Carg27916 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27916
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 6.3-like
LocationCarg_Chr15: 8149083 .. 8152223 (+)
RNA-Seq ExpressionCarg27916
SyntenyCarg27916
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTATTCTCCCTGAAACAACTCATGAAAAAACCCTCTCAGATGCCTGGGACTACACCGGCAAGCCTGCCATTAAGGCCAAAACCGGTGGCTGGGTCGCCGCCGCCATGATTCTAGGTCCATTATCATTAATCTTCCCTTTCTATAAGGCAAAGCTTAAGCCTTTTAACTCATTAGTTCTTCAAAAAATCTGCAGGGCCTGAAGGGGTTGAGAGGCTTACAACGCTGGGAATTGCAGTCAATTTGGTCACTTATTTGACTGAAACAATGCATCTCGGTAATGCTACCGCCGCCAACAATGTCACCAACTTCCTCGGAACCTCCTTCATGCTATGTCTCTTCGGTGGCTTTGTCGCCGACACTTTTCTCGGCCGGTAATAACATCAATTTTTCAAAATTTTATTTATTTACTTTCATAAATTTCAATTTTTTCTTCCGTTTTTGTCGTGTTTAATTGATTTGGAATAATAGAAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTCTTAAAGGTATCTGACAATTTCCATCTTCGTAACGGTTCAAGCAATTGTAAGATTTATGTCGCTTTCTCGATATTAATCTTATTTTTATGTAAACATGTTTTAATTTAAATTTGTGGATTCAATTAGGGTGTAACTCTCTTAACCATCTCCACCATAATTCCAAGTCTCCGGCCGCCAAAATGTTCACCGGGGCTCTCACCAACTCCATGCATTCCGGCGGACAGCAAGCAGCTGGCAATTCTCCACACAGCACTATACTTAACGGCACTCGGCACCGGTGGCTTAAAATCGAGTGTTTCGGGGTTCGGTTCCGATCAATTCGACGAAACGGATAAGGAGGAGAGAGCAAAGACGGCGGTTTTCTTTAATTGGTTCTTCTTCTTGATAAGCATTGGATCACTTGGAGCTGTGACAATTCTTGTTTATATTCAAGATAATTGGGGCAGGCAATGGGGATATGGGATATGTGCTTGTGCCATTGTGTTGGGATTGGTTGTGTTCCTGTCCGGGACTCGACGATACCGGTTTAAGAAATTGGTCGGTAGCCCGTTGACACAAATCGCCGCCGTGTTCGTTGCAGCATGGAGGAAGAGGAGGTTGGATTTGCCCTCGGATCCATCCGCGTTGTACCAAATCGAAGACGCCGTGGATGGAGCTGGGAAGAAGAAGCAGAAGTTGCCCCGCAGCAAGCAGTTCAGGTTGGTTTATTAGTTTATTCAGTTTTAACGCTTTAAAAAAGTCAACTACCATAATTTTTTTAACTCCTAATAATACCTTAATTTTGTATATTTTAAAAATAACATTAAAACTTTCCAAAGAAAGAAATTTAAAAAGTTATTGCCAATAATATTTGGACAAAAATCGTCTATTTATACTTCATAGATCACTTTCTTCCTCAGACTTTTAAAACAAGTTTAAGTTAAGGATATTAACGTTACTTTTGAAAGTGTATAGGTGTCTTTTTTTGTACGAACGGTAATGATATTTTTTTAACTTTAAAAAACTTATGGATATTAATGAAACTTTTGAATTTCGAGTATTTTCTAGTTCAATAGTATTTTTGAAACAAATTATTAATGATTTTCATCTAGAACTAAGGATGAATGTATTTTTGATACGTGGTGATACTACGACTAAAATAGTTGATATAATGGGTATAATATATGATAATTTATTTAAATAAACTTACTTGTAAAGATGATTAATGAATTATTACATAGTTTTCGTGGGCATTCGGAATACGTTATTGTACCATATAAGCCAAGAAAATTGAATCCCAAATTCTACTTTTGGTCACCAATTATAGACTTTAATAACTTAAAAACTATATCCCATCCAACAGGAACCCGACAAGCTTAGGACAAAAAACATTTTCAACACTTGGAAGTTTGCCATAATTGAGCTTATTATATTTTAGTATTTTCAATTTTCACACAAAGAAATAAATTTATTATAAAGTTAGGCATCTAGCCGACAATTTTTATCATTATTCTATTATATATAACTCAATTGATTGGCTCTCCAAGTTGTGTGAAAAAGAATTAAAACAAATCAAAATGACCAAAAAGTTGAAAACTTTGGTTGAACCCAAGTTTGAATTCGACCAAAAATCAGTAGATAATCATGAAATTATGTTAAAGATTACATTTTTATATTGAATTTGTTGGTTTTTTGTGTAGGTTTTTGGATAAGGCAGCAATCAAGGATGAAGAAATAGTTGGGAATGTTGTGAACAAGTGGAATTTATCAACCCTAACAGACATTGAGGAAGTTAAATTAGTGCTTAGAATGTTACCAATTTGGGCAACCACCATTATTTTCTGGACAGTCTATGCTCAAATGACAACATTCTCTGTCTCCCAAGCCACTACTATGAACCGACACATTGGAAAATCATTTCAAATTCCGGCAGCCTCCCTCACTGCCTTCTTCGTTGCTAGCATCCTCGTAACCGTCCCGATCTACGACCAACTCATCGTCCCAATAGCATCAAGAATTTTGAAGAACCCACAAGGGCTAACCCCACTGCAACGAGTTGGGGTTGGGTTGGTTCTATCGATAATTGCCATGGTCGCCGCCGCGCTTACCGAAATCAAACGATTGAGGGTGGTGGAGGAAAATGGATTGGTGTTCAAGCCAATGGCTGAGGTGCCAATGAGTGTGTTTTGGCTTTGTCCACAGTTCTTAATTGTGGGAGCAGGTGAGAAAATTTCCCTCATTTTGGCATTTTGGTTCGATTATATGTAAAAAGTTAGAACGTAAAAATTTTGATCGTTTGTGTATGCAGGCGAGGCGTTGACGTACATGGGGCAATTGGATTTTTTCTTAAGAGAGTGCCCTAAAGGGATGAAGACAATGAGCACAGGGCTGTTCTTGAGCACACTTGCTTTAGGGTTCTTCTTTAGCTCATTATTGGTTACAATAGTGGGAAAAGTGACTGAAAATGGGCATCCATGGATTCCGGACAATCTCAATGAAGGGAAGCTTTATGATTTCTATTGGCTGTTGGCGATCTTAAGTGTGTTGAATTTGGCGGTGTTCTTGATTTGTGCTAAGTGGTATGTGTACAAGGAGAAGAGGTTGGCTGATGAGGGCATTGAATTGGAGGACTGTGGCCCATCTGCCCATTAA

mRNA sequence

ATGAGTATTCTCCCTGAAACAACTCATGAAAAAACCCTCTCAGATGCCTGGGACTACACCGGCAAGCCTGCCATTAAGGCCAAAACCGGTGGCTGGGTCGCCGCCGCCATGATTCTAGGGCCTGAAGGGGTTGAGAGGCTTACAACGCTGGGAATTGCAGTCAATTTGGTCACTTATTTGACTGAAACAATGCATCTCGGTAATGCTACCGCCGCCAACAATGTCACCAACTTCCTCGGAACCTCCTTCATGCTATGTCTCTTCGGTGGCTTTGTCGCCGACACTTTTCTCGGCCGGTATCTGACAATTTCCATCTTCGTAACGGTTCAAGCAATTGGTGTAACTCTCTTAACCATCTCCACCATAATTCCAAGTCTCCGGCCGCCAAAATGTTCACCGGGGCTCTCACCAACTCCATGCATTCCGGCGGACAGCAAGCAGCTGGCAATTCTCCACACAGCACTATACTTAACGGCACTCGGCACCGGTGGCTTAAAATCGAGTGTTTCGGGGTTCGGTTCCGATCAATTCGACGAAACGGATAAGGAGGAGAGAGCAAAGACGGCGGTTTTCTTTAATTGGTTCTTCTTCTTGATAAGCATTGGATCACTTGGAGCTGTGACAATTCTTGTTTATATTCAAGATAATTGGGGCAGGCAATGGGGATATGGGATATGTGCTTGTGCCATTGTGTTGGGATTGGTTGTGTTCCTGTCCGGGACTCGACGATACCGGTTTAAGAAATTGGTCGGTAGCCCGTTGACACAAATCGCCGCCGTGTTCGTTGCAGCATGGAGGAAGAGGAGGTTGGATTTGCCCTCGGATCCATCCGCGTTGTACCAAATCGAAGACGCCGTGGATGGAGCTGGGAAGAAGAAGCAGAAGTTGCCCCGCAGCAAGCAGTTCAGGTTTTTGGATAAGGCAGCAATCAAGGATGAAGAAATAGTTGGGAATGTTGTGAACAAGTGGAATTTATCAACCCTAACAGACATTGAGGAAGTTAAATTAGTGCTTAGAATGTTACCAATTTGGGCAACCACCATTATTTTCTGGACAGTCTATGCTCAAATGACAACATTCTCTGTCTCCCAAGCCACTACTATGAACCGACACATTGGAAAATCATTTCAAATTCCGGCAGCCTCCCTCACTGCCTTCTTCGTTGCTAGCATCCTCGTAACCGTCCCGATCTACGACCAACTCATCGTCCCAATAGCATCAAGAATTTTGAAGAACCCACAAGGGCTAACCCCACTGCAACGAGTTGGGGTTGGGTTGGTTCTATCGATAATTGCCATGGTCGCCGCCGCGCTTACCGAAATCAAACGATTGAGGGTGGTGGAGGAAAATGGATTGGTGTTCAAGCCAATGGCTGAGGTGCCAATGAGTGTGTTTTGGCTTTGTCCACAGTTCTTAATTGTGGGAGCAGGCGAGGCGTTGACGTACATGGGGCAATTGGATTTTTTCTTAAGAGAGTGCCCTAAAGGGATGAAGACAATGAGCACAGGGCTGTTCTTGAGCACACTTGCTTTAGGGTTCTTCTTTAGCTCATTATTGGTTACAATAGTGGGAAAAGTGACTGAAAATGGGCATCCATGGATTCCGGACAATCTCAATGAAGGGAAGCTTTATGATTTCTATTGGCTGTTGGCGATCTTAAGTGTGTTGAATTTGGCGGTGTTCTTGATTTGTGCTAAGTGGTATGTGTACAAGGAGAAGAGGTTGGCTGATGAGGGCATTGAATTGGAGGACTGTGGCCCATCTGCCCATTAA

Coding sequence (CDS)

ATGAGTATTCTCCCTGAAACAACTCATGAAAAAACCCTCTCAGATGCCTGGGACTACACCGGCAAGCCTGCCATTAAGGCCAAAACCGGTGGCTGGGTCGCCGCCGCCATGATTCTAGGGCCTGAAGGGGTTGAGAGGCTTACAACGCTGGGAATTGCAGTCAATTTGGTCACTTATTTGACTGAAACAATGCATCTCGGTAATGCTACCGCCGCCAACAATGTCACCAACTTCCTCGGAACCTCCTTCATGCTATGTCTCTTCGGTGGCTTTGTCGCCGACACTTTTCTCGGCCGGTATCTGACAATTTCCATCTTCGTAACGGTTCAAGCAATTGGTGTAACTCTCTTAACCATCTCCACCATAATTCCAAGTCTCCGGCCGCCAAAATGTTCACCGGGGCTCTCACCAACTCCATGCATTCCGGCGGACAGCAAGCAGCTGGCAATTCTCCACACAGCACTATACTTAACGGCACTCGGCACCGGTGGCTTAAAATCGAGTGTTTCGGGGTTCGGTTCCGATCAATTCGACGAAACGGATAAGGAGGAGAGAGCAAAGACGGCGGTTTTCTTTAATTGGTTCTTCTTCTTGATAAGCATTGGATCACTTGGAGCTGTGACAATTCTTGTTTATATTCAAGATAATTGGGGCAGGCAATGGGGATATGGGATATGTGCTTGTGCCATTGTGTTGGGATTGGTTGTGTTCCTGTCCGGGACTCGACGATACCGGTTTAAGAAATTGGTCGGTAGCCCGTTGACACAAATCGCCGCCGTGTTCGTTGCAGCATGGAGGAAGAGGAGGTTGGATTTGCCCTCGGATCCATCCGCGTTGTACCAAATCGAAGACGCCGTGGATGGAGCTGGGAAGAAGAAGCAGAAGTTGCCCCGCAGCAAGCAGTTCAGGTTTTTGGATAAGGCAGCAATCAAGGATGAAGAAATAGTTGGGAATGTTGTGAACAAGTGGAATTTATCAACCCTAACAGACATTGAGGAAGTTAAATTAGTGCTTAGAATGTTACCAATTTGGGCAACCACCATTATTTTCTGGACAGTCTATGCTCAAATGACAACATTCTCTGTCTCCCAAGCCACTACTATGAACCGACACATTGGAAAATCATTTCAAATTCCGGCAGCCTCCCTCACTGCCTTCTTCGTTGCTAGCATCCTCGTAACCGTCCCGATCTACGACCAACTCATCGTCCCAATAGCATCAAGAATTTTGAAGAACCCACAAGGGCTAACCCCACTGCAACGAGTTGGGGTTGGGTTGGTTCTATCGATAATTGCCATGGTCGCCGCCGCGCTTACCGAAATCAAACGATTGAGGGTGGTGGAGGAAAATGGATTGGTGTTCAAGCCAATGGCTGAGGTGCCAATGAGTGTGTTTTGGCTTTGTCCACAGTTCTTAATTGTGGGAGCAGGCGAGGCGTTGACGTACATGGGGCAATTGGATTTTTTCTTAAGAGAGTGCCCTAAAGGGATGAAGACAATGAGCACAGGGCTGTTCTTGAGCACACTTGCTTTAGGGTTCTTCTTTAGCTCATTATTGGTTACAATAGTGGGAAAAGTGACTGAAAATGGGCATCCATGGATTCCGGACAATCTCAATGAAGGGAAGCTTTATGATTTCTATTGGCTGTTGGCGATCTTAAGTGTGTTGAATTTGGCGGTGTTCTTGATTTGTGCTAAGTGGTATGTGTACAAGGAGAAGAGGTTGGCTGATGAGGGCATTGAATTGGAGGACTGTGGCCCATCTGCCCATTAA

Protein sequence

MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQRVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALTYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNEGKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH
Homology
BLAST of Carg27916 vs. NCBI nr
Match: KAG7016898.1 (Protein NRT1/ PTR FAMILY 6.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60
           MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL
Sbjct: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60

Query: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120
           TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS
Sbjct: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120

Query: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180
           TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET
Sbjct: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180

Query: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240
           DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG
Sbjct: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240

Query: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300
           TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK
Sbjct: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300

Query: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360
           QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF
Sbjct: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360

Query: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQ 420
           SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQ
Sbjct: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQ 420

Query: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480
           RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL
Sbjct: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480

Query: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540
           TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE
Sbjct: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540

Query: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 591
           GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH
Sbjct: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 590

BLAST of Carg27916 vs. NCBI nr
Match: KAG6579420.1 (Protein NRT1/ PTR FAMILY 6.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 588/590 (99.66%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60
           MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL
Sbjct: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60

Query: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120
           TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS
Sbjct: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120

Query: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180
           TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET
Sbjct: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180

Query: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240
           DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG
Sbjct: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240

Query: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300
           TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK
Sbjct: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300

Query: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360
           QFRFLDKAAIKDEEIVGNVVNKWN+STLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF
Sbjct: 301 QFRFLDKAAIKDEEIVGNVVNKWNISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360

Query: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQ 420
           SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYD+LIVPIASRILKNPQGLTPLQ
Sbjct: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDRLIVPIASRILKNPQGLTPLQ 420

Query: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480
           RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL
Sbjct: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480

Query: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540
           TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE
Sbjct: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540

Query: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 591
           GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH
Sbjct: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 590

BLAST of Carg27916 vs. NCBI nr
Match: XP_022922301.1 (protein NRT1/ PTR FAMILY 6.3-like [Cucurbita moschata])

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 588/590 (99.66%), Postives = 589/590 (99.83%), Query Frame = 0

Query: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60
           MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL
Sbjct: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60

Query: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120
           TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS
Sbjct: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120

Query: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180
           TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDE 
Sbjct: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDEA 180

Query: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240
           DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG
Sbjct: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240

Query: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300
           TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK
Sbjct: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300

Query: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360
           QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF
Sbjct: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360

Query: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQ 420
           SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYD+LIVPIASRILKNPQGLTPLQ
Sbjct: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDRLIVPIASRILKNPQGLTPLQ 420

Query: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480
           RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL
Sbjct: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480

Query: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540
           TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE
Sbjct: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540

Query: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 591
           GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH
Sbjct: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 590

BLAST of Carg27916 vs. NCBI nr
Match: XP_022969890.1 (protein NRT1/ PTR FAMILY 6.3-like [Cucurbita maxima])

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 580/590 (98.31%), Postives = 588/590 (99.66%), Query Frame = 0

Query: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60
           MSILP+TTHEKT+SDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL
Sbjct: 1   MSILPQTTHEKTVSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60

Query: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120
           TETMHLGNATAANNVTNFLGTSFMLCL GGFVADTFLGRYLTISIFV VQAIGVTLLTIS
Sbjct: 61  TETMHLGNATAANNVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFVIVQAIGVTLLTIS 120

Query: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180
           TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFD+T
Sbjct: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDDT 180

Query: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240
           DKEERAKTAVFFNWFFFLISIGSLGAVT+LVYIQDNWGRQWGYGICACAIVLGLVVFLSG
Sbjct: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTLLVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240

Query: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300
           TRRYRFKK+VGSPLTQIAAVFVAAWRKRR+DLPSDPSALYQIEDAVDGAGKKKQKLPRSK
Sbjct: 241 TRRYRFKKMVGSPLTQIAAVFVAAWRKRRMDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300

Query: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360
           QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF
Sbjct: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360

Query: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQ 420
           SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYD+LIVPIASRILKNPQGLTPLQ
Sbjct: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDRLIVPIASRILKNPQGLTPLQ 420

Query: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480
           RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLV+KPMAEVPMSVFWLCPQFLIVGAGEAL
Sbjct: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVYKPMAEVPMSVFWLCPQFLIVGAGEAL 480

Query: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540
           TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE
Sbjct: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540

Query: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 591
           GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH
Sbjct: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 590

BLAST of Carg27916 vs. NCBI nr
Match: XP_023550325.1 (protein NRT1/ PTR FAMILY 6.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 581/587 (98.98%), Postives = 583/587 (99.32%), Query Frame = 0

Query: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60
           MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL
Sbjct: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60

Query: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120
           TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIF TVQAIGVTLLTIS
Sbjct: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAIGVTLLTIS 120

Query: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180
           TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET
Sbjct: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180

Query: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240
           DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG
Sbjct: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240

Query: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300
           TRRYRFKKLVGSPLTQIAAVFVAAWRKRR+DLPSDPSALYQIEDAVDGAGKKKQKLPRSK
Sbjct: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRMDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300

Query: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360
           QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF
Sbjct: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360

Query: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQ 420
           SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYD+LIVPIASRILKNPQGLTPLQ
Sbjct: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDRLIVPIASRILKNPQGLTPLQ 420

Query: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480
           RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL
Sbjct: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480

Query: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540
           TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE
Sbjct: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540

Query: 541 GKLYDFYWLLAILSVLNLAVFLICAKWY---VYKEKRLADEGIELED 585
           GKLYDFYWLLAILSVLNLAVFLICAKWY   VYKEKRLADEGIELED
Sbjct: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYXNXVYKEKRLADEGIELED 587

BLAST of Carg27916 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 3.0e-214
Identity = 378/585 (64.62%), Postives = 465/585 (79.49%), Query Frame = 0

Query: 4   LPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTET 63
           LPET  +  L DAWD+ G+PA ++KTGGW +AAMIL  E VERLTTLGI VNLVTYLT T
Sbjct: 3   LPETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGT 62

Query: 64  MHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTII 123
           MHLGNATAAN VTNFLGTSFMLCL GGF+ADTFLGRYLTI+IF  +QA GV++LT+STII
Sbjct: 63  MHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTII 122

Query: 124 PSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKE 183
           P LRPP+C+P  S + C  A   QL +L+ ALYLTALGTGG+K+SVSGFGSDQFDET+ +
Sbjct: 123 PGLRPPRCNPTTS-SHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPK 182

Query: 184 ERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRR 243
           ER+K   FFN FFF I++GSL AVT+LVY+QD+ GR+WGYGICA AIVL L VFL+GT R
Sbjct: 183 ERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNR 242

Query: 244 YRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIED--AVDGAGKKKQKLPRSKQ 303
           YRFKKL+GSP+TQ+AAV VAAWR R+L+LP+DPS LY ++D  A +G+ K KQKLP ++Q
Sbjct: 243 YRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQ 302

Query: 304 FRFLDKAAIKDEE--IVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTT 363
           FR LDKAAI+D+E  +  NV NKW LSTLTD+EEVK ++RMLPIWAT I+FWTV+AQ+TT
Sbjct: 303 FRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTT 362

Query: 364 FSVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPL 423
            SV+Q+ T++R IG SF+IP AS+  F+V  +L+T  +YD++ + +  ++   P GL PL
Sbjct: 363 LSVAQSETLDRSIG-SFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPL 422

Query: 424 QRVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEA 483
           QR+G+GL    +AM  AAL E+KRLR    +G   K +   P+  + L PQ+LIVG GEA
Sbjct: 423 QRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTL---PLGFYLLIPQYLIVGIGEA 482

Query: 484 LTYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLN 543
           L Y GQLDFFLRECPKGMK MSTGL LSTLALGFFFSS+LVTIV K T   HPWI D+LN
Sbjct: 483 LIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLN 542

Query: 544 EGKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELED 585
           +G+LY+FYWL+A+L  LN  +FL+ +KWYVYKEKRLA+ GIEL+D
Sbjct: 543 KGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDD 582

BLAST of Carg27916 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 6.5e-169
Identity = 325/590 (55.08%), Postives = 420/590 (71.19%), Query Frame = 0

Query: 12  TLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTETMHLGNATA 71
           T++DA DY G+PA K+KTGGW+ AA+ILG E VERL+T+GIAVNLVTYL ETMHL ++T+
Sbjct: 8   TVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTS 67

Query: 72  ANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTIIPSLRPPKC 131
           AN VT+F+GTSF+LCL GGF+AD+FLGR+ TI IF T+QA+G   L ++T +P LRPP C
Sbjct: 68  ANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTC 127

Query: 132 SPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKEERAKTAVF 191
             G     CIPA + Q+ IL+ +LYL ALGTGGLKSS+SGFGSDQFD+ D +E+A  A F
Sbjct: 128 HHG---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFF 187

Query: 192 FNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRRYRFKKLVG 251
           FN FFF IS+G+L AVT+LVY+QD  GR W YGIC  ++ + +V+FL GT+RYR+KK  G
Sbjct: 188 FNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQG 247

Query: 252 SPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQFRFLDKAAIK 311
           SP+ QI  V  AA+RKR+++LP   S +Y  ED  +G      ++  + QF  LDKAAI 
Sbjct: 248 SPVVQIFQVIAAAFRKRKMELPQ--SIVYLYEDNPEGI-----RIEHTDQFHLLDKAAIV 307

Query: 312 -----DEEIVGNVV-NKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQA 371
                ++ + G  + N W LS++T +EEVK+++R+LPIWATTIIFWT YAQM TFSV QA
Sbjct: 308 AEGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQA 367

Query: 372 TTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQRVGVG 431
           +TM R+IG SF+IPA SLT FFVA+IL+T+ +YD+ I+P   +  K   G + LQR+ +G
Sbjct: 368 STMRRNIG-SFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIG 427

Query: 432 LVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALTYMGQ 491
           LVLS   M AAAL E KRL V + +         +P+SVF L PQF +VGAGEA  Y GQ
Sbjct: 428 LVLSTAGMAAAALVEQKRLSVAKSSS-----QKTLPISVFLLVPQFFLVGAGEAFIYTGQ 487

Query: 492 LDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHP--WIPDNLNEGKL 551
           LDFF+ + PKGMKTMSTGLFL+TL+LGFF SS LV+IV +VT       W+ DN+N G+L
Sbjct: 488 LDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRL 547

Query: 552 YDFYWLLAILSVLNLAVFLICAKWY--------VYKEKRLADEGIELEDC 586
             FYWLL ILS +N  V++ICA W+        V KE     +G  +EDC
Sbjct: 548 DYFYWLLVILSGINFVVYIICALWFKPTKGKDSVEKEN---GKGFSVEDC 577

BLAST of Carg27916 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 2.0e-141
Identity = 275/563 (48.85%), Postives = 374/563 (66.43%), Query Frame = 0

Query: 15  DAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTETMHLGNATAANN 74
           +A+DY G P  K+KTGGW+ A +ILG E  ER+  +GI++NLVTYL   +H+ +A +A  
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 75  VTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTIIPSLRPPKCSP- 134
           VTNF+GT  +L L GGF+AD  LGRY  ++I  +V A+GV LLT++T I S+RPP C   
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 135 GLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKEERAKTAVFFN 194
                 CI A+  QLA+L+ ALY  ALG GG+KS+VSGFGSDQFD +D +E  +   FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 195 WFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRRYRFKKLVGSP 254
            F+F IS+GSL AV  LVY+QDN GR WGYGI A  +V+  +V L GT+RYRFKK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 255 LTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQFRFLDKAAIKDE 314
            T I  V   AW+KR+   P+ PS L          G     +P ++  + LDKAAI   
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL---------NGYDNTTVPHTEMLKCLDKAAISKN 315

Query: 315 EIVGN-----VVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTM 374
           E   +       + W +ST+T +EEVKLV++++PIWAT I+FWT+Y+QMTTF+V QAT M
Sbjct: 316 ESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFM 375

Query: 375 NRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQRVGVGLVL 434
           +R +G SF +PA S +AF + +IL+   + +++ VP+  R+ K PQG+T LQR+GVGLV 
Sbjct: 376 DRKLG-SFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVF 435

Query: 435 SIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALTYMGQLDF 494
           S+ AM  AA+ E  R      N        +  +S FWL PQ+ +VGAGEA  Y+GQL+F
Sbjct: 436 SMAAMAVAAVIENARREAAVNN--------DKKISAFWLVPQYFLVGAGEAFAYVGQLEF 495

Query: 495 FLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNEGKLYDFYW 554
           F+RE P+ MK+MSTGLFLST+++GFF SSLLV++V +VT+    W+  NLN+ +L  FYW
Sbjct: 496 FIREAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKS--WLRSNLNKARLNYFYW 555

Query: 555 LLAILSVLNLAVFLICAKWYVYK 572
           LL +L  LN  +F++ A  + YK
Sbjct: 556 LLVVLGALNFLIFIVFAMKHQYK 558

BLAST of Carg27916 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 7.6e-133
Identity = 257/559 (45.97%), Postives = 366/559 (65.47%), Query Frame = 0

Query: 18  DYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTETMHLGNATAANNVTN 77
           D    PA K KTG W A   ILG E  ERL   G+  NLV YL   ++ GNATAANNVTN
Sbjct: 14  DIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTN 73

Query: 78  FLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTIIPSLRPPKCSPGLSP 137
           + GT ++  L G F+AD +LGRY TI+ FV +   G+TLLT+S  +P L+P  C    + 
Sbjct: 74  WSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNC----NA 133

Query: 138 TPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKEERAKTAVFFNWFFF 197
             C P +S Q A+   ALY+ ALGTGG+K  VS FG+DQFDE D+ E+ K + FFNWF+F
Sbjct: 134 DTCHP-NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYF 193

Query: 198 LISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRRYRFKKLVGSPLTQI 257
            I++G+L A T+LV+IQ N G  WG+G+   A+V+ +  F  G+R YR ++  GSPLT+I
Sbjct: 194 SINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRI 253

Query: 258 AAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQFRFLDKAAI--KDEEI 317
             V VAA+RK  + +P D S L++  D      K  +KL  +   +F DKAA+  + + I
Sbjct: 254 FQVIVAAFRKISVKVPEDKSLLFETADDESNI-KGSRKLVHTDNLKFFDKAAVESQSDSI 313

Query: 318 VGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTMNRHIGKS 377
               VN W L ++T +EE+K ++ +LP+WAT I+F TVY+QM+T  V Q  TM++H+GK+
Sbjct: 314 KDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKN 373

Query: 378 FQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQRVGVGLVLSIIAMVA 437
           F+IP+ASL+ F   S+L   P+YDQ I+P+A +  +N +G T LQR+G+GLV+SI AM+ 
Sbjct: 374 FEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMIT 433

Query: 438 AALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALTYMGQLDFFLRECPK 497
           A + E+ RL  V+ +        ++ MS+FW  PQ+L++G  E  T++GQL+FF  + P 
Sbjct: 434 AGVLEVVRLDYVKTHNAY--DQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPD 493

Query: 498 GMKTMSTGLFLSTLALGFFFSSLLVTIVGKVT-ENGHP-WIPDNLNEGKLYDFYWLLAIL 557
            M+++ + L L+T+ALG + S++LVT+V K+T +NG P WIPDNLN G L  F++LLA L
Sbjct: 494 AMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATL 553

Query: 558 SVLNLAVFLICAKWYVYKE 573
           S LN  V+L  +K Y YK+
Sbjct: 554 SFLNFLVYLWISKRYKYKK 564

BLAST of Carg27916 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 9.9e-125
Identity = 247/559 (44.19%), Postives = 360/559 (64.40%), Query Frame = 0

Query: 18  DYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTETMHLGNATAANNVTN 77
           D   KPA K KTG W A   ILG E  ERL   G++ NL+ YL + M++ N +A+ +V+N
Sbjct: 15  DIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSN 74

Query: 78  FLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTIIPSLRPPKCSPGLSP 137
           + GT +   L G F+AD +LGRY TI+ FV +   G+TLLTIS  +P L     +P  S 
Sbjct: 75  WSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-----TPTCSG 134

Query: 138 TPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKEERAKTAVFFNWFFF 197
             C  A + Q AI   ALYL ALGTGG+K  VS FG+DQFD+TD++E+   + FFNWF+F
Sbjct: 135 ETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYF 194

Query: 198 LISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRRYRFKKLVGSPLTQI 257
           +I++G++ A ++LV+IQ N G  WG G+   A+ + +V F +G+  YR +K  GSPLT++
Sbjct: 195 VINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRM 254

Query: 258 AAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQFRFLDKAAIKDEEIVG 317
             V VA+ RK ++ +P D S LY+ +DA + +    +KL  +K   F DKAA++ E    
Sbjct: 255 LQVIVASCRKSKVKIPEDESLLYENQDA-ESSIIGSRKLEHTKILTFFDKAAVETESDNK 314

Query: 318 NVV--NKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTMNRHIGKS 377
                + W L T+T +EE+K ++R+LPIWAT I+F +VY+QM T  V Q  T+++H+G +
Sbjct: 315 GAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPN 374

Query: 378 FQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQRVGVGLVLSIIAMVA 437
           F+IP+ASL+ F   S+L   P+YD+LIVP A +   + +G T LQR+G+GLV+SI +MV+
Sbjct: 375 FKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVS 434

Query: 438 AALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALTYMGQLDFFLRECPK 497
           A + E+ RL  V+ + L  +    +PM++FW  PQ+ +VG  E  T++GQL+FF  + P 
Sbjct: 435 AGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPD 494

Query: 498 GMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENG--HPWIPDNLNEGKLYDFYWLLAIL 557
            M+++ + L L+ +A G + S+ LVT+V KVT +G    WI  NLN G L  F+WLLA L
Sbjct: 495 AMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLAGL 554

Query: 558 SVLNLAVFLICAKWYVYKE 573
           S LN  V+L  AKWY YK+
Sbjct: 555 SFLNFLVYLWIAKWYTYKK 564

BLAST of Carg27916 vs. ExPASy TrEMBL
Match: A0A6J1E3S2 (protein NRT1/ PTR FAMILY 6.3-like OS=Cucurbita moschata OX=3662 GN=LOC111430321 PE=3 SV=1)

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 588/590 (99.66%), Postives = 589/590 (99.83%), Query Frame = 0

Query: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60
           MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL
Sbjct: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60

Query: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120
           TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS
Sbjct: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120

Query: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180
           TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDE 
Sbjct: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDEA 180

Query: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240
           DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG
Sbjct: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240

Query: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300
           TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK
Sbjct: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300

Query: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360
           QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF
Sbjct: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360

Query: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQ 420
           SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYD+LIVPIASRILKNPQGLTPLQ
Sbjct: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDRLIVPIASRILKNPQGLTPLQ 420

Query: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480
           RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL
Sbjct: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480

Query: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540
           TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE
Sbjct: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540

Query: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 591
           GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH
Sbjct: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 590

BLAST of Carg27916 vs. ExPASy TrEMBL
Match: A0A6J1I186 (protein NRT1/ PTR FAMILY 6.3-like OS=Cucurbita maxima OX=3661 GN=LOC111468952 PE=3 SV=1)

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 580/590 (98.31%), Postives = 588/590 (99.66%), Query Frame = 0

Query: 1   MSILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60
           MSILP+TTHEKT+SDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL
Sbjct: 1   MSILPQTTHEKTVSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYL 60

Query: 61  TETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIS 120
           TETMHLGNATAANNVTNFLGTSFMLCL GGFVADTFLGRYLTISIFV VQAIGVTLLTIS
Sbjct: 61  TETMHLGNATAANNVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFVIVQAIGVTLLTIS 120

Query: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDET 180
           TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFD+T
Sbjct: 121 TIIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDDT 180

Query: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240
           DKEERAKTAVFFNWFFFLISIGSLGAVT+LVYIQDNWGRQWGYGICACAIVLGLVVFLSG
Sbjct: 181 DKEERAKTAVFFNWFFFLISIGSLGAVTLLVYIQDNWGRQWGYGICACAIVLGLVVFLSG 240

Query: 241 TRRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300
           TRRYRFKK+VGSPLTQIAAVFVAAWRKRR+DLPSDPSALYQIEDAVDGAGKKKQKLPRSK
Sbjct: 241 TRRYRFKKMVGSPLTQIAAVFVAAWRKRRMDLPSDPSALYQIEDAVDGAGKKKQKLPRSK 300

Query: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360
           QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF
Sbjct: 301 QFRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTF 360

Query: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQ 420
           SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYD+LIVPIASRILKNPQGLTPLQ
Sbjct: 361 SVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDRLIVPIASRILKNPQGLTPLQ 420

Query: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEAL 480
           RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLV+KPMAEVPMSVFWLCPQFLIVGAGEAL
Sbjct: 421 RVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVYKPMAEVPMSVFWLCPQFLIVGAGEAL 480

Query: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540
           TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE
Sbjct: 481 TYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNE 540

Query: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 591
           GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH
Sbjct: 541 GKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 590

BLAST of Carg27916 vs. ExPASy TrEMBL
Match: A0A0A0KMV3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G161290 PE=3 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 2.6e-309
Identity = 535/589 (90.83%), Postives = 564/589 (95.76%), Query Frame = 0

Query: 2   SILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLT 61
           S+LPET  EKTL DAWDYTGKPAIKAKTGGWVAAAMILGPE VERLTTLGIAVNLVTYLT
Sbjct: 3   SVLPETIQEKTLPDAWDYTGKPAIKAKTGGWVAAAMILGPEAVERLTTLGIAVNLVTYLT 62

Query: 62  ETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIST 121
           ETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIF TVQA GVT+LTIST
Sbjct: 63  ETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAAGVTILTIST 122

Query: 122 IIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETD 181
           IIPSLRPPKCSPG+S TPCI ADSKQLA+LHTALYLTALGTGGLKSSVSGFGSDQFD++D
Sbjct: 123 IIPSLRPPKCSPGVSTTPCIMADSKQLAVLHTALYLTALGTGGLKSSVSGFGSDQFDDSD 182

Query: 182 KEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGT 241
           KEERAK + FFNWFFF+ISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLV FL GT
Sbjct: 183 KEERAKMSKFFNWFFFIISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVAFLLGT 242

Query: 242 RRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQ 301
           R+YRFKKLVGSPLTQI AVF AAWRKR+LDLPSDP +LYQIEDAVDG+GKKKQKLPR+KQ
Sbjct: 243 RKYRFKKLVGSPLTQIVAVFYAAWRKRKLDLPSDPFSLYQIEDAVDGSGKKKQKLPRTKQ 302

Query: 302 FRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS 361
           FRFLDKAAIKDEE+VGNVVNKW +STLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS
Sbjct: 303 FRFLDKAAIKDEEVVGNVVNKWKISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS 362

Query: 362 VSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQR 421
           VSQATTMNRH+GKSF+IPAASLT FFVASIL+TVPIYD+ I+PIASRILKNPQGL+PLQR
Sbjct: 363 VSQATTMNRHMGKSFEIPAASLTVFFVASILLTVPIYDRFIIPIASRILKNPQGLSPLQR 422

Query: 422 VGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALT 481
           VGVGLVLSIIAMVAAALTEIKRL+VVEENGL +KP AEVP+SVFWL PQFL+VG+GEA T
Sbjct: 423 VGVGLVLSIIAMVAAALTEIKRLKVVEENGLTYKPTAEVPLSVFWLAPQFLLVGSGEAFT 482

Query: 482 YMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNEG 541
           YMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTE+G PWIPDNLNEG
Sbjct: 483 YMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTEHGRPWIPDNLNEG 542

Query: 542 KLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 591
           KLYDFYWLLAILSVLNL VFL+CAKWYVYKEKRLADEGIELEDCGP+ H
Sbjct: 543 KLYDFYWLLAILSVLNLMVFLVCAKWYVYKEKRLADEGIELEDCGPTVH 591

BLAST of Carg27916 vs. ExPASy TrEMBL
Match: A0A5A7T897 (Protein NRT1/ PTR FAMILY 6.3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00250 PE=3 SV=1)

HSP 1 Score: 1066.6 bits (2757), Expect = 3.7e-308
Identity = 533/589 (90.49%), Postives = 564/589 (95.76%), Query Frame = 0

Query: 2   SILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLT 61
           S+LPET  EKTL DAWDYTGKPAIK+KTGGWVAAAMILGPE VERLTTLGIAVNLVTYLT
Sbjct: 3   SVLPETIQEKTLPDAWDYTGKPAIKSKTGGWVAAAMILGPEAVERLTTLGIAVNLVTYLT 62

Query: 62  ETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIST 121
           ETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIF TVQA GVTLLTIST
Sbjct: 63  ETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQATGVTLLTIST 122

Query: 122 IIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETD 181
           IIPSLRPPKCSPG+S TPCI ADSKQLA+LHTALYLTALGTGGLKSSVSGFGSDQFD++D
Sbjct: 123 IIPSLRPPKCSPGVSTTPCIMADSKQLAVLHTALYLTALGTGGLKSSVSGFGSDQFDDSD 182

Query: 182 KEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGT 241
           KEER K + FFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIV+GLVVFL GT
Sbjct: 183 KEERVKMSNFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVMGLVVFLLGT 242

Query: 242 RRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQ 301
           R+YRFKKLVGSPLTQIAAV  AAWRKR+LDLPSDP +LYQIEDAVDG+GKKKQKLPR+KQ
Sbjct: 243 RKYRFKKLVGSPLTQIAAVIYAAWRKRKLDLPSDPFSLYQIEDAVDGSGKKKQKLPRTKQ 302

Query: 302 FRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS 361
           FRFLDKAAIKDEE+VGNVVNKW +STLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS
Sbjct: 303 FRFLDKAAIKDEEVVGNVVNKWKISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS 362

Query: 362 VSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQR 421
           VSQA+TMNRH+GKSF+IPAASLT FFVASIL+TVPIYD+ IVPIASRILKNPQGL+PLQR
Sbjct: 363 VSQASTMNRHMGKSFEIPAASLTVFFVASILLTVPIYDRFIVPIASRILKNPQGLSPLQR 422

Query: 422 VGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALT 481
           VGVGLVLSIIAM+AAALTEIKRL+VVEENGL +KP AEVP+SVFWL PQFL+VG+GEA T
Sbjct: 423 VGVGLVLSIIAMIAAALTEIKRLKVVEENGLTYKPTAEVPLSVFWLAPQFLLVGSGEAFT 482

Query: 482 YMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNEG 541
           YMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTE+G PWIPDNLNEG
Sbjct: 483 YMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTEHGKPWIPDNLNEG 542

Query: 542 KLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 591
           KLYDFYWLLA+LSVLNL VFL+CAKWYVYKEKRLADEGIELEDCGP+ H
Sbjct: 543 KLYDFYWLLAVLSVLNLMVFLVCAKWYVYKEKRLADEGIELEDCGPTVH 591

BLAST of Carg27916 vs. ExPASy TrEMBL
Match: A0A1S3ATS1 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 6.3-like OS=Cucumis melo OX=3656 GN=LOC103482632 PE=3 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 2.4e-307
Identity = 532/589 (90.32%), Postives = 563/589 (95.59%), Query Frame = 0

Query: 2   SILPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLT 61
           S+LPET  EKTL DAWDYTGKPAIK+KTGGWVAAAMILGPE VERLTTLGIAVNLVTYLT
Sbjct: 3   SVLPETIQEKTLPDAWDYTGKPAIKSKTGGWVAAAMILGPEAVERLTTLGIAVNLVTYLT 62

Query: 62  ETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTIST 121
           ETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIF TVQA GVTLLTIST
Sbjct: 63  ETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQATGVTLLTIST 122

Query: 122 IIPSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETD 181
           IIPSLRPPKCSPG+S TPCI ADSKQLA+LHTALYLTALGTGGLKSSVSGFGSDQFD++D
Sbjct: 123 IIPSLRPPKCSPGVSTTPCIMADSKQLAVLHTALYLTALGTGGLKSSVSGFGSDQFDDSD 182

Query: 182 KEERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGT 241
           KEER K + FFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIV+GLVVFL GT
Sbjct: 183 KEERVKMSNFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVMGLVVFLLGT 242

Query: 242 RRYRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQ 301
           R+YRFKKLVGSPLTQIAAV  AAWRKR+LDLPSDP +LYQIEDAVDG+GKKKQKLPR+KQ
Sbjct: 243 RKYRFKKLVGSPLTQIAAVIYAAWRKRKLDLPSDPFSLYQIEDAVDGSGKKKQKLPRTKQ 302

Query: 302 FRFLDKAAIKDEEIVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS 361
           FRFLDKAAIKDEE+VGNVVNKW +STLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS
Sbjct: 303 FRFLDKAAIKDEEVVGNVVNKWKISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS 362

Query: 362 VSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQR 421
           VSQA+TMNRH+GKSF+IPAASLT FFVASIL+TVPIYD+ IVPIASRILKNPQGL+PLQR
Sbjct: 363 VSQASTMNRHMGKSFEIPAASLTVFFVASILLTVPIYDRFIVPIASRILKNPQGLSPLQR 422

Query: 422 VGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALT 481
           VGVGLVLSIIAM+AAALTEIKRL+VVEENGL +KP AEVP+SVFWL PQFL+VG+GEA T
Sbjct: 423 VGVGLVLSIIAMIAAALTEIKRLKVVEENGLTYKPTAEVPLSVFWLAPQFLLVGSGEAFT 482

Query: 482 YMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNEG 541
           YMGQLDFFLRECPKGMKTMSTGLFLSTLALG FFSSLLVTIVGKVTE+G PWIPDNLNEG
Sbjct: 483 YMGQLDFFLRECPKGMKTMSTGLFLSTLALGXFFSSLLVTIVGKVTEHGKPWIPDNLNEG 542

Query: 542 KLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELEDCGPSAH 591
           KLYDFYWLLA+LSVLNL VFL+CAKWYVYKEKRLADEGIELEDCGP+ H
Sbjct: 543 KLYDFYWLLAVLSVLNLMVFLVCAKWYVYKEKRLADEGIELEDCGPTVH 591

BLAST of Carg27916 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 746.1 bits (1925), Expect = 2.1e-215
Identity = 378/585 (64.62%), Postives = 465/585 (79.49%), Query Frame = 0

Query: 4   LPETTHEKTLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTET 63
           LPET  +  L DAWD+ G+PA ++KTGGW +AAMIL  E VERLTTLGI VNLVTYLT T
Sbjct: 3   LPETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGT 62

Query: 64  MHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTII 123
           MHLGNATAAN VTNFLGTSFMLCL GGF+ADTFLGRYLTI+IF  +QA GV++LT+STII
Sbjct: 63  MHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTII 122

Query: 124 PSLRPPKCSPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKE 183
           P LRPP+C+P  S + C  A   QL +L+ ALYLTALGTGG+K+SVSGFGSDQFDET+ +
Sbjct: 123 PGLRPPRCNPTTS-SHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPK 182

Query: 184 ERAKTAVFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRR 243
           ER+K   FFN FFF I++GSL AVT+LVY+QD+ GR+WGYGICA AIVL L VFL+GT R
Sbjct: 183 ERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNR 242

Query: 244 YRFKKLVGSPLTQIAAVFVAAWRKRRLDLPSDPSALYQIED--AVDGAGKKKQKLPRSKQ 303
           YRFKKL+GSP+TQ+AAV VAAWR R+L+LP+DPS LY ++D  A +G+ K KQKLP ++Q
Sbjct: 243 YRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQ 302

Query: 304 FRFLDKAAIKDEE--IVGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTT 363
           FR LDKAAI+D+E  +  NV NKW LSTLTD+EEVK ++RMLPIWAT I+FWTV+AQ+TT
Sbjct: 303 FRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTT 362

Query: 364 FSVSQATTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPL 423
            SV+Q+ T++R IG SF+IP AS+  F+V  +L+T  +YD++ + +  ++   P GL PL
Sbjct: 363 LSVAQSETLDRSIG-SFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPL 422

Query: 424 QRVGVGLVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEA 483
           QR+G+GL    +AM  AAL E+KRLR    +G   K +   P+  + L PQ+LIVG GEA
Sbjct: 423 QRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTL---PLGFYLLIPQYLIVGIGEA 482

Query: 484 LTYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLN 543
           L Y GQLDFFLRECPKGMK MSTGL LSTLALGFFFSS+LVTIV K T   HPWI D+LN
Sbjct: 483 LIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLN 542

Query: 544 EGKLYDFYWLLAILSVLNLAVFLICAKWYVYKEKRLADEGIELED 585
           +G+LY+FYWL+A+L  LN  +FL+ +KWYVYKEKRLA+ GIEL+D
Sbjct: 543 KGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDD 582

BLAST of Carg27916 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 595.5 bits (1534), Expect = 4.6e-170
Identity = 325/590 (55.08%), Postives = 420/590 (71.19%), Query Frame = 0

Query: 12  TLSDAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTETMHLGNATA 71
           T++DA DY G+PA K+KTGGW+ AA+ILG E VERL+T+GIAVNLVTYL ETMHL ++T+
Sbjct: 8   TVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTS 67

Query: 72  ANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTIIPSLRPPKC 131
           AN VT+F+GTSF+LCL GGF+AD+FLGR+ TI IF T+QA+G   L ++T +P LRPP C
Sbjct: 68  ANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTC 127

Query: 132 SPGLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKEERAKTAVF 191
             G     CIPA + Q+ IL+ +LYL ALGTGGLKSS+SGFGSDQFD+ D +E+A  A F
Sbjct: 128 HHG---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFF 187

Query: 192 FNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRRYRFKKLVG 251
           FN FFF IS+G+L AVT+LVY+QD  GR W YGIC  ++ + +V+FL GT+RYR+KK  G
Sbjct: 188 FNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQG 247

Query: 252 SPLTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQFRFLDKAAIK 311
           SP+ QI  V  AA+RKR+++LP   S +Y  ED  +G      ++  + QF  LDKAAI 
Sbjct: 248 SPVVQIFQVIAAAFRKRKMELPQ--SIVYLYEDNPEGI-----RIEHTDQFHLLDKAAIV 307

Query: 312 -----DEEIVGNVV-NKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQA 371
                ++ + G  + N W LS++T +EEVK+++R+LPIWATTIIFWT YAQM TFSV QA
Sbjct: 308 AEGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQA 367

Query: 372 TTMNRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQRVGVG 431
           +TM R+IG SF+IPA SLT FFVA+IL+T+ +YD+ I+P   +  K   G + LQR+ +G
Sbjct: 368 STMRRNIG-SFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIG 427

Query: 432 LVLSIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALTYMGQ 491
           LVLS   M AAAL E KRL V + +         +P+SVF L PQF +VGAGEA  Y GQ
Sbjct: 428 LVLSTAGMAAAALVEQKRLSVAKSSS-----QKTLPISVFLLVPQFFLVGAGEAFIYTGQ 487

Query: 492 LDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHP--WIPDNLNEGKL 551
           LDFF+ + PKGMKTMSTGLFL+TL+LGFF SS LV+IV +VT       W+ DN+N G+L
Sbjct: 488 LDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRL 547

Query: 552 YDFYWLLAILSVLNLAVFLICAKWY--------VYKEKRLADEGIELEDC 586
             FYWLL ILS +N  V++ICA W+        V KE     +G  +EDC
Sbjct: 548 DYFYWLLVILSGINFVVYIICALWFKPTKGKDSVEKEN---GKGFSVEDC 577

BLAST of Carg27916 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 504.2 bits (1297), Expect = 1.4e-142
Identity = 275/563 (48.85%), Postives = 374/563 (66.43%), Query Frame = 0

Query: 15  DAWDYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTETMHLGNATAANN 74
           +A+DY G P  K+KTGGW+ A +ILG E  ER+  +GI++NLVTYL   +H+ +A +A  
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 75  VTNFLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTIIPSLRPPKCSP- 134
           VTNF+GT  +L L GGF+AD  LGRY  ++I  +V A+GV LLT++T I S+RPP C   
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 135 GLSPTPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKEERAKTAVFFN 194
                 CI A+  QLA+L+ ALY  ALG GG+KS+VSGFGSDQFD +D +E  +   FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 195 WFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRRYRFKKLVGSP 254
            F+F IS+GSL AV  LVY+QDN GR WGYGI A  +V+  +V L GT+RYRFKK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 255 LTQIAAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQFRFLDKAAIKDE 314
            T I  V   AW+KR+   P+ PS L          G     +P ++  + LDKAAI   
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL---------NGYDNTTVPHTEMLKCLDKAAISKN 315

Query: 315 EIVGN-----VVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTM 374
           E   +       + W +ST+T +EEVKLV++++PIWAT I+FWT+Y+QMTTF+V QAT M
Sbjct: 316 ESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFM 375

Query: 375 NRHIGKSFQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQRVGVGLVL 434
           +R +G SF +PA S +AF + +IL+   + +++ VP+  R+ K PQG+T LQR+GVGLV 
Sbjct: 376 DRKLG-SFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVF 435

Query: 435 SIIAMVAAALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALTYMGQLDF 494
           S+ AM  AA+ E  R      N        +  +S FWL PQ+ +VGAGEA  Y+GQL+F
Sbjct: 436 SMAAMAVAAVIENARREAAVNN--------DKKISAFWLVPQYFLVGAGEAFAYVGQLEF 495

Query: 495 FLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENGHPWIPDNLNEGKLYDFYW 554
           F+RE P+ MK+MSTGLFLST+++GFF SSLLV++V +VT+    W+  NLN+ +L  FYW
Sbjct: 496 FIREAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKS--WLRSNLNKARLNYFYW 555

Query: 555 LLAILSVLNLAVFLICAKWYVYK 572
           LL +L  LN  +F++ A  + YK
Sbjct: 556 LLVVLGALNFLIFIVFAMKHQYK 558

BLAST of Carg27916 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 475.7 bits (1223), Expect = 5.4e-134
Identity = 257/559 (45.97%), Postives = 366/559 (65.47%), Query Frame = 0

Query: 18  DYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTETMHLGNATAANNVTN 77
           D    PA K KTG W A   ILG E  ERL   G+  NLV YL   ++ GNATAANNVTN
Sbjct: 14  DIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTN 73

Query: 78  FLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTIIPSLRPPKCSPGLSP 137
           + GT ++  L G F+AD +LGRY TI+ FV +   G+TLLT+S  +P L+P  C    + 
Sbjct: 74  WSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNC----NA 133

Query: 138 TPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKEERAKTAVFFNWFFF 197
             C P +S Q A+   ALY+ ALGTGG+K  VS FG+DQFDE D+ E+ K + FFNWF+F
Sbjct: 134 DTCHP-NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYF 193

Query: 198 LISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRRYRFKKLVGSPLTQI 257
            I++G+L A T+LV+IQ N G  WG+G+   A+V+ +  F  G+R YR ++  GSPLT+I
Sbjct: 194 SINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRI 253

Query: 258 AAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQFRFLDKAAI--KDEEI 317
             V VAA+RK  + +P D S L++  D      K  +KL  +   +F DKAA+  + + I
Sbjct: 254 FQVIVAAFRKISVKVPEDKSLLFETADDESNI-KGSRKLVHTDNLKFFDKAAVESQSDSI 313

Query: 318 VGNVVNKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTMNRHIGKS 377
               VN W L ++T +EE+K ++ +LP+WAT I+F TVY+QM+T  V Q  TM++H+GK+
Sbjct: 314 KDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKN 373

Query: 378 FQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQRVGVGLVLSIIAMVA 437
           F+IP+ASL+ F   S+L   P+YDQ I+P+A +  +N +G T LQR+G+GLV+SI AM+ 
Sbjct: 374 FEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMIT 433

Query: 438 AALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALTYMGQLDFFLRECPK 497
           A + E+ RL  V+ +        ++ MS+FW  PQ+L++G  E  T++GQL+FF  + P 
Sbjct: 434 AGVLEVVRLDYVKTHNAY--DQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPD 493

Query: 498 GMKTMSTGLFLSTLALGFFFSSLLVTIVGKVT-ENGHP-WIPDNLNEGKLYDFYWLLAIL 557
            M+++ + L L+T+ALG + S++LVT+V K+T +NG P WIPDNLN G L  F++LLA L
Sbjct: 494 AMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATL 553

Query: 558 SVLNLAVFLICAKWYVYKE 573
           S LN  V+L  +K Y YK+
Sbjct: 554 SFLNFLVYLWISKRYKYKK 564

BLAST of Carg27916 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 448.7 bits (1153), Expect = 7.0e-126
Identity = 247/559 (44.19%), Postives = 360/559 (64.40%), Query Frame = 0

Query: 18  DYTGKPAIKAKTGGWVAAAMILGPEGVERLTTLGIAVNLVTYLTETMHLGNATAANNVTN 77
           D   KPA K KTG W A   ILG E  ERL   G++ NL+ YL + M++ N +A+ +V+N
Sbjct: 15  DIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSN 74

Query: 78  FLGTSFMLCLFGGFVADTFLGRYLTISIFVTVQAIGVTLLTISTIIPSLRPPKCSPGLSP 137
           + GT +   L G F+AD +LGRY TI+ FV +   G+TLLTIS  +P L     +P  S 
Sbjct: 75  WSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-----TPTCSG 134

Query: 138 TPCIPADSKQLAILHTALYLTALGTGGLKSSVSGFGSDQFDETDKEERAKTAVFFNWFFF 197
             C  A + Q AI   ALYL ALGTGG+K  VS FG+DQFD+TD++E+   + FFNWF+F
Sbjct: 135 ETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYF 194

Query: 198 LISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVVFLSGTRRYRFKKLVGSPLTQI 257
           +I++G++ A ++LV+IQ N G  WG G+   A+ + +V F +G+  YR +K  GSPLT++
Sbjct: 195 VINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRM 254

Query: 258 AAVFVAAWRKRRLDLPSDPSALYQIEDAVDGAGKKKQKLPRSKQFRFLDKAAIKDEEIVG 317
             V VA+ RK ++ +P D S LY+ +DA + +    +KL  +K   F DKAA++ E    
Sbjct: 255 LQVIVASCRKSKVKIPEDESLLYENQDA-ESSIIGSRKLEHTKILTFFDKAAVETESDNK 314

Query: 318 NVV--NKWNLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTMNRHIGKS 377
                + W L T+T +EE+K ++R+LPIWAT I+F +VY+QM T  V Q  T+++H+G +
Sbjct: 315 GAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPN 374

Query: 378 FQIPAASLTAFFVASILVTVPIYDQLIVPIASRILKNPQGLTPLQRVGVGLVLSIIAMVA 437
           F+IP+ASL+ F   S+L   P+YD+LIVP A +   + +G T LQR+G+GLV+SI +MV+
Sbjct: 375 FKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVS 434

Query: 438 AALTEIKRLRVVEENGLVFKPMAEVPMSVFWLCPQFLIVGAGEALTYMGQLDFFLRECPK 497
           A + E+ RL  V+ + L  +    +PM++FW  PQ+ +VG  E  T++GQL+FF  + P 
Sbjct: 435 AGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPD 494

Query: 498 GMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTENG--HPWIPDNLNEGKLYDFYWLLAIL 557
            M+++ + L L+ +A G + S+ LVT+V KVT +G    WI  NLN G L  F+WLLA L
Sbjct: 495 AMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLAGL 554

Query: 558 SVLNLAVFLICAKWYVYKE 573
           S LN  V+L  AKWY YK+
Sbjct: 555 SFLNFLVYLWIAKWYTYKK 564

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7016898.10.0e+00100.00Protein NRT1/ PTR FAMILY 6.3, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
KAG6579420.10.0e+0099.66Protein NRT1/ PTR FAMILY 6.3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022922301.10.0e+0099.66protein NRT1/ PTR FAMILY 6.3-like [Cucurbita moschata][more]
XP_022969890.10.0e+0098.31protein NRT1/ PTR FAMILY 6.3-like [Cucurbita maxima][more]
XP_023550325.10.0e+0098.98protein NRT1/ PTR FAMILY 6.3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q050853.0e-21464.62Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9SZY46.5e-16955.08Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q9LVE02.0e-14148.85Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q9M3907.6e-13345.97Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB89.9e-12544.19Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E3S20.0e+0099.66protein NRT1/ PTR FAMILY 6.3-like OS=Cucurbita moschata OX=3662 GN=LOC111430321 ... [more]
A0A6J1I1860.0e+0098.31protein NRT1/ PTR FAMILY 6.3-like OS=Cucurbita maxima OX=3661 GN=LOC111468952 PE... [more]
A0A0A0KMV32.6e-30990.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G161290 PE=3 SV=1[more]
A0A5A7T8973.7e-30890.49Protein NRT1/ PTR FAMILY 6.3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A1S3ATS12.4e-30790.32LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 6.3-like OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
AT1G12110.12.1e-21564.62nitrate transporter 1.1 [more]
AT2G26690.14.6e-17055.08Major facilitator superfamily protein [more]
AT3G21670.11.4e-14248.85Major facilitator superfamily protein [more]
AT3G54140.15.4e-13445.97peptide transporter 1 [more]
AT5G01180.17.0e-12644.19peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 100..534
e-value: 2.8E-116
score: 388.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 11..579
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 3..578
e-value: 8.6E-202
score: 673.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 32..571
NoneNo IPR availablePANTHERPTHR11654:SF507PROTEIN NRT1/ PTR FAMILY 6.3coord: 11..579
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 89..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27916-RACarg27916-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015706 nitrate transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0010167 response to nitrate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity