Carg26570 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26570
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationCarg_Chr01: 6956660 .. 6960988 (-)
RNA-Seq ExpressionCarg26570
SyntenyCarg26570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATATCGCGTCTGGTCGGGTCCCATCGCTCTCTCTCCATCGTCTTTCCAACTTGATGATCTTCATAATTAGGTTCAGTTTCTTCATCTCAGACGCCAGTTCTTTTCCAATTCTCAACTCGATCACAGCTTCCACACTTCTCGATTCTTTAATTCGGTTTCTTGTCGTTCTTCAATTACGTGCTTAAAAGCGTTTTCGTTTTGATCTTCATTCGATCTTCATTTCCATGTCGATGCTCAACTCCTTCTTCAACAGGGGCTTCAAGGCTGCCCAATGGTATCTCTTCTTTTTATTCTGGTTTCTTTTTTTCCTTTTCCATTTTCGCTCTCATCGTTCTTTGTTAATTCTGTCGGTGACTGCATTCATCAATCGATATGCGATTACAGAATTTTGTTTTTTTTTTTTAATTCTAATTTTCTTCTCTGTATACAGTAAAACCCTGCTCAAATTGACAATTCCACGAATTAAGTTGCTGAGGAATCGGAGGGAGATTCAACTTAAGCAGATGCGCCGAGACATAGCTAAGCTTCTTGAGACGGGTCAAGAAGCTACGGCTCGCATTCGGGTATGTGTTACTTTCTGAAGTGCATTTGATCGGGAGGATCAGAAACAAGGGAATTGTTTGATGTCCTGGAACTTTTTGTTGTCTTAATTAGATCTGCTTTTGCTGCATTCAGGTAGAGCATATAATCAGAGAAGAGAATATGATGGCTGCTCAGGAAATTCTTGAGTTGTTTTGTGAGCTTATTGTTGTTCGTCTCCCAATTATTGAAACACAAAGGTAAACTTTCCGAACGGATTTAATCTTCAAGTTTTTGTGAGGTTTTTAAGTGTTTTCTTGTGGTTATGTAAATAAGCACGAGAAAGATCTGAATGCAACTGATTTAAATAGATTACAGTGTTTTCATGGACCCTGTAATCTATGTTTGGCAATTATGAAACACATTTTATTCTAGGAAACATTTATTTATCCAATATGCACATAACTGACAAAACTAGACTAGGATGTTAGATCTCTTAAGCGGTTCTAAAATTTAAATCAATTCTGGACTTCTCTACAAGTATAGTTGTATAAAGGATTTCTATCTTTTTAGGAGCATTTAATTTCTATGATTAGATATGTTTATATTTGGTTAGAGATTTCCTTCATAAAGTGTGACTGATAATTGAGAAGCTGCATCATATATAAATATGTAATTGATGTCAAAGTTTCTCACAGATGTTGTATCCACACTATGGTCCATTCTTTCCCATTGTTGAATGAAGTGAATTTGTCCTAATGTTTGGCCTCACTCCTCGTCTATTAGTAAATTGCTTGTGGCTCTCTCTTTCTTGGGTTACTGTCGTGTTTGTATAACCCATCGTTAGTATACATCTATGTTCTCTTTTGGTTGATAAGAGAGAACATTAGAAATATGCTGGTTAAGTTTGAATGGATATATGCACAAAATGAATTTTAGTGAATTATGTGAAGAATATTCTTTCACCCTAGTTTGGAGTATTGAAGATCCATCTCACAATGATGGAAATTAAGTCATAGATTGTGCATTGTTAACAAGAACTACAGCTAGTTGTCCAACCTGATCATCCAACTTCTCTGCCAAATGACTCGAAGCATCAAGCCTTGCAAACTGGAAATATAGTAGCAGTTGATTTTACTTCGAGGTTGATTGTTAAATTTACAAGGAACTGAATTAGTTTGCACTTTTAAATTTACAATTTTTATGGTTTAATCACCATGCTCTCCTTTTTTTTTGTTGAAAATCTTCGTCATTTTTTGGATAATAGCTTTTGTCCAAAGTTACTTGAAACTCAAAACTTCATGTCCAAATTTGTATGACTGTAAAATCTTTTGATCTTATTCTCTCTTTATTTCCAGGTTTATTTTTGTTTTTATTTTTATTTGTATTTGTGAGTGTTCAAGCTGGATTGGTGCACCTCGACTAATCTCACTGGTCAACCACTTGACTCTATTAGATTTGGTTGACAAAAAAAAAAACTTGTATGATATTAAATTGTATCTTTCTATGCTTCTATATGAAGTACAAATGAACCCTTTCATTCTAATTTCACTTTGTTGCTTATGGTAGTTGTATTGAACATTTAGCCCTTCTATTTTATGCTTGTATCGAATGTCATTGTAAACTCATGAAATTTTCAGGGAATGTCCTCTAGACTTGAAAGAAGCAATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGTGCAGATCTAACAGAGCTTCTCCAGGTTCAGTTGCTTTTTGCAGCTAAATATGGGAAGGAATTTGTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGTGTTAACCGTCAGGTTCATAGAATCTTGTTTATGTTAGATTTATTGGATGAAAAGCTTTACTGCAGTCGATAGGATTTTCATATTTCCTCTCAATTTTGATTGCAGTTGATCGAGTTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTCTTAAAGGAAATCGCTGAAGAACATGACTTAGATTGGGATCCTGCTGGAACTGAAGCTGAGTTTAACAAATCTCCAGAAGATTTGCTCGTAAGTGTTGTTCCTTTTTGTCTCAACTTGCGGTGATTCTTTGTGTCTTAGTTTCTGTTCTCTGTCCTTGCAGAATGGATCAGCGCAGTTTGTCAGTGCGTCGAAATTAGCCCTTCCTAAAGATAAACACGACGAGACATATAGTGCTACTCCTGATTTGGGTAGTGCTCCACAACCTGATTCTGATTCTGAATTGGACACGTTAGACTTTCCTGAAGTTCCAAAGATATCAGTATCTCTGCATCCTCCTACTACTTTTGATGCTGCCACTGCACAACCACTGATTCCACCTCCATCTGTATCCCCACCACCTGAAACTGATCGTGATTCATTCAAATCTTCTGGTATTATTCCTGAAATCCCACCTCAAGATTTACACTTGAAACGTGAGGAGGTGAGATCTGTTTCACCCAGCAACGATCATCTGAATGTCTCTGTTGGCGAAGATAAACAGTTTTTGCCATTTATTACTCCGCCATCACTGTCATCTTCGTTTTCTCATAGACAAACTGATGTGTGCCCATCCTCTGATTCAAAGACCCCTGAGGAGAAGTTTGGTTTCAAACCACGGTTTGAACAAGAGATAAATTCACCACCACCCTCTGTTTCAAGGACCCATGAGGAGACAAATTCACTATCACCTTCTGTTTCAAGGACCCATGAGGAGACAAATTCACTACGTCCCTCTGTTTCTCCTGAAGAAAAATTCGGTTTCAAACCACAGTTTGAGCAGAATCTAGATTCCCCACCACCCTCTGTTTCAAGGACAAAGAGTGAGGTCAATGTGGATGTCAGTGTGGATTTGCAGGATGTGTTGGCAGCTGCTCAGGCTGCTGCTGAAACTGCTGAACGTGCTGCTGCGGCTGCTCGCTCTGCAGCCAGTCTTGCGAAGGTTAGAATCGATGAGCTCACAAAGAAAAAGAATGATCGAGATGCTGAAGTTAGTTGTGAGAATCCATTCCACGGAGCTGCTGTAACTCCACCTGATAAACAAACATTCCAACAAGATTCTTTGGGGAATTATTCTATGCCATATTATTCACAAGATTCTTTCAAAGAACATCACAGGGTAAGCTGTGATTTAGACGTTACTGGTCATCACCAGCTTGAGCCACAGAAATCGGGTTTTGATTCCTCGCCCAGTAATTCACCATCAGAACGCATAGCACATCCTCATCAGACGCAGAGGCTCCCATCGATGGATGACGACCCATACTTTTCATACCCGAATCTATTTACTTCACAGAAACCAAATCTGGGATCTGATCGTTCGTCTGGTGGTGGTTCCGATCTACACATGTGAGGTATGAGTGGAATTTTATATGACATGCTTTCGTGAATTTATATTTTGTTCAGCTTTCATAACGAGTTTCAAATATCCTTTTAGAGCTGTGGTAGGGAGCATTGTATTGTACACACCCGAAAAAGTATATGTTTACACCATTAGAATTAGTTTGTGTTAAGGGGGTAATATGCATTTTTTAAGTGCCTTCAATTTGTATTTATTTGGATATTTCAGAATTCACTTTTATAATAATGGAATCAGAATAGTGTGTTTTAGGATCCAAAGATTGAAGAAAATTGTGATGGGTGAAGGGTACGATCAAGGATACGATCAAGGATTACTCTATCAACCAAAGGGATCGTCGGAGTTTTGAAATGAATGGAAAGACATTCGGATTACACCTATTATCTCAAGTTTTAGATCGGTCCTCTCGTCCTAGGGAAAGATCCTCTCAATGCTCTAACGCAGAACAATTAG

mRNA sequence

AAATATCGCGTCTGGTCGGGTCCCATCGCTCTCTCTCCATCGTCTTTCCAACTTGATGATCTTCATAATTAGGTTCAGTTTCTTCATCTCAGACGCCAGTTCTTTTCCAATTCTCAACTCGATCACAGCTTCCACACTTCTCGATTCTTTAATTCGGTTTCTTGTCGTTCTTCAATTACGTGCTTAAAAGCGTTTTCGTTTTGATCTTCATTCGATCTTCATTTCCATGTCGATGCTCAACTCCTTCTTCAACAGGGGCTTCAAGGCTGCCCAATGTAAAACCCTGCTCAAATTGACAATTCCACGAATTAAGTTGCTGAGGAATCGGAGGGAGATTCAACTTAAGCAGATGCGCCGAGACATAGCTAAGCTTCTTGAGACGGGTCAAGAAGCTACGGCTCGCATTCGGGTAGAGCATATAATCAGAGAAGAGAATATGATGGCTGCTCAGGAAATTCTTGAGTTGTTTTGTGAGCTTATTGTTGTTCGTCTCCCAATTATTGAAACACAAAGGGAATGTCCTCTAGACTTGAAAGAAGCAATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGTGCAGATCTAACAGAGCTTCTCCAGGTTCAGTTGCTTTTTGCAGCTAAATATGGGAAGGAATTTGTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGTGTTAACCGTCAGTTGATCGAGTTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTCTTAAAGGAAATCGCTGAAGAACATGACTTAGATTGGGATCCTGCTGGAACTGAAGCTGAGTTTAACAAATCTCCAGAAGATTTGCTCAATGGATCAGCGCAGTTTGTCAGTGCGTCGAAATTAGCCCTTCCTAAAGATAAACACGACGAGACATATAGTGCTACTCCTGATTTGGGTAGTGCTCCACAACCTGATTCTGATTCTGAATTGGACACGTTAGACTTTCCTGAAGTTCCAAAGATATCAGTATCTCTGCATCCTCCTACTACTTTTGATGCTGCCACTGCACAACCACTGATTCCACCTCCATCTGTATCCCCACCACCTGAAACTGATCGTGATTCATTCAAATCTTCTGGTATTATTCCTGAAATCCCACCTCAAGATTTACACTTGAAACGTGAGGAGGTGAGATCTGTTTCACCCAGCAACGATCATCTGAATGTCTCTGTTGGCGAAGATAAACAGTTTTTGCCATTTATTACTCCGCCATCACTGTCATCTTCGTTTTCTCATAGACAAACTGATGTGTGCCCATCCTCTGATTCAAAGACCCCTGAGGAGAAGTTTGGTTTCAAACCACGGTTTGAACAAGAGATAAATTCACCACCACCCTCTGTTTCAAGGACCCATGAGGAGACAAATTCACTATCACCTTCTGTTTCAAGGACCCATGAGGAGACAAATTCACTACGTCCCTCTGTTTCTCCTGAAGAAAAATTCGGTTTCAAACCACAGTTTGAGCAGAATCTAGATTCCCCACCACCCTCTGTTTCAAGGACAAAGAGTGAGGTCAATGTGGATGTCAGTGTGGATTTGCAGGATGTGTTGGCAGCTGCTCAGGCTGCTGCTGAAACTGCTGAACGTGCTGCTGCGGCTGCTCGCTCTGCAGCCAGTCTTGCGAAGGTTAGAATCGATGAGCTCACAAAGAAAAAGAATGATCGAGATGCTGAAGTTAGTTGTGAGAATCCATTCCACGGAGCTGCTGTAACTCCACCTGATAAACAAACATTCCAACAAGATTCTTTGGGGAATTATTCTATGCCATATTATTCACAAGATTCTTTCAAAGAACATCACAGGGTAAGCTGTGATTTAGACGTTACTGGTCATCACCAGCTTGAGCCACAGAAATCGGGTTTTGATTCCTCGCCCAGTAATTCACCATCAGAACGCATAGCACATCCTCATCAGACGCAGAGGCTCCCATCGATGGATGACGACCCATACTTTTCATACCCGAATCTATTTACTTCACAGAAACCAAATCTGGGATCTGATCGTTCGTCTGGTGGTGGTTCCGATCTACACATGTGAGGTATGAGTGGAATTTTATATGACATGCTTTCGTGAATTTATATTTTGTTCAGCTTTCATAACGAGTTTCAAATATCCTTTTAGAGCTGTGGTAGGGAGCATTGTATTGTACACACCCGAAAAAGTATATGTTTACACCATTAGAATTAGTTTGTGTTAAGGGGGTAATATGCATTTTTTAAGTGCCTTCAATTTGTATTTATTTGGATATTTCAGAATTCACTTTTATAATAATGGAATCAGAATAGTGTGTTTTAGGATCCAAAGATTGAAGAAAATTGTGATGGGTGAAGGGTACGATCAAGGATACGATCAAGGATTACTCTATCAACCAAAGGGATCGTCGGAGTTTTGAAATGAATGGAAAGACATTCGGATTACACCTATTATCTCAAGTTTTAGATCGGTCCTCTCGTCCTAGGGAAAGATCCTCTCAATGCTCTAACGCAGAACAATTAG

Coding sequence (CDS)

ATGTCGATGCTCAACTCCTTCTTCAACAGGGGCTTCAAGGCTGCCCAATGTAAAACCCTGCTCAAATTGACAATTCCACGAATTAAGTTGCTGAGGAATCGGAGGGAGATTCAACTTAAGCAGATGCGCCGAGACATAGCTAAGCTTCTTGAGACGGGTCAAGAAGCTACGGCTCGCATTCGGGTAGAGCATATAATCAGAGAAGAGAATATGATGGCTGCTCAGGAAATTCTTGAGTTGTTTTGTGAGCTTATTGTTGTTCGTCTCCCAATTATTGAAACACAAAGGGAATGTCCTCTAGACTTGAAAGAAGCAATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGTGCAGATCTAACAGAGCTTCTCCAGGTTCAGTTGCTTTTTGCAGCTAAATATGGGAAGGAATTTGTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGTGTTAACCGTCAGTTGATCGAGTTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTCTTAAAGGAAATCGCTGAAGAACATGACTTAGATTGGGATCCTGCTGGAACTGAAGCTGAGTTTAACAAATCTCCAGAAGATTTGCTCAATGGATCAGCGCAGTTTGTCAGTGCGTCGAAATTAGCCCTTCCTAAAGATAAACACGACGAGACATATAGTGCTACTCCTGATTTGGGTAGTGCTCCACAACCTGATTCTGATTCTGAATTGGACACGTTAGACTTTCCTGAAGTTCCAAAGATATCAGTATCTCTGCATCCTCCTACTACTTTTGATGCTGCCACTGCACAACCACTGATTCCACCTCCATCTGTATCCCCACCACCTGAAACTGATCGTGATTCATTCAAATCTTCTGGTATTATTCCTGAAATCCCACCTCAAGATTTACACTTGAAACGTGAGGAGGTGAGATCTGTTTCACCCAGCAACGATCATCTGAATGTCTCTGTTGGCGAAGATAAACAGTTTTTGCCATTTATTACTCCGCCATCACTGTCATCTTCGTTTTCTCATAGACAAACTGATGTGTGCCCATCCTCTGATTCAAAGACCCCTGAGGAGAAGTTTGGTTTCAAACCACGGTTTGAACAAGAGATAAATTCACCACCACCCTCTGTTTCAAGGACCCATGAGGAGACAAATTCACTATCACCTTCTGTTTCAAGGACCCATGAGGAGACAAATTCACTACGTCCCTCTGTTTCTCCTGAAGAAAAATTCGGTTTCAAACCACAGTTTGAGCAGAATCTAGATTCCCCACCACCCTCTGTTTCAAGGACAAAGAGTGAGGTCAATGTGGATGTCAGTGTGGATTTGCAGGATGTGTTGGCAGCTGCTCAGGCTGCTGCTGAAACTGCTGAACGTGCTGCTGCGGCTGCTCGCTCTGCAGCCAGTCTTGCGAAGGTTAGAATCGATGAGCTCACAAAGAAAAAGAATGATCGAGATGCTGAAGTTAGTTGTGAGAATCCATTCCACGGAGCTGCTGTAACTCCACCTGATAAACAAACATTCCAACAAGATTCTTTGGGGAATTATTCTATGCCATATTATTCACAAGATTCTTTCAAAGAACATCACAGGGTAAGCTGTGATTTAGACGTTACTGGTCATCACCAGCTTGAGCCACAGAAATCGGGTTTTGATTCCTCGCCCAGTAATTCACCATCAGAACGCATAGCACATCCTCATCAGACGCAGAGGCTCCCATCGATGGATGACGACCCATACTTTTCATACCCGAATCTATTTACTTCACAGAAACCAAATCTGGGATCTGATCGTTCGTCTGGTGGTGGTTCCGATCTACACATGTGA

Protein sequence

MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSELDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQDLHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEKFGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQNLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDRDAEVSCENPFHGAAVTPPDKQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDVTGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDRSSGGGSDLHM
Homology
BLAST of Carg26570 vs. NCBI nr
Match: KAG7037225.1 (IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 609/609 (100.00%), Postives = 609/609 (100.00%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD
Sbjct: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300

Query: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360
           LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK
Sbjct: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360

Query: 361 FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420
           FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ
Sbjct: 361 FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420

Query: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480
           NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT
Sbjct: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480

Query: 481 KKKNDRDAEVSCENPFHGAAVTPPDKQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDVT 540
           KKKNDRDAEVSCENPFHGAAVTPPDKQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDVT
Sbjct: 481 KKKNDRDAEVSCENPFHGAAVTPPDKQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDVT 540

Query: 541 GHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDRS 600
           GHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDRS
Sbjct: 541 GHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDRS 600

Query: 601 SGGGSDLHM 610
           SGGGSDLHM
Sbjct: 601 SGGGSDLHM 609

BLAST of Carg26570 vs. NCBI nr
Match: XP_022926259.1 (uncharacterized protein LOC111433438 [Cucurbita moschata])

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 605/610 (99.18%), Postives = 606/610 (99.34%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKL LPKDKHDETYSATPDLGSAPQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LD LDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD
Sbjct: 241 LDPLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300

Query: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360
           LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK
Sbjct: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360

Query: 361 FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420
           FGFKPRFEQEINSPPPSVSRTHEE NSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ
Sbjct: 361 FGFKPRFEQEINSPPPSVSRTHEERNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420

Query: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480
           NLDSPPPSVSRTKSEVNVDVSV+LQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT
Sbjct: 421 NLDSPPPSVSRTKSEVNVDVSVNLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480

Query: 481 KKKNDRDAEVSCENPFHGAAVTPPD-KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV 540
           KKKNDRDAEVSCENPFHGAAVTPPD KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV
Sbjct: 481 KKKNDRDAEVSCENPFHGAAVTPPDHKQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV 540

Query: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600
           TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR
Sbjct: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600

Query: 601 SSGGGSDLHM 610
           SSGGGSDLHM
Sbjct: 601 SSGGGSDLHM 610

BLAST of Carg26570 vs. NCBI nr
Match: XP_023521005.1 (uncharacterized protein LOC111784587 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1148.7 bits (2970), Expect = 0.0e+00
Identity = 598/610 (98.03%), Postives = 601/610 (98.52%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKL LPKDKHDETYSATPDLGSAPQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD
Sbjct: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300

Query: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360
           LHL+ EEVRSVSPSNDHLN+SVGEDKQFLPFITPPSLSSSFSHRQTD+CPSSDSKTPEEK
Sbjct: 301 LHLRHEEVRSVSPSNDHLNISVGEDKQFLPFITPPSLSSSFSHRQTDLCPSSDSKTPEEK 360

Query: 361 FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420
            GFKPRFEQEINSPPPSVSRTHEE NSLSPSVSRTHEE NSL PSVSPEEKFGFKPQFEQ
Sbjct: 361 LGFKPRFEQEINSPPPSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKPQFEQ 420

Query: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480
           NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT
Sbjct: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480

Query: 481 KKKNDRDAEVSCENPFHGAAVTPPD-KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV 540
           KKKNDRDAEVSCENPFHGAAVTPPD KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCD DV
Sbjct: 481 KKKNDRDAEVSCENPFHGAAVTPPDHKQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDSDV 540

Query: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600
           TGHHQLEPQKSGFDSSPSNSPSERIAHPHQ QRLPSMDDDPYFSYPNLFTSQKPNLGSDR
Sbjct: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600

Query: 601 SSGGGSDLHM 610
           SSGGGSDLHM
Sbjct: 601 SSGGGSDLHM 610

BLAST of Carg26570 vs. NCBI nr
Match: KAG6607619.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 596/610 (97.70%), Postives = 599/610 (98.20%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKL LPKDKHDETYSATPDLGSAPQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD
Sbjct: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300

Query: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360
           LHL+ EEVRSVSPSNDHLN+SVGEDKQFLPFITPPSLSSS SHRQTD+CPSSDSKTPEEK
Sbjct: 301 LHLRHEEVRSVSPSNDHLNISVGEDKQFLPFITPPSLSSSSSHRQTDLCPSSDSKTPEEK 360

Query: 361 FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420
            GFKPRFEQEINSPPPSVSRTHEE NSLSPSVSRTHEE NSL PSVSPEEKFGFKPQFEQ
Sbjct: 361 LGFKPRFEQEINSPPPSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKPQFEQ 420

Query: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480
           NLDSPPPSVSR KSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT
Sbjct: 421 NLDSPPPSVSRIKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480

Query: 481 KKKNDRDAEVSCENPFHGAAVTPPD-KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV 540
           KKKNDRDAEVSCENPFHGAAVTPPD KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCD DV
Sbjct: 481 KKKNDRDAEVSCENPFHGAAVTPPDHKQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDSDV 540

Query: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600
           TGHHQLEPQKSGFDSSPSNSPSERIAHPHQ QRLPSMDDDPYFSYPNLFTSQKPNLGSDR
Sbjct: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600

Query: 601 SSGGGSDLHM 610
           SSGGGSDLHM
Sbjct: 601 SSGGGSDLHM 610

BLAST of Carg26570 vs. NCBI nr
Match: XP_022981875.1 (uncharacterized protein LOC111480885 [Cucurbita maxima])

HSP 1 Score: 1140.9 bits (2950), Expect = 0.0e+00
Identity = 595/610 (97.54%), Postives = 600/610 (98.36%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFN+GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKL LPKDKHDETYSATPDLGSAPQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETD DSFKSSGIIPEIPPQD
Sbjct: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDCDSFKSSGIIPEIPPQD 300

Query: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360
           LHL+ EEVRSVSPSNDHLN+SVGEDKQFLPFITPPSLSSSFSHRQTD+CPSSDSKTPEEK
Sbjct: 301 LHLRHEEVRSVSPSNDHLNISVGEDKQFLPFITPPSLSSSFSHRQTDLCPSSDSKTPEEK 360

Query: 361 FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420
           FGFKPRFEQEINSPP SVSRTHEE NSLSPSVSRTHEE NSL PSVSPEEKFGFKPQFEQ
Sbjct: 361 FGFKPRFEQEINSPPLSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKPQFEQ 420

Query: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480
           NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT
Sbjct: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480

Query: 481 KKKNDRDAEVSCENPFHGAAVTPPD-KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV 540
           KKKNDRDAEVSCENPFHGAAVTPPD KQTFQQDSLG+YSMPYYSQDSFKEHHRVSCD DV
Sbjct: 481 KKKNDRDAEVSCENPFHGAAVTPPDHKQTFQQDSLGDYSMPYYSQDSFKEHHRVSCDSDV 540

Query: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600
           TGHHQLEPQKSGFDSSPSNSPSERIAHPHQ QRLPSMDDDPYFSYPNLFTSQKPNLGSDR
Sbjct: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600

Query: 601 SSGGGSDLHM 610
           SSGGGSDLHM
Sbjct: 601 SSGGGSDLHM 610

BLAST of Carg26570 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-20
Identity = 66/176 (37.50%), Postives = 107/176 (60.80%), Query Frame = 0

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQLLF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 AAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 186
            AKY KE+            VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Carg26570 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-20
Identity = 66/176 (37.50%), Postives = 107/176 (60.80%), Query Frame = 0

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQLLF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 AAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 186
            AKY KE+            VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Carg26570 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-20
Identity = 66/176 (37.50%), Postives = 107/176 (60.80%), Query Frame = 0

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQLLF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 AAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 186
            AKY KE+            VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Carg26570 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.2e-20
Identity = 65/176 (36.93%), Postives = 106/176 (60.23%), Query Frame = 0

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           G KA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQLLF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 AAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 186
            AKY KE+            VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 125 CAKYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Carg26570 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 6.8e-20
Identity = 65/176 (36.93%), Postives = 106/176 (60.23%), Query Frame = 0

Query: 11  GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEN 70
           GFKA + +  L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ 
Sbjct: 5   GFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 71  MMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQLLF 130
           ++ A EILEL+C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 131 AAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 186
            AKY K +            VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 125 CAKYSKGYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Carg26570 vs. ExPASy TrEMBL
Match: A0A6J1EEM3 (uncharacterized protein LOC111433438 OS=Cucurbita moschata OX=3662 GN=LOC111433438 PE=3 SV=1)

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 605/610 (99.18%), Postives = 606/610 (99.34%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKL LPKDKHDETYSATPDLGSAPQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LD LDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD
Sbjct: 241 LDPLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300

Query: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360
           LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK
Sbjct: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360

Query: 361 FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420
           FGFKPRFEQEINSPPPSVSRTHEE NSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ
Sbjct: 361 FGFKPRFEQEINSPPPSVSRTHEERNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420

Query: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480
           NLDSPPPSVSRTKSEVNVDVSV+LQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT
Sbjct: 421 NLDSPPPSVSRTKSEVNVDVSVNLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480

Query: 481 KKKNDRDAEVSCENPFHGAAVTPPD-KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV 540
           KKKNDRDAEVSCENPFHGAAVTPPD KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV
Sbjct: 481 KKKNDRDAEVSCENPFHGAAVTPPDHKQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV 540

Query: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600
           TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR
Sbjct: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600

Query: 601 SSGGGSDLHM 610
           SSGGGSDLHM
Sbjct: 601 SSGGGSDLHM 610

BLAST of Carg26570 vs. ExPASy TrEMBL
Match: A0A6J1J0W1 (uncharacterized protein LOC111480885 OS=Cucurbita maxima OX=3661 GN=LOC111480885 PE=3 SV=1)

HSP 1 Score: 1140.9 bits (2950), Expect = 0.0e+00
Identity = 595/610 (97.54%), Postives = 600/610 (98.36%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFN+GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKL LPKDKHDETYSATPDLGSAPQPDSDSE
Sbjct: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETD DSFKSSGIIPEIPPQD
Sbjct: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDCDSFKSSGIIPEIPPQD 300

Query: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360
           LHL+ EEVRSVSPSNDHLN+SVGEDKQFLPFITPPSLSSSFSHRQTD+CPSSDSKTPEEK
Sbjct: 301 LHLRHEEVRSVSPSNDHLNISVGEDKQFLPFITPPSLSSSFSHRQTDLCPSSDSKTPEEK 360

Query: 361 FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420
           FGFKPRFEQEINSPP SVSRTHEE NSLSPSVSRTHEE NSL PSVSPEEKFGFKPQFEQ
Sbjct: 361 FGFKPRFEQEINSPPLSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKPQFEQ 420

Query: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480
           NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT
Sbjct: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480

Query: 481 KKKNDRDAEVSCENPFHGAAVTPPD-KQTFQQDSLGNYSMPYYSQDSFKEHHRVSCDLDV 540
           KKKNDRDAEVSCENPFHGAAVTPPD KQTFQQDSLG+YSMPYYSQDSFKEHHRVSCD DV
Sbjct: 481 KKKNDRDAEVSCENPFHGAAVTPPDHKQTFQQDSLGDYSMPYYSQDSFKEHHRVSCDSDV 540

Query: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600
           TGHHQLEPQKSGFDSSPSNSPSERIAHPHQ QRLPSMDDDPYFSYPNLFTSQKPNLGSDR
Sbjct: 541 TGHHQLEPQKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDR 600

Query: 601 SSGGGSDLHM 610
           SSGGGSDLHM
Sbjct: 601 SSGGGSDLHM 610

BLAST of Carg26570 vs. ExPASy TrEMBL
Match: A0A0A0KBZ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G020110 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 3.3e-243
Identity = 471/615 (76.59%), Postives = 510/615 (82.93%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFN+GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL+QVQ+LF AKYGKEF+SAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELIQVQMLFGAKYGKEFLSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDW+PA TEAEFNKSPEDLLNGS QFV ASKL LP++KH+ET + T DL S PQPDSDSE
Sbjct: 181 LDWNPAETEAEFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LD LDFPEVPK+SV   P  T DA +A  +IPPPSVSPP ETD  SF+ SG IPE PPQ+
Sbjct: 241 LDMLDFPEVPKMSVC--PQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSG-IPESPPQN 300

Query: 301 LHLKREE---VRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTP 360
           LH + EE   VRSVSPSND +NVSVGEDKQFLPFITPPSLS SFS RQT++ PSSD  TP
Sbjct: 301 LHSRHEEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTP 360

Query: 361 EEKFGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQ 420
           EEKF  KPR  +EINSP PSVSR                          + EEK+GFKP+
Sbjct: 361 EEKFDIKPRIREEINSPLPSVSR--------------------------ASEEKYGFKPR 420

Query: 421 FEQNLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRID 480
           FE  ++S P SVSRT SEVN D+ VDLQDVLAAAQAAAETAERAAAAARSAASLAKVRID
Sbjct: 421 FEPEINSTPSSVSRTMSEVNADLGVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRID 480

Query: 481 ELTKKKNDRDAEVSCENPFHGAAVTPPD-KQTF---QQDSLGNYS--MPYYSQDSFKEHH 540
           ELTKKKND+D E+SCENPFHG   +P D  +T+   QQDSLG +S  MPYYSQDSF+EHH
Sbjct: 481 ELTKKKNDKDPEISCENPFHGGTASPADHTETYKLNQQDSLGYHSSAMPYYSQDSFQEHH 540

Query: 541 RVSCDLDVTGHHQLEPQKSGFDSSPSNSPSE-RIAHPHQTQRLPSMDDDPYFSYPNLFTS 600
           ++S D +V+ H QL+P+KS FD SP NSPSE +   PHQ QRLPSMDDDPYFSYPNLFTS
Sbjct: 541 KLSRDPEVSDHSQLKPEKSSFDYSPGNSPSEHQTVAPHQPQRLPSMDDDPYFSYPNLFTS 586

Query: 601 QKPNLGSDRSSGGGS 606
           QKPN G D SS GGS
Sbjct: 601 QKPNPGFDHSSAGGS 586

BLAST of Carg26570 vs. ExPASy TrEMBL
Match: A0A1S4E4M8 (uncharacterized protein LOC103502259 OS=Cucumis melo OX=3656 GN=LOC103502259 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 4.4e-240
Identity = 470/615 (76.42%), Postives = 507/615 (82.44%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSMLNSFFN+GFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLNSFFNKGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL+QVQ+LF AKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELIQVQMLFGAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDW+PA TEAEFNKSPEDLLNG   FV  SKL LP++KH+ET + T DL S PQPDSDSE
Sbjct: 181 LDWNPAETEAEFNKSPEDLLNG---FVGTSKLPLPQEKHNETSNTTTDLASGPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LD LDFPEVPK+SV   P  T DA +A  +I PPSVSP PETDR SFK SG IPE PPQ+
Sbjct: 241 LDMLDFPEVPKMSVC--PQPTIDAGSAPSMILPPSVSPQPETDRASFKYSG-IPESPPQN 300

Query: 301 LHLKREE---VRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTP 360
           LH +  E   VRSVSPSND +NVSVGEDKQFLPFITPPSLS SFSHRQTD+ P SD  TP
Sbjct: 301 LHSRHGEVTLVRSVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSHRQTDLSPPSDPTTP 360

Query: 361 EEKFGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQ 420
           EEKF  KPR  +EINSP PSVSR                          + EE+FGFKP+
Sbjct: 361 EEKFDIKPRITEEINSPLPSVSR--------------------------ASEEQFGFKPR 420

Query: 421 FEQNLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRID 480
           F+  ++S P SVSRT  EVN D+SVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRID
Sbjct: 421 FDSEINSTPSSVSRTMCEVNADLSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRID 480

Query: 481 ELTKKKNDRDAEVSCENPFHGAAVTPP-DKQTF---QQDSLGNY--SMPYYSQDSFKEHH 540
           ELTKKKND+D E+SCENPFHGA  +P    +T+   QQDSLG++  SMPYYSQDS +EHH
Sbjct: 481 ELTKKKNDKDPEISCENPFHGATASPAGHTETYKLNQQDSLGHHSSSMPYYSQDSSQEHH 540

Query: 541 RVSCDLDVTGHHQLEPQKSGFDSSPSNSPSE-RIAHPHQTQRLPSMDDDPYFSYPNLFTS 600
           ++S D +V+ H QLEP+KS FDSSP NSPSE +   PHQ QRLPSMDDDPYFSYPNLFTS
Sbjct: 541 KLSRDPEVSDHSQLEPEKSSFDSSPGNSPSEHQTVAPHQPQRLPSMDDDPYFSYPNLFTS 583

Query: 601 QKPNLGSDRSSGGGS 606
           QKPN G D SS GGS
Sbjct: 601 QKPNPGFDHSSAGGS 583

BLAST of Carg26570 vs. ExPASy TrEMBL
Match: A0A6J1EU69 (uncharacterized protein LOC111436655 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436655 PE=3 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.8e-228
Identity = 455/615 (73.98%), Postives = 487/615 (79.19%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML SFF +GFK AQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI
Sbjct: 1   MSMLASFFYKGFKGAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT
Sbjct: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           ELLQVQLLFA KYGKEFVSAATELMPNCGVNRQL+ELLSVRAPSPEKKLKLLKEIAEEHD
Sbjct: 121 ELLQVQLLFAVKYGKEFVSAATELMPNCGVNRQLVELLSVRAPSPEKKLKLLKEIAEEHD 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           +DWDPAGTEAEFNKSPEDLLNGSAQFVSASKL LP++KH+E Y       +APQPDSDSE
Sbjct: 181 VDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAY-------NAPQPDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
           LD LDFPEVPKISVS  P +T D  +A  +IPPPS SPPPET  DS K S  IPE PP+D
Sbjct: 241 LDMLDFPEVPKISVS--PHSTIDPGSASSMIPPPSTSPPPETTHDSSKYS-CIPETPPED 300

Query: 301 LHLKREE---VRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTP 360
           L L+R E   VRSVSPS D +N+SVGEDKQFLPFI PP                  S+TP
Sbjct: 301 LQLRRGEVTPVRSVSPSKDQMNISVGEDKQFLPFIAPP------------------SRTP 360

Query: 361 EEKFGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQ 420
           EEKF FKP+FE EINSPPPSVSRT                          P+EKFG K  
Sbjct: 361 EEKFSFKPQFEPEINSPPPSVSRT--------------------------PDEKFGLK-- 420

Query: 421 FEQNLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRID 480
           +EQ + SPPPS SRTKS    DVS+DLQDVLAAAQAA ETAERAAAAARSAASL KVRID
Sbjct: 421 YEQEIISPPPSASRTKS----DVSLDLQDVLAAAQAAVETAERAAAAARSAASLTKVRID 480

Query: 481 ELTKKKNDRDAEVSCENPFHGAAVTPPD-KQTF---QQDSLGNY---SMPYYSQDSFKEH 540
            LT K ND+  E+ CENPFHGA  +PPD +QTF   QQDS+G++   SMPYYSQDSF+EH
Sbjct: 481 TLTMKTNDQGPEICCENPFHGATTSPPDHEQTFHLNQQDSIGHHHSSSMPYYSQDSFQEH 540

Query: 541 HRVSCDLDVTGHHQLEPQKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTS 600
           H+ S   DV+ HH+ E QKSGFDSSP +SPSE I  PHQ QRLPSMDDDPYFSYPNLFTS
Sbjct: 541 HKESPGSDVSDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRLPSMDDDPYFSYPNLFTS 555

Query: 601 QKPNLGSDRSSGGGS 606
           QKPN G D SS  GS
Sbjct: 601 QKPNPGFDHSSADGS 555

BLAST of Carg26570 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 409.1 bits (1050), Expect = 6.4e-114
Identity = 309/704 (43.89%), Postives = 377/704 (53.55%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML+SFFN+GFKAA+CKTLLKLTIPRIKL+RNRRE Q+KQMRR+IAKLLETGQEATARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREE MMAAQEILELFCELI VRLPIIE QRECPLDLKEAISSVCFAAPRC+DLT
Sbjct: 61  RVEHIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           EL QVQ+LF +KYGKEFV+AA+EL P+ GVNR+L+ELLSVRAPSPE KLKLLKEIAEEH+
Sbjct: 121 ELQQVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEHE 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSE 240
           LDWDPA TE +  KS EDLL+G  QF   SKL LP++++++T + T    +  + DSDSE
Sbjct: 181 LDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKT-NLTSLSAAKEKSDSDSE 240

Query: 241 LDTLDFPEVPKISVSLHPPTTFDAATAQPLIPPPSVSPPPETDRDSFKSSGIIPEIPPQD 300
            D LDFPEVP + +   P  T             SV+ P     D+ K            
Sbjct: 241 YDILDFPEVPNVLLRPTPGAT-------------SVNAP-----DAAK------------ 300

Query: 301 LHLKREEVRSVSPSNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEK 360
                                                S+S+ H   D+ P        EK
Sbjct: 301 -------------------------------------SASYEHTSHDL-PFDSENAGVEK 360

Query: 361 FGFKPRFEQEINSPPPSVSRTHEETNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQ 420
              K       +  P   S+T  E    SP +  + E+ N   PS+   +  G  P  E 
Sbjct: 361 TASKR------DEHPAKASKTVVEGQQSSPILMESFEKKNYSPPSI---DAVGPIPTKES 420

Query: 421 NLDSPPPSVSRTKSEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELT 480
                 P   R  S+       DLQDVL AAQAAA++AERAA+AARSAASLA++RI+ELT
Sbjct: 421 GASRDTP---RKISD------GDLQDVLMAAQAAADSAERAASAARSAASLAQLRINELT 480

Query: 481 KKKNDRDAEVSCENPFHGAAV--------------------------------------- 540
           +K +D+  E   ENPFH  ++                                       
Sbjct: 481 RKTSDQYPESPSENPFHAPSMGNLQFDHQNSSASSSGDLTELQRAETSSLFNSEQNNQQP 540

Query: 541 ---TPPDKQTF--QQDSLGNYS----MPYYSQDSFKEHHRVSC-----DL---------- 600
              +  +K  F  Q  S  +Y       ++S +  +  H+ S      DL          
Sbjct: 541 QTHSSMEKPQFDRQNSSFSSYGDLTPQRFHSMEKPQFDHQNSSGSNYDDLTPQRFSSLEK 600

Query: 601 --------------DVTGHHQLEPQKSGFD---------------SSPSNSPSERIA--H 609
                         D+T H     +K  FD                 P  SP +R++   
Sbjct: 601 PQFDHQNSSGSNYDDLTPHRFPSMEKPQFDHQNSSVSSYGDLPELQRPETSPLDRLSPDQ 617

BLAST of Carg26570 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 394.8 bits (1013), Expect = 1.2e-109
Identity = 310/734 (42.23%), Postives = 377/734 (51.36%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MSML+SFFN+GFKAA+CKTLLKLTIPRIKL+RNRRE Q+KQMRR+IAKLLETGQEATARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEHIIREE MMAAQEILELFCELI VRLPIIE QRECPLDLKEAISSVCFAAPRC+DLT
Sbjct: 61  RVEHIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLT 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNR---------------------------- 180
           EL QVQ+LF +KYGKEFV+AA+EL P+ GVNR                            
Sbjct: 121 ELQQVQILFVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNS 180

Query: 181 --QLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPEDLLNGSAQFVSAS 240
             QL+ELLSVRAPSPE KLKLLKEIAEEH+LDWDPA TE +  KS EDLL+G  QF   S
Sbjct: 181 ILQLVELLSVRAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDGPKQFGGGS 240

Query: 241 KLALPKDKHDETYSATPDLGSAPQPDSDSELDTLDFPEVPKISVSLHPPTTFDAATAQPL 300
           KL LP++++++T + T    +  + DSDSE D LDFPEVP + +   P  T         
Sbjct: 241 KLPLPEEQNEKT-NLTSLSAAKEKSDSDSEYDILDFPEVPNVLLRPTPGAT--------- 300

Query: 301 IPPPSVSPPPETDRDSFKSSGIIPEIPPQDLHLKREEVRSVSPSNDHLNVSVGEDKQFLP 360
               SV+ P     D+ K                                          
Sbjct: 301 ----SVNAP-----DAAK------------------------------------------ 360

Query: 361 FITPPSLSSSFSHRQTDVCPSSDSKTPEEKFGFKPRFEQEINSPPPSVSRTHEETNSLSP 420
                  S+S+ H   D+ P        EK   K       +  P   S+T  E    SP
Sbjct: 361 -------SASYEHTSHDL-PFDSENAGVEKTASKR------DEHPAKASKTVVEGQQSSP 420

Query: 421 SVSRTHEETNSLRPSVSPEEKFGFKPQFEQNLDSPPPSVSRTKSEVNVDVSVDLQDVLAA 480
            +  + E+ N   PS+   +  G  P  E       P   R  S+       DLQDVL A
Sbjct: 421 ILMESFEKKNYSPPSI---DAVGPIPTKESGASRDTP---RKISD------GDLQDVLMA 480

Query: 481 AQAAAETAERAAAAARSAASLAKVRIDELTKKKNDRDAEVSCENPFHGAAV--------- 540
           AQAAA++AERAA+AARSAASLA++RI+ELT+K +D+  E   ENPFH  ++         
Sbjct: 481 AQAAADSAERAASAARSAASLAQLRINELTRKTSDQYPESPSENPFHAPSMGNLQFDHQN 540

Query: 541 ---------------------------------TPPDKQTF--QQDSLGNYS----MPYY 600
                                            +  +K  F  Q  S  +Y       ++
Sbjct: 541 SSASSSGDLTELQRAETSSLFNSEQNNQQPQTHSSMEKPQFDRQNSSFSSYGDLTPQRFH 600

Query: 601 SQDSFKEHHRVSC-----DL------------------------DVTGHHQLEPQKSGFD 609
           S +  +  H+ S      DL                        D+T H     +K  FD
Sbjct: 601 SMEKPQFDHQNSSGSNYDDLTPQRFSSLEKPQFDHQNSSGSNYDDLTPHRFPSMEKPQFD 647

BLAST of Carg26570 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 243.8 bits (621), Expect = 3.5e-64
Identity = 129/238 (54.20%), Postives = 175/238 (73.53%), Query Frame = 0

Query: 6   SFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHI 65
           S F RGF +++CKT  K+ + RIKL+RN+R + +KQMRRDIA LL++GQ+ATARIRVEH+
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 66  IREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQV 125
           IRE+N+ AA EI+ELFCELIV RL II  Q++CP+DLKE I+S+ FAAPRC+++ EL  +
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 126 QLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 185
           + +FA KYGK+FVSAAT+L P+CGVNR LI+ LSVR P  E KLK++KEIA+E  +DWD 
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDT 199

Query: 186 AGTEAEFNKSPEDLLNGSAQFVSASKLALPKDKHDETYSATPDLGSAPQPDSDSELDT 244
             TE E  K  E+ ++G  +FVSAS L + +   +E    T    + P+  S   ++T
Sbjct: 200 TETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTK---AVPRSTSSMSINT 254

BLAST of Carg26570 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 241.1 bits (614), Expect = 2.3e-63
Identity = 120/205 (58.54%), Postives = 161/205 (78.54%), Query Frame = 0

Query: 1   MSMLNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARI 60
           MS+LN  FNRG   A+CKT L L I R+KLL+N+R++QLK M+++IA  L+ GQE  ARI
Sbjct: 1   MSLLNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARI 60

Query: 61  RVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLT 120
           RVEH+IRE N+ AA EILELFCE I+ R+PI+E+++ECP +L+EAI+S+ FAAPRC+++ 
Sbjct: 61  RVEHVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVP 120

Query: 121 ELLQVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHD 180
           +LLQ++ LF  KYGKEF+  A+EL P+ GVNR +IE LS  +PS   +LK+LKEIA+E+ 
Sbjct: 121 DLLQIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYS 180

Query: 181 LDWDPAGTEAEFNKSPEDLLNGSAQ 206
           L+WD + TEAEF KS EDLL G+ Q
Sbjct: 181 LNWDSSATEAEFMKSHEDLLGGAKQ 205

BLAST of Carg26570 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 202.2 bits (513), Expect = 1.2e-51
Identity = 102/209 (48.80%), Postives = 153/209 (73.21%), Query Frame = 0

Query: 4   LNSFFNRGFKAAQCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVE 63
           +     RGFK A+CKT L++   R+K+L+N++EIQ+KQ+RR++A+LLE+GQ  TARIRVE
Sbjct: 1   MKKVLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVE 60

Query: 64  HIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELL 123
           H++REE  +AA E++ ++CEL+VVRL +IE+Q+ CP+DLKEA++SV FA+ R +D+ EL 
Sbjct: 61  HVVREEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELS 120

Query: 124 QVQLLFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDW 183
           ++   F  KYGK+F ++A EL P+ GV+R L+E LS +AP    K+K+L  IAEEH++ W
Sbjct: 121 EIFKQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVW 180

Query: 184 DPAGTEAEFNKSPEDLLNGSAQFVSASKL 213
           + A +  E +    +LLNG+  F  AS +
Sbjct: 181 E-AQSFVESDPKDTELLNGANSFQPASSM 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037225.10.0e+00100.00IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022926259.10.0e+0099.18uncharacterized protein LOC111433438 [Cucurbita moschata][more]
XP_023521005.10.0e+0098.03uncharacterized protein LOC111784587 [Cucurbita pepo subsp. pepo][more]
KAG6607619.10.0e+0097.70IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022981875.10.0e+0097.54uncharacterized protein LOC111480885 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P539901.1e-2037.50IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX001.1e-2037.50IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q568Z61.1e-2037.50IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
Q3ZBV15.2e-2036.93IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
Q5R6G86.8e-2036.93IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EEM30.0e+0099.18uncharacterized protein LOC111433438 OS=Cucurbita moschata OX=3662 GN=LOC1114334... [more]
A0A6J1J0W10.0e+0097.54uncharacterized protein LOC111480885 OS=Cucurbita maxima OX=3661 GN=LOC111480885... [more]
A0A0A0KBZ03.3e-24376.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G020110 PE=3 SV=1[more]
A0A1S4E4M84.4e-24076.42uncharacterized protein LOC103502259 OS=Cucumis melo OX=3656 GN=LOC103502259 PE=... [more]
A0A6J1EU691.8e-22873.98uncharacterized protein LOC111436655 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G34220.26.4e-11443.89Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.11.2e-10942.23Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.13.5e-6454.20Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.12.3e-6358.54Regulator of Vps4 activity in the MVB pathway protein [more]
AT2G19710.11.2e-5148.80Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 448..468
NoneNo IPR availableCOILSCoilCoilcoord: 32..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 333..352
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 495..514
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 547..568
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 584..601
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 372..406
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 217..320
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 333..434
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 543..609
NoneNo IPR availablePANTHERPTHR12161:SF62REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 1..598
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 18..181
e-value: 6.6E-59
score: 198.5
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 1..598
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 5..192
e-value: 3.9E-78
score: 263.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26570-RACarg26570-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
molecular_function GO:0003676 nucleic acid binding