Carg27692 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27692
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	(1->3)-beta-glucan endohydrolase
Location	Carg_Chr13: 3835834 .. 3837793 (+)
RNA-Seq Expression	Carg27692
Synteny	Carg27692

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAGACGGAAAACCCTTCAGTTTTTTTTCTTTTTTTCCCCATCTTTTTCTATAATCAATCTGTTCAGATCACTTTCATTTGCTAATTCCATCTGGGTATCATTGCTTTTTGTTATTTCATTCTTTTTCCCCGTTTTGTTGGCTGTGAGCGAGGTGGGAAAATGGGAAGATTATGGATTCTGAAATGGGTTTTGTTGGTTGGGGTTTTGGGTTGCTGTATTGAAGGTCTTGGAGTGAATTGGGGAACTATGGCGAATCATAAATTACCCCCTAAGACCGTTGTGCAGATGTTTAAGGATAATGGGATTCAGAAAGTGAAGCTGTTTGATGCAGACCAATCCTCCATGGGCGCTCTGGCTGGCACTGGTATTGAGGTCATGGTTGCCATTCCTAATGATCAACTTGTCGCCATGGGAGACTACAATCGAGCTAAGCAATGGGTGCAGAGAAATGTCTCCCGTTACACCTTTGATGGAGGAGTTACCATCAAGTAATTCATTTCAAACCACACAAAAATTTTTAAGCTAAGCCTGTGTTTTGTTCTTACTATGATGTTCTTGTACAGATATGTTGCTGTTGGCAATGAGCCATTTCTATCCTCTTATAACGGATCATTCTTGAATGTTACCTTTCCAGCCCTTCAAAACATTCAAAATGCACTTAATGAAGCTGGTTATGGGGACACCATCAAGGCAACGGTACCCTTAAATGCTGATGTTTACAACTCCCCGGTGAACACCCCGTATCCGTCTGCTGGTCGGTTTCGGAGCGATATCAATGGACTCATGACTCAAATTGTCCAGTTTCTAAGCAAGAACAATGCACCATTTACTGTCAACATATACCCTTTTCTTAGTCTTTATGGCAATGACAACTTCCCATTCGACTATGCTTTCTTTGATGGTGCAAGCAATCCTGTTGTTGACATGAACACCGGGATTCAATATACAAATGTTTTCGACGCGAACTTCGACACGTTGGTGTCGTCTCTGAAAGCTGTAGGACTTGGGGACATGCCAATTCTAGTTGGCGAGGTTGGATGGCCTACTGATGGTGATAAGAATGCCAATGCTGGCAATGCTTATAGATTCTACAATGGCCTTCTACCAAGGCTTGCAGCCAACAAAGGCACCCCATTGAGGCCTGGATTCATTGAAGTGTACTTGTTTAGCTTTCTTGATGAGAATGGGAAGAGCATTGCTCCAGGGAACTTCGAGCGGCATTGGGGCATTTTCGGGTACGACGGCCAGCCGAAGTTCGGCATGGATCTTTCAGGTCAGGGCCAGAACAAGCTGCTTGTGGGAGCACAAGACGTGCAGTATCTTCCTCAAAAGTGGTGTATGTTCAATCCAAATGCTAAGGATTTAAGCAAACTAGCTGATAACATAGACTATGCGTGCACTTTTTCTGATTGCACTTCGCTTGGGTATGGATCTTCTTGCAATAATTTGGATGCTAATGGGAATGCGTCGTATGCGTTTAATATGTACTTTCAGGTGCAGAATCAAGATGAATTGGCGTGTAATTTTCAAGGGTTAGCTACAATAACCTCGCAGAATCTTTCACAAGGTACTTGTAAGTTCATCATCCAGCTAGCTTCGTCTTCCCCCTCCTTGGATGCTTCATTTGTTAGCTTAGTATCTATGGCGTTGTTATTCATAGCAATGTGGCTATGATTTGGTTGTGTAGATACTTTTTGATGATGATGATGATGATGATGTTCCATATTATTGCTCTTATACTTGGCTACATTTTTTCTAATGTTCATTAGTTTATGATTGATTCTATTCAATCATCTCTTCTGCATTTTGTGTTGGTGGAATAGTGCATATGAAAAGAGGCATTAATGGATAATGGATGTTATAATGTAATTGTTAGAACGTAATTGTAACCGTCCAAGTAGTGCTGAGGGCATTATATTAGAAATTGGACACAAGGATGGTTCATATGTGCACCAAGA

mRNA sequence

GGAAGACGGAAAACCCTTCAGTTTTTTTTCTTTTTTTCCCCATCTTTTTCTATAATCAATCTGTTCAGATCACTTTCATTTGCTAATTCCATCTGGGTATCATTGCTTTTTGTTATTTCATTCTTTTTCCCCGTTTTGTTGGCTGTGAGCGAGGTGGGAAAATGGGAAGATTATGGATTCTGAAATGGGTTTTGTTGGTTGGGGTTTTGGGTTGCTGTATTGAAGGTCTTGGAGTGAATTGGGGAACTATGGCGAATCATAAATTACCCCCTAAGACCGTTGTGCAGATGTTTAAGGATAATGGGATTCAGAAAGTGAAGCTGTTTGATGCAGACCAATCCTCCATGGGCGCTCTGGCTGGCACTGGTATTGAGGTCATGGTTGCCATTCCTAATGATCAACTTGTCGCCATGGGAGACTACAATCGAGCTAAGCAATGGGTGCAGAGAAATGTCTCCCGTTACACCTTTGATGGAGGAGTTACCATCAAATATGTTGCTGTTGGCAATGAGCCATTTCTATCCTCTTATAACGGATCATTCTTGAATGTTACCTTTCCAGCCCTTCAAAACATTCAAAATGCACTTAATGAAGCTGGTTATGGGGACACCATCAAGGCAACGGTACCCTTAAATGCTGATGTTTACAACTCCCCGGTGAACACCCCGTATCCGTCTGCTGGTCGGTTTCGGAGCGATATCAATGGACTCATGACTCAAATTGTCCAGTTTCTAAGCAAGAACAATGCACCATTTACTGTCAACATATACCCTTTTCTTAGTCTTTATGGCAATGACAACTTCCCATTCGACTATGCTTTCTTTGATGGTGCAAGCAATCCTGTTGTTGACATGAACACCGGGATTCAATATACAAATGTTTTCGACGCGAACTTCGACACGTTGGTGTCGTCTCTGAAAGCTGTAGGACTTGGGGACATGCCAATTCTAGTTGGCGAGGTTGGATGGCCTACTGATGGTGATAAGAATGCCAATGCTGGCAATGCTTATAGATTCTACAATGGCCTTCTACCAAGGCTTGCAGCCAACAAAGGCACCCCATTGAGGCCTGGATTCATTGAAGTGTACTTGTTTAGCTTTCTTGATGAGAATGGGAAGAGCATTGCTCCAGGGAACTTCGAGCGGCATTGGGGCATTTTCGGGTACGACGGCCAGCCGAAGTTCGGCATGGATCTTTCAGGTCAGGGCCAGAACAAGCTGCTTGTGGGAGCACAAGACGTGCAGTATCTTCCTCAAAAGTGGTGTATGTTCAATCCAAATGCTAAGGATTTAAGCAAACTAGCTGATAACATAGACTATGCGTGCACTTTTTCTGATTGCACTTCGCTTGGGTATGGATCTTCTTGCAATAATTTGGATGCTAATGGGAATGCGTCGTATGCGTTTAATATGTACTTTCAGGTGCAGAATCAAGATGAATTGGCGTGTAATTTTCAAGGGTTAGCTACAATAACCTCGCAGAATCTTTCACAAGGTACTTGTAAGTTCATCATCCAGCTAGCTTCGTCTTCCCCCTCCTTGGATGCTTCATTTGTTAGCTTAGTATCTATGGCGTTGTTATTCATAGCAATGTGGCTATGATTTGGTTGTGTAGATACTTTTTGATGATGATGATGATGATGATGTTCCATATTATTGCTCTTATACTTGGCTACATTTTTTCTAATGTTCATTAGTTTATGATTGATTCTATTCAATCATCTCTTCTGCATTTTGTGTTGGTGGAATAGTGCATATGAAAAGAGGCATTAATGGATAATGGATGTTATAATGTAATTGTTAGAACGTAATTGTAACCGTCCAAGTAGTGCTGAGGGCATTATATTAGAAATTGGACACAAGGATGGTTCATATGTGCACCAAGA

Coding sequence (CDS)

ATGGGAAGATTATGGATTCTGAAATGGGTTTTGTTGGTTGGGGTTTTGGGTTGCTGTATTGAAGGTCTTGGAGTGAATTGGGGAACTATGGCGAATCATAAATTACCCCCTAAGACCGTTGTGCAGATGTTTAAGGATAATGGGATTCAGAAAGTGAAGCTGTTTGATGCAGACCAATCCTCCATGGGCGCTCTGGCTGGCACTGGTATTGAGGTCATGGTTGCCATTCCTAATGATCAACTTGTCGCCATGGGAGACTACAATCGAGCTAAGCAATGGGTGCAGAGAAATGTCTCCCGTTACACCTTTGATGGAGGAGTTACCATCAAATATGTTGCTGTTGGCAATGAGCCATTTCTATCCTCTTATAACGGATCATTCTTGAATGTTACCTTTCCAGCCCTTCAAAACATTCAAAATGCACTTAATGAAGCTGGTTATGGGGACACCATCAAGGCAACGGTACCCTTAAATGCTGATGTTTACAACTCCCCGGTGAACACCCCGTATCCGTCTGCTGGTCGGTTTCGGAGCGATATCAATGGACTCATGACTCAAATTGTCCAGTTTCTAAGCAAGAACAATGCACCATTTACTGTCAACATATACCCTTTTCTTAGTCTTTATGGCAATGACAACTTCCCATTCGACTATGCTTTCTTTGATGGTGCAAGCAATCCTGTTGTTGACATGAACACCGGGATTCAATATACAAATGTTTTCGACGCGAACTTCGACACGTTGGTGTCGTCTCTGAAAGCTGTAGGACTTGGGGACATGCCAATTCTAGTTGGCGAGGTTGGATGGCCTACTGATGGTGATAAGAATGCCAATGCTGGCAATGCTTATAGATTCTACAATGGCCTTCTACCAAGGCTTGCAGCCAACAAAGGCACCCCATTGAGGCCTGGATTCATTGAAGTGTACTTGTTTAGCTTTCTTGATGAGAATGGGAAGAGCATTGCTCCAGGGAACTTCGAGCGGCATTGGGGCATTTTCGGGTACGACGGCCAGCCGAAGTTCGGCATGGATCTTTCAGGTCAGGGCCAGAACAAGCTGCTTGTGGGAGCACAAGACGTGCAGTATCTTCCTCAAAAGTGGTGTATGTTCAATCCAAATGCTAAGGATTTAAGCAAACTAGCTGATAACATAGACTATGCGTGCACTTTTTCTGATTGCACTTCGCTTGGGTATGGATCTTCTTGCAATAATTTGGATGCTAATGGGAATGCGTCGTATGCGTTTAATATGTACTTTCAGGTGCAGAATCAAGATGAATTGGCGTGTAATTTTCAAGGGTTAGCTACAATAACCTCGCAGAATCTTTCACAAGGTACTTGTAAGTTCATCATCCAGCTAGCTTCGTCTTCCCCCTCCTTGGATGCTTCATTTGTTAGCTTAGTATCTATGGCGTTGTTATTCATAGCAATGTGGCTATGA

Protein sequence

MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL

Homology

[Close]

BLAST of Carg27692 vs. NCBI nr
Match: KAG7019320.1 (Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 981.5 bits (2536), Expect = 2.6e-282
Identity = 479/479 (100.00%), Postives = 479/479 (100.00%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL
Sbjct: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 479

[Close]

BLAST of Carg27692 vs. NCBI nr
Match: KAG6581686.1 (Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 979.5 bits (2531), Expect = 1.0e-281
Identity = 478/479 (99.79%), Postives = 478/479 (99.79%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLF AMWL
Sbjct: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFTAMWL 479

[Close]

BLAST of Carg27692 vs. NCBI nr
Match: XP_022932796.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata])

HSP 1 Score: 974.9 bits (2519), Expect = 2.5e-280
Identity = 475/479 (99.16%), Postives = 477/479 (99.58%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLAT+TSQNLSQGTCKFIIQLASSSPSLD SFVSLVSMALLF AMW+
Sbjct: 421 VQNQDELACNFQGLATMTSQNLSQGTCKFIIQLASSSPSLDDSFVSLVSMALLFTAMWV 479

[Close]

BLAST of Carg27692 vs. NCBI nr
Match: XP_023526667.1 (glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 970.7 bits (2508), Expect = 4.6e-279
Identity = 473/479 (98.75%), Postives = 476/479 (99.37%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MGRLWILKWVLLVGVLGCC+EGLGVNWGTMANHKLPPKT+VQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAGYG+TIKATVPLNADVYNSPVNTPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGETIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPS DASFVSLVSMALLF AM L
Sbjct: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSFDASFVSLVSMALLFTAMLL 479

[Close]

BLAST of Carg27692 vs. NCBI nr
Match: XP_022974227.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima])

HSP 1 Score: 967.2 bits (2499), Expect = 5.1e-278
Identity = 471/479 (98.33%), Postives = 475/479 (99.16%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MGRLWIL WVLLVGVLGCCIEGLGVNWGTMANHKLPPKT+VQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILNWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQN+QNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNVQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD+NTGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDVNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLATI+SQNLSQGTCKFIIQLASSSPS DASFVSLVSMALLF AM L
Sbjct: 421 VQNQDELACNFQGLATISSQNLSQGTCKFIIQLASSSPSFDASFVSLVSMALLFTAMLL 479

[Close]

BLAST of Carg27692 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 725.3 bits (1871), Expect = 4.4e-208
Identity = 352/471 (74.73%), Postives = 402/471 (85.35%), Query Frame = 0


Query: 6   ILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGAL 65
           ++ +V+++G LG  + GLGVNWGTMA HKLPPKTVVQM KDN I KVKLFDAD+++MGAL
Sbjct: 8   VVGFVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGAL 67

Query: 66  AGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYNG 125
           AG+G+EVMVAIPNDQL  M  Y+RAK WV++NV+RY FDGGV I +VAVGNEPFL SYNG
Sbjct: 68  AGSGLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNG 127

Query: 126 SFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLMT 185
           SF+N+TFPAL NIQNALNEAG G+++KATVPLNADVY+SP + P PSAGRFR DI G MT
Sbjct: 128 SFINLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMT 187

Query: 186 QIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFDANF 245
           QIV FL KNNAP T+NIYPFLSLYGND+FP +YAFFDGA  P+ D   GI YTNVFDANF
Sbjct: 188 QIVDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANF 247

Query: 246 DTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGF 305
           DTLVSSLKAVG GDMPI+VGEVGWPT+GDK+ANAG+AYRFYNGLLPRL  NKGTPLRP +
Sbjct: 248 DTLVSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTY 307

Query: 306 IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPQ 365
           IEVYLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQ+K L+GAQ+V YLP 
Sbjct: 308 IEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPN 367

Query: 366 KWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQD 425
           KWC FNP AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQD
Sbjct: 368 KWCTFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQD 427

Query: 426 ELACNFQGLATITSQNLSQGTCKFIIQL-ASSSPSLDASFVSLVSMALLFI 476
           E AC FQGLATIT+QN+SQG C F IQ+ ASS+ S   S  SLV + + F+
Sbjct: 428 ESACYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFL 475

[Close]

BLAST of Carg27692 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.1e-137
Identity = 249/460 (54.13%), Postives = 322/460 (70.00%), Query Frame = 0


Query: 20  IEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGALAGTGIEVMVAIPND 79
           +EG+GVNWG+ A H LPP TVV++ ++NGIQKVKLF+AD + + AL+ TGI+VMV IPND
Sbjct: 24  VEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKALSRTGIQVMVGIPND 83

Query: 80  QLVAM-GDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYNGSFLNVTFPALQNI 139
            L  + G    A++WV +NVS +    GV I+YVAVGNEPFL ++NG+F  +T PALQNI
Sbjct: 84  LLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGTFEGITLPALQNI 143

Query: 140 QNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLMTQIVQFLSKNNAPF 199
           Q+A+ +AG    +K TVPLNADVY S  N   PS G FR +I  LM  IV+FLS N APF
Sbjct: 144 QSAIIKAGLATQVKVTVPLNADVYQSASN--LPSDGDFRPEIRDLMLNIVKFLSDNGAPF 203

Query: 200 TVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFDANFDTLVSSLKAVGLG 259
           T+NIYPF+SLY + NFP ++AFFDG   P+ D   G  Y NV DAN+DTLV SL+  G G
Sbjct: 204 TINIYPFISLYNDPNFPVEFAFFDGTGTPIND--NGRIYDNVLDANYDTLVWSLQKNGFG 263

Query: 260 DMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEVYLFSFLDENG 319
           ++ I+VGEVGWPTDGDKNAN   A R+  G + R  ANKGTP+RPG ++ YLF  +DE+ 
Sbjct: 264 NLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAMDAYLFGLIDEDA 323

Query: 320 KSIAPGNFERHWGIFGYDGQPKFGMDL-SGQGQNKLLVGAQDVQYLPQKWCMFNPNAK-D 379
           KSI PGNFERHWGIF  DGQPK+ + L SG G    L+ A+DV YL +KWC+  PNA   
Sbjct: 324 KSIQPGNFERHWGIFYIDGQPKYQLSLGSGNG----LIPAKDVHYLAKKWCILAPNANLQ 383

Query: 380 LSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELACNFQGLAT 439
             +L  ++ YAC  +DCTSLGYGSSC NL+   N SYAFN Y+QV NQ + AC F GL+ 
Sbjct: 384 DPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSACKFPGLSI 443

Query: 440 ITSQNLSQGTCKFIIQLASSSPSLDASFVSLV-SMALLFI 476
           +++++ S G+CKF I + S   S  ++ + +  S A+L +
Sbjct: 444 VSTRDPSVGSCKFKIMIKSEDASEASAMMPITRSTAVLLL 475

[Close]

BLAST of Carg27692 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 476.5 bits (1225), Expect = 3.6e-133
Identity = 233/446 (52.24%), Postives = 312/446 (69.96%), Query Frame = 0


Query: 10  VLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGALAGTG 69
           +L V +L      +G NWGT A+H LPP  VV+M ++NGIQKVKLFDA+  ++ AL  +G
Sbjct: 9   LLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRALGKSG 68

Query: 70  IEVMVAIPNDQLVAM-GDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYNGSFL 129
           IEVMV IPN+ L  +      A++WV +NVS +     V I+YVAVGNEPFLS+YNGS+L
Sbjct: 69  IEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTYNGSYL 128

Query: 130 NVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLMTQIV 189
           + TFPAL+NIQ A+ +AG  + +K T PLNADVY+S  +T +PS G FR++I  LM  IV
Sbjct: 129 STTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDS--STTFPSGGDFRANIRDLMITIV 188

Query: 190 QFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFDANFDTL 249
           +FLS+N  PFTVNIYP++SLY N +FP DYAFFDG + P+ D   G  Y N+FDAN+DTL
Sbjct: 189 KFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLND--GGTFYYNMFDANYDTL 248

Query: 250 VSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEV 309
           V +L+  G G+MPI++GE+GWPTDGD NAN   A +F  G +  ++  KGTP RPG I+ 
Sbjct: 249 VHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPGPIDA 308

Query: 310 YLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPQKWC 369
           YLFS +DE+ KS+ PG FERHWGIF +DG PK+ ++L G      L+ A+ V+YL +KWC
Sbjct: 309 YLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLERKWC 368

Query: 370 MFNPNAK-DLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEL 429
           +  PN + D  ++A  + YAC+  DCTSLG G+SC NLD   N SYAFN Y+Q+Q+Q + 
Sbjct: 369 VMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLDT 428

Query: 430 ACNFQGLATITSQNLSQGTCKFIIQL 454
           AC F  ++ +T  + S GTC+F I +
Sbjct: 429 ACKFPNISEVTKTDPSTGTCRFPIMI 449

[Close]

BLAST of Carg27692 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 3.0e-95
Identity = 199/479 (41.54%), Postives = 283/479 (59.08%), Query Frame = 0


Query: 3   RLWILKWVLLVG--VLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 62
           RL++L   +  G  + G  +  +G+NWGT A+H LPP  VV++ K NGI KVKLFDAD  
Sbjct: 4   RLFLLLLAVTAGLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPK 63

Query: 63  SMGALAGTGIEVMVAIPNDQLVAM-GDYNRAKQWVQRNVSRYTFDGG--VTIKYVAVGNE 122
            + AL+G+ I V + I N  L ++      A+ WV  NV+RY F+GG  V I+YVAVG E
Sbjct: 64  VLRALSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAVGEE 123

Query: 123 PFLSSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFR 182
           PFL SY   +      A  NIQNAL +A   + +K  VP + D + S   +  PS+G FR
Sbjct: 124 PFLQSYGNQYKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLS--ESGRPSSGHFR 183

Query: 183 SDINGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQY 242
           +D+N  M +++ FL+K+++PF V I PFLS + N N   D++ F   +    D      Y
Sbjct: 184 ADLNKTMIELLSFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRK--TY 243

Query: 243 TNVFDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANK 302
            N FD ++DTLVS+L  +G  ++ I+V ++GWPTDG +NA +  A  F+ GL+  L    
Sbjct: 244 RNSFDLSYDTLVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKT 303

Query: 303 GTPLRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGA 362
            +  RP  +E Y+ S LDE+ ++++ GNFERHWG+F +DGQ K+    + + Q    V A
Sbjct: 304 ASLPRPP-VETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFNHKNQ----VNA 363

Query: 363 QDVQYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNM 422
           Q+VQYLP KWC+ N N KDLS  +     AC  +DCTS+  G SC+ +   GN SYAFN 
Sbjct: 364 QNVQYLPPKWCVVN-NNKDLSNASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNS 423

Query: 423 YFQVQNQDELACNFQGLATITSQNLSQGTCKFIIQL-ASSSPSLDASFVSLVSMALLFI 476
            +Q  +    +CNF GL  IT+ + S+  C+F IQL  S S S   +F     + LLF+
Sbjct: 424 LYQQNDHSAESCNFGGLGLITTVDPSEDNCRFSIQLDTSHSSSQTPNFFQSWPLLLLFL 471

[Close]

BLAST of Carg27692 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 220.3 bits (560), Expect = 4.6e-56
Identity = 150/438 (34.25%), Postives = 230/438 (52.51%), Query Frame = 0


Query: 23  LGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGALAGTGIEVMVAIPNDQLV 82
           +GVN+G +A++  PP   V++ +   IQKV+L+ AD + + ALAGTG+ +++   N  + 
Sbjct: 26  IGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVP 85

Query: 83  AM-GDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQNA 142
           ++  D N A QW+  NV    F     I  + VGNE  L S + + +N   PA+QN+Q A
Sbjct: 86  SLASDPNAATQWINSNV--LPFYPASKIMLITVGNE-ILMSNDPNLVNQLLPAMQNVQKA 145

Query: 143 LNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLMTQIVQFLSKNNAPFTVN 202
           L     G  IK +   +  V  S   +  PS+G F +     +  I+QFLS   +PF +N
Sbjct: 146 LEAVSLGGKIKVSTVNSMTVLGS---SDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAIN 205

Query: 203 IYPFLSLYGNDNFPFDYAFFDGASNP-VVDMNTGIQYTNVFDANFDTLVSSLKAVGLGDM 262
            YPF + Y +D  P   AF     N   VD  TGI+YTN+FDA  D + S+LK++G   +
Sbjct: 206 PYPFFA-YQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKV 265

Query: 263 PILVGEVGWPTDGDKN---ANAGNAYRFYNGLLPRLAANKGTPLRPGF-IEVYLFSFLDE 322
            I+V E GW + GD N   A+  NA  +   L+  L +  GTPL PG  ++ Y+F+  DE
Sbjct: 266 EIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDE 325

Query: 323 NGKSIAPG-NFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPQKWCMFNPNAK 382
           N   + PG + ER +G+F  D    + + L+    +         +     WC+    A 
Sbjct: 326 N---LKPGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSG---KVTSSGWCVPKKGAT 385

Query: 383 DLSKLADNIDYACTFS-DCTSLGYGSSC---NNLDANGNASYAFNMYFQVQNQDELACNF 442
           +  +L  ++D+AC    DC ++  G +C   NN+ +  +A+YA NMYFQ   +    C+F
Sbjct: 386 N-EELQASLDWACGHGIDCGAIQPGGACFEPNNVVS--HAAYAMNMYFQKSPKQPTDCDF 445

Query: 443 QGLATITSQNLSQGTCKF 450
              AT+TSQN S   C +
Sbjct: 446 SKTATVTSQNPSYNNCVY 447

[Close]

BLAST of Carg27692 vs. ExPASy TrEMBL
Match: A0A6J1EXE0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111439258 PE=3 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.2e-280
Identity = 475/479 (99.16%), Postives = 477/479 (99.58%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLAT+TSQNLSQGTCKFIIQLASSSPSLD SFVSLVSMALLF AMW+
Sbjct: 421 VQNQDELACNFQGLATMTSQNLSQGTCKFIIQLASSSPSLDDSFVSLVSMALLFTAMWV 479

[Close]

BLAST of Carg27692 vs. ExPASy TrEMBL
Match: A0A6J1I9Q3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472848 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.5e-278
Identity = 471/479 (98.33%), Postives = 475/479 (99.16%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MGRLWIL WVLLVGVLGCCIEGLGVNWGTMANHKLPPKT+VQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILNWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQN+QNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNVQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD+NTGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDVNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLATI+SQNLSQGTCKFIIQLASSSPS DASFVSLVSMALLF AM L
Sbjct: 421 VQNQDELACNFQGLATISSQNLSQGTCKFIIQLASSSPSFDASFVSLVSMALLFTAMLL 479

[Close]

BLAST of Carg27692 vs. ExPASy TrEMBL
Match: A0A0A0LFN2 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G843830 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.7e-263
Identity = 448/479 (93.53%), Postives = 460/479 (96.03%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MG L  LKWVLLVGVLG C+EGLGVNWGTMANH+LPPKTVVQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGSLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQL AMGDYNRAKQWVQRNV+RYTFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPAL NIQNALNEAG+GD+IKATVPLNADVYNSPVN+PYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALLNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           N LMT IVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD+ TGIQYTNV
Sbjct: 181 NQLMTDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKN N GNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNGNVGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLAT+TSQNLSQGTC FIIQLASS PS  ASFVSL SM LLFIA+ L
Sbjct: 421 VQNQDELACNFQGLATVTSQNLSQGTCNFIIQLASSPPSHAASFVSLASMILLFIALLL 479

[Close]

BLAST of Carg27692 vs. ExPASy TrEMBL
Match: A0A5D3E281 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00130 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.0e-263
Identity = 448/479 (93.53%), Postives = 460/479 (96.03%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MGRL  LKWVLLVGVLG C+EGLGVNWGTMANH+LPPKTVVQM KDNGIQKVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQL AMGDYNRAKQWVQRNV+RY FDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG+GD+IKATVPLNADVYNSPVN+PYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           N LM  IVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD+ TGIQYTNV
Sbjct: 181 NQLMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           +YLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 EYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLATITS+NLSQGTC FIIQLASS PS  ASFVSL SM LLFIA+ L
Sbjct: 421 VQNQDELACNFQGLATITSRNLSQGTCNFIIQLASSPPSHAASFVSLASMVLLFIALLL 479

[Close]

BLAST of Carg27692 vs. ExPASy TrEMBL
Match: A0A1S3CNJ0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103502975 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.0e-263
Identity = 448/479 (93.53%), Postives = 460/479 (96.03%), Query Frame = 0


Query: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60
           MGRL  LKWVLLVGVLG C+EGLGVNWGTMANH+LPPKTVVQM KDNGIQKVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQL AMGDYNRAKQWVQRNV+RY FDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG+GD+IKATVPLNADVYNSPVN+PYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240
           N LM  IVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD+ TGIQYTNV
Sbjct: 181 NQLMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           +YLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 EYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 480
           VQNQDELACNFQGLATITS+NLSQGTC FIIQLASS PS  ASFVSL SM LLFIA+ L
Sbjct: 421 VQNQDELACNFQGLATITSRNLSQGTCNFIIQLASSPPSHAASFVSLASMVLLFIALLL 479

[Close]

BLAST of Carg27692 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 725.3 bits (1871), Expect = 3.2e-209
Identity = 352/471 (74.73%), Postives = 402/471 (85.35%), Query Frame = 0


Query: 6   ILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGAL 65
           ++ +V+++G LG  + GLGVNWGTMA HKLPPKTVVQM KDN I KVKLFDAD+++MGAL
Sbjct: 8   VVGFVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGAL 67

Query: 66  AGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYNG 125
           AG+G+EVMVAIPNDQL  M  Y+RAK WV++NV+RY FDGGV I +VAVGNEPFL SYNG
Sbjct: 68  AGSGLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNG 127

Query: 126 SFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLMT 185
           SF+N+TFPAL NIQNALNEAG G+++KATVPLNADVY+SP + P PSAGRFR DI G MT
Sbjct: 128 SFINLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMT 187

Query: 186 QIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFDANF 245
           QIV FL KNNAP T+NIYPFLSLYGND+FP +YAFFDGA  P+ D   GI YTNVFDANF
Sbjct: 188 QIVDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANF 247

Query: 246 DTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGF 305
           DTLVSSLKAVG GDMPI+VGEVGWPT+GDK+ANAG+AYRFYNGLLPRL  NKGTPLRP +
Sbjct: 248 DTLVSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTY 307

Query: 306 IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPQ 365
           IEVYLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQ+K L+GAQ+V YLP 
Sbjct: 308 IEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPN 367

Query: 366 KWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQD 425
           KWC FNP AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQD
Sbjct: 368 KWCTFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQD 427

Query: 426 ELACNFQGLATITSQNLSQGTCKFIIQL-ASSSPSLDASFVSLVSMALLFI 476
           E AC FQGLATIT+QN+SQG C F IQ+ ASS+ S   S  SLV + + F+
Sbjct: 428 ESACYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFL 475

[Close]

BLAST of Carg27692 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 725.3 bits (1871), Expect = 3.2e-209
Identity = 352/471 (74.73%), Postives = 402/471 (85.35%), Query Frame = 0


Query: 6   ILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGAL 65
           ++ +V+++G LG  + GLGVNWGTMA HKLPPKTVVQM KDN I KVKLFDAD+++MGAL
Sbjct: 8   VVGFVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGAL 67

Query: 66  AGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYNG 125
           AG+G+EVMVAIPNDQL  M  Y+RAK WV++NV+RY FDGGV I +VAVGNEPFL SYNG
Sbjct: 68  AGSGLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNG 127

Query: 126 SFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLMT 185
           SF+N+TFPAL NIQNALNEAG G+++KATVPLNADVY+SP + P PSAGRFR DI G MT
Sbjct: 128 SFINLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMT 187

Query: 186 QIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFDANF 245
           QIV FL KNNAP T+NIYPFLSLYGND+FP +YAFFDGA  P+ D   GI YTNVFDANF
Sbjct: 188 QIVDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANF 247

Query: 246 DTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGF 305
           DTLVSSLKAVG GDMPI+VGEVGWPT+GDK+ANAG+AYRFYNGLLPRL  NKGTPLRP +
Sbjct: 248 DTLVSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTY 307

Query: 306 IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPQ 365
           IEVYLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQ+K L+GAQ+V YLP 
Sbjct: 308 IEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPN 367

Query: 366 KWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQD 425
           KWC FNP AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQD
Sbjct: 368 KWCTFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQD 427

Query: 426 ELACNFQGLATITSQNLSQGTCKFIIQL-ASSSPSLDASFVSLVSMALLFI 476
           E AC FQGLATIT+QN+SQG C F IQ+ ASS+ S   S  SLV + + F+
Sbjct: 428 ESACYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFL 475

[Close]

BLAST of Carg27692 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 706.8 bits (1823), Expect = 1.2e-203
Identity = 346/474 (73.00%), Postives = 400/474 (84.39%), Query Frame = 0


Query: 6   ILKWVLLVGVLG-CCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGA 65
           +L +V+  G++G   ++GLGVNWGTMA HKLPPK VVQM KDN I KVKLFDAD+++M A
Sbjct: 3   LLAFVVGFGIMGIVMVDGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSA 62

Query: 66  LAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYN 125
           L+G+G+EVMVAIPNDQL  MG Y+RAK WV +NV+RY F+GGV I +VAVGNEPFL SYN
Sbjct: 63  LSGSGLEVMVAIPNDQLKVMGSYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYN 122

Query: 126 GSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLM 185
           GSF+N+TFPALQNIQNALNEAG G ++KATVPLNADVY+SP + P PSAGRFR DI G M
Sbjct: 123 GSFINLTFPALQNIQNALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQM 182

Query: 186 TQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFDAN 245
           TQIV FL  N+AP T+NIYPFLSLYGND+FP +YAFFDGA  PV D   GI YTNVFDAN
Sbjct: 183 TQIVDFLGNNSAPITINIYPFLSLYGNDDFPLNYAFFDGA-KPVDD--NGIAYTNVFDAN 242

Query: 246 FDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPG 305
           FDTLVS+LKAVG GDMPI+VGEVGWPT+GDK+AN+G+AYRFYNGLLPRL  N+GTPLRP 
Sbjct: 243 FDTLVSALKAVGHGDMPIIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPT 302

Query: 306 FIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLP 365
           +IEVYLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQNKLL+GA++V Y P
Sbjct: 303 YIEVYLFGLLDEDAKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQP 362

Query: 366 QKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQ 425
           +KWCMFN  AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNMYFQV+NQ
Sbjct: 363 KKWCMFNTEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQ 422

Query: 426 DELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMW 479
           DE AC FQGLATIT++N+SQG C F IQ+ +S+ S   SF S  S+ LL   +W
Sbjct: 423 DEDACIFQGLATITTKNISQGQCNFPIQIVASTAS---SFSS--SLVLLIAGVW 468

[Close]

BLAST of Carg27692 vs. TAIR 10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 661.4 bits (1705), Expect = 5.6e-190
Identity = 322/456 (70.61%), Postives = 380/456 (83.33%), Query Frame = 0


Query: 20  IEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGALAGTGIEVMVAIPND 79
           + GLGVNWGTMA+H+LPPKTVV+M KDN IQKVKLFDAD ++MGALAG+G+EVMVAIPND
Sbjct: 30  VSGLGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNTMGALAGSGVEVMVAIPND 89

Query: 80  QLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQ 139
            L+AMG+Y RAK WVQRNVSR+ F+ GV IKYVAVGNEPFL++YNGSF+N+T+PAL NIQ
Sbjct: 90  LLLAMGNYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGSFINLTYPALFNIQ 149

Query: 140 NALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLMTQIVQFLSKNNAPFT 199
            ALNEAG GD  KATVPLNADVYNSP +   PSAGRFRSDI   MTQIV FL++N APFT
Sbjct: 150 TALNEAGVGDFTKATVPLNADVYNSPPDNQVPSAGRFRSDIIQEMTQIVNFLAQNKAPFT 209

Query: 200 VNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFDANFDTLVSSLKAVGLGD 259
           VNIYPFLSLY + +FPF+YAFFDG  N V D   G+ YTNVFDA+FDTL++SL A+  G+
Sbjct: 210 VNIYPFLSLYLSSDFPFEYAFFDG-QNTVND--NGVIYTNVFDASFDTLLASLNALNHGN 269

Query: 260 MPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEVYLFSFLDENGK 319
           M ++VGEVGWPTDGDKNAN  NA RFY+GLLPRLA N GTP+R G+IEVYLF F+DE+ K
Sbjct: 270 MEVIVGEVGWPTDGDKNANVPNAERFYSGLLPRLANNVGTPMRKGYIEVYLFGFIDEDAK 329

Query: 320 SIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPQKWCMFNPNAK-DLS 379
           S+APGNFERHWGIF +DGQPKF +D  GQGQ K L GAQ+VQY   +WCMFNPN + ++S
Sbjct: 330 SVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWCMFNPNGRGNMS 389

Query: 380 KLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELACNFQGLATIT 439
           +L DNI+YAC+ SDCT+LGYGSSC NLDANGNASYAFNMYFQVQNQ+  AC+F+GLATIT
Sbjct: 390 RLGDNINYACSHSDCTALGYGSSCGNLDANGNASYAFNMYFQVQNQEAQACDFEGLATIT 449

Query: 440 SQNLSQGTCKFIIQLAS-SSPSLDASFVSLVSMALL 474
           +QN+SQG C F IQ+   SS   D S+ S+V + L+
Sbjct: 450 TQNISQGQCNFPIQIGEPSSGHYDYSYGSMVRLCLV 482

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BLAST of Carg27692 vs. TAIR 10
Match: AT5G18220.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 646.4 bits (1666), Expect = 1.9e-185
Identity = 325/477 (68.13%), Postives = 381/477 (79.87%), Query Frame = 0


Query: 10  VLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSMGALAGTG 69
           + +V +L   +  LGVNWGTM+ H+LPPKTVVQM KDN ++KVKLFDAD ++M ALAG+G
Sbjct: 17  ITIVSLLSGDVSALGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADTNTMVALAGSG 76

Query: 70  IEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSSYNGSFLN 129
           IEVMVAIPNDQL AMG YNRAK WV+RN++R+  D  V IKYVAVGNEPFL++YNGSF+N
Sbjct: 77  IEVMVAIPNDQLKAMGSYNRAKDWVRRNITRFNDD--VKIKYVAVGNEPFLTAYNGSFIN 136

Query: 130 VTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDINGLMTQIVQ 189
           +T+PAL NIQ ALNEAG GD IKATVPLNADVYNSP+  P PSAG FR DI   M  IV 
Sbjct: 137 LTYPALFNIQKALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIFEEMKLIVN 196

Query: 190 FLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFDANFDTLV 249
           FL+ N APFTVNIYPFLSLY + +FPFDYAFF+G  N V D   G+ YTNVFDANFDTL+
Sbjct: 197 FLAHNKAPFTVNIYPFLSLYLSSDFPFDYAFFNG-QNTVSD--NGVIYTNVFDANFDTLL 256

Query: 250 SSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEVY 309
           +SLKA+G GDM ++VGEVGWPTDGDKNAN  NA RFY GLLP+LAAN+GTP+RPG+IEVY
Sbjct: 257 ASLKALGHGDMTVIVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMRPGYIEVY 316

Query: 310 LFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPQKWCM 369
           LF F+DE+ KSIAPGNFERHWGIF YDGQPKF  DLSG GQ K+L GAQ+VQYL  +WCM
Sbjct: 317 LFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLRNQWCM 376

Query: 370 FNPNA----KDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQD 429
           FNPNA     + ++L DN++YACTFSDCT+LGYGSSC NLD  GNASYAFNMYFQVQNQ 
Sbjct: 377 FNPNALTFSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNMYFQVQNQK 436

Query: 430 ELACNFQGLATITSQNLSQGTCKFIIQL---ASSSPSLDASFV--SLVSMALLFIAM 478
             AC+F+GLA IT++N+S+  C F IQ+    S     +  F+  SLVSM LLF A+
Sbjct: 437 AEACDFEGLAIITTRNISREQCNFPIQIGDPTSGHSDHNHRFISFSLVSMLLLFTAL 488

The following BLAST results are available for this feature:

BLAST of Carg27692 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019320.1	2.6e-282	100.00	Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6581686.1	1.0e-281	99.79	Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022932796.1	2.5e-280	99.16	glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata]	[more]
XP_023526667.1	4.6e-279	98.75	glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022974227.1	5.1e-278	98.33	glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima]	[more]

BLAST of Carg27692 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NKW9	4.4e-208	74.73	Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ...	[more]
Q9M088	3.1e-137	54.13	Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ...	[more]
Q93Z08	3.6e-133	52.24	Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ...	[more]
Q9FGH4	3.0e-95	41.54	Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ...	[more]
Q9M069	4.6e-56	34.25	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]

BLAST of Carg27692 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EXE0	1.2e-280	99.16	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111439258 P...	[more]
A0A6J1I9Q3	2.5e-278	98.33	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472848 PE=...	[more]
A0A0A0LFN2	1.7e-263	93.53	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G843830 PE=3...	[more]
A0A5D3E281	5.0e-263	93.53	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CNJ0	5.0e-263	93.53	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103502975 PE=3 SV...	[more]

BLAST of Carg27692 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64760.1	3.2e-209	74.73	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.2	3.2e-209	74.73	O-Glycosyl hydrolases family 17 protein	[more]
AT2G19440.1	1.2e-203	73.00	O-Glycosyl hydrolases family 17 protein	[more]
AT3G04010.1	5.6e-190	70.61	O-Glycosyl hydrolases family 17 protein	[more]
AT5G18220.1	1.9e-185	68.13	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 366..449 e-value: 2.4E-38 score: 143.4
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 366..436 e-value: 9.4E-18 score: 64.7
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 22..352 e-value: 5.2E-109 score: 366.1
None	No IPR available	GENE3D	1.20.58.1040		coord: 363..461 e-value: 2.7E-9 score: 39.3
None	No IPR available	PANTHER	PTHR32227:SF95	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 8-RELATED	coord: 5..459
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 23..344 e-value: 7.7E-65 score: 219.5
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 260..273
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 5..459
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 23..344

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27692-RA	Carg27692-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds