Carg28295 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28295
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	E3 ubiquitin ligase BIG BROTHER-related
Location	Carg_Chr04: 18490475 .. 18491113 (-)
RNA-Seq Expression	Carg28295
Synteny	Carg28295

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAGCAAGAGGATAAGCAACTCCCTCAGAGGATTTCCCGTGCTTATGTGGAACAGGTGAACACGGATTTTATTATGGCCTTGGCAATGCAGCAGCAGGAGCATGAGAGGAACTACACGATGCTCGAGACCATCGAAAGTGATAGTGAAGAAGATGAACGTTCAGATTCCAACAGCAGCAATGGTCTCGACCACACCAATGACTCCTTCCAAAGCCAAGAACTTCATTCCCCGTGGGGTTTTCTTGCAGACGAGGAGGAGGAGGAGACTACGGATGATAATGAATATATGGACGAGGACGCAGAAGAAGATATCGAGGAGTTTGATTTGGATGAGCTAAGCTATGAAGAGTTGATTGCATTAGGGGAGTTCATTGGAGAAGAAAAGAGGGGACTACCGATGAACGAAATACCTTCGTGCTTGCACTCGAGCAAGTTTCAAACCATCGAAAACAAAAGCGGTATAGATCGGTGCGTGATTTGCCAAGTTGAATATGAAGATAGTGAAGAACTGGCTGCCCTTCCTTGTGAGCATCCTTACCATTCAGAATGTATAAGCAAATGGCTTCAAATCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAAGCCTTCTAAAAATGCTTAA

mRNA sequence

ATGGAAGAGCAAGAGGATAAGCAACTCCCTCAGAGGATTTCCCGTGCTTATGTGGAACAGGTGAACACGGATTTTATTATGGCCTTGGCAATGCAGCAGCAGGAGCATGAGAGGAACTACACGATGCTCGAGACCATCGAAAGTGATAGTGAAGAAGATGAACGTTCAGATTCCAACAGCAGCAATGGTCTCGACCACACCAATGACTCCTTCCAAAGCCAAGAACTTCATTCCCCGTGGGGTTTTCTTGCAGACGAGGAGGAGGAGGAGACTACGGATGATAATGAATATATGGACGAGGACGCAGAAGAAGATATCGAGGAGTTTGATTTGGATGAGCTAAGCTATGAAGAGTTGATTGCATTAGGGGAGTTCATTGGAGAAGAAAAGAGGGGACTACCGATGAACGAAATACCTTCGTGCTTGCACTCGAGCAAGTTTCAAACCATCGAAAACAAAAGCGGTATAGATCGGTGCGTGATTTGCCAAGTTGAATATGAAGATAGTGAAGAACTGGCTGCCCTTCCTTGTGAGCATCCTTACCATTCAGAATGTATAAGCAAATGGCTTCAAATCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAAGCCTTCTAAAAATGCTTAA

Coding sequence (CDS)

ATGGAAGAGCAAGAGGATAAGCAACTCCCTCAGAGGATTTCCCGTGCTTATGTGGAACAGGTGAACACGGATTTTATTATGGCCTTGGCAATGCAGCAGCAGGAGCATGAGAGGAACTACACGATGCTCGAGACCATCGAAAGTGATAGTGAAGAAGATGAACGTTCAGATTCCAACAGCAGCAATGGTCTCGACCACACCAATGACTCCTTCCAAAGCCAAGAACTTCATTCCCCGTGGGGTTTTCTTGCAGACGAGGAGGAGGAGGAGACTACGGATGATAATGAATATATGGACGAGGACGCAGAAGAAGATATCGAGGAGTTTGATTTGGATGAGCTAAGCTATGAAGAGTTGATTGCATTAGGGGAGTTCATTGGAGAAGAAAAGAGGGGACTACCGATGAACGAAATACCTTCGTGCTTGCACTCGAGCAAGTTTCAAACCATCGAAAACAAAAGCGGTATAGATCGGTGCGTGATTTGCCAAGTTGAATATGAAGATAGTGAAGAACTGGCTGCCCTTCCTTGTGAGCATCCTTACCATTCAGAATGTATAAGCAAATGGCTTCAAATCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAAGCCTTCTAAAAATGCTTAA

Protein sequence

MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNSSNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHPYHSECISKWLQIKRVCPICGTEVSSPKPSKNA

Homology

[Close]

BLAST of Carg28295 vs. NCBI nr
Match: KAG7032814.1 (E3 ubiquitin ligase BIG BROTHER-related, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 415.2 bits (1066), Expect = 3.3e-112
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60
           MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  SNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEELI 120
           SNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEELI
Sbjct: 61  SNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180
           ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 213
           YHSECISKWLQIKRVCPICGTEVSSPKPSKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 212

[Close]

BLAST of Carg28295 vs. NCBI nr
Match: XP_022954880.1 (E3 ubiquitin ligase BIG BROTHER-related [Cucurbita moschata])

HSP 1 Score: 406.4 bits (1043), Expect = 1.5e-109
Identity = 209/212 (98.58%), Postives = 210/212 (99.06%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60
           MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  SNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEELI 120
           SNGLDHTNDSFQSQELHSPWGFLAD +EEETTDDNEYMDED EEDIEEFDLDELSYEELI
Sbjct: 61  SNGLDHTNDSFQSQELHSPWGFLAD-DEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180
           ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 213
           YHSECISKWLQIKRVCPICGTEVSSPKPSKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 211

[Close]

BLAST of Carg28295 vs. NCBI nr
Match: XP_023523684.1 (E3 ubiquitin ligase BIG BROTHER-related-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 398.3 bits (1022), Expect = 4.2e-107
Identity = 207/213 (97.18%), Postives = 209/213 (98.12%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60
           MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  SNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDED-AEEDIEEFDLDELSYEEL 120
           SNGLDHTNDSFQSQELHSPWGFLAD +EEETTDDNEYMDED  EEDIEEFDLDELSYEEL
Sbjct: 61  SNGLDHTNDSFQSQELHSPWGFLAD-DEEETTDDNEYMDEDEEEEDIEEFDLDELSYEEL 120

Query: 121 IALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEH 180
           IALGEFIGEEKRGLP+NEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEH
Sbjct: 121 IALGEFIGEEKRGLPINEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEH 180

Query: 181 PYHSECISKWLQIKRVCPICGTEVSSPKPSKNA 213
           PYHSECIS WLQIKRVCPICGTEVSSPKPSKNA
Sbjct: 181 PYHSECISNWLQIKRVCPICGTEVSSPKPSKNA 212

[Close]

BLAST of Carg28295 vs. NCBI nr
Match: XP_022990800.1 (E3 ubiquitin ligase BIG BROTHER-related-like [Cucurbita maxima])

HSP 1 Score: 397.9 bits (1021), Expect = 5.5e-107
Identity = 206/212 (97.17%), Postives = 207/212 (97.64%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60
           MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  SNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEELI 120
           SNGLDHTNDS QSQELHSPWGFLAD +EEETTDDNEYMDED EEDIEEFDLDELSYEELI
Sbjct: 61  SNGLDHTNDSLQSQELHSPWGFLAD-DEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180
           ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 213
           YHSECISKWLQIKRVCPICGTEVSS K SKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSSKSSKNA 211

[Close]

BLAST of Carg28295 vs. NCBI nr
Match: KAG6602111.1 (Proteasome subunit beta type-3-A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 370.2 bits (949), Expect = 1.2e-98
Identity = 193/209 (92.34%), Postives = 197/209 (94.26%), Query Frame = 0


Query: 6   DKQLPQR--ISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNSSNG 65
           DK   QR  +   +  +VNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNSSNG
Sbjct: 223 DKGCQQREAVVEPWRGKVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNSSNG 282

Query: 66  LDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEELIALG 125
           LDHTNDSFQSQELHSPWGFLAD +EEETTDDNEYMDED EEDIEEFDLDELSYEELIALG
Sbjct: 283 LDHTNDSFQSQELHSPWGFLAD-DEEETTDDNEYMDEDEEEDIEEFDLDELSYEELIALG 342

Query: 126 EFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHPYHS 185
           EFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHPYHS
Sbjct: 343 EFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHPYHS 402

Query: 186 ECISKWLQIKRVCPICGTEVSSPKPSKNA 213
           ECISKWLQIKRVCPICGTEVSSPKPSKNA
Sbjct: 403 ECISKWLQIKRVCPICGTEVSSPKPSKNA 430

[Close]

BLAST of Carg28295 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-22
Identity = 88/259 (33.98%), Postives = 123/259 (47.49%), Query Frame = 0


Query: 12  RISRAYVEQVNTDFIMALAMQQQEHERNYTML---------------------------- 71
           R+    + Q++ D  +ALA   QE ER Y ML                            
Sbjct: 85  RVPFTNLSQIDAD--LALARTLQEQERAYMMLTMNSEISDYGSWETGSYVYDEDEFDDPE 144

Query: 72  -ETIESDSEEDERSDSNSSNGLD---HTNDSFQSQELHSPWGFLADEEEEETTDDNEY-- 131
            E  + D +E E  D    +GLD   H N+  Q  + +S      D EE   TDD  Y  
Sbjct: 145 NEDEDDDEDEYETDDDPQEDGLDVNVHANEDDQEDDGNS------DIEEVAYTDDEAYAR 204

Query: 132 -MDEDAEEDI-----------------------------EEFDLDELSYEELIALGEFIG 191
            + E  E D+                             +E D DELSYEEL+ALG+ +G
Sbjct: 205 ALQEAEERDMAARLSALSGLANRVVEDLEDESHTSQDAWDEMDPDELSYEELLALGDIVG 264

Query: 192 EEKRGLPMNEIPSCLHSSKFQTIENKSGI-DRCVICQVEYEDSEELAALPCEHPYHSECI 206
            E RGL  + I S L S +++  +N++G  + CVIC+++YED E+L  LPC+H YHSECI
Sbjct: 265 TESRGLSADTIAS-LPSKRYKEGDNQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECI 324

[Close]

BLAST of Carg28295 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 8.4e-18
Identity = 51/120 (42.50%), Postives = 70/120 (58.33%), Query Frame = 0


Query: 87  EEEETTDDNEYMDEDAEEDIEEFDLDELSYEELIALGEFIGEEKRGLPMNEIPSCLHSSK 146
           E EE   +    D       ++ D D ++YEEL+ LGE +G E RGL   E+   L + K
Sbjct: 123 EAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGL-SQELIETLPTKK 182

Query: 147 FQ---TIENKSGIDRCVICQVEYEDSEELAALPCEHPYHSECISKWLQIKRVCPICGTEV 204
           ++       K   +RCVICQ++Y+  E    LPC+H YHSECISKWL I +VCP+C +EV
Sbjct: 183 YKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEV 241

[Close]

BLAST of Carg28295 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 42/98 (42.86%), Postives = 57/98 (58.16%), Query Frame = 0


Query: 110 DLDELSYEELIALGEFIGEEKRGLPMNEIPSCLH-----SSKFQTIENKSGIDRCVICQV 169
           D+D +SYEEL+ALGE IG+   GL    I   +      SS   ++E    I+ C ICQ 
Sbjct: 602 DVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQE 661

Query: 170 EYEDSEELAALPCEHPYHSECISKWLQIKRVCPICGTE 203
           EY + + L  L C H +H +CI +W+ IK +CPIC TE
Sbjct: 662 EYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]

BLAST of Carg28295 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 44/105 (41.90%), Postives = 59/105 (56.19%), Query Frame = 0


Query: 110 DLDELSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKF----QTIENKSG-----IDRCV 169
           D+D ++YEEL+AL E IG+   GL  + I   L +S F      + NKS       D C+
Sbjct: 413 DIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLKTSLFVPGSSCMSNKSSESSMENDACI 472

Query: 170 ICQVEYEDSEELAALPCEHPYHSECISKWLQIKRVCPICGTEVSS 206
           ICQ EY+  E +  L C H YH +CI +WL +K +CPIC T   S
Sbjct: 473 ICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKNLCPICKTTALS 517

[Close]

BLAST of Carg28295 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 6.7e-15
Identity = 36/94 (38.30%), Postives = 54/94 (57.45%), Query Frame = 0


Query: 110 DLDELSYEELIALGEFIGEEKRGLPMNEIPSCL--HSSKFQTIENKSGIDRCVICQVEYE 169
           D+D +SYEEL+ALGE IG+   GL    I   +  H        +   ++ C +CQ EY 
Sbjct: 568 DVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCVCQEEYA 627

Query: 170 DSEELAALPCEHPYHSECISKWLQIKRVCPICGT 202
           + ++L  L C H +H+ C+ +WL +K +CPIC T
Sbjct: 628 EGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKT 661

[Close]

BLAST of Carg28295 vs. ExPASy TrEMBL
Match: A0A6J1GTM7 (E3 ubiquitin ligase BIG BROTHER-related OS=Cucurbita moschata OX=3662 GN=LOC111457005 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 7.5e-110
Identity = 209/212 (98.58%), Postives = 210/212 (99.06%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60
           MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  SNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEELI 120
           SNGLDHTNDSFQSQELHSPWGFLAD +EEETTDDNEYMDED EEDIEEFDLDELSYEELI
Sbjct: 61  SNGLDHTNDSFQSQELHSPWGFLAD-DEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180
           ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 213
           YHSECISKWLQIKRVCPICGTEVSSPKPSKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 211

[Close]

BLAST of Carg28295 vs. ExPASy TrEMBL
Match: A0A6J1JR32 (E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC111487582 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 2.7e-107
Identity = 206/212 (97.17%), Postives = 207/212 (97.64%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60
           MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  SNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEELI 120
           SNGLDHTNDS QSQELHSPWGFLAD +EEETTDDNEYMDED EEDIEEFDLDELSYEELI
Sbjct: 61  SNGLDHTNDSLQSQELHSPWGFLAD-DEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180
           ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 213
           YHSECISKWLQIKRVCPICGTEVSS K SKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSSKSSKNA 211

[Close]

BLAST of Carg28295 vs. ExPASy TrEMBL
Match: A0A6J1CJH0 (E3 ubiquitin ligase BIG BROTHER-related OS=Momordica charantia OX=3673 GN=LOC111011883 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.7e-85
Identity = 173/208 (83.17%), Postives = 187/208 (89.90%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60
           MEEQEDKQ PQRIS AYVEQVN+DFIMALAMQQQEHER YTMLETIESDS+EDE  DSNS
Sbjct: 1   MEEQEDKQPPQRISLAYVEQVNSDFIMALAMQQQEHERAYTMLETIESDSDEDESFDSNS 60

Query: 61  SNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEED-IEEFDLDELSYEEL 120
           + GLD T D  QSQE HS W FLAD  +EETTD++  MDED +ED +++FDLDEL+YEEL
Sbjct: 61  NTGLD-TYDPLQSQEFHSRWDFLAD--DEETTDED--MDEDVDEDELDDFDLDELTYEEL 120

Query: 121 IALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEH 180
           +ALGEFIGEEKRGLPMNEIPSCLHS KF+TIENKSG+DRCVICQVEYED EELAALPCEH
Sbjct: 121 VALGEFIGEEKRGLPMNEIPSCLHSGKFRTIENKSGMDRCVICQVEYEDGEELAALPCEH 180

Query: 181 PYHSECISKWLQIKRVCPICGTEVSSPK 208
           PYHSECISKWLQIKRVCPICGTEVSSPK
Sbjct: 181 PYHSECISKWLQIKRVCPICGTEVSSPK 203

[Close]

BLAST of Carg28295 vs. ExPASy TrEMBL
Match: A0A0A0KFS4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382920 PE=4 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 3.9e-82
Identity = 172/219 (78.54%), Postives = 184/219 (84.02%), Query Frame = 0


Query: 1   MEEQEDK--------QLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEE 60
           MEEQED         QLPQRIS AYVEQVNTD IMALAMQQQEHE  YT LETI SDSEE
Sbjct: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHELAYTTLETIASDSEE 60

Query: 61  DERSDSNSSNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLD 120
           DE SDSNS+NGLD TN + Q QEL S W FL  E++EETTD N+ M+ED + D E FDLD
Sbjct: 61  DENSDSNSNNGLD-TNATSQRQELVSRWAFL--EDDEETTDGNDDMEEDEDGDFEGFDLD 120

Query: 121 ELSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEEL 180
           EL+YEELIALGEFIGEEKRGLP+NEIPSCLHSSKFQTIEN+SGIDRCVICQVEY+D EEL
Sbjct: 121 ELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEEL 180

Query: 181 AALPCEHPYHSECISKWLQIKRVCPICGTEVSSPKPSKN 212
           AALPCEHPYHSECI +WLQIKRVCPICGTEVSSP  SKN
Sbjct: 181 AALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKN 216

[Close]

BLAST of Carg28295 vs. ExPASy TrEMBL
Match: A0A6J1K3Q9 (E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC111489783 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.4e-79
Identity = 170/213 (79.81%), Postives = 180/213 (84.51%), Query Frame = 0


Query: 1   MEEQEDK-QLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSN 60
           M EQEDK QLPQRIS  YVEQVN+D IMALAMQQQEHE  YT L TIESDS+EDERSDSN
Sbjct: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60

Query: 61  SSNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEEL 120
           S+NGLD TN S QSQ       FLAD   EETTD+NE MDED EE  E+FD DEL+YEEL
Sbjct: 61  SNNGLD-TNASLQSQ-------FLAD--NEETTDENEDMDEDDEEGFEDFDFDELTYEEL 120

Query: 121 IALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEH 180
           IALGEFIGEEKRGLP NEIPSCLHSSKF++IEN SGIDRCVICQVEYED EELAALPCEH
Sbjct: 121 IALGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEH 180

Query: 181 PYHSECISKWLQIKRVCPICGTEVSSPKPSKNA 213
           PYHS+CI KW+Q KRVCPICGTEVSSPK SKNA
Sbjct: 181 PYHSDCIVKWVQTKRVCPICGTEVSSPKASKNA 203

[Close]

BLAST of Carg28295 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-32
Identity = 87/212 (41.04%), Postives = 128/212 (60.38%), Query Frame = 0


Query: 2   EEQEDKQLPQRI-SRAYVEQVNTDFIMALAMQQQE-----HERNYTMLETIESD--SEED 61
           +E+E KQ P ++   A  EQ N++  +A +          H+ + +M+  IESD  SE++
Sbjct: 4   QEEEPKQPPNKLPDLALFEQANSEVALAASQANSHFAHAMHDSSPSMISMIESDEESEDE 63

Query: 62  ERSDSNSSNGLDHTNDSFQSQELHSPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDE 121
           E  + N     D         E++    FL D+E     ++    ++D  E+ +E D D+
Sbjct: 64  EEINENYYEYFDSNGFGVDEDEINE---FLEDQESNSNLEE----EDDFLEEEDEIDPDQ 123

Query: 122 LSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELA 181
           LSYEELIALG+FIG E RGL   EI +CL++S +    NK+ IDRCV+CQ+E+E+ E L 
Sbjct: 124 LSYEELIALGDFIGVENRGLTPIEISTCLNASTYVFSHNKNEIDRCVVCQMEFEERESLV 183

Query: 182 AL-PCEHPYHSECISKWLQIKRVCPICGTEVS 205
            L PC+HPYHSECI+KWL+ K++CPIC +E S
Sbjct: 184 VLRPCDHPYHSECITKWLETKKICPICCSEPS 208

[Close]

BLAST of Carg28295 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.4e-23
Identity = 88/259 (33.98%), Postives = 123/259 (47.49%), Query Frame = 0


Query: 12  RISRAYVEQVNTDFIMALAMQQQEHERNYTML---------------------------- 71
           R+    + Q++ D  +ALA   QE ER Y ML                            
Sbjct: 85  RVPFTNLSQIDAD--LALARTLQEQERAYMMLTMNSEISDYGSWETGSYVYDEDEFDDPE 144

Query: 72  -ETIESDSEEDERSDSNSSNGLD---HTNDSFQSQELHSPWGFLADEEEEETTDDNEY-- 131
            E  + D +E E  D    +GLD   H N+  Q  + +S      D EE   TDD  Y  
Sbjct: 145 NEDEDDDEDEYETDDDPQEDGLDVNVHANEDDQEDDGNS------DIEEVAYTDDEAYAR 204

Query: 132 -MDEDAEEDI-----------------------------EEFDLDELSYEELIALGEFIG 191
            + E  E D+                             +E D DELSYEEL+ALG+ +G
Sbjct: 205 ALQEAEERDMAARLSALSGLANRVVEDLEDESHTSQDAWDEMDPDELSYEELLALGDIVG 264

Query: 192 EEKRGLPMNEIPSCLHSSKFQTIENKSGI-DRCVICQVEYEDSEELAALPCEHPYHSECI 206
            E RGL  + I S L S +++  +N++G  + CVIC+++YED E+L  LPC+H YHSECI
Sbjct: 265 TESRGLSADTIAS-LPSKRYKEGDNQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECI 324

[Close]

BLAST of Carg28295 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 6.0e-19
Identity = 51/120 (42.50%), Postives = 70/120 (58.33%), Query Frame = 0


Query: 87  EEEETTDDNEYMDEDAEEDIEEFDLDELSYEELIALGEFIGEEKRGLPMNEIPSCLHSSK 146
           E EE   +    D       ++ D D ++YEEL+ LGE +G E RGL   E+   L + K
Sbjct: 123 EAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGL-SQELIETLPTKK 182

Query: 147 FQ---TIENKSGIDRCVICQVEYEDSEELAALPCEHPYHSECISKWLQIKRVCPICGTEV 204
           ++       K   +RCVICQ++Y+  E    LPC+H YHSECISKWL I +VCP+C +EV
Sbjct: 183 YKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEV 241

[Close]

BLAST of Carg28295 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 6.0e-19
Identity = 51/120 (42.50%), Postives = 70/120 (58.33%), Query Frame = 0


Query: 87  EEEETTDDNEYMDEDAEEDIEEFDLDELSYEELIALGEFIGEEKRGLPMNEIPSCLHSSK 146
           E EE   +    D       ++ D D ++YEEL+ LGE +G E RGL   E+   L + K
Sbjct: 123 EAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGL-SQELIETLPTKK 182

Query: 147 FQ---TIENKSGIDRCVICQVEYEDSEELAALPCEHPYHSECISKWLQIKRVCPICGTEV 204
           ++       K   +RCVICQ++Y+  E    LPC+H YHSECISKWL I +VCP+C +EV
Sbjct: 183 YKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEV 241

[Close]

BLAST of Carg28295 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 8.7e-18
Identity = 42/98 (42.86%), Postives = 57/98 (58.16%), Query Frame = 0


Query: 110 DLDELSYEELIALGEFIGEEKRGLPMNEIPSCLH-----SSKFQTIENKSGIDRCVICQV 169
           D+D +SYEEL+ALGE IG+   GL    I   +      SS   ++E    I+ C ICQ 
Sbjct: 602 DVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQE 661

Query: 170 EYEDSEELAALPCEHPYHSECISKWLQIKRVCPICGTE 203
           EY + + L  L C H +H +CI +W+ IK +CPIC TE
Sbjct: 662 EYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

The following BLAST results are available for this feature:

BLAST of Carg28295 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032814.1	3.3e-112	100.00	E3 ubiquitin ligase BIG BROTHER-related, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022954880.1	1.5e-109	98.58	E3 ubiquitin ligase BIG BROTHER-related [Cucurbita moschata]	[more]
XP_023523684.1	4.2e-107	97.18	E3 ubiquitin ligase BIG BROTHER-related-like isoform X1 [Cucurbita pepo subsp. p...	[more]
XP_022990800.1	5.5e-107	97.17	E3 ubiquitin ligase BIG BROTHER-related-like [Cucurbita maxima]	[more]
KAG6602111.1	1.2e-98	92.34	Proteasome subunit beta type-3-A, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of Carg28295 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LT17	1.9e-22	33.98	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
Q8L649	8.4e-18	42.50	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9ZQF9	1.2e-16	42.86	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]
Q5QLR5	1.3e-15	41.90	Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399...	[more]
O49500	6.7e-15	38.30	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]

BLAST of Carg28295 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GTM7	7.5e-110	98.58	E3 ubiquitin ligase BIG BROTHER-related OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1JR32	2.7e-107	97.17	E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1CJH0	3.7e-85	83.17	E3 ubiquitin ligase BIG BROTHER-related OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A0A0KFS4	3.9e-82	78.54	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382920 P...	[more]
A0A6J1K3Q9	1.4e-79	79.81	E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of Carg28295 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47180.1	1.2e-32	41.04	RING/U-box superfamily protein	[more]
AT3G19910.1	1.4e-23	33.98	RING/U-box superfamily protein	[more]
AT3G63530.1	6.0e-19	42.50	RING/U-box superfamily protein	[more]
AT3G63530.2	6.0e-19	42.50	RING/U-box superfamily protein	[more]
AT2G15530.1	8.7e-18	42.86	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 159..199 e-value: 1.6E-5 score: 34.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 158..199 e-value: 1.8E-11 score: 44.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 159..199 score: 11.66311
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 136..208 e-value: 3.0E-17 score: 63.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..102
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..102
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..77
None	No IPR available	PANTHER	PTHR46400:SF8	E3 UBIQUITIN LIGASE BIG BROTHER-RELATED-LIKE	coord: 1..208
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 158..206
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28295-RA	Carg28295-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0019774	proteasome core complex, beta-subunit complex
molecular_function	GO:0004298	threonine-type endopeptidase activity
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity