Carg27771 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27771
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	hydrophobic protein LTI6B-like
Location	Carg_Chr10: 10688773 .. 10689323 (-)
RNA-Seq Expression	Carg27771
Synteny	Carg27771

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCGCTACCCACTACCACTGCTTCTAACGCCTCCTCCATTCCCCTCTGTTTGAATCCGAAGAAATTTCATAGCAAAACAATGGCCGACCCGGCGACCGCCAACTGCATCGATATCCTTCTGGCTATTATCTCCTGCCGCCGTCTCGGAGTGTTTCTCAAATTTGGATGCCAGGTCTGTGTCGCTCTCATCTTTTCTTCTTATACTTGGTTAATCGATGTTGTTGATTTGATGAACATGTAATAAACCATATTTGGTGTTCCATAGGTTGAGTTTTGGATCTGTTTGGTGCTAACTTGTTCGAATATCTCCCCGGGACATCTTGCTGTTTATGCTATCACCAAGTGATTCCGAGATGTGCTTTCATCATCAAAACTGTGTGTGTTTAGTTGGTTTTGTGCTATGTGTTTGTGTGCTGTGCTTCGTTTCTAAATTCTTTTTTCTTGTGTTTTGTGGCTGTTGTTGTTGTTGGTTGTATTGATTGAATTGGTTGCTGTAAACAAAATCCATCCTACTTTTTCTTCAACTTAATTACAACTTTACTTATATTTT

mRNA sequence

CATCGCTACCCACTACCACTGCTTCTAACGCCTCCTCCATTCCCCTCTGTTTGAATCCGAAGAAATTTCATAGCAAAACAATGGCCGACCCGGCGACCGCCAACTGCATCGATATCCTTCTGGCTATTATCTCCTGCCGCCGTCTCGGAGTGTTTCTCAAATTTGGATGCCAGGTTGAGTTTTGGATCTGTTTGGTGCTAACTTGTTCGAATATCTCCCCGGGACATCTTGCTGTTTATGCTATCACCAAGTGATTCCGAGATGTGCTTTCATCATCAAAACTGTGTGTGTTTAGTTGGTTTTGTGCTATGTGTTTGTGTGCTGTGCTTCGTTTCTAAATTCTTTTTTCTTGTGTTTTGTGGCTGTTGTTGTTGTTGGTTGTATTGATTGAATTGGTTGCTGTAAACAAAATCCATCCTACTTTTTCTTCAACTTAATTACAACTTTACTTATATTTT

Coding sequence (CDS)

ATGGCCGACCCGGCGACCGCCAACTGCATCGATATCCTTCTGGCTATTATCTCCTGCCGCCGTCTCGGAGTGTTTCTCAAATTTGGATGCCAGGTTGAGTTTTGGATCTGTTTGGTGCTAACTTGTTCGAATATCTCCCCGGGACATCTTGCTGTTTATGCTATCACCAAGTGA

Protein sequence

MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHLAVYAITK

Homology

[Close]

BLAST of Carg27771 vs. NCBI nr
Match: KAG7024052.1 (Hydrophobic protein LTI6B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 121.3 bits (303), Expect = 2.7e-24
Identity = 57/57 (100.00%), Postives = 57/57 (100.00%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHLAVYAITK 58
          MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHLAVYAITK
Sbjct: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHLAVYAITK 57

[Close]

BLAST of Carg27771 vs. NCBI nr
Match: XP_022961459.1 (hydrophobic protein LTI6B-like [Cucurbita moschata] >XP_022968842.1 hydrophobic protein LTI6B-like [Cucurbita maxima] >XP_023539025.1 hydrophobic protein LTI6B-like [Cucurbita pepo subsp. pepo] >KAG7024051.1 Hydrophobic protein LTI6B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 80.9 bits (198), Expect = 4.0e-12
Identity = 44/58 (75.86%), Postives = 46/58 (79.31%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MADP TANCIDILLAI+    LGVFLKFGCQVEFWICLVLT     PG + AVYAITK
Sbjct: 1  MADPGTANCIDILLAIL-LPPLGVFLKFGCQVEFWICLVLTLFGYLPGIIYAVYAITK 57

[Close]

BLAST of Carg27771 vs. NCBI nr
Match: KAF7818031.1 (hydrophobic protein RCI2B [Senna tora])

HSP 1 Score: 79.0 bits (193), Expect = 1.5e-11
Identity = 43/58 (74.14%), Postives = 46/58 (79.31%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MADP TANCIDILLAII    LGVFLKFGC+VEFWICL+LT     PG + AVYAITK
Sbjct: 1  MADPGTANCIDILLAII-LPPLGVFLKFGCKVEFWICLLLTIFGYLPGIIYAVYAITK 57

[Close]

BLAST of Carg27771 vs. NCBI nr
Match: RWW57479.1 (hypothetical protein BHE74_00035732 [Ensete ventricosum])

HSP 1 Score: 77.4 bits (189), Expect = 4.4e-11
Identity = 43/58 (74.14%), Postives = 45/58 (77.59%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MAD  TANCIDILLAII    LGVFLKFGCQVEFWICL+LT     PG + AVYAITK
Sbjct: 1  MADEGTANCIDILLAII-LPPLGVFLKFGCQVEFWICLLLTLFGYLPGIIYAVYAITK 57

[Close]

BLAST of Carg27771 vs. NCBI nr
Match: XP_038879764.1 (hydrophobic protein LTI6B-like [Benincasa hispida])

HSP 1 Score: 77.4 bits (189), Expect = 4.4e-11
Identity = 43/58 (74.14%), Postives = 45/58 (77.59%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MAD AT NCIDILLAI+    LGVFLKFGCQVEFWICLVLT     PG + AVYAITK
Sbjct: 37 MADEATTNCIDILLAIL-LPPLGVFLKFGCQVEFWICLVLTFFGYIPGIIYAVYAITK 93

[Close]

BLAST of Carg27771 vs. ExPASy Swiss-Prot
Match: A2Y075 (Hydrophobic protein LTI6B OS=Oryza sativa subsp. indica OX=39946 GN=LTI6B PE=3 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 3.9e-10
Identity = 36/53 (67.92%), Postives = 40/53 (75.47%), Query Frame = 0


Query: 6  TANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          TANCIDIL+AII    LGVFLKFGC  EFWICL+LT     PG + A+YAITK
Sbjct: 4  TANCIDILIAII-LPPLGVFLKFGCGHEFWICLLLTFLGYIPGIIYAIYAITK 55

[Close]

BLAST of Carg27771 vs. ExPASy Swiss-Prot
Match: Q0DKW8 (Hydrophobic protein LTI6B OS=Oryza sativa subsp. japonica OX=39947 GN=LTI6B PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 3.9e-10
Identity = 36/53 (67.92%), Postives = 40/53 (75.47%), Query Frame = 0


Query: 6  TANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          TANCIDIL+AII    LGVFLKFGC  EFWICL+LT     PG + A+YAITK
Sbjct: 4  TANCIDILIAII-LPPLGVFLKFGCGHEFWICLLLTFLGYIPGIIYAIYAITK 55

[Close]

BLAST of Carg27771 vs. ExPASy Swiss-Prot
Match: Q8H5T6 (Hydrophobic protein LTI6A OS=Oryza sativa subsp. japonica OX=39947 GN=LTI6A PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 3.3e-09
Identity = 36/58 (62.07%), Postives = 42/58 (72.41%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MAD +TA CIDI+LAII    LGVF KFGC +EFWICL+LT     PG + AV+ ITK
Sbjct: 1  MAD-STATCIDIILAII-LPPLGVFFKFGCGIEFWICLLLTFFGYLPGIIYAVWVITK 56

[Close]

BLAST of Carg27771 vs. ExPASy Swiss-Prot
Match: Q9ZNS6 (Hydrophobic protein RCI2B OS=Arabidopsis thaliana OX=3702 GN=RCI2B PE=2 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.2e-08
Identity = 33/54 (61.11%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 5  ATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          +TA  ++I+LAII    LGVFLKFGC+VEFWICL+LT     PG L A+Y ITK
Sbjct: 2  STATFVEIILAII-LPPLGVFLKFGCKVEFWICLILTLFGYLPGILYALYIITK 54

[Close]

BLAST of Carg27771 vs. ExPASy Swiss-Prot
Match: Q9ZNQ7 (Hydrophobic protein RCI2A OS=Arabidopsis thaliana OX=3702 GN=RCI2A PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 6.2e-08
Identity = 30/54 (55.56%), Postives = 39/54 (72.22%), Query Frame = 0


Query: 5  ATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          +TA  +DI++AI+    LGVFL+FGC VEFWICLVLT     PG + A+Y +TK
Sbjct: 2  STATFVDIIIAIL-LPPLGVFLRFGCGVEFWICLVLTLLGYIPGIIYAIYVLTK 54

[Close]

BLAST of Carg27771 vs. ExPASy TrEMBL
Match: A0A6J1HUN0 (hydrophobic protein LTI6B-like OS=Cucurbita maxima OX=3661 GN=LOC111467977 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-12
Identity = 44/58 (75.86%), Postives = 46/58 (79.31%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MADP TANCIDILLAI+    LGVFLKFGCQVEFWICLVLT     PG + AVYAITK
Sbjct: 1  MADPGTANCIDILLAIL-LPPLGVFLKFGCQVEFWICLVLTLFGYLPGIIYAVYAITK 57

[Close]

BLAST of Carg27771 vs. ExPASy TrEMBL
Match: A0A6J1HE36 (hydrophobic protein LTI6B-like OS=Cucurbita moschata OX=3662 GN=LOC111462034 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-12
Identity = 44/58 (75.86%), Postives = 46/58 (79.31%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MADP TANCIDILLAI+    LGVFLKFGCQVEFWICLVLT     PG + AVYAITK
Sbjct: 1  MADPGTANCIDILLAIL-LPPLGVFLKFGCQVEFWICLVLTLFGYLPGIIYAVYAITK 57

[Close]

BLAST of Carg27771 vs. ExPASy TrEMBL
Match: A0A2K3L766 (Hydrophobic protein LTI6A-like OS=Trifolium pratense OX=57577 GN=L195_g030288 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.6e-11
Identity = 43/58 (74.14%), Postives = 44/58 (75.86%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MAD  TANCIDILLAII    LGVFLKFGC VEFWICLVLT     PG + AVYAITK
Sbjct: 1  MADEGTANCIDILLAII-LPPLGVFLKFGCNVEFWICLVLTLFGYIPGIIYAVYAITK 57

[Close]

BLAST of Carg27771 vs. ExPASy TrEMBL
Match: A0A0B0P7E4 (Hydrophobic RCI2B-like protein OS=Gossypium arboreum OX=29729 GN=LOC108462904 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.8e-11
Identity = 42/58 (72.41%), Postives = 45/58 (77.59%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MAD +TA CIDILLAII    LGVFLKFGCQVEFWICLVLT     PG + A+YAITK
Sbjct: 1  MADDSTATCIDILLAII-LPPLGVFLKFGCQVEFWICLVLTLFGYIPGIIYAIYAITK 57

[Close]

BLAST of Carg27771 vs. ExPASy TrEMBL
Match: A0A5D2MMY2 (Uncharacterized protein OS=Gossypium tomentosum OX=34277 GN=ES332_A13G216300v1 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.8e-11
Identity = 42/58 (72.41%), Postives = 45/58 (77.59%), Query Frame = 0


Query: 1  MADPATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          MAD +TA CIDILLAII    LGVFLKFGCQVEFWICLVLT     PG + A+YAITK
Sbjct: 1  MADDSTATCIDILLAII-LPPLGVFLKFGCQVEFWICLVLTLFGYIPGIIYAIYAITK 57

[Close]

BLAST of Carg27771 vs. TAIR 10
Match: AT3G05890.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 59.7 bits (143), Expect = 8.9e-10
Identity = 33/54 (61.11%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 5  ATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          +TA  ++I+LAII    LGVFLKFGC+VEFWICL+LT     PG L A+Y ITK
Sbjct: 2  STATFVEIILAII-LPPLGVFLKFGCKVEFWICLILTLFGYLPGILYALYIITK 54

[Close]

BLAST of Carg27771 vs. TAIR 10
Match: AT3G05880.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 57.4 bits (137), Expect = 4.4e-09
Identity = 30/54 (55.56%), Postives = 39/54 (72.22%), Query Frame = 0


Query: 5  ATANCIDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          +TA  +DI++AI+    LGVFL+FGC VEFWICLVLT     PG + A+Y +TK
Sbjct: 2  STATFVDIIIAIL-LPPLGVFLRFGCGVEFWICLVLTLLGYIPGIIYAIYVLTK 54

[Close]

BLAST of Carg27771 vs. TAIR 10
Match: AT2G38905.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 45.8 bits (107), Expect = 1.3e-05
Identity = 24/46 (52.17%), Postives = 33/46 (71.74%), Query Frame = 0


Query: 10 IDILLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHLAVYAI 56
          ++I+LAI+    +GVFL++GC VEFWICL+LT     PG   +YAI
Sbjct: 7  LEIILAIL-LPPVGVFLRYGCGVEFWICLLLTILGYIPG--IIYAI 49

[Close]

BLAST of Carg27771 vs. TAIR 10
Match: AT1G57550.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 42.0 bits (97), Expect = 1.9e-04
Identity = 20/46 (43.48%), Postives = 30/46 (65.22%), Query Frame = 0


Query: 13 LLAIISCRRLGVFLKFGCQVEFWICLVLTCSNISPGHL-AVYAITK 58
          +L  I    +GVFL++G  +EFW+CL+LT     PG + A+Y +TK
Sbjct: 7  VLCAIFIPPVGVFLRYGLGLEFWVCLLLTLFAFIPGLIYAIYVLTK 52

[Close]

BLAST of Carg27771 vs. TAIR 10
Match: AT2G24040.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 40.0 bits (92), Expect = 7.3e-04
Identity = 24/49 (48.98%), Postives = 33/49 (67.35%), Query Frame = 0


Query: 9  CIDILLAIISCRRLGVFLKFG-CQVEFWICLVLTCSNISPGHL-AVYAI 56
          C +I +AI+    +GV L+ G C VEF+ICL+LTC    PG + A+YAI
Sbjct: 8  CCEIFIAIL-LPPVGVCLRHGCCTVEFFICLILTCLGYLPGIIYAIYAI 55

The following BLAST results are available for this feature:

BLAST of Carg27771 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024052.1	2.7e-24	100.00	Hydrophobic protein LTI6B [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022961459.1	4.0e-12	75.86	hydrophobic protein LTI6B-like [Cucurbita moschata] >XP_022968842.1 hydrophobic ...	[more]
KAF7818031.1	1.5e-11	74.14	hydrophobic protein RCI2B [Senna tora]	[more]
RWW57479.1	4.4e-11	74.14	hypothetical protein BHE74_00035732 [Ensete ventricosum]	[more]
XP_038879764.1	4.4e-11	74.14	hydrophobic protein LTI6B-like [Benincasa hispida]	[more]

BLAST of Carg27771 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A2Y075	3.9e-10	67.92	Hydrophobic protein LTI6B OS=Oryza sativa subsp. indica OX=39946 GN=LTI6B PE=3 S...	[more]
Q0DKW8	3.9e-10	67.92	Hydrophobic protein LTI6B OS=Oryza sativa subsp. japonica OX=39947 GN=LTI6B PE=2...	[more]
Q8H5T6	3.3e-09	62.07	Hydrophobic protein LTI6A OS=Oryza sativa subsp. japonica OX=39947 GN=LTI6A PE=2...	[more]
Q9ZNS6	1.2e-08	61.11	Hydrophobic protein RCI2B OS=Arabidopsis thaliana OX=3702 GN=RCI2B PE=2 SV=1	[more]
Q9ZNQ7	6.2e-08	55.56	Hydrophobic protein RCI2A OS=Arabidopsis thaliana OX=3702 GN=RCI2A PE=2 SV=1	[more]

BLAST of Carg27771 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HUN0	1.9e-12	75.86	hydrophobic protein LTI6B-like OS=Cucurbita maxima OX=3661 GN=LOC111467977 PE=3 ...	[more]
A0A6J1HE36	1.9e-12	75.86	hydrophobic protein LTI6B-like OS=Cucurbita moschata OX=3662 GN=LOC111462034 PE=...	[more]
A0A2K3L766	3.6e-11	74.14	Hydrophobic protein LTI6A-like OS=Trifolium pratense OX=57577 GN=L195_g030288 PE...	[more]
A0A0B0P7E4	4.8e-11	72.41	Hydrophobic RCI2B-like protein OS=Gossypium arboreum OX=29729 GN=LOC108462904 PE...	[more]
A0A5D2MMY2	4.8e-11	72.41	Uncharacterized protein OS=Gossypium tomentosum OX=34277 GN=ES332_A13G216300v1 P...	[more]

BLAST of Carg27771 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05890.1	8.9e-10	61.11	Low temperature and salt responsive protein family	[more]
AT3G05880.1	4.4e-09	55.56	Low temperature and salt responsive protein family	[more]
AT2G38905.1	1.3e-05	52.17	Low temperature and salt responsive protein family	[more]
AT1G57550.1	1.9e-04	43.48	Low temperature and salt responsive protein family	[more]
AT2G24040.1	7.3e-04	48.98	Low temperature and salt responsive protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000612	Proteolipid membrane potential modulator	PFAM	PF01679	Pmp3	coord: 9..55 e-value: 7.2E-7 score: 29.3
IPR000612	Proteolipid membrane potential modulator	PANTHER	PTHR21659	HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED	coord: 1..57
None	No IPR available	PANTHER	PTHR21659:SF81	LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN	coord: 1..57

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27771-RA	Carg27771-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane