Carg26514 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26514
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	tRNA (Guanine(9)-N1)-methyltransferase
Location	Carg_Chr13: 4926569 .. 4930505 (+)
RNA-Seq Expression	Carg26514
Synteny	Carg26514

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCCAGAGAAGAGGACCAGAGCGCCAAAATGGATCCTCCTTGTTCCCCTTCCGGCTGCAATCCTCCAGAGCCCCCCCTCTCCAAGAATGCAAGGAAGAAGCTACTGAAACAACAGCGTTACGAAACGAAGAAAGCGGAGAAGAAAGCTCAGGAAAAAGAGCAGAAGAAGAAAGATATTGAGCGCAAACGGAAGGAATGGGAGGAGACACTGGCGAGTGTTACGGAGGAGGAGAGAGCCAAGCTGATCGAGTCTAGGAAAAGCCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGAGAAGGAGAAGAAGATTGAGCGGCTCAACAGGGCTAAAGATTGTGGTCAGAAGATTGTCATCGACCTTGAATTTTCTGACCTCATGACCCCCTCGGAGATTCACAGTCTTGTTCAGCAGGTTTGTTCTCTTGTTACTCACTTCATTTGGTTGATTAACTATTATTCGTGGTGGTGCGAATACTGGATTGTTTTCTTATATTTTAGAGGTTTGGTAAATGTTTCTAGTAGATGGAATGTAGAGCATCTTAGAAATGAAATTGATTGTTTCTTAGAAGACCATACATTTGAGATGAAGGGCTTCTTCTCTGAGGTCCCTTAAGAACTGAAATGGAATGTATTCTAGGAATACCATAAATTTGGGAGGTTTTCTAAGCCAAATAGTTTGAGAAGGAAAGCCTTGGCAGACTCAAATCTAGTTTTAGTAGTTGATCAAGTGACGATTAATTGTTTCTTGGAAGACAATCAAATGCCGCCTGAGTCAACACAAACGCATGACTTGAAGTGCCTCTCTACCTTTTCTTTATTTTTCTTTCCCACTTTTGTAGAACAATTGATCATCTTTGGTTTCTTGCAAGTAGCAAAAGATCCTTTCAGTATCCAAAAAAGATGCAGCTACATTGGATTCTCCCTAAATCTCTTCTGTTGCAGTTAAGTGCATCAAACTACCCACCATTTGGTTATAAAGTGTGTCGTTCACCTTCTTTCTTCTTTCCTTCACGGTCTTGATTTAGTTCCAAGCCAGACTCAGTTCGAGTATGCATAGGATTGTACTTTGTTTGAAAAGCATTTAAAATTTCTTGCAACAAACCTTTGAAACTGTAAAGATTTTTTTGTACTATTTGGGAGGAAAAGAAAAAGCCTGTGGCTGTTTGTAATATTTCAATACCAAGGAAATAATACATACTTCAAGATCACATCATTTCTAGTATTAGTAAGATCATCTACGTACTAACAAACAATGAGTTCATTGCCTCCATCTCCAGGTTTTGTGGAAAGGTTATGCTCATAATGATATTTCCTGAAAGCTTTCTTTAAGAAATAAATACCAACAATACTACACGAAAGCTCTTGGAGCATGTTTTAGTCTGTTACTTCTTTGAAACTTATACGCATTAGCTCATCTCCAATCTTTTAACGTGGTGATGATTTCAGTTGGTTAATTTCTTTTATCCTTAAATATGCGTTCCACCTAGGGAAGCTCCCGCAAAATTTCCTTAGTGTTGGATAAATTTCCCACACATTTTGACAATGAATGAAAGGTGTCTATGTAACATCTATCATGTGGGATATTGCATATTTGATTTGAAAAATACTTGGCTTGCCTACACACCAGCTGCTGACGGTAGGATACCCTACTGCCTTTCTATTATGGTGTATTTTTAGTGGCTCAAAAAAGTTTAGTGGAGTCTACTTATCTATTAAATGCAATAGAAAGAGACAATAAAAATGATCTATATTAGAAATTAGTTGGATATTTTAATGACTAGTATGTAGGCAGTATTGTTCTTTCAATCCATCTCCCACAAGGAGGAGTAGTTTGAGCTTCTACCATGGGATCCTTATCCTTCATTTCCAACATTAGAATTTATATATATATACAAACAACAATTTCTTCATGTCATTGATAAGACGTAATTACAAAAGAAGGCAAACTATGTGCCGAAGAGGGTATAATTTACACCAAGAGAAAGCAATAGGTATAATTTACACTAGTACAACTTCTTAACCTTGTTTCACCTTTGTTTTCTTCAAGCCTTACAAATGATGAAAATGAGGTTTGAGTCTCTTTTCCCCAAAACTGAATTCTCCAAAAAAAATTAATGAATTTTATGAGTGAATATTACTCATCCTTGATATATTTTTTTAAACAAAGTCTGCTCTCGCTCCAAGACCCAGAATTTGCTCACATAAAGAACTTTCCTTTGTAAGCTCAACTGTAGATTTGAAAACGTGATCTTTGGGTAGGTGTTATACATCCTTGATGTCGACTTATCCCCTGGTCTAGTAATGTCACATTTTTCCAGCTGAAGGAGATTCACGTGTGAGATTTTTGATATGTACCCCTCTATTGGTATTGTGATTTTCCTTCACTTCCTCTAATTGAACCAAATGACCTGGTTTGTAGAACTTGCTGTCTACACTGCTTTCCTTGGGTAACCCTTTATGATATAATGGAAGGGCTCCCAACCTTCCCTCCCCCTCCTACAAGAATCAAGAGTGTATTTTTCCATTGCCATCATTTTTTAAGAATTCATGCTGAAGCATCCTGTCATTCATGCTGAAGCATCCTGTCATTCATGCTGAAGCATCCTGTCAGATATATTCTCCACTCAACAAAATAATCATTACTCGAAATTCTTAGAATAATTTGGAATTAGATTGTACTGAATCTATAGCGGTAGCTTTGGAACAATAACTCAACAACTATATGGATACCTGGTTGATAGAGAGTGACAAGAACCATTGTTTTCTCATTCAAGCCCTGGATAGCTCTCTCCGTACCTACTGGCCACCACGATCTTAAATCAATAAAATGCTTGACTAATCCCACCCTCTCGAAACTCATAACCATAGAGAACCATTGGAAAGGGGGGAAAAGAGAATGAAGAAGATGAAGAAGATGAAGAAGAACAGGGGAATGCATCGTAGGAGTCGCTCTTTTCAAAATTTAACTTTCCACATGTTTAATAAAATCATTAGTTTGTAGGTTTAAATTTAGCTTTGAATCATATGATTATACAAGATTCCTATTGGGAGACAATGACTTTATATTGCACCTAAATACTCGGTACTTCTACTAATGTCTGGGATGAGCTCTATTTAATTAGATTAGACTAAGATGCCACATTTTTTGATACTCTTATTGACTCATATATAATTCAAGATTCAGAAATAGAAACTGTCAAAGCATGACACTACTTAGTAGACTTTTCCTACGTTATTTGTCATGCTAAGACATTTGTGAAACACTTCATCATCCCTATTCTACATCATCCCAAAGCTGGTTTATTGTCTTCACATTAAGTTATGGTTAGGATCCTGCCAAATAGTATCTTTTTTTCAATTTACAAATGATTCACACTTCTGACCAATTACTGAGCATGTTTTATCCTATGCAGTCGTCTTTATTTTGATTTTTAATGCACGGTTAATTTTTCAAGAATGATTCTATAATCTCCTGGAAACTGCAAGGTTTTTGAGTATCTTTATTGGGTAAAAACCTGCAGATAATGTATTGTTATGCAGTGAATGGAAGATGTCCTTCTCCTGGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTGCAAAGGCTTCCAGGATTTGACAAATGGATCATTGAGAGGGAACATAGATCATACATAGATGCTTTGCAGGACCAGAAGGAAAATTTGGTCTATCTCACAGCTGATTCAGAAACTGTGCTTGATGATCTTGATCTAAAGAAAATATATATCATTGGCGGGTTAGTTGATCGGAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCAATAGGAAGTTACTTGAAGATGTCTAGCTCTCAGGTATACTTCGGTCTTCACATGCATATCATTGTTATCCGCATTTCATAG

mRNA sequence

ATGGAGGCCAGAGAAGAGGACCAGAGCGCCAAAATGGATCCTCCTTGTTCCCCTTCCGGCTGCAATCCTCCAGAGCCCCCCCTCTCCAAGAATGCAAGGAAGAAGCTACTGAAACAACAGCGTTACGAAACGAAGAAAGCGGAGAAGAAAGCTCAGGAAAAAGAGCAGAAGAAGAAAGATATTGAGCGCAAACGGAAGGAATGGGAGGAGACACTGGCGAGTGTTACGGAGGAGGAGAGAGCCAAGCTGATCGAGTCTAGGAAAAGCCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGAGAAGGAGAAGAAGATTGAGCGGCTCAACAGGGCTAAAGATTGTGGTCAGAAGATTGTCATCGACCTTGAATTTTCTGACCTCATGACCCCCTCGGAGATTCACAGTCTTGTTCAGCAGATAATGTATTGTTATGCAGTGAATGGAAGATGTCCTTCTCCTGGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTGCAAAGGCTTCCAGGATTTGACAAATGGATCATTGAGAGGGAACATAGATCATACATAGATGCTTTGCAGGACCAGAAGGAAAATTTGGTCTATCTCACAGCTGATTCAGAAACTGTGCTTGATGATCTTGATCTAAAGAAAATATATATCATTGGCGGGTTAGTTGATCGGAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCAATAGGAAGTTACTTGAAGATGTCTAGCTCTCAGGTATACTTCGGTCTTCACATGCATATCATTGTTATCCGCATTTCATAG

Coding sequence (CDS)

ATGGAGGCCAGAGAAGAGGACCAGAGCGCCAAAATGGATCCTCCTTGTTCCCCTTCCGGCTGCAATCCTCCAGAGCCCCCCCTCTCCAAGAATGCAAGGAAGAAGCTACTGAAACAACAGCGTTACGAAACGAAGAAAGCGGAGAAGAAAGCTCAGGAAAAAGAGCAGAAGAAGAAAGATATTGAGCGCAAACGGAAGGAATGGGAGGAGACACTGGCGAGTGTTACGGAGGAGGAGAGAGCCAAGCTGATCGAGTCTAGGAAAAGCCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGAGAAGGAGAAGAAGATTGAGCGGCTCAACAGGGCTAAAGATTGTGGTCAGAAGATTGTCATCGACCTTGAATTTTCTGACCTCATGACCCCCTCGGAGATTCACAGTCTTGTTCAGCAGATAATGTATTGTTATGCAGTGAATGGAAGATGTCCTTCTCCTGGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTGCAAAGGCTTCCAGGATTTGACAAATGGATCATTGAGAGGGAACATAGATCATACATAGATGCTTTGCAGGACCAGAAGGAAAATTTGGTCTATCTCACAGCTGATTCAGAAACTGTGCTTGATGATCTTGATCTAAAGAAAATATATATCATTGGCGGGTTAGTTGATCGGAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCAATAGGAAGTTACTTGAAGATGTCTAGCTCTCAGGTATACTTCGGTCTTCACATGCATATCATTGTTATCCGCATTTCATAG

Protein sequence

MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKDIERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVYFGLHMHIIVIRIS

Homology

[Close]

BLAST of Carg26514 vs. NCBI nr
Match: KAG7019364.1 (trm10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 527.7 bits (1358), Expect = 6.0e-146
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVYFGLHMHIIVIRIS 276
           QGIQTAKLPIGSYLKMSSSQVYFGLHMHIIVIRIS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVYFGLHMHIIVIRIS 275

[Close]

BLAST of Carg26514 vs. NCBI nr
Match: KAG6583717.1 (hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 499.2 bits (1284), Expect = 2.3e-137
Identity = 260/261 (99.62%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYE KKAEKKAQEKEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQV 262
           QGIQTAKLPIGSYLKMSSSQV
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQV 261

[Close]

BLAST of Carg26514 vs. NCBI nr
Match: XP_022927615.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita moschata])

HSP 1 Score: 494.2 bits (1271), Expect = 7.3e-136
Identity = 257/261 (98.47%), Postives = 258/261 (98.85%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYE KKAEKKAQEKEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQD KENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQV 262
           QGIQTAKLPIGSYLKMSSSQV
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQV 261

[Close]

BLAST of Carg26514 vs. NCBI nr
Match: XP_022973151.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima])

HSP 1 Score: 492.3 bits (1266), Expect = 2.8e-135
Identity = 255/261 (97.70%), Postives = 259/261 (99.23%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           MEA EEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYE KKAEKKAQEKEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWE+TLASVTEEERAKLIESR+SLRKERMGKRSEEKEKKIERLNRAKDCGQKI+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQV 262
           QGIQTAKLPIGSYLKMSSSQV
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQV 261

[Close]

BLAST of Carg26514 vs. NCBI nr
Match: XP_023520714.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 492.3 bits (1266), Expect = 2.8e-135
Identity = 257/261 (98.47%), Postives = 258/261 (98.85%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           MEA EEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYE KKAEKKAQEKE KKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKELKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQV 262
           QGIQTAKLPIGSYLKMSSSQV
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQV 261

[Close]

BLAST of Carg26514 vs. ExPASy Swiss-Prot
Match: O14214 (tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=trm10 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.2e-26
Identity = 83/240 (34.58%), Postives = 136/240 (56.67%), Query Frame = 0


Query: 25  EPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKDIERKRKEWEETLASVTEEERAKLI 84
           +P LSK+A K+L +QQ ++  + EK+A+ + +KK+               + +EER + I
Sbjct: 29  QPVLSKSALKRLKRQQEWDAGR-EKRAEMRREKKR---------------LRKEERKRKI 88

Query: 85  ESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYC 144
           E+ + ++           +KK  RL +      +IV+D  F DLM   EI+SL QQ+  C
Sbjct: 89  EAGEVVK----------SQKKRIRLGKVVPSSIRIVLDCAFDDLMNDKEINSLCQQVTRC 148

Query: 145 YAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEREH---RSYIDALQDQKENLVY 204
           ++ N     P  L+ T   G ++T+   +    +   +R +   +SY++  + QKE LVY
Sbjct: 149 HSANRTALHPVELFATNFGGRLKTRQDFVLKGQQNNWKRYNPTTKSYLEEFESQKEKLVY 208

Query: 205 LTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQV 262
           L+ADS+  + +LD  KIYIIG +VD+NR+K +   KA EQGI+TAKLPI  Y+K++  ++
Sbjct: 209 LSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNKASEQGIKTAKLPIDEYIKITDRKI 242

[Close]

BLAST of Carg26514 vs. ExPASy Swiss-Prot
Match: Q8C1Z8 (tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 2.1e-24
Identity = 84/250 (33.60%), Postives = 134/250 (53.60%), Query Frame = 0


Query: 27  PLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKDIERKRKEWEETLASVTEEERAKLIES 86
           P+SK   KKL+KQ+++E ++ ++K + KE++K                            
Sbjct: 39  PISKRQLKKLMKQKQWEEQREQRKEKRKEKRK---------------------------- 98

Query: 87  RKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYCYA 146
           RK L + ++   S+  ++K  R + A+    ++VID  F DLM   +I  L +QI  CYA
Sbjct: 99  RKKLERRQLESNSDGNDRKRVRRDVARS-SLRLVIDCSFDDLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEREHRSYIDALQDQKENLVYL 206
            N R   P   +LT   G+++  + +   G+  W    I+ EH S +     +KE+LVYL
Sbjct: 159 ENRRASHPVQFYLTSHGGQLKKNMDENDQGWVNWKDIHIKSEHYSEL----IKKEDLVYL 218

Query: 207 TADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVY 266
           T+DS  VL DLD  K Y+IGGLVD +  KG+T K+A   GI+ A+LP+  ++KM+S +V 
Sbjct: 219 TSDSPNVLKDLDESKAYVIGGLVDHSHHKGLTFKQATSYGIEHAQLPLADFVKMNSRKVL 255

Query: 267 FGLHMHIIVI 273
              H+  I++
Sbjct: 279 AVNHVFEIIL 255

[Close]

BLAST of Carg26514 vs. ExPASy Swiss-Prot
Match: Q66JJ4 (tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.1e-22
Identity = 100/288 (34.72%), Postives = 152/288 (52.78%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQE---KEQK 60
           +EA  E     +DP       N  +   S++A      Q+     ++E KAQ+   K Q 
Sbjct: 3   LEAVTEPGKCSIDP-------NTKDLLASQHAGNNTPLQENSSAPRSECKAQDAMSKRQM 62

Query: 61  KKDIERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNR------ 120
           KK +  K+K+W        E++R    + RK  R++R  +R  + E  I+  +R      
Sbjct: 63  KKFL--KQKQW--------EDQRELRKQKRKEKRQKRKLERQAQAEHNIDANSRKRFRHE 122

Query: 121 AKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQ 180
            +    +++ID  F DLM   ++  L +QI  CYA N R   P  L+LT   G++++ + 
Sbjct: 123 VQPSALRLIIDCSFDDLMALRDVKKLNKQIRRCYAENRRAVHPVQLYLTSHGGQLKSNMD 182

Query: 181 RLPGFDK-WI------IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGL 240
               +DK WI      I+ EH  Y D +  +KE+LVYLT+DS  VL +LD  K YIIGGL
Sbjct: 183 E---YDKGWINWKDIHIKPEH--YKDLI--KKEDLVYLTSDSPEVLSELDETKAYIIGGL 242

Query: 241 VDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVYFGLHMHIIVI 273
           VD N  KGIT KKA E GI  A+LP+G+++KM++ +V    H+  I++
Sbjct: 243 VDHNHHKGITYKKALELGISHAQLPLGNFVKMNTRKVLAVNHVFEIIL 266

[Close]

BLAST of Carg26514 vs. ExPASy Swiss-Prot
Match: Q3MHI8 (tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.9e-22
Identity = 82/250 (32.80%), Postives = 131/250 (52.40%), Query Frame = 0


Query: 27  PLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKDIERKRKEWEETLASVTEEERAKLIES 86
           P+SK   KKL+KQ+++E ++  +K + KE      +RKRK+ E      +  + +     
Sbjct: 39  PISKRQMKKLMKQKQWEEQRELRKQKRKE------KRKRKQLERQCQPESNSDGS----D 98

Query: 87  RKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYCYA 146
           RK +R++                        +++ID  F  LM   +I  L +QI  CYA
Sbjct: 99  RKRIRRD------------------VVHSPLRLIIDCSFDSLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEREHRSYIDALQDQKENLVYL 206
            N R   P   +LT   G+++  + +   G+  W    I+ EH S       QKE+L+YL
Sbjct: 159 ENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEHYSEF----IQKEDLIYL 218

Query: 207 TADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVY 266
           T+DS  +L +LD  K Y+IGGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +V 
Sbjct: 219 TSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDHGIDHAQLPLGNFVKMNSRKVL 256

Query: 267 FGLHMHIIVI 273
              H+  I++
Sbjct: 279 AVNHVFEIIL 256

[Close]

BLAST of Carg26514 vs. ExPASy Swiss-Prot
Match: Q4KLI2 (tRNA methyltransferase 10 homolog A OS=Rattus norvegicus OX=10116 GN=Trmt10a PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 4.3e-22
Identity = 92/275 (33.45%), Postives = 144/275 (52.36%), Query Frame = 0


Query: 2   EAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKDI 61
           ++ EE Q  ++D             P+SK   KKL+KQ+ +E    E++ Q KE++K+  
Sbjct: 23  DSEEERQKQRVDAEAQ---------PISKRQLKKLMKQRLWE----EQREQRKEKRKE-- 82

Query: 62  ERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVI 121
           +RKRK+ E                     R+ ++   S+  ++K  R + A     +++I
Sbjct: 83  KRKRKKLE---------------------RRCQLESNSDGNDRKRIRRHVAPS-NLRLII 142

Query: 122 DLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW- 181
           D  F DLM   +I  L +QI  CYA N R   P   +LT   G+++  + +   G+  W 
Sbjct: 143 DCSFDDLMVLKDIKKLHKQIQRCYAENRRASHPVQFYLTSHGGQLKKNMDENDQGWVNWK 202

Query: 182 --IIEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKK 241
              I+ EH S +     +KE+LVYLT+DS  VL DLD  K Y+IGGLVD N  KG+T K+
Sbjct: 203 DIHIKSEHYSEL----IKKEDLVYLTSDSPNVLKDLDESKAYVIGGLVDHNHHKGLTFKQ 256

Query: 242 AQEQGIQTAKLPIGSYLKMSSSQVYFGLHMHIIVI 273
           A   GI+ A+LP+  ++KM+S +V    H+  I++
Sbjct: 263 ASSYGIKHAQLPLAEFVKMNSRKVLAVNHVFEIIL 256

[Close]

BLAST of Carg26514 vs. ExPASy TrEMBL
Match: A0A6J1EPG7 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434383 PE=4 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.5e-136
Identity = 257/261 (98.47%), Postives = 258/261 (98.85%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYE KKAEKKAQEKEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQD KENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQV 262
           QGIQTAKLPIGSYLKMSSSQV
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQV 261

[Close]

BLAST of Carg26514 vs. ExPASy TrEMBL
Match: A0A6J1I7W5 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111471663 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.3e-135
Identity = 255/261 (97.70%), Postives = 259/261 (99.23%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           MEA EEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYE KKAEKKAQEKEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWE+TLASVTEEERAKLIESR+SLRKERMGKRSEEKEKKIERLNRAKDCGQKI+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQV 262
           QGIQTAKLPIGSYLKMSSSQV
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQV 261

[Close]

BLAST of Carg26514 vs. ExPASy TrEMBL
Match: A0A0A0LYJ3 (SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G173210 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 6.1e-120
Identity = 232/261 (88.89%), Postives = 243/261 (93.10%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           MEA EE  + KMDPPCS S CNPPEPPLSKNARKKLLKQQRYE KKA+KK Q KEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEE LASVTEEERAKLIESR++LRKERMGKRSE++EKKIERL+ A+D GQ IV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTP EIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+E RSYIDALQ+QKENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQV 262
           Q IQTAKLPIGSYLKMSSSQV
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQV 261

[Close]

BLAST of Carg26514 vs. ExPASy TrEMBL
Match: A0A5D3CW30 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00280 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 5.1e-119
Identity = 229/261 (87.74%), Postives = 241/261 (92.34%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           ME  E   S K+DPPCSPS CNPPEP LSKNARKKLLKQQRYE KKA+KK Q KEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEE LA VTEEERAKLIESR++LRKERMGKRSE++EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+E RSYIDALQ+QKENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQV 262
           Q IQTA+LPIGSYLKMSSSQV
Sbjct: 241 QEIQTARLPIGSYLKMSSSQV 261

[Close]

BLAST of Carg26514 vs. ExPASy TrEMBL
Match: A0A1S3CL37 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502202 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 5.1e-119
Identity = 229/261 (87.74%), Postives = 241/261 (92.34%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKD 60
           ME  E   S K+DPPCSPS CNPPEP LSKNARKKLLKQQRYE KKA+KK Q KEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEE LA VTEEERAKLIESR++LRKERMGKRSE++EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+E RSYIDALQ+QKENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQV 262
           Q IQTA+LPIGSYLKMSSSQV
Sbjct: 241 QEIQTARLPIGSYLKMSSSQV 261

[Close]

BLAST of Carg26514 vs. TAIR 10
Match: AT5G47680.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 348.2 bits (892), Expect = 6.0e-96
Identity = 192/259 (74.13%), Postives = 215/259 (83.01%), Query Frame = 0


Query: 3   AREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYETKKAEKKAQEKEQKKKDIE 62
           A   D +   D P S        PPLSKNA+KK LKQQRYE KKAEKKAQEKE K+K+ E
Sbjct: 4   ATGNDDNGCFDKPESEPAPVNVSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEHKRKEGE 63

Query: 63  RKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVID 122
           RK KEWEETLA+ TEEER KLIESRKSLRKERM KRSEEKEKKIERLN+AK+ GQKIV+D
Sbjct: 64  RKLKEWEETLANATEEERLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEIGQKIVVD 123

Query: 123 LEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIE 182
           ++F+ LM+ SEI SLVQQIMYCYAVNGR  SP HLWLTG  G+M T+L +LPGF+KW IE
Sbjct: 124 VDFAHLMSESEISSLVQQIMYCYAVNGRSTSPCHLWLTGVQGKMSTELDKLPGFEKWFIE 183

Query: 183 REHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQG 242
           +E R YI+A+ DQK+NLVYLTADSETVLDDLD K IYIIGGLVDRNR+KGITM KAQEQG
Sbjct: 184 KESRCYIEAMADQKDNLVYLTADSETVLDDLDPKHIYIIGGLVDRNRFKGITMTKAQEQG 243

Query: 243 IQTAKLPIGSYLKMSSSQV 262
           I+TAKLPIG Y+KMSSSQV
Sbjct: 244 IKTAKLPIGEYMKMSSSQV 262

The following BLAST results are available for this feature:

BLAST of Carg26514 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019364.1	6.0e-146	100.00	trm10, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6583717.1	2.3e-137	99.62	hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022927615.1	7.3e-136	98.47	tRNA (guanine(9)-N1)-methyltransferase [Cucurbita moschata]	[more]
XP_022973151.1	2.8e-135	97.70	tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima]	[more]
XP_023520714.1	2.8e-135	98.47	tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg26514 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O14214	2.2e-26	34.58	tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 ...	[more]
Q8C1Z8	2.1e-24	33.60	tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=...	[more]
Q66JJ4	1.1e-22	34.72	tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=...	[more]
Q3MHI8	1.9e-22	32.80	tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1	[more]
Q4KLI2	4.3e-22	33.45	tRNA methyltransferase 10 homolog A OS=Rattus norvegicus OX=10116 GN=Trmt10a PE=...	[more]

BLAST of Carg26514 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EPG7	3.5e-136	98.47	tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1I7W5	1.3e-135	97.70	tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A0A0LYJ3	6.1e-120	88.89	SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3...	[more]
A0A5D3CW30	5.1e-119	87.74	tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX...	[more]
A0A1S3CL37	5.1e-119	87.74	tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC...	[more]

BLAST of Carg26514 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47680.1	6.0e-96	74.13	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 37..112
None	No IPR available	PIRSR	PIRSR016323-1	PIRSR016323-1	coord: 37..262 e-value: 4.0E-64 score: 214.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..70
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..70
None	No IPR available	PANTHER	PTHR13563:SF17	BNAA06G35330D PROTEIN	coord: 8..262
None	No IPR available	CDD	cd18089	SPOUT_Trm10-like	coord: 117..261 e-value: 8.8085E-70 score: 211.24
IPR038459	tRNA methyltransferase TRM10-type domain superfamily	GENE3D	3.40.1280.30		coord: 97..275 e-value: 1.3E-53 score: 183.6
IPR016009	tRNA methyltransferase TRMD/TRM10-type domain	PFAM	PF01746	tRNA_m1G_MT	coord: 127..258 e-value: 4.5E-21 score: 75.5
IPR007356	tRNA (guanine-N1-)-methyltransferase, eukaryotic	PANTHER	PTHR13563	TRNA (GUANINE-9-) METHYLTRANSFERASE	coord: 8..262
IPR028564	tRNA methyltransferase TRM10-type domain	PROSITE	PS51675	SAM_MT_TRM10	coord: 105..275 score: 36.757744

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26514-RA	Carg26514-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002939	tRNA N1-guanine methylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0052905	tRNA (guanine(9)-N(1))-methyltransferase activity