Carg27297 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27297
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionDUF4149 domain-containing protein
LocationCarg_Chr15: 11744 .. 13199 (-)
RNA-Seq ExpressionCarg27297
SyntenyCarg27297
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCAACCTATTCGCTTTGTGTCTCGTCATTACTTCTTTAACTGCAGCGGGACTCTGGTCTCCTTCCCCGGCGTCCCGGCAAGATCACGAGCAGGATGTTATTGTTAAAGAAGGTCACCGAGTGGTTGTGGTCGAGTATGGCGACCAAGGTCAACACAATACTAAGGTTTCCATCTCTTCCGAACCCACCAAAGATGCCTCCCCTAGCAACCCACTACATGATAGTTTAAATATTGGGATCCCCAACGAAGACTCCGAAAGGCACCGCACCAGAGATCTTATTTGCGATGCCTTGGGCAAATGTAAGCATAAGATAGCCAGTGCTGTGGGGAAGGCTAAAGTAATGGTTTCGGAGACGGCGCAGGAGGCCCACGACGTTGGAGAGGCTGTTGCTGGTGCTTTCGATGAAGCCAAAGAGACAGTTTCAGACAAATCTCACCACGTGGGAACGTCGTTCTCAGAGAAAGGGCATCGATTGAGGGAGTCGGTTGAGAAAGCCAGAGAGGATGCCGACGAGTTCCTGGAGAAAACGAAAGAGACGGTTTTGGAGAAAGCACGTGACTTGAAAGAGGGTGCAAAGGATGTATTGAAGGAAGGCAAAGCACGTGACTTGAAAGAGGGTGCAATGGAGAAAGGGAGAGAAGCAAGACAAACTGCGGAGAAGATTAAAACTGGTGGAAACAAGGTCAAGGAGAATCTGATGGGTATTCCTGATGGAGGATTAAAGCTAGTAAATGATTCCTTTAGGTACTTGAGGTCGTTAGAGTCGTGGAAAGCGGCGATGGATGTGTTGAGTCTGTTGGGATTTGGTATGGCTTTGGGAATGGGCGTGTGGACTACCTTCATCTCTAGCTATGTGCTGGCGAGTGCGTTGCCAAGGCAGCAGTTGGCAGTGGTACAGAGCAAGATATATCCCCTGTATTTTAGGGCCATGGCTTCCAGCATTGGGATGGCCCTATTCGGGCATCTATTCAGCCGCACAAAATGGATGTTCCCAATTCCGAAAAATGCTGAAGTGGTCCAAGGATATGTACTTGTGGCTGCACTTTTGATGATTTTTGCCAATTCTCTCTACATGGAGCCCCAAGCCACCAAGGTATCTTCTGACCGCTGCTCCACCTTGAGGTTTTTTTAGTTTTGTACTAATTCCCTGTTATTGTGTATTTATTCCTGTGCAGGTAATGTTTGAGAGATTAAAAGTGGAGAAGGAAGAAGGAAAAGGAATTGAAGACATAGCCGCTGAACCTCGGGACGCCAGTGATAATCCCCCAGCAGTCACAACCAGCACAGCCACACAAGTCGTAGAACGAGAGGCGGTGAAGTCCAGAATCGTGGGGTTGAATAAGAGGCTGAAGAAGCTGAATTCGTGTTCATCCTTGTTAAACCTGCTCACTCTGATGGCTCTCACCTGGCATCTTGTGTACCTGAGCCAGCGTCTGTGCATCCCCTGCTAA

mRNA sequence

ATGCCCAACCTATTCGCTTTGTGTCTCGTCATTACTTCTTTAACTGCAGCGGGACTCTGGTCTCCTTCCCCGGCGTCCCGGCAAGATCACGAGCAGGATGTTATTGTTAAAGAAGGTCACCGAGTGGTTGTGGTCGAGTATGGCGACCAAGGTCAACACAATACTAAGGTTTCCATCTCTTCCGAACCCACCAAAGATGCCTCCCCTAGCAACCCACTACATGATAGTTTAAATATTGGGATCCCCAACGAAGACTCCGAAAGGCACCGCACCAGAGATCTTATTTGCGATGCCTTGGGCAAATGTAAGCATAAGATAGCCAGTGCTGTGGGGAAGGCTAAAGTAATGGTTTCGGAGACGGCGCAGGAGGCCCACGACGTTGGAGAGGCTGTTGCTGGTGCTTTCGATGAAGCCAAAGAGACAGTTTCAGACAAATCTCACCACGTGGGAACGTCGTTCTCAGAGAAAGGGCATCGATTGAGGGAGTCGGTTGAGAAAGCCAGAGAGGATGCCGACGAGTTCCTGGAGAAAACGAAAGAGACGGTTTTGGAGAAAGCACGTGACTTGAAAGAGGGTGCAAAGGATGTATTGAAGGAAGGCAAAGCACGTGACTTGAAAGAGGGTGCAATGGAGAAAGGGAGAGAAGCAAGACAAACTGCGGAGAAGATTAAAACTGGTGGAAACAAGGTCAAGGAGAATCTGATGGGTATTCCTGATGGAGGATTAAAGCTAGTAAATGATTCCTTTAGGTACTTGAGGTCGTTAGAGTCGTGGAAAGCGGCGATGGATGTGTTGAGTCTGTTGGGATTTGGTATGGCTTTGGGAATGGGCGTGTGGACTACCTTCATCTCTAGCTATGTGCTGGCGAGTGCGTTGCCAAGGCAGCAGTTGGCAGTGGTACAGAGCAAGATATATCCCCTGTATTTTAGGGCCATGGCTTCCAGCATTGGGATGGCCCTATTCGGGCATCTATTCAGCCGCACAAAATGGATGTTCCCAATTCCGAAAAATGCTGAAGTGGTCCAAGGATATGTACTTGTGGCTGCACTTTTGATGATTTTTGCCAATTCTCTCTACATGGAGCCCCAAGCCACCAAGGTAATGTTTGAGAGATTAAAAGTGGAGAAGGAAGAAGGAAAAGGAATTGAAGACATAGCCGCTGAACCTCGGGACGCCAGTGATAATCCCCCAGCAGTCACAACCAGCACAGCCACACAAGTCGTAGAACGAGAGGCGGTGAAGTCCAGAATCGTGGGGTTGAATAAGAGGCTGAAGAAGCTGAATTCGTGTTCATCCTTGTTAAACCTGCTCACTCTGATGGCTCTCACCTGGCATCTTGTGTACCTGAGCCAGCGTCTGTGCATCCCCTGCTAA

Coding sequence (CDS)

ATGCCCAACCTATTCGCTTTGTGTCTCGTCATTACTTCTTTAACTGCAGCGGGACTCTGGTCTCCTTCCCCGGCGTCCCGGCAAGATCACGAGCAGGATGTTATTGTTAAAGAAGGTCACCGAGTGGTTGTGGTCGAGTATGGCGACCAAGGTCAACACAATACTAAGGTTTCCATCTCTTCCGAACCCACCAAAGATGCCTCCCCTAGCAACCCACTACATGATAGTTTAAATATTGGGATCCCCAACGAAGACTCCGAAAGGCACCGCACCAGAGATCTTATTTGCGATGCCTTGGGCAAATGTAAGCATAAGATAGCCAGTGCTGTGGGGAAGGCTAAAGTAATGGTTTCGGAGACGGCGCAGGAGGCCCACGACGTTGGAGAGGCTGTTGCTGGTGCTTTCGATGAAGCCAAAGAGACAGTTTCAGACAAATCTCACCACGTGGGAACGTCGTTCTCAGAGAAAGGGCATCGATTGAGGGAGTCGGTTGAGAAAGCCAGAGAGGATGCCGACGAGTTCCTGGAGAAAACGAAAGAGACGGTTTTGGAGAAAGCACGTGACTTGAAAGAGGGTGCAAAGGATGTATTGAAGGAAGGCAAAGCACGTGACTTGAAAGAGGGTGCAATGGAGAAAGGGAGAGAAGCAAGACAAACTGCGGAGAAGATTAAAACTGGTGGAAACAAGGTCAAGGAGAATCTGATGGGTATTCCTGATGGAGGATTAAAGCTAGTAAATGATTCCTTTAGGTACTTGAGGTCGTTAGAGTCGTGGAAAGCGGCGATGGATGTGTTGAGTCTGTTGGGATTTGGTATGGCTTTGGGAATGGGCGTGTGGACTACCTTCATCTCTAGCTATGTGCTGGCGAGTGCGTTGCCAAGGCAGCAGTTGGCAGTGGTACAGAGCAAGATATATCCCCTGTATTTTAGGGCCATGGCTTCCAGCATTGGGATGGCCCTATTCGGGCATCTATTCAGCCGCACAAAATGGATGTTCCCAATTCCGAAAAATGCTGAAGTGGTCCAAGGATATGTACTTGTGGCTGCACTTTTGATGATTTTTGCCAATTCTCTCTACATGGAGCCCCAAGCCACCAAGGTAATGTTTGAGAGATTAAAAGTGGAGAAGGAAGAAGGAAAAGGAATTGAAGACATAGCCGCTGAACCTCGGGACGCCAGTGATAATCCCCCAGCAGTCACAACCAGCACAGCCACACAAGTCGTAGAACGAGAGGCGGTGAAGTCCAGAATCGTGGGGTTGAATAAGAGGCTGAAGAAGCTGAATTCGTGTTCATCCTTGTTAAACCTGCTCACTCTGATGGCTCTCACCTGGCATCTTGTGTACCTGAGCCAGCGTCTGTGCATCCCCTGCTAA

Protein sequence

MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSISSEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSETAQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKETVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDGGLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVVQSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGLNKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC
Homology
BLAST of Carg27297 vs. NCBI nr
Match: KAG7015871.1 (Transmembrane protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 870.5 bits (2248), Expect = 6.2e-249
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS
Sbjct: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
           SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET
Sbjct: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180
           AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE
Sbjct: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180

Query: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240
           TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG
Sbjct: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240

Query: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300
           GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV
Sbjct: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300

Query: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360
           QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM
Sbjct: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360

Query: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGL 420
           EPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGL
Sbjct: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGL 420

Query: 421 NKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           NKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC
Sbjct: 421 NKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 457

BLAST of Carg27297 vs. NCBI nr
Match: XP_022938890.1 (uncharacterized protein LOC111444965 [Cucurbita moschata] >XP_023550066.1 uncharacterized protein LOC111808367 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023550068.1 uncharacterized protein LOC111808367 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865.1 bits (2234), Expect = 2.6e-247
Identity = 455/457 (99.56%), Postives = 456/457 (99.78%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS
Sbjct: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
           SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET
Sbjct: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180
           AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE
Sbjct: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180

Query: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240
           TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG
Sbjct: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240

Query: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300
           GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV
Sbjct: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300

Query: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360
           QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM
Sbjct: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360

Query: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGL 420
           EPQATKVMFERLKVEKEEGKGIEDIAAEPRDA+DNPPAVTTSTATQVVEREAVKSRIVGL
Sbjct: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDANDNPPAVTTSTATQVVEREAVKSRIVGL 420

Query: 421 NKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           NKRLKKLNS SSLLNLLTLMALTWHLVYLSQRLCIPC
Sbjct: 421 NKRLKKLNSYSSLLNLLTLMALTWHLVYLSQRLCIPC 457

BLAST of Carg27297 vs. NCBI nr
Match: XP_023550065.1 (uncharacterized protein LOC111808367 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 854.7 bits (2207), Expect = 3.5e-244
Identity = 455/473 (96.19%), Postives = 456/473 (96.41%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS
Sbjct: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
           SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET
Sbjct: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180
           AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE
Sbjct: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180

Query: 181 TVLE----------------KARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIK 240
           TVLE                KARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIK
Sbjct: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIK 240

Query: 241 TGGNKVKENLMGIPDGGLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFIS 300
           TGGNKVKENLMGIPDGGLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFIS
Sbjct: 241 TGGNKVKENLMGIPDGGLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFIS 300

Query: 301 SYVLASALPRQQLAVVQSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGY 360
           SYVLASALPRQQLAVVQSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGY
Sbjct: 301 SYVLASALPRQQLAVVQSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGY 360

Query: 361 VLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTA 420
           VLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKGIEDIAAEPRDA+DNPPAVTTSTA
Sbjct: 361 VLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKGIEDIAAEPRDANDNPPAVTTSTA 420

Query: 421 TQVVEREAVKSRIVGLNKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           TQVVEREAVKSRIVGLNKRLKKLNS SSLLNLLTLMALTWHLVYLSQRLCIPC
Sbjct: 421 TQVVEREAVKSRIVGLNKRLKKLNSYSSLLNLLTLMALTWHLVYLSQRLCIPC 473

BLAST of Carg27297 vs. NCBI nr
Match: KAG6578300.1 (Transmembrane protein 205, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 844.0 bits (2179), Expect = 6.2e-241
Identity = 446/452 (98.67%), Postives = 448/452 (99.12%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           M NLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS
Sbjct: 1   MTNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
           SEPTKDASPSN LHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET
Sbjct: 61  SEPTKDASPSNRLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180
           AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE
Sbjct: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180

Query: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240
           TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG
Sbjct: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240

Query: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300
           GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV
Sbjct: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300

Query: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360
           QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM
Sbjct: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360

Query: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGL 420
           EPQATKVMFERLKVEKEEGKGIEDIAAEPR A+DNPPA+TTSTATQVVEREAVKSRIVGL
Sbjct: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRHANDNPPAITTSTATQVVEREAVKSRIVGL 420

Query: 421 NKRLKKLNSCSSLLNLLTLMALTWHLVYLSQR 453
           NKRLKKLNS SSLLNLLTLMALTWHLVYLSQR
Sbjct: 421 NKRLKKLNSYSSLLNLLTLMALTWHLVYLSQR 452

BLAST of Carg27297 vs. NCBI nr
Match: XP_022992882.1 (uncharacterized protein LOC111489083 [Cucurbita maxima])

HSP 1 Score: 843.6 bits (2178), Expect = 8.1e-241
Identity = 443/457 (96.94%), Postives = 450/457 (98.47%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           MPNLFALCLVITSLTAAGLWSPSPASR DHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS
Sbjct: 1   MPNLFALCLVITSLTAAGLWSPSPASRLDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
           SEPTKDASPSNPLHDSLN+GIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET
Sbjct: 61  SEPTKDASPSNPLHDSLNVGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180
           AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE
Sbjct: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180

Query: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240
           TVLEKARDLKEGAKDVLKEGKAR+L+EGAMEKGREARQTAEKIKTGGNKVKENL+GIP G
Sbjct: 181 TVLEKARDLKEGAKDVLKEGKARELREGAMEKGREARQTAEKIKTGGNKVKENLIGIPGG 240

Query: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300
           GLKLVNDSFRYL SLESWKAAMDVLSLLGF MALGMGVWTTFISSYVLASALPRQQLAVV
Sbjct: 241 GLKLVNDSFRYLGSLESWKAAMDVLSLLGFSMALGMGVWTTFISSYVLASALPRQQLAVV 300

Query: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360
           QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALL IFANSLYM
Sbjct: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLTIFANSLYM 360

Query: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGL 420
           EPQATKVMFERLKVEKEEGKGIEDIAAEPR A+DNPPA+TTSTATQVV+REAVKSRIVGL
Sbjct: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRHANDNPPAITTSTATQVVDREAVKSRIVGL 420

Query: 421 NKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           NKRLKKLNS SSLLNLLTLMALTWHLVYLSQRLCIPC
Sbjct: 421 NKRLKKLNSYSSLLNLLTLMALTWHLVYLSQRLCIPC 457

BLAST of Carg27297 vs. ExPASy Swiss-Prot
Match: Q6UW68 (Transmembrane protein 205 OS=Homo sapiens OX=9606 GN=TMEM205 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.6e-08
Identity = 40/120 (33.33%), Postives = 62/120 (51.67%), Query Frame = 0

Query: 264 VLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVVQSKIYPLYFRAMASSIGMALFGH 323
           ++ LL    A GM +W TF+S ++L  +LPR    +VQSK++P YF     S+G A    
Sbjct: 13  MVHLLVLSGAWGMQMWVTFVSGFLLFRSLPRHTFGLVQSKLFPFYFHI---SMGCAFINL 72

Query: 324 LFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKGIE 383
               ++  +      E  Q Y+L  +L +   N+ ++EP+ T  M+    VEKE G G E
Sbjct: 73  CILASQHAWAQLTFWEASQLYLLFLSLTLATVNARWLEPRTTAAMWALQTVEKERGLGGE 129

BLAST of Carg27297 vs. ExPASy Swiss-Prot
Match: Q5REM8 (Transmembrane protein 205 OS=Pongo abelii OX=9601 GN=TMEM205 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.4e-08
Identity = 40/120 (33.33%), Postives = 62/120 (51.67%), Query Frame = 0

Query: 264 VLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVVQSKIYPLYFRAMASSIGMALFGH 323
           ++ LL    A GM +W TF+S ++L  +LPR    +VQSK++P YF     S+G A    
Sbjct: 13  MVHLLVLSGAWGMQMWVTFVSGFLLFRSLPRHTFGLVQSKLFPFYFHI---SMGCAFVNL 72

Query: 324 LFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKGIE 383
               ++  +      E  Q Y+L  +L +   N+ ++EP+ T  M+    VEKE G G E
Sbjct: 73  CILASQHAWAQLTFWEASQLYLLFLSLTLATVNARWLEPRTTAAMWALQTVEKERGLGGE 129

BLAST of Carg27297 vs. ExPASy Swiss-Prot
Match: Q91XE8 (Transmembrane protein 205 OS=Mus musculus OX=10090 GN=Tmem205 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.5e-08
Identity = 42/120 (35.00%), Postives = 62/120 (51.67%), Query Frame = 0

Query: 264 VLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVVQSKIYPLYFRAMASSIGMALFGH 323
           V+ LL    A GM VW TFIS ++L  +LPR    +VQSK++P+YF     S+G A    
Sbjct: 13  VIHLLVLSGAWGMQVWVTFISGFLLFRSLPRHTFGLVQSKVFPVYFHV---SLGCAFINL 72

Query: 324 LFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKGIE 383
                +  +      EV Q  +L+ +L +   N+ ++E + T VM     +EKE G G E
Sbjct: 73  CILAPQRAWIHLTLWEVSQLSLLLLSLTLATINARWLEARTTAVMRALQSIEKERGLGTE 129

BLAST of Carg27297 vs. ExPASy Swiss-Prot
Match: Q32L10 (Transmembrane protein 205 OS=Bos taurus OX=9913 GN=TMEM205 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-07
Identity = 41/120 (34.17%), Postives = 61/120 (50.83%), Query Frame = 0

Query: 264 VLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVVQSKIYPLYFRAMASSIGMALFGH 323
           V+ LL    A GM +W TFIS +VL   LPR    +VQSK++P YF     S+G A    
Sbjct: 13  VVHLLVLSGAWGMQMWVTFISGFVLFRGLPRHTFGLVQSKLFPFYFHI---SMGCAFVNL 72

Query: 324 LFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKGIE 383
               ++  +      E  Q ++L+ +L +   N+ ++E + T  M+    VEKE G G E
Sbjct: 73  CILASQCSWAQLTFWEASQLFLLLLSLTLATINARWLESRTTAAMWALQTVEKERGLGGE 129

BLAST of Carg27297 vs. ExPASy Swiss-Prot
Match: Q28GF8 (Transmembrane protein 205 OS=Xenopus tropicalis OX=8364 GN=tmem205 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 7.2e-06
Identity = 51/189 (26.98%), Postives = 83/189 (43.92%), Query Frame = 0

Query: 265 LSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVVQSKIYPLYFRAM--ASSIGMALFG 324
           + LL    + GM  WTTF++ +VL   +PR    +VQSK++P Y   +   S I +A++ 
Sbjct: 14  IHLLVLSASWGMQCWTTFVAGFVLIRGVPRHTFGLVQSKLFPFYNHIVLCCSFISLAIYA 73

Query: 325 HLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKGI 384
               R      +   +E VQ  +    LL    ++ +  P  TK MF+   +E+E   G 
Sbjct: 74  AYHPR-----ELLSPSESVQITLFFVCLLAAALHARWFSPVTTKTMFKMHIIEREHSLG- 133

Query: 385 EDIAAEPRDASDNPPAVTTSTATQVVEREAVKS-RIVGLNKRLKKLNSCSSLLNLLTLMA 444
                           V  S   +  +R   K  +   L +R  + +  SSL NLL L+ 
Sbjct: 134 --------------QGVGLSANREAYQRLQEKDPKYKALRQRFMRYHGISSLCNLLCLLC 182

Query: 445 LTWHLVYLS 451
              +LVY++
Sbjct: 194 NGANLVYMA 182

BLAST of Carg27297 vs. ExPASy TrEMBL
Match: A0A6J1FL32 (uncharacterized protein LOC111444965 OS=Cucurbita moschata OX=3662 GN=LOC111444965 PE=4 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.3e-247
Identity = 455/457 (99.56%), Postives = 456/457 (99.78%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS
Sbjct: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
           SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET
Sbjct: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180
           AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE
Sbjct: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180

Query: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240
           TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG
Sbjct: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240

Query: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300
           GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV
Sbjct: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300

Query: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360
           QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM
Sbjct: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360

Query: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGL 420
           EPQATKVMFERLKVEKEEGKGIEDIAAEPRDA+DNPPAVTTSTATQVVEREAVKSRIVGL
Sbjct: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDANDNPPAVTTSTATQVVEREAVKSRIVGL 420

Query: 421 NKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           NKRLKKLNS SSLLNLLTLMALTWHLVYLSQRLCIPC
Sbjct: 421 NKRLKKLNSYSSLLNLLTLMALTWHLVYLSQRLCIPC 457

BLAST of Carg27297 vs. ExPASy TrEMBL
Match: A0A6J1JR64 (uncharacterized protein LOC111489083 OS=Cucurbita maxima OX=3661 GN=LOC111489083 PE=4 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.9e-241
Identity = 443/457 (96.94%), Postives = 450/457 (98.47%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           MPNLFALCLVITSLTAAGLWSPSPASR DHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS
Sbjct: 1   MPNLFALCLVITSLTAAGLWSPSPASRLDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
           SEPTKDASPSNPLHDSLN+GIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET
Sbjct: 61  SEPTKDASPSNPLHDSLNVGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180
           AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE
Sbjct: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180

Query: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240
           TVLEKARDLKEGAKDVLKEGKAR+L+EGAMEKGREARQTAEKIKTGGNKVKENL+GIP G
Sbjct: 181 TVLEKARDLKEGAKDVLKEGKARELREGAMEKGREARQTAEKIKTGGNKVKENLIGIPGG 240

Query: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300
           GLKLVNDSFRYL SLESWKAAMDVLSLLGF MALGMGVWTTFISSYVLASALPRQQLAVV
Sbjct: 241 GLKLVNDSFRYLGSLESWKAAMDVLSLLGFSMALGMGVWTTFISSYVLASALPRQQLAVV 300

Query: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360
           QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALL IFANSLYM
Sbjct: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLTIFANSLYM 360

Query: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGL 420
           EPQATKVMFERLKVEKEEGKGIEDIAAEPR A+DNPPA+TTSTATQVV+REAVKSRIVGL
Sbjct: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRHANDNPPAITTSTATQVVDREAVKSRIVGL 420

Query: 421 NKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           NKRLKKLNS SSLLNLLTLMALTWHLVYLSQRLCIPC
Sbjct: 421 NKRLKKLNSYSSLLNLLTLMALTWHLVYLSQRLCIPC 457

BLAST of Carg27297 vs. ExPASy TrEMBL
Match: A0A6J1DI19 (uncharacterized protein LOC111020246 OS=Momordica charantia OX=3673 GN=LOC111020246 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 9.3e-150
Identity = 318/457 (69.58%), Postives = 355/457 (77.68%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           M NLFALC+++TSLTAAGLWSPSPAS+QDHEQDVIVKEGHRVVVVEYGDQG+ +TKVSIS
Sbjct: 1   MTNLFALCVIVTSLTAAGLWSPSPASKQDHEQDVIVKEGHRVVVVEYGDQGRQSTKVSIS 60

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
            EP    S  NPLH+       + ++ERHR+RDLICDA GKCKHKIA+AVGKAKVMVSET
Sbjct: 61  PEP---ESKPNPLHNP--SASQDSETERHRSRDLICDAFGKCKHKIANAVGKAKVMVSET 120

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180
           A+   DVGEAVA AFDEAK+TVSDKS           HR++ESVEKARE A+ F+EKTK+
Sbjct: 121 AE---DVGEAVADAFDEAKDTVSDKS-----------HRVKESVEKAREGANGFVEKTKD 180

Query: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIKTGGNKVKENLMGIPDG 240
           TV  K  DLKEGAK+  KE K R   EG +E     RQTAE+IK GGNK    LM I   
Sbjct: 181 TVSGKVHDLKEGAKENAKEVKGRG--EGVVEDSE--RQTAERIKAGGNK----LMVILGR 240

Query: 241 GLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVV 300
           G+KLV  S RYL S ES ++ M VLSLLGF MALGMGVWTTFISSYVLA ALPRQQL VV
Sbjct: 241 GVKLVKGSVRYLGSAESLRSVMGVLSLLGFAMALGMGVWTTFISSYVLAIALPRQQLGVV 300

Query: 301 QSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYM 360
           QSKIYP+YFRAMASSIGMAL GHLFS +         A+VVQGYVL+AALLM FAN +YM
Sbjct: 301 QSKIYPVYFRAMASSIGMALLGHLFSHSNTY----TKAQVVQGYVLLAALLMTFANYIYM 360

Query: 361 EPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGL 420
           EPQATKVMFERLK+EKEEG+GIEDI A+P   +       T  AT+VV+RE VKSRIVGL
Sbjct: 361 EPQATKVMFERLKMEKEEGRGIEDITADPAITTSKAAMSATLQATEVVDREVVKSRIVGL 420

Query: 421 NKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           N+RLKKLNS SSLLNLLTLMALTWHLVYLSQRL I C
Sbjct: 421 NQRLKKLNSYSSLLNLLTLMALTWHLVYLSQRLRISC 426

BLAST of Carg27297 vs. ExPASy TrEMBL
Match: A0A1S3CA56 (uncharacterized protein LOC103498695 OS=Cucumis melo OX=3656 GN=LOC103498695 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 2.4e-145
Identity = 304/460 (66.09%), Postives = 349/460 (75.87%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           M NLFA+ L+IT+LTAAGLWSP P       Q+VIVKEGHRVVVVEY DQGQHNTKVSIS
Sbjct: 17  MTNLFAMFLIITTLTAAGLWSPPPP-----PQNVIVKEGHRVVVVEYDDQGQHNTKVSIS 76

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
           SEP  DA                ++SERHRT+DLICD  GKCKHK+ASAV KAKVMV+ET
Sbjct: 77  SEPDLDA----------------KNSERHRTKDLICDVYGKCKHKVASAVEKAKVMVTET 136

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFLEKTKE 180
           AQEAHDVGE+VAGAFDEAK+ + + +     +F E   +L+E  + A+E   E  +K   
Sbjct: 137 AQEAHDVGESVAGAFDEAKDKLKEGAKE---TFGEAKDKLKEGAKGAKETFGEAKDK--- 196

Query: 181 TVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGRE--ARQTAEKIKTGGNKVKENLMGIP 240
                   LKEGAK+ L+  K+R+ K   + KG E  A++T EKI+TG NK+KENLMG+ 
Sbjct: 197 --------LKEGAKETLEMAKSREEK---VVKGAERVAKETGEKIQTGENKLKENLMGLV 256

Query: 241 DGGLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLA 300
           D G K++N  FR+L         MD L LLGF MALGMGVW TFISSYVLAS LPRQQL 
Sbjct: 257 DRGFKVMNYLFRHL------GVGMDALGLLGFAMALGMGVWVTFISSYVLASVLPRQQLG 316

Query: 301 VVQSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSL 360
           VVQSKIYP+YF+AMAS IGMAL GHLFSRT+W FPIPKN+EVVQGYVLVAALLMIFANSL
Sbjct: 317 VVQSKIYPVYFKAMASCIGMALLGHLFSRTEWKFPIPKNSEVVQGYVLVAALLMIFANSL 376

Query: 361 YMEPQATKVMFERLKVEKEEGKGIEDIAAEPR-DASDNPPAVTTSTATQVVEREAVKSRI 420
           YMEP+ATKVMFERLK+EKEEG+GIEDI  E   +  DN PA+T+ST TQ+V+RE VKSRI
Sbjct: 377 YMEPRATKVMFERLKIEKEEGRGIEDIGREETVNVIDNSPAITSSTPTQIVDREVVKSRI 432

Query: 421 VGLNKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           VGLNKRLKKLNS SSLLNLLTLMALTWHLVYLSQRLC PC
Sbjct: 437 VGLNKRLKKLNSYSSLLNLLTLMALTWHLVYLSQRLCNPC 432

BLAST of Carg27297 vs. ExPASy TrEMBL
Match: A0A5D3BMZ0 (DUF4149 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00490 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 2.0e-144
Identity = 304/461 (65.94%), Postives = 347/461 (75.27%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           M NLFA+ L++T+LTAAGLWSP P       Q+VIVKEGHRVVVVEY DQGQHNTKVSIS
Sbjct: 1   MTNLFAMFLIVTTLTAAGLWSPPPP-----PQNVIVKEGHRVVVVEYDDQGQHNTKVSIS 60

Query: 61  SEPTKDASPSNPLHDSLNIGIPNEDSERHRTRDLICDALGKCKHKIASAVGKAKVMVSET 120
           SEP  DA                + SERHRT+DLICD  GKCKHK+ASAV KAKVMV+ET
Sbjct: 61  SEPDLDA----------------KISERHRTKDLICDVYGKCKHKVASAVEKAKVMVTET 120

Query: 121 AQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDA-DEFLEKTK 180
           AQEAHDVGE+VAGAFDEAK+                  +L+E  ++  ++A D+  E  K
Sbjct: 121 AQEAHDVGESVAGAFDEAKD------------------KLKEGAKETFDEAKDKLKEGAK 180

Query: 181 ETVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGRE--ARQTAEKIKTGGNKVKENLMGI 240
           ET  E    LKEGAK+ L+  K+R+ K   + KG E  A++T EKI+TG NK+KENLMG+
Sbjct: 181 ETFGEAKDKLKEGAKETLEMAKSREEK---VVKGAERVAKETGEKIQTGENKLKENLMGL 240

Query: 241 PDGGLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQL 300
            D G K++N  FR+L         MD L LLGF MALGMGVW TFISSYVLAS LPRQQL
Sbjct: 241 VDRGFKVMNYLFRHL------GVGMDALGLLGFAMALGMGVWVTFISSYVLASVLPRQQL 300

Query: 301 AVVQSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANS 360
            VVQSKIYP+YF+AMAS IGMAL GHLFSRT+W FPIPKN+EVVQGYVLVAALLMIFANS
Sbjct: 301 GVVQSKIYPVYFKAMASCIGMALLGHLFSRTEWKFPIPKNSEVVQGYVLVAALLMIFANS 360

Query: 361 LYMEPQATKVMFERLKVEKEEGKGIEDIAAEPR-DASDNPPAVTTSTATQVVEREAVKSR 420
           LYMEP+ATKVMFERLK+EKEEG+GIEDI  E   +  DN PA+T+ST TQ+V+RE VKSR
Sbjct: 361 LYMEPRATKVMFERLKIEKEEGRGIEDIGREETVNVIDNSPAITSSTPTQIVDREVVKSR 413

Query: 421 IVGLNKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           IVGLNKRLKKLNS SSLLNLLTLMALTWHLVYLSQRLC PC
Sbjct: 421 IVGLNKRLKKLNSYSSLLNLLTLMALTWHLVYLSQRLCNPC 413

BLAST of Carg27297 vs. TAIR 10
Match: AT1G72100.1 (late embryogenesis abundant domain-containing protein / LEA domain-containing protein )

HSP 1 Score: 254.2 bits (648), Expect = 2.0e-67
Identity = 196/512 (38.28%), Postives = 271/512 (52.93%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDH-----EQDVIVKEGHRVVVVEYGDQGQHNT 60
           M NL ALCLV+++L AA +WSPSPA    +     E +VIVK+GH VVVVEY   G+ NT
Sbjct: 1   MTNLLALCLVLSTLLAAEVWSPSPAMTTHNTAVASEGEVIVKDGHHVVVVEYDRDGKTNT 60

Query: 61  KVSIS------------------------SEPTKDASPSNPLHDSLNIGIP---NEDSER 120
           +VSIS                         E  K+ +   P H    I  P   +E  + 
Sbjct: 61  RVSISPPSADQGEEKENEVEMGTSMFRNVKEKAKETASYLP-HVGQGISQPVMTDEARDH 120

Query: 121 HRTR-DLICDALGKCKHKIASAVGKAK----VMVSETAQE--------AHDVGEAVAGAF 180
           H T  ++ICDA GKC+ KIAS VG+AK      V ETA +        AHDV E V  A 
Sbjct: 121 HATAGEVICDAFGKCRQKIASVVGRAKDRTVDSVGETASDVREAAAHKAHDVKETVTHAA 180

Query: 181 DEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDADEFL----EKTKETVLEKARDLKE 240
            + ++TV+D++ +     +EK H  +E V     DA E +       KE+V +KA D KE
Sbjct: 181 RDVEDTVADQAQYAKGRVTEKAHDPKEGVAHKAHDAKESVADKAHDAKESVAQKAHDAKE 240

Query: 241 GAKDVLKEGKARDLKEGAMEKGREARQTA-EKIKTGGNKVKENLMGIPDGGLKLVNDSFR 300
             ++     KA D+KE   +K  E+++ A ++++    ++KE          + V +  R
Sbjct: 241 KVRE-----KAHDVKETVAQKAHESKERAKDRVREKAQELKETATHKSKNAWERVKNGAR 300

Query: 301 YLRS-----LESWKAAMDVLSLLGFGMALGMGVWTTFISSYVLASALPRQQLAVVQSKIY 360
              S     L   K A  ++ L G   A G  VW TF+SSYVLAS L RQQ  VVQSK+Y
Sbjct: 301 EFGSATAATLSPTKVA-SIVGLTGIAAAFGTSVWVTFVSSYVLASVLGRQQFGVVQSKLY 360

Query: 361 PLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVAALLMIFANSLYMEPQAT 420
           P+YF+A +  I + LFGH+ SR + +  +    E+ QG  L+++  MI AN  ++EP+AT
Sbjct: 361 PVYFKATSVGILVGLFGHVLSRRRKL--LTDATEMWQGVNLLSSFFMIEANKSFVEPRAT 420

Query: 421 KVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGLNKRLK 458
           K MFER+K EKEEG+G E                   T+ Q + R     ++  L++RL 
Sbjct: 421 KAMFERMKAEKEEGRGGE------------------RTSEQELRR-----KLEQLSERLS 480

BLAST of Carg27297 vs. TAIR 10
Match: AT1G22600.1 (Late embryogenesis abundant protein (LEA) family protein )

HSP 1 Score: 182.6 bits (462), Expect = 7.3e-46
Identity = 150/469 (31.98%), Postives = 232/469 (49.47%), Query Frame = 0

Query: 1   MPNLFALCLVITSLTAAGLWSPSPASRQDHEQDVIVKEGHRVVVVEYGDQGQHNTKVSIS 60
           M  L AL LV+++L   G  + S       E D+IV++GHRVVVVEY   G+ NT+V IS
Sbjct: 1   MTKLLALSLVLSALFTVGHCTGS-------EDDIIVRDGHRVVVVEYDRDGETNTRVLIS 60

Query: 61  ------SEPTKDASP--SNPLHDSLNIGIPNEDSERHRT-RDLICDALGKCKHKIASAVG 120
                  + ++D      N   ++ +     E+ E H T  +LICDA+GKCKHK+ + +G
Sbjct: 61  PPGKEVDQKSRDEGEVFGNEKRETASSLPEEEEREHHATPGELICDAIGKCKHKLGTVLG 120

Query: 121 KAKVMVSETAQEAHDVGEAVAGAFDEAKETVSDKSHHVGTSFSEKGHRLRESVEKAREDA 180
           + K                     D     +SD++  +  +        RE++E      
Sbjct: 121 RVK---------------------DRTASDLSDETPEMTVA--------REALE------ 180

Query: 181 DEFLEKTKETVLEKARDLKEGAKDVLKEGKARDLKEGAMEKGREARQTAEKIK---TGGN 240
                  +E V  KAR+ +         GK   + E A +K    ++  EK++    G  
Sbjct: 181 ------VEEKVSWKAREAR---------GK---VNERATKKAHRVQKVLEKVQIAVRGIG 240

Query: 241 KVKENLMGIPDGGLKLVNDSFRYLRSLESWKAAMDVLSLLGFGMALGMGVWTTFISSYVL 300
            V    +G+   G                      V+ ++G   A GM VW TF+S YVL
Sbjct: 241 TVVATALGLTKIG---------------------SVVGIVGIAAAYGMCVWVTFVSGYVL 300

Query: 301 ASALPRQQLAVVQSKIYPLYFRAMASSIGMALFGHLFSRTKWMFPIPKNAEVVQGYVLVA 360
           AS L  QQ  VVQSK+YP+YF+A++  I + L GH+  R + +F      ++ Q   L++
Sbjct: 301 ASVLGEQQFGVVQSKMYPVYFKAVSVGILVGLLGHVIGRRRKVF--TDAVDMWQSVNLLS 360

Query: 361 ALLMIFANSLYMEPQATKVMFERLKVEKEEGKGIEDIAAEPRDASDNPPAVTTSTATQVV 420
           ++LM+ AN+ ++  +ATK MFE +K EKE+G+G  D + + + +            T+  
Sbjct: 361 SILMVEANASFVYTRATKAMFELIKAEKEDGRGF-DTSDQSQSSESAGRTRGKKKVTEKT 385

Query: 421 EREAVKSRIVGLNKRLKKLNSCSSLLNLLTLMALTWHLVYLSQRLCIPC 458
           + + VK R+  L++R++KLN+ SS LNLLTLM+LTWH VYL  RL + C
Sbjct: 421 DEDVVKQRLTKLSERMRKLNAYSSRLNLLTLMSLTWHFVYLGYRLSLTC 385

BLAST of Carg27297 vs. TAIR 10
Match: AT3G62580.1 (Late embryogenesis abundant protein (LEA) family protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-09
Identity = 53/192 (27.60%), Postives = 88/192 (45.83%), Query Frame = 0

Query: 267 LLGFGMALGMGVWTTFISSYVLASALPRQQLAVVQSKIYPLYFRAMAS--SIGMALFGHL 326
           LL F  A G  +W TFI   ++   LPR Q   +QSK++P YF  + S  +I ++ FG+L
Sbjct: 46  LLSFATAWGAALWATFIGGIIMFKNLPRHQFGNLQSKLFPAYFTLVGSCCAISLSAFGYL 105

Query: 327 FSRTKWMFPIPKNAEVVQGY---VLVAALLMIFANSLYMEPQATKVMFERLKVEKEEGKG 386
                W     K++  V+ Y    L++A      N     P    +M +R KVE+E   G
Sbjct: 106 H---PW-----KSSSTVEKYQIGFLLSAFAFNLTNLFVFTPMTIDMMKQRHKVERENNIG 165

Query: 387 IEDIAAEPRDASDNPPAVTTSTATQVVEREAVKSRIVGLNKRLKKLNSCSSLLNLLTLMA 446
            E   ++ R+ + + P                  ++  +NK+   ++  SSL N+ +  +
Sbjct: 166 DEVGWSKNREKAKSIP------------------KLAAMNKKFGMIHGLSSLANIFSFGS 211

Query: 447 LTWHLVYLSQRL 454
           L  H  YL+ +L
Sbjct: 226 LAMHSWYLAGKL 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015871.16.2e-249100.00Transmembrane protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022938890.12.6e-24799.56uncharacterized protein LOC111444965 [Cucurbita moschata] >XP_023550066.1 unchar... [more]
XP_023550065.13.5e-24496.19uncharacterized protein LOC111808367 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6578300.16.2e-24198.67Transmembrane protein 205, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022992882.18.1e-24196.94uncharacterized protein LOC111489083 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q6UW682.6e-0833.33Transmembrane protein 205 OS=Homo sapiens OX=9606 GN=TMEM205 PE=1 SV=1[more]
Q5REM83.4e-0833.33Transmembrane protein 205 OS=Pongo abelii OX=9601 GN=TMEM205 PE=2 SV=1[more]
Q91XE84.5e-0835.00Transmembrane protein 205 OS=Mus musculus OX=10090 GN=Tmem205 PE=1 SV=1[more]
Q32L101.3e-0734.17Transmembrane protein 205 OS=Bos taurus OX=9913 GN=TMEM205 PE=2 SV=1[more]
Q28GF87.2e-0626.98Transmembrane protein 205 OS=Xenopus tropicalis OX=8364 GN=tmem205 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FL321.3e-24799.56uncharacterized protein LOC111444965 OS=Cucurbita moschata OX=3662 GN=LOC1114449... [more]
A0A6J1JR643.9e-24196.94uncharacterized protein LOC111489083 OS=Cucurbita maxima OX=3661 GN=LOC111489083... [more]
A0A6J1DI199.3e-15069.58uncharacterized protein LOC111020246 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A1S3CA562.4e-14566.09uncharacterized protein LOC103498695 OS=Cucumis melo OX=3656 GN=LOC103498695 PE=... [more]
A0A5D3BMZ02.0e-14465.94DUF4149 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT1G72100.12.0e-6738.28late embryogenesis abundant domain-containing protein / LEA domain-containing pr... [more]
AT1G22600.17.3e-4631.98Late embryogenesis abundant protein (LEA) family protein [more]
AT3G62580.11.1e-0927.60Late embryogenesis abundant protein (LEA) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 183..203
NoneNo IPR availableGENE3D1.20.120.20Apolipoproteincoord: 92..236
e-value: 4.6E-5
score: 25.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..86
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 140..160
NoneNo IPR availablePANTHERPTHR47652FAMILY NOT NAMEDcoord: 1..456
NoneNo IPR availablePANTHERPTHR47652:SF3LATE EMBRYOGENESIS ABUNDANT PROTEIN (LEA) FAMILY PROTEINcoord: 1..456
IPR025423Domain of unknown function DUF4149PFAMPF13664DUF4149coord: 269..371
e-value: 9.2E-18
score: 64.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27297-RACarg27297-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane