CcUC02G033240 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC02G033240
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	Miraculin-like
Location	CicolChr02: 28927331 .. 28927960 (+)
RNA-Seq Expression	CcUC02G033240
Synteny	CcUC02G033240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTACACAAATTAAATTCTCAAGTACTTTGAAGAATATCTGATGATGATGCTGCATAAATCACTTGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGAGGTGTCGAGTACTACATCTCGCCTGCAATTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTGTGAACATTGTGTTTCAAGCATTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCCGCGGAGTCTGACACCGGGAGGAGGTTCATGGGGATCGGGAGCGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

mRNA sequence

GTTTACACAAATTAAATTCTCAAGTACTTTGAAGAATATCTGATGATGATGCTGCATAAATCACTTGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGAGGTGTCGAGTACTACATCTCGCCTGCAATTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTGTGAACATTGTGTTTCAAGCATTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCCGCGGAGTCTGACACCGGGAGGAGGTTCATGGGGATCGGGAGCGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Coding sequence (CDS)

ATGATGATGCTGCATAAATCACTTGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGAGGTGTCGAGTACTACATCTCGCCTGCAATTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTGTGAACATTGTGTTTCAAGCATTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCCGCGGAGTCTGACACCGGGAGGAGGTTCATGGGGATCGGGAGCGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Protein sequence

MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRVDAAESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA

Homology

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BLAST of CcUC02G033240 vs. NCBI nr
Match: KAA0063690.1 (miraculin-like [Cucumis melo var. makuwa] >TYK18337.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 370.9 bits (951), Expect = 6.6e-99
Identity = 178/195 (91.28%), Postives = 185/195 (94.87%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DT+GQPLRRGVEYYISPAITDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P  AGKDIIDEGTS+NIVF+ALSTC TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRV 120

Query: 121 DAAESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DA ESDTGRRF+GIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 LALDGEAFPFEFVKA 196
           +ALDG+AFPFEF+KA
Sbjct: 181 VALDGDAFPFEFIKA 195

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BLAST of CcUC02G033240 vs. NCBI nr
Match: KGN60910.2 (hypothetical protein Csa_023447 [Cucumis sativus])

HSP 1 Score: 367.9 bits (943), Expect = 5.6e-98
Identity = 176/195 (90.26%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DT+GQPL+RGVEYYISPAITDVGGNLTLKS
Sbjct: 170 MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 229

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P   G DIIDEGTS+NIVFQALSTC+TSTQWRV
Sbjct: 230 RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 289

Query: 121 DAAESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DA ESDTGRRF+GIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 290 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 349

Query: 181 LALDGEAFPFEFVKA 196
           +ALDG+AFPFEF+KA
Sbjct: 350 VALDGDAFPFEFIKA 364

[Close]

BLAST of CcUC02G033240 vs. NCBI nr
Match: KAA0063689.1 (miraculin-like [Cucumis melo var. makuwa] >TYK18338.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 367.9 bits (943), Expect = 5.6e-98
Identity = 176/193 (91.19%), Postives = 183/193 (94.82%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRS 62
           MLHKSLAIFGYACLFMAITS AQ PPV DT+GQPLRRGVEYYISPAITDVGGNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRVDA 122
           NAPCPLFVGQEPVTSTNIGLPVTF P  AGKDIIDEGTS+NIVF+ALSTC TSTQWRVDA
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 123 AESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLA 182
            ESDTGRRF+GIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+A
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 183 LDGEAFPFEFVKA 196
           LDG+AFPFEF+KA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of CcUC02G033240 vs. NCBI nr
Match: XP_004139192.1 (miraculin [Cucumis sativus])

HSP 1 Score: 367.9 bits (943), Expect = 5.6e-98
Identity = 176/195 (90.26%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DT+GQPL+RGVEYYISPAITDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P   G DIIDEGTS+NIVFQALSTC+TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120

Query: 121 DAAESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DA ESDTGRRF+GIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 LALDGEAFPFEFVKA 196
           +ALDG+AFPFEF+KA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of CcUC02G033240 vs. NCBI nr
Match: XP_038888526.1 (kunitz type trypsin inhibitor 106-like [Benincasa hispida])

HSP 1 Score: 360.9 bits (925), Expect = 6.8e-96
Identity = 172/195 (88.21%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DTD QPLRRGVEYYI PAITDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTDSQPLRRGVEYYIKPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTFTPI+AG+DII+E  S+NIVF+ALSTC+TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIVAGEDIIEESRSLNIVFEALSTCVTSTQWRV 120

Query: 121 DAAESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           D AESDTGRRF+GIG++DGP+GIF I R+NGVYNIVWCPAMMGRPRCG AGILVENGVRL
Sbjct: 121 DEAESDTGRRFVGIGNDDGPSGIFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVENGVRL 180

Query: 181 LALDGEAFPFEFVKA 196
           LALDG+AFPFEFVKA
Sbjct: 181 LALDGDAFPFEFVKA 195

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BLAST of CcUC02G033240 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 7.5e-29
Identity = 81/205 (39.51%), Postives = 110/205 (53.66%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKS 60
           M +  ++L I  + CLF+  T+IAQF  V DT G+P+    EY+I P IT+ GG  T+ S
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVS 62

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIID--EGTSVNIVFQALSTCITSTQW 120
           R N  CPL VG E +T    GL V FTP     D  D      + I FQA S+C+ ST+W
Sbjct: 63  R-NESCPLHVGLE-LTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEW 122

Query: 121 RVDAAESDTGRRFMGIGSEDGPAGIFG------ISRDNGVYNIVWCPAMM---GRPRCGR 180
           R+   ++ +GRR +  G++    G +G       +   G+YNI WCP  +    +  CG 
Sbjct: 123 RLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGT 182

Query: 181 AGILVENGVRLLALDGEAFPFEFVK 195
             +L ENG  LLALDG   P  F K
Sbjct: 183 VDMLNENGKILLALDGGPLPLVFQK 203

[Close]

BLAST of CcUC02G033240 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.3e-28
Identity = 76/205 (37.07%), Postives = 112/205 (54.63%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKS 60
           M  +  ++ I     L +  TSIAQF  V DT G+P+    +Y+I PAIT  GG+LTL +
Sbjct: 1   MSTISFTIFILANVWLLVVTTSIAQF--VIDTSGEPVENDEDYFIRPAITGNGGSLTLVT 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGK--DIIDEGTSVNIVFQALSTCITSTQW 120
           R++  CP  VG +P      G  V  +P ++ +  D +  G  + ++FQA ++C  ST+W
Sbjct: 61  RNS--CPFNVGLDP--DAPQGFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEW 120

Query: 121 RVDAAESDTGRRFMGIGSEDGPAGIFG------ISRDNGVYNIVWCPAMM---GRPRCGR 180
           R+   ++ TGRRF+  G +D   G +G       +   G++NI WCP  +    +  CG 
Sbjct: 121 RLGERDATTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGT 180

Query: 181 AGILVENGVRLLALDGEAFPFEFVK 195
            GI+ ENG  LLALDG A P  F K
Sbjct: 181 VGIVRENGKILLALDGSALPVAFQK 199

[Close]

BLAST of CcUC02G033240 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.4e-27
Identity = 81/204 (39.71%), Postives = 108/204 (52.94%), Query Frame = 0


Query: 3   MLHKSLAIF--GYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKS 62
           M  +SL IF   +  L MA TSIAQF  V DT G+P+    EY+I PAIT  GG  TL +
Sbjct: 1   MSTRSLTIFILAHVWLLMATTSIAQF--VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT 60

Query: 63  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDE--GTSVNIVFQALSTCITSTQW 122
             N PCPL VG +    T +G+ V FTP     D  D      + + F   ++C  ST W
Sbjct: 61  -GNGPCPLHVGLDNTEGT-LGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDW 120

Query: 123 RVDAAESDTGRRFMGIGSEDG---PAGIFGI--SRDNGVYNIVWCP---AMMGRPRCGRA 182
           R+   ++ +GRR +  G ++G       F I  ++  G YNI WCP       + +CG  
Sbjct: 121 RLGEKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTV 180

Query: 183 GILVENGVRLLALDGEAFPFEFVK 195
           G++ ENG  LLALDG+A P  F K
Sbjct: 181 GVIRENGKNLLALDGDALPVVFQK 200

[Close]

BLAST of CcUC02G033240 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 99.4 bits (246), Expect = 4.9e-20
Identity = 66/184 (35.87%), Postives = 90/184 (48.91%), Query Frame = 0


Query: 28  PVFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGL----P 87
           PV D DG+ LR G  YYI P + D GG LT+   +  P   FV    V  T   +    P
Sbjct: 35  PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVS--ATTPNGTFVCPPRVVQTRKEVDHDRP 94

Query: 88  VTFTPIMAGKDIIDEGTSVNIVFQALSTC--ITSTQWRVDAAESDTGRRFMGIGSEDGPA 147
           + F P    +D++   T +NI F A   C   +ST WR+D  +  TG+ F+ IG   G  
Sbjct: 95  LAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNP 154

Query: 148 GIFGISR--------DNGVYNIVWCPAMMG--RPRCGRAGILV-ENGVRLLALDGEAFPF 195
           G   IS          +G Y +V+CP + G  + +CG  GI + + G R LAL  + F F
Sbjct: 155 GPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAF 214

[Close]

BLAST of CcUC02G033240 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 8.0e-15
Identity = 53/143 (37.06%), Postives = 69/143 (48.25%), Query Frame = 0


Query: 27  PPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 86
           PPV DTDG  LR    YY+  A    GG LT+       CPLFV Q+P    + G PV  
Sbjct: 26  PPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHDGFPVRI 85

Query: 87  TP--IMAGKDIIDEGTSVNIVFQALSTCITSTQWRVDAAESDTGRRFMGIG-----SEDG 146
           TP  +     II   T V I F+A +TC+ ST+W +D +E   GRR +  G     S  G
Sbjct: 86  TPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHID-SELAAGRRHVITGPVKDPSPSG 145

Query: 147 PAGIFGISRDNGV----YNIVWC 159
               F I + +G     Y ++ C
Sbjct: 146 RENAFRIEKYSGAEVHEYKLMSC 166

[Close]

BLAST of CcUC02G033240 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.2e-99
Identity = 178/195 (91.28%), Postives = 185/195 (94.87%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DT+GQPLRRGVEYYISPAITDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P  AGKDIIDEGTS+NIVF+ALSTC TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRV 120

Query: 121 DAAESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DA ESDTGRRF+GIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 LALDGEAFPFEFVKA 196
           +ALDG+AFPFEF+KA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of CcUC02G033240 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.7e-98
Identity = 176/193 (91.19%), Postives = 183/193 (94.82%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRS 62
           MLHKSLAIFGYACLFMAITS AQ PPV DT+GQPLRRGVEYYISPAITDVGGNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRVDA 122
           NAPCPLFVGQEPVTSTNIGLPVTF P  AGKDIIDEGTS+NIVF+ALSTC TSTQWRVDA
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 123 AESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLA 182
            ESDTGRRF+GIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+A
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 183 LDGEAFPFEFVKA 196
           LDG+AFPFEF+KA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of CcUC02G033240 vs. ExPASy TrEMBL
Match: A0A0A0LIK2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.5e-88
Identity = 159/178 (89.33%), Postives = 167/178 (93.82%), Query Frame = 0


Query: 18  MAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 77
           MAITS AQ PPV DT+GQPL+RGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 78  TNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRVDAAESDTGRRFMGIGSE 137
           TNIGLPVTF P   G DIIDEGTS+NIVFQALSTC+TSTQWRVDA ESDTGRRF+GIG E
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 138 DGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA 196
           DGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+ALDG+AFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]

BLAST of CcUC02G033240 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.4e-85
Identity = 153/193 (79.27%), Postives = 169/193 (87.56%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRS 62
           MLH SLAIF +  +FMAITS AQ PPV DTDGQPLRRGVEYYI PAITDV GNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRVDA 122
           NAPCPL+VGQEP+ STNIGLPVTFTPI+AG+DII+E    N+VFQA STCITSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLA 182
            ES TGRRF+G+G+++GP GIF I R+NGVYNIVWCP M+GRPRCG AGILVE+GVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGEAFPFEFVKA 196
           LDG AFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of CcUC02G033240 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 5.0e-84
Identity = 151/193 (78.24%), Postives = 168/193 (87.05%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRS 62
           MLH SLAIF Y  +F+AITS AQ PPV DTDGQPLRRGVEYYI PAIT+V GNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRVDA 122
           NAPCPL+VGQEPV STNIGLPVTFTPI+AG+DII+E    N+ FQA STCITSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFMGIGSEDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLA 182
            ES TGRRF+G+G+++GP GIF I R+NGVYNIVWCP M+GRPRCG AGILVE+GVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGEAFPFEFVKA 196
           LDG AFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of CcUC02G033240 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-12
Identity = 60/199 (30.15%), Postives = 86/199 (43.22%), Query Frame = 0


Query: 8   LAIFGYACLFMA---ITSIAQFPPVFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRSNA 67
           L IF    +F++   +T+ A   PV D +G+ L  GV YYI P I   GG LT+ +    
Sbjct: 5   LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64

Query: 68  PCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSVNIVFQALSTCITSTQWRVDAAE 127
            CP  V Q+     + GLPV F+P    +  I   T VNI F   S       W +   +
Sbjct: 65  TCPTSVIQDQF-EVSQGLPVKFSPYDKSR-TIPVSTDVNIKFSPTSI------WELANFD 124

Query: 128 SDTGRRFMGIGSEDGPAG------IFGISRDNGVYNIVWCPAMMG--RPRCGRAGILVEN 187
             T + F+     +G  G       F I +    Y I +CP +    +  C   G+ V++
Sbjct: 125 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 184

Query: 188 GVRLLALDGEAFPFEFVKA 196
           G R LAL        F +A
Sbjct: 185 GKRRLALSDVPLKVMFKRA 195

[Close]

BLAST of CcUC02G033240 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 70.1 bits (170), Expect = 2.2e-12
Identity = 52/174 (29.89%), Postives = 85/174 (48.85%), Query Frame = 0


Query: 29  VFDTDGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTN---IGLPVT 88
           V D  G P++  V+YYI PA    GG L   +R+ +   L +  + V S++    GLPVT
Sbjct: 32  VLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLNLDIVQSSSPFVSGLPVT 91

Query: 89  FTPIMAGKDIIDEGTSVNIVFQA-LSTCITSTQWRVDAAESDTGRRFMGIGSEDGPAGI- 148
           F+P+      +    S+N+ F + +  C  S  WR+D +     + F+ IG + G     
Sbjct: 92  FSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHS-VQLRKSFVSIGGQKGKGNSW 151

Query: 149 FGISRDNGVYNIVWCPAMMGRPRCGRAGILV-ENGVRLLALD-GEAFPFEFVKA 196
           F I  D   Y +++CP +     C    + + ++GVR L L   ++F  +F KA
Sbjct: 152 FQIQEDGDAYKLMYCP-ISSIVACINVSLEIDDHGVRRLVLSTDQSFVVKFQKA 203

The following BLAST results are available for this feature:

BLAST of CcUC02G033240 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0063690.1	6.6e-99	91.28	miraculin-like [Cucumis melo var. makuwa] >TYK18337.1 miraculin-like [Cucumis me...	[more]
KGN60910.2	5.6e-98	90.26	hypothetical protein Csa_023447 [Cucumis sativus]	[more]
KAA0063689.1	5.6e-98	91.19	miraculin-like [Cucumis melo var. makuwa] >TYK18338.1 miraculin-like [Cucumis me...	[more]
XP_004139192.1	5.6e-98	90.26	miraculin [Cucumis sativus]	[more]
XP_038888526.1	6.8e-96	88.21	kunitz type trypsin inhibitor 106-like [Benincasa hispida]	[more]

BLAST of CcUC02G033240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV1	7.5e-29	39.51	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
G7LCV7	8.3e-28	37.07	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
A0A072TH68	1.4e-27	39.71	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
P13087	4.9e-20	35.87	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P07596	8.0e-15	37.06	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of CcUC02G033240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D468	3.2e-99	91.28	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]
A0A5A7VDV8	2.7e-98	91.19	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]
A0A0A0LIK2	1.5e-88	89.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1	[more]
A0A6J1G3G3	3.4e-85	79.27	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	5.0e-84	78.24	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]

BLAST of CcUC02G033240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	1.7e-12	30.15	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73325.1	2.2e-12	29.89	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 28..195 e-value: 4.4E-29 score: 112.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 29..194 e-value: 5.7E-40 score: 137.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 4..194
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 29..45
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 28..194 e-value: 7.76947E-39 score: 129.384
None	No IPR available	GENE3D	2.80.10.50		coord: 24..195 e-value: 2.6E-46 score: 159.5
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 4..194
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 27..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC02G033240.1	CcUC02G033240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0003953	NAD+ nucleosidase activity