Carg25838 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25838
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Uroporphyrinogen-III synthase
Location	Carg_Chr12: 3073718 .. 3078385 (-)
RNA-Seq Expression	Carg25838
Synteny	Carg25838

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAACACGTTTCCCCATGGCTCCAGTTTCTCTCTCTTTGCTCTCTCATTCGCCTTCAGTTCTTCTTCTTCCTTCTCCTAGTCTGCAACTCTCTCGCCCAATCTTACTTCCTCCTAGAATGCTCCCTTCTTGTTCCTCTACTTCAATTTCTTCTGTTTCGATTACGTCTTCCTCGATTTCGCCGCCGAAAGTTGTTGTTACCAGAGAGCGAGGCAAGAATGGGAAGCTCGTTAGTGCTCTGGTAATTTCTTGATTTTGTAATCTGAGGTTGTTTTGTTCGGATTGTTGATTTGTTGTATTCTCGTTGATTGCTCGAGGCTATTTTTGTTTTGTTTTTTTTCTTGTTCGCGAGTTAGATTTTAGTTCTACTGAGGCATTGTGTTTGTGTATTGAATTAATGTGGTGGTGCGATTAGTAAGTTTATGCGTTTGAGTGAATGTTGAAATGAATGCTGGTTGTTTGAGTGTTTTGATTCATTTTTGCCTTCTCAAGTTCTTTACCGATCGTGGAGATCTTTCCGAAATGGTTGATCATTTTGAGACTGACTGCTACTTTTACTCTTGCTGGAATATTGCTTTTAGGAGACAACAAACGCTTACTTTAGTGGATGATTCTTGACATTGCGTAAATTGGAACTGTAATGCTTTTTAAGCACCAATGTAGTTTTGGTTTTCCAGAAAGTAAAATATCTTCAATCACCAGTTGTGAGAAAATGACGCCTCTAAAATCAAATAGGCTGATCTCGTAAATAGCCACAATATGAGTTATATTTCAGTTCTTAGGTACACTCGGAGTCTCAGGACTATATTGAACCATTCCAGAGTCGCGCACGGTTGTAAACTACTTGTTAATCTCGATTCCTTTTTTGATATAATAGCTTATTCGTAATTGCTGCGCTGCAATATCAGTCTTAGGAAGCATTGATATTAAAAATGTTGATATTCTCTTAATTGAATCATGAAACTGCACCAAGTCCTTGTCTCCTTTTCCAATATGCACATTGTACTTGCATGGTGTTAATGCTTTAATAGGTGGAATATTTGAATGAAATCCTGTTAATTGCTTAGACTCGCATAGTTTTTCAGCCAGAGAACTGATCTTTTGTGTTAATCAAGAATAAACAGTGTTTAGGATGGAGAATAGCCGTGATGGTTTATCTTGAGCTCTTGTTCATTATCATTCAGACATCCACTCATGAATAAGTATTTCTGACATGGACGCATTGTTGCTTAAAGTAGAGTTGCTGATGTTATACCCTGCATTTTACGGCTTCTCTCCTCATTTCCCTTTTGTATCATTGATTTCATTGGCCTATTGTATTATAGACCTTTCGTGAAGTTAAAATAATGGGAAAGCAAAAGTGGTGGGCCTTGATGTTTTTGCGTGCATGAGAATGGTACAATTCAATCTTTATGTGAAATCAATAAACTGGAGCATATTGAAATTCCTGATGTTGATGACAGGCAAAACATGGAATAAACTGTTTGGAGCTTCCCCTCATTCAACACAGACAAGGACCTGACTTGAATAGGCTACAGTCTGTACTTAGTGGTAAGTCCTTCAAGATTGGGTCTCTCTTCTCGTACTCCTTCAAAGATCTCACTATTCTTGTTGAAAGGAATCGGTTCTCTCCTTCTCAAGCACGCACCCGCATAATCTTGCAGGAGTGTTTCTGCTGGCATTTATTTAACAGTAGTATTTATTAGTAATTTCAATGTAGCACAGGATATTTTGAAGACTAGAAAATGGCACAAGCCTTTACAATCTTCTTCTGTTCTTCATTAACAGATTCTGTGTTTGACTGGATTGTTATAACATCCCCTGAAGCTGGCTTGGTGTTTCTAGAGGCATGGAAGTAAGGGTTAGAACTAACTCACTAGACTCTCACCAGTCTTGTATGTCACAGAGTGTCAAATGTCAATTTTTCTTATTTACAGGTTGTTAATATTCCTTGATCTTTGCAAGCTTTGAAGTTTCTCGAGGTTTTTAAAATATGTTTTGAAAATTTTTCCTATCTCATATACTGGCAATTGTGAAACTTGTTTTTTTGGTATAGGTGTTAGGACTAGAAATTCAAACTCGGCATACCAGTTGATCTACCTCTTTAGAATTTGCTTAGTTATGGACGGTTTATTTCAAGTTACAGGTGATCCAGCCCATGGCAACACCATATTACCATTTGATAATATGAATTATTATACTAGTTCCATCAGATAAATTAAATTATTTACACCATTGAGTGCATTTTTAAGACCTTTGTTTTCAACCTGTATGCCTGCATGGTACATGAGCTTATCATTCAATTTGGAATGCGTTGTTGGTCAAGTGAGTTAAAACATGTTTCTGAATTTTATTTTACTGGTCATCTGTTTCTTTCTGTTGAATTGTATTTTGCATTGTTGCTTCTATATAATCATGATCCACGGTGCTCTATGTTTACGAATGACATGTGTACCAACTAAAGATTGGTCGATACTTTCACATCTTATTTTTCCATTAGAGCTGCTTTAGGTCATCCTTAAGTGATACCTTCGCATTATTTTGTCCTTAGGGCCGCTGGAATGCCAAAAGTCAGAATAGGTGTTGTGGGAGCTGGTACAGCTAACATTTTTGAGGAAGTGTTGCAATCGTCAAAACAACAACTTGATGTGGCGTTTGCACCTTCAAAAGGTAAGACACATGTCAGGTCATGTTTCATGCCTGACACATGATATTCATTATTTGCAGATAGTATTTGTTTGCATGATGTTATTAGTCTTTTGCCTATATCATTTACAAACTGTCTTAATTCAAATCAGATGATGATAAGACCTTGAAAAAAACTGTTGTTGTTTGGAACATCAGAACTTTAAAACACAACGTGAAAAAATAAGTCAAATGATTTAGATGGTACTTGTTCTAGTAGTTTTTCATCTTCAATTATGTCTCTGTTATGATTGTTGTTCAACAATCACAATTTGGCAGGTATTTGTAATTTCACTATTTTTTGGGAGGACACTGTAGATTTCTTATTCATATTACGTGTGTAGGCATCTGCATTTGATTTGGATTAGAATCCTTCGACAGAAATACATCGTTGTCCTACAAATATCAAAATTTGAATTTTATTTTCATCTATTATATCTTTCAGCAATAGGCAAGGTCTTGGCTTACGAGCTTCCAAAATTAGGGAATACGAAGTGCAGTGTCTTGTATCCTGCTTCCACTAAAGCAAGCAACGATATCGGTATGCAATGTACTCTTTTTCTTCTACACTCTAGGCTATTTTCCATTAGATAATGTGGTAACAATGATGTTTCTTTTATTTATTTGTTTATTAAAATAGTTACTCTTGTTTCCTCATGCCATAACTATAAGGAATTTTATTTTGACTGGAAGTATTGTAATAAGTCCAATTTTACCTAGACCAGTAAACCTAATTTCCACAACTGAAAATTTCAAAATACTTCTAACCAGATGAAAAATTTATAATAGCATTCCCTTCAAATGAGTTCGGACATCTCTATACCTTAAATTCACGCAAATTAGTAGTGTGGATAACTGCCAAGTCTCTGTGTTCTATTCTAATCACTTCATAAGTAGCTTTTGGTCTGAAGAAATCCAAGGAAGAGGAAGTTATTTATAGAAGTACTTATAATGTGTGCGAGATAGTGTTCTTCTAGCTATTTCATATGCAGACTGATTTCTCTTCGTGTATGTATAGTATATGCATATTCGACTAACGTATAGTAGGGCTATGAGTAATTTGAACCTTGGTTGGAAATATTAAATCAATTTTGAATCCCTGGTGAAAATTTTAAAATCAATTGCTATTATAGTTTAGCTTAGTCATTATTAATGATAACAAGCCACTATTATTTACAAGATATACATTCCTCCCCATTGCTCCAAATTAACAGTTGGAAAACTGAACTGGCAAGCCACAGTTTTATTCAACATTTATGTTTTCTTTTGTACACTTAATTTATGCGAACTGTAGTTGTGACTTGAAATTCTAATGTCAAGTGATCCGTTTGCAAGTATGAAGTGGCCGACTTGAACGAGTTCTTTTATCTGTTACTTTAAAATGGAAAATTGAAGTTTTAGTACTTGTTCGTCTCATTGTAGCATTAGTGTGCTGCTTTAGTATTGTTCTGTCTGTTCTAACTGAAAGCAAAATAATGGCTTTGAGAAATATAACCTTGGTGTCCCCTTTTGCAATCTTCAGAGGAGGGTCTTGCAAATCGTGGATTTGAGGTCACGAGGCTTAATACATACACTACGGTAAAGTTATCTTGTACACCACAAATCTAAAAACATGCCACACTGATTTTTAGAGGCGTATTCATCTACTTGATCATAAGTTCTTGATAAATATCTTGTATGCTTCAAATTTAGACCCTCGTTGAAGAGGTTCGTTGGACTATACTTGAATTTTTATCGATTCTGTAGTACACATTTCCAAACCCGTGGGAGTCTTAAATCGTAACACCTTGTTCGTTCCTGTACTCTCAGTAACCTGAATGAAGACTATGAAAAGAATATATGTTTCAAAGGCAATTAACTTCGTAGGCATCATAATTATTCCTGGTATCTTTCAGGCACCCGTTGATTATGTCGATCAATCCATTCTCGAGCAAGCACTTCTGTTGCCTGTTGTCGCCGTGGCTTCACCTTCTGCAGTTCGGTAA

mRNA sequence

CAACACGTTTCCCCATGGCTCCAGTTTCTCTCTCTTTGCTCTCTCATTCGCCTTCAGTTCTTCTTCTTCCTTCTCCTAGTCTGCAACTCTCTCGCCCAATCTTACTTCCTCCTAGAATGCTCCCTTCTTGTTCCTCTACTTCAATTTCTTCTGTTTCGATTACGTCTTCCTCGATTTCGCCGCCGAAAGTTGTTGTTACCAGAGAGCGAGGCAAGAATGGGAAGCTCGTTAGTGCTCTGGCAAAACATGGAATAAACTGTTTGGAGCTTCCCCTCATTCAACACAGACAAGGACCTGACTTGAATAGGCTACAGTCTGTACTTAGTGATTCTGTGTTTGACTGGATTGTTATAACATCCCCTGAAGCTGGCTTGGTGTTTCTAGAGGCATGGAAGGCCGCTGGAATGCCAAAAGTCAGAATAGGTGTTGTGGGAGCTGGTACAGCTAACATTTTTGAGGAAGTGTTGCAATCGTCAAAACAACAACTTGATGTGGCGTTTGCACCTTCAAAAGCAATAGGCAAGGTCTTGGCTTACGAGCTTCCAAAATTAGGGAATACGAAGTGCAGTGTCTTGTATCCTGCTTCCACTAAAGCAAGCAACGATATCGAGGAGGGTCTTGCAAATCGTGGATTTGAGGTCACGAGGCTTAATACATACACTACGGCACCCGTTGATTATGTCGATCAATCCATTCTCGAGCAAGCACTTCTGTTGCCTGTTGTCGCCGTGGCTTCACCTTCTGCAGTTCGGTAA

Coding sequence (CDS)

ATGGCTCCAGTTTCTCTCTCTTTGCTCTCTCATTCGCCTTCAGTTCTTCTTCTTCCTTCTCCTAGTCTGCAACTCTCTCGCCCAATCTTACTTCCTCCTAGAATGCTCCCTTCTTGTTCCTCTACTTCAATTTCTTCTGTTTCGATTACGTCTTCCTCGATTTCGCCGCCGAAAGTTGTTGTTACCAGAGAGCGAGGCAAGAATGGGAAGCTCGTTAGTGCTCTGGCAAAACATGGAATAAACTGTTTGGAGCTTCCCCTCATTCAACACAGACAAGGACCTGACTTGAATAGGCTACAGTCTGTACTTAGTGATTCTGTGTTTGACTGGATTGTTATAACATCCCCTGAAGCTGGCTTGGTGTTTCTAGAGGCATGGAAGGCCGCTGGAATGCCAAAAGTCAGAATAGGTGTTGTGGGAGCTGGTACAGCTAACATTTTTGAGGAAGTGTTGCAATCGTCAAAACAACAACTTGATGTGGCGTTTGCACCTTCAAAAGCAATAGGCAAGGTCTTGGCTTACGAGCTTCCAAAATTAGGGAATACGAAGTGCAGTGTCTTGTATCCTGCTTCCACTAAAGCAAGCAACGATATCGAGGAGGGTCTTGCAAATCGTGGATTTGAGGTCACGAGGCTTAATACATACACTACGGCACCCGTTGATTATGTCGATCAATCCATTCTCGAGCAAGCACTTCTGTTGCCTGTTGTCGCCGTGGCTTCACCTTCTGCAGTTCGGTAA

Protein sequence

MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVVVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGLVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLGNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVASPSAVR

Homology

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BLAST of Carg25838 vs. NCBI nr
Match: KAG7020556.1 (Uroporphyrinogen-III synthase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 465.3 bits (1196), Expect = 3.2e-127
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0


Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240

Query: 241 SPSAVR 247
           SPSAVR
Sbjct: 241 SPSAVR 246

[Close]

BLAST of Carg25838 vs. NCBI nr
Match: KAG6585644.1 (Uroporphyrinogen-III synthase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 465.3 bits (1196), Expect = 3.2e-127
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0


Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240

Query: 241 SPSAVR 247
           SPSAVR
Sbjct: 241 SPSAVR 246

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BLAST of Carg25838 vs. NCBI nr
Match: XP_023537361.1 (uroporphyrinogen-III synthase, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 460.7 bits (1184), Expect = 8.0e-126
Identity = 244/246 (99.19%), Postives = 244/246 (99.19%), Query Frame = 0


Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPR LPSCSSTSISSVSITSSSI PPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240

Query: 241 SPSAVR 247
           SPSAVR
Sbjct: 241 SPSAVR 246

[Close]

BLAST of Carg25838 vs. NCBI nr
Match: XP_022951382.1 (uroporphyrinogen-III synthase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 458.4 bits (1178), Expect = 4.0e-125
Identity = 243/246 (98.78%), Postives = 243/246 (98.78%), Query Frame = 0


Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLS PILLPPR LPSCSSTSISSVSITSSSISPPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSCPILLPPRTLPSCSSTSISSVSITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV VA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVTVA 240

Query: 241 SPSAVR 247
           SPSAVR
Sbjct: 241 SPSAVR 246

[Close]

BLAST of Carg25838 vs. NCBI nr
Match: XP_023002006.1 (uroporphyrinogen-III synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 448.7 bits (1153), Expect = 3.1e-122
Identity = 239/246 (97.15%), Postives = 240/246 (97.56%), Query Frame = 0


Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPR LPSCSST ISS SITSSSISPPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTLISSASITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHG+NCLELPLIQHRQGPDLNRLQSVL DSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGVNCLELPLIQHRQGPDLNRLQSVLCDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSK QLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSK-QLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV VA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVTVA 240

Query: 241 SPSAVR 247
           SPSAVR
Sbjct: 241 SPSAVR 245

[Close]

BLAST of Carg25838 vs. ExPASy Swiss-Prot
Match: O48721 (Uroporphyrinogen-III synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=UROS PE=2 SV=2)

HSP 1 Score: 258.8 bits (660), Expect = 6.0e-68
Identity = 148/254 (58.27%), Postives = 181/254 (71.26%), Query Frame = 0


Query: 4   VSLSLLSHSPSVLLLP----------SPSLQLSRPILLPPRMLPSCSSTSISSVSIT-SS 63
           ++L LLSH   +   P          S    LS+P+   P  + +  S+S+SS S + SS
Sbjct: 1   MALLLLSHCSILSFQPPLSSSSSFHSSHIQSLSKPVFASPSPIRNSISSSVSSSSSSVSS 60

Query: 64  SISPPKVVVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIV 123
           S S P+VVVTRERGKN +++ AL K+GI+ LELPLIQH +GPD +RL SVL+D  FDWI+
Sbjct: 61  SNSIPQVVVTRERGKNNQIIKALEKNGISSLELPLIQHARGPDFDRLASVLNDKSFDWII 120

Query: 124 ITSPEAGLVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVL 183
           ITSPEAG VFLEAWK A  P+V+IGVVGAGTA +FEE ++S+   L VAF PSKA GKVL
Sbjct: 121 ITSPEAGSVFLEAWKTASSPEVQIGVVGAGTARVFEEAMKSADGLLHVAFTPSKATGKVL 180

Query: 184 AYELPKLGNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQAL 243
           A ELP+    + SVLYPAS KA NDI EGL+ RGFEV RLNTYTT PV  VD  +L+QAL
Sbjct: 181 ASELPEKVGKRSSVLYPASLKAGNDIVEGLSKRGFEVVRLNTYTTVPVQSVDTVLLQQAL 240

Query: 244 LLPVVAVASPSAVR 247
             PV++VASPSAVR
Sbjct: 241 SAPVLSVASPSAVR 254

[Close]

BLAST of Carg25838 vs. ExPASy Swiss-Prot
Match: Q10QR9 (Uroporphyrinogen-III synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=UROS PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.1e-66
Identity = 130/198 (65.66%), Postives = 151/198 (76.26%), Query Frame = 0


Query: 49  ITSSSISPPKVVVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVF 108
           I  SS  PP VVVTRERGKN KL++AL KH +  LELPLI+H +GPD +RL +VL D  F
Sbjct: 39  IACSSPPPPDVVVTRERGKNAKLIAALEKHNVQSLELPLIKHVEGPDTDRLSAVLRDEKF 98

Query: 109 DWIVITSPEAGLVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAI 168
           DWI ITSPEA  VFLE WKAAG PKVRI VVGAGT  +F+EV+Q +   L+VAF+PSKA+
Sbjct: 99  DWITITSPEAAAVFLEGWKAAGNPKVRIAVVGAGTERVFDEVIQYNDGSLEVAFSPSKAM 158

Query: 169 GKVLAYELPKLGNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSIL 228
           GK LA ELP+   T C VLYPAS KA ++I+ GL+NRGFEVTRLNTYTT  V  VD  IL
Sbjct: 159 GKFLASELPRTTETTCKVLYPASAKAGHEIQNGLSNRGFEVTRLNTYTTVSVQDVDPLIL 218

Query: 229 EQALLLPVVAVASPSAVR 247
           + AL  PVVAVASPSA+R
Sbjct: 219 KPALSAPVVAVASPSALR 236

[Close]

BLAST of Carg25838 vs. ExPASy TrEMBL
Match: A0A6J1GHG9 (Uroporphyrinogen-III synthase OS=Cucurbita moschata OX=3662 GN=LOC111454223 PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.9e-125
Identity = 243/246 (98.78%), Postives = 243/246 (98.78%), Query Frame = 0


Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLS PILLPPR LPSCSSTSISSVSITSSSISPPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSCPILLPPRTLPSCSSTSISSVSITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV VA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVTVA 240

Query: 241 SPSAVR 247
           SPSAVR
Sbjct: 241 SPSAVR 246

[Close]

BLAST of Carg25838 vs. ExPASy TrEMBL
Match: A0A6J1KK69 (Uroporphyrinogen-III synthase OS=Cucurbita maxima OX=3661 GN=LOC111496019 PE=3 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 1.5e-122
Identity = 239/246 (97.15%), Postives = 240/246 (97.56%), Query Frame = 0


Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPR LPSCSST ISS SITSSSISPPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTLISSASITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHG+NCLELPLIQHRQGPDLNRLQSVL DSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGVNCLELPLIQHRQGPDLNRLQSVLCDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSK QLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSK-QLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV VA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVTVA 240

Query: 241 SPSAVR 247
           SPSAVR
Sbjct: 241 SPSAVR 245

[Close]

BLAST of Carg25838 vs. ExPASy TrEMBL
Match: A0A0A0LLB2 (Uroporphyrinogen-III synthase OS=Cucumis sativus OX=3659 GN=Csa_2G360600 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.9e-102
Identity = 213/247 (86.23%), Postives = 221/247 (89.47%), Query Frame = 0


Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSIT-SSSISPPKV 60
           MA VSLS  SH PSV ++PSP LQ    ILLP R L   SSTS+SS SIT SSSIS PKV
Sbjct: 1   MASVSLSSFSHPPSV-VVPSPCLQFYGRILLPRRTLACSSSTSVSSSSITSSSSISAPKV 60

Query: 61  VVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAG 120
           VVTRERGKNGKLV+ALA+HGINCLELPLIQHRQGPDLNRL SVLSDSVFDWIVITSPEAG
Sbjct: 61  VVTRERGKNGKLVNALARHGINCLELPLIQHRQGPDLNRLHSVLSDSVFDWIVITSPEAG 120

Query: 121 LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKL 180
           LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSK  L+VAFAPSKAIGKVLA ELPKL
Sbjct: 121 LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSK-LLEVAFAPSKAIGKVLASELPKL 180

Query: 181 GNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAV 240
           GNTKCSVLYPASTKASNDIEE L+NRGFEVTRLNTYTTAPVDYVDQ+ILEQA  LPVV V
Sbjct: 181 GNTKCSVLYPASTKASNDIEECLSNRGFEVTRLNTYTTAPVDYVDQTILEQARSLPVVTV 240

Query: 241 ASPSAVR 247
           ASPSAVR
Sbjct: 241 ASPSAVR 245

[Close]

BLAST of Carg25838 vs. ExPASy TrEMBL
Match: A0A6J1CQT7 (Uroporphyrinogen-III synthase OS=Momordica charantia OX=3673 GN=LOC111013898 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 6.6e-102
Identity = 210/249 (84.34%), Postives = 221/249 (88.76%), Query Frame = 0


Query: 1   MAPV---SLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPP 60
           MAPV   SLS LSH PSV ++PSP LQL R ILL PR  PSCSS+  +S+  TSSSIS P
Sbjct: 1   MAPVSLSSLSSLSHPPSV-VVPSPCLQLCRRILL-PRRTPSCSSS--ASIPTTSSSISAP 60

Query: 61  KVVVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPE 120
           KVVVTRERGKNGKLV+ALAKHGINCLELPLIQHRQGPDLNRL SVL DSVFDWIVITSPE
Sbjct: 61  KVVVTRERGKNGKLVNALAKHGINCLELPLIQHRQGPDLNRLHSVLGDSVFDWIVITSPE 120

Query: 121 AGLVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELP 180
           AGLVFLEAWKAAGMPKVRIGVVGAGTANIFE+VLQSS Q LDVAFAPSKAIGKVLA ELP
Sbjct: 121 AGLVFLEAWKAAGMPKVRIGVVGAGTANIFEDVLQSSNQLLDVAFAPSKAIGKVLASELP 180

Query: 181 KLGNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV 240
           KLG+TKC VLYPASTKASNDIEE L+NRGFEV RLNTYTTAPVDYVDQ++LEQA  LPVV
Sbjct: 181 KLGDTKCRVLYPASTKASNDIEEALSNRGFEVMRLNTYTTAPVDYVDQTVLEQARSLPVV 240

Query: 241 AVASPSAVR 247
            VASPSAVR
Sbjct: 241 TVASPSAVR 245

[Close]

BLAST of Carg25838 vs. ExPASy TrEMBL
Match: A0A6J1KA58 (Uroporphyrinogen-III synthase OS=Cucurbita maxima OX=3661 GN=LOC111492039 PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 5.6e-101
Identity = 208/247 (84.21%), Postives = 218/247 (88.26%), Query Frame = 0


Query: 1   MAPVSLSLLSHSPSVLLLPSPS-LQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKV 60
           MAPVSLS  SH+PSV  +PSP  LQL R ILLP R L   SST  SS SITSSSIS PKV
Sbjct: 16  MAPVSLSSFSHTPSV--VPSPCLLQLYRRILLPRRTLACFSSTLTSSASITSSSISAPKV 75

Query: 61  VVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAG 120
           VVTRERGKNGKL+ ALA+HGINCLELPLIQH QGPDLNRL SVLSDSVFDWI+ITSPEAG
Sbjct: 76  VVTRERGKNGKLIDALARHGINCLELPLIQHSQGPDLNRLHSVLSDSVFDWIIITSPEAG 135

Query: 121 LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKL 180
           LVFL+AWKAAGMPKVRIGVVGAGTANIF+EVLQSS   L+VAFAPSKAIGKVLA ELPKL
Sbjct: 136 LVFLQAWKAAGMPKVRIGVVGAGTANIFDEVLQSS-NLLEVAFAPSKAIGKVLASELPKL 195

Query: 181 GNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAV 240
           GNTKCSVLYPASTKASNDIEEGL+NRGFEV RLNTYTTAPVDYVDQ ILEQA  LPVV V
Sbjct: 196 GNTKCSVLYPASTKASNDIEEGLSNRGFEVMRLNTYTTAPVDYVDQFILEQARSLPVVTV 255

Query: 241 ASPSAVR 247
           ASPSA+R
Sbjct: 256 ASPSAIR 259

[Close]

BLAST of Carg25838 vs. TAIR 10
Match: AT2G26540.1 (uroporphyrinogen-III synthase family protein )

HSP 1 Score: 258.8 bits (660), Expect = 4.3e-69
Identity = 148/254 (58.27%), Postives = 181/254 (71.26%), Query Frame = 0


Query: 4   VSLSLLSHSPSVLLLP----------SPSLQLSRPILLPPRMLPSCSSTSISSVSIT-SS 63
           ++L LLSH   +   P          S    LS+P+   P  + +  S+S+SS S + SS
Sbjct: 1   MALLLLSHCSILSFQPPLSSSSSFHSSHIQSLSKPVFASPSPIRNSISSSVSSSSSSVSS 60

Query: 64  SISPPKVVVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIV 123
           S S P+VVVTRERGKN +++ AL K+GI+ LELPLIQH +GPD +RL SVL+D  FDWI+
Sbjct: 61  SNSIPQVVVTRERGKNNQIIKALEKNGISSLELPLIQHARGPDFDRLASVLNDKSFDWII 120

Query: 124 ITSPEAGLVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVL 183
           ITSPEAG VFLEAWK A  P+V+IGVVGAGTA +FEE ++S+   L VAF PSKA GKVL
Sbjct: 121 ITSPEAGSVFLEAWKTASSPEVQIGVVGAGTARVFEEAMKSADGLLHVAFTPSKATGKVL 180

Query: 184 AYELPKLGNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQAL 243
           A ELP+    + SVLYPAS KA NDI EGL+ RGFEV RLNTYTT PV  VD  +L+QAL
Sbjct: 181 ASELPEKVGKRSSVLYPASLKAGNDIVEGLSKRGFEVVRLNTYTTVPVQSVDTVLLQQAL 240

Query: 244 LLPVVAVASPSAVR 247
             PV++VASPSAVR
Sbjct: 241 SAPVLSVASPSAVR 254

The following BLAST results are available for this feature:

BLAST of Carg25838 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020556.1	3.2e-127	100.00	Uroporphyrinogen-III synthase, chloroplastic [Cucurbita argyrosperma subsp. argy...	[more]
KAG6585644.1	3.2e-127	100.00	Uroporphyrinogen-III synthase, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_023537361.1	8.0e-126	99.19	uroporphyrinogen-III synthase, chloroplastic isoform X1 [Cucurbita pepo subsp. p...	[more]
XP_022951382.1	4.0e-125	98.78	uroporphyrinogen-III synthase, chloroplastic [Cucurbita moschata]	[more]
XP_023002006.1	3.1e-122	97.15	uroporphyrinogen-III synthase, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Carg25838 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48721	6.0e-68	58.27	Uroporphyrinogen-III synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q10QR9	1.1e-66	65.66	Uroporphyrinogen-III synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=...	[more]

BLAST of Carg25838 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GHG9	1.9e-125	98.78	Uroporphyrinogen-III synthase OS=Cucurbita moschata OX=3662 GN=LOC111454223 PE=3...	[more]
A0A6J1KK69	1.5e-122	97.15	Uroporphyrinogen-III synthase OS=Cucurbita maxima OX=3661 GN=LOC111496019 PE=3 S...	[more]
A0A0A0LLB2	3.9e-102	86.23	Uroporphyrinogen-III synthase OS=Cucumis sativus OX=3659 GN=Csa_2G360600 PE=3 SV...	[more]
A0A6J1CQT7	6.6e-102	84.34	Uroporphyrinogen-III synthase OS=Momordica charantia OX=3673 GN=LOC111013898 PE=...	[more]
A0A6J1KA58	5.6e-101	84.21	Uroporphyrinogen-III synthase OS=Cucurbita maxima OX=3661 GN=LOC111492039 PE=3 S...	[more]

BLAST of Carg25838 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26540.1	4.3e-69	58.27	uroporphyrinogen-III synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	GENE3D	3.40.50.10090		coord: 103..212 e-value: 8.9E-19 score: 69.6
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	SUPERFAMILY	69618	HemD-like	coord: 58..246
IPR003754	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase	PFAM	PF02602	HEM4	coord: 70..246 e-value: 7.3E-33 score: 113.8
IPR003754	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase	CDD	cd06578	HemD	coord: 59..246 e-value: 3.08217E-32 score: 115.867
IPR039793	Uroporphyrinogen-III synthase	PANTHER	PTHR38042	UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTIC	coord: 8..246
None	No IPR available	PANTHER	PTHR38042:SF4	UROPORPHYRINOGEN-III SYNTHASE PROTEIN-RELATED	coord: 8..246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25838-RA	Carg25838-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
biological_process	GO:0006780	uroporphyrinogen III biosynthetic process
biological_process	GO:0033014	tetrapyrrole biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0004852	uroporphyrinogen-III synthase activity