Carg27324 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27324
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	calcium-binding protein CML39-like
Location	Carg_Chr09: 967552 .. 967821 (-)
RNA-Seq Expression	Carg27324
Synteny	Carg27324

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTTCTTCCGTCCAAAACTGAATTCGAAGAGCGCCTACTCAACGAAGGAACTGAAAGAGATCTTCCACCAGATGGATACGAATGAAGACGGCGGGTTGGGATTGTTCGAGATCAAGAACGCATTGAAGCAACTCGACGCTGAATTTCCAATCCATCTCGCCTACAAGGCTCTCTGTCACGCCGATCGGAACATAGATGGAAAGCTTGGCGAGGAGGAGATCGATGAATTTGTCAAGTACATGTTGAGCGAAGGCTATGTTGTATGA

mRNA sequence

ATGTCGTTCTTCCGTCCAAAACTGAATTCGAAGAGCGCCTACTCAACGAAGGAACTGAAAGAGATCTTCCACCAGATGGATACGAATGAAGACGGCGGGTTGGGATTGTTCGAGATCAAGAACGCATTGAAGCAACTCGACGCTGAATTTCCAATCCATCTCGCCTACAAGGCTCTCTGTCACGCCGATCGGAACATAGATGGAAAGCTTGGCGAGGAGGAGATCGATGAATTTGTCAAGTACATGTTGAGCGAAGGCTATGTTGTATGA

Coding sequence (CDS)

ATGTCGTTCTTCCGTCCAAAACTGAATTCGAAGAGCGCCTACTCAACGAAGGAACTGAAAGAGATCTTCCACCAGATGGATACGAATGAAGACGGCGGGTTGGGATTGTTCGAGATCAAGAACGCATTGAAGCAACTCGACGCTGAATTTCCAATCCATCTCGCCTACAAGGCTCTCTGTCACGCCGATCGGAACATAGATGGAAAGCTTGGCGAGGAGGAGATCGATGAATTTGTCAAGTACATGTTGAGCGAAGGCTATGTTGTATGA

Protein sequence

MSFFRPKLNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLGEEEIDEFVKYMLSEGYVV

Homology

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: KAG7024250.1 (hypothetical protein SDJN02_13064, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 183.7 bits (465), Expect = 6.8e-43
Identity = 89/89 (100.00%), Postives = 89/89 (100.00%), Query Frame = 0


Query: 1  MSFFRPKLNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALC 60
          MSFFRPKLNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALC
Sbjct: 1  MSFFRPKLNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALC 60

Query: 61 HADRNIDGKLGEEEIDEFVKYMLSEGYVV 90
          HADRNIDGKLGEEEIDEFVKYMLSEGYVV
Sbjct: 61 HADRNIDGKLGEEEIDEFVKYMLSEGYVV 89

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: KGN55187.1 (hypothetical protein Csa_012728 [Cucumis sativus])

HSP 1 Score: 148.7 bits (374), Expect = 2.4e-32
Identity = 72/95 (75.79%), Postives = 83/95 (87.37%), Query Frame = 0


Query: 2  SFFRPK-------LNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHL 61
          S FRPK       ++S+SAYS KEL++IF+QMDTNEDGGLGLFEIKNALK+LDAE P+HL
Sbjct: 3  SMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHL 62

Query: 62 AYKALCHADRNIDGKLGEEEIDEFVKYMLSEGYVV 90
          AY ALCHADRN DGKLGEEEID+FVKYM+S+GYVV
Sbjct: 63 AYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: KAA0064378.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa] >TYK20209.1 putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 143.3 bits (360), Expect = 1.0e-30
Identity = 66/82 (80.49%), Postives = 78/82 (95.12%), Query Frame = 0


Query: 8  LNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNID 67
          ++S+SAYS KEL++IF+QMDTNEDGGLGLFEIKNALK+LDAE P+HLAY ALC+ADRN D
Sbjct: 8  VSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCYADRNRD 67

Query: 68 GKLGEEEIDEFVKYMLSEGYVV 90
          GKLGEEEID+FVKYM+S+GYVV
Sbjct: 68 GKLGEEEIDQFVKYMMSKGYVV 89

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: KAG7037807.1 (hypothetical protein SDJN02_01438, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 138.7 bits (348), Expect = 2.5e-29
Identity = 63/80 (78.75%), Postives = 74/80 (92.50%), Query Frame = 0


Query: 10  SKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGK 69
           S+S YS KEL+EIF+QMDTNEDGGLGLFEIK ALKQLDAEFP+HLA+KALC +DRN DGK
Sbjct: 55  SESGYSAKELREIFNQMDTNEDGGLGLFEIKEALKQLDAEFPVHLAFKALCQSDRNRDGK 114

Query: 70  LGEEEIDEFVKYMLSEGYVV 90
           LG +EID+F+KY++SEGYVV
Sbjct: 115 LGRDEIDKFIKYLMSEGYVV 134

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: KAG6608479.1 (hypothetical protein SDJN03_01821, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 138.7 bits (348), Expect = 2.5e-29
Identity = 63/80 (78.75%), Postives = 74/80 (92.50%), Query Frame = 0


Query: 10  SKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGK 69
           S+S YS KEL+EIF+QMDTNEDGGLGLFEIK ALKQLDAEFP+HLA+KALC +DRN DGK
Sbjct: 55  SESGYSAKELREIFNQMDTNEDGGLGLFEIKEALKQLDAEFPVHLAFKALCQSDRNRDGK 114

Query: 70  LGEEEIDEFVKYMLSEGYVV 90
           LG +EID+F+KY++SEGYVV
Sbjct: 115 LGRDEIDKFIKYLMSEGYVV 134

[Close]

BLAST of Carg27324 vs. ExPASy TrEMBL
Match: A0A0A0L3J7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639750 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.2e-32
Identity = 72/95 (75.79%), Postives = 83/95 (87.37%), Query Frame = 0


Query: 2  SFFRPK-------LNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHL 61
          S FRPK       ++S+SAYS KEL++IF+QMDTNEDGGLGLFEIKNALK+LDAE P+HL
Sbjct: 3  SMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHL 62

Query: 62 AYKALCHADRNIDGKLGEEEIDEFVKYMLSEGYVV 90
          AY ALCHADRN DGKLGEEEID+FVKYM+S+GYVV
Sbjct: 63 AYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of Carg27324 vs. ExPASy TrEMBL
Match: A0A5D3D9N2 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003460 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.9e-31
Identity = 66/82 (80.49%), Postives = 78/82 (95.12%), Query Frame = 0


Query: 8  LNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNID 67
          ++S+SAYS KEL++IF+QMDTNEDGGLGLFEIKNALK+LDAE P+HLAY ALC+ADRN D
Sbjct: 8  VSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCYADRNRD 67

Query: 68 GKLGEEEIDEFVKYMLSEGYVV 90
          GKLGEEEID+FVKYM+S+GYVV
Sbjct: 68 GKLGEEEIDQFVKYMMSKGYVV 89

[Close]

BLAST of Carg27324 vs. ExPASy TrEMBL
Match: W9S022 (Putative calcium-binding protein CML10 OS=Morus notabilis OX=981085 GN=L484_000100 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.0e-09
Identity = 33/78 (42.31%), Postives = 46/78 (58.97%), Query Frame = 0


Query: 12  SAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLG 71
           + Y  ++LK +  + DT+ DG L   E+K A K+L A  P   A +ALCHAD+N DG + 
Sbjct: 23  AVYDKEKLKSVIRRYDTDGDGNLSRKELKKAFKELGATAPGWRAIRALCHADKNRDGFIS 82

Query: 72  EEEIDEFVKYMLSEGYVV 90
           EEE+   V Y    GYV+
Sbjct: 83  EEELANLVHYAAQRGYVL 100

[Close]

BLAST of Carg27324 vs. ExPASy TrEMBL
Match: A0A6A1UYX3 (Putative calcium-binding protein CML10 OS=Morella rubra OX=262757 GN=CJ030_MR0G017070 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.0e-09
Identity = 33/76 (43.42%), Postives = 48/76 (63.16%), Query Frame = 0


Query: 14  YSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLGEE 73
           Y+ ++L+ +F + DTN DG L   E++NA + L +  P   A++AL HADRN DG++ E 
Sbjct: 31  YTGEQLRGMFKRFDTNGDGQLSRQELRNAFRSLGSAAPGWRAFRALGHADRNGDGQISEA 90

Query: 74  EIDEFVKYMLSEGYVV 90
           E+D  V Y L  GY V
Sbjct: 91  ELDNLVGYALKHGYTV 106

[Close]

BLAST of Carg27324 vs. ExPASy TrEMBL
Match: A0A5C7H7G7 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_021561 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.0e-09
Identity = 31/70 (44.29%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 18  ELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLGEEEIDE 77
           ELK    + D N+DG L   E++ AL++LD+ FP   +++AL HAD N DG + E+E+D 
Sbjct: 137 ELKNWLRKFDENQDGHLSKEELEKALEELDSSFPAWRSWRALYHADENGDGYISEDELDG 196

Query: 78  FVKYMLSEGY 88
            VKY++  GY
Sbjct: 197 LVKYIIKRGY 206

The following BLAST results are available for this feature:

BLAST of Carg27324 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024250.1	6.8e-43	100.00	hypothetical protein SDJN02_13064, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KGN55187.1	2.4e-32	75.79	hypothetical protein Csa_012728 [Cucumis sativus]	[more]
KAA0064378.1	1.0e-30	80.49	putative calcium-binding protein CML10 [Cucumis melo var. makuwa] >TYK20209.1 pu...	[more]
KAG7037807.1	2.5e-29	78.75	hypothetical protein SDJN02_01438, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6608479.1	2.5e-29	78.75	hypothetical protein SDJN03_01821, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Carg27324 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg27324 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3J7	1.2e-32	75.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639750 PE=4 SV=1	[more]
A0A5D3D9N2	4.9e-31	80.49	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
W9S022	7.0e-09	42.31	Putative calcium-binding protein CML10 OS=Morus notabilis OX=981085 GN=L484_0001...	[more]
A0A6A1UYX3	7.0e-09	43.42	Putative calcium-binding protein CML10 OS=Morella rubra OX=262757 GN=CJ030_MR0G0...	[more]
A0A5C7H7G7	7.0e-09	44.29	Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_021561 PE=4 SV=1	[more]

BLAST of Carg27324 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 54..82 e-value: 59.0 score: 3.5 coord: 18..46 e-value: 0.11 score: 21.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 14..49 score: 11.779655
None	No IPR available	GENE3D	1.10.238.10		coord: 1..87 e-value: 1.4E-11 score: 46.3
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 8..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27324-RA	Carg27324-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding