Carg26300 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26300
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein DCL homolog, chloroplastic-like
Location	Carg_Chr09: 1075577 .. 1079652 (+)
RNA-Seq Expression	Carg26300
Synteny	Carg26300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTACATCCCTCAGCTGGTCTACCGTCTCCACATTAACATTTCATCCTCATGGCTGCTTCTTTGCTACTCCGGGGGCATCCTCTCCTGCGGTTTGGGCTCTGCCATCGCGGGCTTTGTTCTGGGGTCGTGCAGGTGACTCGCCGCTCTTGCTTCACTGCGACGGCGGCGTCTACTCCACCGGACGGGAGCTTAACATCTGCTGAAAATACTGCATCAGTGTCGAGTGCCAGTCAACCAACGAAGTTCCAAAGATGGTGTGAGCCTGATTATCGAAAGTGGAAAGACCAGGAAGACGAAATTCTCAGCGATGTCGAGCCTGTCATATCCCTCACTAAAGAGATTATCCACTCCAATAGGTTCATAATTTTACCCCGAAAGCTTATACTGAATTTTATAGGCATTATCTGTTTGTTTTTTTAGTTGCCGAGGAACTGTGCGAGAAAACAATCAAGTAAAAATATGTTTTTCTTTACATGGTCTGCTCGAATAGAAACTTCCCTAGTCTCGAACAATATATTATATTACAGCAAGATTGGAGAAAAGAATCTTGTTAAAATTCACAAACAGACATACAGAACATGTTTAAGGGTGAGAACCTTGTTAGAAATTTCATTTTTTAATGAACCAAAATATTCCTTCCATGAAACATACATAATACCTTTTCACTCGTGATAAACTCAAGATAATGGAAGATTGTACGTTGATTAGCGGGTAGATGAAAATATAACGTGAAAAGTAAATATGAAGCTGACAATTATACCTTCTTAATAACTGATAATCAGTTATCAGGTTTTCCTTACTTCTAATTTGAATCGACATGAATTGGTATATGTTCTGTGTTTTCATGGCAATATGATAACTACTTTCGTGTATCAACTCCTGGCTAAATCGTTTTAATTGGATCAATGCATTTGATATTTACATACGAGACCCAAAATGTTGAATCTTTCCAAGTACACTGACAACGACGGGAAGCTTTCAAAAACATTACAGCAGAAAGAAAAATGATATTCGAATTAGGTTTCAGTTTATTCTATGTGCCCTTTCTTATCTTTCTTTGTGGATTTGGGATTTGACTTTCTACGGCCTCTGCAAATAACTAGGGGAAGACTTGGTTAATTAATGCGCTCATCTTTCAGTTTTATTTATAGGAGGAGTAGTCAGTTGGACGTTAGGCAGTAGGGCCCTTGAGGTAATTCCGTTTTGATTGGTAGTAGGTGATGTGGAGGAAAAAGGTACTGTGTTTAGTAGTTTCATGTTGGGAGAGATTCAAGCCTCTCGCATTGCTATTTGATCCTCATTGGAATTAGCAAACTTGATTTCTGTTGGAAATTTTATCGACATTTGTTCACATAATATTTGTTAAGGTCTTCTTCCATTTGGTTACTTATTGGAGTCATGATTGTAATCTATATTCTTCTCTTTGATCTTTTCCATTTTGACTAGTATTCAATGCATAATATTATGACAATTGATATCAAAAGTAATGTCCAATGAGACCTAGTGGATGATCAGCAGCTTTTAACCGGTAAAAGCAGTGCTTTCCTCAATGTAGGGTTGGTTGCCAGTGTTCATGAGGTGGTCTCATTAGGCACGTTTCAATACAATATATGGCTCATGTTTTTGTTTTACAATCACACTTTGCTCCTATGATACAATATATGACTTCTATTTTTTGTCCCAGGTATGTGGATGGGGAGCGATTGACATCAGAGGACGAGAGAATTGTGGTTGACAGGCTTCTAGCTCATCATCCACATGCTGAAGATAAAATTGGTTGTGGACTTGAATTCATTATGGTATGCTTTGGTCTGGACTAAATAGTTTAGAGTTCTTGCTATGTTTCAAATATGCAGTTTGCATGCTTTATTTTGTTAGATCATGTAGAAATAAAGCAGATAAAGAAACAAATGACATTTTGTTCGTGGATGTTTCCAAAAGCTTAAAATACAAGAAACTTACGAAATGAATTGGACAACTCTAGCCCTAGAAAATAATTGGATAATAGGTACCCGACTAATATTGTGAGATCCCACCTCGGTTGGGGAGGAGAACTAAATATTCTTTATAAGAGTGTAGAAACCTTTTCCTAACAAACGCGTTTTAAAAATCTGGAGAGGAAACCCGGAAGAGAGTTCAAAGAGGACAATATATGTTAACCATGGGTTTGAGTTGTTACATGTATATATAGCCTTCAGATAAAAAGGATTACAAAGATGGGAAAGAAATTTGAGGTATCCCTCCTTTAACTCTCTCAATTCCAGTTCAAGAGAAACCGTGTAAAATCACATAAATAAATGGTAGCAATTGGCAGAAACCTCTGGCTTTGCACCTCAATCTTTGCCAAAATACTTGCCTGCCCAACTCCCTTCATAACGACCGGATCTAATGAACTTCCTAGCCAATGACTACATAATAACATTTGTTGCATGCCTCCTCAAGTTGCTGCCCATGTGATCAAGTAATAGCCTCCATTTAACAACTATCTCTTTCCATATAAGGGACAGACTTGAATTGTGATTGAGGTCATTAAGCAATTTCATATATACATTACTAATACTGAAGCTTCTCTTAATAATACTTTCGCTGTATGTCATGTGGTACTGGCGTGTCCATTGAAGATCTTTGTGGGCTATCATGCTCTTCTGCGACTGTTTGTACAGAAGAAATGACACAGTTGTAAATGGAACTTATCTTCTGGTGTTGCCAAAGTAGCATACTGGACTCTATGGCTGTGTTTTTGTGTTGCTCAACTATAATCTTGAGTAAGACGTCGTAAATGGAACCACATTTGAAATACGTTGCTGCATGATAGCACCTTGAGTAAGATGTGGTAACTCTGTGGCCCATGGCCTTGCTTTGGCAGTCCTCCACTTCGGCCCTTTCAATGGTTGTCTTATATCCTTTTGGATTCAGGATAGTTGTATCTGTTGGCCAGGCTCTTGGGTTCAATATTATATTCCCTTCACAAAAAATAAACAAAAGTTGAAAATTGCATCTGACCTAATTTTCTGTTTTCCATGACACTTTACCTTCATTGCCCAGCCATACAAAATCATCGCATGCTTTTTTCCTTTGCCCACCCACTTCTCATTTTGAGATGTTGTGCAGGCTACTTTGTTAGACCTCCAATCCAGAAGGTTTGTTCAAGGAAGAACAAAACTGGAATATGGTTGTTTCTAACGTTTAGAAGTATCAGCTTCAGTTAGTTAGTCGGTTTATTTTTTCTTTTAAATTTTTATTTGAGTATTTTGAATTAGCGAGTGATAACTTATTGAGTTAATTATATAACTTCTTAGTTTTGTTTGTATGCAGTGTTTTTGTAAATGCCGGGCATTGTTTTTTTTTTTTCCTTATGTTGTGTGGCTTCTTGAAAAGAATGTGGAGTGATTAATGATCCTCTCAAATGGGGACTCCCATCACACTTTTGATGTCAACAAGGCTTACATCTATTGTCTATAGAGATGTGATGCTTGCCCAGTTTAAAATTGTTCTTTATTTTAAACAATTATTTAAGCTATCTTCTGTCATTGAAGGTTGATCGACACCCCCAATTTCGGCACTCAAGATGCCTCTTTGTTGTAAGGACCGATGGTGGATGGATTGACTTCTCGTATCAAAAGTGTCTTCGTGCATATATCCGAAGTAAATATCCATCATATGCAGAGATGTTTATTCGAGAACATTTCAAACGTGGTAGTTGATGAATGTGTGCTATTGTTGATGCTGAGCTGTGGTCTGAATTTGTTTTGATCAAGAAAGATGGATGAATGATTAAATCAATTCTTCATGGTTCGGTGACCTACATGAAGGAAAAATGTACAGATTGTCAATGTAATAGATAGCATGTCCTGGACGCACTGCACAGTTTTTAAGAATGAGAAGAATCGATGATCATTGAAGTGAACCTTCACTTTATTGACTTGCAGCTCTATTATGTTTGTGGTTATGGATAATGGATACTCAAGGCACCATGGAATTTCTTGTATACGTTCGTTCGAGAACGTCGAATGGTCGAATGGGAATATTGATAATGTTTCAAAGTAATATAGGATTGAAAAGTTTAAACTGAT

mRNA sequence

ATTTTACATCCCTCAGCTGGTCTACCGTCTCCACATTAACATTTCATCCTCATGGCTGCTTCTTTGCTACTCCGGGGGCATCCTCTCCTGCGGTTTGGGCTCTGCCATCGCGGGCTTTGTTCTGGGGTCGTGCAGGTGACTCGCCGCTCTTGCTTCACTGCGACGGCGGCGTCTACTCCACCGGACGGGAGCTTAACATCTGCTGAAAATACTGCATCAGTGTCGAGTGCCAGTCAACCAACGAAGTTCCAAAGATGGTGTGAGCCTGATTATCGAAAGTGGAAAGACCAGGAAGACGAAATTCTCAGCGATGTCGAGCCTGTCATATCCCTCACTAAAGAGATTATCCACTCCAATAGGTATGTGGATGGGGAGCGATTGACATCAGAGGACGAGAGAATTGTGGTTGACAGGCTTCTAGCTCATCATCCACATGCTGAAGATAAAATTGGTTGTGGACTTGAATTCATTATGGTTGATCGACACCCCCAATTTCGGCACTCAAGATGCCTCTTTGTTGTAAGGACCGATGGTGGATGGATTGACTTCTCGTATCAAAAGTGTCTTCGTGCATATATCCGAAGTAAATATCCATCATATGCAGAGATGTTTATTCGAGAACATTTCAAACGTGGTAGTTGATGAATGTGTGCTATTGTTGATGCTGAGCTGTGGTCTGAATTTGTTTTGATCAAGAAAGATGGATGAATGATTAAATCAATTCTTCATGGTTCGGTGACCTACATGAAGGAAAAATGTACAGATTGTCAATGTAATAGATAGCATGTCCTGGACGCACTGCACAGTTTTTAAGAATGAGAAGAATCGATGATCATTGAAGTGAACCTTCACTTTATTGACTTGCAGCTCTATTATGTTTGTGGTTATGGATAATGGATACTCAAGGCACCATGGAATTTCTTGTATACGTTCGTTCGAGAACGTCGAATGGTCGAATGGGAATATTGATAATGTTTCAAAGTAATATAGGATTGAAAAGTTTAAACTGAT

Coding sequence (CDS)

ATGGCTGCTTCTTTGCTACTCCGGGGGCATCCTCTCCTGCGGTTTGGGCTCTGCCATCGCGGGCTTTGTTCTGGGGTCGTGCAGGTGACTCGCCGCTCTTGCTTCACTGCGACGGCGGCGTCTACTCCACCGGACGGGAGCTTAACATCTGCTGAAAATACTGCATCAGTGTCGAGTGCCAGTCAACCAACGAAGTTCCAAAGATGGTGTGAGCCTGATTATCGAAAGTGGAAAGACCAGGAAGACGAAATTCTCAGCGATGTCGAGCCTGTCATATCCCTCACTAAAGAGATTATCCACTCCAATAGGTATGTGGATGGGGAGCGATTGACATCAGAGGACGAGAGAATTGTGGTTGACAGGCTTCTAGCTCATCATCCACATGCTGAAGATAAAATTGGTTGTGGACTTGAATTCATTATGGTTGATCGACACCCCCAATTTCGGCACTCAAGATGCCTCTTTGTTGTAAGGACCGATGGTGGATGGATTGACTTCTCGTATCAAAAGTGTCTTCGTGCATATATCCGAAGTAAATATCCATCATATGCAGAGATGTTTATTCGAGAACATTTCAAACGTGGTAGTTGA

Protein sequence

MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSASQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVDRLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKYPSYAEMFIREHFKRGS

Homology

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BLAST of Carg26300 vs. NCBI nr
Match: KAG7024277.1 (Protein DCL-like, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 404.8 bits (1039), Expect = 4.1e-109
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA
Sbjct: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD
Sbjct: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           PSYAEMFIREHFKRGS
Sbjct: 181 PSYAEMFIREHFKRGS 196

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BLAST of Carg26300 vs. NCBI nr
Match: XP_022936820.1 (protein DCL homolog, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 403.3 bits (1035), Expect = 1.2e-108
Identity = 195/196 (99.49%), Postives = 195/196 (99.49%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVS A
Sbjct: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSGA 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD
Sbjct: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           PSYAEMFIREHFKRGS
Sbjct: 181 PSYAEMFIREHFKRGS 196

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BLAST of Carg26300 vs. NCBI nr
Match: XP_023536078.1 (protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 396.4 bits (1017), Expect = 1.5e-106
Identity = 193/196 (98.47%), Postives = 194/196 (98.98%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           MAASLLLRGHPLLRFGL HRGLCSGVVQVTRRSC TATAASTPPDGSLTSAENTASVSSA
Sbjct: 1   MAASLLLRGHPLLRFGLWHRGLCSGVVQVTRRSCCTATAASTPPDGSLTSAENTASVSSA 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           SQPTK+QRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD
Sbjct: 61  SQPTKYQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           PSYAEMFIREHFKRGS
Sbjct: 181 PSYAEMFIREHFKRGS 196

[Close]

BLAST of Carg26300 vs. NCBI nr
Match: XP_022977112.1 (protein DCL homolog, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 389.0 bits (998), Expect = 2.4e-104
Identity = 189/196 (96.43%), Postives = 193/196 (98.47%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           MAASLLLRGHPLLRFGL HRGLCSGVVQVTRRSC TATAASTPPDGSLTSAENTASVSSA
Sbjct: 1   MAASLLLRGHPLLRFGLWHRGLCSGVVQVTRRSCCTATAASTPPDGSLTSAENTASVSSA 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           SQ TK+QRWCEPDYRKWKDQEDEILSDVEPV+SLTKEI+HSNRYVDGERLTSEDERIVVD
Sbjct: 61  SQSTKYQRWCEPDYRKWKDQEDEILSDVEPVMSLTKEILHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLEFIMVDRHPQFR+SRCLFVVRTDGGWIDFSYQKCLRAYIRSKY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRYSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           PSYAEMFIREHFKRGS
Sbjct: 181 PSYAEMFIREHFKRGS 196

[Close]

BLAST of Carg26300 vs. NCBI nr
Match: XP_022135338.1 (protein DCL, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 354.0 bits (907), Expect = 8.4e-94
Identity = 168/196 (85.71%), Postives = 182/196 (92.86%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           MAASLLLRGHPLLR GL HRGLC+G+VQVTRRSC TATAA TPPDG+LTSAEN  SV S+
Sbjct: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           S P K+ RW EPDYRKWKDQE+E+L+D+EP+ISLTKEI+HSNRYVDGERLTS DERIVV+
Sbjct: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLE IMVDRHPQFRHSRCLFV+RTDGGWIDFSYQKCLRAYIR+KY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           PSYAE FIREHFKRGS
Sbjct: 181 PSYAERFIREHFKRGS 196

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BLAST of Carg26300 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 3.1e-30
Identity = 55/116 (47.41%), Postives = 80/116 (68.97%), Query Frame = 0


Query: 79  DQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVDRLLAHHPHAEDKIGCGLE 138
           D ED+IL    P++   + I+HS +Y + +RL+ E ER +++ LL +HP  E KIGCG++
Sbjct: 99  DWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGID 158

Query: 139 FIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKYPSYAEMFIREHFKR 195
           +IMV  HP F  SRC+F+VR DG  +DFSY KC++  I+ KYP YA+ FI  HF++
Sbjct: 159 YIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of Carg26300 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.4e-29
Identity = 64/174 (36.78%), Postives = 96/174 (55.17%), Query Frame = 0


Query: 21  GLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSASQPTKFQRWCEPDYRKWKDQ 80
           G+ S   ++ R+   +    +T     L   E+   V   S           D   W D 
Sbjct: 56  GIRSDNAELLRKPVISTELETTSESEELVKEESDDEVGKKS----------GDGEGWVDW 115

Query: 81  EDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVDRLLAHHPHAEDKIGCGLEFI 140
           ED+IL D  P++   + I+HS +Y  G+RL+ + +R ++ RLL +HP  + KIG G+++I
Sbjct: 116 EDQILEDTVPLVGFVRMILHSGKYAIGDRLSPDHQRTILQRLLPYHPECDKKIGPGVDYI 175

Query: 141 MVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKYPSYAEMFIREHFKR 195
            V  HP F +SRCLF+VR DG  +DFSY KC++  IR  YP YA+ FI  HF++
Sbjct: 176 TVGYHPDFENSRCLFIVRKDGETVDFSYWKCIKGLIRKNYPLYADSFILRHFRK 219

[Close]

BLAST of Carg26300 vs. ExPASy Swiss-Prot
Match: Q5D869 (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.0e-25
Identity = 53/115 (46.09%), Postives = 78/115 (67.83%), Query Frame = 0


Query: 80   QEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVDRLLAHHPHAEDKIGCGLEF 139
            +E E+LSDVEPV+   ++I+H + Y DG+ ++ +D+  V++++L  HP  E K+G G++F
Sbjct: 1735 EEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDF 1794

Query: 140  IMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKYPSYAEMFIREHFKR 195
            I VD+H  F  SRC FVV TDG   DFSY+K L  Y+  KYP  AE FI ++F +
Sbjct: 1795 ITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]

BLAST of Carg26300 vs. ExPASy Swiss-Prot
Match: Q9LQ02 (DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD1 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 3.1e-06
Identity = 36/110 (32.73%), Postives = 56/110 (50.91%), Query Frame = 0


Query: 87   DVEPVISLTKEIIHSNRYVDGERLTSEDERIVVDRLLAHHPHAEDKIGCGLEFIMVDRHP 146
            ++E +    K I+HS  Y   E L   DE + V  +L  HP++ +KIG G++ I V +  
Sbjct: 1338 NIELLSQSLKRILHS--YEINELLNERDEGL-VKMVLQLHPNSVEKIGPGVKGIRVAK-S 1397

Query: 147  QFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKYPSYAEMFIREHFKRGS 197
            +   S C  VVR DG + DFSY KC+    +   P     +  ++ K G+
Sbjct: 1398 KHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1443

[Close]

BLAST of Carg26300 vs. ExPASy TrEMBL
Match: A0A6J1FET0 (protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111443290 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 5.8e-109
Identity = 195/196 (99.49%), Postives = 195/196 (99.49%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVS A
Sbjct: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSGA 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD
Sbjct: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           PSYAEMFIREHFKRGS
Sbjct: 181 PSYAEMFIREHFKRGS 196

[Close]

BLAST of Carg26300 vs. ExPASy TrEMBL
Match: A0A6J1IHJ1 (protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477278 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.1e-104
Identity = 189/196 (96.43%), Postives = 193/196 (98.47%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           MAASLLLRGHPLLRFGL HRGLCSGVVQVTRRSC TATAASTPPDGSLTSAENTASVSSA
Sbjct: 1   MAASLLLRGHPLLRFGLWHRGLCSGVVQVTRRSCCTATAASTPPDGSLTSAENTASVSSA 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           SQ TK+QRWCEPDYRKWKDQEDEILSDVEPV+SLTKEI+HSNRYVDGERLTSEDERIVVD
Sbjct: 61  SQSTKYQRWCEPDYRKWKDQEDEILSDVEPVMSLTKEILHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLEFIMVDRHPQFR+SRCLFVVRTDGGWIDFSYQKCLRAYIRSKY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRYSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           PSYAEMFIREHFKRGS
Sbjct: 181 PSYAEMFIREHFKRGS 196

[Close]

BLAST of Carg26300 vs. ExPASy TrEMBL
Match: A0A6J1C4J4 (protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007322 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.1e-94
Identity = 168/196 (85.71%), Postives = 182/196 (92.86%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           MAASLLLRGHPLLR GL HRGLC+G+VQVTRRSC TATAA TPPDG+LTSAEN  SV S+
Sbjct: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           S P K+ RW EPDYRKWKDQE+E+L+D+EP+ISLTKEI+HSNRYVDGERLTS DERIVV+
Sbjct: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLE IMVDRHPQFRHSRCLFV+RTDGGWIDFSYQKCLRAYIR+KY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           PSYAE FIREHFKRGS
Sbjct: 181 PSYAERFIREHFKRGS 196

[Close]

BLAST of Carg26300 vs. ExPASy TrEMBL
Match: A0A6J1FNQ4 (protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445919 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.6e-90
Identity = 162/196 (82.65%), Postives = 178/196 (90.82%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           M ASLLLRGHPL+R GL +RGLC+G+VQVTRRSC TA  ASTPP G L+SAENT SV SA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCAGIVQVTRRSCCTAVPASTPPGGGLSSAENTTSVLSA 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           + P K+QRW EP YRKWK+QE+EILSD++P+ISLTKEI+HSNRYVDGERLT EDE+IVVD
Sbjct: 61  TDPPKYQRWNEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLE IMVDRHPQFRHSRCLFV+RTDGGWIDFSYQKCLRAYIR+KY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           P YAE FIREHFKRGS
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of Carg26300 vs. ExPASy TrEMBL
Match: A0A6J1J4G4 (protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481165 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.0e-89
Identity = 159/196 (81.12%), Postives = 177/196 (90.31%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFGLCHRGLCSGVVQVTRRSCFTATAASTPPDGSLTSAENTASVSSA 60
           M ASLLLRGHPL+R GL +RGLC+G++QVTRRSC TA  ASTPP G L+SAENT SV S 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60

Query: 61  SQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120
           + P K+QRW EP YRKWK+QE+EILSD++P+ISLTKEI+HSNRYVDGERLT EDE+IVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180
           RLLAHHPHAEDKIGCGLE IMVDRHPQFRHSRCLFV+RTDGGWIDFSYQKCLRAYIR+KY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAEMFIREHFKRGS 197
           P YA+ FIREHFKRGS
Sbjct: 181 PLYADRFIREHFKRGS 196

[Close]

BLAST of Carg26300 vs. TAIR 10
Match: AT3G46630.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 225.3 bits (573), Expect = 4.2e-59
Identity = 121/210 (57.62%), Postives = 150/210 (71.43%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRFG-LCHR---GLCSGVVQVTRRSCFTATA----------ASTPPDG 60
           M + LLLR  PLLR G L HR   G+ +G +   RR    + A          + +P +G
Sbjct: 1   MTSLLLLRTLPLLRNGFLNHRHQVGIVAGGILSHRRRLLCSLADRPQFYENNDSVSPVEG 60

Query: 61  SLTSAENTASVSSASQPTKFQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVD 120
           S  +A N  S    +      R+ +PDYRKWK+ E EIL D+EP+  L KEI+HS+RY+D
Sbjct: 61  SGAAAMNVTSPVVDNS----WRYEDPDYRKWKNLEAEILRDIEPISLLAKEILHSDRYLD 120

Query: 121 GERLTSEDERIVVDRLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDF 180
           GERL  EDE+IV+++LL +HP+++DKIGCGL+FIMVDRHPQFRHSRCLFVVRTDGGWIDF
Sbjct: 121 GERLDFEDEKIVMEKLLPYHPYSKDKIGCGLDFIMVDRHPQFRHSRCLFVVRTDGGWIDF 180

Query: 181 SYQKCLRAYIRSKYPSYAEMFIREHFKRGS 197
           SYQKCLRAY+R KYPS+AE FIREHFKR S
Sbjct: 181 SYQKCLRAYVRDKYPSHAERFIREHFKRAS 206

[Close]

BLAST of Carg26300 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 133.3 bits (334), Expect = 2.2e-31
Identity = 55/116 (47.41%), Postives = 80/116 (68.97%), Query Frame = 0


Query: 79  DQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVDRLLAHHPHAEDKIGCGLE 138
           D ED+IL    P++   + I+HS +Y + +RL+ E ER +++ LL +HP  E KIGCG++
Sbjct: 99  DWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGID 158

Query: 139 FIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKYPSYAEMFIREHFKR 195
           +IMV  HP F  SRC+F+VR DG  +DFSY KC++  I+ KYP YA+ FI  HF++
Sbjct: 159 YIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of Carg26300 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 132.1 bits (331), Expect = 4.8e-31
Identity = 55/116 (47.41%), Postives = 80/116 (68.97%), Query Frame = 0


Query: 79  DQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVDRLLAHHPHAEDKIGCGLE 138
           D ED+IL    P++   + I+HS +Y + +RL+ E ER +++ LL +HP  E KIGCG++
Sbjct: 99  DWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGID 158

Query: 139 FIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKYPSYAEMFIREHFKR 195
           +IMV  HP F  SRC+F+VR DG  +DFSY KC++  I+ KYP YA+ FI  HF++
Sbjct: 159 YIMVWHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of Carg26300 vs. TAIR 10
Match: AT2G40030.1 (nuclear RNA polymerase D1B )

HSP 1 Score: 118.2 bits (295), Expect = 7.2e-27
Identity = 53/115 (46.09%), Postives = 78/115 (67.83%), Query Frame = 0


Query: 80   QEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVDRLLAHHPHAEDKIGCGLEF 139
            +E E+LSDVEPV+   ++I+H + Y DG+ ++ +D+  V++++L  HP  E K+G G++F
Sbjct: 1735 EEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDF 1794

Query: 140  IMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKYPSYAEMFIREHFKR 195
            I VD+H  F  SRC FVV TDG   DFSY+K L  Y+  KYP  AE FI ++F +
Sbjct: 1795 ITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]

BLAST of Carg26300 vs. TAIR 10
Match: AT1G63020.1 (nuclear RNA polymerase D1A )

HSP 1 Score: 53.5 bits (127), Expect = 2.2e-07
Identity = 36/110 (32.73%), Postives = 56/110 (50.91%), Query Frame = 0


Query: 87   DVEPVISLTKEIIHSNRYVDGERLTSEDERIVVDRLLAHHPHAEDKIGCGLEFIMVDRHP 146
            ++E +    K I+HS  Y   E L   DE + V  +L  HP++ +KIG G++ I V +  
Sbjct: 1338 NIELLSQSLKRILHS--YEINELLNERDEGL-VKMVLQLHPNSVEKIGPGVKGIRVAK-S 1397

Query: 147  QFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKYPSYAEMFIREHFKRGS 197
            +   S C  VVR DG + DFSY KC+    +   P     +  ++ K G+
Sbjct: 1398 KHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1443

The following BLAST results are available for this feature:

BLAST of Carg26300 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024277.1	4.1e-109	100.00	Protein DCL-like, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022936820.1	1.2e-108	99.49	protein DCL homolog, chloroplastic-like [Cucurbita moschata]	[more]
XP_023536078.1	1.5e-106	98.47	protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022977112.1	2.4e-104	96.43	protein DCL homolog, chloroplastic-like [Cucurbita maxima]	[more]
XP_022135338.1	8.4e-94	85.71	protein DCL, chloroplastic isoform X1 [Momordica charantia]	[more]

BLAST of Carg26300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C642	3.1e-30	47.41	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q42463	4.4e-29	36.78	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]
Q5D869	1.0e-25	46.09	DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1...	[more]
Q9LQ02	3.1e-06	32.73	DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD...	[more]

BLAST of Carg26300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FET0	5.8e-109	99.49	protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1IHJ1	1.1e-104	96.43	protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477...	[more]
A0A6J1C4J4	4.1e-94	85.71	protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A6J1FNQ4	1.6e-90	82.65	protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1J4G4	3.0e-89	81.12	protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of Carg26300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46630.1	4.2e-59	57.62	Protein of unknown function (DUF3223)	[more]
AT1G45230.1	2.2e-31	47.41	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	4.8e-31	47.41	Protein of unknown function (DUF3223)	[more]
AT2G40030.1	7.2e-27	46.09	nuclear RNA polymerase D1B	[more]
AT1G63020.1	2.2e-07	32.73	nuclear RNA polymerase D1A	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF11523	DUF3223	coord: 96..172 e-value: 5.3E-28 score: 97.5
None	No IPR available	GENE3D	3.10.450.40		coord: 85..177 e-value: 4.5E-24 score: 86.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..59
None	No IPR available	PANTHER	PTHR33415:SF4	DCL PROTEIN (DUF3223)	coord: 1..195
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 1..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26300-RA	Carg26300-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:1901259	chloroplast rRNA processing
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003729	mRNA binding