Carg26080 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26080
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	heat stress transcription factor B-3-like
Location	Carg_Chr06: 5145244 .. 5146052 (+)
RNA-Seq Expression	Carg26080
Synteny	Carg26080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TATATCTTCTTCTTCCTTTCTGCTTCCACTTCATTTCTCTCATGGGGGAGCAGCCAGCTATAGGCCTAGGTGATGTGTGTCAAAAGCCAAAGCCCTCTCCGCCGCCCTTCTTGGCCAAAACCTACCTGTTGGTCGAGGATCCGACCACCGATGATGTCATCTCCTGGAATGACGATGGGACGGCGCTCGTCGTGTGGCATCCCCCAGAGTTCGCTAGAGATCTCCTCCCTACCCTCTTCAAACATAGCAACTTCTCTAGCTTTGTTCGCCAACTAAACACATATGTAAGTCGATATAACATGAAATTTATCGTAACCCATTAGCTTAAGTTTGACGGAGAATCGAACCTTACAACAAAACTTTGATAGGGATTTCGCAAGATTGCGACAAGTCGATGGGAGTTTTACAATGACAAGTTTCAAAAGGGAGGTAAAGAAAGACTCTACGAAATACGCAGGAGAAAACACAAGAGTAATGCAAAAGCAATCGAGGCCACACATCGAGATGATCTCGACGAAGATGAATGGTCCTGGTCTACTTCATCGTCAACCGATCATTACGCGACACTTGTTGACGAAAACAAGAGGCTGAAGAAGGAGAAGGGAGCGTTGAGGTTTGAGGTGAAGAACATGAAGAGGAAATGCAGAAAGCTTATTGATTTGATAGGAAAGTACGAATTGGGCATTAATGGGTATGATAAGAAGGAAGATGAGATGATGGTAATGAAGCCAAACTTGAAACTATTTGGGGTTAAGTTGGAGGTTGGGGAAGAAGATGAAATGGTGAGGCAAAGCAAGAGGAAGAGATGA

mRNA sequence

TATATCTTCTTCTTCCTTTCTGCTTCCACTTCATTTCTCTCATGGGGGAGCAGCCAGCTATAGGCCTAGGTGATGTGTGTCAAAAGCCAAAGCCCTCTCCGCCGCCCTTCTTGGCCAAAACCTACCTGTTGGTCGAGGATCCGACCACCGATGATGTCATCTCCTGGAATGACGATGGGACGGCGCTCGTCGTGTGGCATCCCCCAGAGTTCGCTAGAGATCTCCTCCCTACCCTCTTCAAACATAGCAACTTCTCTAGCTTTGTTCGCCAACTAAACACATATGGATTTCGCAAGATTGCGACAAGTCGATGGGAGTTTTACAATGACAAGTTTCAAAAGGGAGGTAAAGAAAGACTCTACGAAATACGCAGGAGAAAACACAAGAGTAATGCAAAAGCAATCGAGGCCACACATCGAGATGATCTCGACGAAGATGAATGGTCCTGGTCTACTTCATCGTCAACCGATCATTACGCGACACTTGTTGACGAAAACAAGAGGCTGAAGAAGGAGAAGGGAGCGTTGAGGTTTGAGGTGAAGAACATGAAGAGGAAATGCAGAAAGCTTATTGATTTGATAGGAAAGTACGAATTGGGCATTAATGGGTATGATAAGAAGGAAGATGAGATGATGGTAATGAAGCCAAACTTGAAACTATTTGGGGTTAAGTTGGAGGTTGGGGAAGAAGATGAAATGGTGAGGCAAAGCAAGAGGAAGAGATGA

Coding sequence (CDS)

ATGGGGGAGCAGCCAGCTATAGGCCTAGGTGATGTGTGTCAAAAGCCAAAGCCCTCTCCGCCGCCCTTCTTGGCCAAAACCTACCTGTTGGTCGAGGATCCGACCACCGATGATGTCATCTCCTGGAATGACGATGGGACGGCGCTCGTCGTGTGGCATCCCCCAGAGTTCGCTAGAGATCTCCTCCCTACCCTCTTCAAACATAGCAACTTCTCTAGCTTTGTTCGCCAACTAAACACATATGGATTTCGCAAGATTGCGACAAGTCGATGGGAGTTTTACAATGACAAGTTTCAAAAGGGAGGTAAAGAAAGACTCTACGAAATACGCAGGAGAAAACACAAGAGTAATGCAAAAGCAATCGAGGCCACACATCGAGATGATCTCGACGAAGATGAATGGTCCTGGTCTACTTCATCGTCAACCGATCATTACGCGACACTTGTTGACGAAAACAAGAGGCTGAAGAAGGAGAAGGGAGCGTTGAGGTTTGAGGTGAAGAACATGAAGAGGAAATGCAGAAAGCTTATTGATTTGATAGGAAAGTACGAATTGGGCATTAATGGGTATGATAAGAAGGAAGATGAGATGATGGTAATGAAGCCAAACTTGAAACTATTTGGGGTTAAGTTGGAGGTTGGGGAAGAAGATGAAATGGTGAGGCAAAGCAAGAGGAAGAGATGA

Protein sequence

MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKAIEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLIGKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR

Homology

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BLAST of Carg26080 vs. NCBI nr
Match: KAG6596964.1 (Heat stress transcription factor B-3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028437.1 Heat stress transcription factor B-3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 462.6 bits (1189), Expect = 1.9e-126
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 60
           MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD
Sbjct: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 60

Query: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA 120
           LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA
Sbjct: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA 120

Query: 121 IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLI 180
           IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLI
Sbjct: 121 IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLI 180

Query: 181 GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 228
           GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR
Sbjct: 181 GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 227

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BLAST of Carg26080 vs. NCBI nr
Match: XP_022938527.1 (heat stress transcription factor B-3-like [Cucurbita moschata])

HSP 1 Score: 461.1 bits (1185), Expect = 5.6e-126
Identity = 226/227 (99.56%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 60
           MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD
Sbjct: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 60

Query: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA 120
           LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA
Sbjct: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA 120

Query: 121 IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLI 180
           IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCR+LIDLI
Sbjct: 121 IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRELIDLI 180

Query: 181 GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 228
           GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR
Sbjct: 181 GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 227

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BLAST of Carg26080 vs. NCBI nr
Match: XP_023521033.1 (heat stress transcription factor B-3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 459.1 bits (1180), Expect = 2.1e-125
Identity = 225/227 (99.12%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 60
           MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWN+DGTALVVWHPPEFARD
Sbjct: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNNDGTALVVWHPPEFARD 60

Query: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA 120
           LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA
Sbjct: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA 120

Query: 121 IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLI 180
           IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCR+LIDLI
Sbjct: 121 IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRELIDLI 180

Query: 181 GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 228
           GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR
Sbjct: 181 GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 227

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BLAST of Carg26080 vs. NCBI nr
Match: XP_022973345.1 (heat stress transcription factor B-3-like [Cucurbita maxima])

HSP 1 Score: 424.1 bits (1089), Expect = 7.7e-115
Identity = 210/234 (89.74%), Postives = 217/234 (92.74%), Query Frame = 0


Query: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 60
           MGEQPAIGLGDVCQKP PSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHP EFARD
Sbjct: 1   MGEQPAIGLGDVCQKPNPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPLEFARD 60

Query: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRK------- 120
           LLPTLFKHSNFSSFVRQLNTYGFRKI TSRWEFYNDKFQKGGKERLYEI RRK       
Sbjct: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIVTSRWEFYNDKFQKGGKERLYEICRRKAWSNKPQ 120

Query: 121 HKSNAKAIEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKC 180
           HKSNAKAI+ATH+D+LDEDEWSW   SSTDHY TLVDENKRLKKEKG LRFE+KNMK+KC
Sbjct: 121 HKSNAKAIQATHQDELDEDEWSWLNPSSTDHYVTLVDENKRLKKEKGVLRFELKNMKKKC 180

Query: 181 RKLIDLIGKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 228
           R+LI LIGKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQ+KRKR
Sbjct: 181 RELIGLIGKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQNKRKR 234

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BLAST of Carg26080 vs. NCBI nr
Match: XP_038906335.1 (heat stress transcription factor B-3-like [Benincasa hispida])

HSP 1 Score: 309.7 bits (792), Expect = 2.1e-80
Identity = 162/235 (68.94%), Postives = 187/235 (79.57%), Query Frame = 0


Query: 7   IGLGDVCQK------PKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 66
           + LG+VC+K       KP+PPPFLAKTY+LVEDP TDDVISWN DGTA +VW P EFA D
Sbjct: 6   VDLGNVCEKGYVHAARKPTPPPFLAKTYMLVEDPMTDDVISWNSDGTAFIVWRPSEFATD 65

Query: 67  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRR-------RK 126
           LLPTLFKH+NFSSFVRQLNTYGFRKIATSRWEF NDKFQKG KERL EIRR       RK
Sbjct: 66  LLPTLFKHNNFSSFVRQLNTYGFRKIATSRWEFCNDKFQKGCKERLCEIRRRKAWSNKRK 125

Query: 127 HKSNAKAIEATHRDDLDEDEWSWSTSSST-DHYATLVDENKRLKKEKGALRFEVKNMKRK 186
           H +NAK I+ TH+DD +ED+ S STSSS+ D Y TLVDENKRLKKE G L FE+ NMK+K
Sbjct: 126 HSNNAKVIQVTHQDDHNEDQRSPSTSSSSDDQYTTLVDENKRLKKENGVLSFELTNMKKK 185

Query: 187 CRKLIDLIGKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 228
           CR+L+DL+  YE  I   ++KE + ++ KPN+KLFGVKLEV EEDEMVRQ+KRKR
Sbjct: 186 CRELLDLVAWYEFAIINGNEKEGDDILRKPNMKLFGVKLEVEEEDEMVRQNKRKR 240

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BLAST of Carg26080 vs. ExPASy Swiss-Prot
Match: O22230 (Heat stress transcription factor B-3 OS=Arabidopsis thaliana OX=3702 GN=HSFB3 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 3.2e-47
Identity = 114/214 (53.27%), Postives = 138/214 (64.49%), Query Frame = 0


Query: 20  PPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFVRQLN 79
           PPPFL KTY +VEDPTTD VISWN+ GT  VVW P EFARDLLPTLFKH NFSSFVRQLN
Sbjct: 38  PPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCNFSSFVRQLN 97

Query: 80  TYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRK------HKSNAKAIEAT-------HR 139
           TYGFRK+ T RWEF N+ F+KG +E +  IRRRK      +KSN + +  T       H+
Sbjct: 98  TYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTTTMVNQEGHQ 157

Query: 140 ----DDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLIGK 199
               D   ED+ S +TSSS   Y  L+DENK LK E   L  E+   K+KC++L++L+ +
Sbjct: 158 RIGIDHHHEDQQSSATSSSF-VYTALLDENKCLKNENELLSCELGKTKKKCKQLMELVER 217

Query: 200 Y----ELGINGYDKKEDEMMVMKPNLKLFGVKLE 213
           Y    E   +  D +EDE       LKLFGVKLE
Sbjct: 218 YRGEDEDATDESDDEEDE------GLKLFGVKLE 244

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BLAST of Carg26080 vs. ExPASy Swiss-Prot
Match: Q6Z9C8 (Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.1e-36
Identity = 97/212 (45.75%), Postives = 118/212 (55.66%), Query Frame = 0


Query: 6   AIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTL 65
           A G+G V Q+ +  P PFL KTY LV+DP  DDVISWNDDG+  VVW P EFARDLLP  
Sbjct: 33  AAGVG-VGQQQRTVPTPFLTKTYQLVDDPAVDDVISWNDDGSTFVVWRPAEFARDLLPKY 92

Query: 66  FKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKAIE--- 125
           FKH+NFSSFVRQLNTYGFRKI   RWEF ND F++G +  L EI RRK    A A     
Sbjct: 93  FKHNNFSSFVRQLNTYGFRKIVPDRWEFANDCFRRGERRLLCEIHRRKVTPPAPAATTAA 152

Query: 126 -----------ATHRDD---LDEDEWSWSTSSSTDHYATLV-----------------DE 184
                       T RD    L  +E   S+SSS +    L                  DE
Sbjct: 153 VAAAIPMALPVTTTRDGSPVLSGEEQVISSSSSPEPPLVLPQAPSGSGSGGVASGDVGDE 212

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BLAST of Carg26080 vs. ExPASy Swiss-Prot
Match: P22335 (Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.1e-35
Identity = 85/187 (45.45%), Postives = 113/187 (60.43%), Query Frame = 0


Query: 17  KPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFVR 76
           + +P PFL KTY LV+D  TDDVISWN+ GT  VVW   EFA+DLLP  FKH+NFSSFVR
Sbjct: 4   RTAPAPFLLKTYQLVDDAATDDVISWNEIGTTFVVWKTAEFAKDLLPKYFKHNNFSSFVR 63

Query: 77  QLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSN------AKAIEATHRDDLD 136
           QLNTYGFRKI   +WEF N+ F++G KE L  IRRRK  ++      + A  A+   D  
Sbjct: 64  QLNTYGFRKIVPDKWEFANENFKRGQKELLTAIRRRKTVTSTPAGGKSVAAGASASPDNS 123

Query: 137 EDEWSWSTSSSTD--------------HYATLVDENKRLKKEKGALRFEVKNMKRKCRKL 184
            D+   S++SS D               +  L DEN++LKK+   L  E+   K++C +L
Sbjct: 124 GDDIGSSSTSSPDSKNPGSVDTPGKLSQFTDLSDENEKLKKDNQMLSSELVQAKKQCNEL 183

[Close]

BLAST of Carg26080 vs. ExPASy Swiss-Prot
Match: Q7XRX3 (Heat stress transcription factor B-2a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2A PE=2 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 1.1e-34
Identity = 98/231 (42.42%), Postives = 124/231 (53.68%), Query Frame = 0


Query: 16  PKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFV 75
           P    PPFL KTY +VEDP+TD+ ISWND GTA VVW P EFARDLLP  FKHSNFSSFV
Sbjct: 4   PAAGTPPFLTKTYAMVEDPSTDETISWNDSGTAFVVWRPAEFARDLLPKHFKHSNFSSFV 63

Query: 76  RQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA-------------IE 135
           RQLNTYGF+K+   RWEF ND F++G K  L  I+RRK      A             I 
Sbjct: 64  RQLNTYGFKKVVADRWEFANDCFRRGEKHLLGGIQRRKGSGTGGAGAAPAGGIPTAIPIS 123

Query: 136 ATHRDDLDEDEWSWS--------TSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCR 195
           +       E   S S         +  +   A L +EN RL++E   L  E+   +R C 
Sbjct: 124 SPPTSSGGEPAVSSSPPRGAAGIAAGVSGAVAELEEENARLRRENARLARELARARRVCD 183

Query: 196 KLIDLIGKYELGINGYDKKEDEMMVMKPNLKLFGVKL-------EVGEEDE 219
            +  L+ +Y+    G +++  E  V KP   LFGV +       E GE++E
Sbjct: 184 GVRRLVSRYDHDHGGGEEEAGEGDV-KP--MLFGVAIGGKRSREENGEDEE 231

[Close]

BLAST of Carg26080 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 5.3e-34
Identity = 67/97 (69.07%), Postives = 74/97 (76.29%), Query Frame = 0


Query: 17  KPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFVR 76
           KP P PFL KTY LV+DP TD ++SW +D T  VVW PPEFARDLLP  FKH+NFSSFVR
Sbjct: 29  KPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 77  QLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRK 114
           QLNTYGFRKI   RWEF N+ F+KG K  L EI RRK
Sbjct: 89  QLNTYGFRKIVADRWEFANEFFRKGAKHLLAEIHRRK 125

[Close]

BLAST of Carg26080 vs. ExPASy TrEMBL
Match: A0A6J1FEA5 (heat stress transcription factor B-3-like OS=Cucurbita moschata OX=3662 GN=LOC111444732 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 2.7e-126
Identity = 226/227 (99.56%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 60
           MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD
Sbjct: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 60

Query: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA 120
           LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA
Sbjct: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKA 120

Query: 121 IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLI 180
           IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCR+LIDLI
Sbjct: 121 IEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRELIDLI 180

Query: 181 GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 228
           GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR
Sbjct: 181 GKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 227

[Close]

BLAST of Carg26080 vs. ExPASy TrEMBL
Match: A0A6J1IB47 (heat stress transcription factor B-3-like OS=Cucurbita maxima OX=3661 GN=LOC111471901 PE=3 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 3.7e-115
Identity = 210/234 (89.74%), Postives = 217/234 (92.74%), Query Frame = 0


Query: 1   MGEQPAIGLGDVCQKPKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARD 60
           MGEQPAIGLGDVCQKP PSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHP EFARD
Sbjct: 1   MGEQPAIGLGDVCQKPNPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPLEFARD 60

Query: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRK------- 120
           LLPTLFKHSNFSSFVRQLNTYGFRKI TSRWEFYNDKFQKGGKERLYEI RRK       
Sbjct: 61  LLPTLFKHSNFSSFVRQLNTYGFRKIVTSRWEFYNDKFQKGGKERLYEICRRKAWSNKPQ 120

Query: 121 HKSNAKAIEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKC 180
           HKSNAKAI+ATH+D+LDEDEWSW   SSTDHY TLVDENKRLKKEKG LRFE+KNMK+KC
Sbjct: 121 HKSNAKAIQATHQDELDEDEWSWLNPSSTDHYVTLVDENKRLKKEKGVLRFELKNMKKKC 180

Query: 181 RKLIDLIGKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQSKRKR 228
           R+LI LIGKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQ+KRKR
Sbjct: 181 RELIGLIGKYELGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEMVRQNKRKR 234

[Close]

BLAST of Carg26080 vs. ExPASy TrEMBL
Match: A0A5A7UDG6 (Heat stress transcription factor B-3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G002220 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.5e-79
Identity = 163/241 (67.63%), Postives = 192/241 (79.67%), Query Frame = 0


Query: 1   MGEQPAIGLGDVCQK-----PKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPP 60
           MGE   + LG+VC++      +P+P PFL KTY+LVEDP TD+VISWN DGT  +VW PP
Sbjct: 1   MGE-AMVSLGNVCEESIAVVSEPTPSPFLTKTYMLVEDPMTDNVISWNSDGTTFIVWQPP 60

Query: 61  EFARDLLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRR---- 120
           EFA DLLPTLFKH+NFSSFVRQLNTYGFRKIATSRWEFYNDKF+KG KERL EIRR    
Sbjct: 61  EFAIDLLPTLFKHNNFSSFVRQLNTYGFRKIATSRWEFYNDKFKKGCKERLCEIRRRKAW 120

Query: 121 ---RKHKSNAKAIEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKN 180
              RKH SNAKAI+ TH+D+ DED+ S STSSS D Y  L  ENK+LKKE G L FE+ N
Sbjct: 121 TNKRKHNSNAKAIQVTHQDNHDEDQRSLSTSSSDDQYTMLAYENKKLKKENGVLSFELTN 180

Query: 181 MKRKCRKLIDLIGKYE-LGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEM-VRQSKRK 228
           MK+KCR+L+DL+ KYE + ING +KKEDE+M +KPNLKLFGV+LEV EEDEM ++Q+KRK
Sbjct: 181 MKKKCRELLDLVAKYEFMVINGNEKKEDEIM-LKPNLKLFGVQLEVEEEDEMEIKQNKRK 239

[Close]

BLAST of Carg26080 vs. ExPASy TrEMBL
Match: A0A1S3B7P7 (heat stress transcription factor B-3-like OS=Cucumis melo OX=3656 GN=LOC103486932 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.5e-79
Identity = 163/241 (67.63%), Postives = 192/241 (79.67%), Query Frame = 0


Query: 1   MGEQPAIGLGDVCQK-----PKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPP 60
           MGE   + LG+VC++      +P+P PFL KTY+LVEDP TD+VISWN DGT  +VW PP
Sbjct: 1   MGE-AMVSLGNVCEESIAVVSEPTPSPFLTKTYMLVEDPMTDNVISWNSDGTTFIVWQPP 60

Query: 61  EFARDLLPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRR---- 120
           EFA DLLPTLFKH+NFSSFVRQLNTYGFRKIATSRWEFYNDKF+KG KERL EIRR    
Sbjct: 61  EFAIDLLPTLFKHNNFSSFVRQLNTYGFRKIATSRWEFYNDKFKKGCKERLCEIRRRKAW 120

Query: 121 ---RKHKSNAKAIEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKN 180
              RKH SNAKAI+ TH+D+ DED+ S STSSS D Y  L  ENK+LKKE G L FE+ N
Sbjct: 121 TNKRKHNSNAKAIQVTHQDNHDEDQRSLSTSSSDDQYTMLAYENKKLKKENGVLSFELTN 180

Query: 181 MKRKCRKLIDLIGKYE-LGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEM-VRQSKRK 228
           MK+KCR+L+DL+ KYE + ING +KKEDE+M +KPNLKLFGV+LEV EEDEM ++Q+KRK
Sbjct: 181 MKKKCRELLDLVAKYEFMVINGNEKKEDEIM-LKPNLKLFGVQLEVEEEDEMEIKQNKRK 239

[Close]

BLAST of Carg26080 vs. ExPASy TrEMBL
Match: A0A5D3DQH0 (Heat stress transcription factor B-3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007230 PE=3 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.5e-79
Identity = 160/235 (68.09%), Postives = 189/235 (80.43%), Query Frame = 0


Query: 7   IGLGDVCQK-----PKPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDL 66
           + LG+VC++      +P+P PFL KTY+LVEDP TD+VISWN DGT  +VW PPEFA DL
Sbjct: 2   VSLGNVCEESIAVVSEPTPSPFLTKTYMLVEDPMTDNVISWNSDGTTFIVWQPPEFAIDL 61

Query: 67  LPTLFKHSNFSSFVRQLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRR-------RKH 126
           LPTLFKH+NFSSFVRQLNTYGFRKIATSRWEFYNDKF+KG KERL EIRR       RKH
Sbjct: 62  LPTLFKHNNFSSFVRQLNTYGFRKIATSRWEFYNDKFKKGCKERLCEIRRRKAWTNKRKH 121

Query: 127 KSNAKAIEATHRDDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCR 186
            SNAKAI+ TH+D+ DED+ S STSSS D Y  L  ENK+LKKE G L FE+ NMK+KCR
Sbjct: 122 NSNAKAIQVTHQDNHDEDQRSLSTSSSDDQYTMLAYENKKLKKENGVLSFELTNMKKKCR 181

Query: 187 KLIDLIGKYE-LGINGYDKKEDEMMVMKPNLKLFGVKLEVGEEDEM-VRQSKRKR 228
           +L+DL+ KYE + ING +KKEDE+M +KPNLKLFGV+LEV EEDEM ++Q+KRKR
Sbjct: 182 ELLDLVAKYEFMVINGNEKKEDEIM-LKPNLKLFGVQLEVEEEDEMEIKQNKRKR 235

[Close]

BLAST of Carg26080 vs. TAIR 10
Match: AT2G41690.1 (heat shock transcription factor B3 )

HSP 1 Score: 189.9 bits (481), Expect = 2.3e-48
Identity = 114/214 (53.27%), Postives = 138/214 (64.49%), Query Frame = 0


Query: 20  PPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFVRQLN 79
           PPPFL KTY +VEDPTTD VISWN+ GT  VVW P EFARDLLPTLFKH NFSSFVRQLN
Sbjct: 38  PPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCNFSSFVRQLN 97

Query: 80  TYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRK------HKSNAKAIEAT-------HR 139
           TYGFRK+ T RWEF N+ F+KG +E +  IRRRK      +KSN + +  T       H+
Sbjct: 98  TYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTTTMVNQEGHQ 157

Query: 140 ----DDLDEDEWSWSTSSSTDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLIGK 199
               D   ED+ S +TSSS   Y  L+DENK LK E   L  E+   K+KC++L++L+ +
Sbjct: 158 RIGIDHHHEDQQSSATSSSF-VYTALLDENKCLKNENELLSCELGKTKKKCKQLMELVER 217

Query: 200 Y----ELGINGYDKKEDEMMVMKPNLKLFGVKLE 213
           Y    E   +  D +EDE       LKLFGVKLE
Sbjct: 218 YRGEDEDATDESDDEEDE------GLKLFGVKLE 244

[Close]

BLAST of Carg26080 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 142.9 bits (359), Expect = 3.2e-34
Identity = 67/97 (69.07%), Postives = 73/97 (75.26%), Query Frame = 0


Query: 17  KPSPPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFVR 76
           K  P PFL KTY LV+DP TD V+SW DD T  VVW PPEFARDLLP  FKH+NFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 77  QLNTYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRK 114
           QLNTYGFRKI   RWEF N+ F++G K  L EI RRK
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRK 125

[Close]

BLAST of Carg26080 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 141.4 bits (355), Expect = 9.2e-34
Identity = 67/104 (64.42%), Postives = 77/104 (74.04%), Query Frame = 0


Query: 20  PPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFVRQLN 79
           P PFL KTY LVEDP  D++ISWN+DGT  +VW P EFARDLLP  FKH+NFSSFVRQLN
Sbjct: 57  PTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSSFVRQLN 116

Query: 80  TYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKAIEA 124
           TYGFRK+   RWEF ND F++G K  L +I+RRK    A A  A
Sbjct: 117 TYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAA 160

[Close]

BLAST of Carg26080 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 140.2 bits (352), Expect = 2.1e-33
Identity = 80/177 (45.20%), Postives = 110/177 (62.15%), Query Frame = 0


Query: 20  PPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFVRQLN 79
           P PFL+KTY LV+D +TDDV+SWN++GTA VVW   EFA+DLLP  FKH+NFSSF+RQLN
Sbjct: 12  PAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSFIRQLN 71

Query: 80  TYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKH---KSNAKAIEATHRDDLD----ED 139
           TYGFRK    +WEF ND F++GG++ L +IRRRK     +  K +      + +    +D
Sbjct: 72  TYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVGSPSESNSGGGDD 131

Query: 140 EWSWSTSSS---------TDHYATLVDENKRLKKEKGALRFEVKNMKRKCRKLIDLI 181
             S STSS           +  A L  EN++LK+E   L  E+   K++  +L+  +
Sbjct: 132 HGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENNNLSSELAAAKKQRDELVTFL 188

[Close]

BLAST of Carg26080 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 137.5 bits (345), Expect = 1.3e-32
Identity = 79/190 (41.58%), Postives = 109/190 (57.37%), Query Frame = 0


Query: 20  PPPFLAKTYLLVEDPTTDDVISWNDDGTALVVWHPPEFARDLLPTLFKHSNFSSFVRQLN 79
           P PFL KT+ LVED + DDVISWN+DG++ +VW+P +FA+DLLP  FKH+NFSSFVRQLN
Sbjct: 21  PTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSFVRQLN 80

Query: 80  TYGFRKIATSRWEFYNDKFQKGGKERLYEIRRRKHKSNAKAIEA---------------- 139
           TYGF+K+   RWEF ND F++G K  L EI+RRK  +  + + A                
Sbjct: 81  TYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITTTHQTVVAPSSEQRNQTMVVSPSN 140

Query: 140 THRDDLDEDEWSWSTSSSTDHY----------ATLVDENKRLKKEKGALRFEVKNMKRKC 184
           +  D+ +    S S SS   H             L++EN++L+ +   L  E+  MK  C
Sbjct: 141 SGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENEKLRSQNIQLNRELTQMKSIC 200

The following BLAST results are available for this feature:

BLAST of Carg26080 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596964.1	1.9e-126	100.00	Heat stress transcription factor B-3, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_022938527.1	5.6e-126	99.56	heat stress transcription factor B-3-like [Cucurbita moschata]	[more]
XP_023521033.1	2.1e-125	99.12	heat stress transcription factor B-3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022973345.1	7.7e-115	89.74	heat stress transcription factor B-3-like [Cucurbita maxima]	[more]
XP_038906335.1	2.1e-80	68.94	heat stress transcription factor B-3-like [Benincasa hispida]	[more]

BLAST of Carg26080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22230	3.2e-47	53.27	Heat stress transcription factor B-3 OS=Arabidopsis thaliana OX=3702 GN=HSFB3 PE...	[more]
Q6Z9C8	1.1e-36	45.75	Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
P22335	2.1e-35	45.45	Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1	[more]
Q7XRX3	1.1e-34	42.42	Heat stress transcription factor B-2a OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q7XHZ0	5.3e-34	69.07	Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]

BLAST of Carg26080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FEA5	2.7e-126	99.56	heat stress transcription factor B-3-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IB47	3.7e-115	89.74	heat stress transcription factor B-3-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A5A7UDG6	1.5e-79	67.63	Heat stress transcription factor B-3-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3B7P7	1.5e-79	67.63	heat stress transcription factor B-3-like OS=Cucumis melo OX=3656 GN=LOC10348693...	[more]
A0A5D3DQH0	2.5e-79	68.09	Heat stress transcription factor B-3-like OS=Cucumis melo var. makuwa OX=1194695...	[more]

BLAST of Carg26080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G41690.1	2.3e-48	53.27	heat shock transcription factor B3	[more]
AT1G46264.1	3.2e-34	69.07	heat shock transcription factor B4	[more]
AT4G11660.1	9.2e-34	64.42	winged-helix DNA-binding transcription factor family protein	[more]
AT4G36990.1	2.1e-33	45.20	heat shock factor 4	[more]
AT5G62020.1	1.3e-32	41.58	heat shock transcription factor B2A	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 152..172
None	No IPR available	PANTHER	PTHR10015:SF285	HEAT STRESS TRANSCRIPTION FACTOR B-3	coord: 17..223
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PRINTS	PR00056	HSFDOMAIN	coord: 23..46 score: 48.93 coord: 61..73 score: 68.79 coord: 74..86 score: 84.62
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 19..112 e-value: 3.4E-51 score: 186.1
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 23..112 e-value: 5.5E-30 score: 103.8
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PROSITE	PS00434	HSF_DOMAIN	coord: 62..86
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 15..113 e-value: 5.0E-35 score: 122.1
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 17..223
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 19..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26080-RA	Carg26080-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding