CcUC03G045010 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC03G045010
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionHigh-affinity nitrate transporter 2.2
LocationCicolChr03: 2982970 .. 2985000 (-)
RNA-Seq ExpressionCcUC03G045010
SyntenyCcUC03G045010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAGACAAAGAAAAAAGGAAAATGGGAGATGTTGAAGGTTCCCCAGGAAGCTCTATGCATGGGGTGACCGGAAGAGAGCAAAGTTTTGCATTTTCCGTAGCTTCCCCAATTGTCCCAACGGACACAACAGCGAAATTTGCATTACCCGTTGACTCTGAGCACAAAGCGAAGGTTTTCAAGGTGTGGTCTTTAGCCAACCCCCACATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTTACATGCTTCGTCTCCACATTCGCAGCCGCCCCTCTGGTCCCCATCATTCGAGACAATCTCAACCTTACCAAAGTTGACATCGGTAACGCCGGAGTCACCTCCGTGTCTGGCAGTATCTTCTCTCGTCTTGTAATGGGGGCTGTGTGTGACCTTCTGGGGCCACGCTATGGGTGTGCTTTTCTCATTATGCTCTCCGCCCCCACCGTGTTTTGTATGTCCTTTGTGTCCAACGCTGCTGGGTATATAGCTGTGAGATTCATGATTGGCTTCTCGCTTGCCACCTTCGTCTCCTGCCAGTATTGGATGAGCACCATGTTCAACAGTCAGATCATTGGGCTTGTGAATGGGACTGCTGCTGGGTGGGGGAATATGGGCGGAGGAGCCACTCAGCTCATCATGCCTTTGTTGTACGATGTCATTCAGAGAGCTGGCGCTACTCCTTTCACTGCTTGGAGGATTGCCTTCTTTATCCCTGGTTCCCTTCATATTCTCATGGGCATTTTGGTCTTAACTCTTGGCCAGGACTTGCCTGATGGTAACCTTACCAGTCTTCAGAAGAAGGGCAATGTTGCCAAAGATAAATTCTCCAATGTAATTACTTCCTTCCTTCTCCATCTATATACATATATATCTCTTCTCAATTCATTCTTTAATTTTACTTTAATTATAATCATCTCTCCAATATAATATAATATCTCCTCACAGATCCTGTGGTATGCTGTCACCAATTACCGAACTTGGATCTTCGTCCTCCTCTATGGCTTCTCCATGGGGGTCGAACTTTCCACCGATAACGTCATTGCCGAATACTTCTTCGACAGGTAAAATAATCAAATCTGATTTTTTAACCTAATATCAAACCCCGTCAGTCAATCCTAATCCTATGCATATGTGTTATGAAATCAACAATCATAGAAAGTGATCACAAGACGAAAACAATATTATGAAAAACCCACTTATCCGTGTGTTGCCCAAGTCCACGTACAAATTGAGACACCATTTACATTAACACAGAAATATCTAGGTTTTAGATTCTCCACCAAGGGCGCGTTTTTTTACGTGTCCTATAACATATTCAAAGCATTCCGACCTTACGTTTTGAATTTGTGTTTGTCATATTTAACAGGTTTGATCTAAAGCTACACACGGCAGGAATTATCGCAGCAACGTTCGGGATGGCGAATCTAGTGGCTCGACCGTTCGGGGGGTATGCATCAGACGTGGCAGCGCGGTACTTCGGGATGCGAGGAAGGCTGTGGACTTTGTGGATCCTACAAACATGTGGGGGAGTGTTTTGCATGTGGCTTGGGAAAGCAAACAAACTGCCAACAGCGATAGTTGCAATGATATTGTTCTCAATAGGAGCACAGGCTGCATGTGGAGCAACATTTGGGATCATTCCCTTCATTTCTCGACGGTCTTTAGGAATTATATCTGGACTGACAGGAGCAGGAGGCAACTTTGGAAGTGGTTTGACACAATTAGTGTTCTTCTCAACCACCAAATACTCAACAGACACAGGCTTGTTCTTGATGGGAATAATGATTGTATGTTGCACTTTACCAGTCAGTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTTTACCTCCCACGAAACATGTTGAAAAATCCACTGAGGAATTTTATTATGGCTCTGAATGGACTGAGGATGAAAAGAAGAAGGGTTTGCATCAACAAAGCATTAAGTTTGCTGAGAATAGCAGATCTGAACGTGGTAGGCGTGTGGCTTCAGCTCCAACTCCACCAAATACAACTCCTTCCCATGTT

mRNA sequence

TAAAGACAAAGAAAAAAGGAAAATGGGAGATGTTGAAGGTTCCCCAGGAAGCTCTATGCATGGGGTGACCGGAAGAGAGCAAAGTTTTGCATTTTCCGTAGCTTCCCCAATTGTCCCAACGGACACAACAGCGAAATTTGCATTACCCGTTGACTCTGAGCACAAAGCGAAGGTTTTCAAGGTGTGGTCTTTAGCCAACCCCCACATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTTACATGCTTCGTCTCCACATTCGCAGCCGCCCCTCTGGTCCCCATCATTCGAGACAATCTCAACCTTACCAAAGTTGACATCGGTAACGCCGGAGTCACCTCCGTGTCTGGCAGTATCTTCTCTCGTCTTGTAATGGGGGCTGTGTGTGACCTTCTGGGGCCACGCTATGGGTGTGCTTTTCTCATTATGCTCTCCGCCCCCACCGTGTTTTGTATGTCCTTTGTGTCCAACGCTGCTGGGTATATAGCTGTGAGATTCATGATTGGCTTCTCGCTTGCCACCTTCGTCTCCTGCCAGTATTGGATGAGCACCATGTTCAACAGTCAGATCATTGGGCTTGTGAATGGGACTGCTGCTGGGTGGGGGAATATGGGCGGAGGAGCCACTCAGCTCATCATGCCTTTGTTGTACGATGTCATTCAGAGAGCTGGCGCTACTCCTTTCACTGCTTGGAGGATTGCCTTCTTTATCCCTGGTTCCCTTCATATTCTCATGGGCATTTTGGTCTTAACTCTTGGCCAGGACTTGCCTGATGGTAACCTTACCAGTCTTCAGAAGAAGGGCAATGTTGCCAAAGATAAATTCTCCAATATCCTGTGGTATGCTGTCACCAATTACCGAACTTGGATCTTCGTCCTCCTCTATGGCTTCTCCATGGGGGTCGAACTTTCCACCGATAACGTCATTGCCGAATACTTCTTCGACAGACGAAAACAATATTATGAAAAACCCACTTATCCGTGTGTTGCCCAAGTCCACGTACAAATTGAGACACCATTTACATTAACACAGAAATATCTAGGTTTTAGATTCTCCACCAAGGGCGCGTTTTTTTACGTGTCCTATAACATATTCAAAGCATTCCGACCTTACGTTTTGAATTTGTTTGATCTAAAGCTACACACGGCAGGAATTATCGCAGCAACGTTCGGGATGGCGAATCTAGTGGCTCGACCGTTCGGGGGGTATGCATCAGACGTGGCAGCGCGGTACTTCGGGATGCGAGGAAGGCTGTGGACTTTGTGGATCCTACAAACATGTGGGGGAGTGTTTTGCATGTGGCTTGGGAAAGCAAACAAACTGCCAACAGCGATAGTTGCAATGATATTGTTCTCAATAGGAGCACAGGCTGCATGTGGAGCAACATTTGGGATCATTCCCTTCATTTCTCGACGGTCTTTAGGAATTATATCTGGACTGACAGGAGCAGGAGGCAACTTTGGAAGTGGTTTGACACAATTAGTGTTCTTCTCAACCACCAAATACTCAACAGACACAGGCTTGTTCTTGATGGGAATAATGATTGTATGTTGCACTTTACCAGTCAGTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTTTACCTCCCACGAAACATGTTGAAAAATCCACTGAGGAATTTTATTATGGCTCTGAATGGACTGAGGATGAAAAGAAGAAGGGTTTGCATCAACAAAGCATTAAGTTTGCTGAGAATAGCAGATCTGAACGTGGTAGGCGTGTGGCTTCAGCTCCAACTCCACCAAATACAACTCCTTCCCATGTT

Coding sequence (CDS)

ATGGGAGATGTTGAAGGTTCCCCAGGAAGCTCTATGCATGGGGTGACCGGAAGAGAGCAAAGTTTTGCATTTTCCGTAGCTTCCCCAATTGTCCCAACGGACACAACAGCGAAATTTGCATTACCCGTTGACTCTGAGCACAAAGCGAAGGTTTTCAAGGTGTGGTCTTTAGCCAACCCCCACATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTTACATGCTTCGTCTCCACATTCGCAGCCGCCCCTCTGGTCCCCATCATTCGAGACAATCTCAACCTTACCAAAGTTGACATCGGTAACGCCGGAGTCACCTCCGTGTCTGGCAGTATCTTCTCTCGTCTTGTAATGGGGGCTGTGTGTGACCTTCTGGGGCCACGCTATGGGTGTGCTTTTCTCATTATGCTCTCCGCCCCCACCGTGTTTTGTATGTCCTTTGTGTCCAACGCTGCTGGGTATATAGCTGTGAGATTCATGATTGGCTTCTCGCTTGCCACCTTCGTCTCCTGCCAGTATTGGATGAGCACCATGTTCAACAGTCAGATCATTGGGCTTGTGAATGGGACTGCTGCTGGGTGGGGGAATATGGGCGGAGGAGCCACTCAGCTCATCATGCCTTTGTTGTACGATGTCATTCAGAGAGCTGGCGCTACTCCTTTCACTGCTTGGAGGATTGCCTTCTTTATCCCTGGTTCCCTTCATATTCTCATGGGCATTTTGGTCTTAACTCTTGGCCAGGACTTGCCTGATGGTAACCTTACCAGTCTTCAGAAGAAGGGCAATGTTGCCAAAGATAAATTCTCCAATATCCTGTGGTATGCTGTCACCAATTACCGAACTTGGATCTTCGTCCTCCTCTATGGCTTCTCCATGGGGGTCGAACTTTCCACCGATAACGTCATTGCCGAATACTTCTTCGACAGACGAAAACAATATTATGAAAAACCCACTTATCCGTGTGTTGCCCAAGTCCACGTACAAATTGAGACACCATTTACATTAACACAGAAATATCTAGGTTTTAGATTCTCCACCAAGGGCGCGTTTTTTTACGTGTCCTATAACATATTCAAAGCATTCCGACCTTACGTTTTGAATTTGTTTGATCTAAAGCTACACACGGCAGGAATTATCGCAGCAACGTTCGGGATGGCGAATCTAGTGGCTCGACCGTTCGGGGGGTATGCATCAGACGTGGCAGCGCGGTACTTCGGGATGCGAGGAAGGCTGTGGACTTTGTGGATCCTACAAACATGTGGGGGAGTGTTTTGCATGTGGCTTGGGAAAGCAAACAAACTGCCAACAGCGATAGTTGCAATGATATTGTTCTCAATAGGAGCACAGGCTGCATGTGGAGCAACATTTGGGATCATTCCCTTCATTTCTCGACGGTCTTTAGGAATTATATCTGGACTGACAGGAGCAGGAGGCAACTTTGGAAGTGGTTTGACACAATTAGTGTTCTTCTCAACCACCAAATACTCAACAGACACAGGCTTGTTCTTGATGGGAATAATGATTGTATGTTGCACTTTACCAGTCAGTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTTTACCTCCCACGAAACATGTTGAAAAATCCACTGAGGAATTTTATTATGGCTCTGAATGGACTGAGGATGAAAAGAAGAAGGGTTTGCATCAACAAAGCATTAAGTTTGCTGAGAATAGCAGATCTGAACGTGGTAGGCGTGTGGCTTCAGCTCCAACTCCACCAAATACAACTCCTTCCCATGTT

Protein sequence

MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGILVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDNVIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKAFRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV
Homology
BLAST of CcUC03G045010 vs. NCBI nr
Match: XP_038895485.1 (high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 high affinity nitrate transporter 2.4-like [Benincasa hispida])

HSP 1 Score: 1010.7 bits (2612), Expect = 5.0e-291
Identity = 517/589 (87.78%), Postives = 525/589 (89.13%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKF+LPVDSEHKAKVFK+WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFSLPVDSEHKAKVFKLWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNV KDKFSN+LWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLGSLQKKGNVTKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYF+DR                                                   
Sbjct: 301 VIAEYFYDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 361 --------FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFSTTKYSTDTGLF MGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 481 GSGLTQLVFFSTTKYSTDTGLFFMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV
Sbjct: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530

BLAST of CcUC03G045010 vs. NCBI nr
Match: XP_031736393.1 (high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP53714.1 high affinity nitrate transporter 2.1 [Cucumis sativus] >KAE8652752.1 hypothetical protein Csa_014056 [Cucumis sativus])

HSP 1 Score: 1006.5 bits (2601), Expect = 9.4e-290
Identity = 514/589 (87.27%), Postives = 524/589 (88.96%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYF+DR                                                   
Sbjct: 301 VIAEYFYDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 361 --------FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CcUC03G045010 vs. NCBI nr
Match: NP_001274401.1 (high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-affinity nitrate transporter [Cucumis sativus])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.6e-289
Identity = 514/589 (87.27%), Postives = 523/589 (88.79%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYF+DR                                                   
Sbjct: 301 VIAEYFYDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 361 --------FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPS V
Sbjct: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of CcUC03G045010 vs. NCBI nr
Match: AGO64298.1 (high-affinity nitrate transporter NRT2.2 [Cucumis sativus])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.6e-289
Identity = 512/589 (86.93%), Postives = 524/589 (88.96%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYF+DR                                                   
Sbjct: 301 VIAEYFYDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 361 --------FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CcUC03G045010 vs. NCBI nr
Match: NP_001269063.2 (high affinity nitrate transporter 2.4-like [Cucumis sativus])

HSP 1 Score: 1000.3 bits (2585), Expect = 6.7e-288
Identity = 511/589 (86.76%), Postives = 522/589 (88.62%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYF+DR                                                   
Sbjct: 301 VIAEYFYDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLW LWILQT 
Sbjct: 361 --------FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWALWILQTF 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPS V
Sbjct: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of CcUC03G045010 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 4.4e-242
Identity = 425/589 (72.16%), Postives = 472/589 (80.14%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGD  G PGSSMHGVTGREQSFAFSV SPIV TD TAKF LPVD+EHKA VFK++S A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG LHI+MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVL LGQDLPDGN  +L+K G VAKDKF  ILWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYFFDR                                                   
Sbjct: 301 VIAEYFFDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   F LKLHTAG+IAA FGMAN  ARP GGYASD AA+YFGMRGRLWTLWI+QT 
Sbjct: 361 --------FHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTA 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GG+FC+WLG+AN L TA+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 421 GGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQL+FFST+ ++T+ GL  MG+MIV CTLPV+LVHFPQWGSMFLPP+    K TE 
Sbjct: 481 GSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEA 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
            YYGSEW E EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 541 HYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of CcUC03G045010 vs. ExPASy Swiss-Prot
Match: Q9FJH8 (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 1.3e-241
Identity = 422/589 (71.65%), Postives = 474/589 (80.48%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           M D  G PGSSMHGVTGREQS+AFSV SP VP+D++AKF+LPVD+EHKAKVFK+ S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFV  A GYI VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
            QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF+PG +HI+MGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGN ++L+KKG V KDKFS +LWYA+TNYRTW+FVLLYG+SMGVEL+TDN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYFFDR                                                   
Sbjct: 301 VIAEYFFDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   F LKLHTAGIIAA+FGMAN  ARP GG+ASD+AAR FGMRGRLWTLWI+QT 
Sbjct: 361 --------FHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTL 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GG FC+WLG+A  LPTA+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFST+ +ST+ GL  MG+MI+ CTLPV+LVHFPQWGSMFLP T+   KSTEE
Sbjct: 481 GSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEE 527

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           +YY  EWTE EK+KG+H+ S+KFA NSRSERGRRVASAP+PP   P HV
Sbjct: 541 YYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of CcUC03G045010 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 5.6e-229
Identity = 404/588 (68.71%), Postives = 458/588 (77.89%), Query Frame = 0

Query: 6   GSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPHMRTF 65
           G+PGSS+HGVTGRE +FAFS           +KF LPVDSEHKAK  ++ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 125
           HLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 185
           GPRYGCAFLIML+APTVFCMS + +AAGYIAVRF+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGILVLTL 245
           LVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F+PG+LH++MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDNVIAEY 305
           GQDLPDGNL SLQKKG+V +D FS +LWYAVTNYRTWIFVLLYG+SMGVEL+TDNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 FFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKAFRPYV 365
           F+DR                                                        
Sbjct: 307 FYDR-------------------------------------------------------- 366

Query: 366 LNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVFC 425
              FDL L  AGIIAA+FGMAN+VARP GG  SD+ ARYFGMR RLW +WILQT GG FC
Sbjct: 367 ---FDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAFC 426

Query: 426 MWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLT 485
           + LG+A+ LPT++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GLT
Sbjct: 427 LLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGLT 486

Query: 486 QLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEEFYYGS 545
           QL+FF++++YST TGL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYGS
Sbjct: 487 QLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYGS 532

Query: 546 EWTEDEKKKGLHQQSIKFAENSRSERGRR-----VASAPTPPNTTPSH 589
           EW+E EK KGLH  S+KFAENSRSERGRR      A+A TPPN +P H
Sbjct: 547 EWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of CcUC03G045010 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 5.6e-229
Identity = 404/588 (68.71%), Postives = 458/588 (77.89%), Query Frame = 0

Query: 6   GSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPHMRTF 65
           G+PGSS+HGVTGRE +FAFS           +KF LPVDSEHKAK  ++ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 125
           HLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 185
           GPRYGCAFLIML+APTVFCMS + +AAGYIAVRF+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGILVLTL 245
           LVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F+PG+LH++MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDNVIAEY 305
           GQDLPDGNL SLQKKG+V +D FS +LWYAVTNYRTWIFVLLYG+SMGVEL+TDNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 FFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKAFRPYV 365
           F+DR                                                        
Sbjct: 307 FYDR-------------------------------------------------------- 366

Query: 366 LNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVFC 425
              FDL L  AGIIAA+FGMAN+VARP GG  SD+ ARYFGMR RLW +WILQT GG FC
Sbjct: 367 ---FDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAFC 426

Query: 426 MWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLT 485
           + LG+A+ LPT++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GLT
Sbjct: 427 LLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGLT 486

Query: 486 QLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEEFYYGS 545
           QL+FF++++YST TGL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYGS
Sbjct: 487 QLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYGS 532

Query: 546 EWTEDEKKKGLHQQSIKFAENSRSERGRR-----VASAPTPPNTTPSH 589
           EW+E EK KGLH  S+KFAENSRSERGRR      A+A TPPN +P H
Sbjct: 547 EWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of CcUC03G045010 vs. ExPASy Swiss-Prot
Match: Q9LMZ9 (High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 5.3e-219
Identity = 390/575 (67.83%), Postives = 452/575 (78.61%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MG  +  PGSSMHGVTGREQS+AFSV     PT+T  K+ LPVD+E KA VFK++S A P
Sbjct: 1   MGSTD-EPGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG LHI+MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLP GN  +++K G VAKDKF  ILWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYFFDR                                                   
Sbjct: 301 VIAEYFFDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   F LKLHTAGIIAA FGMAN  ARP GG+ASD+AA+ FGMRGRLWTLWI+QT 
Sbjct: 361 --------FHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTS 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GG+FC+WLG+AN L TA+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 421 GGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFST++++T+ GL  MG+MIV CTLPV+L+HFPQWGSMF PP+     +TE 
Sbjct: 481 GSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEH 513

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRV 576
           +Y G E++++E++ G+H +S  FA+ +++E G  V
Sbjct: 541 YYVG-EYSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of CcUC03G045010 vs. ExPASy TrEMBL
Match: A0A3Q9CZ42 (High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.5e-290
Identity = 514/589 (87.27%), Postives = 524/589 (88.96%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYF+DR                                                   
Sbjct: 301 VIAEYFYDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 361 --------FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CcUC03G045010 vs. ExPASy TrEMBL
Match: Q6PRP8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.7e-289
Identity = 514/589 (87.27%), Postives = 523/589 (88.79%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYF+DR                                                   
Sbjct: 301 VIAEYFYDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 361 --------FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPS V
Sbjct: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of CcUC03G045010 vs. ExPASy TrEMBL
Match: S4VM42 (High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 2.2e-289
Identity = 512/589 (86.93%), Postives = 524/589 (88.96%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPG LH+LMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNVAKDKFSN+LWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYF+DR                                                   
Sbjct: 301 VIAEYFYDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 361 --------FDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CcUC03G045010 vs. ExPASy TrEMBL
Match: A0A6J1HIH1 (high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC111464708 PE=4 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 8.3e-284
Identity = 503/589 (85.40%), Postives = 520/589 (88.29%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           M  VEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVFK++S ANP
Sbjct: 1   MSGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLYD+I+RAGATPFTAWRIAFFIPG LH++MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGNL+SLQKKG+VAKDKFS +LWYA+TNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYF+DR                                                   
Sbjct: 301 VIAEYFYDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   FDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT 
Sbjct: 361 --------FDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTF 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GGVFCMWLGKA+KLPTAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 481 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           FYYG+EWTEDEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 541 FYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of CcUC03G045010 vs. ExPASy TrEMBL
Match: A0A6J1DHB7 (high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC111020453 PE=4 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 3.1e-283
Identity = 498/588 (84.69%), Postives = 520/588 (88.44%), Query Frame = 0

Query: 2   GDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPH 61
           G+VEGSPGSSMHGVTGREQ+FAFSVASPIVPTDT+AKFALPVDSEHKAKVFK++SLANPH
Sbjct: 6   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 65

Query: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 66  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 125

Query: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS+ AGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 126 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 185

Query: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGIL 241
           QIIGLVNGTAAGWGNMGGGATQL+MPLLY++I++ GATPFTAWRIAFFIPGSLH+LMGIL
Sbjct: 186 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 245

Query: 242 VLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDNV 301
           V+TLGQDLPDGNL+SLQKKG VAKDKFSN+LWYAVTNYRTWIFVLLYG+SMGVELSTDNV
Sbjct: 246 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 305

Query: 302 IAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKAF 361
           +AEYF+DR                                                    
Sbjct: 306 VAEYFYDR---------------------------------------------------- 365

Query: 362 RPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCG 421
                  FDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQTCG
Sbjct: 366 -------FDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCG 425

Query: 422 GVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 481
           GVFCMWLGKA +LP AIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG
Sbjct: 426 GVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 485

Query: 482 SGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEEF 541
           SGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPV+LVHFPQWGSMFLPPTKHVEKSTEEF
Sbjct: 486 SGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF 534

Query: 542 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           YYGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TPSHV
Sbjct: 546 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 534

BLAST of CcUC03G045010 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 838.6 bits (2165), Expect = 3.1e-243
Identity = 425/589 (72.16%), Postives = 472/589 (80.14%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGD  G PGSSMHGVTGREQSFAFSV SPIV TD TAKF LPVD+EHKA VFK++S A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG LHI+MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVL LGQDLPDGN  +L+K G VAKDKF  ILWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYFFDR                                                   
Sbjct: 301 VIAEYFFDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   F LKLHTAG+IAA FGMAN  ARP GGYASD AA+YFGMRGRLWTLWI+QT 
Sbjct: 361 --------FHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTA 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GG+FC+WLG+AN L TA+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 421 GGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQL+FFST+ ++T+ GL  MG+MIV CTLPV+LVHFPQWGSMFLPP+    K TE 
Sbjct: 481 GSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEA 530

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
            YYGSEW E EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 541 HYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of CcUC03G045010 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 837.0 bits (2161), Expect = 9.2e-243
Identity = 422/589 (71.65%), Postives = 474/589 (80.48%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           M D  G PGSSMHGVTGREQS+AFSV SP VP+D++AKF+LPVD+EHKAKVFK+ S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFV  A GYI VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
            QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF+PG +HI+MGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLPDGN ++L+KKG V KDKFS +LWYA+TNYRTW+FVLLYG+SMGVEL+TDN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYFFDR                                                   
Sbjct: 301 VIAEYFFDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   F LKLHTAGIIAA+FGMAN  ARP GG+ASD+AAR FGMRGRLWTLWI+QT 
Sbjct: 361 --------FHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTL 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GG FC+WLG+A  LPTA+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGNF
Sbjct: 421 GGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFST+ +ST+ GL  MG+MI+ CTLPV+LVHFPQWGSMFLP T+   KSTEE
Sbjct: 481 GSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEE 527

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 590
           +YY  EWTE EK+KG+H+ S+KFA NSRSERGRRVASAP+PP   P HV
Sbjct: 541 YYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of CcUC03G045010 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 761.9 bits (1966), Expect = 3.7e-220
Identity = 390/575 (67.83%), Postives = 452/575 (78.61%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MG  +  PGSSMHGVTGREQS+AFSV     PT+T  K+ LPVD+E KA VFK++S A P
Sbjct: 1   MGSTD-EPGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG LHI+MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDN 300
           LVLTLGQDLP GN  +++K G VAKDKF  ILWYAVTNYRTWIFVLLYG+SMGVELSTDN
Sbjct: 241 LVLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKA 360
           VIAEYFFDR                                                   
Sbjct: 301 VIAEYFFDR--------------------------------------------------- 360

Query: 361 FRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 420
                   F LKLHTAGIIAA FGMAN  ARP GG+ASD+AA+ FGMRGRLWTLWI+QT 
Sbjct: 361 --------FHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTS 420

Query: 421 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 480
           GG+FC+WLG+AN L TA+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 421 GGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 480

Query: 481 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEE 540
           GSGLTQLVFFST++++T+ GL  MG+MIV CTLPV+L+HFPQWGSMF PP+     +TE 
Sbjct: 481 GSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEH 513

Query: 541 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRV 576
           +Y G E++++E++ G+H +S  FA+ +++E G  V
Sbjct: 541 YYVG-EYSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of CcUC03G045010 vs. TAIR 10
Match: AT5G60780.1 (nitrate transporter 2.3 )

HSP 1 Score: 758.1 bits (1956), Expect = 5.4e-219
Identity = 386/590 (65.42%), Postives = 450/590 (76.27%), Query Frame = 0

Query: 5   EGSPGSSMHGVTGREQSFAFSV---ASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPH 64
           EGS G+S+HGVT REQ F+FSV   +  +   D TAKFALPVDSEH+AKVF   S A PH
Sbjct: 9   EGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFAKPH 68

Query: 65  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 124
           MR FHL W+SFFTCF+STFAAAPLVPIIRDNL+LTK DIGNAGV SVSG+IFSRL MGAV
Sbjct: 69  MRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMGAV 128

Query: 125 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 184
           CDLLG RYG AF +ML+APTVF MSFV   +GY+ VRFMIGF LATFVSCQYW S MFN 
Sbjct: 129 CDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMFNG 188

Query: 185 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILMGIL 244
           +IIGLVNG A GWG+MGGG TQL+MP+++ VI+ AGATPF AWRIAFF+PG L ++MGIL
Sbjct: 189 KIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMGIL 248

Query: 245 VLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELSTDNV 304
           VL+LGQDLPDGNL++LQK G V+KDKFS + W+AV NYRTWI  +LYG SMG+EL+ +NV
Sbjct: 249 VLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTINNV 308

Query: 305 IAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIFKAF 364
           I+ YF+DR                                                    
Sbjct: 309 ISGYFYDR---------------------------------------------------- 368

Query: 365 RPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCG 424
                  F+LKL TAGI+AA+FGMAN +ARPFGGYASDVAAR FGMRGRLWTLWI QT G
Sbjct: 369 -------FNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQTVG 428

Query: 425 GVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 484
            +FC+WLG+A+ LP AI+AM+LFSIG QAACGA FG+ PF+SRRSLG+ISGLTGAGGNFG
Sbjct: 429 ALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNFG 488

Query: 485 SGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKSTEEF 544
           SGLTQL+FFS+ ++ST  GL LMG+M V CTLPV+ +HFPQWGSMFL P+   E+S EE+
Sbjct: 489 SGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQEEY 539

Query: 545 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASA--PTPPNTTPSHV 590
           YYGSEWTE+EK++GLH+ SIKFAENSRSERGR+VA A  PTP N TPSHV
Sbjct: 549 YYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 539

BLAST of CcUC03G045010 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 746.9 bits (1927), Expect = 1.2e-215
Identity = 382/593 (64.42%), Postives = 447/593 (75.38%), Query Frame = 0

Query: 5   EGSPGSSMHGVTGREQSFAFSV------ASPIVPTDTTAKFALPVDSEHKAKVFKVWSLA 64
           +GS G+S+HGVT REQ F+FSV      +  +   D TAKFALPVDSEH+AKVFK  S A
Sbjct: 9   DGSIGTSLHGVTAREQVFSFSVQEDVPSSQAVRTNDPTAKFALPVDSEHRAKVFKPLSFA 68

Query: 65  NPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVM 124
            PHMR FHL WISFFTCF+STFAAAPLVP+IRDNL+LTK DIGNAGV SVSG+IFSRL M
Sbjct: 69  KPHMRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTKTDIGNAGVASVSGAIFSRLAM 128

Query: 125 GAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTM 184
           GAVCDLLG RYG AF +ML+AP VF MSFV++A  Y+AVRFMIGF LATFVSCQYW S M
Sbjct: 129 GAVCDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAVRFMIGFCLATFVSCQYWTSVM 188

Query: 185 FNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGSLHILM 244
           F  +IIGLVNG A GWG+MGGG TQL+MP+++ VI+  GATPFTAWR AFFIPG L I+M
Sbjct: 189 FTGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTGATPFTAWRFAFFIPGILQIVM 248

Query: 245 GILVLTLGQDLPDGNLTSLQKKGNVAKDKFSNILWYAVTNYRTWIFVLLYGFSMGVELST 304
           GILVLTLGQDLPDGNL++LQK G V+KDKFS + W+AV NYRTWI  +LYGFSMGVEL+ 
Sbjct: 249 GILVLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFMLYGFSMGVELTI 308

Query: 305 DNVIAEYFFDRRKQYYEKPTYPCVAQVHVQIETPFTLTQKYLGFRFSTKGAFFYVSYNIF 364
           +NVI+ YF+DR                                                 
Sbjct: 309 NNVISGYFYDR------------------------------------------------- 368

Query: 365 KAFRPYVLNLFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQ 424
                     F+L LHTAGIIAA+FGMAN  ARPFGGYASDVAAR FGMRGRLW LWILQ
Sbjct: 369 ----------FNLTLHTAGIIAASFGMANFFARPFGGYASDVAARLFGMRGRLWILWILQ 428

Query: 425 TCGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGG 484
           T G +FC+WLG+A+ LP AI+AM+LFS+G QAACGA FG+ PF+SRRSLG+ISGLTGAGG
Sbjct: 429 TVGALFCIWLGRASSLPIAILAMMLFSMGTQAACGALFGVAPFVSRRSLGLISGLTGAGG 488

Query: 485 NFGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVSLVHFPQWGSMFLPPTKHVEKST 544
           NFGSG+TQL+FFS++++ST  GL LMG+M V C+LPV+ +HFPQWGSMFL P++  EKS 
Sbjct: 489 NFGSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVAFIHFPQWGSMFLRPSQDGEKSK 542

Query: 545 EEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRR--VASAPTPPNTTPSHV 590
           EE YYG+EWTE+EK  GLH+ SIKFAENSRSERGR+  +A  PTP   +P+HV
Sbjct: 549 EEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGRKAMLADIPTPETGSPAHV 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895485.15.0e-29187.78high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 h... [more]
XP_031736393.19.4e-29087.27high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP5371... [more]
NP_001274401.13.6e-28987.27high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-af... [more]
AGO64298.14.6e-28986.93high-affinity nitrate transporter NRT2.2 [Cucumis sativus][more]
NP_001269063.26.7e-28886.76high affinity nitrate transporter 2.4-like [Cucumis sativus][more]
Match NameE-valueIdentityDescription
O828114.4e-24272.16High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ... [more]
Q9FJH81.3e-24171.65High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 ... [more]
P0DKG95.6e-22968.71High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKH05.6e-22968.71High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LMZ95.3e-21967.83High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 ... [more]
Match NameE-valueIdentityDescription
A0A3Q9CZ424.5e-29087.27High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 ... [more]
Q6PRP81.7e-28987.27High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3[more]
S4VM422.2e-28986.93High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE... [more]
A0A6J1HIH18.3e-28485.40high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1DHB73.1e-28384.69high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT1G08090.13.1e-24372.16nitrate transporter 2:1 [more]
AT5G60770.19.2e-24371.65nitrate transporter 2.4 [more]
AT1G08100.13.7e-22067.83nitrate transporter 2.2 [more]
AT5G60780.15.4e-21965.42nitrate transporter 2.3 [more]
AT3G45060.11.2e-21564.42high affinity nitrate transporter 2.6 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 69..294
e-value: 1.6E-13
score: 50.3
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 58..254
e-value: 5.8E-21
score: 76.8
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 264..523
e-value: 2.1E-20
score: 75.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 347..523
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 58..308
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 549..589
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 549..572
NoneNo IPR availablePANTHERPTHR23515:SF16HIGH-AFFINITY NITRATE TRANSPORTER 2.1-LIKEcoord: 363..586
NoneNo IPR availablePANTHERPTHR23515:SF16HIGH-AFFINITY NITRATE TRANSPORTER 2.1-LIKEcoord: 1..310
IPR044772Nitrate transporterPANTHERPTHR23515HIGH-AFFINITY NITRATE TRANSPORTER 2.3coord: 363..586
coord: 1..310
IPR044772Nitrate transporterCDDcd17341MFS_NRT2_likecoord: 65..517
e-value: 6.60615E-126
score: 373.51

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC03G045010.1CcUC03G045010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071249 cellular response to nitrate
biological_process GO:0042128 nitrate assimilation
biological_process GO:0015706 nitrate transport
biological_process GO:0031204 posttranslational protein targeting to membrane, translocation
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0071261 Ssh1 translocon complex
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0008320 protein transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity