A     ++  F   G+
Sbjct: 484 EARQSGSKKFKTDVASFMQTVVDEFVKLGV 511

BLAST of CcUC04G077750 vs. ExPASy Swiss-Prot
Match: Q9C918 (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 293.5 bits (750), Expect = 8.2e-78
Identity = 145/270 (53.70%), Postives = 179/270 (66.30%), Query Frame = 0

Query: 628 FTQFDMASDCPDHHFLSSGKGSMLSQASRGWLKKVQQEWSILRNNLPETVYVRVFEERMD 687
           F +FD   D  DHH++S GK S   Q S+ W+KKVQ EW IL  NLPET++VR  E RMD
Sbjct: 27  FKRFDSVEDFSDHHYVSKGKAS--KQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMD 86

Query: 688 LIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLL 747
           L+RAVIIGA GTPYHDGLFFFDI  P  YP +PP V+Y SGGLR+NPNLY  GKVCLSLL
Sbjct: 87  LLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCGKVCLSLL 146

Query: 748 NTWTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNEN 807
            TW G+  + W P  ST+LQVL+S+QALVLNE+PY+NE GY    G   GE  S  Y+EN
Sbjct: 147 GTWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSEN 206

Query: 808 AFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQHILAACRAYMEGTPVGSDYHFGATKLE 867
            FL++ K+M+Y + KPP+HFE+ V+ H+  R   I+ AC AY  G P+GS    G   LE
Sbjct: 207 VFLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLE 266

Query: 868 NPNQS-STGFKIMLAKIFPRLIKTFSSKGI 897
              QS S  FK  +A     ++  F   G+
Sbjct: 267 QARQSGSKKFKTDVASFMQTVVDEFVKLGV 294

BLAST of CcUC04G077750 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.6e-73
Identity = 127/232 (54.74%), Postives = 162/232 (69.83%), Query Frame = 0

Query: 625 YDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWLKKVQQEWSILRNNLPETVYVRVFEE 684
           Y  F +FD+  D  DHH+ S  KG+   Q S+ W K++Q EW IL  +LPE ++VR +E 
Sbjct: 302 YLFFKRFDIVEDFSDHHYAS--KGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYES 361

Query: 685 RMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCL 744
           RMDL+RAVIIGA GTPYHDGLFFFDIF P  YP  PP+VHY SGGLR+NPNLY  GKVCL
Sbjct: 362 RMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCL 421

Query: 745 SLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASY 804
           SLL TW+G+  E W P +ST+LQVL+S+Q L+LN+KPY+NE GY+   G A GE  S +Y
Sbjct: 422 SLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAY 481

Query: 805 NENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQHILAACRAYMEGTPVG 857
           +EN F+++ K+M+Y + +PPK+FE     HF      +L AC AY  G   G
Sbjct: 482 SENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPG 531

BLAST of CcUC04G077750 vs. ExPASy TrEMBL
Match: A0A1S3B0C7 (probable ubiquitin-conjugating enzyme E2 24 OS=Cucumis melo OX=3656 GN=LOC103484521 PE=4 SV=1)

HSP 1 Score: 1725.7 bits (4468), Expect = 0.0e+00
Identity = 847/911 (92.97%), Postives = 871/911 (95.61%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVC VNDP+GQMGKVVGI+MLVDLEKN GPLIK+VNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDG  CEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLASIL+GCSSSLTAPP MQHAKELTLLSCF HANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 NLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFP DEKN+YFGEKF +THDL NGGYRGTG  EIC+IVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQLR 360
           TALDS CLTPANA NAHEFWPQQFVMEKGNCDDSSMS+ QRWGVVQAVDAKERTVKVQLR
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEIS 420
           T D SEGS+LDGL+VEETMSAYEL+EHPDYSFSLGDIVFRLA+DKF SQ DENNI+ EIS
Sbjct: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420

Query: 421 MNESAQNGENHII-DNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MNESAQN  NHII +NNMN +NFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK
Sbjct: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAIE 540
           YEVS+IAV NGENLEESR +QIEPLKPYNIQKGKDMPNCDGA +NFKRDTTCFFSRAA+E
Sbjct: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLHN 600
           FFSNIFTTLFGSVRSTILSGTFTALQSFEG DEIDLLCEKEVLQTCSQSTEMSQ+AKLHN
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWL 660
           FGETSL IEEIHKN+DLTFSTSS+NYDL TQFDMASDCPDHHFLSSGKGSMLSQASR WL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660

Query: 661 KKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720
           KKVQQEWSIL+NNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSK PKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCS 900
           QHILAACRAYMEGTPVGSDY  G TK ENPNQSSTGFKIMLAKIFPRLIKTFSSKG DCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

Query: 901 QFAEAENVLIL 911
           QF EAEN  IL
Sbjct: 901 QFMEAENGFIL 911

BLAST of CcUC04G077750 vs. ExPASy TrEMBL
Match: A0A5D3CMG4 (Putative ubiquitin-conjugating enzyme E2 24 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007470 PE=4 SV=1)

HSP 1 Score: 1725.7 bits (4468), Expect = 0.0e+00
Identity = 847/911 (92.97%), Postives = 871/911 (95.61%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVC VNDP+GQMGKVVGI+MLVDLEKN GPLIK+VNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDG  CEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLASIL+GCSSSLTAPP MQHAKELTLLSCF HANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 NLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFP DEKN+YFGEKF +THDL NGGYRGTG  EIC+IVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQLR 360
           TALDS CLTPANA NAHEFWPQQFVMEKGNCDDSSMS+ QRWGVVQAVDAKERTVKVQLR
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEIS 420
           T D SEGS+LDGL+VEETMSAYEL+EHPDYSFSLGDIVFRLA+DKF SQ DENNI+ EIS
Sbjct: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420

Query: 421 MNESAQNGENHII-DNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MNESAQN  NHII +NNMN +NFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK
Sbjct: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAIE 540
           YEVS+IAV NGENLEESR +QIEPLKPYNIQKGKDMPNCDGA +NFKRDTTCFFSRAA+E
Sbjct: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLHN 600
           FFSNIFTTLFGSVRSTILSGTFTALQSFEG DEIDLLCEKEVLQTCSQSTEMSQ+AKLHN
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWL 660
           FGETSL IEEIHKN+DLTFSTSS+NYDL TQFDMASDCPDHHFLSSGKGSMLSQASR WL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660

Query: 661 KKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720
           KKVQQEWSIL+NNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSK PKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCS 900
           QHILAACRAYMEGTPVGSDY  G TK ENPNQSSTGFKIMLAKIFPRLIKTFSSKG DCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

Query: 901 QFAEAENVLIL 911
           QF EAEN  IL
Sbjct: 901 QFMEAENGFIL 911

BLAST of CcUC04G077750 vs. ExPASy TrEMBL
Match: A0A0A0KLH0 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517350 PE=4 SV=1)

HSP 1 Score: 1721.1 bits (4456), Expect = 0.0e+00
Identity = 844/910 (92.75%), Postives = 867/910 (95.27%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 46  MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 105

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVC VNDP+GQMGKVVGIDMLVDLEKN GP+IK+VNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 106 AVCFVNDPYGQMGKVVGIDMLVDLEKNSGPIIKNVNSKDLLKIRSISAGDVVVNGPWIGR 165

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDG  CEIIAVDQEKLLPISVD LDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 166 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDVLDDSQYPYYPGQRVRFSNVSKPPGW 225

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLAS+L+GCSSSLTAPP MQHAKELTLLSCF HANW
Sbjct: 226 LSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFSHANW 285

Query: 241 NLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFP DEKN+YFGEKFITTHDL NGGYRGTG Q+ICVIVKKKTKVDVLWQDGSRS
Sbjct: 286 NLGDWCIFPGDEKNNYFGEKFITTHDLMNGGYRGTGFQDICVIVKKKTKVDVLWQDGSRS 345

Query: 301 TALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQLR 360
           TALDS CLTPANA NAHEFWPQQFVMEKGNCDDSSMS+ QRWGVVQAVDAKERTVKVQLR
Sbjct: 346 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 405

Query: 361 TGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEIS 420
           T D SEGSNLDGL+VEETMSAYEL+EHPDYSFSLGDIVFRLALDKF SQ DENNI+ EIS
Sbjct: 406 TDDLSEGSNLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFASQSDENNIISEIS 465

Query: 421 MNESAQNGENHIIDNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDKY 480
           MNESAQN  N II+NNM  +NFLSFIGNVTGFKDGAVEVKWANCS SMVSPQEIF IDKY
Sbjct: 466 MNESAQNEGNRIINNNMIMDNFLSFIGNVTGFKDGAVEVKWANCSISMVSPQEIFSIDKY 525

Query: 481 EVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAIEF 540
           EVS IAV NGENLEESR +QIEPLKPYNIQKGKDMPNCDGA +NFKRDTTCFFSRAA+EF
Sbjct: 526 EVSPIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVEF 585

Query: 541 FSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLHNF 600
           FSNIFTTLFGSVRSTILSGTFTALQSFEG DEI+LLCEKEVLQTCSQSTEMSQ+AKLHNF
Sbjct: 586 FSNIFTTLFGSVRSTILSGTFTALQSFEGGDEINLLCEKEVLQTCSQSTEMSQIAKLHNF 645

Query: 601 GETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWLK 660
           GETSL IEEIHKN+DLTFSTSSENYDL TQFDMASDCPDHHFLSSGKG MLSQASR WLK
Sbjct: 646 GETSLDIEEIHKNEDLTFSTSSENYDLLTQFDMASDCPDHHFLSSGKGPMLSQASRRWLK 705

Query: 661 KVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIP 720
           KVQQEWSIL+NNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQIP
Sbjct: 706 KVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQIP 765

Query: 721 PLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK 780
           PLVHYISGGLRVNPNLYESG+VCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK
Sbjct: 766 PLVHYISGGLRVNPNLYESGRVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEK 825

Query: 781 PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQ 840
           PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSK PKHFE LVEEHFRRRYQ
Sbjct: 826 PYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFEALVEEHFRRRYQ 885

Query: 841 HILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCSQ 900
           HILAACRAYMEGTPVGSDY  G TK ENP+QSSTGFKIMLAKIFPRLIKTFSSKG DCSQ
Sbjct: 886 HILAACRAYMEGTPVGSDYQCGTTKPENPDQSSTGFKIMLAKIFPRLIKTFSSKGFDCSQ 945

Query: 901 FAEAENVLIL 911
           F EAEN LIL
Sbjct: 946 FMEAENGLIL 955

BLAST of CcUC04G077750 vs. ExPASy TrEMBL
Match: A0A6J1IQQ9 (probable ubiquitin-conjugating enzyme E2 24 OS=Cucurbita maxima OX=3661 GN=LOC111478555 PE=4 SV=1)

HSP 1 Score: 1642.9 bits (4253), Expect = 0.0e+00
Identity = 807/905 (89.17%), Postives = 843/905 (93.15%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDL LSDSDWENSS+CDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 1   MDLILSDSDWENSSDCDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
            VCSVNDPFGQMGKVVGIDMLVDLE NHGP+IKDVNSKDL KIR IS GDVVV+G WIGR
Sbjct: 61  VVCSVNDPFGQMGKVVGIDMLVDLETNHGPIIKDVNSKDLRKIRPISVGDVVVHGKWIGR 120

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           V+KVIDSVSVLFDDGT CEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSN SKPP W
Sbjct: 121 VEKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNASKPPRW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHANW 240
           LSGWKD+RCEGTVCSV+AGLVYVDWLASIL+GCSSSLTAPP MQHAKELT+LSCFPHANW
Sbjct: 181 LSGWKDNRCEGTVCSVDAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTVLSCFPHANW 240

Query: 241 NLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSRS 300
           +LGDWCIFPVDEK+DY GEKFIT HDL  GGYR TGLQEI VIVKKKTKVDVLWQDGS S
Sbjct: 241 HLGDWCIFPVDEKSDYLGEKFITAHDLLTGGYRDTGLQEISVIVKKKTKVDVLWQDGSHS 300

Query: 301 TALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQLR 360
             LDSHCLTPANA+NAHEFWPQQFVMEKGNCDDSSMS TQRWGVVQAVDAKERTVKVQLR
Sbjct: 301 RGLDSHCLTPANAMNAHEFWPQQFVMEKGNCDDSSMSETQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEIS 420
           TGD +EGS+LDGL VEETMSAYEL+EHPDYSF+LGDIVFRLA   FGSQ DENNI  EIS
Sbjct: 361 TGDLTEGSDLDGLWVEETMSAYELVEHPDYSFTLGDIVFRLA--SFGSQSDENNITSEIS 420

Query: 421 MN-ESAQNGENHIIDNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MN ESAQNGE+HII NNMN +NFLSFIGNVT FKDG VEVKWANCS+SMVSP EIFPIDK
Sbjct: 421 MNEESAQNGESHIISNNMNMDNFLSFIGNVTSFKDGTVEVKWANCSSSMVSPHEIFPIDK 480

Query: 481 YEVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAIE 540
           YEVS IAV +G+N+EE RQDQIEP KP NIQKGKDMPN D AG+NF+RDTTCF SRAAIE
Sbjct: 481 YEVSAIAVPSGDNVEELRQDQIEPSKPDNIQKGKDMPNFDAAGKNFRRDTTCFLSRAAIE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLHN 600
           FFSN+FTTLFGSVRSTI+SGTFTALQSFEG D ID+L EKEVLQTCSQ T  SQ+AKL +
Sbjct: 541 FFSNVFTTLFGSVRSTIISGTFTALQSFEGGDNIDILPEKEVLQTCSQFTGRSQIAKLKS 600

Query: 601 FGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWL 660
           FGETSL+ EEIH+N+  T STSSENYDLFTQFDMASDCPDHHFLSSG  SMLSQA RGWL
Sbjct: 601 FGETSLNTEEIHENEASTLSTSSENYDLFTQFDMASDCPDHHFLSSGNASMLSQARRGWL 660

Query: 661 KKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720
           KKVQQEWSIL+NNLPETVYVRVFEERMDLIR+VIIGAPGTPYHDGLFFFDIFLPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRSVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCS 900
           QHILAACRAYMEGTPVG+DYH GA KL NPNQSSTGFKIMLAKI+PRLIKTFSSKGIDCS
Sbjct: 841 QHILAACRAYMEGTPVGADYHSGAKKLVNPNQSSTGFKIMLAKIYPRLIKTFSSKGIDCS 900

Query: 901 QFAEA 905
           QF EA
Sbjct: 901 QFFEA 903

BLAST of CcUC04G077750 vs. ExPASy TrEMBL
Match: A0A6J1CJP8 (probable ubiquitin-conjugating enzyme E2 24 OS=Momordica charantia OX=3673 GN=LOC111012245 PE=4 SV=1)

HSP 1 Score: 1637.5 bits (4239), Expect = 0.0e+00
Identity = 802/912 (87.94%), Postives = 844/912 (92.54%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEY-YHGQTSCILSSLEETIGKIDEFLSFEREFLLG 60
           MDLFLSDSDWENSSECDSSDD EEAEY YHGQTSCILSSLEETIGKIDEFLSFEREF+LG
Sbjct: 1   MDLFLSDSDWENSSECDSSDDPEEAEYFYHGQTSCILSSLEETIGKIDEFLSFEREFMLG 60

Query: 61  DAVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIG 120
           D VCSVNDPFGQMGKVVGIDMLVDLEKNHGP IKDVNSKDLLKIRSIS GDVVV+G WIG
Sbjct: 61  DVVCSVNDPFGQMGKVVGIDMLVDLEKNHGPFIKDVNSKDLLKIRSISVGDVVVHGSWIG 120

Query: 121 RVDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPG 180
           RVDKVIDSVSVLFDDGT CEIIAVDQEKLL ISVDALDD QYPYYPGQRVR SNVSK P 
Sbjct: 121 RVDKVIDSVSVLFDDGTKCEIIAVDQEKLLSISVDALDDPQYPYYPGQRVRLSNVSKSPR 180

Query: 181 WLSGWKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPHAN 240
           WLSGWKDDRCEGTVCSVEAGLVYVDWLAS+L+GCSSSL AP  MQHAK+LTLLSCFPHAN
Sbjct: 181 WLSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLPAPQRMQHAKKLTLLSCFPHAN 240

Query: 241 WNLGDWCIFPVDEKNDYFGEKFITTHDLTNGGYRGTGLQEICVIVKKKTKVDVLWQDGSR 300
           W+LGDWCIFPVDEK DY GEKF TTHDL NGGY GTG QEI +IVKKKTKVDVLWQDGSR
Sbjct: 241 WHLGDWCIFPVDEKGDYLGEKFTTTHDLMNGGYCGTGFQEISIIVKKKTKVDVLWQDGSR 300

Query: 301 STALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQL 360
           S+ LDSHCL PANA+NAHEFWPQQ VMEKG C+D S+S+ +RWGVV+AVDAKERTVKVQ 
Sbjct: 301 SSELDSHCLAPANAMNAHEFWPQQLVMEKGTCNDPSISIAERWGVVRAVDAKERTVKVQW 360

Query: 361 RTGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDKFGSQPDENNIMYEI 420
           RT D +EG++LD L+VEETMSAYEL+EHPDYSFSLGDIVFRLALDKFG Q DE+N  YEI
Sbjct: 361 RTSDLTEGNDLDILLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFGGQSDEHNTNYEI 420

Query: 421 SMN-ESAQNGENHIIDNNMNAENFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPID 480
           SM+ ESAQNGEN +IDN MN +NFLSFIGNV GFKDG +EV+WANCSTS V PQEIFPID
Sbjct: 421 SMSEESAQNGENRVIDNTMNMDNFLSFIGNVIGFKDGVMEVRWANCSTSKVLPQEIFPID 480

Query: 481 KYEVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGQNFKRDTTCFFSRAAI 540
           KYEVST+AV NGEN+EE RQDQIE LKPYNIQKGKDMPNCDGAG+NFKRDTTCFFSRAAI
Sbjct: 481 KYEVSTLAVPNGENVEELRQDQIEHLKPYNIQKGKDMPNCDGAGKNFKRDTTCFFSRAAI 540

Query: 541 EFFSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKEVLQTCSQSTEMSQVAKLH 600
           EFFS+IFTTLFGSVRSTILSGTFTALQSFEG DEI LL EKE LQTC QSTE+SQ+AKL 
Sbjct: 541 EFFSSIFTTLFGSVRSTILSGTFTALQSFEGGDEIGLLNEKEELQTCCQSTELSQIAKLQ 600

Query: 601 NFGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGW 660
           +FGETS  IEE HKN+D T STSSENYDLFTQFDMASDCPDHHFLSSG GS+LSQA RGW
Sbjct: 601 DFGETSFKIEENHKNEDFTLSTSSENYDLFTQFDMASDCPDHHFLSSGNGSLLSQARRGW 660

Query: 661 LKKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQ 720
           LKKVQQEWSIL+NNLPET+YVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQ
Sbjct: 661 LKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQ 720

Query: 721 IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLN 780
           IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNP SSTILQVLLSLQALVLN
Sbjct: 721 IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPVSSTILQVLLSLQALVLN 780

Query: 781 EKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRR 840
           EKPYYNEAGYDAQLGRAEGE+NS SYNENAFLVTCKSMMYLLSKPPKHFE LVEEHFRRR
Sbjct: 781 EKPYYNEAGYDAQLGRAEGEKNSDSYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRRR 840

Query: 841 YQHILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDC 900
           YQHILAAC AYMEGTPVGSDYH GATKLE PNQSSTGFKIMLAKIFP+L++TFSSKGIDC
Sbjct: 841 YQHILAACIAYMEGTPVGSDYHCGATKLEYPNQSSTGFKIMLAKIFPKLVETFSSKGIDC 900

Query: 901 SQFAEAENVLIL 911
           SQF  AENVLI+
Sbjct: 901 SQFIRAENVLIV 912

BLAST of CcUC04G077750 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 860.5 bits (2222), Expect = 1.2e-249
Identity = 455/920 (49.46%), Postives = 609/920 (66.20%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           M++ L+DSDW++SS+  SS+  E    Y G+   I S+LEETIGKIDEFLSFER F+ GD
Sbjct: 1   MEMSLTDSDWDSSSDSGSSEHEEVEFSYGGRAQNIFSNLEETIGKIDEFLSFERGFMYGD 60

Query: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDLLKIRSISAGDVVVNGPWIGR 120
            V S  +P GQ G+V+ IDM V+LE  HG ++K+V++K L K+RSIS  D V+NGPW+GR
Sbjct: 61  IVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRLQKLRSISLSDYVINGPWVGR 120

Query: 121 VDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDALDDSQYPYYPGQR--VRFSNVSKPP 180
           VDK+++ VSV  DDGTN E++   Q+KL+ I  + L+DSQY YYPGQR  V+ ++  +  
Sbjct: 121 VDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQYSYYPGQRVQVKLAHAPRST 180

Query: 181 GWLSG-WKDDRCEGTVCSVEAGLVYVDWLASILIGCSSSLTAPPHMQHAKELTLLSCFPH 240
            WL G W+  +  GTVC+VEAGLVYVDW+ASI++    +LTAP  +Q+ + LTLL C  H
Sbjct: 181 TWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNLTAPQALQNPESLTLLPCVSH 240

Query: 241 ANWNLGDWCIFPVDEKNDY------------FGEKFITTHDLTNGGYRGTGLQEICVIVK 300
           A+W LGDWCI P     D               E   T     N   + +GL E+ VI K
Sbjct: 241 ASWQLGDWCILPGSSHCDIAERQTPNVAAYNLNECHKTFQKGFNRNMQNSGLDELFVITK 300

Query: 301 KKTKVDVLWQDGSRSTALDSHCLTPANAVNAHEFWPQQFVMEKGNCDDSSMSLTQRWGVV 360
            K KV V+WQDGS S  +DS  L P  AVNAH+FWP+QFV+EK  C+      +++WGVV
Sbjct: 301 TKMKVAVMWQDGSCSLGVDSQQLLPVGAVNAHDFWPEQFVVEKETCN------SKKWGVV 360

Query: 361 QAVDAKERTVKVQLRTGDSSEGSNLDGLVVEETMSAYELIEHPDYSFSLGDIVFRLALDK 420
           +AV+AKE+TVKVQ       E +     V+EE +SAYEL+EHPD+ F   D+V +L L +
Sbjct: 361 KAVNAKEQTVKVQWTIQVEKEATGCVDEVMEEIVSAYELLEHPDFGFCFSDVVVKL-LPE 420

Query: 421 FGSQPDENNIMYEISMNESAQNGENHIIDNNMNAENFLSFIGNVTGFKDGAVEVKWANCS 480
               P+ + I+        A   ++ + +++ +   FLS IG VTGFK+G+V+VKWAN S
Sbjct: 421 GKFDPNADTIV--------ATEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWANGS 480

Query: 481 TSMVSPQEIFPIDKYEVS-TIAVSNGENLEESRQ--DQIEPLKPYNIQKG--KDMPNCDG 540
           TS V+P EI+ +++ E S +  VS+  ++++  Q   Q +     + + G  K     + 
Sbjct: 481 TSKVAPCEIWKMERSEYSNSSTVSSEGSVQDLSQKISQSDEASSNHQETGLVKLYSVGES 540

Query: 541 AGQNFKRDTTCFFSRAAIEFFSNIFTTLFGSVRSTILSGTFTALQSFEGEDEIDLLCEKE 600
             +N    ++ F  +AAI F +N+ ++LFG   ST +  + +     E + + ++L ++ 
Sbjct: 541 CNENIPECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQSDSEVLVQET 600

Query: 601 VLQTCSQSTEMSQVAKLHNFGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPDH 660
                +  T   +V          +  + I+K  D T   +S N   F QFDM +DC DH
Sbjct: 601 AESYDNSETNSGEVDMTTTMVNIPIEGKGINKTLDSTLLENSRNQVRFRQFDMVNDCSDH 660

Query: 661 HFLSSGKGSMLSQASRGWLKKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPGTP 720
           HFLSS KG   SQ ++ W+KKVQQEWS L  NLP T+YVRV EERMDL+RA ++GAPGTP
Sbjct: 661 HFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPGTP 720

Query: 721 YHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNP 780
           YHDGLFFFDI LPP+YP  PP+VHY SGG+R+NPNLYESG+VCLSLLNTW+GSG+EVWN 
Sbjct: 721 YHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLLNTWSGSGTEVWNA 780

Query: 781 GSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLL 840
           GSS+ILQ+LLS QALVLNEKPY+NEAGYD QLGRAEGE+NS SYNENAFL+TCKSM+ +L
Sbjct: 781 GSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENAFLITCKSMISML 840

Query: 841 SKPPKHFEKLVEEHFRRRYQHILAACRAYMEGTPVGSDYHFGATKLENPNQSSTGFKIML 900
            KPPKHFE LV++HF  R QH+LAAC+AYMEG PVGS     A    N   +STGFKIML
Sbjct: 841 RKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS----SANLQGNSTTNSTGFKIML 900

BLAST of CcUC04G077750 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 608.2 bits (1567), Expect = 1.1e-173
Identity = 372/985 (37.77%), Postives = 533/985 (54.11%), Query Frame = 0

Query: 41   ETIGKIDEFLSFEREFLLGDAVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKDL 100
            E +  I++    +R FL GD V S ++P GQ+G VV +++ VDL    G + KD+++K+L
Sbjct: 130  EPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAPDGSIHKDISTKNL 189

Query: 101  LKIRSISAGDVVVNGPWIGRVDKVIDSVSVLFDDGTNCEIIAVDQEKLLPISVDAL-DDS 160
             ++R  + GD VV+GPW+GR+D V+D+V+VLFDDG+ C+++ V+  +L PI  + L +D+
Sbjct: 190  KRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLRLKPIPKNNLEEDA 249

Query: 161  QYPYYPGQRVR--FSNVSKPPGWLSG-WKDDRCEGTVCSVEAGLVYVDWLASILIGCSSS 220
             +PYYPGQRV+   S+V K   WLSG WK +R EGTV  V AG ++V W+AS   G  SS
Sbjct: 250  NFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFVYWIASAGFGPDSS 309

Query: 221  LTAPPHMQHAKELTLLSCFPHANWNLGDWCIFP----------------------VDEKN 280
            + +PP  Q+   LTLLSCF HANW +GDWC+ P                        ++ 
Sbjct: 310  V-SPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVSKLRLYDSQADRQ 369

Query: 281  DYFGEKFITTHDLTNGGYRGTGL------------------------------------- 340
               G       D  +G     G+                                     
Sbjct: 370  QKIGRDLEDVQDEVSGKVEPAGITAEALPKVTSDDPPQRNPSVSKEPVHEPWPLHRKKIR 429

Query: 341  -----------------QEICVIVKKKTKVDVLWQDGSRSTALDSHCLTPANAVNAHEFW 400
                             ++  ++V  +T+VDV WQDG+     ++  L P      HEF 
Sbjct: 430  KLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQDGTIECRREAITLIPIETPGDHEFV 489

Query: 401  PQQFVMEKGNCDDSSMSLTQRWGVVQAVDAKERTVKVQ----LRTGDSSEGSNLDGLVVE 460
             +Q+V+EK + D  + +  +R GVV+ V+AK+RT  V+    LR  +            E
Sbjct: 490  SEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTASVRWLNPLRRAEEPR-----EFEKE 549

Query: 461  ETMSAYELIEHPDYSFSLGDIVFRL---ALDKFGSQPDENNIMYEISMNESAQNGENH-- 520
            E +S YEL  HPDY +  GD+V RL   A+    S P  N+       +   Q+ E+H  
Sbjct: 550  EIVSVYELEGHPDYDYCYGDVVVRLSPIAVALPASSPG-NSFEEATQQDNGYQDSESHQE 609

Query: 521  ---IIDNNMNAENF----LSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK----- 580
               ++D   N  +     LS++GN+TG KDG +EV WA+ + S V P  ++ + +     
Sbjct: 610  AKILVDKEENEPSTDLSKLSWVGNITGLKDGDIEVTWADGTISTVGPHAVYVVGRDDDDE 669

Query: 581  ---YEVSTIAVSNGENLEESRQDQIEPLKPYNIQKGKDMPNCDG---AGQNFKRDTTCFF 640
                E  T   ++ E L +  +   E  +    +      N D    A  +  R+     
Sbjct: 670  SVAGESETSDAASWETLNDDDRGAPEIPEEDLGRSSSIEGNSDADIYAENDSGRNGALAL 729

Query: 641  SRAAIEFFSNIFTTLFGSVRSTI--------LSGTFTALQSFEGEDEIDLL-----CEKE 700
              AAIEF + + + +F   R ++        +   +   +S    DE D L      +  
Sbjct: 730  PLAAIEFVTRLASGIFSRARKSVDSSSSDYTVENVYKQAESTNPSDETDSLDDPSPSKVN 789

Query: 701  VLQTC-SQSTEMSQVAKLHNFGETSLSIEEIHKNDDLTFSTSSENYDLFTQFDMASDCPD 760
            V   C S+ T+ +  AK    GETS  +E+    +D    +  ++   F +FD++ D  D
Sbjct: 790  VTDNCESKGTQAN--AKNILSGETSTFLED----EDKPVPSEGDSCS-FRRFDISQDPLD 849

Query: 761  HHFLS-SGKGSMLSQASRGWLKKVQQEWSILRNNLPETVYVRVFEERMDLIRAVIIGAPG 820
            HHFL   G+        R W KKV Q+W IL+NNLP+ ++VR +E+RMDL+RAVI+GA G
Sbjct: 850  HHFLGVDGQ----KTKERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFG 909

Query: 821  TPYHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVW 880
            TPY DGLFFFD  LP +YP +PP  +Y SGG R+NPNLYE GKVCLSLLNTWTG G+EVW
Sbjct: 910  TPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVW 969

Query: 881  NPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMY 902
            +P SS+ILQVL+SLQ LVLN KPY+NEAGYD Q+G AEGE+NS  YNEN FL+ CK+MMY
Sbjct: 970  DPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMY 1029

BLAST of CcUC04G077750 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 297.4 bits (760), Expect = 4.0e-80
Identity = 145/270 (53.70%), Postives = 178/270 (65.93%), Query Frame = 0

Query: 628 FTQFDMASDCPDHHFLSSGKGSMLSQASRGWLKKVQQEWSILRNNLPETVYVRVFEERMD 687
           F +FD   D  DHH+ S GK S   Q S+ W+KKVQ +W IL N+LPE + VR  E RMD
Sbjct: 244 FKRFDTVEDFSDHHYASKGKSS--KQHSKNWVKKVQADWKILENDLPEAISVRACESRMD 303

Query: 688 LIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLL 747
           L+RAVIIGA GTPYHDGLFFFDI  P  YP +PP VHY SGGLR+NPNLY  GKVCLSLL
Sbjct: 304 LLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLL 363

Query: 748 NTWTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNEN 807
            TW GS  E W P  ST+LQ+L+S+QAL+LNEKPY+NE GY    G A GE  S  Y+EN
Sbjct: 364 GTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSEN 423

Query: 808 AFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQHILAACRAYMEGTPVGSDYHFGATKLE 867
            FL++ K+M+Y + +PP+HFE+ V+ H+  R   I+ AC AY  G P+GS    G   LE
Sbjct: 424 VFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLE 483

Query: 868 NPNQS-STGFKIMLAKIFPRLIKTFSSKGI 897
              QS S  FK  +A     ++  F   G+
Sbjct: 484 EARQSGSKKFKTDVASFMQTVVDEFVKLGV 511

BLAST of CcUC04G077750 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 279.3 bits (713), Expect = 1.1e-74
Identity = 127/232 (54.74%), Postives = 162/232 (69.83%), Query Frame = 0

Query: 625 YDLFTQFDMASDCPDHHFLSSGKGSMLSQASRGWLKKVQQEWSILRNNLPETVYVRVFEE 684
           Y  F +FD+  D  DHH+ S  KG+   Q S+ W K++Q EW IL  +LPE ++VR +E 
Sbjct: 302 YLFFKRFDIVEDFSDHHYAS--KGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYES 361

Query: 685 RMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCL 744
           RMDL+RAVIIGA GTPYHDGLFFFDIF P  YP  PP+VHY SGGLR+NPNLY  GKVCL
Sbjct: 362 RMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCL 421

Query: 745 SLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASY 804
           SLL TW+G+  E W P +ST+LQVL+S+Q L+LN+KPY+NE GY+   G A GE  S +Y
Sbjct: 422 SLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAY 481

Query: 805 NENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQHILAACRAYMEGTPVG 857
           +EN F+++ K+M+Y + +PPK+FE     HF      +L AC AY  G   G
Sbjct: 482 SENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPG 531

BLAST of CcUC04G077750 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 270.0 bits (689), Expect = 6.9e-72
Identity = 134/275 (48.73%), Postives = 172/275 (62.55%), Query Frame = 0

Query: 628 FTQFDMASDCPDHHFLSSGKGSMLS-------QASRGWLKKVQQEWSILRNNLPETVYVR 687
           F +FD   D  DHH+ + G  + LS          + W+K +Q+EW IL  NLPET++VR
Sbjct: 21  FKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVR 80

Query: 688 VFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQIPPLVHYISGGLRVNPNLYESG 747
             E R+DL+RAVIIGA GTPYHDGLFFFDI  P  YP +PP VHY SGGLR+NPNLY+ G
Sbjct: 81  ACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRINPNLYKCG 140

Query: 748 KVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERN 807
           KVCLSL++TWTG   E W P  ST+LQ+L+S+QAL+LNEKPYYNE GY+  +G   GE  
Sbjct: 141 KVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESY 200

Query: 808 SASYNENAFLVTCKSMMYLLSKPPKHFEKLVEEHFRRRYQHILAACRAYMEGTPVGSDYH 867
           S  Y+EN F+ + K+M         HFE+ V  HF  R   I+ AC AY +G PVGS   
Sbjct: 201 SKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYKDGAPVGS-ID 260

Query: 868 FGATKLENPNQSSTGFKIMLAKIFPRLIKTFSSKG 896
            G  K +   + S  F+I +      ++  F + G
Sbjct: 261 KGGVKKQTRQRGSLKFRINVTSFMKTVVDEFVNLG 285