Carg26894 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26894
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Mitotic-spindle organizing protein 1
Location	Carg_Chr05: 5503027 .. 5503382 (+)
RNA-Seq Expression	Carg26894
Synteny	Carg26894

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCAGAGGCTGCACGAACGGCTCGGGAATCGCTAGACCTTGCATTTCATATGTGTAACGTAATCGATGCCGGGATCGATCGCCACACCCTCTCTGTCCTTATTGCACTCTGTGACATGGGTGTGAACCCTGAAGCACTGGCTGCTGTTGTCAAGGAACTAAGCAGAGAACCGCCCTCATCGGAAACCATCATCTCAGGTAACAAGTCATAACCTATATGCAAAGAAACAACTCTGTTCTTCTTGTGATGATGGTTGCTAAAGTTGATATCTATGTGTTTTTAAACCACTTTACTATATATGTAGCTTACTTGTTTACTATATATGTAGCTTACTTGGATCTTGTGAGATGA

mRNA sequence

ATGGATCCAGAGGCTGCACGAACGGCTCGGGAATCGCTAGACCTTGCATTTCATATGTGTAACGTAATCGATGCCGGGATCGATCGCCACACCCTCTCTGTCCTTATTGCACTCTGTGACATGGGTGTGAACCCTGAAGCACTGGCTGCTGTTGTCAAGGAACTAAGCAGAGAACCGCCCTCATCGGAAACCATCATCTCAGCTTACTTGGATCTTGTGAGATGA

Coding sequence (CDS)

ATGGATCCAGAGGCTGCACGAACGGCTCGGGAATCGCTAGACCTTGCATTTCATATGTGTAACGTAATCGATGCCGGGATCGATCGCCACACCCTCTCTGTCCTTATTGCACTCTGTGACATGGGTGTGAACCCTGAAGCACTGGCTGCTGTTGTCAAGGAACTAAGCAGAGAACCGCCCTCATCGGAAACCATCATCTCAGCTTACTTGGATCTTGTGAGATGA

Protein sequence

MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPPSSETIISAYLDLVR

Homology

[Close]

BLAST of Carg26894 vs. NCBI nr
Match: KAG7029927.1 (Mitotic-spindle organizing protein 1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 146.7 bits (369), Expect = 7.7e-32
Identity = 74/74 (100.00%), Postives = 74/74 (100.00%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP
Sbjct: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60

Query: 61 SSETIISAYLDLVR 75
          SSETIISAYLDLVR
Sbjct: 61 SSETIISAYLDLVR 74

[Close]

BLAST of Carg26894 vs. NCBI nr
Match: KAG6598967.1 (ALBINO3-like protein 3, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 132.5 bits (332), Expect = 1.5e-27
Identity = 66/67 (98.51%), Postives = 67/67 (100.00%), Query Frame = 0


Query: 1   MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
           MDPEAARTARESLDLAFHMCNV+DAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP
Sbjct: 163 MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 222

Query: 61  SSETIIS 68
           SSETIIS
Sbjct: 223 SSETIIS 229

[Close]

BLAST of Carg26894 vs. NCBI nr
Match: XP_022973921.1 (mitotic-spindle organizing protein 1B-like [Cucurbita maxima] >XP_022973922.1 mitotic-spindle organizing protein 1B-like [Cucurbita maxima])

HSP 1 Score: 126.7 bits (317), Expect = 8.2e-26
Identity = 64/67 (95.52%), Postives = 65/67 (97.01%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MDPEAARTARESLDLAFHM NV+DAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP
Sbjct: 1  MDPEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60

Query: 61 SSETIIS 68
          SSETI S
Sbjct: 61 SSETITS 67

[Close]

BLAST of Carg26894 vs. NCBI nr
Match: XP_038889535.1 (mitotic-spindle organizing protein 1B [Benincasa hispida] >XP_038889536.1 mitotic-spindle organizing protein 1B [Benincasa hispida])

HSP 1 Score: 126.3 bits (316), Expect = 1.1e-25
Identity = 62/68 (91.18%), Postives = 66/68 (97.06%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MDPEAARTARESLDLAFHM NV+DAGIDRHTLSVLIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDPEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSETIISA 69
          SSET++S+
Sbjct: 61 SSETVVSS 68

[Close]

BLAST of Carg26894 vs. NCBI nr
Match: KAE8651738.1 (hypothetical protein Csa_006450 [Cucumis sativus])

HSP 1 Score: 125.6 bits (314), Expect = 1.8e-25
Identity = 63/74 (85.14%), Postives = 67/74 (90.54%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MD EAARTARESLDLAFHM NV+DAGIDRHTLSVLIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSETIISAYLDLVR 75
          SS+TII+   D V+
Sbjct: 61 SSDTIIACLADFVK 74

[Close]

BLAST of Carg26894 vs. ExPASy Swiss-Prot
Match: Q9M0N8 (Mitotic-spindle organizing protein 1B OS=Arabidopsis thaliana OX=3702 GN=GIP1 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 5.9e-19
Identity = 48/65 (73.85%), Postives = 54/65 (83.08%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREP- 60
          MD EA+RTARESL+L F M N++D G+DRHTLSVLIALCD+GVNPEALA VVKEL RE  
Sbjct: 1  MDEEASRTARESLELVFRMSNILDTGLDRHTLSVLIALCDLGVNPEALATVVKELRRESI 60

Query: 61 PSSET 65
          P S T
Sbjct: 61 PDSVT 65

[Close]

BLAST of Carg26894 vs. ExPASy Swiss-Prot
Match: Q9C9T3 (Mitotic-spindle organizing protein 1A OS=Arabidopsis thaliana OX=3702 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.3e-17
Identity = 41/66 (62.12%), Postives = 54/66 (81.82%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          M+ EAA TARESL+L F M N+++ G+DRHTLSVLIALCD+G+NPEALA +VKEL R+  
Sbjct: 1  MNQEAAETARESLELVFRMSNILETGLDRHTLSVLIALCDIGLNPEALATLVKELRRDSA 60

Query: 61 SSETII 67
          ++ T +
Sbjct: 61 TTTTTV 66

[Close]

BLAST of Carg26894 vs. ExPASy Swiss-Prot
Match: A9NKD9 (Mitotic-spindle organizing protein 1 OS=Picea sitchensis OX=3332 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 4.7e-16
Identity = 42/58 (72.41%), Postives = 48/58 (82.76%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSRE 59
          MD  AA+ ARESLDLAF + N +  G+DRHTLS+LIALC+ GVNPEALAAVVKEL RE
Sbjct: 1  MDRTAAQNARESLDLAFSISNFLQTGLDRHTLSILIALCEHGVNPEALAAVVKELRRE 58

[Close]

BLAST of Carg26894 vs. ExPASy Swiss-Prot
Match: B5FXZ4 (Mitotic-spindle organizing protein 1 OS=Taeniopygia guttata OX=59729 GN=mzt1 PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.8e-07
Identity = 21/48 (43.75%), Postives = 37/48 (77.08%), Query Frame = 0


Query: 10 RESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSR 58
          RE++D+ F +  +++ G+D  TLS+ + LC+ G+NPEAL++V+KEL +
Sbjct: 12 RETMDVLFEISRILNTGLDMETLSICVRLCEQGINPEALSSVIKELRK 59

[Close]

BLAST of Carg26894 vs. ExPASy Swiss-Prot
Match: Q0VFD6 (Mitotic-spindle organizing protein 1 OS=Xenopus tropicalis OX=8364 GN=mzt1 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 3.1e-07
Identity = 24/56 (42.86%), Postives = 41/56 (73.21%), Query Frame = 0


Query: 10 RESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPPSSETI 66
          RE++D+   +  +++ G+D  TLS+ + LC+ G+NPEAL++V+KEL R   +SET+
Sbjct: 12 RETMDVLLEISRLLNTGLDMETLSICVRLCEQGINPEALSSVIKELRR---ASETL 64

[Close]

BLAST of Carg26894 vs. ExPASy TrEMBL
Match: A0A6J1I8U7 (mitotic-spindle organizing protein 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111472550 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.0e-26
Identity = 64/67 (95.52%), Postives = 65/67 (97.01%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MDPEAARTARESLDLAFHM NV+DAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP
Sbjct: 1  MDPEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60

Query: 61 SSETIIS 68
          SSETI S
Sbjct: 61 SSETITS 67

[Close]

BLAST of Carg26894 vs. ExPASy TrEMBL
Match: A0A5D3D1N9 (Mitotic-spindle organizing protein 1A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003990 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.5e-25
Identity = 62/68 (91.18%), Postives = 65/68 (95.59%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MD EAARTARESLDLAFHM NV+DAGIDRHTLSVLIALCDMGVNPEALAAVVKEL +EPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRQEPP 60

Query: 61 SSETIISA 69
          +SETIISA
Sbjct: 61 ASETIISA 68

[Close]

BLAST of Carg26894 vs. ExPASy TrEMBL
Match: A0A0A0LMZ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G094370 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.5e-25
Identity = 61/68 (89.71%), Postives = 65/68 (95.59%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MD EAARTARESLDLAFHM NV+DAGIDRHTLSVLIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSETIISA 69
          SS+TI+S+
Sbjct: 61 SSDTIVSS 68

[Close]

BLAST of Carg26894 vs. ExPASy TrEMBL
Match: A0A6J1DE95 (mitotic-spindle organizing protein 1B OS=Momordica charantia OX=3673 GN=LOC111019635 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.2e-24
Identity = 59/67 (88.06%), Postives = 63/67 (94.03%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MDPEAARTARESLDLAFHM N+++ GIDRHTLS+LIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDPEAARTARESLDLAFHMSNILNTGIDRHTLSILIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSETIIS 68
          S ETIIS
Sbjct: 61 SGETIIS 67

[Close]

BLAST of Carg26894 vs. ExPASy TrEMBL
Match: A0A1S3B468 (mitotic-spindle organizing protein 1A-like OS=Cucumis melo OX=3656 GN=LOC103485818 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.9e-24
Identity = 60/68 (88.24%), Postives = 64/68 (94.12%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MD EAARTARESLDLAFHM NV+D+GIDRHTLSVLIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDSGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSETIISA 69
          SSET +S+
Sbjct: 61 SSETNVSS 68

[Close]

BLAST of Carg26894 vs. TAIR 10
Match: AT4G09550.1 (AtGCP3 interacting protein 1 )

HSP 1 Score: 94.4 bits (233), Expect = 4.2e-20
Identity = 48/65 (73.85%), Postives = 54/65 (83.08%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREP- 60
          MD EA+RTARESL+L F M N++D G+DRHTLSVLIALCD+GVNPEALA VVKEL RE  
Sbjct: 1  MDEEASRTARESLELVFRMSNILDTGLDRHTLSVLIALCDLGVNPEALATVVKELRRESI 60

Query: 61 PSSET 65
          P S T
Sbjct: 61 PDSVT 65

[Close]

BLAST of Carg26894 vs. TAIR 10
Match: AT1G73790.1 (Protein of unknown function (DUF3743) )

HSP 1 Score: 88.2 bits (217), Expect = 3.0e-18
Identity = 41/66 (62.12%), Postives = 54/66 (81.82%), Query Frame = 0


Query: 1  MDPEAARTARESLDLAFHMCNVIDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          M+ EAA TARESL+L F M N+++ G+DRHTLSVLIALCD+G+NPEALA +VKEL R+  
Sbjct: 1  MNQEAAETARESLELVFRMSNILETGLDRHTLSVLIALCDIGLNPEALATLVKELRRDSA 60

Query: 61 SSETII 67
          ++ T +
Sbjct: 61 TTTTTV 66

The following BLAST results are available for this feature:

BLAST of Carg26894 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029927.1	7.7e-32	100.00	Mitotic-spindle organizing protein 1B, partial [Cucurbita argyrosperma subsp. ar...	[more]
KAG6598967.1	1.5e-27	98.51	ALBINO3-like protein 3, mitochondrial, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022973921.1	8.2e-26	95.52	mitotic-spindle organizing protein 1B-like [Cucurbita maxima] >XP_022973922.1 mi...	[more]
XP_038889535.1	1.1e-25	91.18	mitotic-spindle organizing protein 1B [Benincasa hispida] >XP_038889536.1 mitoti...	[more]
KAE8651738.1	1.8e-25	85.14	hypothetical protein Csa_006450 [Cucumis sativus]	[more]

BLAST of Carg26894 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M0N8	5.9e-19	73.85	Mitotic-spindle organizing protein 1B OS=Arabidopsis thaliana OX=3702 GN=GIP1 PE...	[more]
Q9C9T3	4.3e-17	62.12	Mitotic-spindle organizing protein 1A OS=Arabidopsis thaliana OX=3702 GN=GIP2 PE...	[more]
A9NKD9	4.7e-16	72.41	Mitotic-spindle organizing protein 1 OS=Picea sitchensis OX=3332 PE=3 SV=1	[more]
B5FXZ4	1.8e-07	43.75	Mitotic-spindle organizing protein 1 OS=Taeniopygia guttata OX=59729 GN=mzt1 PE=...	[more]
Q0VFD6	3.1e-07	42.86	Mitotic-spindle organizing protein 1 OS=Xenopus tropicalis OX=8364 GN=mzt1 PE=3 ...	[more]

BLAST of Carg26894 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I8U7	4.0e-26	95.52	mitotic-spindle organizing protein 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A5D3D1N9	7.5e-25	91.18	Mitotic-spindle organizing protein 1A-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A0A0LMZ6	7.5e-25	89.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G094370 PE=3 SV=1	[more]
A0A6J1DE95	2.2e-24	88.06	mitotic-spindle organizing protein 1B OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A1S3B468	4.9e-24	88.24	mitotic-spindle organizing protein 1A-like OS=Cucumis melo OX=3656 GN=LOC1034858...	[more]

BLAST of Carg26894 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G09550.1	4.2e-20	73.85	AtGCP3 interacting protein 1	[more]
AT1G73790.1	3.0e-18	62.12	Protein of unknown function (DUF3743)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022214	Mitotic-spindle organizing protein 1	PFAM	PF12554	MOZART1	coord: 10..55 e-value: 1.9E-17 score: 62.7
IPR022214	Mitotic-spindle organizing protein 1	PANTHER	PTHR28520	MITOTIC-SPINDLE ORGANIZING PROTEIN 1	coord: 1..63
None	No IPR available	PANTHER	PTHR28520:SF10	MITOTIC-SPINDLE ORGANIZING PROTEIN 1A-LIKE	coord: 1..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26894-RA	Carg26894-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0033566	gamma-tubulin complex localization
biological_process	GO:0051415	microtubule nucleation by interphase microtubule organizing center
biological_process	GO:0090307	mitotic spindle assembly
cellular_component	GO:0000931	gamma-tubulin large complex
cellular_component	GO:0031021	interphase microtubule organizing center
cellular_component	GO:0005819	spindle