Carg28176 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28176
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Carg_Chr10: 3885172 .. 3885777 (-)
RNA-Seq Expression	Carg28176
Synteny	Carg28176

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTCCCTTTCTCTCCTCCTCGTCCTCCTCCTCTCTCTCTCCCTCTCCGCCGCAGCCCACGATGGCCCTCCGCCGTCCCAAGATCTGATCCACTCCTCCTGCCTTCAAGCCAGCTACCCAAGTCTCTGCATCAGAACCCTCTCCTCCTACTCTGGCGCCGTCAACACCCCACAAGATCTCGCCCAAGCCACCCTCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCAATTCATCCGCCAACCTCCGGCAGGCCTCGAAGCGGCAACGCGCGGCGGTTGACGACTGCGTGGAACAGATTGGAGACTCTGTCGAGGAGCTGAGCAACACTCTGGGTCTCCTCCGCCACCTCCCCTACGGCGACCGCCGGTCTTTCCGATTGCAAATGAGCAATGCCCAGACGTGGGTCAGCGCCGCCTTGACCAATGAAGACACGTGTCTCGATGGGTTTAAGGAAGTGGATGGGGAGGTGAAATTTGATGTTAAGAAGAGGATTTCTAAGGTCGCTAAAGCTACTAGTAATGCTCTGTATATGATTAATCGCCTCGACGTCGGAACCCCCGCCGGGAGGAAGAAATTGACCGCTCCTTGA

mRNA sequence

ATGACTTCCCTTTCTCTCCTCCTCGTCCTCCTCCTCTCTCTCTCCCTCTCCGCCGCAGCCCACGATGGCCCTCCGCCGTCCCAAGATCTGATCCACTCCTCCTGCCTTCAAGCCAGCTACCCAAGTCTCTGCATCAGAACCCTCTCCTCCTACTCTGGCGCCGTCAACACCCCACAAGATCTCGCCCAAGCCACCCTCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCAATTCATCCGCCAACCTCCGGCAGGCCTCGAAGCGGCAACGCGCGGCGGTTGACGACTGCGTGGAACAGATTGGAGACTCTGTCGAGGAGCTGAGCAACACTCTGGGTCTCCTCCGCCACCTCCCCTACGGCGACCGCCGGTCTTTCCGATTGCAAATGAGCAATGCCCAGACGTGGGTCAGCGCCGCCTTGACCAATGAAGACACGTGTCTCGATGGGTTTAAGGAAGTGGATGGGGAGGTGAAATTTGATGTTAAGAAGAGGATTTCTAAGGTCGCTAAAGCTACTAGTAATGCTCTGTATATGATTAATCGCCTCGACGTCGGAACCCCCGCCGGGAGGAAGAAATTGACCGCTCCTTGA

Coding sequence (CDS)

ATGACTTCCCTTTCTCTCCTCCTCGTCCTCCTCCTCTCTCTCTCCCTCTCCGCCGCAGCCCACGATGGCCCTCCGCCGTCCCAAGATCTGATCCACTCCTCCTGCCTTCAAGCCAGCTACCCAAGTCTCTGCATCAGAACCCTCTCCTCCTACTCTGGCGCCGTCAACACCCCACAAGATCTCGCCCAAGCCACCCTCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCAATTCATCCGCCAACCTCCGGCAGGCCTCGAAGCGGCAACGCGCGGCGGTTGACGACTGCGTGGAACAGATTGGAGACTCTGTCGAGGAGCTGAGCAACACTCTGGGTCTCCTCCGCCACCTCCCCTACGGCGACCGCCGGTCTTTCCGATTGCAAATGAGCAATGCCCAGACGTGGGTCAGCGCCGCCTTGACCAATGAAGACACGTGTCTCGATGGGTTTAAGGAAGTGGATGGGGAGGTGAAATTTGATGTTAAGAAGAGGATTTCTAAGGTCGCTAAAGCTACTAGTAATGCTCTGTATATGATTAATCGCCTCGACGTCGGAACCCCCGCCGGGAGGAAGAAATTGACCGCTCCTTGA

Protein sequence

MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALYMINRLDVGTPAGRKKLTAP

Homology

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BLAST of Carg28176 vs. NCBI nr
Match: XP_022961187.1 (pectinesterase inhibitor 3 [Cucurbita moschata] >KAG6590055.1 Pectinesterase inhibitor 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023750.1 Pectinesterase inhibitor 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 381.3 bits (978), Expect = 5.0e-102
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD
Sbjct: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180
           HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA
Sbjct: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180

Query: 181 LYMINRLDVGTPAGRKKLTAP 202
           LYMINRLDVGTPAGRKKLTAP
Sbjct: 181 LYMINRLDVGTPAGRKKLTAP 201

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BLAST of Carg28176 vs. NCBI nr
Match: XP_023516503.1 (pectinesterase inhibitor 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 377.5 bits (968), Expect = 7.3e-101
Identity = 199/201 (99.00%), Postives = 199/201 (99.00%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MTSLSLLL LLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD
Sbjct: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180
           HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA
Sbjct: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180

Query: 181 LYMINRLDVGTPAGRKKLTAP 202
           LYMINRLDVGTPAGRKKL AP
Sbjct: 181 LYMINRLDVGTPAGRKKLAAP 201

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BLAST of Carg28176 vs. NCBI nr
Match: XP_022987422.1 (pectinesterase inhibitor 3 [Cucurbita maxima])

HSP 1 Score: 372.5 bits (955), Expect = 2.3e-99
Identity = 196/201 (97.51%), Postives = 197/201 (98.01%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MTSLSLLL LLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD
Sbjct: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQATLSVSLSLARDLSRYLSNSS NLRQASKRQR AVDDCVEQIGDSVEELSNTLGLLR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSVNLRQASKRQRVAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180
           HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA
Sbjct: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180

Query: 181 LYMINRLDVGTPAGRKKLTAP 202
           LYMINRL+VGTPAGRKKL AP
Sbjct: 181 LYMINRLNVGTPAGRKKLAAP 201

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BLAST of Carg28176 vs. NCBI nr
Match: XP_038879670.1 (pectinesterase inhibitor 3 [Benincasa hispida])

HSP 1 Score: 297.7 bits (761), Expect = 7.3e-77
Identity = 165/202 (81.68%), Postives = 177/202 (87.62%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT LSLLL+L LSLS   AAHDG   SQDLIHSSCLQA YP+LCIRTLSSY+GAV TPQ+
Sbjct: 33  MTFLSLLLLLSLSLS---AAHDGAAVSQDLIHSSCLQARYPNLCIRTLSSYAGAVTTPQE 92

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQA +SVSLSLARDLSRYLS++   LRQAS RQRAAV+DCV+QIGDSVEELSNTLG+LR
Sbjct: 93  LAQAAISVSLSLARDLSRYLSDA---LRQASMRQRAAVNDCVDQIGDSVEELSNTLGVLR 152

Query: 121 HLPY-GDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           HL   GDRR FRLQM NA+TWVSAALTNEDTCLDGFKEVDGEVK DVKKRISKVAK TSN
Sbjct: 153 HLTCGGDRRDFRLQMGNAKTWVSAALTNEDTCLDGFKEVDGEVKLDVKKRISKVAKVTSN 212

Query: 181 ALYMINRLDVGTPAGRKKLTAP 202
           AL+MINRLD G PAGRKKL AP
Sbjct: 213 ALFMINRLDGGNPAGRKKLAAP 228

[Close]

BLAST of Carg28176 vs. NCBI nr
Match: XP_004144132.2 (pectinesterase inhibitor 3 [Cucumis sativus] >KGN66330.1 hypothetical protein Csa_007271 [Cucumis sativus])

HSP 1 Score: 280.4 bits (716), Expect = 1.2e-71
Identity = 157/199 (78.89%), Postives = 173/199 (86.93%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT LSLLL LL SLSLSAA       SQDLIHSSCLQASYP+LCIRTLSSY+GAV TP+D
Sbjct: 28  MTVLSLLL-LLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 87

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQAT+SVSLSLA++LS YLS+S   LRQAS++QRAAVDDCV+QIGDSVEELSNTLG+LR
Sbjct: 88  LAQATISVSLSLAQNLSEYLSDS---LRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLR 147

Query: 121 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           HLP G DRR FRL+M NA+TWVSAALTNE+TCLDGFKEVDGEVK DVK+RI KVAK TSN
Sbjct: 148 HLPCGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSN 207

Query: 181 ALYMINRLDVGTPAGRKKL 199
           AL+MINRLD G P GRK L
Sbjct: 208 ALFMINRLDGGNPTGRKML 222

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BLAST of Carg28176 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.7e-42
Identity = 96/190 (50.53%), Postives = 138/190 (72.63%), Query Frame = 0


Query: 3   SLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSG-AVNTPQDL 62
           +L L+ +    +  ++  H     ++D++HSSC  ASYPSLC+RTLSSYSG  +   +DL
Sbjct: 6   NLFLVAIAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDL 65

Query: 63  AQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRH 122
           AQA + +SLS A+  ++ L+    ++ +  K+++AA+ DCVE IGDSV+ELS TLG+L+H
Sbjct: 66  AQAAIKISLSHAQSAAKKLAVVRDSVGK-KKQEKAALVDCVEMIGDSVDELSRTLGVLKH 125

Query: 123 LPY--GDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEV-DGEVKFDVKKRISKVAKATS 182
           L    G  + FR QMSNAQTW SAALT++DTCLDGF+ + DGE+K +VK+ ++KVA+ TS
Sbjct: 126 LRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTS 185

Query: 183 NALYMINRLD 189
           NALYM+N+LD
Sbjct: 186 NALYMVNQLD 194

[Close]

BLAST of Carg28176 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.2e-26
Identity = 73/189 (38.62%), Postives = 118/189 (62.43%), Query Frame = 0


Query: 5   SLLLVLLLSLSL--SAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAV--NTPQD 64
           ++ LV+LLS+++  S++A      S   I SSC    YPSLC+ TLS+Y+  +  N  QD
Sbjct: 6   TIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQD 65

Query: 65  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 124
           LAQ  L +SL+ A+ ++ +++  +    +  +R+  A+ DC+E +G+SV+ L+ ++  L 
Sbjct: 66  LAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELA 125

Query: 125 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKE--VDGEVKFDVKKRISKVAKAT 184
              +      F  +MSN QTWVSAALT+E TCLDGF E  + G+VK  ++ ++  VA+ T
Sbjct: 126 RAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVT 185

Query: 185 SNALYMINR 187
           SNAL ++N+
Sbjct: 186 SNALALVNQ 194

[Close]

BLAST of Carg28176 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 8.0e-26
Identity = 76/187 (40.64%), Postives = 113/187 (60.43%), Query Frame = 0


Query: 6   LLLVLLLSLSLSAAAHDGPPPSQ---DLIHSSCLQASYPSLCIRTLSSYSGAV-NTPQDL 65
           +L VL LS +    A +    S    + I +SC   SYP  C ++LSSY+  +   P+ L
Sbjct: 10  ILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKL 69

Query: 66  AQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRH 125
           A+  L+VS++ A+    Y+S  + + +  +KRQ  AV DC+E++GD+V+ LSN+L  L+H
Sbjct: 70  AETALAVSIARAKSAKTYVSEMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKH 129

Query: 126 LPYGDR-RSFRLQMSNAQTWVSAALTNEDTCLDGF--KEVDGEVKFDVKKRISKVAKATS 185
           L  GD    F   +SN +TW SAALT+E  C+DGF  K + GE+K  ++  I  VA+ TS
Sbjct: 130 LEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETS 189

[Close]

BLAST of Carg28176 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.3e-25
Identity = 68/186 (36.56%), Postives = 114/186 (61.29%), Query Frame = 0


Query: 3   SLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAV-NTPQDL 62
           S S L+   L + L+AA+     P+   I +SC   +YP++C ++LS+Y+  + N PQ+L
Sbjct: 4   SKSTLVFSALLVILAAAS---AAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQEL 63

Query: 63  AQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRH 122
           A   L VSL+  +    ++   +   +    RQ AA+ DC+E++ DS++ +S +   +++
Sbjct: 64  ASTALQVSLTRTQQAQTFMKRLN-KFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKN 123

Query: 123 LPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGF--KEVDGEVKFDVKKRISKVAKATSN 182
           L +     F  +MSN +TWVSAALT+E TC+DGF  K +DG++K  V+ ++  VA+ TSN
Sbjct: 124 LSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSN 183

Query: 183 ALYMIN 186
           AL ++N
Sbjct: 184 ALALVN 185

[Close]

BLAST of Carg28176 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.0e-25
Identity = 74/194 (38.14%), Postives = 109/194 (56.19%), Query Frame = 0


Query: 14  LSLSAAAHDGPPPSQ---------------------DLIHSSCLQASYPSLCIRTLSSYS 73
           LSLS ++   PPPS                      D I +SC    Y ++C  +LS Y+
Sbjct: 110 LSLSPSSPPPPPPSSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYA 169

Query: 74  GAV-NTPQDLAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEE 133
             + + PQ LA   L+++LS A+  S+++ N S      ++ +  AV DCVE+IGDSV  
Sbjct: 170 STIRSNPQKLAVIALNLTLSSAKSASKFVKNISHG-GGLTRLEVVAVADCVEEIGDSVTS 229

Query: 134 LSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRIS 186
           L +++  L  + Y D   F + MS+ +TWVSAALTN+DTC+DGF  V   VK  V++ + 
Sbjct: 230 LQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGFSLVKTAVKDLVRRHVV 289

[Close]

BLAST of Carg28176 vs. ExPASy TrEMBL
Match: A0A6J1H9I1 (pectinesterase inhibitor 3 OS=Cucurbita moschata OX=3662 GN=LOC111461775 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.4e-102
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD
Sbjct: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180
           HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA
Sbjct: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180

Query: 181 LYMINRLDVGTPAGRKKLTAP 202
           LYMINRLDVGTPAGRKKLTAP
Sbjct: 181 LYMINRLDVGTPAGRKKLTAP 201

[Close]

BLAST of Carg28176 vs. ExPASy TrEMBL
Match: A0A6J1JAB3 (pectinesterase inhibitor 3 OS=Cucurbita maxima OX=3661 GN=LOC111484974 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.1e-99
Identity = 196/201 (97.51%), Postives = 197/201 (98.01%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MTSLSLLL LLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD
Sbjct: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQATLSVSLSLARDLSRYLSNSS NLRQASKRQR AVDDCVEQIGDSVEELSNTLGLLR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSVNLRQASKRQRVAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180
           HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA
Sbjct: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180

Query: 181 LYMINRLDVGTPAGRKKLTAP 202
           LYMINRL+VGTPAGRKKL AP
Sbjct: 181 LYMINRLNVGTPAGRKKLAAP 201

[Close]

BLAST of Carg28176 vs. ExPASy TrEMBL
Match: A0A0A0LWR8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G597770 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 5.9e-72
Identity = 157/199 (78.89%), Postives = 173/199 (86.93%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT LSLLL LL SLSLSAA       SQDLIHSSCLQASYP+LCIRTLSSY+GAV TP+D
Sbjct: 28  MTVLSLLL-LLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 87

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQAT+SVSLSLA++LS YLS+S   LRQAS++QRAAVDDCV+QIGDSVEELSNTLG+LR
Sbjct: 88  LAQATISVSLSLAQNLSEYLSDS---LRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLR 147

Query: 121 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           HLP G DRR FRL+M NA+TWVSAALTNE+TCLDGFKEVDGEVK DVK+RI KVAK TSN
Sbjct: 148 HLPCGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSN 207

Query: 181 ALYMINRLDVGTPAGRKKL 199
           AL+MINRLD G P GRK L
Sbjct: 208 ALFMINRLDGGNPTGRKML 222

[Close]

BLAST of Carg28176 vs. ExPASy TrEMBL
Match: A0A5A7UKZ9 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001300 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 3.2e-70
Identity = 150/197 (76.14%), Postives = 171/197 (86.80%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT LSLLL+LLLSLSLSAA   G   SQDLI SSCLQA YP+LCIRTLSSY+G+V TP+D
Sbjct: 1   MTVLSLLLLLLLSLSLSAAHGGGGAVSQDLIRSSCLQARYPTLCIRTLSSYAGSVKTPRD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQ T+SVSLSLA++LS YLS+S   LR+AS++QRAAVDDCV+QIGDSVEELSNTLG+LR
Sbjct: 61  LAQVTISVSLSLAQNLSEYLSDS---LRKASRQQRAAVDDCVDQIGDSVEELSNTLGVLR 120

Query: 121 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           HLP G DRR FRL+M NA+TWVSAALTNE+TCLDGFKEVDGEVK DVK+RI KVAK TSN
Sbjct: 121 HLPCGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRIVKVAKVTSN 180

Query: 181 ALYMINRLDVGTPAGRK 197
           AL+MINRLD G   G++
Sbjct: 181 ALFMINRLDSGNSTGKE 194

[Close]

BLAST of Carg28176 vs. ExPASy TrEMBL
Match: A0A1S3BR62 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492396 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 3.2e-70
Identity = 150/197 (76.14%), Postives = 171/197 (86.80%), Query Frame = 0


Query: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT LSLLL+LLLSLSLSAA   G   SQDLI SSCLQA YP+LCIRTLSSY+G+V TP+D
Sbjct: 1   MTVLSLLLLLLLSLSLSAAHGGGGAVSQDLIRSSCLQARYPTLCIRTLSSYAGSVKTPRD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQ T+SVSLSLA++LS YLS+S   LR+AS++QRAAVDDCV+QIGDSVEELSNTLG+LR
Sbjct: 61  LAQVTISVSLSLAQNLSEYLSDS---LRKASRQQRAAVDDCVDQIGDSVEELSNTLGVLR 120

Query: 121 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           HLP G DRR FRL+M NA+TWVSAALTNE+TCLDGFKEVDGEVK DVK+RI KVAK TSN
Sbjct: 121 HLPCGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRIVKVAKVTSN 180

Query: 181 ALYMINRLDVGTPAGRK 197
           AL+MINRLD G   G++
Sbjct: 181 ALFMINRLDSGNSTGKE 194

[Close]

BLAST of Carg28176 vs. TAIR 10
Match: AT5G20740.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 173.3 bits (438), Expect = 1.9e-43
Identity = 96/190 (50.53%), Postives = 138/190 (72.63%), Query Frame = 0


Query: 3   SLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSG-AVNTPQDL 62
           +L L+ +    +  ++  H     ++D++HSSC  ASYPSLC+RTLSSYSG  +   +DL
Sbjct: 6   NLFLVAIAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDL 65

Query: 63  AQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRH 122
           AQA + +SLS A+  ++ L+    ++ +  K+++AA+ DCVE IGDSV+ELS TLG+L+H
Sbjct: 66  AQAAIKISLSHAQSAAKKLAVVRDSVGK-KKQEKAALVDCVEMIGDSVDELSRTLGVLKH 125

Query: 123 LPY--GDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEV-DGEVKFDVKKRISKVAKATS 182
           L    G  + FR QMSNAQTW SAALT++DTCLDGF+ + DGE+K +VK+ ++KVA+ TS
Sbjct: 126 LRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTS 185

Query: 183 NALYMINRLD 189
           NALYM+N+LD
Sbjct: 186 NALYMVNQLD 194

[Close]

BLAST of Carg28176 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 8.7e-28
Identity = 73/189 (38.62%), Postives = 118/189 (62.43%), Query Frame = 0


Query: 5   SLLLVLLLSLSL--SAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAV--NTPQD 64
           ++ LV+LLS+++  S++A      S   I SSC    YPSLC+ TLS+Y+  +  N  QD
Sbjct: 6   TIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQD 65

Query: 65  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 124
           LAQ  L +SL+ A+ ++ +++  +    +  +R+  A+ DC+E +G+SV+ L+ ++  L 
Sbjct: 66  LAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELA 125

Query: 125 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKE--VDGEVKFDVKKRISKVAKAT 184
              +      F  +MSN QTWVSAALT+E TCLDGF E  + G+VK  ++ ++  VA+ T
Sbjct: 126 RAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVT 185

Query: 185 SNALYMINR 187
           SNAL ++N+
Sbjct: 186 SNALALVNQ 194

[Close]

BLAST of Carg28176 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 5.7e-27
Identity = 76/187 (40.64%), Postives = 113/187 (60.43%), Query Frame = 0


Query: 6   LLLVLLLSLSLSAAAHDGPPPSQ---DLIHSSCLQASYPSLCIRTLSSYSGAV-NTPQDL 65
           +L VL LS +    A +    S    + I +SC   SYP  C ++LSSY+  +   P+ L
Sbjct: 10  ILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKL 69

Query: 66  AQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRH 125
           A+  L+VS++ A+    Y+S  + + +  +KRQ  AV DC+E++GD+V+ LSN+L  L+H
Sbjct: 70  AETALAVSIARAKSAKTYVSEMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKH 129

Query: 126 LPYGDR-RSFRLQMSNAQTWVSAALTNEDTCLDGF--KEVDGEVKFDVKKRISKVAKATS 185
           L  GD    F   +SN +TW SAALT+E  C+DGF  K + GE+K  ++  I  VA+ TS
Sbjct: 130 LEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETS 189

[Close]

BLAST of Carg28176 vs. TAIR 10
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 117.9 bits (294), Expect = 9.7e-27
Identity = 81/190 (42.63%), Postives = 112/190 (58.95%), Query Frame = 0


Query: 6   LLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAV-NTPQDLAQA 65
           L  VL L L++ + + D      D I +SC    YP LC  +LSS+S +V N P  LA+A
Sbjct: 14  LASVLPLILTVHSQSDD-----SDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARA 73

Query: 66  TLSVSLSLARDLSRYLSN-----SSANLRQASKRQRAAV-DDCVEQIGDSVEELSNTLGL 125
            +SV+L+   DL+ YL+N       +N   A     AAV  DC + + D+VEE+  ++  
Sbjct: 74  AISVTLTKTLDLASYLANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQ 133

Query: 126 LRHL-PYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEV-KFDVKKRISKVAKA 185
           +R L   G   SFR QMSN QTW+SAALT+E+TC DGFK++  E  K D+  R+  V K 
Sbjct: 134 MRELVSTGSLESFRFQMSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKM 193

Query: 186 TSNALYMINR 187
           TSNAL ++NR
Sbjct: 194 TSNALALVNR 198

[Close]

BLAST of Carg28176 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 2.2e-26
Identity = 74/194 (38.14%), Postives = 109/194 (56.19%), Query Frame = 0


Query: 14  LSLSAAAHDGPPPSQ---------------------DLIHSSCLQASYPSLCIRTLSSYS 73
           LSLS ++   PPPS                      D I +SC    Y ++C  +LS Y+
Sbjct: 110 LSLSPSSPPPPPPSSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYA 169

Query: 74  GAV-NTPQDLAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEE 133
             + + PQ LA   L+++LS A+  S+++ N S      ++ +  AV DCVE+IGDSV  
Sbjct: 170 STIRSNPQKLAVIALNLTLSSAKSASKFVKNISHG-GGLTRLEVVAVADCVEEIGDSVTS 229

Query: 134 LSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRIS 186
           L +++  L  + Y D   F + MS+ +TWVSAALTN+DTC+DGF  V   VK  V++ + 
Sbjct: 230 LQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGFSLVKTAVKDLVRRHVV 289

The following BLAST results are available for this feature:

BLAST of Carg28176 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961187.1	5.0e-102	100.00	pectinesterase inhibitor 3 [Cucurbita moschata] >KAG6590055.1 Pectinesterase inh...	[more]
XP_023516503.1	7.3e-101	99.00	pectinesterase inhibitor 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022987422.1	2.3e-99	97.51	pectinesterase inhibitor 3 [Cucurbita maxima]	[more]
XP_038879670.1	7.3e-77	81.68	pectinesterase inhibitor 3 [Benincasa hispida]	[more]
XP_004144132.2	1.2e-71	78.89	pectinesterase inhibitor 3 [Cucumis sativus] >KGN66330.1 hypothetical protein Cs...	[more]

BLAST of Carg28176 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WE4	2.7e-42	50.53	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]
Q9SI72	1.2e-26	38.62	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SB37	8.0e-26	40.64	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
P17407	2.3e-25	36.56	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI74	3.0e-25	38.14	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

BLAST of Carg28176 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H9I1	2.4e-102	100.00	pectinesterase inhibitor 3 OS=Cucurbita moschata OX=3662 GN=LOC111461775 PE=4 SV...	[more]
A0A6J1JAB3	1.1e-99	97.51	pectinesterase inhibitor 3 OS=Cucurbita maxima OX=3661 GN=LOC111484974 PE=4 SV=1	[more]
A0A0A0LWR8	5.9e-72	78.89	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G597770 PE=4 S...	[more]
A0A5A7UKZ9	3.2e-70	76.14	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001300...	[more]
A0A1S3BR62	3.2e-70	76.14	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492396 PE=4 SV=1	[more]

BLAST of Carg28176 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20740.1	1.9e-43	50.53	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	8.7e-28	38.62	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	5.7e-27	40.64	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G23205.1	9.7e-27	42.63	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	2.2e-26	38.14	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 26..183 e-value: 2.6E-43 score: 159.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 29..182 e-value: 3.9E-35 score: 121.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..187 e-value: 7.3E-36 score: 121.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 26..188 e-value: 1.9E-39 score: 137.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..187
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 4..189
None	No IPR available	PANTHER	PTHR31080:SF110	PECTINESTERASE INHIBITOR 3	coord: 4..189
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 33..188 e-value: 5.86664E-43 score: 139.496

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28176-RA	Carg28176-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0004857	enzyme inhibitor activity