CcUC02G022300 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC02G022300
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionCytochrome P450 84A1-like
LocationCicolChr02: 4669345 .. 4672742 (-)
RNA-Seq ExpressionCcUC02G022300
SyntenyCcUC02G022300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTACTCATTATTTATTGGCATTCCATATAAGTGCGCTACATCCATACCTCCCTAACTGAAGTTCATAGAATCGAATGCTACATTTTAGAAGTAAGTACATAAGGCATAAGCAACCTCCTTTTAAAAATAAATTTACGACATTTTTTCAAGAAAGTTACACACTTGTTATTTATTGTAATAAATTGTCACAAACCTTTGAATTAAAAATATATAACAAGGCAGAGTTATCAGTTTACTAAATTAAGATATCATACTCCCTCCCATCAATTTCCCTTTCTCAAATTCATGCTCTCAAAAGACAAAACCAAAATCCTTCATCTCTATAAATTCAATATTCCTCTCTAAATCCATTCAAATTCAAACACTATCATACGTCAACTCAATGGAGTTTCTAAACCCTTCTTCTTTTCTCTTCTTCTCTTTGTCGCTTTTTTGCGAAATTTACATCGTAAATTTAGCTACCCGCCTGGCCCCGGTGGATTGCCCATTATCGGCAACATGCTGATGATGGGCCAACTCCCCCACCGTGGCCTCGCCCGTCTCGCCGCCATCTACGGTGGCCTCTTCCACCTTCGACTCGGCGTCCTCCACATGGTGGTTGTCTCCACGCCAGATATGGCACGAGAATTCCTCCAAGTTCAAGACGCAGCGTTCGCAAATAGGGCTGCAAACGTTGCGATCACATATCTCACCTACGATCGCGCCGATATGGCCTTCGCCAACTACGGCCCATTTTGGCGCCAAATGCGAAAGATTTGCGTCATCAAGCTCTTCAGCCGCCGACGCGCAGAGTCATGGGCGTCGGTGCGTGACGAAGTCGACGCCCTAGTCCGAATGGTTTCAAGTAAAATGGGGCAACCGGTGAATATCGGTGACCTAGTTTTCAGCCTGACACGAAACATAACCTACAAGGCCGCGTTTGGATCGAGGTCGCACGAAGGACAGGATGAGTTGGTTAAGATCTTGCAAGAATTCTCTAAACTGTTTGGAGCTTTTAATATTGCACATTTGTTGCCATGGTTGGGATGGATTCATGCTCGAGATTTCAATGAAAGAATGGCAAAAGCTAGAAGAGATCTTGCTGTGTTCATAAATACCATAATTGATGAACATTTACAAAAGAAAAGGAAGAAAAAGGGAAAAGATGAAGATGATGAAGAAGAAGATTCAGATATGGTTGATGAATTAATGGCATTCCTTGCTGATGAGTCAAGTAACAATGAGTTCGATGATTCACAATCAATTCTTAAGCTCACTACAGACAATATCAAAGCCATAATCATGGTAAATTTTCCACCCTATATATCTATTATTGTCCTTTTAGGAAGAAAAAAAAAAAAAAAAGTTTTTTGGATCTAATTTCTATTCAGTTTCTACGTTTCAAAACATGATCTTTGAGTTTAATTTCAATTTACTCTTGAAAGAGACCTAAGTTTTGTTTTAATTTGGTTACTAGCTAGGTTTCAAAATTTACGTTTTTAACATTCAATTTTCACTATGTACTCCTTTTTTTTTTTTTTTTTAATATCTATGGTTTTATTTAAAATAATTATGAAGTTTATATATGAATTATATATAATATATAATTTTAATTTTAATTTTAACTTCATAATTATGTTAAATAAAATAATAAATATCAACACTTTCCTTCTAAAATTGAAAAGATTATACAATTATTAGTTTAAAATATGTTATTTTCAAAATAATATTTAATATATTCTTCATCTTTCAATTTAGTGTAAAGTGCTTGAAATTTGAAAATGGTCTAATAATGATGCGAAGGTTCTTAAATTTTTTTTTTTTTTAATCTAATAGATATATGAAATTTTAATTTTGTGTTTAATAGACCATTGATCAATTTTACACTTTTAACGTCATAGACTTACTACACATAAAATTTTAAAATAATTTTATTAGATATAAAATTGAGTTTTATTAAAATTTCAAATATGTTATGACTTATTAGACACTAGATTAAAAATTTTGAGATTAATTAGACACAAATTTAAAATACATATACCTATTAGAAAATTTTTAAGACAAATGTATTAGACAAAAACATGAAAATAATAATACAAACTTGTAGTCGAACAAATATTCAAAGTTATTTTATATGAGGCTAAGGTCTTAAATGAGAAACACTTGAAATAAATTAATTACAAATATTAATCGTTTTATTTTTTCTTTTTTGTTTTCTGCTAAATTTCAATTAAAATAACACACACATATATATATATTATTTTTTTTAAAAGACTTAAAAATTATATTTTGATTATGCTGATTTTGTTAAAATTTAAAAATTTTCTAAGAATTGAAAATATTTACTCATTTAGAGAAGAATAATATACATAGTACATATTATACTAAAATAGAAATGGGTCTAAAATTTATGGTTGAATATGAGCTAGAAACTAAAAGAAAATAAAAAAGTTTTAAATAGTATTTATCGATTTTATTTTGATTTTTAAACTTTAAACTTTTGTTATTTTAATTTCTATTATTTCTAAAATATCTATTTTGGTTCTATAATTTTAAGTCACATTTTCCCATTAGAAAATACCGAAGTCATACTTTAATCTTCTATTTGTAAAATTTTATATTCAATATAACTCTTTAAAATAAATTTAAGTTACATTTTAATCCTCATAAAATTTCTAGTGTCCATTAGTGCTAATCAATATCTTTTATAAATATACGTAACATATTAATTTTAATAAAGAATACTAAAAAAACATAATTATTAATTTTCTGATTATTTTTCATTAGGATGTAATGTTCGGTGGAACGGAAACAGTAACTTCAGTGATAGAATGGGCAATGGCGGAGTTAATGAAAAATCCCGAAGAGCCCAATAAGGTCCAACAAGAACTCACACTGACTGTTGGATTAGATCGGACAGTCCACGAATCGGACCTCCAAAATCTCCCCTACTTAAAATTTGCCATCACAGAAACCCTCCGCCTTCATCCGCCAATTCCACTGCTCCTCCACGAGACTGCTGTTAACACCTCCGTCGCCGGCTACTTCATTCCAACCGGTTCAAGAGTCTGGATCAACGCTTGGGCAATCGGTCGAGACCGAACCGCTTGGGAAGAACCGGACCGTTTCTGGCCAGGGAGGTTTCAAAGGAAGCAATTTCGAGTTCATCCCGTTCGGGTCGGGTCGGCGATCTTGTCCAGGAATGCAACTTGGACTTTATGCTTGTGAGATGGCGGTTGCAAATCTTGTTCATTGCTTTTCGTGGAAATTGCCGGACGGAATGAATGCCAATGAGCTCGATATGAATGACTCGTTCGGACTCACTGCTCCAAGAGCGATTCGACTCGTAGCTGTACCGGGTTTTCGACTTAACTCGCCTGAGTTTGAACTCGAAGGGAAGAGACGGGAAGATGTATGA

mRNA sequence

TTTACTCATTATTTATTGGCATTCCATATAAGTGCGCTACATCCATACCTCCCTAACTGAAGTTCATAGAATCGAATGCTACATTTTAGAAGTAAGTACATAAGGCATAAGCAACCTCCTTTTAAAAATAAATTTACGACATTTTTTCAAGAAAGTTACACACTTGTTATTTATTGTAATAAATTGTCACAAACCTTTGAATTAAAAATATATAACAAGGCAGAGTTATCAGTTTACTAAATTAAGATATCATACTCCCTCCCATCAATTTCCCTTTCTCAAATTCATGCTCTCAAAAGACAAAACCAAAATCCTTCATCTCTATAAATTCAATATTCCTCTCTAAATCCATTCAAATTCAAACACTATCATACGTCAACTCAATGGAGTTTCTAAACCCTTCTTCTTTTCTCTTCTTCTCTTTGTCGCTTTTTTGCGAAATTTACATCGTAAATTTAGCTACCCGCCTGGCCCCGGTGGATTGCCCATTATCGGCAACATGCTGATGATGGGCCAACTCCCCCACCGTGGCCTCGCCCGTCTCGCCGCCATCTACGGTGGCCTCTTCCACCTTCGACTCGGCGTCCTCCACATGGTGGTTGTCTCCACGCCAGATATGGCACGAGAATTCCTCCAAGTTCAAGACGCAGCGTTCGCAAATAGGGCTGCAAACGTTGCGATCACATATCTCACCTACGATCGCGCCGATATGGCCTTCGCCAACTACGGCCCATTTTGGCGCCAAATGCGAAAGATTTGCGTCATCAAGCTCTTCAGCCGCCGACGCGCAGAGTCATGGGCGTCGGTGCGTGACGAAGTCGACGCCCTAGTCCGAATGGTTTCAAGTAAAATGGGGCAACCGGTGAATATCGGTGACCTAGTTTTCAGCCTGACACGAAACATAACCTACAAGGCCGCGTTTGGATCGAGGTCGCACGAAGGACAGGATGAGTTGGTTAAGATCTTGCAAGAATTCTCTAAACTGTTTGGAGCTTTTAATATTGCACATTTGTTGCCATGGTTGGGATGGATTCATGCTCGAGATTTCAATGAAAGAATGGCAAAAGCTAGAAGAGATCTTGCTGTGTTCATAAATACCATAATTGATGAACATTTACAAAAGAAAAGGAAGAAAAAGGGAAAAGATGAAGATGATGAAGAAGAAGATTCAGATATGGTTGATGAATTAATGGCATTCCTTGCTGATGAGTCAAGTAACAATGAGTTCGATGATTCACAATCAATTCTTAAGCTCACTACAGACAATATCAAAGCCATAATCATGGATGTAATGTTCGGTGGAACGGAAACAGTAACTTCAGTGATAGAATGGGCAATGGCGGAGTTAATGAAAAATCCCGAAGAGCCCAATAAGAAACCCTCCGCCTTCATCCGCCAATTCCACTGCTCCTCCACGAGACTGCTGTTAACACCTCCGTCGCCGGCTACTTCATTCCAACCGGTTCAAGAGTCTGGATCAACGCTTGGGCAATCGGTCGAGACCGAACCGCTTGGGAAGAACCGGACCGTTTCTGGCCAGGGAGGTTTCAAAGGAAGCAATTTCGAGTTCATCCCGTTCGGGTCGGGTCGGCGATCTTGTCCAGGAATGCAACTTGGACTTTATGCTTGTGAGATGGCGGTTGCAAATCTTGTTCATTGCTTTTCGTGGAAATTGCCGGACGGAATGAATGCCAATGAGCTCGATATGAATGACTCGTTCGGACTCACTGCTCCAAGAGCGATTCGACTCGTAGCTGTACCGGGTTTTCGACTTAACTCGCCTGAGTTTGAACTCGAAGGGAAGAGACGGGAAGATGTATGA

Coding sequence (CDS)

ATGCTGATGATGGGCCAACTCCCCCACCGTGGCCTCGCCCGTCTCGCCGCCATCTACGGTGGCCTCTTCCACCTTCGACTCGGCGTCCTCCACATGGTGGTTGTCTCCACGCCAGATATGGCACGAGAATTCCTCCAAGTTCAAGACGCAGCGTTCGCAAATAGGGCTGCAAACGTTGCGATCACATATCTCACCTACGATCGCGCCGATATGGCCTTCGCCAACTACGGCCCATTTTGGCGCCAAATGCGAAAGATTTGCGTCATCAAGCTCTTCAGCCGCCGACGCGCAGAGTCATGGGCGTCGGTGCGTGACGAAGTCGACGCCCTAGTCCGAATGGTTTCAAGTAAAATGGGGCAACCGGTGAATATCGGTGACCTAGTTTTCAGCCTGACACGAAACATAACCTACAAGGCCGCGTTTGGATCGAGGTCGCACGAAGGACAGGATGAGTTGGTTAAGATCTTGCAAGAATTCTCTAAACTGTTTGGAGCTTTTAATATTGCACATTTGTTGCCATGGTTGGGATGGATTCATGCTCGAGATTTCAATGAAAGAATGGCAAAAGCTAGAAGAGATCTTGCTGTGTTCATAAATACCATAATTGATGAACATTTACAAAAGAAAAGGAAGAAAAAGGGAAAAGATGAAGATGATGAAGAAGAAGATTCAGATATGGTTGATGAATTAATGGCATTCCTTGCTGATGAGTCAAGTAACAATGAGTTCGATGATTCACAATCAATTCTTAAGCTCACTACAGACAATATCAAAGCCATAATCATGGATGTAATGTTCGGTGGAACGGAAACAGTAACTTCAGTGATAGAATGGGCAATGGCGGAGTTAATGAAAAATCCCGAAGAGCCCAATAAGAAACCCTCCGCCTTCATCCGCCAATTCCACTGCTCCTCCACGAGACTGCTGTTAACACCTCCGTCGCCGGCTACTTCATTCCAACCGGTTCAAGAGTCTGGATCAACGCTTGGGCAATCGGTCGAGACCGAACCGCTTGGGAAGAACCGGACCGTTTCTGGCCAGGGAGGTTTCAAAGGAAGCAATTTCGAGTTCATCCCGTTCGGGTCGGGTCGGCGATCTTGTCCAGGAATGCAACTTGGACTTTATGCTTGTGAGATGGCGGTTGCAAATCTTGTTCATTGCTTTTCGTGGAAATTGCCGGACGGAATGAATGCCAATGAGCTCGATATGAATGACTCGTTCGGACTCACTGCTCCAAGAGCGATTCGACTCGTAGCTGTACCGGGTTTTCGACTTAACTCGCCTGAGTTTGAACTCGAAGGGAAGAGACGGGAAGATGTATGA

Protein sequence

MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVAITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQPVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHARDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSNNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFIRQFHCSSTRLLLTPPSPATSFQPVQESGSTLGQSVETEPLGKNRTVSGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMAVANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEFELEGKRREDV
Homology
BLAST of CcUC02G022300 vs. NCBI nr
Match: XP_038888732.1 (cytochrome P450 84A1-like [Benincasa hispida])

HSP 1 Score: 664.5 bits (1713), Expect = 6.5e-187
Identity = 360/482 (74.69%), Postives = 382/482 (79.25%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGLA LA IYGGLFHLRLGVLHM+VVSTP++AREFLQVQD AFANR ANVA
Sbjct: 45  MLMMDQLTHRGLAHLATIYGGLFHLRLGVLHMMVVSTPNVAREFLQVQDIAFANRPANVA 104

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           ITYLTYDRAD+AFANYGPFWRQMRKICVIKLFSRRR ESWASVRDEVDALV+MVSSK+ Q
Sbjct: 105 ITYLTYDRADVAFANYGPFWRQMRKICVIKLFSRRRTESWASVRDEVDALVQMVSSKIRQ 164

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIGDLVFSLTRNITYKAAFGS SHEGQDE +KILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 165 PVNIGDLVFSLTRNITYKAAFGSSSHEGQDEFIKILQEFSKLFGAFNIADFLPWLGWIHA 224

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKK-KGKDEDDEEEDSDMVDELMAFLADE-S 240
           RDFNERMAKARRDL VFI++IIDEHLQKK KK KGK ED+EE DSDMVDELMAFLADE S
Sbjct: 225 RDFNERMAKARRDLDVFIDSIIDEHLQKKMKKLKGKKEDEEEADSDMVDELMAFLADESS 284

Query: 241 SNNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFI 300
           SNNEFDDSQS+LKLT DNIKA+IMDVMFGGTETV SVIEWAMAELMKNPEE  K      
Sbjct: 285 SNNEFDDSQSVLKLTRDNIKALIMDVMFGGTETVASVIEWAMAELMKNPEELKKVQQELT 344

Query: 301 ------RQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE---- 360
                 R  H S               L L PP P    +   +S S  G  + T     
Sbjct: 345 LTVGLDRMVHESDLEHLPYLKFAVKETLRLHPPIPLLLHETAADS-SVAGYFIPTGSRVW 404

Query: 361 ----PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACE 420
                +G++RT                    FKGS+FEFIPFGSGRRSCPGMQLGLYACE
Sbjct: 405 INAWAIGRDRTAWEEPDRFWPGRFQNDTAPDFKGSDFEFIPFGSGRRSCPGMQLGLYACE 464

Query: 421 MAVANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEFELEGKRRE 441
           MAVA+LVHCFSWKLPDGM A+ELDM+DSFGLTAPRAIRLVAVP FRLNSPEFELEGKRRE
Sbjct: 465 MAVASLVHCFSWKLPDGMKADELDMSDSFGLTAPRAIRLVAVPSFRLNSPEFELEGKRRE 524

BLAST of CcUC02G022300 vs. NCBI nr
Match: KAA0033772.1 (cytochrome P450 84A1-like [Cucumis melo var. makuwa] >TYK22363.1 cytochrome P450 84A1-like [Cucumis melo var. makuwa])

HSP 1 Score: 651.4 bits (1679), Expect = 5.7e-183
Identity = 350/481 (72.77%), Postives = 379/481 (78.79%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGLA LA IYGGLFHLRLG+LHMVVVSTPDMAREFLQVQD AFANR ANVA
Sbjct: 1   MLMMDQLTHRGLAHLATIYGGLFHLRLGILHMVVVSTPDMAREFLQVQDIAFANRPANVA 60

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFANYGPFWRQMRKICV+KLFSRRR ESWASVRDEVDAL+++VSSK+ Q
Sbjct: 61  ISYLTYDRADMAFANYGPFWRQMRKICVVKLFSRRRTESWASVRDEVDALIKIVSSKIQQ 120

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIGDLVFSLTRNITYKAAFGS SHEGQDE VKILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 121 PVNIGDLVFSLTRNITYKAAFGSSSHEGQDEFVKILQEFSKLFGAFNIADFLPWLGWIHA 180

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKK-KGKDEDDEEEDSDMVDELMAFLADESS 240
           R+FNER+AKARRDL +FI++IIDEHLQKK KK KGK  DD EEDSDMVDELMAFL D+SS
Sbjct: 181 REFNERLAKARRDLDMFIDSIIDEHLQKKMKKLKGKTVDD-EEDSDMVDELMAFLVDDSS 240

Query: 241 NNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFI- 300
           +NEFDDSQS+LKLT DNIKA+IMDVMFGGTETV SVIEWAMAELMKNPEE NK       
Sbjct: 241 SNEFDDSQSVLKLTRDNIKALIMDVMFGGTETVASVIEWAMAELMKNPEELNKLQQELTL 300

Query: 301 -----RQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE----- 360
                R  H S  +            L L PP P    +   +S S  G  + T      
Sbjct: 301 TVGLDRNVHESDLQNLPYLKFVIKETLRLHPPIPLLLHETAVDS-SVDGYFIPTGSRVWI 360

Query: 361 ---PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEM 420
               +G++RT                    FKG++FEF+PFGSGRRSCPGMQLGLYACEM
Sbjct: 361 NAWAIGRDRTAWEEPDRFRPGRFQNDAAPDFKGNDFEFVPFGSGRRSCPGMQLGLYACEM 420

Query: 421 AVANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEFELEGKRRED 441
           AVANLVHCFSW+LP GM A+ELDMNDSFGLTAPRAIRLVAVP  RLNSPEF+LE KRRED
Sbjct: 421 AVANLVHCFSWELPGGMTADELDMNDSFGLTAPRAIRLVAVPSLRLNSPEFKLERKRRED 479

BLAST of CcUC02G022300 vs. NCBI nr
Match: XP_008448126.1 (PREDICTED: cytochrome P450 84A1-like [Cucumis melo])

HSP 1 Score: 651.0 bits (1678), Expect = 7.5e-183
Identity = 350/481 (72.77%), Postives = 379/481 (78.79%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGLA LA IYGGLFHLRLG+LHMVVVSTPDMAREFLQVQD AFANR ANVA
Sbjct: 45  MLMMDQLTHRGLAHLATIYGGLFHLRLGILHMVVVSTPDMAREFLQVQDIAFANRPANVA 104

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFANYGPFWRQMRKICV+KLFSRRR ESWASVRDEVDAL+++VSSK+ Q
Sbjct: 105 ISYLTYDRADMAFANYGPFWRQMRKICVVKLFSRRRIESWASVRDEVDALIKIVSSKIQQ 164

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIGDLVFSLTRNITYKAAFGS SHEGQDE VKILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 165 PVNIGDLVFSLTRNITYKAAFGSSSHEGQDEFVKILQEFSKLFGAFNIADFLPWLGWIHA 224

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKK-KGKDEDDEEEDSDMVDELMAFLADESS 240
           R+FNER+AKARRDL +FI++IIDEHLQKK KK KGK  DD EEDSDMVDELMAFL D+SS
Sbjct: 225 REFNERLAKARRDLDMFIDSIIDEHLQKKMKKLKGKTVDD-EEDSDMVDELMAFLVDDSS 284

Query: 241 NNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFI- 300
           +NEFDDSQS+LKLT DNIKA+IMDVMFGGTETV SVIEWAMAELMKNPEE NK       
Sbjct: 285 SNEFDDSQSVLKLTRDNIKALIMDVMFGGTETVASVIEWAMAELMKNPEELNKLQQELTL 344

Query: 301 -----RQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE----- 360
                R  H S  +            L L PP P    +   +S S  G  + T      
Sbjct: 345 TVGLDRNVHESDLQNLPYLKFVIKETLRLHPPIPLLLHETAVDS-SVDGYFIPTGSRVWI 404

Query: 361 ---PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEM 420
               +G++RT                    FKG++FEF+PFGSGRRSCPGMQLGLYACEM
Sbjct: 405 NAWAIGRDRTAWEEPDRFRPGRFQNDAAPDFKGNDFEFVPFGSGRRSCPGMQLGLYACEM 464

Query: 421 AVANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEFELEGKRRED 441
           AVANLVHCFSW+LP GM A+ELDMNDSFGLTAPRAIRLVAVP  RLNSPEF+LE KRRED
Sbjct: 465 AVANLVHCFSWELPGGMTADELDMNDSFGLTAPRAIRLVAVPSLRLNSPEFKLERKRRED 523

BLAST of CcUC02G022300 vs. NCBI nr
Match: XP_004148555.1 (cytochrome P450 84A1 [Cucumis sativus] >KGN43170.1 hypothetical protein Csa_020542 [Cucumis sativus])

HSP 1 Score: 632.9 bits (1631), Expect = 2.1e-177
Identity = 340/471 (72.19%), Postives = 367/471 (77.92%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGLARLA IYGGLFHLRLGVLHMVVVSTPDMAREFLQVQD AFANR ANVA
Sbjct: 45  MLMMDQLTHRGLARLATIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDVAFANRPANVA 104

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVD LV +VS K+ Q
Sbjct: 105 ISYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDTLVEIVSRKIQQ 164

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIGDLVFSLTRNITYKAAFGS SHEGQDE VKILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 165 PVNIGDLVFSLTRNITYKAAFGSSSHEGQDEFVKILQEFSKLFGAFNIADFLPWLGWIHA 224

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSN 240
           R+FNER+AKARRDL VFI++IIDEHL+KK KK   ++ D+EED+DMVDELMAFL D+SS+
Sbjct: 225 REFNERLAKARRDLDVFIDSIIDEHLEKKMKKLKGEKVDDEEDTDMVDELMAFLVDDSSS 284

Query: 241 NEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFI-- 300
           NEFDDSQS+LKLT D+IKA+IMDVMFGGTETV SVIEWAMAELMKNPEE NK        
Sbjct: 285 NEFDDSQSVLKLTRDHIKALIMDVMFGGTETVASVIEWAMAELMKNPEELNKVQQELTLV 344

Query: 301 ----RQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE------ 360
               R  H S               L L PP P    +   +S S  G  + T       
Sbjct: 345 VGLDRNVHESDLENLPYLKFVVKETLRLHPPIPLLLHETAVDS-SVSGYFIPTGSRVWIN 404

Query: 361 --PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMA 420
              +G++RT                    FKGS+FEFIPFGSGRRSCPGMQLGLYACEMA
Sbjct: 405 AWAIGRDRTAWKEPDRFWPGRFQNDAAPDFKGSDFEFIPFGSGRRSCPGMQLGLYACEMA 464

Query: 421 VANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEFE 432
           VANLVHCFSW+LP GM A+ELDMNDSFGLTAPRAIRLVAVP  RLNSP+ E
Sbjct: 465 VANLVHCFSWELPGGMTADELDMNDSFGLTAPRAIRLVAVPSLRLNSPKEE 514

BLAST of CcUC02G022300 vs. NCBI nr
Match: KAG7022266.1 (Cytochrome P450 84A1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 577.4 bits (1487), Expect = 1.0e-160
Identity = 310/471 (65.82%), Postives = 356/471 (75.58%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM +L HRGL RLAAIYGGLFHLRLGVLHMVVVSTP+MAREFLQVQD AFANR ANVA
Sbjct: 46  MLMMNELTHRGLTRLAAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVA 105

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFANYGPFWRQMRKICV+KLFSRRRAESWASVRDEVD+++++VS+K+G+
Sbjct: 106 ISYLTYDRADMAFANYGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGE 165

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIG+LVF LTRNITYKAAFGS SHEGQDE V+ILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 166 PVNIGELVFGLTRNITYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHA 225

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSN 240
            +FN RM KAR+ L VFI++IIDEH+ KK   K  D D+++ DSDMVDELMAFL DESS+
Sbjct: 226 GEFNRRMEKARKSLDVFIDSIIDEHIAKK-LGKNDDNDEQDVDSDMVDELMAFLVDESSS 285

Query: 241 NEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNK------KP 300
           N+F       +LT DNIKAIIMDVMFGGTETV SVIEW+MAELM+NPEE  K      + 
Sbjct: 286 NDF-------QLTRDNIKAIIMDVMFGGTETVASVIEWSMAELMRNPEELQKLQEELTRT 345

Query: 301 SAFIRQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE------ 360
               R+ H S  +            L L PP P    +   ++ +  G  + T       
Sbjct: 346 VGLDRRLHESDLQNLPYLKAVVKETLRLHPPIPLLLHETAVDT-AVAGYFIPTGSRVWIN 405

Query: 361 --PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMA 420
              +G++ T                    FKG++FEF+PFGSGRRSCPGMQLGLYACEMA
Sbjct: 406 CWAIGRDGTAWEDPDRFRPGRFENDAAPDFKGNDFEFLPFGSGRRSCPGMQLGLYACEMA 465

Query: 421 VANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEFE 432
           VANL+H FSW+LPDGM A ELDMNDSFGLTAPRA+RLVAVP +RLNS  F+
Sbjct: 466 VANLLHSFSWELPDGMKAEELDMNDSFGLTAPRAVRLVAVPSYRLNSAGFK 507

BLAST of CcUC02G022300 vs. ExPASy Swiss-Prot
Match: Q42600 (Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.3e-134
Identity = 252/465 (54.19%), Postives = 323/465 (69.46%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGLA LA  YGGL HLR+G LHM  VS+P++AR+ LQVQD+ F+NR A +A
Sbjct: 53  MLMMDQLTHRGLANLAKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIA 112

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFA+YGPFWRQMRK+CV+K+FSR+RAESWASVRDEVD +VR VS  +G+
Sbjct: 113 ISYLTYDRADMAFAHYGPFWRQMRKVCVMKVFSRKRAESWASVRDEVDKMVRSVSCNVGK 172

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           P+N+G+ +F+LTRNITY+AAFGS   +GQDE ++ILQEFSKLFGAFN+A  +P+ GWI  
Sbjct: 173 PINVGEQIFALTRNITYRAAFGSACEKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDP 232

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESS- 240
           +  N+R+ KAR DL  FI+ IIDEH++KK  +   D D +  D+DMVD+L+AF ++E+  
Sbjct: 233 QGINKRLVKARNDLDGFIDDIIDEHMKKKENQNAVD-DGDVVDTDMVDDLLAFYSEEAKL 292

Query: 241 NNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAF-- 300
            +E  D Q+ +KLT DNIKAIIMDVMFGGTETV S IEWA+ EL+++PE+  +       
Sbjct: 293 VSETADLQNSIKLTRDNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAE 352

Query: 301 ---------------IRQFHCS-STRLLLTPPSPATSFQPVQESG-------STLGQSVE 360
                          +    C+    L + PP P    +  +++              + 
Sbjct: 353 VVGLDRRVEESDIEKLTYLKCTLKETLRMHPPIPLLLHETAEDTSIDGFFIPKKSRVMIN 412

Query: 361 TEPLGKNRT--------------VSGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMA 420
              +G++ T                G   FKGSNFEFIPFGSGRRSCPGMQLGLYA ++A
Sbjct: 413 AFAIGRDPTSWTDPDTFRPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLA 472

Query: 421 VANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRL 426
           VA+++HCF+WKLPDGM  +ELDMND FGLTAP+A RL AVP  RL
Sbjct: 473 VAHILHCFTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVPTTRL 516

BLAST of CcUC02G022300 vs. ExPASy Swiss-Prot
Match: F4JW83 (Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 5.5e-112
Identity = 228/473 (48.20%), Postives = 301/473 (63.64%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           +LMM Q  HRGLA+L+ IYGGL HLRLG  H+ VVS+PD+AR+ LQVQD  F+NR   +A
Sbjct: 45  ILMMNQFNHRGLAKLSRIYGGLLHLRLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIA 104

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I YLTY  +D+AF NYGPFWR+MRK+ V+ LFSR+RAESW SV +EV   VR+V+S +G+
Sbjct: 105 IRYLTYGGSDLAFCNYGPFWRRMRKLYVMMLFSRKRAESWVSVDEEVHKSVRLVASNVGK 164

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQ----DELVKILQEFSKLFGAFNIAHLLP-WL 180
           P+NI  L FSL+R+IT++AAFGS S        DE ++I+QEFSKLFG FN+A  +P WL
Sbjct: 165 PLNICKLAFSLSRDITFRAAFGSSSSTSDESRLDEFLEIIQEFSKLFGEFNVADYVPSWL 224

Query: 181 GWIHARDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLA 240
            WI  +  N R+ KAR+ L  FI ++ID+HL KK+    ++ D+ +E++DMVD+L+AF  
Sbjct: 225 SWIDPQGINGRVEKARKSLDGFIESVIDDHLHKKK----REHDNVDEETDMVDQLLAFYE 284

Query: 241 DESSNNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPS 300
           +E   N      S+ K+  DNIK IIMDVMFGGTETV   IEW + E++++PE   +   
Sbjct: 285 EEVKVN-----NSVTKINLDNIKGIIMDVMFGGTETVALAIEWVLTEILRSPENMKRVQD 344

Query: 301 AF----------IRQFHCSSTRLL---------LTPPSPATSFQPVQE---SGSTLGQS- 360
                       +   H      L         L PP P    + V++   SG  + +  
Sbjct: 345 ELTSVVGLDRWRVEDTHLEKLTFLKCILKETLRLHPPFPLLLHETVKDTEISGYFIPKGS 404

Query: 361 ---VETEPLGKN-RTVSGQGGF-------------KGSNFEFIPFGSGRRSCPGMQLGLY 420
              V T  LG++  + S    F             KG+NFEF+PFGSGRRSCPGMQLGLY
Sbjct: 405 RVMVNTYALGRDPNSWSDPESFNPGRFLNPIAPDLKGNNFEFVPFGSGRRSCPGMQLGLY 464

Query: 421 ACEMAVANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSP 429
           A E+AVA+L+HCF+W LPDGMN  ++D  +  GLT P+AI LVAVP  RL  P
Sbjct: 465 AFELAVAHLLHCFTWSLPDGMNPGDVDTVEGPGLTVPKAIPLVAVPTTRLLCP 508

BLAST of CcUC02G022300 vs. ExPASy Swiss-Prot
Match: A6YIH8 (Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 7.5e-61
Identity = 158/462 (34.20%), Postives = 237/462 (51.30%), Query Frame = 0

Query: 3   MMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVAIT 62
           M+G LPH  L  LA  YG L HL+LG +  VVV++PDMA+E L+  D AFA+R   +A  
Sbjct: 48  MVGGLPHHVLRDLAKKYGPLMHLQLGEVSAVVVTSPDMAKEVLKTHDIAFASRPKLLAPE 107

Query: 63  YLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASV-RDEVDALVRMVSSKMGQP 122
            + Y+R+D+AF  YG +WRQMRKICV+++ S +   S++S+ RDEV  LV  V S   +P
Sbjct: 108 IVCYNRSDIAFCPYGDYWRQMRKICVLEVLSAKNVRSFSSIRRDEVLRLVNFVRSSTSEP 167

Query: 123 VNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHAR 182
           VN  + +F  T ++T ++AFG    E Q+  +++++E   L G F++A + P L ++H  
Sbjct: 168 VNFTERLFLFTSSMTCRSAFGKVFKE-QETFIQLIKEVIGLAGGFDVADIFPSLKFLHVL 227

Query: 183 DFNE-RMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSN 242
              E ++ KA   +   +  +I+EH  KK    GK  +      D++D L+  +      
Sbjct: 228 TGMEGKIMKAHHKVDAIVEDVINEH--KKNLAMGK-TNGALGGEDLIDVLLRLM------ 287

Query: 243 NEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFIRQ 302
              +D      +T DNIKAII D+   GTET +S + WAM ++M+NP    K  +     
Sbjct: 288 ---NDGGLQFPITNDNIKAIIFDMFAAGTETSSSTLVWAMVQMMRNPTILAKAQAEVREA 347

Query: 303 FHCSST------------------RLLLTPPSPATSFQPVQESGSTLGQSVETE------ 362
           F    T                   L L PP P    +  +E     G ++  +      
Sbjct: 348 FKGKETFDENDVEELKYLKLVIKETLRLHPPVPLLVPRECREETEINGYTIPVKTKVMVN 407

Query: 363 --PLGKN-------------RTVSGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMAV 422
              LG++             R       F G+NFE++PFG GRR CPG+  GL    + +
Sbjct: 408 VWALGRDPKYWDDADNFKPERFEQCSVDFIGNNFEYLPFGGGRRICPGISFGLANVYLPL 467

BLAST of CcUC02G022300 vs. ExPASy Swiss-Prot
Match: O22307 (Cytochrome P450 71D11 (Fragment) OS=Lotus japonicus OX=34305 GN=CYP71D11 PE=2 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 5.4e-59
Identity = 147/460 (31.96%), Postives = 233/460 (50.65%), Query Frame = 0

Query: 3   MMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVAIT 62
           ++G  PHR L  LA  YG L HL+LG +  ++VS+ + A+E ++  D  FA+R  ++   
Sbjct: 35  LVGSPPHRKLRDLAKKYGPLMHLQLGEVIFIIVSSAEYAKEVMKTHDVTFASRPRSLFTD 94

Query: 63  YLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAES-WASVRDEVDALVRMVSSKMGQP 122
            + Y   D+ F+ YG +WRQ+RKIC ++L S +R +S W    +EV  L++ ++S+ G  
Sbjct: 95  IVFYGSTDIGFSPYGDYWRQVRKICNVELLSMKRVQSLWPIREEEVKNLIQRIASEEGSV 154

Query: 123 VNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWI-HA 182
           VN+   + SL   IT ++AFG R  E Q+E +  ++E  KL G FNIA L P   W+ + 
Sbjct: 155 VNLSQAIDSLIFTITSRSAFGKRYME-QEEFISCVREVMKLAGGFNIADLFPSAKWLENL 214

Query: 183 RDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSN 242
                +     + +   + TIID+H    R K+G+ E  EE   D++D L+ +   E+S+
Sbjct: 215 TRMRSKFEYLHQKMDRILETIIDDHKANSRTKEGQVEGGEE---DLIDVLLKY---ENSS 274

Query: 243 NEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNP----EEPNKKPSA 302
            + D       LT  NIKAI+ D+   G+ET  + I W MAE+MK+P    +  ++    
Sbjct: 275 TDQD-----FHLTIRNIKAILFDIFIAGSETSATTINWTMAEMMKDPILLKKAQDEVREI 334

Query: 303 FIRQFHCSST--------------RLLLTPPSPATSFQPVQESGSTLGQSV--------- 362
           F R+     T               L L PP P   F+  +++    G  +         
Sbjct: 335 FQRRGKVDETCIYELKYLKAFINEVLRLHPPGPLV-FRECRQACEINGYHIPAKSTVLVN 394

Query: 363 ------------ETEPLGKNRTVSGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMAV 422
                       E E     R +     +KG+NFE +PFG+GRR CPG+  G+   E+ +
Sbjct: 395 TFAIGTDSKYWAEPERFCPERFIDSSIDYKGTNFEHLPFGAGRRICPGINYGMANVELVL 454

BLAST of CcUC02G022300 vs. ExPASy Swiss-Prot
Match: A0A0N9HT29 (Desmethyl-deoxy-podophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71BE54 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.7e-57
Identity = 150/457 (32.82%), Postives = 241/457 (52.74%), Query Frame = 0

Query: 7   LPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVAITYLTY 66
           LPH  L  LA  +G L HL+LG    +VVS+  MAR  +   D  FANR    A   L Y
Sbjct: 53  LPHHALRNLAKKHGPLMHLQLGENSTLVVSSSKMARAIMSTHDLMFANRTVQFATDILLY 112

Query: 67  DRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVR-DEVDALVRMVSSKMGQPVNIG 126
               +  A YG +WRQ+RKICV++L S +R +S+ +VR +E+  L+R +S++  + VN  
Sbjct: 113 GGKGIGLAPYGDYWRQIRKICVLELLSAKRVQSFQTVREEEISNLIRSISAESSK-VNFS 172

Query: 127 DLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHARDFNE 186
           +++ SLT +I  +AAFG +  + +   + +++E  +L G F+IA L P L  +H     +
Sbjct: 173 EMITSLTNDIIARAAFGEKCKD-KHAFLSLIEEGIQLAGGFDIAGLFPSLKLLHVITGKK 232

Query: 187 R-MAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSNNEFD 246
           R + +   +L   +  II E+ +K+R  K  +ED  EE  ++VD L+            +
Sbjct: 233 RQLERVHNELDRILVDIIKENKEKRRASK-LNEDKYEE--NLVDVLVRVQ---------E 292

Query: 247 DSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFIRQFHCS 306
           ++Q  + L  DN+KA+I+D+   G+ET ++ IEWAM+E++KNP +  KK  A +R+    
Sbjct: 293 NTQLEVPLANDNLKAVILDIFVAGSETSSTTIEWAMSEMLKNP-KVMKKAQAEVRRVFSG 352

Query: 307 STR-------------------LLLTPPSPATSFQPVQESGSTLGQSVETE--------P 366
           + +                   L L  P+P    +  +ES    G  +  +         
Sbjct: 353 NKKINETEIQELSYLKLVLKETLRLHAPAPLLIPRECRESCEIDGYEIPKKTMVMVNAWA 412

Query: 367 LGKNRTVSGQG-------------GFKGSNFEFIPFGSGRRSCPGMQLGLYACEMAVANL 422
           +G++      G              +KG+NFE+IPFG+GRR CPGM  G+   E+ +A L
Sbjct: 413 IGRDPENWSNGDRFEPERFDGISVDYKGTNFEYIPFGAGRRICPGMLFGMANVEVPLARL 472

BLAST of CcUC02G022300 vs. ExPASy TrEMBL
Match: A0A5A7SRE0 (Cytochrome P450 84A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1428G00850 PE=3 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.8e-183
Identity = 350/481 (72.77%), Postives = 379/481 (78.79%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGLA LA IYGGLFHLRLG+LHMVVVSTPDMAREFLQVQD AFANR ANVA
Sbjct: 1   MLMMDQLTHRGLAHLATIYGGLFHLRLGILHMVVVSTPDMAREFLQVQDIAFANRPANVA 60

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFANYGPFWRQMRKICV+KLFSRRR ESWASVRDEVDAL+++VSSK+ Q
Sbjct: 61  ISYLTYDRADMAFANYGPFWRQMRKICVVKLFSRRRTESWASVRDEVDALIKIVSSKIQQ 120

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIGDLVFSLTRNITYKAAFGS SHEGQDE VKILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 121 PVNIGDLVFSLTRNITYKAAFGSSSHEGQDEFVKILQEFSKLFGAFNIADFLPWLGWIHA 180

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKK-KGKDEDDEEEDSDMVDELMAFLADESS 240
           R+FNER+AKARRDL +FI++IIDEHLQKK KK KGK  DD EEDSDMVDELMAFL D+SS
Sbjct: 181 REFNERLAKARRDLDMFIDSIIDEHLQKKMKKLKGKTVDD-EEDSDMVDELMAFLVDDSS 240

Query: 241 NNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFI- 300
           +NEFDDSQS+LKLT DNIKA+IMDVMFGGTETV SVIEWAMAELMKNPEE NK       
Sbjct: 241 SNEFDDSQSVLKLTRDNIKALIMDVMFGGTETVASVIEWAMAELMKNPEELNKLQQELTL 300

Query: 301 -----RQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE----- 360
                R  H S  +            L L PP P    +   +S S  G  + T      
Sbjct: 301 TVGLDRNVHESDLQNLPYLKFVIKETLRLHPPIPLLLHETAVDS-SVDGYFIPTGSRVWI 360

Query: 361 ---PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEM 420
               +G++RT                    FKG++FEF+PFGSGRRSCPGMQLGLYACEM
Sbjct: 361 NAWAIGRDRTAWEEPDRFRPGRFQNDAAPDFKGNDFEFVPFGSGRRSCPGMQLGLYACEM 420

Query: 421 AVANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEFELEGKRRED 441
           AVANLVHCFSW+LP GM A+ELDMNDSFGLTAPRAIRLVAVP  RLNSPEF+LE KRRED
Sbjct: 421 AVANLVHCFSWELPGGMTADELDMNDSFGLTAPRAIRLVAVPSLRLNSPEFKLERKRRED 479

BLAST of CcUC02G022300 vs. ExPASy TrEMBL
Match: A0A1S3BID8 (cytochrome P450 84A1-like OS=Cucumis melo OX=3656 GN=LOC103490415 PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 3.6e-183
Identity = 350/481 (72.77%), Postives = 379/481 (78.79%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGLA LA IYGGLFHLRLG+LHMVVVSTPDMAREFLQVQD AFANR ANVA
Sbjct: 45  MLMMDQLTHRGLAHLATIYGGLFHLRLGILHMVVVSTPDMAREFLQVQDIAFANRPANVA 104

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFANYGPFWRQMRKICV+KLFSRRR ESWASVRDEVDAL+++VSSK+ Q
Sbjct: 105 ISYLTYDRADMAFANYGPFWRQMRKICVVKLFSRRRIESWASVRDEVDALIKIVSSKIQQ 164

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIGDLVFSLTRNITYKAAFGS SHEGQDE VKILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 165 PVNIGDLVFSLTRNITYKAAFGSSSHEGQDEFVKILQEFSKLFGAFNIADFLPWLGWIHA 224

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKK-KGKDEDDEEEDSDMVDELMAFLADESS 240
           R+FNER+AKARRDL +FI++IIDEHLQKK KK KGK  DD EEDSDMVDELMAFL D+SS
Sbjct: 225 REFNERLAKARRDLDMFIDSIIDEHLQKKMKKLKGKTVDD-EEDSDMVDELMAFLVDDSS 284

Query: 241 NNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFI- 300
           +NEFDDSQS+LKLT DNIKA+IMDVMFGGTETV SVIEWAMAELMKNPEE NK       
Sbjct: 285 SNEFDDSQSVLKLTRDNIKALIMDVMFGGTETVASVIEWAMAELMKNPEELNKLQQELTL 344

Query: 301 -----RQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE----- 360
                R  H S  +            L L PP P    +   +S S  G  + T      
Sbjct: 345 TVGLDRNVHESDLQNLPYLKFVIKETLRLHPPIPLLLHETAVDS-SVDGYFIPTGSRVWI 404

Query: 361 ---PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEM 420
               +G++RT                    FKG++FEF+PFGSGRRSCPGMQLGLYACEM
Sbjct: 405 NAWAIGRDRTAWEEPDRFRPGRFQNDAAPDFKGNDFEFVPFGSGRRSCPGMQLGLYACEM 464

Query: 421 AVANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEFELEGKRRED 441
           AVANLVHCFSW+LP GM A+ELDMNDSFGLTAPRAIRLVAVP  RLNSPEF+LE KRRED
Sbjct: 465 AVANLVHCFSWELPGGMTADELDMNDSFGLTAPRAIRLVAVPSLRLNSPEFKLERKRRED 523

BLAST of CcUC02G022300 vs. ExPASy TrEMBL
Match: A0A0A0K0F0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G004720 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 1.0e-177
Identity = 340/471 (72.19%), Postives = 367/471 (77.92%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGLARLA IYGGLFHLRLGVLHMVVVSTPDMAREFLQVQD AFANR ANVA
Sbjct: 45  MLMMDQLTHRGLARLATIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDVAFANRPANVA 104

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVD LV +VS K+ Q
Sbjct: 105 ISYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDTLVEIVSRKIQQ 164

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIGDLVFSLTRNITYKAAFGS SHEGQDE VKILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 165 PVNIGDLVFSLTRNITYKAAFGSSSHEGQDEFVKILQEFSKLFGAFNIADFLPWLGWIHA 224

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSN 240
           R+FNER+AKARRDL VFI++IIDEHL+KK KK   ++ D+EED+DMVDELMAFL D+SS+
Sbjct: 225 REFNERLAKARRDLDVFIDSIIDEHLEKKMKKLKGEKVDDEEDTDMVDELMAFLVDDSSS 284

Query: 241 NEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAFI-- 300
           NEFDDSQS+LKLT D+IKA+IMDVMFGGTETV SVIEWAMAELMKNPEE NK        
Sbjct: 285 NEFDDSQSVLKLTRDHIKALIMDVMFGGTETVASVIEWAMAELMKNPEELNKVQQELTLV 344

Query: 301 ----RQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE------ 360
               R  H S               L L PP P    +   +S S  G  + T       
Sbjct: 345 VGLDRNVHESDLENLPYLKFVVKETLRLHPPIPLLLHETAVDS-SVSGYFIPTGSRVWIN 404

Query: 361 --PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMA 420
              +G++RT                    FKGS+FEFIPFGSGRRSCPGMQLGLYACEMA
Sbjct: 405 AWAIGRDRTAWKEPDRFWPGRFQNDAAPDFKGSDFEFIPFGSGRRSCPGMQLGLYACEMA 464

Query: 421 VANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEFE 432
           VANLVHCFSW+LP GM A+ELDMNDSFGLTAPRAIRLVAVP  RLNSP+ E
Sbjct: 465 VANLVHCFSWELPGGMTADELDMNDSFGLTAPRAIRLVAVPSLRLNSPKEE 514

BLAST of CcUC02G022300 vs. ExPASy TrEMBL
Match: A0A6J1I197 (cytochrome P450 84A1-like OS=Cucurbita maxima OX=3661 GN=LOC111469596 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 6.6e-161
Identity = 311/470 (66.17%), Postives = 355/470 (75.53%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGL RLAAIYGGLFHLRLGVLHMVVVSTP+MAREFLQVQD AFANR ANVA
Sbjct: 46  MLMMNQLTHRGLTRLAAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVA 105

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFANYGPFWRQMRKICV+KLFSRRRAESWASVRDEVD+++++VS+K+G+
Sbjct: 106 ISYLTYDRADMAFANYGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGE 165

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIG+LVF LTRNITYKAAFGS SHEGQDE V+ILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 166 PVNIGELVFGLTRNITYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHA 225

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSN 240
            +FN RM KAR+ L VFI++IIDEH+ KK   K  D D+++ DSDMVDELMAFL DESS+
Sbjct: 226 GEFNRRMEKARKALDVFIDSIIDEHIAKK-LGKNDDNDEQDVDSDMVDELMAFLVDESSS 285

Query: 241 NEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNK------KP 300
           N+F       +LT DNIKAIIMDVMFGGTETV SVIEW++AELM+NPEE  K      + 
Sbjct: 286 NDF-------QLTRDNIKAIIMDVMFGGTETVASVIEWSIAELMRNPEELQKLQEELTRT 345

Query: 301 SAFIRQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE------ 360
               R+ H S  +            L L PP P    +   ++ +  G  + T       
Sbjct: 346 VGLDRKLHESDLQNLPYLKAVVKETLRLHPPIPLLLHETAVDT-AVAGYLIPTGSRVWIN 405

Query: 361 --PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMA 420
              +G++ T                    FKGS+FEF+PFGSGRRSCPGMQLGLYACEMA
Sbjct: 406 CWAIGRDGTAWEDPDRFRPGRFENDAAPDFKGSDFEFLPFGSGRRSCPGMQLGLYACEMA 465

Query: 421 VANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEF 431
           VANL+H FSW+LPDGM A ELDMNDSFGLTAPRA+RLVAVP +RLNS  F
Sbjct: 466 VANLLHSFSWELPDGMKAEELDMNDSFGLTAPRAVRLVAVPSYRLNSAGF 506

BLAST of CcUC02G022300 vs. ExPASy TrEMBL
Match: A0A6J1F4G7 (cytochrome P450 84A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440398 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 6.6e-161
Identity = 310/470 (65.96%), Postives = 355/470 (75.53%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM +L HRGL RLAAIYGGLFHLRLGVLHMVVVSTP+MAREFLQVQD AFANR ANVA
Sbjct: 45  MLMMNELTHRGLTRLAAIYGGLFHLRLGVLHMVVVSTPEMAREFLQVQDIAFANRPANVA 104

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFANYGPFWRQMRKICV+KLFSRRRAESWASVRDEVD+++++VS+K+G+
Sbjct: 105 ISYLTYDRADMAFANYGPFWRQMRKICVMKLFSRRRAESWASVRDEVDSVLQIVSNKIGE 164

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           PVNIG+LVF LTRNITYKAAFGS SHEGQDE V+ILQEFSKLFGAFNIA  LPWLGWIHA
Sbjct: 165 PVNIGELVFGLTRNITYKAAFGSCSHEGQDEFVEILQEFSKLFGAFNIADFLPWLGWIHA 224

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSN 240
            +FN RM KAR+ L VFI++IIDEH+ KK   K  D D+++ DSDMVDELMAFL DESS+
Sbjct: 225 GEFNRRMEKARKSLDVFIDSIIDEHIAKK-LGKNDDNDEQDVDSDMVDELMAFLVDESSS 284

Query: 241 NEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNK------KP 300
           N+F       +LT DNIKAIIMDVMFGGTETV SVIEW+MAELM+NPEE  K      + 
Sbjct: 285 NDF-------QLTRDNIKAIIMDVMFGGTETVASVIEWSMAELMRNPEELQKLQEELTRT 344

Query: 301 SAFIRQFHCSSTR------------LLLTPPSPATSFQPVQESGSTLGQSVETE------ 360
               R+ H S  +            L L PP P    +   ++ +  G  + T       
Sbjct: 345 VGLDRRLHESDLQNLPYLKAVVKETLRLHPPIPLLLHETAVDT-AVAGYFIPTGSRVWIN 404

Query: 361 --PLGKNRTV--------------SGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMA 420
              +G++ T                    FKG++FEF+PFGSGRRSCPGMQLGLYACEMA
Sbjct: 405 CWAIGRDGTAWEDPDRFRPGRFENDAAPDFKGNDFEFLPFGSGRRSCPGMQLGLYACEMA 464

Query: 421 VANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSPEF 431
           VANL+H FSW+LPDGM A ELDMNDSFGLTAPRA+RLVAVP +RLNS  F
Sbjct: 465 VANLLHSFSWELPDGMKAEELDMNDSFGLTAPRAVRLVAVPSYRLNSAGF 505

BLAST of CcUC02G022300 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 481.1 bits (1237), Expect = 9.5e-136
Identity = 252/465 (54.19%), Postives = 323/465 (69.46%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           MLMM QL HRGLA LA  YGGL HLR+G LHM  VS+P++AR+ LQVQD+ F+NR A +A
Sbjct: 53  MLMMDQLTHRGLANLAKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIA 112

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I+YLTYDRADMAFA+YGPFWRQMRK+CV+K+FSR+RAESWASVRDEVD +VR VS  +G+
Sbjct: 113 ISYLTYDRADMAFAHYGPFWRQMRKVCVMKVFSRKRAESWASVRDEVDKMVRSVSCNVGK 172

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIHA 180
           P+N+G+ +F+LTRNITY+AAFGS   +GQDE ++ILQEFSKLFGAFN+A  +P+ GWI  
Sbjct: 173 PINVGEQIFALTRNITYRAAFGSACEKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDP 232

Query: 181 RDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESS- 240
           +  N+R+ KAR DL  FI+ IIDEH++KK  +   D D +  D+DMVD+L+AF ++E+  
Sbjct: 233 QGINKRLVKARNDLDGFIDDIIDEHMKKKENQNAVD-DGDVVDTDMVDDLLAFYSEEAKL 292

Query: 241 NNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPSAF-- 300
            +E  D Q+ +KLT DNIKAIIMDVMFGGTETV S IEWA+ EL+++PE+  +       
Sbjct: 293 VSETADLQNSIKLTRDNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAE 352

Query: 301 ---------------IRQFHCS-STRLLLTPPSPATSFQPVQESG-------STLGQSVE 360
                          +    C+    L + PP P    +  +++              + 
Sbjct: 353 VVGLDRRVEESDIEKLTYLKCTLKETLRMHPPIPLLLHETAEDTSIDGFFIPKKSRVMIN 412

Query: 361 TEPLGKNRT--------------VSGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMA 420
              +G++ T                G   FKGSNFEFIPFGSGRRSCPGMQLGLYA ++A
Sbjct: 413 AFAIGRDPTSWTDPDTFRPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLA 472

Query: 421 VANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRL 426
           VA+++HCF+WKLPDGM  +ELDMND FGLTAP+A RL AVP  RL
Sbjct: 473 VAHILHCFTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVPTTRL 516

BLAST of CcUC02G022300 vs. TAIR 10
Match: AT5G04330.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 406.0 bits (1042), Expect = 3.9e-113
Identity = 228/473 (48.20%), Postives = 301/473 (63.64%), Query Frame = 0

Query: 1   MLMMGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVA 60
           +LMM Q  HRGLA+L+ IYGGL HLRLG  H+ VVS+PD+AR+ LQVQD  F+NR   +A
Sbjct: 45  ILMMNQFNHRGLAKLSRIYGGLLHLRLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIA 104

Query: 61  ITYLTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVRDEVDALVRMVSSKMGQ 120
           I YLTY  +D+AF NYGPFWR+MRK+ V+ LFSR+RAESW SV +EV   VR+V+S +G+
Sbjct: 105 IRYLTYGGSDLAFCNYGPFWRRMRKLYVMMLFSRKRAESWVSVDEEVHKSVRLVASNVGK 164

Query: 121 PVNIGDLVFSLTRNITYKAAFGSRSHEGQ----DELVKILQEFSKLFGAFNIAHLLP-WL 180
           P+NI  L FSL+R+IT++AAFGS S        DE ++I+QEFSKLFG FN+A  +P WL
Sbjct: 165 PLNICKLAFSLSRDITFRAAFGSSSSTSDESRLDEFLEIIQEFSKLFGEFNVADYVPSWL 224

Query: 181 GWIHARDFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLA 240
            WI  +  N R+ KAR+ L  FI ++ID+HL KK+    ++ D+ +E++DMVD+L+AF  
Sbjct: 225 SWIDPQGINGRVEKARKSLDGFIESVIDDHLHKKK----REHDNVDEETDMVDQLLAFYE 284

Query: 241 DESSNNEFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNKKPS 300
           +E   N      S+ K+  DNIK IIMDVMFGGTETV   IEW + E++++PE   +   
Sbjct: 285 EEVKVN-----NSVTKINLDNIKGIIMDVMFGGTETVALAIEWVLTEILRSPENMKRVQD 344

Query: 301 AF----------IRQFHCSSTRLL---------LTPPSPATSFQPVQE---SGSTLGQS- 360
                       +   H      L         L PP P    + V++   SG  + +  
Sbjct: 345 ELTSVVGLDRWRVEDTHLEKLTFLKCILKETLRLHPPFPLLLHETVKDTEISGYFIPKGS 404

Query: 361 ---VETEPLGKN-RTVSGQGGF-------------KGSNFEFIPFGSGRRSCPGMQLGLY 420
              V T  LG++  + S    F             KG+NFEF+PFGSGRRSCPGMQLGLY
Sbjct: 405 RVMVNTYALGRDPNSWSDPESFNPGRFLNPIAPDLKGNNFEFVPFGSGRRSCPGMQLGLY 464

Query: 421 ACEMAVANLVHCFSWKLPDGMNANELDMNDSFGLTAPRAIRLVAVPGFRLNSP 429
           A E+AVA+L+HCF+W LPDGMN  ++D  +  GLT P+AI LVAVP  RL  P
Sbjct: 465 AFELAVAHLLHCFTWSLPDGMNPGDVDTVEGPGLTVPKAIPLVAVPTTRLLCP 508

BLAST of CcUC02G022300 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 209.9 bits (533), Expect = 4.1e-54
Identity = 133/459 (28.98%), Postives = 229/459 (49.89%), Query Frame = 0

Query: 4   MGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVAITY 63
           +G  PHR L  L+  YG L  L LG + ++VVS+ D+AR+ L+  D  FA+R  +     
Sbjct: 48  LGHHPHRSLCSLSHRYGPLMLLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEK 107

Query: 64  LTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVR-DEVDALVRMVSSKMGQPV 123
           L YD  D+AFA YG +WRQ++ +CV++L S +   S+ +VR +E+  ++  +       V
Sbjct: 108 LFYDGRDVAFAPYGEYWRQIKSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSSSLQV 167

Query: 124 NIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIH-AR 183
           N+ +L+ SLT ++  + A G R + G+ +  ++++    L G F++   +PWLGWI    
Sbjct: 168 NVSELLGSLTNDVISRIALG-RKYSGETDSKELMKRLMMLMGEFSVGTYVPWLGWIDWIS 227

Query: 184 DFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSNN 243
             + ++ K   DL  F+  ++ +H+           D + + +D VD L+    ++S   
Sbjct: 228 GLDGQLNKTGNDLDEFLEKVVQDHV-----------DGDGQRTDFVDVLLRIQREKSIGF 287

Query: 244 EFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPE------------- 303
           E D      +L    IKAI++DV+  GT++  ++++WAM EL+++PE             
Sbjct: 288 EID------RLC---IKAIVLDVLVAGTDSSYALMDWAMTELLRHPECLRTLQEEVRTIC 347

Query: 304 -------EPNKKPSAFIRQFHCSSTRLLLTPP--SPATSFQ-------------PVQESG 363
                  E + +  ++++     +TRL    P  +P  S Q              V  + 
Sbjct: 348 KGNLSVSEEDIQNMSYLKAVIKETTRLHPPLPLLAPHESIQDVILGDYHIPAGTQVMINA 407

Query: 364 STLGQSVET-----EPLGKNRTVSGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMAV 421
             +G+   T     E     R +     F+G NFE +PFG+GRR CP +   +   E+A+
Sbjct: 408 WAIGREAATWGPDAEKFRPERHLDSSVDFRGHNFELVPFGAGRRICPAISFAVVLIEVAL 467

BLAST of CcUC02G022300 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 209.5 bits (532), Expect = 5.3e-54
Identity = 134/459 (29.19%), Postives = 223/459 (48.58%), Query Frame = 0

Query: 4   MGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVAITY 63
           +G+  HR L  L+  YG L  L LG + +++VS+ DMA+E L+  D AFANR  +     
Sbjct: 46  LGRHTHRSLCDLSRRYGPLMLLHLGRVPVLIVSSADMAQEILKTHDQAFANRPRSKLSQK 105

Query: 64  LTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVR-DEVDALVRMVSSKMGQPV 123
           L Y+  D+A A YG +WRQM+ +CVI L S +   S+  VR +E+  ++  +      P 
Sbjct: 106 LLYNNRDVASAPYGEYWRQMKSVCVIHLLSNKMVRSFRDVREEEITLMMAKIRKSSSLPF 165

Query: 124 NIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIH-AR 183
           N+  ++  LT ++  + A G R + G+ +  K+    S+L G F+I   +PWL W+   R
Sbjct: 166 NVSKVLECLTNDVICRVALG-RKYGGETDFKKLTDRLSELLGTFSIGSFVPWLAWVDWIR 225

Query: 184 DFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSNN 243
            ++ ++ K  +DL  F   ++ +H          ++ D  + +D++D L+    ++S   
Sbjct: 226 GWDAQLDKMGKDLDDFFEKVVQDH----------EDGDRRDGTDLIDALLRVKREKSPGF 285

Query: 244 EFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNK--------- 303
           E +           +IKAI +DV  GG++T  +++EWAM EL+++P+  N+         
Sbjct: 286 EIE---------RVSIKAITLDVFVGGSDTSFTLLEWAMTELLRHPKSLNRLQEEVRTIC 345

Query: 304 KPSAFIRQFHCSSTRLL---------LTPPSP-----------------ATSFQPVQESG 363
           K  + + +      + L         L PP P                   +   V  + 
Sbjct: 346 KGKSRVSEDDIQGMKYLKAVIKEALRLHPPFPMMAPHESTEDVKLRDYHIPAGTQVMMNA 405

Query: 364 STLGQSVET-----EPLGKNRTVSGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMAV 421
             +G+ V T     E     R +     F+G NFE +PFG+GRR CP +   +   E+ +
Sbjct: 406 WAIGREVATWGPDAEEFKPERHLDTSVDFRGQNFELLPFGAGRRICPAVSFAVVLNEVVL 465

BLAST of CcUC02G022300 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 204.9 bits (520), Expect = 1.3e-52
Identity = 134/459 (29.19%), Postives = 228/459 (49.67%), Query Frame = 0

Query: 4   MGQLPHRGLARLAAIYGGLFHLRLGVLHMVVVSTPDMAREFLQVQDAAFANRAANVAITY 63
           +G+ PHR L  L+  YG L  LR G++ ++VVS+ D+AR+ L+  D  FA+R  +     
Sbjct: 48  LGRHPHRSLCSLSNRYGPLMLLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEK 107

Query: 64  LTYDRADMAFANYGPFWRQMRKICVIKLFSRRRAESWASVR-DEVDALVRMVSSKMGQPV 123
           + Y+  D+A A YG +WRQM+ +CV+ L + +   S+ +VR +E+  ++  +       V
Sbjct: 108 IFYEARDVALAPYGEYWRQMKSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQKSSSLQV 167

Query: 124 NIGDLVFSLTRNITYKAAFGSRSHEGQDELVKILQEFSKLFGAFNIAHLLPWLGWIH-AR 183
           N+ +L+ SLT ++  + A G R +  + +  ++++  +KL G F +   +PWL WI    
Sbjct: 168 NLSELLGSLTNDVISRVALG-RKYSDETDFKELMKRLTKLLGEFCVGTYVPWLAWIDWIS 227

Query: 184 DFNERMAKARRDLAVFINTIIDEHLQKKRKKKGKDEDDEEEDSDMVDELMAFLADESSNN 243
             + ++ K   DL  F+  ++ +H           ED + + +D VD L+    ++S   
Sbjct: 228 GLDGQLKKTGNDLDEFLEKVVQDH-----------EDGDAQRTDFVDVLLRIQREKSVGF 287

Query: 244 EFDDSQSILKLTTDNIKAIIMDVMFGGTETVTSVIEWAMAELMKNPEEPNK--------- 303
           E D           +IKAII+DV+ GGT+T  +++EWAM EL+  PE  N+         
Sbjct: 288 EIDRL---------SIKAIILDVVVGGTDTSYALMEWAMTELLHRPECLNRLQEEVRTIC 347

Query: 304 KPSAFIRQFHCSSTRLL---------LTPPSPA----TSFQPVQ------ESGS------ 363
           K ++ + +        L         L PP P      S Q V+       +G+      
Sbjct: 348 KGNSSVSEDDIKDMNYLKAVIKETMRLHPPLPLMVPHESTQDVRLGDYHIPAGTQVMINA 407

Query: 364 -TLGQSVET-----EPLGKNRTVSGQGGFKGSNFEFIPFGSGRRSCPGMQLGLYACEMAV 421
             +G+   T     E     R ++    F+G NFE IPFG+GRR CP +   +   E+ +
Sbjct: 408 WAIGREAATWGPDAEKFRPERHLNSSVDFRGHNFELIPFGAGRRICPAISFAVILIEVTL 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888732.16.5e-18774.69cytochrome P450 84A1-like [Benincasa hispida][more]
KAA0033772.15.7e-18372.77cytochrome P450 84A1-like [Cucumis melo var. makuwa] >TYK22363.1 cytochrome P450... [more]
XP_008448126.17.5e-18372.77PREDICTED: cytochrome P450 84A1-like [Cucumis melo][more]
XP_004148555.12.1e-17772.19cytochrome P450 84A1 [Cucumis sativus] >KGN43170.1 hypothetical protein Csa_0205... [more]
KAG7022266.11.0e-16065.82Cytochrome P450 84A1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q426001.3e-13454.19Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1[more]
F4JW835.5e-11248.20Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1[more]
A6YIH87.5e-6134.20Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1[more]
O223075.4e-5931.96Cytochrome P450 71D11 (Fragment) OS=Lotus japonicus OX=34305 GN=CYP71D11 PE=2 SV... [more]
A0A0N9HT291.7e-5732.82Desmethyl-deoxy-podophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 G... [more]
Match NameE-valueIdentityDescription
A0A5A7SRE02.8e-18372.77Cytochrome P450 84A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3BID83.6e-18372.77cytochrome P450 84A1-like OS=Cucumis melo OX=3656 GN=LOC103490415 PE=3 SV=1[more]
A0A0A0K0F01.0e-17772.19Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G004720 PE=3 SV=1[more]
A0A6J1I1976.6e-16166.17cytochrome P450 84A1-like OS=Cucurbita maxima OX=3661 GN=LOC111469596 PE=3 SV=1[more]
A0A6J1F4G76.6e-16165.96cytochrome P450 84A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440398 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT4G36220.19.5e-13654.19ferulic acid 5-hydroxylase 1 [more]
AT5G04330.13.9e-11348.20Cytochrome P450 superfamily protein [more]
AT3G48320.14.1e-5428.98cytochrome P450, family 71, subfamily A, polypeptide 21 [more]
AT3G48280.15.3e-5429.19cytochrome P450, family 71, subfamily A, polypeptide 25 [more]
AT3G48310.11.3e-5229.19cytochrome P450, family 71, subfamily A, polypeptide 22 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 16..35
score: 33.76
coord: 40..61
score: 24.57
coord: 357..367
score: 57.93
coord: 367..390
score: 23.31
coord: 256..273
score: 30.97
coord: 276..302
score: 21.53
coord: 131..149
score: 20.84
IPR001128Cytochrome P450PRINTSPR00385P450coord: 267..284
score: 30.9
coord: 358..367
score: 58.92
IPR001128Cytochrome P450PFAMPF00067p450coord: 349..412
e-value: 1.4E-9
score: 37.2
coord: 6..288
e-value: 1.2E-37
score: 129.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 332..419
e-value: 2.4E-14
score: 55.2
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..292
e-value: 1.7E-53
score: 183.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 349..414
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 3..289
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 313..345
NoneNo IPR availablePANTHERPTHR47945CYTOCHROME P450 84A1-RELATEDcoord: 1..292
coord: 348..429
NoneNo IPR availablePANTHERPTHR47945:SF6CYTOCHROME P450 FAMILY MONOOXYGENASEcoord: 1..292
coord: 348..429
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 360..369

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC02G022300.1CcUC02G022300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009809 lignin biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen