Carg26226 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26226
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncytochrome P450 89A2-like
LocationCarg_Chr15: 7230551 .. 7232401 (+)
RNA-Seq ExpressionCarg26226
SyntenyCarg26226
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAAATGAATTGGATTGTGTTCATAATTGTATCCATTTTCATTTCCTTTCTTGTAAAGTTGGTTCTTTTCCCTTTGAGGAGTGGTCCTTACAAGCTCCCACCAGGGCCAACCGCCTTCCCAATACTCACAAACTTCATATGGCTACGCCAGTCGCCTCTTGCAATTGAATCTGTGCTGCGCAGTTTCGTGGCCAAATATGGCCCCATCATCACCATCCATGTCGGCCCGTCCCCGGCCATCATCATCGCCGATCGCTCCATTGTCCACAAAGCCCTCGTCCAAAATGGCGCCATCTTCGCCGACCGCCCTCGAGCTCTCTCCATCAATCGGATCACGGCCTCGGGCAACCTCGACAACATCACCTCCGCCCCTTATAGCCCTACTTGGCGTCTCCTCCGCCGTAATCTTGCAGGGGTAATGCTTCACCCTTCGCGGGTCAGGGGTTATTCCCGAGTCCGTAAAAGGGTGTTGGACATCCTCGTTACTCGTCTTGAATCTCGACCGGAATCGGACACCTTAGTTGTTCGACATTTTCAATTTGCTACGTTTTGTTTGTTGGCTTTGATGTGCTTTGGAGATGAGCTGAATGAGACACAAATCCTGGAAATTCAACAAGTTCAACGTGAGATTGTTGTCAATTTGCAACGCTTTCTTATACTCAATTTTGTGGCCTAAATTGATGAAGATTTTGCTTCGTAAGCGTTGGAAGGAATATTTACAACTCAAGAACAAACAACATGAGGTGGTCAACTCTCCTCGACTTGGATTAAAAAATTTTAAAAATGAAACCTTTAATTATTTATCGGTTCTTGTTCTTGTTCTTGTTCTTAAATAGGTTCTCATCCCGGTAATCAAGGCACGAGAGAAGACCAAACATGAAATAACAAAAGAAGGAGAAGAAAAAGAAGAGAATTTGGTATCATATGTTGATACATTGATTGATTTGCAGCTCATCGACCAAAAAAGAAAGCTTAATAATGAAGAAATAGTAAATTTATGCTCGGAGTTTCTAAACGCCGGGACCGACACCACGACGACAACATTGCAATGGATCATGGCGAATTTAGTGAAGAACCCACAAATCCAACAAAAACTTTTTGAAGAGATCGATGGAATTATGAAATCTAGTACGACACGACATGGATTACGAGAGGAAGAGGTAAAGGAAGAAGATTTAGGGAAGCTTCCATATTTGAAAGCTGTGATTCTAGAAGGGTTACGACGACACCCACCGGGGCATTTCATATTACCCCATGCAGTGAAAGAAGACACGACGTTGGAGAATTACTTCATACCGAAAAACGGGACGGTGAATATCATGTCGGGGGAGATGGGATGGGATCCTGAAGTATGGGAGGATCCAATGACGTTTAAGCCAGAGAGGTTCATGAGTGGCGACGGAGAGGTGTCGTCGACGATGTTCGATATAACCGGGAGCAAGGAGATTAAAATGATGCCGTTCGGAGTCGGGAGGAGGATATGTCCGGGGTATGGTTTAGCAATGCTTCATTTGGAATATTTTGTAGCTAATTTGGTATGGAGATTTGAGTGGAAAGCTGTGGAAGGAGATGAAGTTGATTTATCAGAGAAGATGGAATTCACCATTTGGATGAAGAACCCTCTCAAAGCACAATTACGTCCAAGATTTTAGGACAATATTATATAGTTAATTACTATTTTGTTCACTTTGTTACTATGTTGTTCAAGGGTATTATAGTCTTTTCCTACTCGTCGGGAAAAGTAATTTATGTTTGTAAGCGATCAAAATTGCAATGAAATGAAGTCGATTCTATCTTTTTCGGGTTAGTTTGGACGTTTGATCTTACAAGATTGTATAATTAAGTTATT

mRNA sequence

GGAAAAATGAATTGGATTGTGTTCATAATTGTATCCATTTTCATTTCCTTTCTTGTAAAGTTGGTTCTTTTCCCTTTGAGGAGTGGTCCTTACAAGCTCCCACCAGGGCCAACCGCCTTCCCAATACTCACAAACTTCATATGGCTACGCCAGTCGCCTCTTGCAATTGAATCTGTGCTGCGCAGTTTCGTGGCCAAATATGGCCCCATCATCACCATCCATGTCGGCCCGTCCCCGGCCATCATCATCGCCGATCGCTCCATTGTCCACAAAGCCCTCGTCCAAAATGGCGCCATCTTCGCCGACCGCCCTCGAGCTCTCTCCATCAATCGGATCACGGCCTCGGGCAACCTCGACAACATCACCTCCGCCCCTTATAGCCCTACTTGGCGTCTCCTCCGCCGTAATCTTGCAGGGGTAATGCTTCACCCTTCGCGGGTCAGGGGTTATTCCCGAGTCCGTAAAAGGGTGTTGGACATCCTCGTTACTCGTCTTGAATCTCGACCGGAATCGGACACCTTAGTTGTTCGACATTTTCAATTTGCTACGTTTTGTTTGTTGGCTTTGATGTGCTTTGGAGATGAGCTGAATGAGACACAAATCCTGGAAATTCAACAAGTTCAACGTGAGATTGTTGTCAATTTGCAACGCTTTCTTATACTCAATTTTGTGGCCTAAATTGATGAAGATTTTGCTTCGTAAGCGTTGGAAGGAATATTTACAACTCAAGAACAAACAACATGAGGTTCTCATCCCGGTAATCAAGGCACGAGAGAAGACCAAACATGAAATAACAAAAGAAGGAGAAGAAAAAGAAGAGAATTTGGTATCATATGTTGATACATTGATTGATTTGCAGCTCATCGACCAAAAAAGAAAGCTTAATAATGAAGAAATAGTAAATTTATGCTCGGAGTTTCTAAACGCCGGGACCGACACCACGACGACAACATTGCAATGGATCATGGCGAATTTAGTGAAGAACCCACAAATCCAACAAAAACTTTTTGAAGAGATCGATGGAATTATGAAATCTAGTACGACACGACATGGATTACGAGAGGAAGAGGTAAAGGAAGAAGATTTAGGGAAGCTTCCATATTTGAAAGCTGTGATTCTAGAAGGGTTACGACGACACCCACCGGGGCATTTCATATTACCCCATGCAGTGAAAGAAGACACGACGTTGGAGAATTACTTCATACCGAAAAACGGGACGGTGAATATCATGTCGGGGGAGATGGGATGGGATCCTGAAGTATGGGAGGATCCAATGACGTTTAAGCCAGAGAGGTTCATGAGTGGCGACGGAGAGGTGTCGTCGACGATGTTCGATATAACCGGGAGCAAGGAGATTAAAATGATGCCGTTCGGAGTCGGGAGGAGGATATGTCCGGGGTATGGTTTAGCAATGCTTCATTTGGAATATTTTGTAGCTAATTTGGTATGGAGATTTGAGTGGAAAGCTGTGGAAGGAGATGAAGTTGATTTATCAGAGAAGATGGAATTCACCATTTGGATGAAGAACCCTCTCAAAGCACAATTACGTCCAAGATTTTAGGACAATATTATATAGTTAATTACTATTTTGTTCACTTTGTTACTATGTTGTTCAAGGGTATTATAGTCTTTTCCTACTCGTCGGGAAAAGTAATTTATGTTTGTAAGCGATCAAAATTGCAATGAAATGAAGTCGATTCTATCTTTTTCGGGTTAGTTTGGACGTTTGATCTTACAAGATTGTATAATTAAGTTATT

Coding sequence (CDS)

ATGAGACACAAATCCTGGAAATTCAACAAGTTCAACGTGAGATTGTTGTCAATTTGCAACGCTTTCTTATACTCAATTTTGTGGCCTAAATTGATGAAGATTTTGCTTCGTAAGCGTTGGAAGGAATATTTACAACTCAAGAACAAACAACATGAGGTTCTCATCCCGGTAATCAAGGCACGAGAGAAGACCAAACATGAAATAACAAAAGAAGGAGAAGAAAAAGAAGAGAATTTGGTATCATATGTTGATACATTGATTGATTTGCAGCTCATCGACCAAAAAAGAAAGCTTAATAATGAAGAAATAGTAAATTTATGCTCGGAGTTTCTAAACGCCGGGACCGACACCACGACGACAACATTGCAATGGATCATGGCGAATTTAGTGAAGAACCCACAAATCCAACAAAAACTTTTTGAAGAGATCGATGGAATTATGAAATCTAGTACGACACGACATGGATTACGAGAGGAAGAGGTAAAGGAAGAAGATTTAGGGAAGCTTCCATATTTGAAAGCTGTGATTCTAGAAGGGTTACGACGACACCCACCGGGGCATTTCATATTACCCCATGCAGTGAAAGAAGACACGACGTTGGAGAATTACTTCATACCGAAAAACGGGACGGTGAATATCATGTCGGGGGAGATGGGATGGGATCCTGAAGTATGGGAGGATCCAATGACGTTTAAGCCAGAGAGGTTCATGAGTGGCGACGGAGAGGTGTCGTCGACGATGTTCGATATAACCGGGAGCAAGGAGATTAAAATGATGCCGTTCGGAGTCGGGAGGAGGATATGTCCGGGGTATGGTTTAGCAATGCTTCATTTGGAATATTTTGTAGCTAATTTGGTATGGAGATTTGAGTGGAAAGCTGTGGAAGGAGATGAAGTTGATTTATCAGAGAAGATGGAATTCACCATTTGGATGAAGAACCCTCTCAAAGCACAATTACGTCCAAGATTTTAG

Protein sequence

MRHKSWKFNKFNVRLLSICNAFLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFEEIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRPRF
Homology
BLAST of Carg26226 vs. NCBI nr
Match: KAG7016839.1 (Cytochrome P450 89A2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 661.8 bits (1706), Expect = 3.1e-186
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MRHKSWKFNKFNVRLLSICNAFLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKA 60
           MRHKSWKFNKFNVRLLSICNAFLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKA
Sbjct: 1   MRHKSWKFNKFNVRLLSICNAFLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKA 60

Query: 61  REKTKHEITKEGEEKEENLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTT 120
           REKTKHEITKEGEEKEENLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTT
Sbjct: 61  REKTKHEITKEGEEKEENLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTT 120

Query: 121 TLQWIMANLVKNPQIQQKLFEEIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGL 180
           TLQWIMANLVKNPQIQQKLFEEIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGL
Sbjct: 121 TLQWIMANLVKNPQIQQKLFEEIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGL 180

Query: 181 RRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGD 240
           RRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGD
Sbjct: 181 RRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGD 240

Query: 241 GEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVD 300
           GEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVD
Sbjct: 241 GEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVD 300

Query: 301 LSEKMEFTIWMKNPLKAQLRPRF 324
           LSEKMEFTIWMKNPLKAQLRPRF
Sbjct: 301 LSEKMEFTIWMKNPLKAQLRPRF 323

BLAST of Carg26226 vs. NCBI nr
Match: XP_023550707.1 (cytochrome P450 89A2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 606.7 bits (1563), Expect = 1.2e-169
Identity = 297/302 (98.34%), Postives = 298/302 (98.68%), Query Frame = 0

Query: 22  FLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS 81
           FL   LWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS
Sbjct: 216 FLILNLWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS 275

Query: 82  YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 141
           YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE
Sbjct: 276 YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 335

Query: 142 EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 201
           EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE
Sbjct: 336 EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 395

Query: 202 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF 261
           NYFIPKNGTVNIMSG+MGWDPEVWEDPMTFKPERFMSGDGE SSTMFDITGSKEIKMMPF
Sbjct: 396 NYFIPKNGTVNIMSGDMGWDPEVWEDPMTFKPERFMSGDGEASSTMFDITGSKEIKMMPF 455

Query: 262 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 321
           GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP
Sbjct: 456 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 515

Query: 322 RF 324
           RF
Sbjct: 516 RF 517

BLAST of Carg26226 vs. NCBI nr
Match: KAG6579337.1 (Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 606.3 bits (1562), Expect = 1.5e-169
Identity = 297/302 (98.34%), Postives = 297/302 (98.34%), Query Frame = 0

Query: 22  FLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS 81
           FL   LWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS
Sbjct: 216 FLILNLWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS 275

Query: 82  YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 141
           YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE
Sbjct: 276 YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 335

Query: 142 EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 201
           EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE
Sbjct: 336 EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 395

Query: 202 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF 261
           NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGE SSTMFDITGSKEIKMMPF
Sbjct: 396 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEASSTMFDITGSKEIKMMPF 455

Query: 262 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 321
           GVGRRICPGYG AMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP
Sbjct: 456 GVGRRICPGYGFAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 515

Query: 322 RF 324
           RF
Sbjct: 516 RF 517

BLAST of Carg26226 vs. NCBI nr
Match: XP_022973037.1 (cytochrome P450 89A2-like [Cucurbita maxima])

HSP 1 Score: 598.2 bits (1541), Expect = 4.2e-167
Identity = 292/302 (96.69%), Postives = 296/302 (98.01%), Query Frame = 0

Query: 22  FLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS 81
           FL   LWPKLMKILLRKRWKEYLQLKNKQHEVLIP+IKAREK KHEITKEGEEKEENLVS
Sbjct: 216 FLILNLWPKLMKILLRKRWKEYLQLKNKQHEVLIPIIKAREKVKHEITKEGEEKEENLVS 275

Query: 82  YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 141
           YVDTLIDL+LIDQKRKLNNEEIVNLCSEFLNAGTDTT TTLQWIMANLVKNPQIQQKLFE
Sbjct: 276 YVDTLIDLELIDQKRKLNNEEIVNLCSEFLNAGTDTTATTLQWIMANLVKNPQIQQKLFE 335

Query: 142 EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 201
           EIDGIMKS TTR+GLRE+EVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE
Sbjct: 336 EIDGIMKSCTTRNGLREDEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 395

Query: 202 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF 261
           NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF
Sbjct: 396 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF 455

Query: 262 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 321
           GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP
Sbjct: 456 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 515

Query: 322 RF 324
           RF
Sbjct: 516 RF 517

BLAST of Carg26226 vs. NCBI nr
Match: XP_022922304.1 (cytochrome P450 89A2-like [Cucurbita moschata])

HSP 1 Score: 597.8 bits (1540), Expect = 5.5e-167
Identity = 293/302 (97.02%), Postives = 295/302 (97.68%), Query Frame = 0

Query: 22  FLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS 81
           FL   LWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREK KHEITKEGEEKEENLVS
Sbjct: 216 FLILNLWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKVKHEITKEGEEKEENLVS 275

Query: 82  YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 141
           YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE
Sbjct: 276 YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 335

Query: 142 EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 201
           EIDGIMKSSTTRH LREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE
Sbjct: 336 EIDGIMKSSTTRHELREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 395

Query: 202 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF 261
           NYFIPKNGTVNIMSG+MGWDPEVWEDPMTFKPERF+SGDGE  STMFDITGSKEIKMMPF
Sbjct: 396 NYFIPKNGTVNIMSGDMGWDPEVWEDPMTFKPERFVSGDGEALSTMFDITGSKEIKMMPF 455

Query: 262 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 321
           GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP
Sbjct: 456 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 515

Query: 322 RF 324
           RF
Sbjct: 516 RF 517

BLAST of Carg26226 vs. ExPASy Swiss-Prot
Match: Q42602 (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 371.7 bits (953), Expect = 8.4e-102
Identity = 184/311 (59.16%), Postives = 239/311 (76.85%), Query Frame = 0

Query: 13  VRLLSICNAFLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITK-E 72
           ++LLS+    +++I WPK  K++LRKRW+E+LQ++ +Q +VL+P+I+AR K   E  + E
Sbjct: 206 LQLLSLTKFNIFNI-WPKFTKLILRKRWQEFLQIRRQQRDVLLPLIRARRKIVEERKRSE 265

Query: 73  GEEKEENLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVK 132
            E+K++ + SYVDTL+DL+L ++ RKLN E+I+NLCSEFL AGTDTT T LQWIMANLVK
Sbjct: 266 QEDKKDYVQSYVDTLLDLELPEENRKLNEEDIMNLCSEFLTAGTDTTATALQWIMANLVK 325

Query: 133 NPQIQQKLFEEIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILP 192
            P+IQ++L EEI  ++       G   +EV+EED+ K+PYLKAV+LEGLRRHPPGHF+LP
Sbjct: 326 YPEIQERLHEEIKSVV-------GEEAKEVEEEDVEKMPYLKAVVLEGLRRHPPGHFLLP 385

Query: 193 HAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDIT 252
           H+V EDT L  Y +PKNGT+N M  E+G DP  WE+PM FKPERFM  +  V     D+T
Sbjct: 386 HSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFKPERFMGEEEAV-----DLT 445

Query: 253 GSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWM 312
           GS+ IKMMPFG GRRICPG GLAMLHLEY+VAN+V  F+WK V+G EVDL+EK+EFT+ M
Sbjct: 446 GSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVQGHEVDLTEKLEFTVVM 503

Query: 313 KNPLKAQLRPR 323
           K+PLKA   PR
Sbjct: 506 KHPLKALAVPR 503

BLAST of Carg26226 vs. ExPASy Swiss-Prot
Match: Q9SRQ1 (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.1e-89
Identity = 166/303 (54.79%), Postives = 220/303 (72.61%), Query Frame = 0

Query: 27  LWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVSYVDTL 86
           ++P + K LLR++WKE+L+L+  Q  V++  + AR K          E   +++ YVDTL
Sbjct: 224 IFPSVTKFLLRRKWKEFLELRKSQESVILRYVNARSK----------ETTGDVLCYVDTL 283

Query: 87  IDLQLIDQ------KRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLF 146
           ++L++  +      KRKL++ EIV+LCSEFLNA TD T T++QWIMA +VK P+IQ+K++
Sbjct: 284 LNLEIPTEEKEGGKKRKLSDSEIVSLCSEFLNAATDPTATSMQWIMAIMVKYPEIQRKVY 343

Query: 147 EEIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTL 206
           EE    MK+         EE++EEDLGKL YLKAVILE LRRHPPGH++  H V  DT L
Sbjct: 344 EE----MKTVFAGEEEEREEIREEDLGKLSYLKAVILECLRRHPPGHYLSYHKVTHDTVL 403

Query: 207 ENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMP 266
             + IP+ GT+N M GEMG DP++WEDP+TFKPERF+  +GE     FD+TG++EIKMMP
Sbjct: 404 GGFLIPRQGTINFMVGEMGRDPKIWEDPLTFKPERFLE-NGEACD--FDMTGTREIKMMP 463

Query: 267 FGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEF-TIWMKNPLKAQL 323
           FG GRR+CPGY L++LHLEY+VANLVW+FEWK VEG+EVDLSEK +F T+ MKNP KA +
Sbjct: 464 FGAGRRMCPGYALSLLHLEYYVANLVWKFEWKCVEGEEVDLSEKQQFITMVMKNPFKANI 509

BLAST of Carg26226 vs. ExPASy Swiss-Prot
Match: P37123 (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.8e-62
Identity = 127/286 (44.41%), Postives = 179/286 (62.59%), Query Frame = 0

Query: 38  KRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVSYVDTLIDLQLIDQKRK 97
           K+ K   +++ +Q E L+P+I   EK +  +   G +K     SY+DTL D+++  +K  
Sbjct: 230 KQRKRVNEVRKRQIETLVPLI---EKRRSVVQNPGSDKTAASFSYLDTLFDVKVEGRKSG 289

Query: 98  LNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFEEIDGIMKSSTTRHGLR 157
             N E+V LCSEFLN GTDTT T L+W +  L++NP IQ +L++EI  I         + 
Sbjct: 290 PTNAELVTLCSEFLNGGTDTTATALEWGIGRLMENPTIQNQLYQEIKTI---------VG 349

Query: 158 EEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGE 217
           +++V E D+ K+PYL AV+ E LR+HPP +F L H+V E   L  Y IP +  V      
Sbjct: 350 DKKVDENDIEKMPYLNAVVKELLRKHPPTYFTLTHSVTEPVKLAGYDIPMDTNVEFFVHG 409

Query: 218 MGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLH 277
           +  DP VW DP  F P+RF+SG  +      DITG KE+KMMPFGVGRRICPG G+A +H
Sbjct: 410 ISHDPNVWSDPEKFDPDRFLSGREDA-----DITGVKEVKMMPFGVGRRICPGLGMATVH 469

Query: 278 LEYFVANLVWRFEWKAVEG-DEVDLSEKMEFTIWMKNPLKAQLRPR 323
           +   +A +V  FEW A  G ++VD SEK+EFT+ MKNPL+A+++ R
Sbjct: 470 VNLMLARMVQEFEWFAYPGNNKVDFSEKLEFTVVMKNPLRAKVKLR 498

BLAST of Carg26226 vs. ExPASy Swiss-Prot
Match: O48928 (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.2e-61
Identity = 124/286 (43.36%), Postives = 180/286 (62.94%), Query Frame = 0

Query: 38  KRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVSYVDTLIDLQLIDQKRK 97
           K+ K+ L+++ +Q E L+P+I+ R +    I   G +      SY+DTL DL++  +K  
Sbjct: 238 KQRKKALEVRREQVEFLVPIIEQRRRA---IQNPGSDHTATTFSYLDTLFDLKVEGKKSA 297

Query: 98  LNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFEEIDGIMKSSTTRHGLR 157
            ++ E+V+LCSEFLN GTDTT T ++W +A L+ NP +Q KL+EEI         +  + 
Sbjct: 298 PSDAELVSLCSEFLNGGTDTTATAVEWGIAQLIANPNVQTKLYEEI---------KRTVG 357

Query: 158 EEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGE 217
           E++V E+D+ K+PYL AV+ E LR+HPP HF+L HAV E TTL  Y IP +  V + +  
Sbjct: 358 EKKVDEKDVEKMPYLHAVVKELLRKHPPTHFVLTHAVTEPTTLGGYDIPIDANVEVYTPA 417

Query: 218 MGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLH 277
           +  DP+ W +P  F PERF+SG  E      DITG   +KMMPFGVGRRICPG  +A +H
Sbjct: 418 IAEDPKNWLNPEKFDPERFISGGEEA-----DITGVTGVKMMPFGVGRRICPGLAMATVH 477

Query: 278 LEYFVANLVWRFEWKAVEGD-EVDLSEKMEFTIWMKNPLKAQLRPR 323
           +   +A +V  FEW A   + ++D + K EFT+ MK  L+A ++PR
Sbjct: 478 IHLMMARMVQEFEWGAYPPEKKMDFTGKWEFTVVMKESLRATIKPR 506

BLAST of Carg26226 vs. ExPASy Swiss-Prot
Match: Q9LZ31 (Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 4.0e-59
Identity = 120/286 (41.96%), Postives = 175/286 (61.19%), Query Frame = 0

Query: 38  KRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVSYVDTLIDLQLIDQKRK 97
           K  K  L+++ +Q + ++ VI   E+ +  I   G +K  +  SY+DTL DL++  +K  
Sbjct: 242 KERKRALEVRREQVDYVVGVI---ERRRRAIQNPGSDKTASSFSYLDTLFDLKIEGRKTT 301

Query: 98  LNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFEEIDGIMKSSTTRHGLR 157
            +NEE+V LCSEFLN GTDTT T ++W +A L+ NP+IQ +L++EI   +          
Sbjct: 302 PSNEELVTLCSEFLNGGTDTTGTAIEWGIAQLIANPEIQSRLYDEIKSTVGD-------- 361

Query: 158 EEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLENYFIPKNGTVNIMSGE 217
           +  V E+D+ K+ +L+A + E LR+HPP +F L HAV E TTL  Y IP    V +    
Sbjct: 362 DRRVDEKDVDKMVFLQAFVKELLRKHPPTYFSLTHAVMETTTLAGYDIPAGVNVEVYLPG 421

Query: 218 MGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPFGVGRRICPGYGLAMLH 277
           +  DP +W +P  F P+RFM G  +      DITG   +KM+PFGVGRRICPG  +A +H
Sbjct: 422 ISEDPRIWNNPKKFDPDRFMLGKEDA-----DITGISGVKMIPFGVGRRICPGLAMATIH 481

Query: 278 LEYFVANLVWRFEWKA-VEGDEVDLSEKMEFTIWMKNPLKAQLRPR 323
           +   +A +V  FEW A   G E+D + K+EFT+ MKNPL+A ++PR
Sbjct: 482 VHLMLARMVQEFEWCAHPPGSEIDFAGKLEFTVVMKNPLRAMVKPR 511

BLAST of Carg26226 vs. ExPASy TrEMBL
Match: A0A6J1I6F9 (cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111471545 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.0e-167
Identity = 292/302 (96.69%), Postives = 296/302 (98.01%), Query Frame = 0

Query: 22  FLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS 81
           FL   LWPKLMKILLRKRWKEYLQLKNKQHEVLIP+IKAREK KHEITKEGEEKEENLVS
Sbjct: 216 FLILNLWPKLMKILLRKRWKEYLQLKNKQHEVLIPIIKAREKVKHEITKEGEEKEENLVS 275

Query: 82  YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 141
           YVDTLIDL+LIDQKRKLNNEEIVNLCSEFLNAGTDTT TTLQWIMANLVKNPQIQQKLFE
Sbjct: 276 YVDTLIDLELIDQKRKLNNEEIVNLCSEFLNAGTDTTATTLQWIMANLVKNPQIQQKLFE 335

Query: 142 EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 201
           EIDGIMKS TTR+GLRE+EVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE
Sbjct: 336 EIDGIMKSCTTRNGLREDEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 395

Query: 202 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF 261
           NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF
Sbjct: 396 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF 455

Query: 262 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 321
           GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP
Sbjct: 456 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 515

Query: 322 RF 324
           RF
Sbjct: 516 RF 517

BLAST of Carg26226 vs. ExPASy TrEMBL
Match: A0A6J1E305 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430325 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.7e-167
Identity = 293/302 (97.02%), Postives = 295/302 (97.68%), Query Frame = 0

Query: 22  FLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS 81
           FL   LWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREK KHEITKEGEEKEENLVS
Sbjct: 216 FLILNLWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKVKHEITKEGEEKEENLVS 275

Query: 82  YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 141
           YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE
Sbjct: 276 YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 335

Query: 142 EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 201
           EIDGIMKSSTTRH LREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE
Sbjct: 336 EIDGIMKSSTTRHELREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 395

Query: 202 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF 261
           NYFIPKNGTVNIMSG+MGWDPEVWEDPMTFKPERF+SGDGE  STMFDITGSKEIKMMPF
Sbjct: 396 NYFIPKNGTVNIMSGDMGWDPEVWEDPMTFKPERFVSGDGEALSTMFDITGSKEIKMMPF 455

Query: 262 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 321
           GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP
Sbjct: 456 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 515

Query: 322 RF 324
           RF
Sbjct: 516 RF 517

BLAST of Carg26226 vs. ExPASy TrEMBL
Match: A0A6J1E669 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430324 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.9e-166
Identity = 291/301 (96.68%), Postives = 294/301 (97.67%), Query Frame = 0

Query: 22  FLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKEENLVS 81
           FL    WPKLMKILLRKRWKEYLQLKNKQHEVLIP+IKAREK KHEITKEGEEKEENLVS
Sbjct: 216 FLILNFWPKLMKILLRKRWKEYLQLKNKQHEVLIPIIKAREKVKHEITKEGEEKEENLVS 275

Query: 82  YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 141
           YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE
Sbjct: 276 YVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFE 335

Query: 142 EIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 201
           EIDGIMKSSTT+H L EEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE
Sbjct: 336 EIDGIMKSSTTQHELLEEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLE 395

Query: 202 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPF 261
           NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSG+GEVSSTMFDITGSKEIKMMPF
Sbjct: 396 NYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGEGEVSSTMFDITGSKEIKMMPF 455

Query: 262 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 321
           GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP
Sbjct: 456 GVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRP 515

Query: 322 R 323
           R
Sbjct: 516 R 516

BLAST of Carg26226 vs. ExPASy TrEMBL
Match: A0A1S3ATZ4 (cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103482935 PE=3 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.8e-131
Identity = 238/307 (77.52%), Postives = 265/307 (86.32%), Query Frame = 0

Query: 22  FLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITK-EGEEKEENLV 81
           F+   LWPKLMKILLR RWKEYLQ+KN+Q +VL+P IKAREK K E  K EGEEKEE +V
Sbjct: 220 FVLLNLWPKLMKILLRNRWKEYLQIKNRQKKVLVPFIKAREKVKEERAKREGEEKEEFVV 279

Query: 82  SYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLF 141
           SYVDTL+DLQLID+KRKL NEEIV+LCSEFLNAGTDTT T++QWIMANLVK P+IQ+KLF
Sbjct: 280 SYVDTLLDLQLIDEKRKLKNEEIVSLCSEFLNAGTDTTATSMQWIMANLVKYPKIQEKLF 339

Query: 142 EEIDGIMKSSTTRH-GLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTT 201
           +EI G+ +SST ++ GLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDT 
Sbjct: 340 QEIKGVKESSTKKNGGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTI 399

Query: 202 LENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVS---STMFDITGSKEI 261
            ENYFIPKNG V+I +  MGWDP+VWEDPM FKPERFMS DG      ST FDITG+KEI
Sbjct: 400 FENYFIPKNGIVSIFAVGMGWDPQVWEDPMAFKPERFMSDDGGREAGYSTTFDITGNKEI 459

Query: 262 KMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLK 321
           KMMPFG GRR+CP   LAMLHLEYFVANL+WRFEWKAVEG+EVDLSEKMEFT+ MK PLK
Sbjct: 460 KMMPFGAGRRMCPAASLAMLHLEYFVANLIWRFEWKAVEGEEVDLSEKMEFTVVMKKPLK 519

Query: 322 AQLRPRF 324
           A + PRF
Sbjct: 520 AHINPRF 526

BLAST of Carg26226 vs. ExPASy TrEMBL
Match: A0A0A0KJJ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139780 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.2e-129
Identity = 237/307 (77.20%), Postives = 266/307 (86.64%), Query Frame = 0

Query: 22  FLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHE-ITKEGEEKEENLV 81
           F++  LWPKLMK+LLR RWKEYLQ+KN+Q +VL+P IKAREK K E   KEGEE EE +V
Sbjct: 219 FIFLNLWPKLMKVLLRNRWKEYLQIKNRQRKVLVPFIKAREKIKEERAKKEGEENEEFVV 278

Query: 82  SYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLF 141
           SYVDTL+DLQLID+KRKLNNEEIVNLCSEFLNAGTDTT T+LQWIMANLVK P+IQ+KLF
Sbjct: 279 SYVDTLLDLQLIDEKRKLNNEEIVNLCSEFLNAGTDTTATSLQWIMANLVKYPKIQEKLF 338

Query: 142 EEIDGIMKS-STTRHGLR-EEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDT 201
           +EI G+ +S ST + GLR EEEVKEEDLGKLPYLKAVILEGLRRHPPGHF++PHAVKEDT
Sbjct: 339 QEIKGVKESASTKKDGLREEEEVKEEDLGKLPYLKAVILEGLRRHPPGHFMVPHAVKEDT 398

Query: 202 TLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVS--STMFDITGSKEI 261
           TLENYFIPKNG V+  +  MG DP+VWEDPM FKPERFMS DG  +  ST FDITG+KEI
Sbjct: 399 TLENYFIPKNGIVSFFAVGMGLDPQVWEDPMAFKPERFMSDDGGKAEYSTTFDITGNKEI 458

Query: 262 KMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLK 321
           KMMPFG GRR+CPG  LA+LHLEYFVANLVWRFEWKAVEG+EVDLSEKMEFTI MK PL+
Sbjct: 459 KMMPFGAGRRMCPGTSLAILHLEYFVANLVWRFEWKAVEGEEVDLSEKMEFTIVMKKPLR 518

Query: 322 AQLRPRF 324
           A +  RF
Sbjct: 519 AHINERF 525

BLAST of Carg26226 vs. TAIR 10
Match: AT1G64950.1 (cytochrome P450, family 89, subfamily A, polypeptide 5 )

HSP 1 Score: 376.3 bits (965), Expect = 2.4e-104
Identity = 182/300 (60.67%), Postives = 232/300 (77.33%), Query Frame = 0

Query: 27  LWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAR----EKTKHEITKEGEEKEENLVSY 86
           LWPK  K++LRKRW+E+ Q++ +QH+VL+P+I+AR    E+ K+  ++E E+ +E + SY
Sbjct: 222 LWPKFTKLILRKRWEEFFQMRREQHDVLLPLIRARRKIVEERKNRSSEEEEDNKEYVQSY 281

Query: 87  VDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFEE 146
           VDTL++L+L D+KRKLN +EIV+LCSEFLN GTDTT T LQWIMANLVKNP IQ++L+EE
Sbjct: 282 VDTLLELELPDEKRKLNEDEIVSLCSEFLNGGTDTTATALQWIMANLVKNPDIQKRLYEE 341

Query: 147 IDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLEN 206
           I  ++       G    EV+EED  K+PYL+AV++EGLRRHPPGHF+LPH+V EDT L  
Sbjct: 342 IKSVV-------GEEANEVEEEDAQKMPYLEAVVMEGLRRHPPGHFVLPHSVTEDTVLGG 401

Query: 207 YFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPFG 266
           Y +PKNGT+N M  E+G DP+VWE+PM FKPERFM           DITGS+ IKMMPFG
Sbjct: 402 YKVPKNGTINFMVAEIGRDPKVWEEPMAFKPERFM-------EEAVDITGSRGIKMMPFG 461

Query: 267 VGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRPR 323
            GRRICPG GLAMLHLEY+VAN+V  F+WK V+G EVDL+EK+EFT+ MK+PLKA   PR
Sbjct: 462 AGRRICPGIGLAMLHLEYYVANMVREFDWKEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 507

BLAST of Carg26226 vs. TAIR 10
Match: AT2G12190.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 4.6e-103
Identity = 183/295 (62.03%), Postives = 228/295 (77.29%), Query Frame = 0

Query: 27  LWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHE-ITKEGEEKEENLV---SY 86
           LWPK  K++LRKRW+E+ Q++ +QH+VL+P+I+AR K   E   +  EE+E+N V   SY
Sbjct: 222 LWPKFTKLILRKRWEEFFQMRREQHDVLLPLIRARRKIVEERKNRSSEEEEDNKVYVQSY 281

Query: 87  VDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFEE 146
           VDTL++L+L D+KRKLN +EIV+LCSEFLN GTDTT T LQWIMANLVKNP+IQ++L+EE
Sbjct: 282 VDTLLELELPDEKRKLNEDEIVSLCSEFLNGGTDTTATALQWIMANLVKNPEIQKRLYEE 341

Query: 147 IDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLEN 206
           I  ++       G   +EV+EED  K+PYLKAV++EGLRRHPPGHF+LPH+V EDT L  
Sbjct: 342 IKSVV-------GEEAKEVEEEDAQKMPYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGG 401

Query: 207 YFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPFG 266
           Y +PK GT+N M  E+G DP VWE+PM FKPERFM  +  V     DITGS+ IKMMPFG
Sbjct: 402 YKVPKKGTINFMVAEIGRDPMVWEEPMAFKPERFMGEEEAV-----DITGSRGIKMMPFG 461

Query: 267 VGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKA 318
            GRRICPG GLAMLHLEY+VAN+V  FEWK V+G EVDL+EK EFT+ MK+ LKA
Sbjct: 462 AGRRICPGIGLAMLHLEYYVANMVREFEWKEVQGHEVDLTEKFEFTVVMKHSLKA 504

BLAST of Carg26226 vs. TAIR 10
Match: AT1G64900.1 (cytochrome P450, family 89, subfamily A, polypeptide 2 )

HSP 1 Score: 371.7 bits (953), Expect = 6.0e-103
Identity = 184/311 (59.16%), Postives = 239/311 (76.85%), Query Frame = 0

Query: 13  VRLLSICNAFLYSILWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITK-E 72
           ++LLS+    +++I WPK  K++LRKRW+E+LQ++ +Q +VL+P+I+AR K   E  + E
Sbjct: 206 LQLLSLTKFNIFNI-WPKFTKLILRKRWQEFLQIRRQQRDVLLPLIRARRKIVEERKRSE 265

Query: 73  GEEKEENLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVK 132
            E+K++ + SYVDTL+DL+L ++ RKLN E+I+NLCSEFL AGTDTT T LQWIMANLVK
Sbjct: 266 QEDKKDYVQSYVDTLLDLELPEENRKLNEEDIMNLCSEFLTAGTDTTATALQWIMANLVK 325

Query: 133 NPQIQQKLFEEIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILP 192
            P+IQ++L EEI  ++       G   +EV+EED+ K+PYLKAV+LEGLRRHPPGHF+LP
Sbjct: 326 YPEIQERLHEEIKSVV-------GEEAKEVEEEDVEKMPYLKAVVLEGLRRHPPGHFLLP 385

Query: 193 HAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDIT 252
           H+V EDT L  Y +PKNGT+N M  E+G DP  WE+PM FKPERFM  +  V     D+T
Sbjct: 386 HSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFKPERFMGEEEAV-----DLT 445

Query: 253 GSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWM 312
           GS+ IKMMPFG GRRICPG GLAMLHLEY+VAN+V  F+WK V+G EVDL+EK+EFT+ M
Sbjct: 446 GSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVQGHEVDLTEKLEFTVVM 503

Query: 313 KNPLKAQLRPR 323
           K+PLKA   PR
Sbjct: 506 KHPLKALAVPR 503

BLAST of Carg26226 vs. TAIR 10
Match: AT1G64940.1 (cytochrome P450, family 87, subfamily A, polypeptide 6 )

HSP 1 Score: 370.9 bits (951), Expect = 1.0e-102
Identity = 182/300 (60.67%), Postives = 229/300 (76.33%), Query Frame = 0

Query: 27  LWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAREKTKHEITKEGEEKE----ENLVSY 86
           LWPK  K++ RKRW+E+ Q++++Q +VL+P+I+AR K   E  K   E+E    E + SY
Sbjct: 223 LWPKFTKLIYRKRWEEFFQMQSEQQDVLLPLIRARRKIVDERKKRSSEEEKDNKEYVQSY 282

Query: 87  VDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQKLFEE 146
           VDTL+D++L D+KRKLN +EIV+LCSEFLNAGTDTT T LQWIMANLVKNP+IQ++L+EE
Sbjct: 283 VDTLLDVELPDEKRKLNEDEIVSLCSEFLNAGTDTTATALQWIMANLVKNPEIQRRLYEE 342

Query: 147 IDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKEDTTLEN 206
           I  I+       G   +EV+E+D  K+PYLKAV++EGLRRHPPGHF+LPH+V EDT L  
Sbjct: 343 IKSIV-------GEEAKEVEEQDAQKMPYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGG 402

Query: 207 YFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIKMMPFG 266
           Y +PK GT+N M  E+G DP+VWE+PM FKPERFM           DITGS+ IKMMPFG
Sbjct: 403 YKVPKKGTINFMVAEIGRDPKVWEEPMAFKPERFM-------EEAVDITGSRGIKMMPFG 462

Query: 267 VGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKAQLRPR 323
            GRRICPG GLAMLHLEY+VAN+V  FEW+ V+G EVDL+EK+EFT+ MK+PLKA   PR
Sbjct: 463 AGRRICPGIGLAMLHLEYYVANMVREFEWQEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 508

BLAST of Carg26226 vs. TAIR 10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7 )

HSP 1 Score: 348.2 bits (892), Expect = 7.1e-96
Identity = 177/305 (58.03%), Postives = 229/305 (75.08%), Query Frame = 0

Query: 24  YSI--LWPKLMKILLRKRWKEYLQLKNKQHEVLIPVIKAR----EKTKHEITKEGEEKEE 83
           YSI  L PK  K++LRKRW+E+ Q++ +Q +VL+ +I AR    E+ K   ++E EE +E
Sbjct: 216 YSILNLCPKFTKLILRKRWEEFFQMRREQQDVLLRLIYARRKIVEERKKRSSEEEEENKE 275

Query: 84  NLVSYVDTLIDLQLIDQKRKLNNEEIVNLCSEFLNAGTDTTTTTLQWIMANLVKNPQIQQ 143
            + SYVDTL+D++L D+KRKLN +EIV+LCSEFL AG+DTT T LQWIMANLVKN +IQ+
Sbjct: 276 YVQSYVDTLLDVELPDEKRKLNEDEIVSLCSEFLIAGSDTTATVLQWIMANLVKNQEIQE 335

Query: 144 KLFEEIDGIMKSSTTRHGLREEEVKEEDLGKLPYLKAVILEGLRRHPPGHFILPHAVKED 203
           +L+EEI  ++       G   + V+E+D  K+PYLKAV++E LRRHPPG+ +LPH+V ED
Sbjct: 336 RLYEEITNVV-------GEEAKVVEEKDTQKMPYLKAVVMEALRRHPPGNTVLPHSVTED 395

Query: 204 TTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGEVSSTMFDITGSKEIK 263
           T L  Y +PK GT+N +  E+G DP+VWE+PM FKPERFM  +  V     DITGS+ IK
Sbjct: 396 TVLGGYKVPKKGTINFLVAEIGRDPKVWEEPMAFKPERFMGEEEAV-----DITGSRGIK 455

Query: 264 MMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKMEFTIWMKNPLKA 323
           MMPFG GRRICPG GLAMLHLEY+VAN+V  F+WK VEG EVDL+EK+EFT+ MK+PLKA
Sbjct: 456 MMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVEGHEVDLTEKVEFTVIMKHPLKA 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7016839.13.1e-186100.00Cytochrome P450 89A2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023550707.11.2e-16998.34cytochrome P450 89A2-like [Cucurbita pepo subsp. pepo][more]
KAG6579337.11.5e-16998.34Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022973037.14.2e-16796.69cytochrome P450 89A2-like [Cucurbita maxima][more]
XP_022922304.15.5e-16797.02cytochrome P450 89A2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q426028.4e-10259.16Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2[more]
Q9SRQ12.1e-8954.79Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1[more]
P371233.8e-6244.41Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=... [more]
O489287.2e-6143.36Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1[more]
Q9LZ314.0e-5941.96Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I6F92.0e-16796.69cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111471545 PE=3 SV=1[more]
A0A6J1E3052.7e-16797.02cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430325 PE=3 SV=... [more]
A0A6J1E6692.9e-16696.68cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430324 PE=3 SV=... [more]
A0A1S3ATZ41.8e-13177.52cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103482935 PE=3 SV=1[more]
A0A0A0KJJ92.2e-12977.20Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139780 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G64950.12.4e-10460.67cytochrome P450, family 89, subfamily A, polypeptide 5 [more]
AT2G12190.14.6e-10362.03Cytochrome P450 superfamily protein [more]
AT1G64900.16.0e-10359.16cytochrome P450, family 89, subfamily A, polypeptide 2 [more]
AT1G64940.11.0e-10260.67cytochrome P450, family 87, subfamily A, polypeptide 6 [more]
AT1G64930.17.1e-9658.03cytochrome P450, family 87, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 258..268
score: 56.12
coord: 102..119
score: 28.01
coord: 122..148
score: 32.95
coord: 173..191
score: 33.95
coord: 268..291
score: 33.01
coord: 214..238
score: 32.91
IPR001128Cytochrome P450PRINTSPR00385P450coord: 268..279
score: 38.04
coord: 113..130
score: 41.92
coord: 259..268
score: 59.05
coord: 174..185
score: 43.75
IPR001128Cytochrome P450PFAMPF00067p450coord: 7..314
e-value: 1.8E-69
score: 234.7
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 3..323
e-value: 2.2E-79
score: 269.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 14..322
NoneNo IPR availablePANTHERPTHR24298:SF800CYTOCHROME P450 89A2-RELATEDcoord: 23..319
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 23..319
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 261..270

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26226-RACarg26226-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen