Carg26523 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26523
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description(3S,6E)-nerolidol synthase 1
LocationCarg_Chr18: 1861889 .. 1864802 (+)
RNA-Seq ExpressionCarg26523
SyntenyCarg26523
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGAAACAAAGATCCAGCAACGCCCTATTAATCCTCCTCAAAACCCCTTTCCAACAACATGGAGCGATCTAGATGACCCATTGGAAGCTTTAAGCTTGATCGATACGGCTCACCGTCTCGGCATCGCCTACCGTTTCCAAGCCGAGGTCGACCTTATTTTACAACGCCATTACGCAGCCTCACTCAACCTCGACGTTGCTTATGCAAACCACGAACTTTATGAAGTCGCTCTTCGCTTTCGACTCCTCCGACAAGGTGGCTACTTTGTGCCGTCGGACATGTTCGAGGCTTTCATGGACGAGGATGGCCATTTCAAGCAGGAGCTTGAGAATGATATCAATGGGGTGATGAGTTTGTTTGAAGCTTCGCAATTTTGCTTGCCAGGCGAAGCTATACTCGAGGAAGCTCGAGTTTTTAGCGCTCGAATCATGAGTGAATACGTCATGAACAATGTTGATTGTAAAAAAGCTAGGCATGTTGCTAGAGCTTTGGCGAATCCTTATCATACGAGCTTCTCTAAGTTCTTGGTGAAGGATTATTTTGGAATGGGGGATTTACCGGACACGAATAGATTGACCCGTGATTTCCAACTTGTCGCCAAAATGGATTTCAACGTTGCCCAGGACATGCGTCGTCGGGAACTTTATCGGTTCACACAGTAAGTTCGACCGAATTAATATTGAGGATTGTTGGGAGGGAGTCTTACATTGGCTATCGTGGGTTTATAAGTAAGGAGTACATCTTCATTGGTATGAAGTATTTTGGGAAAGCCGAGAGTAAAGTCATGAGAGCTTATGCTCAAAGTGGACAATATCATACTATTGTGGAGAGTCATGGTTTCTAATATGGTATCAGAGTCATGCTCTTAACTTAGCCATGTCAATAGAATCCTCAAATGTCGAACAAAGAGTGTACTTTGTTCGAGGACTCTAGAGAAGGAGTTGAGCCCTGTTCGAGTGCTCCATAGGCCTCAAGGAAAGCTCTACAATGTACTTTGTTCGAGAGGAAAATTGTTAGGAAGGAGTGGACAATAGAGTCATGTTCCTAACAATTAACCGACCAAATTATGTTATCCTATACTAACGTTAAACTAATGTCATGTTGGTTGTAGATGGTGGACAGACACAGGTTTGGGCAAAGTGTTGGGATTTGCAAGGGACCAACCGCTAAAATGGTACATTTGTTCGTTAGTTTGCCTCACAGATCCATGTTTTTCGGAGGAAAGAGTTGAACTTTCAAAACCCATCTCTTTTATTTATCTTATTGACGACATGTTCGATGTTTACGCCTCGCTCGACCAACTTAAGCTGTTCACAGAAGCTGTTAGAAGGTATGAACTCAAATTCAAATTCAAATTCAAATTCATTGTATGTTGATTTCAACAGACCTGAAAACTTATATTCCCCATATTTGTTGCTGCAGATGGGATCTTACGGTGGCTGAAGCACTACCAGACTGTATGAGGATTTGTTTCAAATCACTCTATGAAATGACAAATGAAATTAGTAGTAAAATCCATAAGAAGCATGGTTGGAATCCAATCAACTCCTTAAAAAAATCGGTAATTATATTCTTACTCAACTCATTAATTTGTTGAAGATCGTTGGGAGGGAGTCCCACGTTGGCTAATTAAGAAGATAATCATCGGTTTATAAGTAAAGACTACAACTCGATTGGTACAGGGCCTTTTGGGAATAAAAGTAGAACCATGAGAGCTTACGCTCAAAGTGGATAATATCATACCATTGTGGAGAGTTCATAATTCCTAATATAGTATTAGAGTCATGCCCTTAATGTAGCAATGTCAATAGAATCTTCAGTGTCGAACAAAAAAAATTAAGACCCTTGGAGGTGTAGTAAAAAATGACTAAGGATTGAACAAAGGGTGTACTTTATTCAAAGATACTATTGAGGAGGGTCTAGATTCCTAACATGGTATTAGAGGCATGCTCGTGTACTTTGTTTGAAGGCTCCAGAGAAAGTAGTCGAGCCTCGATTAAGGGGTGACAGTTCAAGGGCTCTATAGGCCTCAGAGGAGGAGATTGTTCGAGGGCTCCATATTTCTCAGGAAGGCTCTATGGTATAGTTTTTTTGACGGGAGGATCGTTGGAAGAGAGTCTCACATTGGCTAATTAAGAAGATGATCATAGATCATACGAAATACATCTCACGACTCAAAATGTGTGGAGACCCGTGTGTGAAGGGTCTATGATTCCTAACCTAATTCGTGTTCCTTAATTATTTATTTATATTAATCCATTATTTTGACTATTTTTGTAGTGGGCCAAGCTTTGCGACGCATTCCTAGTAGAAAGTGAATGGTTCGTTTCTGGTTATTCACCAAGTGCCAAAGAGTATCTAAGAAATGGAGCAGTGAGCAGTGGCGTGCACGTAGTGTTAGTGCATGCGTTCTTCTTGTTAGGACAAAGAATAAACCATGAAACCATGAAACCGTGAAGCTTTTAGACAACGACCCTGAAATTATTTCTTCTACCGCAACAATCTTGAGGCTTCAAGACGACTTAGGAAGCGCCAAGGTTCGTACAAATCGATCGATGAATCGATATTCTTCTCTAATTTACTAGATCATGATGAGTTTAACTTTCTTTATGAACTTAGGATGAGAATCAAGACGGATATGATGGATCATACGTGAATTACTACATGAAAGACAATAAAGATGCTTCGATTGAGAGCTCACGACAACACATAGCCAATTTGATCTCGCATGCATGGAAGAAGCTCAATAGAGAATGTTTGTCGTTGAGTCCATTTCCTCTTGCGTTCATGGAGATTTCTTTGAATATTGCGAGATTTGTTCCGATTTTATATGGTTATGATGAAAATCAAAACCTTCCAACGCTTAAGAAGCTTGTGAAATCGATGTTGTATGAAAGAGCGGAGATATAA

mRNA sequence

ATGGCCGAAACAAAGATCCAGCAACGCCCTATTAATCCTCCTCAAAACCCCTTTCCAACAACATGGAGCGATCTAGATGACCCATTGGAAGCTTTAAGCTTGATCGATACGGCTCACCGTCTCGGCATCGCCTACCGTTTCCAAGCCGAGGTCGACCTTATTTTACAACGCCATTACGCAGCCTCACTCAACCTCGACGTTGCTTATGCAAACCACGAACTTTATGAAGTCGCTCTTCGCTTTCGACTCCTCCGACAAGGTGGCTACTTTGTGCCGTCGGACATGTTCGAGGCTTTCATGGACGAGGATGGCCATTTCAAGCAGGAGCTTGAGAATGATATCAATGGGGTGATGAGTTTGTTTGAAGCTTCGCAATTTTGCTTGCCAGGCGAAGCTATACTCGAGGAAGCTCGAGTTTTTAGCGCTCGAATCATGAGTGAATACGTCATGAACAATGTTGATTGTAAAAAAGCTAGGCATGTTGCTAGAGCTTTGGCGAATCCTTATCATACGAGCTTCTCTAAGTTCTTGGTGAAGGATTATTTTGGAATGGGGGATTTACCGGACACGAATAGATTGACCCGTGATTTCCAACTTGTCGCCAAAATGGATTTCAACGTTGCCCAGGACATGCGTCGTCGGGAACTTTATCGGTTCACACAATGGTGGACAGACACAGGTTTGGGCAAAGTGTTGGGATTTGCAAGGGACCAACCGCTAAAATGGTACATTTGTTCGTTAGTTTGCCTCACAGATCCATGTTTTTCGGAGGAAAGAGTTGAACTTTCAAAACCCATCTCTTTTATTTATCTTATTGACGACATGTTCGATGTTTACGCCTCGCTCGACCAACTTAAGCTGTTCACAGAAGCTGTTAGAAGATGGGATCTTACGGTGGCTGAAGCACTACCAGACTGTATGAGGATTTGTTTCAAATCACTCTATGAAATGACAAATGAAATTAGTAGTAAAATCCATAAGAAGCATGGTTGGAATCCAATCAACTCCTTAAAAAAATCGTGGGCCAAGCTTTGCGACGCATTCCTAGTAGAAAGTGAATGGTTCGTTTCTGGTTATTCACCAAGTGCCAAAGAGTATCTAAGAAATGGAGCACTTTTAGACAACGACCCTGAAATTATTTCTTCTACCGCAACAATCTTGAGGCTTCAAGACGACTTAGGAAGCGCCAAGGATGAGAATCAAGACGGATATGATGGATCATACGTGAATTACTACATGAAAGACAATAAAGATGCTTCGATTGAGAGCTCACGACAACACATAGCCAATTTGATCTCGCATGCATGGAAGAAGCTCAATAGAGAATGTTTGTCGTTGAGTCCATTTCCTCTTGCGTTCATGGAGATTTCTTTGAATATTGCGAGATTTGTTCCGATTTTATATGGTTATGATGAAAATCAAAACCTTCCAACGCTTAAGAAGCTTGTGAAATCGATGTTGTATGAAAGAGCGGAGATATAA

Coding sequence (CDS)

ATGGCCGAAACAAAGATCCAGCAACGCCCTATTAATCCTCCTCAAAACCCCTTTCCAACAACATGGAGCGATCTAGATGACCCATTGGAAGCTTTAAGCTTGATCGATACGGCTCACCGTCTCGGCATCGCCTACCGTTTCCAAGCCGAGGTCGACCTTATTTTACAACGCCATTACGCAGCCTCACTCAACCTCGACGTTGCTTATGCAAACCACGAACTTTATGAAGTCGCTCTTCGCTTTCGACTCCTCCGACAAGGTGGCTACTTTGTGCCGTCGGACATGTTCGAGGCTTTCATGGACGAGGATGGCCATTTCAAGCAGGAGCTTGAGAATGATATCAATGGGGTGATGAGTTTGTTTGAAGCTTCGCAATTTTGCTTGCCAGGCGAAGCTATACTCGAGGAAGCTCGAGTTTTTAGCGCTCGAATCATGAGTGAATACGTCATGAACAATGTTGATTGTAAAAAAGCTAGGCATGTTGCTAGAGCTTTGGCGAATCCTTATCATACGAGCTTCTCTAAGTTCTTGGTGAAGGATTATTTTGGAATGGGGGATTTACCGGACACGAATAGATTGACCCGTGATTTCCAACTTGTCGCCAAAATGGATTTCAACGTTGCCCAGGACATGCGTCGTCGGGAACTTTATCGGTTCACACAATGGTGGACAGACACAGGTTTGGGCAAAGTGTTGGGATTTGCAAGGGACCAACCGCTAAAATGGTACATTTGTTCGTTAGTTTGCCTCACAGATCCATGTTTTTCGGAGGAAAGAGTTGAACTTTCAAAACCCATCTCTTTTATTTATCTTATTGACGACATGTTCGATGTTTACGCCTCGCTCGACCAACTTAAGCTGTTCACAGAAGCTGTTAGAAGATGGGATCTTACGGTGGCTGAAGCACTACCAGACTGTATGAGGATTTGTTTCAAATCACTCTATGAAATGACAAATGAAATTAGTAGTAAAATCCATAAGAAGCATGGTTGGAATCCAATCAACTCCTTAAAAAAATCGTGGGCCAAGCTTTGCGACGCATTCCTAGTAGAAAGTGAATGGTTCGTTTCTGGTTATTCACCAAGTGCCAAAGAGTATCTAAGAAATGGAGCACTTTTAGACAACGACCCTGAAATTATTTCTTCTACCGCAACAATCTTGAGGCTTCAAGACGACTTAGGAAGCGCCAAGGATGAGAATCAAGACGGATATGATGGATCATACGTGAATTACTACATGAAAGACAATAAAGATGCTTCGATTGAGAGCTCACGACAACACATAGCCAATTTGATCTCGCATGCATGGAAGAAGCTCAATAGAGAATGTTTGTCGTTGAGTCCATTTCCTCTTGCGTTCATGGAGATTTCTTTGAATATTGCGAGATTTGTTCCGATTTTATATGGTTATGATGAAAATCAAAACCTTCCAACGCTTAAGAAGCTTGTGAAATCGATGTTGTATGAAAGAGCGGAGATATAA

Protein sequence

MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKDYFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGALLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQNLPTLKKLVKSMLYERAEI
Homology
BLAST of Carg26523 vs. NCBI nr
Match: KAG7012396.1 (hypothetical protein SDJN02_25148, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1010.4 bits (2611), Expect = 5.4e-291
Identity = 493/493 (100.00%), Postives = 493/493 (100.00%), Query Frame = 0

Query: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYA 60
           MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYA
Sbjct: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYA 60

Query: 61  ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120
           ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL
Sbjct: 61  ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120

Query: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKD 180
           FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKD
Sbjct: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKD 180

Query: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240
           YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL
Sbjct: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240

Query: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300
           KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA
Sbjct: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300

Query: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360
           EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS
Sbjct: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360

Query: 361 PSAKEYLRNGALLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDAS 420
           PSAKEYLRNGALLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDAS
Sbjct: 361 PSAKEYLRNGALLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDAS 420

Query: 421 IESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQNLPTLK 480
           IESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQNLPTLK
Sbjct: 421 IESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQNLPTLK 480

Query: 481 KLVKSMLYERAEI 494
           KLVKSMLYERAEI
Sbjct: 481 KLVKSMLYERAEI 493

BLAST of Carg26523 vs. NCBI nr
Match: XP_022954626.1 ((3S,6E)-nerolidol synthase 1 [Cucurbita moschata])

HSP 1 Score: 983.8 bits (2542), Expect = 5.4e-283
Identity = 485/519 (93.45%), Postives = 490/519 (94.41%), Query Frame = 0

Query: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYA 60
           MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAE+DLILQRHYA
Sbjct: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEIDLILQRHYA 60

Query: 61  ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120
           ASLNLDV YANHELYEVALRFRLLRQGGYFVPSD+FEAFMDEDGHFKQELENDINGVMSL
Sbjct: 61  ASLNLDVGYANHELYEVALRFRLLRQGGYFVPSDIFEAFMDEDGHFKQELENDINGVMSL 120

Query: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKD 180
           FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVA+ALANPYHTSFSKFLVKD
Sbjct: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVAKALANPYHTSFSKFLVKD 180

Query: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240
           +FGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL
Sbjct: 181 HFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240

Query: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300
           KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA
Sbjct: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300

Query: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360
           EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS
Sbjct: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360

Query: 361 PSAKEYLRNGA--------------------------LLDNDPEIISSTATILRLQDDLG 420
           PSAKEYLRNGA                          LLDNDPEIISSTATILRLQDDLG
Sbjct: 361 PSAKEYLRNGAVSSGVHVVLVHAFFLLGQRINHETVKLLDNDPEIISSTATILRLQDDLG 420

Query: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFM 480
           SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLIS+AWKKLNRECLSLSPFPLAFM
Sbjct: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISYAWKKLNRECLSLSPFPLAFM 480

Query: 481 EISLNIARFVPILYGYDENQNLPTLKKLVKSMLYERAEI 494
           E SLNIARFVPILYGYDENQNLPTLKKLVKSMLYER EI
Sbjct: 481 ETSLNIARFVPILYGYDENQNLPTLKKLVKSMLYERVEI 519

BLAST of Carg26523 vs. NCBI nr
Match: KAG6573223.1 (hypothetical protein SDJN03_27110, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 963.8 bits (2490), Expect = 5.8e-277
Identity = 476/519 (91.71%), Postives = 485/519 (93.45%), Query Frame = 0

Query: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYA 60
           MAETKIQQR INPPQNPFPTTWSDL+DPLEALSLIDTAHRLGIAYRFQAE+DLILQ HYA
Sbjct: 1   MAETKIQQRLINPPQNPFPTTWSDLNDPLEALSLIDTAHRLGIAYRFQAEIDLILQHHYA 60

Query: 61  ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120
           ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL
Sbjct: 61  ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120

Query: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKD 180
           FEASQ CLPGEAILEEARVFSARIMS YVMNNVDCKKARHVARALANPYHTSFSKFLVKD
Sbjct: 121 FEASQICLPGEAILEEARVFSARIMSVYVMNNVDCKKARHVARALANPYHTSFSKFLVKD 180

Query: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240
           YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRREL++FTQWWTDTGLGKVLGFARDQPL
Sbjct: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELHQFTQWWTDTGLGKVLGFARDQPL 240

Query: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300
           KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYAS DQLKLFTEAVRRWD+TVA
Sbjct: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASFDQLKLFTEAVRRWDVTVA 300

Query: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360
           EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINS KKSW+KLCDAFLVESEWFVSGYS
Sbjct: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSFKKSWSKLCDAFLVESEWFVSGYS 360

Query: 361 PSAKEYLRNGA--------------------------LLDNDPEIISSTATILRLQDDLG 420
           PSAKEYLRNGA                          LLDNDPEIISSTATILRLQDDLG
Sbjct: 361 PSAKEYLRNGAVSSGVHVVLVHAFFLLGQRINHETVKLLDNDPEIISSTATILRLQDDLG 420

Query: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFM 480
           SAKDENQDG+DGSYVNYYMKDNKDASIESSRQHIA+LIS+AWKKLNRECLS SPFPLAFM
Sbjct: 421 SAKDENQDGHDGSYVNYYMKDNKDASIESSRQHIASLISYAWKKLNRECLSSSPFPLAFM 480

Query: 481 EISLNIARFVPILYGYDENQNLPTLKKLVKSMLYERAEI 494
           E SLNIARFVPILYGYDENQNLPTLKKLVKSMLYERAEI
Sbjct: 481 ETSLNIARFVPILYGYDENQNLPTLKKLVKSMLYERAEI 519

BLAST of Carg26523 vs. NCBI nr
Match: XP_023542184.1 ((3S,6E)-nerolidol synthase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 963.8 bits (2490), Expect = 5.8e-277
Identity = 474/519 (91.33%), Postives = 486/519 (93.64%), Query Frame = 0

Query: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYA 60
           MAETKIQQRPINPPQNPFPTTWSDL+DPLEAL+LID AHRLGIAYRFQAE+DLILQRHYA
Sbjct: 1   MAETKIQQRPINPPQNPFPTTWSDLNDPLEALTLIDAAHRLGIAYRFQAEIDLILQRHYA 60

Query: 61  ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120
           ASLNLDV+YANH+L+EVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL
Sbjct: 61  ASLNLDVSYANHDLHEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120

Query: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKD 180
           FEASQFCLPGEAILEEARVFSARIMSEYVM NVDC KARHVARALANPYHTSFSKF+VKD
Sbjct: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMKNVDCNKARHVARALANPYHTSFSKFMVKD 180

Query: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240
           YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL
Sbjct: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240

Query: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300
           KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWD+TVA
Sbjct: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDVTVA 300

Query: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360
           EALPDCMRICFKSLY MTNEISSKIH+KHGWNPINSL+KSWAKLCDAFLVESEWFVSGYS
Sbjct: 301 EALPDCMRICFKSLYVMTNEISSKIHEKHGWNPINSLQKSWAKLCDAFLVESEWFVSGYS 360

Query: 361 PSAKEYLRNGA--------------------------LLDNDPEIISSTATILRLQDDLG 420
           PSAKEYLRNGA                          LLDNDPEIISSTATILRLQDDLG
Sbjct: 361 PSAKEYLRNGAVSSGVHVVLVHAFFLLGQTINHETVKLLDNDPEIISSTATILRLQDDLG 420

Query: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFM 480
           SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLIS+AWKKLNRECLS S FPLAFM
Sbjct: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISYAWKKLNRECLSSSSFPLAFM 480

Query: 481 EISLNIARFVPILYGYDENQNLPTLKKLVKSMLYERAEI 494
           E SLNIARFVPILYGYD+NQNLPTLKKLVKSMLYERAEI
Sbjct: 481 ETSLNIARFVPILYGYDDNQNLPTLKKLVKSMLYERAEI 519

BLAST of Carg26523 vs. NCBI nr
Match: XP_022994384.1 ((3S,6E)-nerolidol synthase 1 [Cucurbita maxima])

HSP 1 Score: 945.3 bits (2442), Expect = 2.1e-271
Identity = 464/519 (89.40%), Postives = 478/519 (92.10%), Query Frame = 0

Query: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYA 60
           MAETKIQQRPINPP+NPFPT WSDL+DPLEALSLIDTAHRL IAYRFQAE+DLILQRHY+
Sbjct: 1   MAETKIQQRPINPPRNPFPTKWSDLNDPLEALSLIDTAHRLDIAYRFQAEIDLILQRHYS 60

Query: 61  ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120
           ASLNLDV YANH+L+EVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL
Sbjct: 61  ASLNLDVGYANHDLHEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120

Query: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKD 180
           FEASQFC+P EAILEEARVFSARIMSEYVMNNVDCKKA HVA+ALANPYHTSFSKF+VKD
Sbjct: 121 FEASQFCMPNEAILEEARVFSARIMSEYVMNNVDCKKANHVAKALANPYHTSFSKFMVKD 180

Query: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240
           YFGMGDLPDTNR T DFQLVAKMDF+VAQDMRRRELYRFTQWW DTGLGKVLGFARDQPL
Sbjct: 181 YFGMGDLPDTNRWTHDFQLVAKMDFSVAQDMRRRELYRFTQWWKDTGLGKVLGFARDQPL 240

Query: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300
           KWYICSLVC TDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA
Sbjct: 241 KWYICSLVCFTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300

Query: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360
           EALPDCMRICFKSLYEMTNEISSKIH+KHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS
Sbjct: 301 EALPDCMRICFKSLYEMTNEISSKIHRKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360

Query: 361 PSAKEYLRNGA--------------------------LLDNDPEIISSTATILRLQDDLG 420
           PSA+EYLRNGA                          LLDNDPEIISSTATILRLQDDLG
Sbjct: 361 PSAEEYLRNGAVSSGVHVVLVHAFFLLGQRINHETVKLLDNDPEIISSTATILRLQDDLG 420

Query: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFM 480
           SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLS SPF LAF+
Sbjct: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLSSSPFSLAFI 480

Query: 481 EISLNIARFVPILYGYDENQNLPTLKKLVKSMLYERAEI 494
           E SLNIARFVPILYGYDENQ LP L+KLVKSMLYER EI
Sbjct: 481 ETSLNIARFVPILYGYDENQKLPMLEKLVKSMLYERVEI 519

BLAST of Carg26523 vs. ExPASy Swiss-Prot
Match: P0CV94 ((3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.4e-140
Identity = 255/489 (52.15%), Postives = 329/489 (67.28%), Query Frame = 0

Query: 27  DPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALRFRLLRQ 86
           D LE L++ID   RLGI Y FQ E+D IL +       + +  A  +L+EVALRFRLLRQ
Sbjct: 36  DALEGLNMIDAVQRLGIDYNFQREIDEILHK------QMSIVSARDDLHEVALRFRLLRQ 95

Query: 87  GGYFVPSDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSARIMS 146
            GYFVP D+F  F D  G FKQ L  DI G+MSL+EASQ    GE IL EA  FS  ++ 
Sbjct: 96  HGYFVPEDVFNNFKDSKGTFKQVLGEDIKGLMSLYEASQLGTEGEDILVEAEKFSGHLL- 155

Query: 147 EYVMNNVDCKKARHVARALANPYHTSFSKFLVKDYFGMGDLPDTNRLTRDFQLVAKMDFN 206
           +  ++++D  + R VA  L NP+H S + F+ +++F       TN      + VAK DFN
Sbjct: 156 KTSLSHLDHHRVRIVANTLRNPHHKSLAPFMARNFFVTSQA--TNSWLNLLKEVAKTDFN 215

Query: 207 VAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERVELSKPI 266
           + + + + E+ + ++WW + GL K L FARDQPLKWYI S+ CLTDP  SEERVEL+KPI
Sbjct: 216 MVRSLHQNEIVQMSKWWKELGLAKELKFARDQPLKWYIWSMACLTDPKLSEERVELTKPI 275

Query: 267 SFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNEISSKIH 326
           SF+YLIDD+FDVY +LD L LFTEAV RW++T  + LPD M+ICFK+LY+MTNE SSK++
Sbjct: 276 SFVYLIDDIFDVYGTLDDLILFTEAVNRWEITAIDHLPDYMKICFKALYDMTNEFSSKVY 335

Query: 327 KKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNG---------------- 386
            KHGWNP+ SLK SWA LC+AFLVE++WF SG  P ++EYL+NG                
Sbjct: 336 LKHGWNPLQSLKISWASLCNAFLVEAKWFASGKLPKSEEYLKNGIVSSGVNVVLVHMFFL 395

Query: 387 ----------ALLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDAS 446
                      LL+  P IISS+A ILRL DDLGSAKDENQDG DGSYV  Y+++++  S
Sbjct: 396 LGQNITRKSVELLNETPAIISSSAAILRLWDDLGSAKDENQDGNDGSYVRCYLEEHEGCS 455

Query: 447 IESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQNLPTLK 490
           IE +R+   N+IS  WKKLNRE LS +PFP +F   SLN+AR +P++Y YD NQ LP+LK
Sbjct: 456 IEEAREKTINMISDEWKKLNRELLSPNPFPASFTLASLNLARMIPLMYSYDGNQCLPSLK 515

BLAST of Carg26523 vs. ExPASy Swiss-Prot
Match: P0CV96 ((3S,6E)-nerolidol synthase 1, chloroplastic OS=Fragaria vesca OX=57918 PE=1 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.5e-137
Identity = 250/489 (51.12%), Postives = 326/489 (66.67%), Query Frame = 0

Query: 27  DPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALRFRLLRQ 86
           D LE L++ID   RLGI Y FQ E+D IL +       + +  A  +LYEVALRFRLLRQ
Sbjct: 97  DALEGLNMIDAVQRLGIDYHFQREIDEILHK------QMGIVSACDDLYEVALRFRLLRQ 156

Query: 87  GGYFVPSDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSARIMS 146
            GYFVP D+F  F D  G FKQ L  DI G+MSL+EASQ    GE  L EA  FS  ++ 
Sbjct: 157 HGYFVPEDVFNNFKDSKGTFKQVLGEDIKGLMSLYEASQLGTEGEDTLVEAEKFSGHLL- 216

Query: 147 EYVMNNVDCKKARHVARALANPYHTSFSKFLVKDYFGMGDLPDTNRLTRDFQLVAKMDFN 206
           +  ++++D  +AR V   L NP+  S + F+ +++F       TN      + VAK DFN
Sbjct: 217 KTSLSHLDRHRARIVGNTLRNPHRKSLASFMARNFFVTSQA--TNSWLNLLKEVAKTDFN 276

Query: 207 VAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERVELSKPI 266
           + + + ++E+ + ++WW + GL K L FARDQPLKWY  S+  LTDP  SEERVEL+KPI
Sbjct: 277 MVRSVHQKEIVQISKWWKELGLVKELKFARDQPLKWYTWSMAGLTDPKLSEERVELTKPI 336

Query: 267 SFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNEISSKIH 326
           SF+YLIDD+FDVY +LD L LFTEAV RW++T  + LPD M+ICFK+LY+MTNE S K++
Sbjct: 337 SFVYLIDDIFDVYGTLDDLILFTEAVNRWEITAIDHLPDYMKICFKALYDMTNEFSCKVY 396

Query: 327 KKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNG---------------- 386
           +KHGWNP+ SLK SWA LC+AFLVE++WF SG  P ++EYL+NG                
Sbjct: 397 QKHGWNPLRSLKISWASLCNAFLVEAKWFASGQLPKSEEYLKNGIVSSGVNVGLVHMFFL 456

Query: 387 ----------ALLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDAS 446
                      LL+  P +ISS+A ILRL DDLGSAKDENQDG DGSYV  Y+++++  S
Sbjct: 457 LGQNITRKSVELLNETPAMISSSAAILRLWDDLGSAKDENQDGNDGSYVRCYLEEHEGCS 516

Query: 447 IESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQNLPTLK 490
           IE +R+   N+IS  WKKLNRE LS +PFP  F   SLN+AR +P++Y YD NQ+LP+LK
Sbjct: 517 IEEAREKTINMISDEWKKLNRELLSPNPFPATFTSASLNLARMIPLMYSYDGNQSLPSLK 576

BLAST of Carg26523 vs. ExPASy Swiss-Prot
Match: P0CV95 ((3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.5e-137
Identity = 251/489 (51.33%), Postives = 325/489 (66.46%), Query Frame = 0

Query: 27  DPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALRFRLLRQ 86
           D LE L++ID   RLGI + FQ E+D IL +       +    A+ +L+EVALRFRLLRQ
Sbjct: 95  DALEGLNMIDAVQRLGIDFHFQREIDEILHK------QMSNVSASDDLHEVALRFRLLRQ 154

Query: 87  GGYFVPSDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSARIMS 146
            GYFVP D+F  F D  G FKQ L  DI G+MSL+EASQ    GE  L EA  FS  ++ 
Sbjct: 155 HGYFVPEDVFNNFKDSKGTFKQVLGEDIKGLMSLYEASQLGTEGEDTLVEAEKFSGHLL- 214

Query: 147 EYVMNNVDCKKARHVARALANPYHTSFSKFLVKDYFGMGDLPDTNRLTRDFQLVAKMDFN 206
           +  ++++D   AR V   L NP+H S + F+ +++F       TN      + VAK DFN
Sbjct: 215 KTSLSHLDHHHARIVGNTLRNPHHKSLASFMARNFFVTTQA--TNSWLNLLKDVAKTDFN 274

Query: 207 VAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERVELSKPI 266
           + + + + E+ + ++WW + GL K L FARDQP KWYI S+ CLTDP  SEERVEL+KPI
Sbjct: 275 MVRSLHQNEIVQISKWWKELGLAKELKFARDQPQKWYIWSMACLTDPKLSEERVELTKPI 334

Query: 267 SFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNEISSKIH 326
           SF+YLIDD+FDVY +LD L LFTEAV RW++T  + LPD M+ICFK+LY+MTNEIS K++
Sbjct: 335 SFVYLIDDIFDVYGTLDDLILFTEAVNRWEITAIDHLPDYMKICFKALYDMTNEISCKVY 394

Query: 327 KKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNG---------------- 386
           +KHGWNP+ SLK SWA LC+AFLVE++WF SG  P +KEYL+NG                
Sbjct: 395 QKHGWNPLQSLKISWASLCNAFLVEAKWFASGQLPKSKEYLKNGIVSSGVNVVLVHMFFI 454

Query: 387 ----------ALLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDAS 446
                      LL+  P +ISS+A ILRL DDLGSAKDENQDG DGSYV  Y+++++  S
Sbjct: 455 LGQNITTKSVELLNETPAMISSSAAILRLWDDLGSAKDENQDGNDGSYVRCYLEEHEGCS 514

Query: 447 IESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQNLPTLK 490
           IE +R+   N+IS  WKKLNRE LS +PFP      SLN+AR +P++Y YD NQ LP+LK
Sbjct: 515 IEEAREKTINMISDEWKKLNRELLSPNPFPATITLASLNLARMIPLMYSYDGNQCLPSLK 574

BLAST of Carg26523 vs. ExPASy Swiss-Prot
Match: B9RXW4 (Probable terpene synthase 13 OS=Ricinus communis OX=3988 GN=TPS13 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 2.8e-125
Identity = 222/491 (45.21%), Postives = 324/491 (65.99%), Query Frame = 0

Query: 30  EALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALRFRLLRQGGY 89
           E L++ID   RLGI + FQ E+D ILQR Y     +   Y +++L+ +ALRFRLLRQGGY
Sbjct: 87  EVLAMIDAIQRLGIDHHFQDEIDEILQRQY----TIPSYYNDNDLHGLALRFRLLRQGGY 146

Query: 90  FVPSDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGE-AILEEARVFSARIMSEY 149
            V + +F+ F D++G+F Q+L +DI G+M L+EASQ  +  E  IL+EA  +S +++S +
Sbjct: 147 NVSAGVFDKFKDKEGNFDQKLSDDIRGLMELYEASQLSIGAEDHILDEAGDYSHQLLSSW 206

Query: 150 VMNNVDCKKARHVARALANPYHTSFSKFLVKDYFGMGDLPDTNRLTRDFQLVAKMDFNVA 209
            M  +D  +AR +   L +P+H + ++F   ++     + +      + Q +AK+DF + 
Sbjct: 207 -MTRLDDSQARIIKNTLDHPHHKNLARFRATNFNRYFHMANIEGWMNELQELAKIDFQMV 266

Query: 210 QDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERVELSKPISF 269
           Q   ++E+++   WW D G+ K L F R+QPLKWYI S+  L+DP  S++R++L+KPISF
Sbjct: 267 QSQNQQEIFQVAGWWKDLGISKELKFVRNQPLKWYIWSMATLSDPSLSQQRIDLTKPISF 326

Query: 270 IYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNEISSKIHKK 329
           IY+IDD+FDV  SLD+L LFTE V+RWD+   E LP  MR CFK+L  +TNEI  K++K+
Sbjct: 327 IYIIDDIFDVQGSLDELTLFTEIVKRWDVEAVEQLPGYMRACFKALDSVTNEIGYKVYKQ 386

Query: 330 HGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGAL---------------- 389
           HGWNP++SL+++WA LC AFLVE+ WF SG+ P+A+EYL+NG +                
Sbjct: 387 HGWNPVHSLRETWASLCKAFLVEARWFASGHLPAAEEYLQNGIVSSGVHVVLVHIFYLLG 446

Query: 390 ----------LDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDASIE 449
                     + N P II+STATILRL DDLG +KDENQDG+DGSYV  Y+K++K + +E
Sbjct: 447 HGVTREGVDFIGNRPAIITSTATILRLWDDLGISKDENQDGHDGSYVECYVKEHKGSLVE 506

Query: 450 SSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQNLPTLKKL 494
            + + +  +IS AWK+LN+ECL  +PF   F +  LN+AR VP++Y YD+N  LP L+  
Sbjct: 507 IATKKVTVMISDAWKQLNQECLHPNPFSPNFTKSCLNLARMVPLMYSYDDNHRLPVLEYY 566

BLAST of Carg26523 vs. ExPASy Swiss-Prot
Match: B9RHX7 (Probable terpene synthase 4 OS=Ricinus communis OX=3988 GN=TPS4 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 2.9e-122
Identity = 226/495 (45.66%), Postives = 316/495 (63.84%), Query Frame = 0

Query: 27  DPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALRFRLLRQ 86
           D  + L +ID   RLGI Y FQ E   +L+  Y     +  A+  H L + ALRFRLLRQ
Sbjct: 73  DHSQNLIMIDALQRLGIDYHFQEETQDVLEGQYN---KIFAAHQQHHLSDAALRFRLLRQ 132

Query: 87  GGYFVP-SDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSARIM 146
            GY+VP SD+F    + +G FKQEL  DI G+M L+EASQ  +  E IL+EA  FSA  +
Sbjct: 133 QGYYVPASDVFSELKNREGKFKQELAADIKGLMELYEASQLSIQEENILDEAGAFSAHFL 192

Query: 147 SEYVMNNVDCKKARHVARALANPYHTSFSKFLVKDYFGMGDLPDTNRLTRDFQLVAKMDF 206
           + +  +  D  + R V+  L +P+H + ++  +K++    +    N   + F  +AK+DF
Sbjct: 193 NCWTPHLCD-HQTRIVSNTLKHPFHRTLARSTIKNFLHFYNFQGENEYIQTFTELAKLDF 252

Query: 207 NVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERVELSKP 266
           N+ Q + R+E+ + + WW + GL   L FARDQP KW +  LV +TDP  S +R+EL+KP
Sbjct: 253 NMIQSIHRQEINQVSNWWNNLGLASELKFARDQPEKWCMWPLVGVTDPSLSWQRIELAKP 312

Query: 267 ISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNEISSKI 326
           +S +YLIDD+FD+  + DQL LFTEAV RW++T  E LP  M+ICF++LY++TN+I+ K+
Sbjct: 313 VSLVYLIDDIFDLGGTPDQLTLFTEAVNRWEITATEDLPYHMKICFRALYDVTNQIAYKV 372

Query: 327 HKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGA-------------- 386
           +KKH +NPI+SLKK+WA+LC+AFL E++WF +G  P A EYL                  
Sbjct: 373 YKKHQYNPIHSLKKAWARLCNAFLEEAKWFAAGKLPKADEYLNTAIVTSGVHLVLVHTFF 432

Query: 387 ------------LLDN-DPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKD 446
                       LL+N DP IISS ATILRL DDLGSA+DENQDGYDGSY+  YMKD   
Sbjct: 433 LMGDGITDQTINLLNNDDPGIISSVATILRLWDDLGSAQDENQDGYDGSYIECYMKDFPG 492

Query: 447 ASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQNLPT 494
            S+  +R H+ ++IS  WKKLN+ CLS +PF  +F+  +LN AR VP++Y +D N NLP 
Sbjct: 493 TSVRDARNHVISMISDTWKKLNQHCLSPNPFSGSFIRATLNGARMVPLMYDFDSNHNLPI 552

BLAST of Carg26523 vs. ExPASy TrEMBL
Match: A0A6J1GRE0 ((3S,6E)-nerolidol synthase 1 OS=Cucurbita moschata OX=3662 GN=LOC111456834 PE=4 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 2.6e-283
Identity = 485/519 (93.45%), Postives = 490/519 (94.41%), Query Frame = 0

Query: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYA 60
           MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAE+DLILQRHYA
Sbjct: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEIDLILQRHYA 60

Query: 61  ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120
           ASLNLDV YANHELYEVALRFRLLRQGGYFVPSD+FEAFMDEDGHFKQELENDINGVMSL
Sbjct: 61  ASLNLDVGYANHELYEVALRFRLLRQGGYFVPSDIFEAFMDEDGHFKQELENDINGVMSL 120

Query: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKD 180
           FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVA+ALANPYHTSFSKFLVKD
Sbjct: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVAKALANPYHTSFSKFLVKD 180

Query: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240
           +FGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL
Sbjct: 181 HFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240

Query: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300
           KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA
Sbjct: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300

Query: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360
           EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS
Sbjct: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360

Query: 361 PSAKEYLRNGA--------------------------LLDNDPEIISSTATILRLQDDLG 420
           PSAKEYLRNGA                          LLDNDPEIISSTATILRLQDDLG
Sbjct: 361 PSAKEYLRNGAVSSGVHVVLVHAFFLLGQRINHETVKLLDNDPEIISSTATILRLQDDLG 420

Query: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFM 480
           SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLIS+AWKKLNRECLSLSPFPLAFM
Sbjct: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISYAWKKLNRECLSLSPFPLAFM 480

Query: 481 EISLNIARFVPILYGYDENQNLPTLKKLVKSMLYERAEI 494
           E SLNIARFVPILYGYDENQNLPTLKKLVKSMLYER EI
Sbjct: 481 ETSLNIARFVPILYGYDENQNLPTLKKLVKSMLYERVEI 519

BLAST of Carg26523 vs. ExPASy TrEMBL
Match: A0A6J1K2Q2 ((3S,6E)-nerolidol synthase 1 OS=Cucurbita maxima OX=3661 GN=LOC111490118 PE=4 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 1.0e-271
Identity = 464/519 (89.40%), Postives = 478/519 (92.10%), Query Frame = 0

Query: 1   MAETKIQQRPINPPQNPFPTTWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYA 60
           MAETKIQQRPINPP+NPFPT WSDL+DPLEALSLIDTAHRL IAYRFQAE+DLILQRHY+
Sbjct: 1   MAETKIQQRPINPPRNPFPTKWSDLNDPLEALSLIDTAHRLDIAYRFQAEIDLILQRHYS 60

Query: 61  ASLNLDVAYANHELYEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120
           ASLNLDV YANH+L+EVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL
Sbjct: 61  ASLNLDVGYANHDLHEVALRFRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSL 120

Query: 121 FEASQFCLPGEAILEEARVFSARIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKD 180
           FEASQFC+P EAILEEARVFSARIMSEYVMNNVDCKKA HVA+ALANPYHTSFSKF+VKD
Sbjct: 121 FEASQFCMPNEAILEEARVFSARIMSEYVMNNVDCKKANHVAKALANPYHTSFSKFMVKD 180

Query: 181 YFGMGDLPDTNRLTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPL 240
           YFGMGDLPDTNR T DFQLVAKMDF+VAQDMRRRELYRFTQWW DTGLGKVLGFARDQPL
Sbjct: 181 YFGMGDLPDTNRWTHDFQLVAKMDFSVAQDMRRRELYRFTQWWKDTGLGKVLGFARDQPL 240

Query: 241 KWYICSLVCLTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300
           KWYICSLVC TDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA
Sbjct: 241 KWYICSLVCFTDPCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVA 300

Query: 301 EALPDCMRICFKSLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360
           EALPDCMRICFKSLYEMTNEISSKIH+KHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS
Sbjct: 301 EALPDCMRICFKSLYEMTNEISSKIHRKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYS 360

Query: 361 PSAKEYLRNGA--------------------------LLDNDPEIISSTATILRLQDDLG 420
           PSA+EYLRNGA                          LLDNDPEIISSTATILRLQDDLG
Sbjct: 361 PSAEEYLRNGAVSSGVHVVLVHAFFLLGQRINHETVKLLDNDPEIISSTATILRLQDDLG 420

Query: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFM 480
           SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLS SPF LAF+
Sbjct: 421 SAKDENQDGYDGSYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLSSSPFSLAFI 480

Query: 481 EISLNIARFVPILYGYDENQNLPTLKKLVKSMLYERAEI 494
           E SLNIARFVPILYGYDENQ LP L+KLVKSMLYER EI
Sbjct: 481 ETSLNIARFVPILYGYDENQKLPMLEKLVKSMLYERVEI 519

BLAST of Carg26523 vs. ExPASy TrEMBL
Match: A0A5A7UTP7 ((3S,6E)-nerolidol synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G00790 PE=4 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 3.0e-194
Identity = 348/546 (63.74%), Postives = 414/546 (75.82%), Query Frame = 0

Query: 1   MAETKIQQ--RPINPPQNPFPTTWS------------------DLDDPLEALSLIDTAHR 60
           MAET  +Q    +NPPQN   +  S                   LDDPLE+L+LID   R
Sbjct: 1   MAETNNEQCLVQLNPPQNSVLSFNSSSFQSLSNNSHEVELMSCSLDDPLESLNLIDAVQR 60

Query: 61  LGIAYRFQAEVDLILQRHYAASLNLDVAYAN-HELYEVALRFRLLRQGGYFVPSDMFEAF 120
           LG+ YRFQ E+D IL+ HYA  ++ D    N ++L+EVALRFRLLRQ GYFV SD+F  F
Sbjct: 61  LGLEYRFQTEIDAILEHHYAIFVDHDGDCMNDNDLHEVALRFRLLRQRGYFVSSDVFNIF 120

Query: 121 MDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSARIMSEYVMN-NVDCKKA 180
           M+EDGHFKQ LE+DI G+MSL+EASQ CLPGE  L EA+ FS++I+ EY+ N N+D K+A
Sbjct: 121 MNEDGHFKQNLEHDIKGLMSLYEASQLCLPGENKLNEAQNFSSKILREYITNDNIDNKEA 180

Query: 181 RHVARALANPYHTSFSKFLVKDYFGMGD----LPDTNRLTRDFQLVAKMDFNVAQDMRRR 240
            HVAR LANPYHTSFSKF+VKDYFG+GD        N+    FQ VAKMDFN+AQ+M ++
Sbjct: 181 NHVARTLANPYHTSFSKFMVKDYFGVGDDELLHATNNKWVHAFQQVAKMDFNIAQNMHQQ 240

Query: 241 ELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERVELSKPISFIYLIDD 300
           EL +FT+WW +TGLGK L FARDQPLKWYICSLVCL DPCFS+ER+ELSK +SF+YLIDD
Sbjct: 241 ELVQFTRWWKETGLGKALPFARDQPLKWYICSLVCLIDPCFSDERIELSKAVSFVYLIDD 300

Query: 301 MFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNEISSKIHKKHGWNPI 360
           MFD+Y SLDQL LFTEAVRRWD+ + EALP+CMRICFKSLY+MTNEISSKI+KKHGWNPI
Sbjct: 301 MFDIYGSLDQLTLFTEAVRRWDIAMTEALPECMRICFKSLYDMTNEISSKIYKKHGWNPI 360

Query: 361 NSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGA----------------------- 420
           NSL+KSWAKLCDAFLVE  WF  G SPSA+EYL+NG                        
Sbjct: 361 NSLQKSWAKLCDAFLVEGRWFDCGESPSAEEYLKNGVVSTGVHVVLVHAFFFLGQSITNQ 420

Query: 421 ---LLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDASIESSRQHI 480
              LLDNDP IISS ATILRL DDLG+AKDENQ+GYDGSYVNYYM  N+D S++++R+H+
Sbjct: 421 TVHLLDNDPLIISSPATILRLHDDLGTAKDENQEGYDGSYVNYYMIHNQDVSVDTTRKHM 480

Query: 481 ANLISHAWKKLNRECLSL-SPFPLAFMEISLNIARFVPILYGYDENQNLPTLKKLVKSML 494
           ANLIS++WK LN+ECLS  SPFP AF++ SL+IARFVPILYGYD+NQ LPTL+KLVKSML
Sbjct: 481 ANLISNSWKNLNKECLSFNSPFPAAFIQASLSIARFVPILYGYDKNQRLPTLEKLVKSML 540

BLAST of Carg26523 vs. ExPASy TrEMBL
Match: A0A1S3B401 ((3S,6E)-nerolidol synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103485753 PE=4 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 3.0e-194
Identity = 348/546 (63.74%), Postives = 414/546 (75.82%), Query Frame = 0

Query: 1   MAETKIQQ--RPINPPQNPFPTTWS------------------DLDDPLEALSLIDTAHR 60
           MAET  +Q    +NPPQN   +  S                   LDDPLE+L+LID   R
Sbjct: 1   MAETNNEQCLVQLNPPQNSVLSFNSSSFQSLSNNSHEVELMSCSLDDPLESLNLIDAVQR 60

Query: 61  LGIAYRFQAEVDLILQRHYAASLNLDVAYAN-HELYEVALRFRLLRQGGYFVPSDMFEAF 120
           LG+ YRFQ E+D IL+ HYA  ++ D    N ++L+EVALRFRLLRQ GYFV SD+F  F
Sbjct: 61  LGLEYRFQTEIDAILEHHYAIFVDHDGDCMNDNDLHEVALRFRLLRQRGYFVSSDVFNIF 120

Query: 121 MDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSARIMSEYVMN-NVDCKKA 180
           M+EDGHFKQ LE+DI G+MSL+EASQ CLPGE  L EA+ FS++I+ EY+ N N+D K+A
Sbjct: 121 MNEDGHFKQNLEHDIKGLMSLYEASQLCLPGENKLNEAQNFSSKILREYITNDNIDNKEA 180

Query: 181 RHVARALANPYHTSFSKFLVKDYFGMGD----LPDTNRLTRDFQLVAKMDFNVAQDMRRR 240
            HVAR LANPYHTSFSKF+VKDYFG+GD        N+    FQ VAKMDFN+AQ+M ++
Sbjct: 181 NHVARTLANPYHTSFSKFMVKDYFGVGDDELLHATNNKWVHAFQQVAKMDFNIAQNMHQQ 240

Query: 241 ELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERVELSKPISFIYLIDD 300
           EL +FT+WW +TGLGK L FARDQPLKWYICSLVCL DPCFS+ER+ELSK +SF+YLIDD
Sbjct: 241 ELVQFTRWWKETGLGKALPFARDQPLKWYICSLVCLIDPCFSDERIELSKAVSFVYLIDD 300

Query: 301 MFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNEISSKIHKKHGWNPI 360
           MFD+Y SLDQL LFTEAVRRWD+ + EALP+CMRICFKSLY+MTNEISSKI+KKHGWNPI
Sbjct: 301 MFDIYGSLDQLTLFTEAVRRWDIAMTEALPECMRICFKSLYDMTNEISSKIYKKHGWNPI 360

Query: 361 NSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGA----------------------- 420
           NSL+KSWAKLCDAFLVE  WF  G SPSA+EYL+NG                        
Sbjct: 361 NSLQKSWAKLCDAFLVEGRWFDCGESPSAEEYLKNGVVSTGVHVVLVHAFFFLGQSITNQ 420

Query: 421 ---LLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDASIESSRQHI 480
              LLDNDP IISS ATILRL DDLG+AKDENQ+GYDGSYVNYYM  N+D S++++R+H+
Sbjct: 421 TVHLLDNDPLIISSPATILRLHDDLGTAKDENQEGYDGSYVNYYMIHNQDVSVDTTRKHM 480

Query: 481 ANLISHAWKKLNRECLSL-SPFPLAFMEISLNIARFVPILYGYDENQNLPTLKKLVKSML 494
           ANLIS++WK LN+ECLS  SPFP AF++ SL+IARFVPILYGYD+NQ LPTL+KLVKSML
Sbjct: 481 ANLISNSWKNLNKECLSFNSPFPAAFIQASLSIARFVPILYGYDKNQRLPTLEKLVKSML 540

BLAST of Carg26523 vs. ExPASy TrEMBL
Match: A0A5D3BHX5 ((3S,6E)-nerolidol synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006280 PE=4 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.9e-188
Identity = 337/546 (61.72%), Postives = 409/546 (74.91%), Query Frame = 0

Query: 1   MAETKIQQ--RPINPPQNPFPTTWS------------------DLDDPLEALSLIDTAHR 60
           MAET  +Q    +NPPQN   +  S                   LDDPLE+L+LID   R
Sbjct: 1   MAETNNEQCLVQLNPPQNSVLSFNSSSFQSLSNNSHEVELMSCSLDDPLESLNLIDAVQR 60

Query: 61  LGIAYRFQAEVDLILQRHYAASLNLDVAYAN-HELYEVALRFRLLRQGGYFVPSDMFEAF 120
           LG+ YRFQ E+D IL+ HYA  ++ D    N ++L+EVALRFRLLRQ GYFV SD+F  F
Sbjct: 61  LGLEYRFQTEIDAILEHHYAIFVDHDGDCMNDNDLHEVALRFRLLRQRGYFVSSDVFNIF 120

Query: 121 MDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSARIMSEYVMN-NVDCKKA 180
           M+EDGHFKQ LE+DI G+MSL+EASQ CLPGE  L EA+ FS++I+ EY+ N N+D K+A
Sbjct: 121 MNEDGHFKQNLEHDIKGLMSLYEASQLCLPGENKLNEAQNFSSKILREYITNDNIDNKEA 180

Query: 181 RHVARALANPYHTSFSKFLVKDYFGMGD----LPDTNRLTRDFQLVAKMDFNVAQDMRRR 240
            HVAR LANPYHTSFSKF+VKDYFG+GD        N+    FQ VAKMDFN+AQ+M ++
Sbjct: 181 NHVARTLANPYHTSFSKFMVKDYFGVGDDELLHATNNKWVHAFQQVAKMDFNIAQNMHQQ 240

Query: 241 ELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERVELSKPISFIYLIDD 300
           EL +FT+WW +TGLGK L FARDQPLKWYICSLVCL DPCFS+ER+ELSK +SF+YLIDD
Sbjct: 241 ELVQFTRWWKETGLGKALPFARDQPLKWYICSLVCLIDPCFSDERIELSKAVSFVYLIDD 300

Query: 301 MFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNEISSKIHKKHGWNPI 360
           MFD+Y SLDQL LFTEAVRRWD+ + EALP+CMRICFKSLY+MTNEISSKI+KKHGWNPI
Sbjct: 301 MFDIYGSLDQLTLFTEAVRRWDIAMTEALPECMRICFKSLYDMTNEISSKIYKKHGWNPI 360

Query: 361 NSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGA----------------------- 420
           NSL+KSWAKLCDAFLVE  WF  G SPSA+EYL+NG                        
Sbjct: 361 NSLQKSWAKLCDAFLVEGRWFDCGESPSAEEYLKNGVVSTGVHVVLVHAFFFLGQSITNQ 420

Query: 421 ---LLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMKDNKDASIESSRQHI 480
              LLDNDP IISS ATILRL DDLG+AKDENQ+GYDGSY++YY+K+N + S++S+RQ +
Sbjct: 421 TVHLLDNDPLIISSPATILRLHDDLGTAKDENQEGYDGSYIDYYLKENPEISVDSARQRV 480

Query: 481 ANLISHAWKKLNRECLS-LSPFPLAFMEISLNIARFVPILYGYDENQNLPTLKKLVKSML 494
            ++IS AWK+LN+E LS  +PFP  F++ S N+ARFVP+LYGYDEN NLPTL+KL+K +L
Sbjct: 481 IHMISDAWKRLNQESLSPNNPFPETFIQASQNMARFVPVLYGYDENHNLPTLEKLMKFVL 540

BLAST of Carg26523 vs. TAIR 10
Match: AT1G61680.1 (terpene synthase 14 )

HSP 1 Score: 387.5 bits (994), Expect = 1.6e-107
Identity = 199/483 (41.20%), Postives = 297/483 (61.49%), Query Frame = 0

Query: 23  SDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALRFR 82
           +++D P E L +ID    LGI   F+ E++  L   Y   L       N +L+E+ALRFR
Sbjct: 85  ANVDVPSENLEMIDVIQSLGIDLHFRQEIEQTLHMIYKEGLQF-----NGDLHEIALRFR 144

Query: 83  LLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSA 142
           LLRQ G++V   +F+  +D+ G FK  ++ND+ G+  LFEAS+  + GE  L+ AR F+ 
Sbjct: 145 LLRQEGHYVQEIIFKNILDKKGGFKDVVKNDVKGLTELFEASELRVEGEETLDGAREFTY 204

Query: 143 RIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKDYFGMGDL--PDTNRLTRDFQLV 202
             ++E + +  +  + + + ++LA P H +      K +  M  +   +     +    V
Sbjct: 205 SRLNE-LCSGRESHQKQEIMKSLAQPRHKTVRGLTSKRFTSMIKIAGQEDPEWLQSLLRV 264

Query: 203 AKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERV 262
           A++D    + + + E+ +  +WWT+ GL K +  AR QPLKW+  S+  L DP  +E+R+
Sbjct: 265 AEIDSIRLKSLTQGEMSQTFKWWTELGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRL 324

Query: 263 ELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNE 322
           +L+KPIS +Y+IDD+FDVY  L++L +FT  V RWD    + LP  MR+CF++L  +T E
Sbjct: 325 DLTKPISLVYVIDDIFDVYGELEELTIFTRVVERWDHKGLKTLPKYMRVCFEALDMITTE 384

Query: 323 ISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGA--------- 382
           IS KI+K HGWNP  +L++SWA LC AFLVE++WF SGY P+ +EY++NG          
Sbjct: 385 ISMKIYKSHGWNPTYALRQSWASLCKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVM 444

Query: 383 -----------------LLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMK 442
                            L++++P I+SS ATILRL DDLGSAKDENQDG DGSYV  Y+ 
Sbjct: 445 LHAYILLGEELTKEKVELIESNPGIVSSAATILRLWDDLGSAKDENQDGTDGSYVECYLN 504

Query: 443 DNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQ 478
           + K ++++ +R H+A  IS AWK+LNRECL+  PF  +F +  LNIAR VP++Y YD++Q
Sbjct: 505 EYKGSTVDEARTHVAQKISRAWKRLNRECLNPCPFSRSFSKACLNIARTVPLMYSYDDDQ 561

BLAST of Carg26523 vs. TAIR 10
Match: AT1G61680.2 (terpene synthase 14 )

HSP 1 Score: 376.7 bits (966), Expect = 2.8e-104
Identity = 197/483 (40.79%), Postives = 293/483 (60.66%), Query Frame = 0

Query: 23  SDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALRFR 82
           +++D P E L +ID    LGI   F+ E++  L   Y   L       N +L+E+ALRFR
Sbjct: 85  ANVDVPSENLEMIDVIQSLGIDLHFRQEIEQTLHMIYKEGLQF-----NGDLHEIALRFR 144

Query: 83  LLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVFSA 142
           LLRQ G++V         ++ G FK  ++ND+ G+  LFEAS+  + GE  L+ AR F+ 
Sbjct: 145 LLRQEGHYVQE-------NKKGGFKDVVKNDVKGLTELFEASELRVEGEETLDGAREFTY 204

Query: 143 RIMSEYVMNNVDCKKARHVARALANPYHTSFSKFLVKDYFGMGDL--PDTNRLTRDFQLV 202
             ++E + +  +  + + + ++LA P H +      K +  M  +   +     +    V
Sbjct: 205 SRLNE-LCSGRESHQKQEIMKSLAQPRHKTVRGLTSKRFTSMIKIAGQEDPEWLQSLLRV 264

Query: 203 AKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERV 262
           A++D    + + + E+ +  +WWT+ GL K +  AR QPLKW+  S+  L DP  +E+R+
Sbjct: 265 AEIDSIRLKSLTQGEMSQTFKWWTELGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRL 324

Query: 263 ELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNE 322
           +L+KPIS +Y+IDD+FDVY  L++L +FT  V RWD    + LP  MR+CF++L  +T E
Sbjct: 325 DLTKPISLVYVIDDIFDVYGELEELTIFTRVVERWDHKGLKTLPKYMRVCFEALDMITTE 384

Query: 323 ISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGA--------- 382
           IS KI+K HGWNP  +L++SWA LC AFLVE++WF SGY P+ +EY++NG          
Sbjct: 385 ISMKIYKSHGWNPTYALRQSWASLCKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVM 444

Query: 383 -----------------LLDNDPEIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYMK 442
                            L++++P I+SS ATILRL DDLGSAKDENQDG DGSYV  Y+ 
Sbjct: 445 LHAYILLGEELTKEKVELIESNPGIVSSAATILRLWDDLGSAKDENQDGTDGSYVECYLN 504

Query: 443 DNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDENQ 478
           + K ++++ +R H+A  IS AWK+LNRECL+  PF  +F +  LNIAR VP++Y YD++Q
Sbjct: 505 EYKGSTVDEARTHVAQKISRAWKRLNRECLNPCPFSRSFSKACLNIARTVPLMYSYDDDQ 554

BLAST of Carg26523 vs. TAIR 10
Match: AT4G16730.1 (terpene synthase 02 )

HSP 1 Score: 267.7 bits (683), Expect = 1.9e-71
Identity = 158/499 (31.66%), Postives = 263/499 (52.71%), Query Frame = 0

Query: 21  TWSDLDDPLEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALR 80
           T ++++  +E L +ID+  RLGI+Y ++ E+  IL++ +    + ++     +L+  +L 
Sbjct: 34  TLNEIEGSIEQLEMIDSLQRLGISYHYKHEIHDILRKIH--DQHGEIERETQDLHATSLE 93

Query: 81  FRLLRQGGYFVPSDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGEAILEEARVF 140
           F LLRQ G+ V  D F+ F+ E G F++ L +DI G++SL+EAS F +  E  L+E R++
Sbjct: 94  FILLRQHGFDVSQDAFDVFISETGEFRKTLHSDIKGLLSLYEASYFSMDSEFKLKETRIY 153

Query: 141 SARIMSEYV--MNNVDCKK-----ARHVARALANPYHTSFSKFLVKDYFGMGDLP-DTNR 200
           + + +SE+V   +   C++        V RAL  PYH S  +   + Y  + +   + N 
Sbjct: 154 ANKRLSEFVAESSKTICREDETYILEMVKRALETPYHWSIRRLEARWYINVYEKKHEMNP 213

Query: 201 LTRDFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTD 260
           L  +F   A +DFN+ Q   + EL   + WW  TGL K L F RD+  + Y  ++    +
Sbjct: 214 LLLEF---AAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRITESYFWTIGIFYE 273

Query: 261 PCFSEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFK 320
           P F   R  L+K    I ++DD++D+Y +L++L+LFT  V +WD+   E LP+ MR+CF 
Sbjct: 274 PEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKWDVNHVERLPNYMRMCFL 333

Query: 321 SLYEMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGAL 380
            LY   N+I   + +  G N I  LK+ W  L   FL ES+W+ +G+ PS +EY++NG +
Sbjct: 334 FLYNEINQIGYDVLRDKGLNVIPYLKQVWTDLFKTFLTESKWYKTGHKPSFEEYMQNGVI 393

Query: 381 LDNDP--------------------------EIISSTATILRLQDDLGSAKDENQDGYDG 440
             + P                           ++ S ATILRL +DL ++ +E   G   
Sbjct: 394 SSSVPTILLHLFSVLSDHISDQTLTDDSKNHSVVRSCATILRLANDLATSTEEMARGDSP 453

Query: 441 SYVNYYMKDNKDASIESSRQHIANLISHAWKKLNRECLS--LSPFPLAFMEISLNIARFV 484
             V  YM + + AS E +R+H+ ++IS +W  +N +  +   S  P  F+  + N+ R V
Sbjct: 454 KSVQCYMYETR-ASEEEARRHMQSMISDSWDIINSDLKTAHTSSLPRGFLAAAANLNRVV 513

BLAST of Carg26523 vs. TAIR 10
Match: AT4G16740.1 (terpene synthase 03 )

HSP 1 Score: 250.8 bits (639), Expect = 2.3e-66
Identity = 160/491 (32.59%), Postives = 252/491 (51.32%), Query Frame = 0

Query: 29  LEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANH-------ELYEVALRF 88
           LE L LIDT  RLG++Y F+ E+   L   +  ++    A+ N        +LY  AL F
Sbjct: 75  LEQLELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVR---AHKNRIDRNRWGDLYATALEF 134

Query: 89  RLLRQGGYFVPSDMFEAFMDEDGHFKQELEN-DINGVMSLFEASQFCLPGEAILEEARVF 148
           RLLRQ G+ +  D+F      DG+   +L++ DI G++SL+EAS      +  L+E+  +
Sbjct: 135 RLLRQHGFSIAQDVF------DGNIGVDLDDKDIKGILSLYEASYLSTRIDTKLKESIYY 194

Query: 149 SARIMSEYVMNNVDCKKA----RHVARALANPYHTSFSKFLVKDYFGM-GDLPDTNRLTR 208
           + + + ++V  N +  K+    R V  AL  PYH    +   + Y  + G+  D N +  
Sbjct: 195 TTKRLRKFVEVNKNETKSYTLRRMVIHALEMPYHRRVGRLEARWYIEVYGERHDMNPILL 254

Query: 209 DFQLVAKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCF 268
           +   +AK+DFN  Q + + EL   + WW+ TGL K L F RD+  + Y  S+  + +P F
Sbjct: 255 E---LAKLDFNFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFSSVGVMYEPEF 314

Query: 269 SEERVELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLY 328
           +  R  L+K    I  IDD++D+Y +L++L+LFT  V +WD+   E LP+ M++CF  L 
Sbjct: 315 AYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKWDVNRLEELPNYMKLCFLCLV 374

Query: 329 EMTNEISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGALLDN 388
              N+I   + +  G+N I  LK+SWA +C  FL E++W+ SGY P+ +EY++NG +  +
Sbjct: 375 NEINQIGYFVLRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKPNFEEYMQNGWISSS 434

Query: 389 DP-----------------------EIISSTATILRLQDDLGSAKDENQDGYDGSYVNYY 448
            P                        ++ S+ATILRL +DL ++ +E   G     V  +
Sbjct: 435 VPTILLHLFCLLSDQTLDILGSYNHSVVRSSATILRLANDLATSSEELARGDTMKSVQCH 494

Query: 449 MKDNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPLAFMEISLNIARFVPILYGYDE 484
           M +   AS   SR +I  +I  AW  LN E  S       F+E + N+ R    +Y Y +
Sbjct: 495 MHET-GASEAESRAYIQGIIGVAWDDLNMEKKSCR-LHQGFLEAAANLGRVAQCVYQYGD 551

BLAST of Carg26523 vs. TAIR 10
Match: AT2G24210.1 (terpene synthase 10 )

HSP 1 Score: 239.6 bits (610), Expect = 5.4e-63
Identity = 149/496 (30.04%), Postives = 255/496 (51.41%), Query Frame = 0

Query: 29  LEALSLIDTAHRLGIAYRFQAEVDLILQRHYAASLNLDVAYANHELYEVALRFRLLRQGG 88
           L+ L  ID   +LG++Y F+AE+D IL   Y             +L+  AL FRL RQ G
Sbjct: 96  LDQLEFIDDLQKLGVSYHFEAEIDNILTSSYKKD---RTNIQESDLHATALEFRLFRQHG 155

Query: 89  YFVPSDMFEAFMDEDGHFKQELENDINGVMSLFEASQFCLPGEAILE-EARVFSARIMSE 148
           + V  D+F+ FM+  G F ++   DI G++SL+EAS      +  L+   R F+ + + +
Sbjct: 156 FNVSEDVFDVFMENCGKFDRD---DIYGLISLYEASYLSTKLDKNLQIFIRPFATQQLRD 215

Query: 149 YV--MNNVD---CKKARHVARALANPYHTSFSKFLVKDYFGMGDLPDTNRLTRDFQLV-- 208
           +V   +N D   C     V +AL  PY+    +   + Y    D+    +  ++  +V  
Sbjct: 216 FVDTHSNEDFGSCDMVEIVVQALDMPYYWQMRRLSTRWYI---DVYGKRQNYKNLVVVEF 275

Query: 209 AKMDFNVAQDMRRRELYRFTQWWTDTGLGKVLGFARDQPLKWYICSLVCLTDPCFSEERV 268
           AK+DFN+ Q + + EL   + WW +TGLGK L FARD+ ++ Y  ++  + +P +   R 
Sbjct: 276 AKIDFNIVQAIHQEELKNVSSWWMETGLGKQLYFARDRIVENYFWTIGQIQEPQYGYVRQ 335

Query: 269 ELSKPISFIYLIDDMFDVYASLDQLKLFTEAVRRWDLTVAEALPDCMRICFKSLYEMTNE 328
            ++K  + +  IDD++D+Y +L++L+LFT A   WD+   + LP+ MR+CF  +Y   N 
Sbjct: 336 TMTKINALLTTIDDIYDIYGTLEELQLFTVAFENWDINRLDELPEYMRLCFLVIYNEVNS 395

Query: 329 ISSKIHKKHGWNPINSLKKSWAKLCDAFLVESEWFVSGYSPSAKEYLRNGALLDNDP--- 388
           I+ +I +    N I  LKKSW  +  A+LVE++W+ SG+ P+ +EY++N  +  + P   
Sbjct: 396 IACEILRTKNINVIPFLKKSWTDVSKAYLVEAKWYKSGHKPNLEEYMQNARISISSPTIF 455

Query: 389 ------------------------EIISSTATILRLQDDLGSAKDENQDGYDGSYVNYYM 448
                                    ++  ++++ RL +DL ++ DE   G     +  YM
Sbjct: 456 VHFYCVFSDQLSIQVLETLSQHQQNVVRCSSSVFRLANDLVTSPDELARGDVCKSIQCYM 515

Query: 449 KDNKDASIESSRQHIANLISHAWKKLNRECLSLSPFPL--AFMEISLNIARFVPILYGYD 488
            +   AS + +R H+  +I+  W ++N E ++ S   L   FME  +N+AR    +Y Y 
Sbjct: 516 SET-GASEDKARSHVRQMINDLWDEMNYEKMAHSSSILHHDFMETVINLARMSQCMYQYG 575

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012396.15.4e-291100.00hypothetical protein SDJN02_25148, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022954626.15.4e-28393.45(3S,6E)-nerolidol synthase 1 [Cucurbita moschata][more]
KAG6573223.15.8e-27791.71hypothetical protein SDJN03_27110, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023542184.15.8e-27791.33(3S,6E)-nerolidol synthase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022994384.12.1e-27189.40(3S,6E)-nerolidol synthase 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P0CV941.4e-14052.15(3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa OX=3747 PE=1 SV=1[more]
P0CV962.5e-13751.12(3S,6E)-nerolidol synthase 1, chloroplastic OS=Fragaria vesca OX=57918 PE=1 SV=1[more]
P0CV952.5e-13751.33(3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa O... [more]
B9RXW42.8e-12545.21Probable terpene synthase 13 OS=Ricinus communis OX=3988 GN=TPS13 PE=3 SV=1[more]
B9RHX72.9e-12245.66Probable terpene synthase 4 OS=Ricinus communis OX=3988 GN=TPS4 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GRE02.6e-28393.45(3S,6E)-nerolidol synthase 1 OS=Cucurbita moschata OX=3662 GN=LOC111456834 PE=4 ... [more]
A0A6J1K2Q21.0e-27189.40(3S,6E)-nerolidol synthase 1 OS=Cucurbita maxima OX=3661 GN=LOC111490118 PE=4 SV... [more]
A0A5A7UTP73.0e-19463.74(3S,6E)-nerolidol synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A1S3B4013.0e-19463.74(3S,6E)-nerolidol synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103485753 PE=4 S... [more]
A0A5D3BHX51.9e-18861.72(3S,6E)-nerolidol synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT1G61680.11.6e-10741.20terpene synthase 14 [more]
AT1G61680.22.8e-10440.79terpene synthase 14 [more]
AT4G16730.11.9e-7131.66terpene synthase 02 [more]
AT4G16740.12.3e-6632.59terpene synthase 03 [more]
AT2G24210.15.4e-6330.04terpene synthase 10 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 194..371
e-value: 6.5E-113
score: 379.8
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 373..491
e-value: 2.7E-34
score: 120.6
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 201..472
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 26..191
e-value: 6.5E-113
score: 379.8
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 373..438
e-value: 1.4E-11
score: 44.3
coord: 201..371
e-value: 2.7E-53
score: 181.0
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 27..165
e-value: 1.1E-37
score: 129.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR31225:SF148(3S,6E)-NEROLIDOL SYNTHASE 2, CHLOROPLASTIC/MITOCHONDRIAL-LIKEcoord: 26..371
coord: 372..488
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 26..371
coord: 372..488
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 26..182

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26523-RACarg26523-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity