CcUC03G042340 (gene) Watermelon (PI 537277) v1

BLAST of CcUC03G042340 vs. NCBI nr
Match: XP_038877491.1 (kinetochore protein NUF2 homolog [Benincasa hispida] >XP_038877492.1 kinetochore protein NUF2 homolog [Benincasa hispida])

HSP 1 Score: 768.5 bits (1983), Expect = 3.3e-218
Identity = 412/457 (90.15%), Postives = 428/457 (93.65%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKY+FPRLPRADIA +LAD+QIA VTERDLLHPS DLVSDLYTRLMIYLDFLHEED+GQ
Sbjct: 1   MSKYDFPRLPRADIALLLADYQIAAVTERDLLHPSLDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           VEFAALDQLENPDLHMDSV  MKL N+IKH IASLDCPKKFTLKDLIKPEADR EIFLSA
Sbjct: 61  VEFAALDQLENPDLHMDSVSTMKLLNKIKHVIASLDCPKKFTLKDLIKPEADRTEIFLSA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           MLNFCIHKDAKINLHA VMDELN FGDQQREWEVKISQLNAEI EYNEAREREIPFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMDELNTFGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKKLAREEAK AEK AMQACQEKTGIVEVYSKVSKKMLKHLSLMQTI 
Sbjct: 241 VQSPDKLQRALEEKKLAREEAKNAEKLAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIH 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+EREFKALKVKL+DEEVQDKSLQVKLVELQAKVHQLKDLV QTEK+RDV C+
Sbjct: 301 EQVNSAKSVEREFKALKVKLSDEEVQDKSLQVKLVELQAKVHQLKDLVTQTEKDRDVKCE 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EATKDLN VRSHVES+R DLEQRQRNVEAV MEVD LTS+TNS+R+SSV KQQELLNKCE
Sbjct: 361 EATKDLNGVRSHVESMRHDLEQRQRNVEAVFMEVDVLTSKTNSVRQSSVLKQQELLNKCE 420

Query: 421 SIIKEVHAYANLVGALIEVEAK----EEEEERTPFSG 454
           SIIKE H Y NL+GAL+EVEAK    EEEEERT FSG
Sbjct: 421 SIIKEFHQYTNLIGALMEVEAKEEEEEEEEERTHFSG 457

BLAST of CcUC03G042340 vs. NCBI nr
Match: XP_008465693.1 (PREDICTED: probable kinetochore protein NUF2 [Cucumis melo] >XP_008465694.1 PREDICTED: probable kinetochore protein NUF2 [Cucumis melo] >XP_008465695.1 PREDICTED: probable kinetochore protein NUF2 [Cucumis melo] >XP_016903455.1 PREDICTED: probable kinetochore protein NUF2 [Cucumis melo] >XP_016903456.1 PREDICTED: probable kinetochore protein NUF2 [Cucumis melo])

HSP 1 Score: 736.9 bits (1901), Expect = 1.1e-208
Identity = 394/446 (88.34%), Postives = 416/446 (93.27%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKYEFPRLPRADIA +LAD+QIA VTERDLL PSPDLVSDLYT LMIYLD LHEED+GQ
Sbjct: 1   MSKYEFPRLPRADIALLLADNQIAAVTERDLLQPSPDLVSDLYTHLMIYLDLLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           +EFAALDQLENPDLHM SVP MKLHN+IKH IASLDCPKKFTLKDLIKPEADR EIFLSA
Sbjct: 61  IEFAALDQLENPDLHMVSVPTMKLHNKIKHFIASLDCPKKFTLKDLIKPEADRTEIFLSA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           +LNF IHKDAK+N HA VM+EL+ F DQQREWEVKISQLNAEITEYNEARERE+PFVQEI
Sbjct: 121 ILNFGIHKDAKMNFHAPVMNELDTFADQQREWEVKISQLNAEITEYNEAREREMPFVQEI 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQTIGGLNNQQ+SLRA+IRKLKEKAGEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 181 DAKVKELHQTIGGLNNQQVSLRAAIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKKLAREEAK AEK AMQACQEKTGIVEVYSKVSKKMLKHLSLMQTI 
Sbjct: 241 VQSPDKLQRALEEKKLAREEAKNAEKLAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIH 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+EREFKALKVKL+DEEVQDKSLQVKLVELQAKVHQLKDLV QTEKERDV C+
Sbjct: 301 EQVNSAKSVEREFKALKVKLSDEEVQDKSLQVKLVELQAKVHQLKDLVTQTEKERDVTCE 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EATKDL  VRS VES+R DLEQRQRNVEAV MEVD LTS+TNSI++SSV KQQELLNKCE
Sbjct: 361 EATKDLIAVRSQVESLRHDLEQRQRNVEAVFMEVDVLTSKTNSIKQSSVLKQQELLNKCE 420

Query: 421 SIIKEVHAYANLVGALIEVEAKEEEE 447
           SIIKE H Y NLVGAL+EV+ KEEEE
Sbjct: 421 SIIKEFHQYTNLVGALMEVKTKEEEE 446

BLAST of CcUC03G042340 vs. NCBI nr
Match: XP_004143795.1 (kinetochore protein NUF2 homolog [Cucumis sativus] >KGN51198.1 hypothetical protein Csa_008808 [Cucumis sativus])

HSP 1 Score: 736.5 bits (1900), Expect = 1.4e-208
Identity = 394/446 (88.34%), Postives = 414/446 (92.83%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKYEFPRLPRADI  +LAD+QIA VTERDLL PSPDLVSDLYT LMIYLD LHEED+GQ
Sbjct: 1   MSKYEFPRLPRADIVLLLADNQIAAVTERDLLQPSPDLVSDLYTHLMIYLDLLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           +EFAALDQLENPDLHM SVP MKLHN+IKH IASLDCPKKFTLKDLIKPE DR EIFLSA
Sbjct: 61  IEFAALDQLENPDLHMVSVPTMKLHNKIKHFIASLDCPKKFTLKDLIKPETDRTEIFLSA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           +LNF IHKDAK+N HA VM+EL+ F DQQREWEVK SQLNAEITEYNEARERE+PFVQEI
Sbjct: 121 ILNFGIHKDAKMNFHAPVMNELDTFADQQREWEVKTSQLNAEITEYNEAREREMPFVQEI 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQTIGGLNNQQ+SLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 181 DAKVKELHQTIGGLNNQQVSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKKLAREEAK AEK AMQACQEKTGIVEVYSKVSKKMLKHLSLMQTI 
Sbjct: 241 VQSPDKLQRALEEKKLAREEAKNAEKLAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIH 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+EREFKALKVKL+DEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDV C+
Sbjct: 301 EQVNSAKSVEREFKALKVKLSDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVTCE 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EATKDL  VRS VES+R DLEQRQRNVEAV MEVD LTS+TNSIR+SSV KQQELLNKCE
Sbjct: 361 EATKDLIAVRSQVESLRHDLEQRQRNVEAVFMEVDVLTSKTNSIRQSSVLKQQELLNKCE 420

Query: 421 SIIKEVHAYANLVGALIEVEAKEEEE 447
           SIIKE H Y NLVGAL+EV+ KEEEE
Sbjct: 421 SIIKEFHQYTNLVGALMEVKTKEEEE 446

BLAST of CcUC03G042340 vs. NCBI nr
Match: KAG6588803.1 (Kinetochore protein NUF2-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 707.2 bits (1824), Expect = 9.0e-200
Identity = 374/440 (85.00%), Postives = 406/440 (92.27%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKYEFPRLPRADIA +LA  QIA VTERDLLHP+PDLVSDLYTRLMIYLDFLHEED+GQ
Sbjct: 58  MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 117

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           VEFAAL+Q ENPDLHMDSV  MKL+NR+KH IASLDCPKKFTLKDLIKP+ DR EIFLSA
Sbjct: 118 VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 177

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           MLNFCIHKDAKINLHA VM+ELN   DQQREWEVKISQLNAEI EYNEARERE+PFVQEI
Sbjct: 178 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 237

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 238 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 297

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKKLAREEAK AEK AMQA QEKTGIVEVYSKVS+KMLKHL+ MQTIQ
Sbjct: 298 VQSPDKLQRALEEKKLAREEAKNAEKLAMQAFQEKTGIVEVYSKVSRKMLKHLAQMQTIQ 357

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+E+EFKALK KL+DEEV DKSLQVKLVE +AKV QL+DL RQTEKER+V C+
Sbjct: 358 EQVNSAKSVEKEFKALKAKLSDEEVVDKSLQVKLVEHEAKVRQLEDLRRQTEKEREVKCE 417

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EA K+LN+VR  VES++LDLEQRQ+NVEAVLMEVD LTS+TNS+RES+VHKQQELL+KCE
Sbjct: 418 EAAKELNNVRLEVESMKLDLEQRQKNVEAVLMEVDALTSKTNSLRESNVHKQQELLHKCE 477

Query: 421 SIIKEVHAYANLVGALIEVE 441
           +I+KE H Y N  GAL+EV+
Sbjct: 478 NIMKEFHQYTNSFGALMEVK 497

BLAST of CcUC03G042340 vs. NCBI nr
Match: XP_022927739.1 (probable kinetochore protein NUF2 [Cucurbita moschata] >XP_022927740.1 probable kinetochore protein NUF2 [Cucurbita moschata] >XP_022927741.1 probable kinetochore protein NUF2 [Cucurbita moschata] >KAG7022576.1 NUF2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 707.2 bits (1824), Expect = 9.0e-200
Identity = 374/440 (85.00%), Postives = 406/440 (92.27%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKYEFPRLPRADIA +LA  QIA VTERDLLHP+PDLVSDLYTRLMIYLDFLHEED+GQ
Sbjct: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           VEFAAL+Q ENPDLHMDSV  MKL+NR+KH IASLDCPKKFTLKDLIKP+ DR EIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           MLNFCIHKDAKINLHA VM+ELN   DQQREWEVKISQLNAEI EYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKKLAREEAK AEK AMQA QEKTGIVEVYSKVS+KMLKHL+ MQTIQ
Sbjct: 241 VQSPDKLQRALEEKKLAREEAKNAEKLAMQAFQEKTGIVEVYSKVSRKMLKHLAQMQTIQ 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+E+EFKALK KL+DEEV DKSLQVKLVE +AKV QL+DL RQTEKER+V C+
Sbjct: 301 EQVNSAKSVEKEFKALKAKLSDEEVVDKSLQVKLVEHEAKVRQLEDLRRQTEKEREVKCE 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EA K+LN+VR  VES++LDLEQRQ+NVEAVLMEVD LTS+TNS+RES+VHKQQELL+KCE
Sbjct: 361 EAAKELNNVRLEVESMKLDLEQRQKNVEAVLMEVDALTSKTNSLRESNVHKQQELLHKCE 420

Query: 421 SIIKEVHAYANLVGALIEVE 441
           +I+KE H Y N  GAL+EV+
Sbjct: 421 NIMKEFHQYTNSFGALMEVK 440

BLAST of CcUC03G042340 vs. ExPASy Swiss-Prot
Match: Q8RXJ0 (Kinetochore protein NUF2 homolog OS=Arabidopsis thaliana OX=3702 GN=NUF2 PE=1 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 3.0e-113
Identity = 226/429 (52.68%), Postives = 315/429 (73.43%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MS YE+PRL R+DI + L D QIA VTE DL  P+ D VS+LYTR++IYLD L EE+KGQ
Sbjct: 1   MSAYEYPRLSRSDIITALKDAQIASVTETDLKTPTSDFVSELYTRILIYLDALDEEEKGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           V+F AL+QLENPD H  S+  MKL+ ++K  +  LDCP   + KDL++PE+ R E F+SA
Sbjct: 61  VDFEALEQLENPDHHATSMQAMKLYCKVKDMLEMLDCPLPISFKDLLRPESSRTEFFISA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           +LN+ ++KD+K++L     +EL    +Q+++ E K++QLNAEI E++EA ER++PFVQE+
Sbjct: 121 LLNYGLYKDSKMDLIRPKAEELGLLDEQRKQCEAKVAQLNAEIGEFDEAVERDLPFVQEL 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           +A +++L++ I  LNNQQMSLRA+ +K++EK+ +MD +IS AEF LV++VQENANLRS+I
Sbjct: 181 EANIEQLNKKILELNNQQMSLRATFQKMREKSTQMDNEISKAEFDLVETVQENANLRSQI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQ ALEEKKL   E KKAE+ AM   QEK  I+EV+ K  KK+LK  S +Q I 
Sbjct: 241 VQSPDKLQGALEEKKLVLGETKKAEQSAMVTFQEKAAILEVFEKALKKILKSSSQLQLIN 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQV +AK++E+EFKALK KL+++ V  KSL+ K+VE +  V QL + ++Q EKE+ V   
Sbjct: 301 EQVTNAKTVEKEFKALKDKLSEDGVAYKSLEAKVVERERIVEQLNESLKQLEKEKAVMFD 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           + TK LN+++  VES R +LE RQ NVE+V+  VD  T++TN +R+S   K ++L  K E
Sbjct: 361 DWTKQLNELKVEVESRRRELETRQTNVESVVAMVDDNTAKTNQVRQSGEAKVKKLAAKYE 420

Query: 421 SIIKEVHAY 430
            I+K+ H Y
Sbjct: 421 EIVKQFHEY 429

BLAST of CcUC03G042340 vs. ExPASy Swiss-Prot
Match: P0CP41 (Probable kinetochore protein NUF2 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=NUF2 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.9e-14
Identity = 89/443 (20.09%), Postives = 187/443 (42.21%), Query Frame = 0

Query: 6   FPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYL--------DFLHEED 65
           FP L   DI   LA   I    E DL  P+      +Y  L+  L        +   +  
Sbjct: 15  FPLLTAHDILECLAALDIPAQME-DLTKPTAQSTQSIYGSLLEVLMGASINSIEGPKQAL 74

Query: 66  KGQVEFAALD---------QLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIK 125
            G +E+  L           L   +++ D++  M      +        P  F + DL +
Sbjct: 75  LGMMEYKVLSFRSMKRTQINLSRQEMYSDTLQFMMFFKHCRRLALLCGIP-DFAISDLAR 134

Query: 126 PEADRMEIFLSAMLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNE 185
           P+A+R+   LS ++NF   +D ++   A   + L     +  +   K  +L  +  E   
Sbjct: 135 PDANRLRKVLSGIMNFAKFRDERMQTQARFQENLQKHQKKAVDLRRKTEELETQFQEITA 194

Query: 186 AREREIPFVQEIDAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQ 245
               E P  ++   + + L   +  LN+Q++       +LK++   + E++++   ++ Q
Sbjct: 195 RNAAERPQSEQAQKRNELLKSELLELNSQRLKEVQEYEELKKERQTLLEQVNHNNRIVTQ 254

Query: 246 SVQENANLRSRIVQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKK 305
              +  + +SR+VQSPD+++R + E   A +  K       Q  +E T  +EV   +   
Sbjct: 255 LELQIGSAKSRLVQSPDRIKRHISEMSFAIQSEKAKLASFQQKARELTNRLEVIGALEVD 314

Query: 306 MLKHLSLMQTIQEQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLV 365
           +   + L  +IQ+Q    +  +R   AL+ +L   +++ + L  KL +LQ ++      +
Sbjct: 315 LRGLIDLEHSIQDQRAKTEEAKRSKSALEARLEGRQIESQGLAAKLEQLQRQLQNASHKL 374

Query: 366 RQTEKERDVNCQEATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESS 425
            + E+ R    +   + ++++++  +    +  + Q+  + +L E   L SE  +     
Sbjct: 375 ARQEETRKGMRERGARRIDELKAEYKVRARERGEWQKQRDDLLAEQKELESEMAAFVTKH 434

Query: 426 VHKQQELLNKCESIIKEVHAYAN 432
            ++  E L+   ++ ++   Y N
Sbjct: 435 ENEINEFLHAYWTMRRQAEDYMN 455

BLAST of CcUC03G042340 vs. ExPASy Swiss-Prot
Match: P0CP40 (Probable kinetochore protein NUF2 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=NUF2 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.9e-14
Identity = 89/443 (20.09%), Postives = 187/443 (42.21%), Query Frame = 0

Query: 6   FPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYL--------DFLHEED 65
           FP L   DI   LA   I    E DL  P+      +Y  L+  L        +   +  
Sbjct: 15  FPLLTAHDILECLAALDIPAQME-DLTKPTAQSTQSIYGSLLEVLMGASINSIEGPKQAL 74

Query: 66  KGQVEFAALD---------QLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIK 125
            G +E+  L           L   +++ D++  M      +        P  F + DL +
Sbjct: 75  LGMMEYKVLSFRSMKRTQINLSRQEMYSDTLQFMMFFKHCRRLALLCGIP-DFAISDLAR 134

Query: 126 PEADRMEIFLSAMLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNE 185
           P+A+R+   LS ++NF   +D ++   A   + L     +  +   K  +L  +  E   
Sbjct: 135 PDANRLRKVLSGIMNFAKFRDERMQTQARFQENLQKHQKKAVDLRRKTEELETQFQEITA 194

Query: 186 AREREIPFVQEIDAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQ 245
               E P  ++   + + L   +  LN+Q++       +LK++   + E++++   ++ Q
Sbjct: 195 RNAAERPQSEQAQKRNELLKSELLELNSQRLKEVQEYEELKKERQTLLEQVNHNNRIVTQ 254

Query: 246 SVQENANLRSRIVQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKK 305
              +  + +SR+VQSPD+++R + E   A +  K       Q  +E T  +EV   +   
Sbjct: 255 LELQIGSAKSRLVQSPDRIKRHISEMSFAIQSEKAKLASFQQKARELTNRLEVIGALEVD 314

Query: 306 MLKHLSLMQTIQEQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLV 365
           +   + L  +IQ+Q    +  +R   AL+ +L   +++ + L  KL +LQ ++      +
Sbjct: 315 LRGLIDLEHSIQDQRAKTEEAKRSKSALEARLEGRQIESQGLAAKLEQLQRQLQNASHKL 374

Query: 366 RQTEKERDVNCQEATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESS 425
            + E+ R    +   + ++++++  +    +  + Q+  + +L E   L SE  +     
Sbjct: 375 ARQEETRKGMRERGARRIDELKAEYKVRARERGEWQKQRDDLLAEQKELESEMAAFVTKH 434

Query: 426 VHKQQELLNKCESIIKEVHAYAN 432
            ++  E L+   ++ ++   Y N
Sbjct: 435 ENEINEFLHAYWTMRRQAEDYMN 455

BLAST of CcUC03G042340 vs. ExPASy Swiss-Prot
Match: Q10173 (Kinetochore protein nuf2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nuf2 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.4e-08
Identity = 83/418 (19.86%), Postives = 182/418 (43.54%), Query Frame = 0

Query: 3   KYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQVE 62
           K+ FP L RA+I   + D      T ++L  P+   V  LY   +     L  ++  +  
Sbjct: 4   KHTFPSLKRAEILECI-DGLGIPFTAKELDQPTSKAVIPLYEEFLDLFMGLTRQNLEEKV 63

Query: 63  FAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSAML 122
            +  D +EN ++  +S+     +  +   + ++ C   FT++DL+KP+ +R+++ LSA++
Sbjct: 64  NSLQDSVENFEIIHESLRFTVFYQILSQFMQNI-CFHDFTIQDLLKPDRNRLQLILSAVI 123

Query: 123 NFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEIDA 182
           NF   ++ ++      + +  +  +     + +   L  ++    + +      +++ + 
Sbjct: 124 NFAKLREERLQQFDDDIQKRESLLETYTLLDAQRKDLEEKVLLSQDRKLESEAIIKQNEE 183

Query: 183 KVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRIVQ 242
           + +E+ Q++         +R    +++ +A E+  +  N + L+  +++E   L+S IV 
Sbjct: 184 RNEEMFQSLIEDKRLCSQVRTEYDRIRMEASELKIRYHNVDSLMASTLEEIEKLQSSIVH 243

Query: 243 SPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQEQ 302
           SP+KL+  + +  L  +  +  +             VE+  K SK +   L+ +Q I+  
Sbjct: 244 SPEKLKGKIADTSLRIQNDRSQQ-------------VEL-DKKSKILHTKLNSLQLIEGD 303

Query: 303 VNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQEA 362
           +N+   +  E      KL    V           L +   +L D +   +K+ +   ++ 
Sbjct: 304 LNACLKVLEECLVELDKLEHATV-----------LLSTNQELCDQIEINKKKLEFRKEQL 363

Query: 363 TKDLNDVRSHVESIRLDLEQRQRN--VEAVLMEVDTLTSETNSIRESSVHKQQELLNK 419
            K L++ +      +L+ EQ  RN  +EA    +D +  E   I +    K QE   K
Sbjct: 364 LKQLSNAQE-----KLEHEQHSRNQKLEAAKQRMDNIREEYKVITQERNKKIQETEKK 389

BLAST of CcUC03G042340 vs. ExPASy Swiss-Prot
Match: Q6BPA9 (Probable kinetochore protein NUF2 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=NUF2 PE=3 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 4.5e-08
Identity = 80/366 (21.86%), Postives = 169/366 (46.17%), Query Frame = 0

Query: 68  QLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSAMLNFC-I 127
           Q+E+ D    SV ++ LH R  +   S+     FTL D+++PE  R+   LSA++N+   
Sbjct: 112 QIEDDDDITSSVRLILLH-RGAYEFFSICGVNDFTLMDIMRPEPQRIRRILSAVVNYARF 171

Query: 128 HKDAKINLHALV------MDELNAFGDQQREWEVKISQLNAEITEYNEARE-REIPFVQE 187
            ++  +    LV      +++L    ++      KI+ L  +I E NE  E  +   +++
Sbjct: 172 REENSVECEKLVSISEGNLEQLKVVQNENSRLSNKINDLKYKI-EANETDEGNKKATLKQ 231

Query: 188 IDAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSR 247
           I     +L   +  L   Q  L     K K++   + EK+ +  +L+++S +E   L+S 
Sbjct: 232 ITTYNSKLENELKKLKKNQEILTLEHSKYKDEKRRLIEKLEDHNYLIMESNKELDKLKSY 291

Query: 248 IVQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTI 307
           ++ +P+ L + +E+ K    E              +T + ++ SK +K M   +   Q +
Sbjct: 292 LLSNPEILTKIIEDLKTNLSE-------------YQTNLADLESK-NKNMTISIESFQLV 351

Query: 308 QEQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNC 367
           ++++ +       F+ L+  LND   ++ SL     +   K  + K+    T  + +   
Sbjct: 352 EQELKNL------FRILEEVLNDLTKEETSL-----DKLNKYQEFKEQQNLTLNDLNRQI 411

Query: 368 QEATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEV----DTLTSETNSIRESSVHKQQEL 422
           Q+ T+ LN+    ++ +R    +R    +  L+ +    DTL  E   ++E  ++K+++ 
Sbjct: 412 QQVTRQLNNTEEKIKRLRHQSSERSEASKQKLISLKDGYDTLVKE-RKVKEQELNKKKDF 449

BLAST of CcUC03G042340 vs. ExPASy TrEMBL
Match: A0A1S4E5E4 (probable kinetochore protein NUF2 OS=Cucumis melo OX=3656 GN=LOC103503319 PE=3 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 5.2e-209
Identity = 394/446 (88.34%), Postives = 416/446 (93.27%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKYEFPRLPRADIA +LAD+QIA VTERDLL PSPDLVSDLYT LMIYLD LHEED+GQ
Sbjct: 1   MSKYEFPRLPRADIALLLADNQIAAVTERDLLQPSPDLVSDLYTHLMIYLDLLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           +EFAALDQLENPDLHM SVP MKLHN+IKH IASLDCPKKFTLKDLIKPEADR EIFLSA
Sbjct: 61  IEFAALDQLENPDLHMVSVPTMKLHNKIKHFIASLDCPKKFTLKDLIKPEADRTEIFLSA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           +LNF IHKDAK+N HA VM+EL+ F DQQREWEVKISQLNAEITEYNEARERE+PFVQEI
Sbjct: 121 ILNFGIHKDAKMNFHAPVMNELDTFADQQREWEVKISQLNAEITEYNEAREREMPFVQEI 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQTIGGLNNQQ+SLRA+IRKLKEKAGEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 181 DAKVKELHQTIGGLNNQQVSLRAAIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKKLAREEAK AEK AMQACQEKTGIVEVYSKVSKKMLKHLSLMQTI 
Sbjct: 241 VQSPDKLQRALEEKKLAREEAKNAEKLAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIH 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+EREFKALKVKL+DEEVQDKSLQVKLVELQAKVHQLKDLV QTEKERDV C+
Sbjct: 301 EQVNSAKSVEREFKALKVKLSDEEVQDKSLQVKLVELQAKVHQLKDLVTQTEKERDVTCE 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EATKDL  VRS VES+R DLEQRQRNVEAV MEVD LTS+TNSI++SSV KQQELLNKCE
Sbjct: 361 EATKDLIAVRSQVESLRHDLEQRQRNVEAVFMEVDVLTSKTNSIKQSSVLKQQELLNKCE 420

Query: 421 SIIKEVHAYANLVGALIEVEAKEEEE 447
           SIIKE H Y NLVGAL+EV+ KEEEE
Sbjct: 421 SIIKEFHQYTNLVGALMEVKTKEEEE 446

BLAST of CcUC03G042340 vs. ExPASy TrEMBL
Match: A0A0A0KNL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G488740 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 6.7e-209
Identity = 394/446 (88.34%), Postives = 414/446 (92.83%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKYEFPRLPRADI  +LAD+QIA VTERDLL PSPDLVSDLYT LMIYLD LHEED+GQ
Sbjct: 1   MSKYEFPRLPRADIVLLLADNQIAAVTERDLLQPSPDLVSDLYTHLMIYLDLLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           +EFAALDQLENPDLHM SVP MKLHN+IKH IASLDCPKKFTLKDLIKPE DR EIFLSA
Sbjct: 61  IEFAALDQLENPDLHMVSVPTMKLHNKIKHFIASLDCPKKFTLKDLIKPETDRTEIFLSA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           +LNF IHKDAK+N HA VM+EL+ F DQQREWEVK SQLNAEITEYNEARERE+PFVQEI
Sbjct: 121 ILNFGIHKDAKMNFHAPVMNELDTFADQQREWEVKTSQLNAEITEYNEAREREMPFVQEI 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQTIGGLNNQQ+SLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 181 DAKVKELHQTIGGLNNQQVSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKKLAREEAK AEK AMQACQEKTGIVEVYSKVSKKMLKHLSLMQTI 
Sbjct: 241 VQSPDKLQRALEEKKLAREEAKNAEKLAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIH 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+EREFKALKVKL+DEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDV C+
Sbjct: 301 EQVNSAKSVEREFKALKVKLSDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVTCE 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EATKDL  VRS VES+R DLEQRQRNVEAV MEVD LTS+TNSIR+SSV KQQELLNKCE
Sbjct: 361 EATKDLIAVRSQVESLRHDLEQRQRNVEAVFMEVDVLTSKTNSIRQSSVLKQQELLNKCE 420

Query: 421 SIIKEVHAYANLVGALIEVEAKEEEE 447
           SIIKE H Y NLVGAL+EV+ KEEEE
Sbjct: 421 SIIKEFHQYTNLVGALMEVKTKEEEE 446

BLAST of CcUC03G042340 vs. ExPASy TrEMBL
Match: A0A6J1EIG6 (probable kinetochore protein NUF2 OS=Cucurbita moschata OX=3662 GN=LOC111434552 PE=3 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 4.4e-200
Identity = 374/440 (85.00%), Postives = 406/440 (92.27%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKYEFPRLPRADIA +LA  QIA VTERDLLHP+PDLVSDLYTRLMIYLDFLHEED+GQ
Sbjct: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           VEFAAL+Q ENPDLHMDSV  MKL+NR+KH IASLDCPKKFTLKDLIKP+ DR EIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           MLNFCIHKDAKINLHA VM+ELN   DQQREWEVKISQLNAEI EYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKKLAREEAK AEK AMQA QEKTGIVEVYSKVS+KMLKHL+ MQTIQ
Sbjct: 241 VQSPDKLQRALEEKKLAREEAKNAEKLAMQAFQEKTGIVEVYSKVSRKMLKHLAQMQTIQ 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+E+EFKALK KL+DEEV DKSLQVKLVE +AKV QL+DL RQTEKER+V C+
Sbjct: 301 EQVNSAKSVEKEFKALKAKLSDEEVVDKSLQVKLVEHEAKVRQLEDLRRQTEKEREVKCE 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EA K+LN+VR  VES++LDLEQRQ+NVEAVLMEVD LTS+TNS+RES+VHKQQELL+KCE
Sbjct: 361 EAAKELNNVRLEVESMKLDLEQRQKNVEAVLMEVDALTSKTNSLRESNVHKQQELLHKCE 420

Query: 421 SIIKEVHAYANLVGALIEVE 441
           +I+KE H Y N  GAL+EV+
Sbjct: 421 NIMKEFHQYTNSFGALMEVK 440

BLAST of CcUC03G042340 vs. ExPASy TrEMBL
Match: A0A6J1JNW1 (probable kinetochore protein NUF2 OS=Cucurbita maxima OX=3661 GN=LOC111486390 PE=3 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 1.3e-199
Identity = 373/440 (84.77%), Postives = 405/440 (92.05%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKYEFPRLPRADI  +LA  QIA VTERDLLHP+PDLVSDLYTRLMIYLDFLHEED+GQ
Sbjct: 1   MSKYEFPRLPRADIVLLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           VEFAAL+Q ENPDLHMDSV  MKL+NR+KH IASLDCPKKFTLKDLIKP+ DR EIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           MLNFCIHKDAKINLHA VM+ELN   DQQREWEVKISQLNAEI EYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKKLAREEAK AEK AMQA QEKTGIVEVYSKVS+KMLKHL+ MQTIQ
Sbjct: 241 VQSPDKLQRALEEKKLAREEAKNAEKLAMQAFQEKTGIVEVYSKVSRKMLKHLAQMQTIQ 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+E+EFKALK KL+DEEV DKSLQVKLVE +AKV QL+DL RQTEKERDV C+
Sbjct: 301 EQVNSAKSVEKEFKALKAKLSDEEVVDKSLQVKLVEHEAKVRQLEDLRRQTEKERDVKCE 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EA K+LN+VR  VES++LDLEQRQ+NVEAVLMEVD +TS+TNS+RES+VHKQQELL+KCE
Sbjct: 361 EAAKELNNVRLEVESMKLDLEQRQKNVEAVLMEVDAVTSKTNSMRESNVHKQQELLHKCE 420

Query: 421 SIIKEVHAYANLVGALIEVE 441
           +I+KE H Y N  GAL+EV+
Sbjct: 421 NIMKEFHQYTNSFGALMEVK 440

BLAST of CcUC03G042340 vs. ExPASy TrEMBL
Match: A0A6J1DWL1 (probable kinetochore protein NUF2 OS=Momordica charantia OX=3673 GN=LOC111024175 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.6e-192
Identity = 365/440 (82.95%), Postives = 394/440 (89.55%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MSKY   RLP ADIA +LA  QIA VTE DLLHPSPDLVSDLYTRL+IYLDFLHEED+GQ
Sbjct: 1   MSKYGLLRLPPADIALLLAQFQIAAVTEGDLLHPSPDLVSDLYTRLLIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           VEFAALDQLENPDLHMDSV IMKL+ ++KH IASLDC KKFTLKDLIKP+ DR EIFLS 
Sbjct: 61  VEFAALDQLENPDLHMDSVRIMKLNLKLKHVIASLDCSKKFTLKDLIKPDTDRTEIFLST 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LHA +++E+N  GDQQREWEVKISQLNAEI EYNEAREREIPFVQ+I
Sbjct: 121 MLNFCIHKDAKIVLHAPIINEVNTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQDI 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           DAKVKELHQ IGGLNNQQMSLRASIRKLKEK GEMDEKISNAEFLLVQSVQENANLRS+I
Sbjct: 181 DAKVKELHQIIGGLNNQQMSLRASIRKLKEKVGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQRALEEKK AREE K AE+ AMQA QEKTGIVEVYSKVS+KM KHL+ M TIQ
Sbjct: 241 VQSPDKLQRALEEKKSAREETKNAERLAMQAFQEKTGIVEVYSKVSRKMTKHLAQMHTIQ 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQVNSAKS+E+EFKALK KL+DEE+ DKSLQVK+VELQAKV QL+DL RQTEK RDV C+
Sbjct: 301 EQVNSAKSVEKEFKALKAKLSDEELLDKSLQVKMVELQAKVQQLEDLRRQTEKGRDVKCE 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           EATKDLN+VR  VES+RLDLEQRQ NVEAVLMEVD +TS+TNSIRESSVHKQQELL+KCE
Sbjct: 361 EATKDLNNVRLEVESMRLDLEQRQSNVEAVLMEVDAVTSKTNSIRESSVHKQQELLHKCE 420

Query: 421 SIIKEVHAYANLVGALIEVE 441
            IIKE H Y N VGAL+EVE
Sbjct: 421 DIIKEFHQYTNSVGALMEVE 440

BLAST of CcUC03G042340 vs. TAIR 10
Match: AT1G61000.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: mitosis; LOCATED IN: plasma membrane; EXPRESSED IN: cultured cell; CONTAINS InterPro DOMAIN/s: Kinetochore protein Nuf2 (InterPro:IPR005549); Has 50972 Blast hits to 29793 proteins in 2070 species: Archae - 902; Bacteria - 7404; Metazoa - 23628; Fungi - 4156; Plants - 2312; Viruses - 158; Other Eukaryotes - 12412 (source: NCBI BLink). )

HSP 1 Score: 410.2 bits (1053), Expect = 2.1e-114
Identity = 226/429 (52.68%), Postives = 315/429 (73.43%), Query Frame = 0

Query: 1   MSKYEFPRLPRADIASILADHQIAVVTERDLLHPSPDLVSDLYTRLMIYLDFLHEEDKGQ 60
           MS YE+PRL R+DI + L D QIA VTE DL  P+ D VS+LYTR++IYLD L EE+KGQ
Sbjct: 1   MSAYEYPRLSRSDIITALKDAQIASVTETDLKTPTSDFVSELYTRILIYLDALDEEEKGQ 60

Query: 61  VEFAALDQLENPDLHMDSVPIMKLHNRIKHAIASLDCPKKFTLKDLIKPEADRMEIFLSA 120
           V+F AL+QLENPD H  S+  MKL+ ++K  +  LDCP   + KDL++PE+ R E F+SA
Sbjct: 61  VDFEALEQLENPDHHATSMQAMKLYCKVKDMLEMLDCPLPISFKDLLRPESSRTEFFISA 120

Query: 121 MLNFCIHKDAKINLHALVMDELNAFGDQQREWEVKISQLNAEITEYNEAREREIPFVQEI 180
           +LN+ ++KD+K++L     +EL    +Q+++ E K++QLNAEI E++EA ER++PFVQE+
Sbjct: 121 LLNYGLYKDSKMDLIRPKAEELGLLDEQRKQCEAKVAQLNAEIGEFDEAVERDLPFVQEL 180

Query: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSRI 240
           +A +++L++ I  LNNQQMSLRA+ +K++EK+ +MD +IS AEF LV++VQENANLRS+I
Sbjct: 181 EANIEQLNKKILELNNQQMSLRATFQKMREKSTQMDNEISKAEFDLVETVQENANLRSQI 240

Query: 241 VQSPDKLQRALEEKKLAREEAKKAEKFAMQACQEKTGIVEVYSKVSKKMLKHLSLMQTIQ 300
           VQSPDKLQ ALEEKKL   E KKAE+ AM   QEK  I+EV+ K  KK+LK  S +Q I 
Sbjct: 241 VQSPDKLQGALEEKKLVLGETKKAEQSAMVTFQEKAAILEVFEKALKKILKSSSQLQLIN 300

Query: 301 EQVNSAKSIEREFKALKVKLNDEEVQDKSLQVKLVELQAKVHQLKDLVRQTEKERDVNCQ 360
           EQV +AK++E+EFKALK KL+++ V  KSL+ K+VE +  V QL + ++Q EKE+ V   
Sbjct: 301 EQVTNAKTVEKEFKALKDKLSEDGVAYKSLEAKVVERERIVEQLNESLKQLEKEKAVMFD 360

Query: 361 EATKDLNDVRSHVESIRLDLEQRQRNVEAVLMEVDTLTSETNSIRESSVHKQQELLNKCE 420
           + TK LN+++  VES R +LE RQ NVE+V+  VD  T++TN +R+S   K ++L  K E
Sbjct: 361 DWTKQLNELKVEVESRRRELETRQTNVESVVAMVDDNTAKTNQVRQSGEAKVKKLAAKYE 420

Query: 421 SIIKEVHAY 430
            I+K+ H Y
Sbjct: 421 EIVKQFHEY 429