Carg26525 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26525
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein HEAT-STRESS-ASSOCIATED 32
Location	Carg_Chr18: 1848252 .. 1850662 (+)
RNA-Seq Expression	Carg26525
Synteny	Carg26525

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGAAGGAGATGGGAGAATTACTCTGGTTCTGATTCAACCATCACTCCCTTGGAGGAAATGTCTGCTTATTGGTGGAAGACCTTCGCTGGGGATGAAGATCGACCGGAGAAGCCTCGGCGATGTGGAGTTACAGAGATGAGAAGTCCTCATTACTCTCTCTCTAGCCAAGATGTGATTCAGGTTCGTACGTTATATATCTTGTTCTTTGATTCCACGCTTTTGGTTTGAAATCGAACACGCTTGATGCTTAGGCTGTACTGGAAGATGATTTCGGCTTATTGATCGCTGTTTCTTTAATATGATTGCAAAAATTGATGTTATGAAGGAAATTTTCGAGTCGATGGGTCAATTTGTCGATGGATTAAAGTTCTCCGGAGGTTCGCACAGCCTGTTGCCGAGGGAGTTTGTGAAAGAGGTGACGGAAATGGCTCATCGACATGACATTTATGTCAGTACGGGTGACTGGGCGGAGCATTTACTTCGCAAAGGTCCGTCGGGTTTCAAAGAGTATGTGGAGGTGAGAGTAAGAGAAATGCCATTAATCTTCTTGATTTTTGTTTTGGGATCGAAGTTGGAGTGATAATTAGGAGTATGGTGTCTCTAGGAATGCAAGCTCATGGGATTTGATACTATTGAGCTTAACGTAGACTCGCTTGAAGTTCCTGAAGAAACTCTTCTGAGATATGTGCGCTTAATTAAGAACGGTGGCCTTAGGGCTAAGCCTCAATTTGCAGTGAAGTTCAATAAGTCGGACATTCCCGCTGGCGATCGAGCATTTGGAGCTTATATTGTTCCAAGACCTCGATCTTCTGGTAAGCTTTCAATTGCAAATCTCTTCGATTTCTTTATTCTAGGGCTAGGACTTACTTTTGGATTGTGAAAAAAAACCAGCCCGATACACATGTTGATTGCTTTAAATTTGTCATTTGTGAGATGATCACTCTTGATTGCTCTAAATTGTTCTTGGTTTCACCTTGATTAATTTTGGATTGTGAAAAAACCAGCCTGATACACATCTTGATTGCTTTAAATTTGTCATTTGTAAGATGATCACTCTTGATTGCTCTAAATTGTTCTTGGTTTCATCTTGATTACTTTTGGATTGTGAAAAAAACCAGCCTGATACACATGTTGATTGCTTTAAACATTTGTAAGATGATCACTCTTGATTGCTCTAAATTGTTCTTGGTTTCATCTTGATTACTTGTGGATTGTGAAAAAACCAGCCTGATACACATCTTGATTGCTTTAAATTTGTCATTTGTAAGATGATCACTCTTGATTGCTCTAAGTTGTTCTTGGGTTCATCATGCTGGCATTTGATCCTACTACATAACTCTTGATATGGTGATTCTCTCTTGGCTTGCAATTTACTAGAGATGAAACTGTTGTTCATAATTGTTGCTCTAGTTCTATTTTACTTTTGCATGCTTGATGTCATGATTGGGTCCATTTCATAACCTTTGATGGGTGATGGATATAACGAATGTGGAATCAATGCTAGTGTTCATGGTGCAATAAGTGCAATATATATCTCTTGCTTCAATCATATAAAGCCTCTTTGTTGGTGTTGGACTGTTGGTACCCTTTCTGAATCTCTCAAGGCCATTCAACTAGTTTAACTTCCATTGACAAGTAATGATCGGTTAGGAATCACGGACCCTCCACAATGGTATAATATTATCCACTTTGAGCATAAGCTCTCGTGGTTTTGCTTTTGGTTTCTCCAAAAGGCCTCATACCAATGGAGATGTATTCCTTATAAATCCATGATCAACCCTTTAATTAGCCGATGCAAGACTCGTCTCCCAACAATCCTTGACATGATCTCTATACTTTTAAATCTTGAGTTTTTATCACGATAACTTGTTGCAGAACTTGTAGAGGATGTTGATCTTTTGATCAGAAGGGCTGAGAGATGCCTAGAAGCTGGGGCAGACATGATAATGATCGACGCAGACGATGTCAGTAAACACACTGATTCTCTTCGAACAGACATTATTGCCAAGATCATTGGACGTCTTGGCCTAGAGAAAACAATGTTTGAAGCATCGAATCCCCAAATGTTGGAGTGGTTTATCAATCAATACAGCTCAAAGGTATGGAAACAATATCTTCATACGGTTGCATCTACATTCCTTTACTCCATGACTGAAAACACATTCTTGTTTGCCCTGTAATTAGGTGAATCTCTTTGTGGATCACTCCCAAGTTATGGATCTTGAGTGCCTCCGAGGCCGCAACCTGGGTAAAAACCACTCCTCCGTTCTTGGTTCATCCTACTTTTAAACTCCAGAGATTTATGTTCAAAATCGTATTGTTGCAGTGTGTTTTGGTGCTTAAAATGATCCATTCAAAAACTACTTTTGTAAGAATCTCTGTCAATAAATAAATTTTGTTTTTTAAAGC

mRNA sequence

GAGGAAGGAGATGGGAGAATTACTCTGGTTCTGATTCAACCATCACTCCCTTGGAGGAAATGTCTGCTTATTGGTGGAAGACCTTCGCTGGGGATGAAGATCGACCGGAGAAGCCTCGGCGATGTGGAGTTACAGAGATGAGAAGTCCTCATTACTCTCTCTCTAGCCAAGATGTGATTCAGGAAATTTTCGAGTCGATGGGTCAATTTGTCGATGGATTAAAGTTCTCCGGAGGTTCGCACAGCCTGTTGCCGAGGGAGTTTGTGAAAGAGGTGACGGAAATGGCTCATCGACATGACATTTATGTCAGTACGGGTGACTGGGCGGAGCATTTACTTCGCAAAGGTCCGTCGGGTTTCAAAGAGTATGTGGAGGAATGCAAGCTCATGGGATTTGATACTATTGAGCTTAACGTAGACTCGCTTGAAGTTCCTGAAGAAACTCTTCTGAGATATGTGCGCTTAATTAAGAACGGTGGCCTTAGGGCTAAGCCTCAATTTGCAGTGAAGTTCAATAAGTCGGACATTCCCGCTGGCGATCGAGCATTTGGAGCTTATATTGTTCCAAGACCTCGATCTTCTGAACTTGTAGAGGATGTTGATCTTTTGATCAGAAGGGCTGAGAGATGCCTAGAAGCTGGGGCAGACATGATAATGATCGACGCAGACGATGTCAGTAAACACACTGATTCTCTTCGAACAGACATTATTGCCAAGATCATTGGACGTCTTGGCCTAGAGAAAACAATGTTTGAAGCATCGAATCCCCAAATGTTGGAGTGGTTTATCAATCAATACAGCTCAAAGGTGAATCTCTTTGTGGATCACTCCCAAGTTATGGATCTTGAGTGCCTCCGAGGCCGCAACCTGGGTAAAAACCACTCCTCCGTTCTTGGTTCATCCTACTTTTAAACTCCAGAGATTTATGTTCAAAATCGTATTGTTGCAGTGTGTTTTGGTGCTTAAAATGATCCATTCAAAAACTACTTTTGTAAGAATCTCTGTCAATAAATAAATTTTGTTTTTTAAAGC

Coding sequence (CDS)

ATGTCTGCTTATTGGTGGAAGACCTTCGCTGGGGATGAAGATCGACCGGAGAAGCCTCGGCGATGTGGAGTTACAGAGATGAGAAGTCCTCATTACTCTCTCTCTAGCCAAGATGTGATTCAGGAAATTTTCGAGTCGATGGGTCAATTTGTCGATGGATTAAAGTTCTCCGGAGGTTCGCACAGCCTGTTGCCGAGGGAGTTTGTGAAAGAGGTGACGGAAATGGCTCATCGACATGACATTTATGTCAGTACGGGTGACTGGGCGGAGCATTTACTTCGCAAAGGTCCGTCGGGTTTCAAAGAGTATGTGGAGGAATGCAAGCTCATGGGATTTGATACTATTGAGCTTAACGTAGACTCGCTTGAAGTTCCTGAAGAAACTCTTCTGAGATATGTGCGCTTAATTAAGAACGGTGGCCTTAGGGCTAAGCCTCAATTTGCAGTGAAGTTCAATAAGTCGGACATTCCCGCTGGCGATCGAGCATTTGGAGCTTATATTGTTCCAAGACCTCGATCTTCTGAACTTGTAGAGGATGTTGATCTTTTGATCAGAAGGGCTGAGAGATGCCTAGAAGCTGGGGCAGACATGATAATGATCGACGCAGACGATGTCAGTAAACACACTGATTCTCTTCGAACAGACATTATTGCCAAGATCATTGGACGTCTTGGCCTAGAGAAAACAATGTTTGAAGCATCGAATCCCCAAATGTTGGAGTGGTTTATCAATCAATACAGCTCAAAGGTGAATCTCTTTGTGGATCACTCCCAAGTTATGGATCTTGAGTGCCTCCGAGGCCGCAACCTGGGTAAAAACCACTCCTCCGTTCTTGGTTCATCCTACTTTTAA

Protein sequence

MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGSHSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVDSLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDVDLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLEWFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF

Homology

[Close]

BLAST of Carg26525 vs. NCBI nr
Match: KAG7012394.1 (Protein HEAT-STRESS-ASSOCIATED 32 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.0 bits (1499), Expect = 2.7e-162
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS
Sbjct: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD
Sbjct: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 180
           SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV
Sbjct: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 180

Query: 181 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE 240
           DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE
Sbjct: 181 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE 240

Query: 241 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF
Sbjct: 241 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 283

[Close]

BLAST of Carg26525 vs. NCBI nr
Match: XP_022954632.1 (protein HEAT-STRESS-ASSOCIATED 32 [Cucurbita moschata])

HSP 1 Score: 580.9 bits (1496), Expect = 6.1e-162
Identity = 282/283 (99.65%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQD+IQEIFESMGQFVDGLKFSGGS
Sbjct: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDMIQEIFESMGQFVDGLKFSGGS 60

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD
Sbjct: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 180
           SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV
Sbjct: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 180

Query: 181 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE 240
           DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE
Sbjct: 181 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE 240

Query: 241 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF
Sbjct: 241 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 283

[Close]

BLAST of Carg26525 vs. NCBI nr
Match: XP_023542719.1 (protein HEAT-STRESS-ASSOCIATED 32 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.4 bits (1474), Expect = 2.2e-159
Identity = 277/283 (97.88%), Postives = 281/283 (99.29%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQD+IQEIFESMGQFVDGLKFSGGS
Sbjct: 38  MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDMIQEIFESMGQFVDGLKFSGGS 97

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGF+EYVEECKLMGFDTIELNVD
Sbjct: 98  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFREYVEECKLMGFDTIELNVD 157

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 180
           SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYI+PRPRSSELVEDV
Sbjct: 158 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYILPRPRSSELVEDV 217

Query: 181 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE 240
           DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNP+ LE
Sbjct: 218 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPKTLE 277

Query: 241 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVL SSYF
Sbjct: 278 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLASSYF 320

[Close]

BLAST of Carg26525 vs. NCBI nr
Match: XP_022994429.1 (protein HEAT-STRESS-ASSOCIATED 32 [Cucurbita maxima])

HSP 1 Score: 570.5 bits (1469), Expect = 8.2e-159
Identity = 276/283 (97.53%), Postives = 281/283 (99.29%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHY+LSSQ+VIQEIFESMGQFVDGLKFSGGS
Sbjct: 38  MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYTLSSQNVIQEIFESMGQFVDGLKFSGGS 97

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD
Sbjct: 98  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 157

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 180
           SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV
Sbjct: 158 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 217

Query: 181 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE 240
           DLLIRRAERCLEAGADMIMIDADDVSKHTDSLR DIIAKIIGRLGLEKTMFEASNP+ LE
Sbjct: 218 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRADIIAKIIGRLGLEKTMFEASNPKTLE 277

Query: 241 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           WFINQYSSKVNLFVDHSQVM+LECLRGRNLGKNHSSVLGS+YF
Sbjct: 278 WFINQYSSKVNLFVDHSQVMELECLRGRNLGKNHSSVLGSAYF 320

[Close]

BLAST of Carg26525 vs. NCBI nr
Match: XP_038893901.1 (protein HEAT-STRESS-ASSOCIATED 32 [Benincasa hispida])

HSP 1 Score: 533.5 bits (1373), Expect = 1.1e-147
Identity = 260/283 (91.87%), Postives = 271/283 (95.76%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAY WK+F  DEDRPEKPRRCGVTEMR PHY+LSSQ+VIQEIFES+GQFVDGLKFSGGS
Sbjct: 1   MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGS 60

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPR+F+KEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKL+GFDTIELNV 
Sbjct: 61  HSLLPRDFMKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP-AGDRAFGAYIVPRPRSSELVED 180
           SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP +G+RAFGAYIVPRPRSSELVED
Sbjct: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQML 240
           VDLLIRRAERCLEAGADMIMIDADDVSKH +SLRTDIIAKIIGRLGLEKTMFEASNPQ  
Sbjct: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTS 240

Query: 241 EWFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSY 283
           EWFIN Y  KVNLFVDHSQVMDLECLRGRNLGKNHSS+LGSSY
Sbjct: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSY 283

[Close]

BLAST of Carg26525 vs. ExPASy Swiss-Prot
Match: Q8GWL1 (Protein HEAT-STRESS-ASSOCIATED 32 OS=Arabidopsis thaliana OX=3702 GN=HSA32 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 6.4e-130
Identity = 217/284 (76.41%), Postives = 252/284 (88.73%), Query Frame = 0


Query: 1   MSAYW-WKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGG 60
           M+AY+ WK+F  +EDRPEKPRR GVTEMR PHYS+ SQ+++QEIFESMGQFVDGLKFSGG
Sbjct: 1   MAAYYRWKSFEENEDRPEKPRRYGVTEMRGPHYSVLSQNLLQEIFESMGQFVDGLKFSGG 60

Query: 61  SHSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNV 120
           S+SL+P+ F+K+  EMAH H +YVSTGDWAEH+LR GPS FK+YVEECK +GFDTIELN 
Sbjct: 61  SNSLIPKSFIKQAIEMAHEHGVYVSTGDWAEHMLRSGPSAFKDYVEECKQLGFDTIELNA 120

Query: 121 DSLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVED 180
           + LEVPEETLLRYVRLIKNGGLRAKP FAVKFNKSDIP  +RAFG+Y+VP PRSSE VED
Sbjct: 121 NLLEVPEETLLRYVRLIKNGGLRAKPMFAVKFNKSDIPGRNRAFGSYVVPEPRSSEFVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQML 240
           +DLLIR+AERCLEAGAD IMIDADDV K+ DSLR DIIAK+IGRLG+EKTMFEAS+ +++
Sbjct: 181 IDLLIRKAERCLEAGADTIMIDADDVCKYADSLRADIIAKVIGRLGIEKTMFEASDAKLV 240

Query: 241 EWFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           EWFI +Y   VNL+VDHSQ+MDLECLRGR+LGK+H SVL SSYF
Sbjct: 241 EWFIKRYGPNVNLYVDHSQIMDLECLRGRHLGKDHQSVLSSSYF 284

[Close]

BLAST of Carg26525 vs. ExPASy Swiss-Prot
Match: O27710 (Phosphosulfolactate synthase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=comA PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.7e-11
Identity = 63/269 (23.42%), Postives = 113/269 (42.01%), Query Frame = 0


Query: 15  RPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGSHSLLPREFVKEVTE 74
           R  KPR+ G+T +       +S    +++ E    +VD +KF  G+  L  R+ VKE  +
Sbjct: 11  RSGKPRKNGITMVLDKGMGPAS---ARDLMEISSDYVDFIKFGWGTLPLHRRDTVKEKVD 70

Query: 75  MAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVDSLEVPEETLLRYVR 134
           M    D+    G     +        +EY +E + +GF+T+E++  ++E+  E   R + 
Sbjct: 71  MYRSFDVEPYPGGTLFEIAHLNDK-VEEYFQEARSLGFETLEISNGTVEIETEEKCRLIE 130

Query: 135 LIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDVDLLIRRAERCLEAG 194
           +  + G     +   K                    P    L+E  D  +R     L AG
Sbjct: 131 MAVDEGFMVLSEVGKK-------------------DPERDRLLEPDD-RVRLVRADLRAG 190

Query: 195 ADMIMIDADD------VSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLEWFINQYSS 254
           A M++++A +      +     ++R D    +  RL +++ ++EA       +FI +   
Sbjct: 191 ASMVLMEARESGQNIGIYDERGNIREDEFNHLTDRLPMDRIIWEAPQKSQQVYFILKIGP 250

Query: 255 KVNL-FVDHSQVMDLEC----LRGRNLGK 273
            VNL  +   ++  LE     LRG  LGK
Sbjct: 251 DVNLGNIPPEEITALETIRRGLRGDTLGK 255

[Close]

BLAST of Carg26525 vs. ExPASy TrEMBL
Match: A0A6J1GRH3 (protein HEAT-STRESS-ASSOCIATED 32 OS=Cucurbita moschata OX=3662 GN=LOC111456838 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.0e-162
Identity = 282/283 (99.65%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQD+IQEIFESMGQFVDGLKFSGGS
Sbjct: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDMIQEIFESMGQFVDGLKFSGGS 60

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD
Sbjct: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 180
           SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV
Sbjct: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 180

Query: 181 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE 240
           DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE
Sbjct: 181 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE 240

Query: 241 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF
Sbjct: 241 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 283

[Close]

BLAST of Carg26525 vs. ExPASy TrEMBL
Match: A0A6J1K2U1 (protein HEAT-STRESS-ASSOCIATED 32 OS=Cucurbita maxima OX=3661 GN=LOC111490151 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 4.0e-159
Identity = 276/283 (97.53%), Postives = 281/283 (99.29%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHY+LSSQ+VIQEIFESMGQFVDGLKFSGGS
Sbjct: 38  MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYTLSSQNVIQEIFESMGQFVDGLKFSGGS 97

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD
Sbjct: 98  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 157

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 180
           SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV
Sbjct: 158 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVEDV 217

Query: 181 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQMLE 240
           DLLIRRAERCLEAGADMIMIDADDVSKHTDSLR DIIAKIIGRLGLEKTMFEASNP+ LE
Sbjct: 218 DLLIRRAERCLEAGADMIMIDADDVSKHTDSLRADIIAKIIGRLGLEKTMFEASNPKTLE 277

Query: 241 WFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           WFINQYSSKVNLFVDHSQVM+LECLRGRNLGKNHSSVLGS+YF
Sbjct: 278 WFINQYSSKVNLFVDHSQVMELECLRGRNLGKNHSSVLGSAYF 320

[Close]

BLAST of Carg26525 vs. ExPASy TrEMBL
Match: A0A0A0LUX3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G066510 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 4.6e-147
Identity = 256/284 (90.14%), Postives = 270/284 (95.07%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAY WK+F  DEDRPEKPRRCGVTEMR PHY+LS+QDVIQEIFES+GQFVDGLKFSGGS
Sbjct: 1   MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSTQDVIQEIFESLGQFVDGLKFSGGS 60

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPR+F+KEVTEMAHRHD+YVSTGDWAEHLLRKGPSGFKEYVEECKL+GFDTIELNV 
Sbjct: 61  HSLLPRDFMKEVTEMAHRHDVYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP-AGDRAFGAYIVPRPRSSELVED 180
           SL+VPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP +G+RAFGAYIVP+PRSSELVED
Sbjct: 121 SLDVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPISGNRAFGAYIVPKPRSSELVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQML 240
           VDLLIRRAERCLEAGADMIMIDADDVSKH + LR DIIAKIIGRLGLEKTMFEASNPQ  
Sbjct: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHAEFLRADIIAKIIGRLGLEKTMFEASNPQTS 240

Query: 241 EWFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           EWFIN Y  KVNLFVDHSQVMDLECLRGRNLGKNHSS+LGSSYF
Sbjct: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYF 284

[Close]

BLAST of Carg26525 vs. ExPASy TrEMBL
Match: A0A5A7UNV6 (Protein HEAT-STRESS-ASSOCIATED 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006250 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.6e-145
Identity = 253/284 (89.08%), Postives = 268/284 (94.37%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAY WK+F  DEDRPEKPRR GVTEMR PHY+LS+Q+VIQEIFES+GQFVDGLKFSGGS
Sbjct: 1   MSAYLWKSFDEDEDRPEKPRRSGVTEMRGPHYTLSTQNVIQEIFESVGQFVDGLKFSGGS 60

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPR+F+KEVTEMAHRHD+YVSTGDWAEHLLRKGPSGFKEYVEECKL+GFDTIELNV 
Sbjct: 61  HSLLPRDFMKEVTEMAHRHDVYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP-AGDRAFGAYIVPRPRSSELVED 180
           SL+VPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP  G+RAFGAYIVP+PRSSE VED
Sbjct: 121 SLDVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPITGNRAFGAYIVPKPRSSEFVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQML 240
           VDLLIRRAERCLEAGADMIMIDADDVS+H +SLR DIIAKIIGRLGLEKTMFEASNPQ  
Sbjct: 181 VDLLIRRAERCLEAGADMIMIDADDVSRHAESLRADIIAKIIGRLGLEKTMFEASNPQTS 240

Query: 241 EWFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           EWFIN Y  KVNLFVDHSQVMDLECLRGRNLGKNHSS+LGSSYF
Sbjct: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYF 284

[Close]

BLAST of Carg26525 vs. ExPASy TrEMBL
Match: A0A1S3B4M6 (protein HEAT-STRESS-ASSOCIATED 32 OS=Cucumis melo OX=3656 GN=LOC103485727 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.6e-145
Identity = 253/284 (89.08%), Postives = 268/284 (94.37%), Query Frame = 0


Query: 1   MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGGS 60
           MSAY WK+F  DEDRPEKPRR GVTEMR PHY+LS+Q+VIQEIFES+GQFVDGLKFSGGS
Sbjct: 1   MSAYLWKSFDEDEDRPEKPRRSGVTEMRGPHYTLSTQNVIQEIFESVGQFVDGLKFSGGS 60

Query: 61  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 120
           HSLLPR+F+KEVTEMAHRHD+YVSTGDWAEHLLRKGPSGFKEYVEECKL+GFDTIELNV 
Sbjct: 61  HSLLPRDFMKEVTEMAHRHDVYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP-AGDRAFGAYIVPRPRSSELVED 180
           SL+VPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP  G+RAFGAYIVP+PRSSE VED
Sbjct: 121 SLDVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPITGNRAFGAYIVPKPRSSEFVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQML 240
           VDLLIRRAERCLEAGADMIMIDADDVS+H +SLR DIIAKIIGRLGLEKTMFEASNPQ  
Sbjct: 181 VDLLIRRAERCLEAGADMIMIDADDVSRHAESLRADIIAKIIGRLGLEKTMFEASNPQTS 240

Query: 241 EWFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           EWFIN Y  KVNLFVDHSQVMDLECLRGRNLGKNHSS+LGSSYF
Sbjct: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYF 284

[Close]

BLAST of Carg26525 vs. TAIR 10
Match: AT4G21320.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 464.9 bits (1195), Expect = 4.5e-131
Identity = 217/284 (76.41%), Postives = 252/284 (88.73%), Query Frame = 0


Query: 1   MSAYW-WKTFAGDEDRPEKPRRCGVTEMRSPHYSLSSQDVIQEIFESMGQFVDGLKFSGG 60
           M+AY+ WK+F  +EDRPEKPRR GVTEMR PHYS+ SQ+++QEIFESMGQFVDGLKFSGG
Sbjct: 1   MAAYYRWKSFEENEDRPEKPRRYGVTEMRGPHYSVLSQNLLQEIFESMGQFVDGLKFSGG 60

Query: 61  SHSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNV 120
           S+SL+P+ F+K+  EMAH H +YVSTGDWAEH+LR GPS FK+YVEECK +GFDTIELN 
Sbjct: 61  SNSLIPKSFIKQAIEMAHEHGVYVSTGDWAEHMLRSGPSAFKDYVEECKQLGFDTIELNA 120

Query: 121 DSLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPAGDRAFGAYIVPRPRSSELVED 180
           + LEVPEETLLRYVRLIKNGGLRAKP FAVKFNKSDIP  +RAFG+Y+VP PRSSE VED
Sbjct: 121 NLLEVPEETLLRYVRLIKNGGLRAKPMFAVKFNKSDIPGRNRAFGSYVVPEPRSSEFVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHTDSLRTDIIAKIIGRLGLEKTMFEASNPQML 240
           +DLLIR+AERCLEAGAD IMIDADDV K+ DSLR DIIAK+IGRLG+EKTMFEAS+ +++
Sbjct: 181 IDLLIRKAERCLEAGADTIMIDADDVCKYADSLRADIIAKVIGRLGIEKTMFEASDAKLV 240

Query: 241 EWFINQYSSKVNLFVDHSQVMDLECLRGRNLGKNHSSVLGSSYF 284
           EWFI +Y   VNL+VDHSQ+MDLECLRGR+LGK+H SVL SSYF
Sbjct: 241 EWFIKRYGPNVNLYVDHSQIMDLECLRGRHLGKDHQSVLSSSYF 284

The following BLAST results are available for this feature:

BLAST of Carg26525 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012394.1	2.7e-162	100.00	Protein HEAT-STRESS-ASSOCIATED 32 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022954632.1	6.1e-162	99.65	protein HEAT-STRESS-ASSOCIATED 32 [Cucurbita moschata]	[more]
XP_023542719.1	2.2e-159	97.88	protein HEAT-STRESS-ASSOCIATED 32 [Cucurbita pepo subsp. pepo]	[more]
XP_022994429.1	8.2e-159	97.53	protein HEAT-STRESS-ASSOCIATED 32 [Cucurbita maxima]	[more]
XP_038893901.1	1.1e-147	91.87	protein HEAT-STRESS-ASSOCIATED 32 [Benincasa hispida]	[more]

BLAST of Carg26525 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GWL1	6.4e-130	76.41	Protein HEAT-STRESS-ASSOCIATED 32 OS=Arabidopsis thaliana OX=3702 GN=HSA32 PE=2 ...	[more]
O27710	2.7e-11	23.42	Phosphosulfolactate synthase OS=Methanothermobacter thermautotrophicus (strain A...	[more]

BLAST of Carg26525 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GRH3	3.0e-162	99.65	protein HEAT-STRESS-ASSOCIATED 32 OS=Cucurbita moschata OX=3662 GN=LOC111456838 ...	[more]
A0A6J1K2U1	4.0e-159	97.53	protein HEAT-STRESS-ASSOCIATED 32 OS=Cucurbita maxima OX=3661 GN=LOC111490151 PE...	[more]
A0A0A0LUX3	4.6e-147	90.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G066510 PE=3 SV=1	[more]
A0A5A7UNV6	5.6e-145	89.08	Protein HEAT-STRESS-ASSOCIATED 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3B4M6	5.6e-145	89.08	protein HEAT-STRESS-ASSOCIATED 32 OS=Cucumis melo OX=3656 GN=LOC103485727 PE=3 S...	[more]

BLAST of Carg26525 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21320.1	4.5e-131	76.41	Aldolase-type TIM barrel family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003830	(2R)-phospho-3-sulpholactate synthase, ComA	PFAM	PF02679	ComA	coord: 14..270 e-value: 2.0E-78 score: 263.2
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 12..273 e-value: 6.4E-79 score: 266.7
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 14..283
None	No IPR available	PANTHER	PTHR33116:SF50	PROTEIN HEAT-STRESS-ASSOCIATED 32	coord: 14..283
IPR036112	(2R)-phospho-3-sulpholactate synthase, ComA superfamily	SUPERFAMILY	102110	(2r)-phospho-3-sulfolactate synthase ComA	coord: 13..266

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26525-RA	Carg26525-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010286	heat acclimation
biological_process	GO:0010608	posttranscriptional regulation of gene expression
molecular_function	GO:0003824	catalytic activity