CcUC02G025100 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC02G025100
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionTocopherol O-methyltransferase
LocationCicolChr02: 9405901 .. 9410298 (-)
RNA-Seq ExpressionCcUC02G025100
SyntenyCcUC02G025100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACTCTTTCCAAAGAAAAAAGAAAAAGGAAATAACGTCTTCTCCTTTCTGTATCTTCTCTCTCAGCATCTCTGTGTTGCTTTACGTTCCCAACACATTGGCATCGGCCATTGCTCCCTTCTATATATTATTACCAACAATGAGCTTCACCGCTGCAACTCCAATCCATACGTCCTCGTATGCTCCTTCCTCTCTTCATTCCAATCGCTTCGACCCCACAATCCAATCTCCCATCCCTCTCCGCCGTTCCTTTTCTTCTTTCACCCCCCGCCGCACCTCTCCTCTGCTTGCTGCCGCTGCGACGACGACCAAGACGGTCCCCGACCAGATGGACTCCGATCGCCTTCAGAAAGGAATCGCCGAGTTCTACGATGAATCTTCGGGGCTTTGGGAGAGTGTTTGGGGCGAACATATGCACCATGGATTTTACGATCCTGATTCTTCCGTTTCCCTCTCCGATCACCGCGCTGCGCAAATCCGGATGATCGACGAGACGCTCCGGTTCGCAGGCGTGGAGGCGGCGATGGCGGCGGTGGACGTTGGGTGTGGGATTGGGGGAAGTTCTAGGTATTTGGCGAGGAAGTTTGGGGCGAAATGTAGAGGTATAACTTTGAGCCCTGTTCAAGCTAAAAGGGCTCAAGCGATTGCTGCGGCTGAAGGATTAAGTGATAAGGTGTGTACGGTCTATGAATCCTCTGTTCTTATTTGTTCTTACTCCGTTTATCCTTTTTGTTTTTTGTGTTTTTCTTTGAAGATTTTCCGTTGTGCATTCATTTTTAGACATTTTCTTTGCTTTGATACTAAACTTGAACATTAGTCCTCAATCAAATAATACAACCAGAATAATGAGTACCTTCTTGGTTAGTCCTTTCCAAGTTTGGTTGCGGAAATGGTTAAAAAAATTGCCCACACAATTACAACATTCAAGACTTCTTTCTAACCATTTTAAATTTGATCTATTTTAATTAAATCGTTAATAGTTTGAAATGCTTGTTAAAAACCGGAAGAAAATGGTGAAGCCAAGAATTGAACATCTCACTTTTTTATTTTATTTTATTTTATTATTATTTTTTTTTTTATCAGAAAAGTATATAGAAAAAATTATATTGATGTTAAGTGACAAAATTGTAGCTTTTATTTTCTTTGAGTTCAACATGTAAGGATGAGATTATGCACAAACTAATTGAACTTGAAAATTTAAAAATAGAAATGAGATATTAAAGTTGGAGAAGATATATTATGAAAATATCTGAAATTGATAAAATCAAAGTTTTCACATAGGAATTTTTGATGAGTGGTAGGTGTCTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGAGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGGTTATTTAATTTCTTCTCTCCCACTTTTAATGACTTCCATTTTTCTTGGTTAGTGTTTGTTTCTGTTGTCTTATATGTGTTTTGTTGTTATCCTTCTTTCAAACTCTGCAGTTTGTAAGCGAACTAGTCAGAGTTGCAGCCCCAGGTGCCACCATAATTATAGTTACATGGTGCCATAGAGACCTTGGGCCTTCTGAAGACTCCTTGCAGCCATGGGAGGATAAGCTTCTTCAGAAGATTTGTGATGGCTTCTATCTCCCTGCTTGGTGTTCCACTGCTGATTATGTCAAATTACTCGAATCCCACAATCTCCAGGTTCTATATTCTTTATACTTTGAGCATCCTATCTATTTATTGATGGTCTTTCCCATAAATGATTTAAAAGGTGTTTAGAGCGTGGAGTTGAATTGTGATGCCAGAAGTTAATATGTTGGAGTTAAGAAGTTTGTGTATTCAATGTAACATAAGTTCATCAATAAATATGCAAGATAGAGTTATTTAACACTAATTATATTAATTAGAATTCCTCGGGTAGACTTCAATAATAAACACTATTGAAGCTAAGTTATTTAACATTAAGTATTGAAGTTGGTGGACCAAACACTACCTAAGAGAGTAAGTTGTTGAAATATATAAGGAATGTTTGGGATACGAGTGAAATTGTTTAGTCATATTTACAGTGTCAAGAGTTAATTCGATCATTGAATAGATATTTTTTAGTTATGAGACCCACGAGCTCCTTGGATCAAATAAGTAGTGAGCTCCTCCCAACTTTTACTCTTTGAAAAATGTAATTTTGGTGTATCTTATTAGTAAATATTGTAAAATTAAATAATTGTATCCTGAGATTAGTCTAGATATGGACTCACAAAACTATAAATTTTCAATGGTCAGGATCAGAACTATAACATAACGAATACAGAGAACCCATGTGGGAATCAGAGTTAATTTTTTGGGATGTGCGGAACATCTTCATAAAGTTGAATATTCTTTTATTTATTTGAATAAACTAAAAGTTATTATATTATTATTATTATTTTTTTTTACTATTCGAAAATGTGATGTAAAATGGGAGTGTGTGGTGTTGATAGGATATAAAGACAGCAGACTGGTCTCAGAACGTTGCTCCATTTTGGCCTGCGGTTATCCGATCTGCGTTGACTTGGAAAGGCTTCACATCACTCCTACGAAGTGGTAAATATTCCTGACACTAAAAATTAAAAATTAAAAAAAAAAGTCAATTTTAATCGATTAAAGGTAAATAAACAAATAAATAAAATCAATTTTTGTTAAAACGTTTCCAATATTCCTTTCTCAAACAACACTTTTTCTCAAAACACATTTTTCCAGATATCATTTTTGTCCTATATTTTAGATTTTATTGTTATACTTCCAATAGTTTAAAAGATAATTATTTTAAATAAAAAATAGTTAAAAATATTTTTAAGTGTACTAAAATGTCGTAATCTATCAATGATAGAAATTGATAAACATTAATAGTAGCTATCATATTTTGTTATATTTATAAATATATTTGGTTCATTTTGCTATATTTAAAAACAACTTAGTTTAAATTTAATTTTTGTATTTTCATTAAACCTTAAATTAATTATTCGAATTTAATTTCTATTGAAATCGGTTAAATAGTGATGATAACTTTAATGCAATAAAAAAAATACTATATGAATATCTATTTTTAATTCATAAATAGAAAGTTAATAGAATTTCTTTTTCATTTTTGAAAAAAACCCAATAATAATAGACTAGTATTAGGGTCTAAATTTAATATTTATGTTTAGGGGATGTACTTCACTGTTTAGATTTTATTACTAAGAAGATGAACATTTTGATTACATTGTTATTTTTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTATCTTTTAATTTTCAAAGTTTTAAAATTTTGCTAGGATTTTGAAAACATTTGTAAAGAGTAGATAAGAACAAAAGAAAAAAATTTAAAAGGAAAAATATTATTTATGATAAAATACCATTTTGTCACTTTACTTTGGACTTTGTTTATTAGTCATTATATTTTTAAATATCCAATTTTAATGGTTGTAATTTCAATGAATCATAAATCTAGTCATTTGATTCTTAAATAATAATAATAATAATAATTTTTGCATGATAGAAATATAATATGTATATGTCTTCAAGATTTATGGTAAAATGCTCTATATAAAAATTAGACATTTAAGATTTATCAAAAGTACATCGACTAAAATTAAACAAAGTTTGTTAAATAAATTTCAAAGTACAAATACTAAATAACATTAACCTAATACTTTATAAATTTAAAGTTTAAAAGTTTAAAAATAAAAAATAAAATAGTTACCTAACAAGTAAGTCTAAATTATTTGTTTTGAAATTATTGTTTTTGTCATTAAAAACATTTTATGAGCACTTGAAAAGCATGTTTATTTATTTGGTTGGGTTATATTATTACAATCAGGATGGAAAACAATAAAAGGAGCACTAGTAATGCCATTGATGATTGAAGGATTCAATAAAGATCTCATCAAATTTGCCATTATTACTTGCCGTAAGCCACATATGAAGCTTCATACAGCTAAATACCAAAAAGGAGTATTGTAAAACCTCACAACCAAATAATTCCCTCTTAGTTATTGTTCTTCCTATCTTTTCTTTTATTATTATTATTTTTTCTTTTTCTGTTACACGTTTCTCTTCAATACCTTATTAGTTTATGAGGGAAGAAAAATCCATGTTATTAGTTTATTTGTAATTTTTCTTTATTATTATGAGGTAGTTTTTTTTATTAGAAATTTTTTATTTTTCAAAGTTGTTTTTCTTAATTTACATTTATTGGTGTTAGCTAGTGTAACATTCACATTGTTATTTGTTAG

mRNA sequence

TACTCTTTCCAAAGAAAAAAGAAAAAGGAAATAACGTCTTCTCCTTTCTGTATCTTCTCTCTCAGCATCTCTGTGTTGCTTTACGTTCCCAACACATTGGCATCGGCCATTGCTCCCTTCTATATATTATTACCAACAATGAGCTTCACCGCTGCAACTCCAATCCATACGTCCTCGTATGCTCCTTCCTCTCTTCATTCCAATCGCTTCGACCCCACAATCCAATCTCCCATCCCTCTCCGCCGTTCCTTTTCTTCTTTCACCCCCCGCCGCACCTCTCCTCTGCTTGCTGCCGCTGCGACGACGACCAAGACGGTCCCCGACCAGATGGACTCCGATCGCCTTCAGAAAGGAATCGCCGAGTTCTACGATGAATCTTCGGGGCTTTGGGAGAGTGTTTGGGGCGAACATATGCACCATGGATTTTACGATCCTGATTCTTCCGTTTCCCTCTCCGATCACCGCGCTGCGCAAATCCGGATGATCGACGAGACGCTCCGGTTCGCAGGCGTGGAGGCGGCGATGGCGGCGGTGGACGTTGGGTGTGGGATTGGGGGAAGTTCTAGGTATTTGGCGAGGAAGTTTGGGGCGAAATGTAGAGGTATAACTTTGAGCCCTGTTCAAGCTAAAAGGGCTCAAGCGATTGCTGCGGCTGAAGGATTAAGTGATAAGGTGTCTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGAGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGTTTGTAAGCGAACTAGTCAGAGTTGCAGCCCCAGGTGCCACCATAATTATAGTTACATGGTGCCATAGAGACCTTGGGCCTTCTGAAGACTCCTTGCAGCCATGGGAGGATAAGCTTCTTCAGAAGATTTGTGATGGCTTCTATCTCCCTGCTTGGTGTTCCACTGCTGATTATGTCAAATTACTCGAATCCCACAATCTCCAGGATATAAAGACAGCAGACTGGTCTCAGAACGTTGCTCCATTTTGGCCTGCGGTTATCCGATCTGCGTTGACTTGGAAAGGCTTCACATCACTCCTACGAAGTGGATGGAAAACAATAAAAGGAGCACTAGTAATGCCATTGATGATTGAAGGATTCAATAAAGATCTCATCAAATTTGCCATTATTACTTGCCGTAAGCCACATATGAAGCTTCATACAGCTAAATACCAAAAAGGAGTATTGTAAAACCTCACAACCAAATAATTCCCTCTTAGTTATTGTTCTTCCTATCTTTTCTTTTATTATTATTATTTTTTCTTTTTCTGTTACACGTTTCTCTTCAATACCTTATTAGTTTATGAGGGAAGAAAAATCCATGTTATTAGTTTATTTGTAATTTTTCTTTATTATTATGAGGTAGTTTTTTTTATTAGAAATTTTTTATTTTTCAAAGTTGTTTTTCTTAATTTACATTTATTGGTGTTAGCTAGTGTAACATTCACATTGTTATTTGTTAG

Coding sequence (CDS)

ATGAGCTTCACCGCTGCAACTCCAATCCATACGTCCTCGTATGCTCCTTCCTCTCTTCATTCCAATCGCTTCGACCCCACAATCCAATCTCCCATCCCTCTCCGCCGTTCCTTTTCTTCTTTCACCCCCCGCCGCACCTCTCCTCTGCTTGCTGCCGCTGCGACGACGACCAAGACGGTCCCCGACCAGATGGACTCCGATCGCCTTCAGAAAGGAATCGCCGAGTTCTACGATGAATCTTCGGGGCTTTGGGAGAGTGTTTGGGGCGAACATATGCACCATGGATTTTACGATCCTGATTCTTCCGTTTCCCTCTCCGATCACCGCGCTGCGCAAATCCGGATGATCGACGAGACGCTCCGGTTCGCAGGCGTGGAGGCGGCGATGGCGGCGGTGGACGTTGGGTGTGGGATTGGGGGAAGTTCTAGGTATTTGGCGAGGAAGTTTGGGGCGAAATGTAGAGGTATAACTTTGAGCCCTGTTCAAGCTAAAAGGGCTCAAGCGATTGCTGCGGCTGAAGGATTAAGTGATAAGGTGTCTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGAGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGTTTGTAAGCGAACTAGTCAGAGTTGCAGCCCCAGGTGCCACCATAATTATAGTTACATGGTGCCATAGAGACCTTGGGCCTTCTGAAGACTCCTTGCAGCCATGGGAGGATAAGCTTCTTCAGAAGATTTGTGATGGCTTCTATCTCCCTGCTTGGTGTTCCACTGCTGATTATGTCAAATTACTCGAATCCCACAATCTCCAGGATATAAAGACAGCAGACTGGTCTCAGAACGTTGCTCCATTTTGGCCTGCGGTTATCCGATCTGCGTTGACTTGGAAAGGCTTCACATCACTCCTACGAAGTGGATGGAAAACAATAAAAGGAGCACTAGTAATGCCATTGATGATTGAAGGATTCAATAAAGATCTCATCAAATTTGCCATTATTACTTGCCGTAAGCCACATATGAAGCTTCATACAGCTAAATACCAAAAAGGAGTATTGTAA

Protein sequence

MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTSPLLAAAATTTKTVPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDETLRFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVSFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVIRSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKPHMKLHTAKYQKGVL
Homology
BLAST of CcUC02G025100 vs. NCBI nr
Match: XP_038900464.1 (tocopherol O-methyltransferase, chloroplastic [Benincasa hispida])

HSP 1 Score: 650.2 bits (1676), Expect = 1.0e-182
Identity = 322/348 (92.53%), Postives = 330/348 (94.83%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTSPLLAAAATTTKTV 60
           MSFT A PIHT SY+PSS HSN F PTI SPIPLRRSFSSFT RRTSPLLAAAA TT + 
Sbjct: 1   MSFTTAPPIHTLSYSPSSFHSNPFKPTILSPIPLRRSFSSFTHRRTSPLLAAAAATTAS- 60

Query: 61  PDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDETL 120
            DQMD+DRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSL+DHRAAQIRMI+ETL
Sbjct: 61  -DQMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLADHRAAQIRMIEETL 120

Query: 121 RFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVS 180
           RFA VE A AAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV 
Sbjct: 121 RFASVEKATAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPG TIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGGTIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVI 300
           SE SLQPWE+KLLQKICDGFYLPAWCSTADYVKLL+SHNLQDIKTADWS+NVAPFWPAVI
Sbjct: 241 SEKSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKTADWSENVAPFWPAVI 300

Query: 301 RSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           RSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP
Sbjct: 301 RSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 346

BLAST of CcUC02G025100 vs. NCBI nr
Match: XP_008462787.1 (PREDICTED: tocopherol O-methyltransferase, chloroplastic [Cucumis melo] >TYK00989.1 tocopherol O-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 631.7 bits (1628), Expect = 3.9e-177
Identity = 313/348 (89.94%), Postives = 323/348 (92.82%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTSPLLAAAATTTKTV 60
           M+FTAATPIHTS Y PSSLH NR +PTI +PI LR SFSSFTPRR+SP LA AATT    
Sbjct: 1   MNFTAATPIHTSFYPPSSLHFNRLNPTILNPISLRPSFSSFTPRRSSPFLAFAATTAS-- 60

Query: 61  PDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDETL 120
            D MDS RLQKGIAEFYD+SSGLWE+VWGEHMHHGFYDPDSSVSLSDHRAAQ+RMI+ETL
Sbjct: 61  -DHMDSHRLQKGIAEFYDQSSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQVRMIEETL 120

Query: 121 RFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVS 180
           RFAGVE A A VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV 
Sbjct: 121 RFAGVEEATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVY 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SHNLQDIK ADWS NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSGNVAPFWPAVI 300

Query: 301 RSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           RSA TWKGFTSLLR+GWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 345

BLAST of CcUC02G025100 vs. NCBI nr
Match: KAG7011011.1 (Tocopherol O-methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 619.8 bits (1597), Expect = 1.5e-173
Identity = 311/349 (89.11%), Postives = 322/349 (92.26%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTS-PLLAAAATTTKT 60
           MSFT ATPI T SY+ SSLHSNRF+ TI SPIPLRRSFSSF  RR + PL+AAAATTT  
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  VPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDET 120
             D MD D L+KGIAEFYDESSGLWE+VWGEHMHHGFYDPDSSVSLSDHRAAQIRMI+ET
Sbjct: 61  EID-MDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET 120

Query: 121 LRFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV 180
           LRFAGVEAA A VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAA  GLSDKV
Sbjct: 121 LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV 180

Query: 181 SFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240
            FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG
Sbjct: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240

Query: 241 PSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAV 300
           PSEDSLQPWE KLLQKICDGFYLPAWCSTADYVKLLESH LQD++TADWS+NVAPFWPAV
Sbjct: 241 PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAV 300

Query: 301 IRSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           IRSA TW GF+SLLRSGWKTIKGALVMPLMIEGF KDLIKFAIITCRKP
Sbjct: 301 IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKP 348

BLAST of CcUC02G025100 vs. NCBI nr
Match: XP_023513404.1 (tocopherol O-methyltransferase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619.0 bits (1595), Expect = 2.6e-173
Identity = 311/349 (89.11%), Postives = 321/349 (91.98%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSS-FTPRRTSPLLAAAATTTKT 60
           MSFT ATPI T SY+ SSLHSNRF+ TI SPIPLRRSFSS F  RR  PL+AAAATTT  
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFFRRRRNCPLIAAAATTTDV 60

Query: 61  VPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDET 120
             D MD D L+KGIAEFYDESSGLWE+VWGEHMHHGFYDPDSSVSLSDHRAAQIRMI+ET
Sbjct: 61  EID-MDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET 120

Query: 121 LRFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV 180
           LRFAGVEAA A VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAA  GLSDKV
Sbjct: 121 LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV 180

Query: 181 SFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240
            FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG
Sbjct: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240

Query: 241 PSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAV 300
           PSEDSLQPWE KLLQKICDGFYLPAWCSTADYVKLLESH LQD++TADWS+NVAPFWPAV
Sbjct: 241 PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAV 300

Query: 301 IRSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           IRSA TW GF+SLLRSGWKTIKGALVMPLMIEGF KDLIKFAIITCRKP
Sbjct: 301 IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKP 348

BLAST of CcUC02G025100 vs. NCBI nr
Match: KAG6571208.1 (Tocopherol O-methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 617.8 bits (1592), Expect = 5.8e-173
Identity = 310/349 (88.83%), Postives = 321/349 (91.98%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTS-PLLAAAATTTKT 60
           MSFT ATPI T SY+ SSLH NRF+ TI SPIPLRRSFSSF  RR + PL+AAAATTT  
Sbjct: 1   MSFTTATPILTPSYSLSSLHCNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  VPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDET 120
             D MD D L+KGIAEFYDESSGLWE+VWGEHMHHGFYDPDSSVSLSDHRAAQIRMI+ET
Sbjct: 61  EID-MDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET 120

Query: 121 LRFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV 180
           LRFAGVEAA A VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAA  GLSDKV
Sbjct: 121 LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV 180

Query: 181 SFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240
            FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG
Sbjct: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240

Query: 241 PSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAV 300
           PSEDSLQPWE KLLQKICDGFYLPAWCSTADYVKLLESH LQD++TADWS+NVAPFWPAV
Sbjct: 241 PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAV 300

Query: 301 IRSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           IRSA TW GF+SLLRSGWKTIKGALVMPLMIEGF KDLIKFAIITCRKP
Sbjct: 301 IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKP 348

BLAST of CcUC02G025100 vs. ExPASy Swiss-Prot
Match: Q9ZSK1 (Tocopherol O-methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VTE4 PE=1 SV=2)

HSP 1 Score: 451.4 bits (1160), Expect = 9.4e-126
Identity = 233/358 (65.08%), Postives = 271/358 (75.70%), Query Frame = 0

Query: 1   MSFTAATPIHTSS--YAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTSPLLAAAATTTK 60
           M  T A P   +S  Y  +S   ++     +S  P   S  S T  R +  +AAAAT+T 
Sbjct: 1   MKATLAAPSSLTSLPYRTNSSFGSKSSLLFRS--PSSSSSVSMTTTRGNVAVAAAATST- 60

Query: 61  TVPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSD--HRAAQIRMI 120
                   + L+KGIAEFY+E+SGLWE +WG+HMHHGFYDPDSSV LSD  H+ AQIRMI
Sbjct: 61  --------EALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMI 120

Query: 121 DETLRFAGV------EAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIA 180
           +E+LRFAGV      +     VDVGCGIGGSSRYLA KFGA+C GITLSPVQAKRA  +A
Sbjct: 121 EESLRFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLA 180

Query: 181 AAEGLSDKVSFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIII 240
           AA+ L+ K SF+VADAL+QPF DG+FDLVWSMESGEHMPDK+KFV ELVRVAAPG  III
Sbjct: 181 AAQSLAHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIII 240

Query: 241 VTWCHRDLGPSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQ 300
           VTWCHR+L   E++LQPWE  +L KIC  FYLPAWCST DYV LL+SH+LQDIK ADWS+
Sbjct: 241 VTWCHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIKCADWSE 300

Query: 301 NVAPFWPAVIRSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           NVAPFWPAVIR+ALTWKG  SLLRSG K+IKGAL MPLMIEG+ K +IKF IITC+KP
Sbjct: 301 NVAPFWPAVIRTALTWKGLVSLLRSGMKSIKGALTMPLMIEGYKKGVIKFGIITCQKP 347

BLAST of CcUC02G025100 vs. ExPASy Swiss-Prot
Match: Q6ZIK0 (Probable tocopherol O-methyltransferase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=VTE4 PE=2 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.1e-123
Identity = 214/310 (69.03%), Postives = 253/310 (81.61%), Query Frame = 0

Query: 44  RRTSPLLAAAATTTKTVPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSV 103
           R  +P L++++T     P       L++GIA  YDESSG+WES+WGEHMHHGFYD   + 
Sbjct: 55  RAMAPTLSSSSTAAAAPPG------LKEGIAGLYDESSGVWESIWGEHMHHGFYDAGEAA 114

Query: 104 SLSDHRAAQIRMIDETLRFAGV-----EAAMAAVDVGCGIGGSSRYLARKFGAKCRGITL 163
           S+SDHR AQIRMI+E+L FA V     +   + VDVGCGIGGSSRYLA K+GA+C GITL
Sbjct: 115 SMSDHRRAQIRMIEESLAFAAVPDDAEKKPKSVVDVGCGIGGSSRYLANKYGAQCYGITL 174

Query: 164 SPVQAKRAQAIAAAEGLSDKVSFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSEL 223
           SPVQA+R  A+AA +GLSDKVSF+V DAL QPF DG+FDLVWSMESGEHMPDK +FVSEL
Sbjct: 175 SPVQAERGNALAAEQGLSDKVSFQVGDALEQPFPDGQFDLVWSMESGEHMPDKRQFVSEL 234

Query: 224 VRVAAPGATIIIVTWCHRDLGPSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESH 283
            RVAAPGA IIIVTWCHR+L PSE+SL+P E  LL++ICD +YLP WCS +DYVK+ ES 
Sbjct: 235 ARVAAPGARIIIVTWCHRNLEPSEESLKPDELNLLKRICDAYYLPDWCSPSDYVKIAESL 294

Query: 284 NLQDIKTADWSQNVAPFWPAVIRSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLI 343
           +L+DI+TADWS+NVAPFWPAVI+SALTWKG TSLLRSGWKTI+GA+VMPLMIEG+ K LI
Sbjct: 295 SLEDIRTADWSENVAPFWPAVIKSALTWKGLTSLLRSGWKTIRGAMVMPLMIEGYKKGLI 354

Query: 344 KFAIITCRKP 349
           KF IITCRKP
Sbjct: 355 KFTIITCRKP 358

BLAST of CcUC02G025100 vs. ExPASy Swiss-Prot
Match: W5U2K2 (3-hydroxy-16-methoxy-2,3-dihydrotabersonine N-methyltransferase OS=Catharanthus roseus OX=4058 GN=NMT PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.9e-71
Identity = 132/284 (46.48%), Postives = 197/284 (69.37%), Query Frame = 0

Query: 70  QKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDETLRFAGV---- 129
           Q+ +AEFYD+ +G W+  +G H+H G+Y+P ++ +++  + A IRMIDE LRFAGV    
Sbjct: 5   QEKVAEFYDKVTGAWDLFYGVHLHDGYYEPGTTATMAISQDAVIRMIDELLRFAGVSEDP 64

Query: 130 -EAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVSFEVA 189
            +   + +DVG G+GG+  Y+A+K+  +C GIT+SP Q K AQ  AA EG+ +KVSF+  
Sbjct: 65  AKKPRSMLDVGSGLGGTCVYVAKKYDIQCTGITISPNQVKYAQDYAATEGVENKVSFDCG 124

Query: 190 DALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSEDS 249
           DAL+ P+SDG+FD+V+++   EH+ DK KF+ E+VRVAAPGA III +  H +L P E S
Sbjct: 125 DALDMPYSDGKFDVVFTINCIEHVHDKEKFIREMVRVAAPGAAIIIASQAHPNLSPGE-S 184

Query: 250 LQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVIRSAL 309
           L+P + K+LQKICDG    + CS+ DYV+ L    +++IK ADW+QN+ P +P +++ A 
Sbjct: 185 LKPRDKKILQKICDGAGAVSLCSSDDYVRWLTPLPVKEIKAADWTQNITPLYPLLMKEAF 244

Query: 310 TWKGFTSL-LRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRK 348
           TWKGFTS+ L+ GW+ I     + L+ +  N  ++KFA++T RK
Sbjct: 245 TWKGFTSIVLKGGWRAINLINAVRLVAKAANDGILKFAVVTGRK 287

BLAST of CcUC02G025100 vs. ExPASy Swiss-Prot
Match: P74388 (2-methyl-6-phytyl-1,4-hydroquinone methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll0418 PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 5.8e-35
Identity = 95/287 (33.10%), Postives = 146/287 (50.87%), Query Frame = 0

Query: 73  IAEFYDE--SSGLWESVWGEHMHHGFY-DPDSSVSLSDHRAAQIRMIDETLRFAGVEA-- 132
           +A  YD+    G+ E  WG+H+H G Y DP  +    D   ++I  +    ++ G++   
Sbjct: 36  VANAYDQWTEDGILEYYWGDHIHLGHYGDPPVA---KDFIQSKIDFVHAMAQWGGLDTLP 95

Query: 133 -AMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVSFEVADA 192
                +DVGCGIGGSSR LA+ +G    GIT+SP Q KRA  +   +  +    F V DA
Sbjct: 96  PGTTVLDVGCGIGGSSRILAKDYGFNVTGITISPQQVKRATELTPPDVTA---KFAVDDA 155

Query: 193 LNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSEDSLQ 252
           +   F DG FD+VWS+E+G HMPDK+ F  EL+RV  PG  +++  W  RD    +  L 
Sbjct: 156 MALSFPDGSFDVVWSVEAGPHMPDKAVFAKELLRVVKPGGILVVADWNQRD--DRQVPLN 215

Query: 253 PWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQD--IKTADWSQNVAPFWPAVIRSAL 312
            WE  +++++ D +  PA+ S   + + LE+  L +  + TADW+    P W   I    
Sbjct: 216 FWEKPVMRQLLDQWSHPAFASIEGFAENLEATGLVEGQVTTADWTVPTLPAWLDTI---- 275

Query: 313 TWKGFTSLLRSGW---------KTIKGALVMPLMIEGFNKDLIKFAI 343
            W+G   +   GW         K+++    + LM   F   L +F +
Sbjct: 276 -WQGI--IRPQGWLQYGIRGFIKSVREVPTILLMRLAFGVGLCRFGM 307

BLAST of CcUC02G025100 vs. ExPASy Swiss-Prot
Match: Q8KZ94 (Demethylrebeccamycin-D-glucose O-methyltransferase OS=Lentzea aerocolonigenes OX=68170 GN=rebM PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.1e-24
Identity = 60/159 (37.74%), Postives = 94/159 (59.12%), Query Frame = 0

Query: 73  IAEFYDESSGLWESVWGEHMHHGFY-DPDSSVSLSDHRAAQIRMIDETLRFAGVEAAMAA 132
           + + YD+ +  +  +WGE++H G++ D  + VS+ D   A  R+ DE +    V +    
Sbjct: 19  VRQMYDDFTDPFARIWGENLHFGYWEDAGADVSVDD---ATDRLTDEMIALLDVRSGDRV 78

Query: 133 VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVSFEVADALNQPF 192
           +DVGCGIG  +  LA     +  GI++S  Q  +A A A A GL+++V+F  ADA++ PF
Sbjct: 79  LDVGCGIGKPAVRLATARDVRVTGISISRPQVNQANARATAAGLANRVTFSYADAMDLPF 138

Query: 193 SDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIII 231
            D  FD VW++ES  HMPD+ + + E+ RV  PG T+ I
Sbjct: 139 EDASFDAVWALESLHHMPDRGRALREMARVLRPGGTVAI 174

BLAST of CcUC02G025100 vs. ExPASy TrEMBL
Match: A0A5D3BSC2 (Tocopherol O-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001840 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.9e-177
Identity = 313/348 (89.94%), Postives = 323/348 (92.82%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTSPLLAAAATTTKTV 60
           M+FTAATPIHTS Y PSSLH NR +PTI +PI LR SFSSFTPRR+SP LA AATT    
Sbjct: 1   MNFTAATPIHTSFYPPSSLHFNRLNPTILNPISLRPSFSSFTPRRSSPFLAFAATTAS-- 60

Query: 61  PDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDETL 120
            D MDS RLQKGIAEFYD+SSGLWE+VWGEHMHHGFYDPDSSVSLSDHRAAQ+RMI+ETL
Sbjct: 61  -DHMDSHRLQKGIAEFYDQSSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQVRMIEETL 120

Query: 121 RFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVS 180
           RFAGVE A A VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV 
Sbjct: 121 RFAGVEEATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVY 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SHNLQDIK ADWS NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSGNVAPFWPAVI 300

Query: 301 RSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           RSA TWKGFTSLLR+GWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 345

BLAST of CcUC02G025100 vs. ExPASy TrEMBL
Match: A0A1S3CHR0 (tocopherol O-methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501078 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.9e-177
Identity = 313/348 (89.94%), Postives = 323/348 (92.82%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTSPLLAAAATTTKTV 60
           M+FTAATPIHTS Y PSSLH NR +PTI +PI LR SFSSFTPRR+SP LA AATT    
Sbjct: 1   MNFTAATPIHTSFYPPSSLHFNRLNPTILNPISLRPSFSSFTPRRSSPFLAFAATTAS-- 60

Query: 61  PDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDETL 120
            D MDS RLQKGIAEFYD+SSGLWE+VWGEHMHHGFYDPDSSVSLSDHRAAQ+RMI+ETL
Sbjct: 61  -DHMDSHRLQKGIAEFYDQSSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQVRMIEETL 120

Query: 121 RFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVS 180
           RFAGVE A A VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV 
Sbjct: 121 RFAGVEEATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVY 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SHNLQDIK ADWS NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSGNVAPFWPAVI 300

Query: 301 RSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           RSA TWKGFTSLLR+GWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 345

BLAST of CcUC02G025100 vs. ExPASy TrEMBL
Match: A0A6J1FYT6 (tocopherol O-methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449074 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 2.8e-173
Identity = 310/349 (88.83%), Postives = 321/349 (91.98%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTS-PLLAAAATTTKT 60
           MSFT ATPI T SY+ SSLHSNRF+  I SPIPLRRSFSSF  RR + PL+AAAATTT  
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNAAILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  VPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDET 120
             D MD D L+KGIAEFYDESSGLWE+VWGEHMHHGFYDPDSSVSLSDHRAAQIRMI+ET
Sbjct: 61  EID-MDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET 120

Query: 121 LRFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV 180
           LRFAGVEAA A VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAA  GLSDKV
Sbjct: 121 LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV 180

Query: 181 SFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240
            FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG
Sbjct: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240

Query: 241 PSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAV 300
           PSEDSLQPWE KLLQKICDGFYLPAWCSTADYVKLLESH LQD++TADWS+NVAPFWPAV
Sbjct: 241 PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAV 300

Query: 301 IRSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           IRSA TW GF+SLLRSGWKTIKGALVMPLMIEGF KDLIKFAIITCRKP
Sbjct: 301 IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKP 348

BLAST of CcUC02G025100 vs. ExPASy TrEMBL
Match: A0A6J1JGD5 (tocopherol O-methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484262 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.2e-171
Identity = 310/350 (88.57%), Postives = 321/350 (91.71%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSF-SSFTP-RRTSPLLAAAATTTK 60
           MSFTAATPI T SY+ SSLHSNRF  TI SPIPLRRSF SSFT  RR   L+A AATTT 
Sbjct: 1   MSFTAATPILTPSYSLSSLHSNRFKATILSPIPLRRSFSSSFTRCRRNCSLIATAATTTD 60

Query: 61  TVPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDE 120
              D MD DRL++GIAEFYDESSGLWE+VWGEHMHHGFYDPDSSVSLSDHRAAQIRMI+E
Sbjct: 61  VEID-MDVDRLKEGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEE 120

Query: 121 TLRFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDK 180
           TLRFAGVEAA A VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAA  GLSDK
Sbjct: 121 TLRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDK 180

Query: 181 VSFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDL 240
           V FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDL
Sbjct: 181 VCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDL 240

Query: 241 GPSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPA 300
           GPSEDSLQPWE KLLQKICDGFYLPAWCSTADYVKL+ESH LQD++TADWS+NVAPFWPA
Sbjct: 241 GPSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLVESHGLQDVRTADWSRNVAPFWPA 300

Query: 301 VIRSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           VIRSA TW GF+SLLRSGWKTIKGALVMPLMIEGF KDLIKFAIITCRKP
Sbjct: 301 VIRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKP 349

BLAST of CcUC02G025100 vs. ExPASy TrEMBL
Match: A0A0A0LTM1 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G432780 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.1e-168
Identity = 299/348 (85.92%), Postives = 313/348 (89.94%), Query Frame = 0

Query: 1   MSFTAATPIHTSSYAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTSPLLAAAATTTKTV 60
           M+ TAATPIHTSSY PSSLHSN F     + I  R SFSSFTPRR SPLLA+++  T   
Sbjct: 1   MNLTAATPIHTSSYPPSSLHSNPF-----NSISPRPSFSSFTPRRFSPLLASSSAATTPP 60

Query: 61  PDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDETL 120
              MDS RL KGIAEFYD+SSGLWE+VWG+HMHHGFYDP SSVSLSDHRAAQIRMI+ETL
Sbjct: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVS 180
           RFAGVEAA A VDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+AAEGLSDKV 
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SHNLQDIK ADWS+NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300

Query: 301 RSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           RSA TWKGFTSLLR+GWKTI+GALVMPLMIEGFNKDLIKFAIITCRKP
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKP 343

BLAST of CcUC02G025100 vs. TAIR 10
Match: AT1G64970.1 (gamma-tocopherol methyltransferase )

HSP 1 Score: 451.4 bits (1160), Expect = 6.6e-127
Identity = 233/358 (65.08%), Postives = 271/358 (75.70%), Query Frame = 0

Query: 1   MSFTAATPIHTSS--YAPSSLHSNRFDPTIQSPIPLRRSFSSFTPRRTSPLLAAAATTTK 60
           M  T A P   +S  Y  +S   ++     +S  P   S  S T  R +  +AAAAT+T 
Sbjct: 1   MKATLAAPSSLTSLPYRTNSSFGSKSSLLFRS--PSSSSSVSMTTTRGNVAVAAAATST- 60

Query: 61  TVPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSD--HRAAQIRMI 120
                   + L+KGIAEFY+E+SGLWE +WG+HMHHGFYDPDSSV LSD  H+ AQIRMI
Sbjct: 61  --------EALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMI 120

Query: 121 DETLRFAGV------EAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIA 180
           +E+LRFAGV      +     VDVGCGIGGSSRYLA KFGA+C GITLSPVQAKRA  +A
Sbjct: 121 EESLRFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLA 180

Query: 181 AAEGLSDKVSFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIII 240
           AA+ L+ K SF+VADAL+QPF DG+FDLVWSMESGEHMPDK+KFV ELVRVAAPG  III
Sbjct: 181 AAQSLAHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIII 240

Query: 241 VTWCHRDLGPSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQ 300
           VTWCHR+L   E++LQPWE  +L KIC  FYLPAWCST DYV LL+SH+LQDIK ADWS+
Sbjct: 241 VTWCHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIKCADWSE 300

Query: 301 NVAPFWPAVIRSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKP 349
           NVAPFWPAVIR+ALTWKG  SLLRSG K+IKGAL MPLMIEG+ K +IKF IITC+KP
Sbjct: 301 NVAPFWPAVIRTALTWKGLVSLLRSGMKSIKGALTMPLMIEGYKKGVIKFGIITCQKP 347

BLAST of CcUC02G025100 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 80.9 bits (198), Expect = 2.4e-15
Identity = 63/242 (26.03%), Postives = 104/242 (42.98%), Query Frame = 0

Query: 56  TTKTVPDQMDSDRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRM 115
           T + VPD +D+         FY+  + ++E  WG+  H   + P  S+    H+ A    
Sbjct: 66  TAEKVPDFVDT---------FYNLVTDIYEWGWGQSFH---FSP--SIPGKSHKDATRLH 125

Query: 116 IDETLRFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGL 175
            +  +    V+     +DVGCG+GG  R +A    A   GIT++  Q  RA+      GL
Sbjct: 126 EEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGL 185

Query: 176 SDKVSFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCH 235
                    + L  PF D  FD  +S+E+  H P   +  +E+ RV  PG+  +   W  
Sbjct: 186 DALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVT 245

Query: 236 RDLGPSEDSLQPWEDKLLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVA-P 295
            +   +ED       +++Q I  G  LP   +  D  +  +    + +K  D +   A P
Sbjct: 246 TEKFKAEDDEHV---EVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEP 290

Query: 296 FW 297
           +W
Sbjct: 306 WW 290

BLAST of CcUC02G025100 vs. TAIR 10
Match: AT1G76090.1 (sterol methyltransferase 3 )

HSP 1 Score: 79.3 bits (194), Expect = 6.9e-15
Identity = 62/239 (25.94%), Postives = 108/239 (45.19%), Query Frame = 0

Query: 61  PDQMDS-DRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDET 120
           P +++S +++   +  FY+  + ++E  WG+  H   + P      SD  A +I   +  
Sbjct: 61  PKEIESAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPHVPGK----SDKDATRIHE-EMA 120

Query: 121 LRFAGVEAAMAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKV 180
           +    V+     +D GCG+GG  R +A    A+  GIT++  Q +RA+      GL    
Sbjct: 121 VDLIKVKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGLDSLC 180

Query: 181 SFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240
           +    + L  PF +  FD  +S+E+  H P   +  SE+ RV  PG+  +   W   +  
Sbjct: 181 NVVCGNFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKY 240

Query: 241 PSEDSLQPWEDK-LLQKICDGFYLPAWCSTADYVKLLESHNLQDIKTADWSQNVA-PFW 297
             +D     E K ++Q I  G  LP   S AD     +    + +K  D ++  + P+W
Sbjct: 241 RDDDE----EHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWW 290

BLAST of CcUC02G025100 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 76.3 bits (186), Expect = 5.8e-14
Identity = 46/165 (27.88%), Postives = 72/165 (43.64%), Query Frame = 0

Query: 73  IAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDETLRFAGVEAAMAAV 132
           + ++YD ++  +E  WGE  H        S+  S  R      +       G++     +
Sbjct: 45  VNKYYDLATSFYEYGWGESFHFAQRWKGESLRESIKRHEHFLALQ-----LGIQPGQKVL 104

Query: 133 DVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVSFEVADALNQPFS 192
           DVGCGIGG  R +AR   +   G+  +  Q  R + +    G+    +F  AD +  PF 
Sbjct: 105 DVGCGIGGPLREIARFSNSVVTGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFP 164

Query: 193 DGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRD 238
           +  FD V+++E+  H PD      E+ RV  PG       WC  D
Sbjct: 165 ENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTD 204

BLAST of CcUC02G025100 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 76.3 bits (186), Expect = 5.8e-14
Identity = 46/165 (27.88%), Postives = 72/165 (43.64%), Query Frame = 0

Query: 73  IAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIDETLRFAGVEAAMAAV 132
           + ++YD ++  +E  WGE  H        S+  S  R      +       G++     +
Sbjct: 45  VNKYYDLATSFYEYGWGESFHFAQRWKGESLRESIKRHEHFLALQ-----LGIQPGQKVL 104

Query: 133 DVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVSFEVADALNQPFS 192
           DVGCGIGG  R +AR   +   G+  +  Q  R + +    G+    +F  AD +  PF 
Sbjct: 105 DVGCGIGGPLREIARFSNSVVTGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFP 164

Query: 193 DGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRD 238
           +  FD V+++E+  H PD      E+ RV  PG       WC  D
Sbjct: 165 ENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTD 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900464.11.0e-18292.53tocopherol O-methyltransferase, chloroplastic [Benincasa hispida][more]
XP_008462787.13.9e-17789.94PREDICTED: tocopherol O-methyltransferase, chloroplastic [Cucumis melo] >TYK0098... [more]
KAG7011011.11.5e-17389.11Tocopherol O-methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. arg... [more]
XP_023513404.12.6e-17389.11tocopherol O-methyltransferase, chloroplastic [Cucurbita pepo subsp. pepo][more]
KAG6571208.15.8e-17388.83Tocopherol O-methyltransferase, chloroplastic, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
Q9ZSK19.4e-12665.08Tocopherol O-methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q6ZIK01.1e-12369.03Probable tocopherol O-methyltransferase, chloroplastic OS=Oryza sativa subsp. ja... [more]
W5U2K23.9e-7146.483-hydroxy-16-methoxy-2,3-dihydrotabersonine N-methyltransferase OS=Catharanthus ... [more]
P743885.8e-3533.102-methyl-6-phytyl-1,4-hydroquinone methyltransferase OS=Synechocystis sp. (strai... [more]
Q8KZ942.1e-2437.74Demethylrebeccamycin-D-glucose O-methyltransferase OS=Lentzea aerocolonigenes OX... [more]
Match NameE-valueIdentityDescription
A0A5D3BSC21.9e-17789.94Tocopherol O-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CHR01.9e-17789.94tocopherol O-methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A6J1FYT62.8e-17388.83tocopherol O-methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JGD51.2e-17188.57tocopherol O-methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A0A0LTM12.1e-16885.92Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G43... [more]
Match NameE-valueIdentityDescription
AT1G64970.16.6e-12765.08gamma-tocopherol methyltransferase [more]
AT1G20330.12.4e-1526.03sterol methyltransferase 2 [more]
AT1G76090.16.9e-1525.94sterol methyltransferase 3 [more]
AT5G13710.15.8e-1427.88sterol methyltransferase 1 [more]
AT5G13710.25.8e-1427.88sterol methyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 132..230
e-value: 1.9E-18
score: 66.9
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 78..324
e-value: 2.0E-57
score: 196.4
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 58..344
NoneNo IPR availablePANTHERPTHR43591:SF72CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASEcoord: 58..344
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 132..233
e-value: 1.43498E-10
score: 55.899
IPR025774SAM-dependent methyltransferase gTMT-typePROSITEPS51581SAM_GTMTcoord: 52..349
score: 143.824112
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 68..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC02G025100.1CcUC02G025100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0010189 vitamin E biosynthetic process
molecular_function GO:0050342 tocopherol O-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity